LOCUS       KY710715               13397 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 70/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710715
VERSION     KY710715.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 70/57"
                     /serotype="O44"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..13397
                     /note="O antigen gene cluster"
     gene            2..499
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             859..2049
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99768.1"
                     /translation="MKLNDIKNITILFPIILLSILSLYPYFIWGNSYIYKITLILSFI
                     SYIFILKKNDFLLKKNIVFSFILLLFWISYAFFPRGSVDISIPNTGVFAILLYCLLPA
                     VYRSNAFRYFRFVLSLILLISLIQYPFIVARYISPLNELIPFSEIKQERNQFYFNYIF
                     NIILNDQFLNFFNYSFFRFSSIFDEPGLLGTISAILLFTYQSNESKKLEKFIFLLSLV
                     LSFSLGGYLLFIIGVLIKLMLNFDIKRLILFFSLLTIFTSILANTDGGRRYITDRILN
                     TKQPITQTREDSSFSYKFNEKILSNGYYLTIGLGKDAHLKTGDDVSSYKGFIYNYGVF
                     GLTLLLSVLILIAYINDNINKQSVIILSLIFINFYQRPIEFNVYYYIIILSIIMKDKN
                     NNAV"
     CDS             2039..3235
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99769.1"
                     /translation="MQSKIITFLAITSKIINSFVILKILSIILSESDFSIFIYEFNKI
                     TVFQYIFTLGITTGIVAEAKKRSEIFGDVIKIINISLIILPCVLVFNIFLSISNPLII
                     LTSSSISLKLIIFSLLNGINEKKSFTILCTIDSIILLALIFVTKNSNNIDTVFLSYLL
                     SSLSVFFLSIFYLQKKFKLSEKTNYRSIIKKYKNYFLMSVISAFFVPLIYLITKKIIL
                     DNDAIDNKDIAILFSAWRLYDAFIMVIGTISSIHYIQMFNKNIHNKLNSLILKESFKI
                     TILIFLGILVSYLLFPQKIISLLLTNKYASEYHIFYILFVSCGFRAFSYVIGLYFVIF
                     QKVRIFIFIEVTSSLIMLLSSISLNMIGIIGFSLMFIFQSIFIIIISLIFITKNEKIR
                     LHYTNI"
     CDS             3201..4064
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99770.1"
                     /translation="MKKFAYIIPTYNRENNLIETVKSIINNCNSKNYEIIIVDDGSTD
                     NTQNKIKELKNTDIIKYIKKNNSGVSDSRNLGEQYSDSEWIIFIDSDDIIISDQLSLF
                     EKYIEKYPNAELLFTSYQYWNSNTQDVIPRKNLIFGYYNSFFDDFIQGIQPCFPGCVC
                     IKRNQFKNSLKFISKCNFGEDQALWVDLFYKLNCISIPDITLNYRIDSQNSLSKKKIQ
                     ILPPDISIAHSFGLHKYVAFRLSSFLLKSVKELNHILFINTLIYTIKSPSMIYPSLSF
                     TIKKLFKKLFK"
     CDS             4061..5188
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99771.1"
                     /translation="MNIIYICHEYPPFKTGGIGIYTKEIAENLSNKGHGVYVIGIYDQ
                     ENKSIEKINNVNIIRLPSSKNKILKKVINRCKLYVFLKIFIKKTKIDIIETPDFQGWL
                     AFFPKLKCKYLIRLHGSVTYFYELLKIKSYKKLIWKYIEKNALKKADKIISVSNFTAQ
                     ETKKIFNIKNSIDVIHNGIEINNKYIEKHPQNNMKKFVFAGSLIKKKGIIELVDAWKI
                     FSENKIDVELHIFGKDTEGLLSLFLIDESLKIGDNLFFHGAVEKNKLIEFYKSAHFFI
