LOCUS       KY710714               16625 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain CCUG 4677 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710714
VERSION     KY710714.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16625)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16625)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16625
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="CCUG 4677"
                     /serotype="O42"
                     /culture_collection="CCUG:4677"
                     /db_xref="taxon:585"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     gene            1..1239
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16625
                     /note="O antigen gene cluster"
     CDS             1595..2899
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99753.1"
                     /translation="MKISTSAQIKSGFIYKILFIFLTTLNALIVIKILPSEQLGVWYV
                     FMAIQAFILILNNALTPNITRQFSIGVAKANIDIITYCNLIRRIYIYVAIIIAILSIL
                     ILFFYLEPTLHIFSKKLIFLGWVFLIISILFEVYYSLYECALNGSGLFKETNKINFIS
                     RIILTILTLFILLENQKDDINLTLFCLFYFLSNIYKRIKIKRLFFSTFKTSKNTALIK
                     ESIYNYHSNKKMLYYLVYLSFISSVGGILITKSSLYILPYYSNLESIASYGLTYQLFE
                     MGFNIIFILTTMLTPMLVNLYYHQFEKIKSIYFKLKYFSLLAYVIGCIFIFYMATPLL
                     NILKVNTLILNQKQLFFLSIVFILQLNHSISGHILTIENKIPFAYPSFFSGLITLILS
                     LILIPIYHIDGAIYSILISQLIFNNWFWPLKSYFFVRKKHEI"
     CDS             2889..4037
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99754.1"
                     /translation="MKFKIILIFIIFEIISLTYIVIAGEYNGDYLHRNIKNPIYIILI
                     INISIIFLYLLYYFFWQLLDNLCSRIKIKNFNTPNLNILFFILSILYLFFYIRTGVGK
                     PGSENIELTLLDKIIFLPIIISKFNFLIFIYSAANKYKTKLYYLTLIIFLVTEILRGI
                     SFPLLIIGILESKTIFNKLRKINPIITITLLILFINLIYNLKYYIRLDDSYQYLNILT
                     TLSMFVGRLSLTSNLSYLIEHIPSISYMIESNFNYSSINEFLEKLTPAPSIFGIHEKV
                     IEFGKLIFYYNFNHLGSATASSVIGIIMILPEQIIAIVIILISSIVFINIITRLLYSS
                     YQQNCVAFIIMFLTLYQGFWGLLANYIYALTFYLFMLLVLKIFGEKNV"
     CDS             4030..4815
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99755.1"
                     /translation="MYNYKIVTLSLKDALQRRDNFEKNFSQRSSLKFSFFDGIYGKDI
                     PNIELDKIYCSSLANEKIKRELTKGEIGATYSHYLIYKEALEDNVDYIIILEDDSYIN
                     EDFDSIIKDLMYKLSPEENSIIFIQQHTLNDKVILSKKPRIEFNNKYSLYKLLGSSQY
                     FVGSYGYIVTKKALTQLVNSYLPIYCVCDHWYFIKKKCNINNFFALNNYLVSTNEEEV
                     RKIDSFINKERSILIKNRSISIIAKMKIKIKYYALRLFNKDWE"
     CDS             4812..5882
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99756.1"
                     /translation="MILIKKIFFSIIILFKIIISFLIYPFFSNKYKNTILIGTRNGEP
                     GYDNGEILFEYLKESGEKNYFLIYKNISSKIDGILKKNSIHSILRIISAKYIYITHSE
                     SDILSYLWRIFKYKKYIFIQHGVIGIKCLPEYQKKKYFKYISSNLYETEVFINHFKIE
                     KNKIIQSGLARFDKYSVNNQDICEINSCLIMFTWRKKTTYHNNINYNKIVSMLRKNNP
                     NIKIYITNHNMSKNLPNEIEHITYVKNTELPHIIKNTDLLITDYSSISWDYLYQNKCI
                     WFYHYDHNRYMADEGTYCNFASFFGYQTHENNLTDIDNIIDEIIKFNNINNTIFLKKN
                     FFYLHNEKKHMKTLLQQVEKYE"
     CDS             5875..6954
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99757.1"
                     /translation="MNNILVIATAARAGGALSILKKTISDAKKTTEKYTFLVDPSIKN
                     TLDKSDNIEYIGINTKNWIKRIYFDIFGYSKIIKNSTYNICLNLQNVPVRIKNIKQYV
                     YYHQSLPLIRYNFFKKDKKLWLYQKFYLFFVKINISYAYKFIVQTQWIYKNLYTKINF
                     PSEKVEIIEPVININKDLINTKLYDPNKNILFYPAAPYFYKNHIVIIHALLKIPESYL