                     SPSKAEAFSLAPMEAMASSLLVIYNNQTSATELITHNENGYLLSNLNNLNIAKTLEIV
                     YALTDHEYNSITKASYLTVKDKFNVENLTNKNTSMYINLIK"
     CDS             5208..5981
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99772.1"
                     /translation="MPKFCIAISTMNERINNIIIPGYDKDVSYLLIHQINDENFYPNK
                     LLNERNDIIYINTNQFGLSKSRNMAINNCDAEYLFIMDDDVSYDIDAMKKIISLMSIE
                     NVDVGTFYHNYTNSNSTLKKIKKKITLNYINIAKVASIDICIKINSIKKNAIIFNENF
                     GLGTDLPSGEEMIFLSDCLKKNLNLIRYPLIVSTHPPIASGLDFYSSTNKIRAKKKML
                     EIIYGRFSFLVKIMFTIKKFKRASQAGFGFKFVKVMFLE"
     CDS             5978..7273
                     /note="orf07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf07"
                     /product="hypothetical protein"
                     /protein_id="AXY99773.1"
                     /translation="MINIKKIIYLIGEKSRNSSLRKIYKELKNSEFYSSEKLREIQFI
                     RMKELLIFSYENSVFYKKIFDEINFDPYKDFNSILDIKKIPIINKEQLIKFNYDIHTI
                     HNFKFKKTFFSETSGSSGQALTFYKDEEWDSYNRASIYRGLSWYDVKPYDKNGYFWGY
                     NLKFIKKIKTYFLDFLLNRYRLFKYDDKDITTFLKKSSNTIYLSGYSSMIYEVAKIAK
                     EKNIKLKKLKLIKGTSEKIYPHYNTETIAAFGLPIVSEYGSAESGIIAFECPYGNMHI
                     NEETCFVEEVEGEAIITNLIAKSFPTIRYNLGDYIKLSSTTCECGRSHRIIEEVIGRI
                     GKNILGYNNKKYPSLTLYYIFKNMALNYKIELSYRCEQKKIGELNIFLEKELDANSLE
                     KLNLEFKKYFGNNIYLNFFKVDSIKPKDKKLKDFESYID"
     CDS             7292..8458
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99774.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPSTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSRLVRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             8482..9234
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99775.1"
                     /translation="MSLNLSNDNALVSIIMPCFNSASFIQESINSVLSQTYKNFELII
                     IDDASKDNSLDIIKSFSDERIKIIELKTNQGAGLSRNKGIEAAQGRFIAFLDSDDLWH
                     KDKLSLQISFMKDNGYHLTYTHYQCFDKLGLKNIKHPPQSTNYDDLLYCNVIGCLTAI
                     YDAQLIGKQYMPLIRKRQDMGLWLKIMKDHGPAYCLPNILAYYRLDSGMTQNKANAAL
                     YQWIFYRQELKFNILKSTKYFIGYAYNGLIKK"
     CDS             9248..10198
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXY99776.1"
                     /translation="MITIIGGSGFIGTRLSDQLTKENIDFKIVDIAKSEKYPEKWIFG
                     DVTKPESLLEPLKNSSVIINLAAQHKDNVHPISLYYDVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWHKKDITNTLVVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNQKSMAYVENIASFLKFSTQLESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHSHIKVPYVIGLLGGYCFDILSKVTGKEFPVSSIR
                     IKKFCARTQFKSNHIAKTEFKAPVTLEQGIANTVKAEFSH"
     CDS             10202..10705