                     RENNYLLILTITENEYVEIYKENIPSSLKEFIKFLGKINNQEVHHYLNISKALLFPSY
                     IETFGLPLIEAMNHGCFIIASDLEYSYDTLKKYNNKSLCSNDNIEMWANEIKSLIEKN
                     KIQYIDNSINLNKDIINFIINDNKD"
     CDS             6938..7975
                     /note="UDP-N-acetyl-glucosamine 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlA"
                     /product="fnlA"
                     /protein_id="AXY99758.1"
                     /translation="MIIRINMFNNKILLITGGTGSFGNAVLNRFLETDIKEIRIFSRD
                     EKKQDDMRKKYNNHKLKFYIGDVRDYSSILNATRGVDFIFHAAALKQVPSCEFHPIQA
                     VKTNILGTENVLEAAIANSVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNLDS
                     NKTVICGTRYGNVMASRGSVIPLFVDLMSEGKAITITDPNMTRFMMTLEDAVDLVLYA
                     FQNGNNGDIFVQKAPAATIETLAIALKELLNTPEHPINIIGTRHGEKLYEALLSREEM
                     LAAIDMGDYYRVPPDLRDLNYDKYVEQGDSRISEIEDYNSHNTTRLDVNEMKKLLLKL
                     PLIKNLHNNKI"
     CDS             7988..9088
                     /note="UDP-2-acetamido-2,6-dideoxy"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlB"
                     /product="fnlB"
                     /protein_id="AXY99759.1"
                     /translation="MKILVTGADGFIGKNLCVRLAENNFELIKIDRNSSKHDLELGLM
                     NANFIFHLAGINRPKNESEFHEGNASLTEEITHFLIENKKKTPIMLSSSIQANNNNKY
                     GESKKLAETHIKAYGLKTHANYYIYRLPNVFGKWCKPNYNSFIATFCHNIANDIDIII
                     NSPTAKVSLIYIDDLCNELISLLNGNNRIGFQNISPIYTTTVGEVAELLYAFKNSRNT
                     LVTENVGVGFTRALYSTWLSYLSPQQFTYSVPHYEDQRGVFCEMLKTQNSGQFSFFTA
                     HPGITRGGHYHHTKTEKFLIIKGKALFKFENIITGEKYELTVSDHDYKIVETVPGWSH
                     DITNIADSELIVMLWANEIFNKEFPDTIARPL"
     CDS             9088..10218
                     /note="UDP-N-acetyl-Glucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlC"
                     /product="fnlC"
                     /protein_id="AXY99760.1"
                     /translation="MKKLKVVSVVGTRPEIIRLSRVLIKLDQFCEHIIVHTGQNYDYE
                     LNEVFFTDLGVRKPDHFLNAAGANAAETIGNVIIKVDQVLEKVSPEAVLILGDTNSCI
                     SAIPAKRRKIPIFHMEAGNRCFDQRVPEETNRRIVDHTADINLTYSDIARQYLLSEGI
                     PADRVIKTGSPMFEVLSHYMEQIDKSKVLSRLGLEPGAFFVVSAHREENVDSPKHLMK
                     LVNILNTVAEKYNLPVIVSTHPRTRKRIDDHGITFHNNIQLLKPLGFHDYNHLQKHAK
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEASVMMVGLECDRVLQALNILSLQ
                     PKGNERLLRQVADYSMPNVSDKVVRIIHSYTDYVKRVVWKEY"
     CDS             10218..11429
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99761.1"
                     /translation="MRLALIIDDYLPYSTRVGAKMFHELALELQSLGHHITIITPDKD
                     QPSNLYIDKLDDITIWRFKNGPIKDVKKIQRAINETLLSYNAWKAIKHLINKDSFDGI
                     IYYSPSIFWGKLVNKLKKKCQCPSYLILRDLFPQWIVDAGILKQGSLIEQYFRFFERY
                     SYKQASIIGLMSQKNLDYFYQHNKNKGLKCEILRNWANTTQTNIDTNGYISIRESLNL
                     QNKTIFFYGGNIGHAQDMGNLMRLVKKMSVYPDAHFLFLGQGDEVELIKTLSNKWNLN
                     NFSYLPSVDQTKFKLILKEIDIGLFSLASHHSAHNFPGKLLGYMANHIPILGSVNEGN
                     DLQELVNKENAGFIHINGEDDNLFESAKKLHLDIELRKKCGNDAYNLLCKEFSVTSIA
                     STLIGRLRSLK"
     CDS             11426..11827
                     /codon_start=1
                     /transl_table=11
                     /product="wbuC"
                     /gene="wbuC"
                     /protein_id="AXY99762.1"
                     /translation="MKYIDNSSLENLYKEAKKSERLRSHLLLHKSHEDKVQRLLIALV
                     NDSYVEPHYHELPHQWEMFIILEGQIEITLYNHSGEIKEKFLAGENTNISIVNLEPNE
                     IHALKCISQKALMLEVKEGPFDPNYAKKFLI"
     CDS             11888..13054