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99777.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(10778..11335)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXY99778.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGERCRYITKVVAEIRQPSE"
     CDS             complement(11335..12168)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99779.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVSGFECGDG
                     I"
     CDS             complement(12247..13263)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99780.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVSVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(13263..13397)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4806 a   2093 c   2041 g   4457 t
ORIGIN
        1 ttcacgcaac caacataaaa acgagttatt acgcgaaaca gtaaaagcca acgaattgtg
       61 ggatgacatt ttagataatg ccaaatttga ggctgaacag agtaataaga cgctacaagt
      121 aacaacacct ccgggagcat ggcctattta ctgcaatcca tattctctgg ctagtgcatt
      181 tgaaaacatt gttcgtaatg ccttacgtta ttcaaattct cgcattgagg ttgcctttac
      241 tgaacaaaac caaggtatca cgattattgt tgatgatgat ggcccgggtg ttagcccaga
      301 agatagagag catatcttcc gaccattcta tcgcactgat gaagcaagag atcgtgaatc
      361 aggtggtaca ggcttaggat tagctattgt agaaacagcc gtaagccaac atcgaggtca
      421 tgttaaagct gatgacagcc cattaggtgg gttgagggtt gagatttggt tgccgagagc
      481 gggtgcaagt aataaataac caacaagtta atagagtaat taaactatcg tatcaaaata
      541 tcatgccatc tattgaagaa taggtggcat attctttttg ttagcgccta acgatgtaat
      601 tcagaatgat ctggctgaat cgaccgctat tccagtattt atatatcctc aatcaatgct
      661 aaaataccca gctatcactt gaccgtctgg tttgaagagt ctactctcag tgcggttttt
      721 agcatattga taaagtatgc ttatataata aaactcttat attacatata gataagggtt
      781 tttcgagatg aactttggcg ctctgggggc cgtaaaccca gtgcggtagc tttttttata
      841 gcattttgat acctttttat gaaattgaat gatattaaaa atattactat attatttccg
      901 attatacttc taagcatttt atccttatat ccttatttta tatggggaaa tagttatata
      961 tataaaataa cattaatact ttcttttatt agttatattt ttattctaaa aaaaaatgat
     1021 tttcttttaa aaaaaaatat tgttttttca tttattttat tacttttttg gatatcctat
     1081 gctttttttc ctagaggaag tgtagatatc tctataccaa acactggagt ttttgcaata
     1141 ttattatatt gcctattgcc agcagtgtat agaagcaatg cgtttaggta tttcaggttt
     1201 gtcttatcat taatattact tatttcatta atacaatatc catttatagt tgcaagatat
     1261 atatcaccac tgaatgaatt aattccattt agtgaaataa aacaagaacg aaaccaattt
     1321 tattttaact atatttttaa tatcatatta aatgatcagt ttttaaattt ttttaattat
     1381 agctttttta gattttcatc tatttttgat gaaccgggtt tattaggaac aatatctgcc
     1441 attcttctat ttacctatca aagtaatgaa agtaaaaaat tagagaaatt tatattcttg
     1501 ctttctctag tattatcttt ttcactaggt gggtatctat tatttattat aggtgtttta
     1561 attaaactga tgctcaactt tgatattaaa agattaatac tgttttttag tctacttaca
     1621 attttcacca gtatacttgc aaacacagat gggggcagaa gatatattac agatagaatc
     1681 ctaaatacaa aacaaccaat cactcaaact agagaagatt cttcatttag ctataaattt
     1741 aatgagaaaa tactaagtaa tggatattat cttactatag gtttaggtaa agatgcacat
     1801 ttaaaaactg gagatgatgt ttcttcatat aaaggcttta tatataacta tggtgtattt
     1861 ggattaacac ttctattatc agtactaata ttaatagcat atataaatga caatataaat
     1921 aaacagtctg ttattatatt atctttaatt tttattaatt tctatcagag gcccatagaa
     1981 ttcaatgttt actactatat aataatactg tcaataataa tgaaagataa aaataacaat
     2041 gcagtctaaa attattactt ttcttgccat tacatctaaa ataataaact catttgttat
     2101 tttaaaaata ttatcaataa tattatcaga aagtgatttt tctattttta tttatgaatt
     2161 taataaaatt actgtatttc aatatatctt tactctaggt atcacaactg ggatagtagc
     2221 tgaagcaaaa aaaagaagtg agatatttgg tgatgtaatt aaaatcatta atatctcatt
     2281 aattatacta ccatgtgtat tagtatttaa tatattttta tcaatctcta atcctctaat
     2341 aatacttacc tcatcatcaa tttcattaaa attaatcata ttttcattac ttaatggtat
     2401 taatgagaaa aaaagtttca ctatattatg tactatagac tctattattc tattagcatt
     2461 aatttttgta acgaaaaatt caaataacat tgatacagta tttttatctt atcttctttc
     2521 atcattatct gtattttttt tatctatatt ttatttacaa aaaaaattca aattatcaga
     2581 gaagacaaat tatcgctcta taattaaaaa gtataagaat tattttttaa tgtctgttat
     2641 ttcagccttc tttgtaccat taatttattt aataactaaa aaaataatac ttgataatga
     2701 tgctattgat aataaagata ttgctatatt attctcagca tggcgcttat atgatgcatt
     2761 tattatggta ataggaacaa ttagctcaat acactacatt caaatgttca ataaaaacat
     2821 acataataag ctaaattcat taatacttaa agaatcattt aaaataacaa tactaatttt
     2881 tctcggtata ttagtttctt atttattatt tccacaaaaa ataatttctc tgttgcttac
     2941 aaataaatat gctagcgaat atcatatatt ttatatatta tttgtttcat gtggatttag
     3001 agcattttct tatgtaattg gattatactt tgttattttt caaaaagtaa ggatttttat
     3061 atttatagag gtaacatcta gcctaataat gttactcagt agtatttcgt taaacatgat
     3121 tggtattata gggtttagcc ttatgtttat atttcaatct atttttatta ttataatttc
     3181 acttatcttt attactaaaa atgaaaaaat tcgcttacat tataccaaca tataatagag
     3241 aaaataatct tatagaaacg gttaaatcca tcattaataa ttgtaatagt aagaattatg
     3301 aaataattat tgttgatgat ggtagtactg ataatactca gaataaaata aaggaattaa
     3361 agaatacaga cattataaaa tatataaaaa aaaataattc tggagtatct gactcaagaa
     3421 atttaggaga gcaatattct gatagtgaat ggataatatt tattgactct gatgatatta
     3481 tcattagtga tcaattaagc ctatttgaaa agtatattga aaaatatcct aatgctgaac
     3541 tcttatttac atcttatcag tattggaata gtaatacgca ggacgtaatt cctaggaaaa
     3601 atttaatatt cggatactac aattcctttt ttgatgattt cattcaaggg atacaacctt
     3661 gttttccagg gtgtgtatgt ataaaaagaa atcaatttaa aaactcatta aaatttatat
     3721 ctaaatgtaa ttttggtgag gatcaggcct tatgggtgga tttattttat aaattaaatt
     3781 gtatctctat acctgatata actttaaatt atagaataga cagtcaaaat tctttatcaa
     3841 aaaagaaaat acaaatttta cctccagata tatcaatagc gcatagtttt ggtttacata
     3901 agtatgtagc atttaggctt agctcatttt tattgaagtc agtaaaagag ttaaatcata