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99763.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             13066..13569
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99764.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13645..14202)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99765.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(14202..15035)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99766.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15139..16155)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99767.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     STLF"
     gene            complement(16155..16625)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5905 a   2725 c   2587 g   5408 t
ORIGIN
        1 atgctagaac aacatatcga agcggaatta gcgcaagatc ccgctaacga tctcctttgg
       61 tggcgtcgat taattcgtgc cattgataaa tgggcccctc ctggccagcg tttaattatc
      121 gtaaccagtg aaggacgtat tatcggcgct caacgtaatg aaatgcaggt tgtcagaaac
      181 tttattggcc aatctgataa cgccgatcat cctaaaaaga aaaaatatgg tcgttctgag
      241 atgttagggc cattttcagt aagggatggt gaagatcatt atcagcttta tcttgtacgt
      301 ccatcaagta gccctcaatc tgactttatc aacttattat ttgataaacc gttattgcta
      361 ctcatattca ctatgcttat tagcacaccg ttattagtgt ggctttcttg gagcttggca
      421 aaaccggcaa gacaacttaa aaacgcagct gatgatgttg cgaaaggtaa tttacgccct
      481 catccagagc tagaaaccgg cccacaagag tttttagctg ctggtactag ttttaatcag
      541 atgataagcg cactcgaacg catggttgaa gcacagcaac gtttaatttc tgatatttct
      601 catgaattac gaactccatt aacacgctta caattagcca gtgcattgtt acgtcgccgc
      661 aacggagaaa gtaaagaatt agaacgcatt gaaactgaga ctcaacgcct tgatgggatg
      721 attaatgact tattggtgct ttcacgtaat caatataaaa atgagttgtt acgagaaact
      781 gtaaaagcaa atgagttgtg ggatgatatt ttagataacg ctaaatttga ggctgaacag
      841 agtaataaga cattacaagt gacaacacct ccaggtgcat ggcctattta ctgtaaccct
      901 tactcactcg ctagcgcatt tgaaaatatt gttcgtaatg ccttacgtta ttcaaattct
      961 cgcattgaag ttgcattcac agagcaaaat caaggcatca ccattattgt tgatgacgat
     1021 ggcccaggtg ttagccctga agacagagag catatcttcc gaccgtttta tcgcactgac
     1081 gaagcaagag atcgcgaatc aggtggtaca ggcttaggat tagcgattgt tgagacagca
     1141 gtaagtcaac atcgaggcca tgttaaagcc gatgacagcc cattaggtgg gctgagagta
     1201 gagatttggt tgccgagggg aagtgccggt aataaatagc caatagccta gatagagtaa
     1261 ttaaactgtt gtatcaaaat atcctgccat ctattgaaga ataggtggca tattcttttt
     1321 gttagcatct aatgatgcaa ttcagaatga tctggctgaa tcgaccgcta ttccgatatt
     1381 tatatatcta caatcaatgc taaaataccc agctattact tgaccgtctg gtttgaagag
     1441 tctactctca gtgcggtttt tcgcatattg ttaaaatgtg cttatataac aaaactctta
     1501 tattacaaaa agataagggt ttttcgagat aagttttggc gctctggggg ccgtaaaccc
     1561 agtgcggtag ctatgtataa ttattactaa aaatatgaaa attagtactt cagcacaaat
     1621 aaaaagtgga tttatatata aaatattatt tatatttcta acaacattaa atgcattaat
     1681 agttattaaa atacttccta gtgaacaact aggtgtttgg tatgtattta tggcaattca
     1741 agcatttatt ttaatattaa ataatgcttt aactcccaat attacccgac aattttccat
     1801 tggtgtagca aaagctaata tagatataat tacctactgt aatttaataa gaagaatata
     1861 tatatatgta gctataatta tagcaatctt atctatatta atcctctttt tttatttaga
     1921 accaacgtta catatttttt ctaaaaaatt aattttttta gggtgggtat tccttataat
     1981 atcaatatta tttgaggtct actattcact atatgaatgt gcattaaatg gctcaggatt
     2041 atttaaagaa accaataaaa ttaattttat ttctagaata atactgacca ttttgacact
     2101 atttatttta ttagaaaatc aaaaagatga tataaactta actttatttt gtttgtttta
     2161 ttttttatct aatatatata aaagaataaa aataaaaaga ctattttttt caactttcaa
     2221 aactagtaaa aacaccgctc taataaaaga aagtatatat aattaccatt caaacaaaaa