     3961 tattatttat taatacatta atttacacca taaaatcacc tagtatgata tatccttctc
     4021 tatctttcac aataaaaaaa ctttttaaga aattatttaa atgaatataa tatatatctg
     4081 tcatgaatat ccccctttca aaactggggg gataggaatt tatactaaag aaatagctga
     4141 aaacctatct aataaaggtc atggtgttta tgttataggg atttatgacc aagaaaataa
     4201 atcaatagaa aaaataaata atgtaaatat tattcgctta ccatcaagta aaaataaaat
     4261 attaaaaaaa gtaataaata gatgtaagct atatgttttt cttaaaattt tcataaaaaa
     4321 aacaaaaatt gatattattg agacccctga ctttcaagga tggttagctt tttttccaaa
     4381 actaaaatgt aaatatttaa taaggctaca tggttctgta acttactttt acgaactatt
     4441 aaaaataaaa tcatataaaa aattaatttg gaaatacatt gaaaaaaatg cattaaaaaa
     4501 agcagataaa ataatttcag ttagtaactt tacggctcaa gaaacaaaaa aaatatttaa
     4561 tataaaaaat agcattgatg ttattcataa tggaattgaa ataaataata aatacattga
     4621 aaagcatcca caaaataata tgaaaaaatt tgtttttgca ggctcattaa ttaaaaagaa
     4681 aggaattatt gaactagttg atgcatggaa aatattttca gaaaataaaa tagatgttga
     4741 attacatatc tttggtaagg atacagaagg attattaagc ttatttttaa ttgatgaaag
     4801 cctcaagatt ggtgataatc tattttttca tggcgccgta gaaaaaaata aacttattga
     4861 attttataaa agtgctcatt tttttatatc tccatctaag gcggaagcgt tttcattagc
     4921 cccaatggaa gccatggcaa gttctcttct tgtcatttat aataatcaaa ctagtgctac
     4981 tgagctcata actcacaatg agaacggtta tttattaagc aatttaaaca atctaaatat
     5041 tgctaaaact ttagaaattg tatatgcact aactgaccat gaatacaatt caattacaaa
     5101 agcatcttac ttaacagtaa aagacaaatt taatgttgaa aatttaacaa ataaaaatac
     5161 ttcaatgtat attaacttga ttaagtaaat ttaaaaaaga gtaagacatg cctaaattct
     5221 gtattgccat atcaacgatg aatgagagaa ttaataatat tatcattcct ggatatgata
     5281 aagatgttag ttatctttta atacatcaaa taaatgatga aaatttttat ccaaacaagc
     5341 tcttaaatga aagaaacgat attatatata ttaatactaa tcaattcggc ttatcgaaaa
     5401 gtagaaatat ggcaattaat aattgtgatg cagaatatct atttattatg gatgatgatg
     5461 tttcttatga tattgatgcg atgaaaaaaa taatatctct tatgtcaatt gaaaatgttg
     5521 atgttggtac attttatcat aattatacaa atagtaactc aaccttaaaa aaaatcaaaa
     5581 aaaaaatcac acttaattat ataaatattg ccaaagtagc gtctatcgat atatgtatta
     5641 agattaatag tattaagaaa aacgctatca tttttaatga aaattttggc ttaggtaccg
     5701 accttccatc aggtgaagaa atgatatttt tatctgattg tttaaagaag aatctcaacc
     5761 taattagata tcctctgatt gtatcaactc atccaccgat agcatctgga ctagacttct
     5821 attcatccac caataaaatt agagcaaaaa agaagatgct tgaaattata tatggcaggt
     5881 tctctttttt agtcaaaata atgtttacta taaaaaaatt caaaagagca tctcaagctg
     5941 gttttggatt taaatttgta aaggtaatgt ttttagaatg ataaatataa aaaaaataat
     6001 atatttaata ggtgaaaaat ctagaaatag ctctttacgt aaaatctata aagagttaaa
     6061 aaactccgag ttttattctt cagaaaaatt aagagaaatt caatttatcc gaatgaaaga
     6121 gttattgata ttttcttatg aaaatagtgt tttttataaa aaaatttttg atgaaataaa
     6181 tttcgatccg tataaagatt ttaactctat tttagacatc aaaaaaatac ctattataaa
     6241 taaagaacag cttattaaat tcaattatga catacatact atacataatt ttaaatttaa
     6301 gaaaactttc ttttctgaaa catccggatc atcaggacaa gcattaacat tttataagga
     6361 tgaagaatgg gactcctaca atagagcatc aatttataga gggctaagtt ggtatgatgt
     6421 taaaccttat gataaaaatg gatatttttg ggggtataat ctaaaattta taaaaaaaat
     6481 aaaaacctac tttcttgatt ttcttttaaa tagatataga ctatttaaat atgatgataa
     6541 agatattact acttttttga aaaaaagtag taatactatt tatttaagtg gctatagttc
     6601 aatgatttat gaagtagcta aaatagcaaa agaaaaaaac ataaagctaa aaaaacttaa
     6661 attaataaaa ggtacttctg aaaaaatata tcctcactat aatacagaaa ctattgcagc
     6721 tttcggatta cctattgtaa gtgaatatgg ttctgccgaa tctgggatta ttgcgtttga
     6781 atgtccttat ggaaatatgc atataaatga ggaaacttgt tttgtagaag aagttgaagg
     6841 tgaagcaatc attacaaatc taatagcaaa atcatttcca actattcgat ataatctagg
     6901 tgattatata aaattatcct ccaccacatg cgaatgtgga cgttcccatc gcattataga
     6961 agaggttata ggaagaatag gaaaaaatat tttaggatat aataataaga aatacccatc
     7021 tttaacctta tattatattt ttaaaaacat ggcattaaat tataaaatag aattatctta
     7081 tcgatgtgag cagaaaaaaa taggtgaatt aaatatattt ttagaaaaag aactagatgc
     7141 caattcttta gaaaaattaa atttagaatt taaaaaatat tttggtaaca atatttacct
     7201 taattttttt aaagttgact caataaagcc taaagataag aaactaaaag attttgaaag
     7261 ttatattgat taattttatt aggataatta aatgaaaatc acaatctccg gtactggcta
     7321 tgtcggtcta tctaacggga tacttctttc ccaaaacaac aaagttgttg cgttagatat
     7381 caataaagaa aaagttgaat tacttaacaa aaaaatctct ccaattacag ataaagaaat
     7441 tgaagagttt ttagcaacca aagagctcga ctttacggct acattagata aagaattcgc
     7501 ttataaagat gctgatttcg ttatcattgc aacgcctacc gattatgatc ctaaaacaaa
     7561 ttactttaac acatcttctg tagaatctgt tatccgtgat gttttagaat ataatccaag
     7621 cactacaatg attataaaat caaccattcc tgttggtttt acaaaggaaa tgagagaaaa
     7681 atataataca gataatatta ttttctcacc tgaattctta cgtgaaggtc gtgctcttta
     7741 cgataactta tacccttctc gtattgtgat tggcgagcaa tcagaaagag cacaaaagtt
     7801 tgcagacctt ttacagcaag gtgcactaaa gaaagatatt gatgttctgt tcactgactc
     7861 tacagaagca gaagccatta aattatttgc taatacttat ttagcattgc gtgttgctta
     7921 ctttaatgaa ttagatagct atgctcaagc ttatggctta aatgcaaaac aaatcattga
     7981 aggcgtctgt ttagatccac gtattggtaa tcactataat aacccatcat tcggttacgg
     8041 cggttactgt ttaccaaaag acaccaaaca actgttagca aactacgact cggtgcctaa
     8101 taatattatt agtgccatcg ttgaagcaaa ccgcacacgt aaagacttta ttgccgactc
     8161 aattatcgct aaatcaccaa aaatcgtagg tgtttatcgt ttaattatga aatctggttc
     8221 tgataatttc cgctcttctt ctatccaagg cattatgaaa cgcattaaag ccaaaggtat
     8281 tgaagtggtg atttatgaac ctgaaatgaa agaagaaacc ttctttaact caagattagt
     8341 tcgtgattta aatgagttta aagctatgtc tgatgtcatt attaccaata gaatgtcatc
     8401 agcattaaac gatgttgaag aaaaagttta tactcgtgac ctctttggca atgattaatt