     2281 aatgctatat tatcttgtat atttatcttt tatatcaagc gtaggaggca tacttattac
     2341 aaaaagttct ttatacatat taccatatta ctctaatctt gaaagcatag catcatatgg
     2401 tttaacttat caactatttg aaatgggttt taatattatt tttatattaa caacaatgct
     2461 aacccctatg cttgttaatt tatattatca tcaatttgaa aaaataaaat caatctactt
     2521 taagcttaag tatttctcac ttttagcata tgtaataggt tgcattttta tattttatat
     2581 ggccactcca ttattaaaca tattaaaagt aaatacttta atattaaatc aaaaacaact
     2641 tttcttccta tctatagtat ttatattaca attaaatcat agtatatcag gccatatatt
     2701 aacgatagaa aataaaattc cttttgctta tccatctttt tttagtggtt taattacact
     2761 tattttatcc ttaattctaa tacctattta tcatattgat ggtgcaattt actctatatt
     2821 aatttcacaa ctaattttca acaattggtt ttggcctttg aaatcttatt tctttgtaag
     2881 aaaaaaacat gaaatttaaa ataatactta tatttataat atttgaaatt attagcctaa
     2941 catacattgt aattgcaggt gaatataatg gagactacct tcatcgcaat ataaaaaacc
     3001 ctatttatat aatacttatt attaatatta gcattatatt cttatatctc ttatattatt
     3061 ttttttggca attacttgat aatctatgta gtagaataaa aataaaaaat ttcaacacac
     3121 ctaatttaaa tattttattt tttattttat ctattctata cttatttttt tacatcagaa
     3181 ctggtgtagg taagcctggc agtgaaaata tagaattaac gctacttgat aaaatcattt
     3241 ttcttccgat aattataagt aaattcaatt ttttaatttt tatttattcg gctgcaaata
     3301 aatataaaac aaaactttac tacctcacat taataatatt tttagttaca gaaatcctta
     3361 ggggaatttc attccctcta cttattattg gaattttaga atctaaaact atctttaata
     3421 aattaagaaa aataaatcca ataataacaa taaccttact tattttattt attaatttga
     3481 tttataattt aaaatattat ataaggctag atgattcata ccaatatctt aatatactta
     3541 caacactatc tatgtttgta ggtagattga gcctaacatc aaatttgagt tatttaattg
     3601 aacatattcc atctattagc tatatgattg aatcaaattt taattactca tcaattaatg
     3661 agtttttaga aaagctaact ccggcaccaa gtatatttgg tatacacgaa aaggtaatag
     3721 agtttgggaa attaattttt tattataact ttaatcactt agggagtgca actgcttcct
     3781 ccgttattgg gattataatg attttaccag aacaaattat agcgatcgtt ataatattaa
     3841 ttagctcaat agtatttatt aatattataa cgagattatt atactcatct tatcaacaaa
     3901 attgtgtagc attcataatt atgttcttga ctctctatca gggtttttgg ggactactgg
     3961 ctaactacat ctatgcactt acattttact tatttatgtt attagtattg aaaatatttg
     4021 gtgaaaaaaa tgtataacta taaaatagta acattatcct taaaagatgc cttgcaaagg
     4081 cgtgataatt tcgaaaaaaa tttttcacaa cgttcatctt taaaatttag tttctttgac
     4141 ggtatttatg gaaaagatat tccgaatata gaattagata aaatttactg ctcatcatta
     4201 gcaaatgaga aaataaaaag agaattaaca aaaggtgaaa ttggcgcaac ctactctcat
     4261 tacttaattt ataaagaagc attagaagat aatgttgact atattattat cttagaggat
     4321 gactcttata ttaatgaaga ctttgattca ataattaaag atctaatgta taaattaagt
     4381 cctgaagaaa attcaataat ttttatacaa caacatactt taaatgataa agtaatacta
     4441 agcaaaaaac ccagaataga atttaataat aaatatagtt tatataaact attaggtagc
     4501 tctcaatatt ttgtaggctc atatggttat atcgtaacaa aaaaagctct aactcaatta
     4561 gttaattcat atctacctat atattgtgtt tgtgaccatt ggtattttat aaaaaagaaa
     4621 tgtaatataa ataacttttt tgcattaaat aattatctag tttccacaaa tgaagaagag
     4681 gtaagaaaaa tagacagttt tataaataaa gaaagatcaa tcttaattaa aaataggtct
     4741 atttcaatca ttgccaaaat gaaaataaaa ataaaatatt atgctcttag attatttaat
     4801 aaggattggg aatgatttta attaaaaaaa tatttttttc aataattatt ctatttaaga
     4861 ttattatttc atttttgatt tatccatttt tctctaataa gtataaaaat actattctta
     4921 ttggcacaag aaatggtgaa ccgggatacg acaatggaga aattcttttc gaatacctaa
     4981 aagaatctgg cgaaaaaaat tattttttaa tttataaaaa catctcatct aaaattgatg
     5041 ggattttaaa gaagaatagc attcatagta tattaagaat aataagcgct aaatatatat
     5101 atattactca ttctgagtct gatattttga gttatctatg gagaattttc aaatataaaa
     5161 aatatatttt tatccaacat ggtgttattg gtataaaatg tcttccagaa tatcagaaaa
     5221 aaaaatactt taaatatata tctagtaatt tgtatgaaac agaagttttt atcaatcatt
     5281 tcaaaataga aaaaaacaaa ataattcaat caggtttagc tagatttgat aaatattctg
     5341 ttaataatca agatatttgt gaaataaact catgcttgat tatgtttaca tggagaaaaa
     5401 aaacaactta ccataataat attaattaca ataaaatagt ttccatgtta aggaaaaata
     5461 acccaaacat aaaaatatat ataaccaacc acaatatgtc taaaaatttg cccaatgaaa
     5521 tagaacatat aacttacgtc aaaaacactg aattaccaca cataataaaa aacactgatt
     5581 tattaatcac tgattattct agtatatcat gggactatct ttatcaaaat aaatgtattt
     5641 ggttttatca ttatgatcat aatagataca tggctgacga aggaacttat tgtaattttg
     5701 cctcattttt tggttaccaa acccatgaga ataatctcac ggatatagat aatattatcg
     5761 atgaaataat taaatttaat aatataaata atactatttt tctaaagaaa aacttctttt
     5821 atttacataa tgaaaaaaaa catatgaaga cacttttaca acaagttgaa aaatatgaat
     5881 aacatattag tcattgcgac agcagcgaga gcgggtggag cacttagcat cttaaagaaa
     5941 accatttcag atgctaaaaa aacaactgaa aaatatacct tcttagttga cccatcaatc
     6001 aaaaacactt tagataaatc tgataacatt gaatatatag gaataaatac aaaaaattgg
     6061 attaaacgaa tatattttga tatttttggt tactcaaaaa taataaaaaa ttcaacttat
     6121 aatatttgtc tcaacttaca gaacgtccca gttcgaataa aaaatattaa acaatatgtt
     6181 tactaccatc aatcattacc acttattaga tataactttt ttaaaaaaga taaaaaacta
     6241 tggctttatc aaaaatttta tttatttttt gttaaaataa atatcagtta tgcatataag
     6301 tttatcgtac aaacccaatg gatttataaa aacttatata caaaaattaa ttttccttca
     6361 gagaaagtag aaataattga gcctgtaatt aatataaata aagatttaat aaatactaaa
     6421 ctttacgacc ctaataagaa tatattattt tatccagcag ctccttattt ttataaaaat
     6481 catattgtaa ttatacatgc gttattaaaa atacctgaat cttatttaag agaaaataat
     6541 tacttattga ttttaacaat cacagaaaat gaatatgtag aaatatacaa agaaaatatt
     6601 cctagctctt taaaagaatt tatcaagttt ttaggtaaaa ttaacaatca agaagtgcac
     6661 cattatctca atataagtaa ggcattactg tttccaagct atattgagac atttgggttg
     6721 cctttaatcg aagcaatgaa tcatggttgt tttatcattg catcagattt agaatattcc
     6781 tatgacacat taaaaaaata taataataaa tctctatgtt caaatgataa cattgaaatg
     6841 tgggctaatg aaattaaaag tttaatagaa aaaaacaaaa ttcaatatat agataactct
     6901 ataaatctta ataaagatat tataaacttt ataattaatg ataataagga ttaacatgtt
     6961 taacaataaa attttactaa tcactggcgg tacaggctcc tttggcaatg ctgtcttaaa
     7021 tcgcttttta gaaacagata taaaagaaat tcgtatattt tctcgcgatg aaaaaaaaca
     7081 agatgatatg cgtaaaaaat ataataatca taaattaaag ttttatattg gcgatgttcg
     7141 tgattattca agtattttaa atgcaactcg tggcgttgat tttatttttc atgcggccgc
     7201 acttaaacaa gtaccttcat gtgaatttca tccaattcaa gccgttaaaa caaatatttt
     7261 gggtacggaa aatgttttag aagctgcgat agctaatagt gtaaaaagag tcgtttgttt
     7321 gagcacagat aaagcggtgt atccgatcaa tgcaatgggt atatccaaag caatgatgga
     7381 aaaagtcatg gttgctaaat ctcgtaattt agatagcaat aaaaccgtta tttgtggaac
     7441 acgttacggt aatgtaatgg cttcacgagg ttcagttatt cctttatttg tcgatttaat
     7501 gagtgaaggt aaagctatta caattacaga tcctaatatg actcgcttta tgatgacatt
     7561 agaagatgcc gttgatcttg tattatatgc attccaaaac ggaaataatg gcgatatttt
     7621 tgttcaaaaa gcaccagcag caacaattga aacattagca atagcattaa aagaactctt
     7681 gaatacacca gaacacccaa taaatattat tggtacgcgc catggggaaa aactttacga
     7741 agctctttta agtagagaag aaatgttagc agctattgat atgggtgatt attaccgtgt
     7801 cccaccagat ttgcgtgatc ttaattatga taaatatgtt gaacaaggcg attcacgtat
     7861 ttctgaaatt gaggactata attcacataa tacgactaga ttagatgtaa atgaaatgaa
     7921 aaagctatta ttaaaattgc ctttaataaa aaacttacac aataataaaa tttaaggtca
     7981 tttgaatatg aaaatactag ttactggtgc tgatggtttt attggtaaaa atttatgtgt