     8461 atatagattt aggtatctcc tatgtcattg aatctgtcta atgataatgc tcttgtttct
     8521 attataatgc cttgttttaa ttcagctagc tttatacagg aatctattaa ttctgtctta
     8581 tctcaaactt ataaaaattt tgaattaata attatagatg atgcatctaa agataatagc
     8641 ctagatatta ttaagtcatt tagtgatgag agaataaaaa ttatagagtt aaagacaaat
     8701 caaggtgcag gtctttctag aaataaagga attgaggctg ctcaaggaag atttattgct
     8761 tttttagata gcgatgattt atggcacaaa gataaattat cattacaaat aagttttatg
     8821 aaagataatg ggtatcatct tacatatact cattatcaat gttttgacaa attaggttta
     8881 aaaaatataa aacatccccc acaatcaaca aattacgatg atttattata ttgtaatgtt
     8941 attggatgtc tgactgctat ttatgatgcc caattaattg gtaaacaata catgccatta
     9001 attagaaaaa ggcaagatat gggattatgg ctaaaaatca tgaaagatca cggtccagct
     9061 tattgcctac caaatatctt agcatattac cgacttgact ctggtatgac tcaaaataaa
     9121 gctaatgcag ctttatacca atggatattt tatagacaag aattaaagtt taatatatta
     9181 aaatcaacta aatattttat aggctatgct tataacggtt tgataaaaaa ataaggcagt
     9241 atttaatatg attacaatta ttggtggttc aggttttatt ggtactcgtc tttcagacca
     9301 attaactaaa gaaaatattg attttaaaat tgtggatatt gccaaaagtg aaaagtatcc
     9361 tgaaaaatgg atattcggtg atgtaacaaa acctgagtca ttattggaac cattaaaaaa
     9421 ctctagtgtg attattaatt tagctgcaca acataaagat aatgttcatc ctattagtct
     9481 atattatgat gtcaatgtgg atggtgctaa aaatgtttgt gatgttgctg aacaactaaa
     9541 tattaaacat atcgtattta cttcatctgt tgctgtatat ggatttgttg aaaaagaaac
     9601 tggcgaagac ggtgagttcc atccatttaa cgattatgga aagtcaaagc ttgaagctga
     9661 acataaatat gaagcctggc ataaaaaaga tattacaaat actctagttg ttatcagacc
     9721 aactgttgta ttcggtgaag gaaaccgagg taatgtctat aatttattcc gtcaaatagc
     9781 atccggtaag tttctaatgg ttggctctgg taacaaccaa aaatcaatgg cttacgtgga
     9841 aaatattgct tcattcttaa aattttctac tcaactagaa tctgggcacc atatatttaa
     9901 ttatattgat aaacctgact ttacaatgaa tgagctcaca gacattatct atacagcttt
     9961 agataaacgt catagtcata ttaaagtacc ttatgttatc ggattacttg gtggatattg
    10021 ctttgatatt ctgtctaaag tgacaggtaa agaatttcct gttagtagta ttcgtattaa
    10081 gaaattctgt gctcgtaccc agtttaaatc gaatcatatt gctaaaactg aatttaaagc
    10141 accagttact ctggaacaag gcatagcaaa tacagttaaa gcagaattta gccattaaac
    10201 catgctcaat atcgtcttat tcgaacccga aattccaccg aatacgggta atattatccg
    10261 cctgtgtgct aacacaggct ttcatcttca tttgatccaa ccattgggct ttacttggga
    10321 tgataaacgt ctgcgtcgtg cagggcttga ttatcgtgaa tttgctgata ttaaacagca
    10381 ccacgattac tatgcttttt tagaaagtga aggtttaaac cctgataatg ctcaatcgcc
    10441 aactggcgca cgtgtatttg cgttaacgac aaaaggcaca ccagcacata gtaatgtgag
    10501 ttatcaagat ggagattatc tcctttttgg acctgaaacc cgtggtttac cgccttatgt
    10561 gttagacaat atgccaacac agcagaaaat cagagttcca atgcttgctg atagccgtag
    10621 tatgaacctg tcaaataccg ttgccgttgt ggtttttgaa gcatggcgtc aacttggtta