     8041 tcgattagca gaaaataatt tcgaattaat aaaaatagac cgtaatagtt caaaacatga
     8101 cttagaacta ggcctgatga atgcaaactt tatctttcat ttagctggaa taaatagacc
     8161 taaaaatgaa tcagagtttc atgaaggtaa tgcatcatta actgaagaaa tcacgcattt
     8221 cctgattgaa aataagaaaa aaacaccaat aatgctgagt tcatcaattc aagcaaataa
     8281 taataataaa tatggtgaaa gcaagaaatt agcagaaact catataaaag catatgggtt
     8341 aaaaactcat gcaaactatt atatttatag attgccgaat gtttttggta aatggtgtaa
     8401 accaaattac aactcgttta ttgcaacctt ttgccataat attgcaaatg atattgatat
     8461 tatcataaac tccccaacag ccaaagtttc actaatttat attgacgatt tatgtaatga
     8521 attaatctct ctattaaatg gtaataatag aattggcttt caaaatattt cacctattta
     8581 tacaactacc gttggtgagg ttgctgaatt actttatgcc tttaaaaata gtcgcaatac
     8641 attagttaca gaaaatgtag gtgttggttt tactcgagca ctatattcca cttggctaag
     8701 ctatctttca cctcagcaat ttacttattc agttcctcat tatgaagatc aaagaggtgt
     8761 tttttgtgaa atgcttaaaa cacaaaacag tggccaattt tcttttttta ccgcacaccc
     8821 tggtattact cgtgggggac attatcacca tactaaaaca gaaaaattct taattattaa
     8881 aggaaaagct ttgtttaaat ttgagaatat tattacaggt gaaaaatacg agttaactgt
     8941 ttctgaccat gattataaaa tcgtagaaac agtaccagga tggtctcatg acattacaaa
     9001 tattgccgat agtgaattaa ttgttatgtt atgggcaaat gaaatcttta ataaagaatt
     9061 tccagataca atagcgagac ctttgtaatg aaaaaattaa aagttgtatc cgttgttggt
     9121 actcgtccag aaattattcg tctatctaga gttttaataa aattagatca attttgtgaa
     9181 catattattg ttcatactgg acaaaattac gactacgaac taaatgaagt ttttttcaca
     9241 gatttaggtg ttagaaaacc cgatcatttc ttaaatgctg ctggcgctaa tgctgcagaa
     9301 acgattggta atgtgattat aaaagttgat caagtcttag aaaaagtctc tccagaagct
     9361 gtacttattt taggtgatac aaattcttgc atttcagcaa ttccagccaa aagacgtaaa
     9421 atacctattt ttcatatgga agcaggtaat cggtgttttg atcagcgcgt accggaggaa
     9481 acaaataggc gtatcgttga tcatactgct gatatcaatt tgacctatag tgacattgct
     9541 cgtcaatatc ttctatcaga aggcatacct gcagatagag ttataaaaac aggtagtcct
     9601 atgtttgaag tactttctca ttatatggaa caaattgata aatctaaggt attatctcga
     9661 ttaggtttag aacctggagc tttttttgtt gtaagtgctc accgagagga aaatgttgac
     9721 tcgcctaagc atttaatgaa attagtcaac attttaaata ctgttgctga aaaatataat
     9781 ttaccagtta tcgtgtcgac acatccaaga actagaaaaa gaattgatga ccatggaatt
     9841 actttccata ataatattca attattaaaa ccattaggtt ttcatgatta taatcatcta
     9901 caaaaacatg caaaagcagt tctgtctgac agtggaacta tcacagaaga atcatcaatt
     9961 atgaacttcc cggctcttaa tatcagggaa gctcacgaaa ggccagaagg ctttgaggaa
    10021 gcatcagtga tgatggtcgg tttagaatgt gatcgagtgc ttcaagcatt aaatatactc
    10081 tccttacaac caaagggtaa tgaacgttta ttacgccaag tagctgatta tagtatgcct
    10141 aatgtttcag acaaagttgt aagaataatt cattcttata ctgactatgt gaaaagagtt
    10201 gtctggaaag aatactaatg cgattagctc ttatcattga cgattacttg ccctacagca
    10261 ctcgagttgg ggcaaaaatg tttcatgaat tagcattaga gctacaatca ctcggacacc
    10321 atattacaat cataactcca gataaggatc aaccatctaa tctatatatt gataaattag
    10381 atgatattac tatctggcgt tttaaaaatg gtcctattaa ggatgtaaaa aagatccaaa
    10441 gagcaattaa tgaaacacta ctttcttaca atgcttggaa agctattaaa catttaatca
    10501 ataaggattc ttttgatggc ataatttact attctccttc tatattttgg ggaaaattag
    10561 ttaataaact aaagaaaaaa tgccagtgtc cttcttattt aatattaagg gatttatttc
    10621 ctcaatggat tgttgatgct ggtattttaa agcaaggttc attgatcgaa cagtactttc
    10681 gattttttga acgttactcc tataaacaag catcaataat tggcttaatg tctcaaaaaa
    10741 atctagatta tttttatcaa cataataaaa ataaaggttt gaaatgtgaa attttaagaa
    10801 attgggcaaa tactactcaa acaaacattg atactaatgg atatatttca atacgtgaat
    10861 ctttaaactt acaaaataaa actattttct tttatggtgg aaacattggt catgcccaag
    10921 atatgggtaa tttgatgcga ctagtgaaga aaatgagtgt ttatcctgat gctcattttc
    10981 tctttcttgg acaaggcgat gaagttgaat taattaaaac tttatctaat aaatggaatt
    11041 taaataattt tagctatcta ccttcagttg atcagactaa atttaaatta attcttaaag
    11101 aaattgatat tggtttattt tcattagcgt cacatcactc tgcacataat tttccaggta
    11161 aattattagg atatatggct aatcatatcc ctatattagg tagcgttaat gaaggaaacg
    11221 acttacaaga actagtaaat aaagaaaatg caggttttat tcatataaat ggcgaagacg
    11281 ataatttatt tgaatctgct aaaaagctcc accttgatat agaactacga aaaaaatgtg
    11341 gaaatgatgc ttataatctg ttgtgtaaag agttttcagt tacttctatt gcatcgacac
    11401 taataggtag gttgagatct ttaaaatgaa atatattgat aattcatcgt tagagaattt
    11461 atataaagaa gcaaaaaaat cagaaaggtt acgttctcat ttattattac ataaatccca
    11521 tgaagacaaa gtacaacgct tacttattgc tttagtaaac gatagctatg ttgaaccaca
    11581 ttatcacgag ttacctcatc aatgggaaat gtttattatt ttggaagggc aaattgaaat
    11641 tacactatat aaccattctg gagaaataaa agaaaaattc ttagctggag aaaatactaa
    11701 tatttcgatt gtaaacttag aaccaaatga aatacacgct cttaaatgca tctctcaaaa
    11761 agcattaatg ttggaagtaa aagaggggcc ttttgatccc aattatgcta aaaaattctt
    11821 aatttaatag atcaatttac tatctataat acacctataa agttaggtta atcttaaagg
    11881 tctatttatg aaaatcacaa tctcaggtac cggctatgtc ggtctatcta acggaatact
    11941 cctttcccaa aacaacaaag ttgttgcgtt agatatcaat aaagaaaaag ttgaattact
    12001 gaataaaaaa atctctccaa ttacagataa agaaattgaa gaatttttag caaccaaaga
    12061 gcttgatttt actgcaacct tagataaaga gttcgcttat aaagatgcgg attttgttat
    12121 cattgcaaca cctaccgatt acgatcctaa aacaaattac tttaatacct cctctgtaga
    12181 gtctgttatc cgtgatgttt tagaatataa tccaaacacg acaatgatta taaaatcaac
    12241 cattcctgtt ggttttacga aagaaatgag agaaaaatat aatacggata atattatttt
    12301 ctctcctgaa ttcttacgtg aaggtcgtgc actttacgat aatttatacc catcgcgtat
    12361 tgttattggt gaacaatcag aaagagcaca aaaatttgca gaccttttac aacaaggtgc
    12421 attaaagaaa gatattgatg ttctgttcac tgactcaaca gaagcagaag ccattaaatt
    12481 atttgccaat acttatttag cgctacgtgt tgcttacttt aatgaacttg atagttatgc
    12541 tgaatcttat ggtttgaact caaaacaaat catcgaaggc gtctgcttag atcctcgtat
    12601 cggcaaccat tataataacc catcattcgg ttatggcggt tactgcttac caaaagatac
    12661 taaacagctg ttagcaaact acgactcggt gcctaataat attattagtg caatcgtcga
    12721 agctaaccgc acacgtaaag actttattgc cgactcaatt atcgctaaat caccaaaaat
    12781 cgtaggtgtt tatcgtttaa ttatgaaatc tggctctgat aatttccgtt cttcttctat
    12841 ccaaggcatt atgaaacgca ttaaagccaa agggattgag gtggtaatct atgagccaga
    12901 gatgaaagaa gagactttct ttaactcaaa attaattcgt gatttaaatg agtttaaagc
    12961 aatctctgat gtcattatta ctaatagaat gtcatctgta ctaaacgacg ttgaagaaaa
    13021 agtttatact cgtgacctct ttggtaacga ctaaatcgat tttatatgct caatatcgtc
    13081 ttatttgaac ccgaaattcc accgaatacg ggtaatatta tccgcctgtg tgctaacaca
    13141 ggctttcatc ttcatttgat ccaacctttg ggttttactt gggatgataa acgtcttcgt
    13201 cgtgcaggac ttgattatcg tgaatttgct gatattaaac agcatcacga ttactatgct
    13261 tttttagaaa gtgaaggttt aaaccctgat aatgcacaat caccaacggg tgcgcgtgta
    13321 tttgcgttaa cgacaaaagg tacaccagca catagcaatg taagctatca agatggagat
    13381 tatctccttt ttggccctga aacccgtggt ttgccacctt atgtgttaga taatatgcca
    13441 acacagcaga aaatcagagt tccaatgctt gctgatagtc gcagtatgaa cctgtcaaat
    13501 accgttgcag ttgtggtttt tgaagcatgg cgtcaacttg gttatcctgg tgcgttatta
    13561 agagactgaa atgcaaaaag cgctgatcat aaataatgag caagcgcttt tttaatttca
    13621 aatgactaaa aacttaattt attattacat tgagagttgt tttatttcag caacaacctt
    13681 cgtgagatac ctacaccctt cccctacatc cttcaatacc aatgcgtgag caccaatcac
    13741 tgcattatca ccaatctcta tctcaccgcc gataatcgaa gaattacaac caatataaac
    13801 gtggttgccg actcgaatat ctgctttacc atccttttct tttactccga tggtcacacc
    13861 ttgcattaac gtcacattct cacctaattg acagtaatta gtcagcacaa taccgatagg
    13921 atgtggtaat gaaaggcctt ttcctatgcg cgttcttaat gagatatcat tgcaatattt
    13981 acgattgttt ttagcaccaa tgtaaatgcc taattttttg agaaaacgat ttttagagaa
    14041 ataaaaaaat tgagcgacac gccaccaaaa cagataacgt gaacgttggc agaaaaagac
    14101 gcgatgaaaa agtcttatcc aactaaaagg cttgttatca ccgatatatt cagcgtgtaa
    14161 acaagcctta agttcagaaa atgtaacttc tgttaatgcc attaaatgcc atcaccacat
    14221 tcaaaaccag gtactacacc attaaagtgt tgatccatat cgagtgaagg tttatcactc
    14281 gtcggcttac caacaatgcg agcaggaacc cctgcaaccg tggtatgtgc aggcactgca
    14341 tgtaatacga cagatcccgc accaatttta gcaccgcgac caatctcaat attgccgagt
    14401 attttagcac ccgcaccaat catcacacct tcacgcactt taggatgacg atcaccacag
    14461 gttttacctg ttccgcctaa agtcacagat tgtaaaatag aaacatcatt ctcaacaaca
    14521 gctgtctcac caattacaat gcctgttgcg tgatcaagca taataccgtg gccaatacgt
    14581 gcagctgggt gaatatcgac accaaacgta acagaaatct ggttttgtag gtaaacagca
    14641 agcgctttgc gatcttgtcc ccataaccaa tgtgctatgc gataagcctg caacgcatga
    14701 aagcctttta aataaagcaa cggtgtggag tatttatcaa ctgcggggtc gcgtaaacga
    14761 acagctgaaa tatcataagc cgcagattcg atcatggaat catcgctacg atacgcttct
    14821 tcaacaatct cacgaacggc aatcgcaggc attgtctgtg tcgccagctt attcgctagc
    14881 atataactta aggcgctacc taagttttca tgtttgagta atgtcgcatg gaagaaactc
    14941 gccaaaatcg gctcgcaatc agccaaacat cttgcttcat ttttaatatg atcccagact
    15001 ctttttagtt cttctagcga catactcact ctcattcttt ttacattgaa acctaagtaa
    15061 caacttaagc ttcttatgtt gaaaaccgaa acagacacct taaaccaatg cgttaagtag
    15121 cgattattta aaatagactt agaacagagt gctgtctatt tcatcttttg ttgcgcgacc
    15181 taataaagat agtgccgcct ctttcgcgtc tttatgttgg tataaaattt ggtagatctg
    15241 ctcagtgata ggcatttcaa caccgacttg ttctgctaag gcgcgaactt ctttagtgtt
    15301 ccgataacct tcaaccacct gcccgatttt ctcttgggca gtatcaacat caagcccttg
    15361 gcctaacatc ataccaaatc ggcgattacg ggattggtta tcggtacaag ttaaaacgag
    15421 atcgcctaac ccggccattc ccataaacgt cgcagcatct gcacctaatg ctttacctaa
    15481 acggctcatt tcagcaagtc cgcgggtaat tagtgctgta cgtgcattcg cgccaaaccc
    15541 cataccatca gacattccag caccaatagc aatcacgttt tttaccgcgc cacctaattg
    15601 cacaccgata aagtcaggat ttttatacac acggaaactt ttaccacaat ggaacaactg
    15661 ttgaagctct tcagaaaacg tattatctgt tgatgcaaca gatatcgcag taggcagacc
    15721 tgctgcaagc tctttggcaa aagtaggacc agataaaact gcgagcggga tttcattacc
    15781 taatacttca cgcgccacat cttgtaacaa gcgaccagta tgagcctcaa gtccttttgt
    15841 cgcccaaaca acacgtgcat cctgacgtaa aaagggtttg atctgctgta atacgtcacc
    15901 aaaaacatgg cttgggatca caaccagaat attacggcta gcttcaatgg ctttttgcaa
    15961 agaagcttcc atgtataaac tatcaggaaa agaaacatcg ggcagaaatg cctgattaca
    16021 acgtgcttgt tctaacgccg caacatgttc agcatcatga ccccaaagta caacatcatg
    16081 gccattgcgt gctaaggtaa tcgctaaagc ggtgccgtat gaaccggcac cgataactgt
    16141 catagaagcg ttcattaagc ttcttcagcc ccttcattag cagcatcagt ttgttgctgt
    16201 tgtaaatagt tcataaacaa cgcatcaaag ttaactggtg ctaagttcag ttgtgggaaa
    16261 gtaccacggc tgactaagtt agtgatacat tcacgagcat aagggaacag aacgttaggg
    16321 caatatgcgc ctaagcaatg tgctaattga gtgccttcaa taccttcaac agtaaagata
    16381 cccgcttgtt gtacttcaca caggaatgca gtctcgttat ccatcgttgc agtcacagtg
    16441 actcgcaaaa taacttcata gacgttttca gctaaagtat tagaagatgt atccagatct
    16501 aatttaactt ctggttgcca ctctttttgg aaaacttgtg gagcattagg cgcttcaaaa
    16561 gagatatctt ttgtatagat acgttgaatt tgaaaagtca tctcttggtt ttgctgttct
    16621 gacat
//