    10681 tcctggtgca ttactaagag actgaaatgt aaaaagcgct gatcataaat aatgactagc
    10741 gcttttttta atttcaaacg actaaaaatc taacttatta ctctgatggt tgtctgattt
    10801 cggcgacaac tttagtaata taccgacacc tttcccccac atcctttaat accaatgcgt
    10861 gagcaccaat caccgcatta tcaccaatct ctatctctcc gccgataatc gaagaattac
    10921 aaccaatata aacatggttg ccaactcgaa tatctgcttt gccatccttc tcttttactc
    10981 caatggtgac accttgcatt aacgtcacat tctcacctaa ctggcagtaa ttagtcagta
    11041 cgatccctat cggatgaggt aatgaaagac ctttgcctat gcgagttctt agtgaaatat
    11101 cgttgcaata cttacgatta tttttagcac caatacgaat gcctaatttt ttaagaaaac
    11161 gatttttaga aaaataaaag aattgggcga cacgccacca aaatagataa cgtgaacgtt
    11221 gacagaaaaa gacgcgatga aatagtctta tccaactaaa aggcttgtta tcaccaatat
    11281 actcagcatg taaacaagcc ttaagttcag agagcgtaac ttcagttaat gccattaaat
    11341 gccatcacca cattcaaaac cagacaccac accattaaag tgttgatcca tatcgagtga
    11401 aggcttgtca ctcgttggct taccgacaat acgggcagga acaccagcaa ctgtagtgtg
    11461 tgcaggaact tcatgtaata ccacagagcc agcaccaatt ttggcaccgc gaccaatctc
    11521 aatattaccg agtatttttg cacccgcccc aatcatcaca ccttcacgta ctttaggatg
    11581 acgatcacca caagttttac ctgttccacc taaagtcacg gattgtaaaa tagaaacatc
    11641 gttctctacg atagccgttt caccaatgac aatgcccgtt gcgtgatcaa gcataatacc
    11701 gtgaccaata cgtgcagcag ggtgaatatc gacaccaaaa gtcacagaga tttggttttg
    11761 cagataaacg gcaagcgctt tacgatcttg gttccataac caatgggcta tacgataagc
    11821 ttgtagcgca tgaaaccctt ttaaataaag tagtggcgta gagtatttat caactgcagg
    11881 gtcgcgtaaa cgaacggctg aaatatcata agccgctgat tcaatcatag aattatcgct
    11941 acgatatgct tcttcaacaa tctcacgaac agcgatcgca ggcatcgtct gggttgccag
    12001 cttattcgcc aacatataac ttaaggcgct acctaagttt tcatgtttga gtaatgtcgc
    12061 atggaagaaa ctcgccaaaa ttggctcaca atctgccaaa catcttgctt catttttaat
    12121 atgatcccag actcttttta gctcttctag cgacatactc actctcattc tttttacatt
    12181 gaagcctaag taaaaactta ggctttttat gtttaaaaca gaaataaata actatctaaa
    12241 atagacttag aaaagattgc tgtctatctc atctttagtg gctcggccta ataaagataa
    12301 tgcggcttct ttcgcgtctt tatgctgata taaaatttgg tagatctgct ctgtaatagg
    12361 catttcaaca ctgacttgtt ctgctaatgc gcgaacttct ttggtattgc gataaccttc
    12421 aacgacttgc ccaatttttt cttgggcggt atcaacatca aaaccttggc ctagcatcat
    12481 accaaatcga cgattacgag attggttatc ggtacaggtt aaaactaaat ctcccaatcc
    12541 tgccattccc ataaacgttg cggcatctgc acctaatgct ttacctaaac ggctcatttc
    12601 agcaaggcca cgagtgatta gtgctgtacg tgcattcgca ccaaacccca taccatcaga
    12661 catacctgcg ccaatcgcaa tcacgttttt taccgcgcca cctaattgca caccgataaa
    12721 gtcaggattt ttatacacac ggaaactttt gccacaatgg aacaactgct gtaactcttc
    12781 agagaaggta ttgtccgttg atgcaactga aatcgcagta ggcagacctg ctgcaagctc
    12841 tttagcaaaa gtagggcctg ataaaactgc tagcgggatt tcattaccta atacttcacg
    12901 cgctacatct tgcaacaagc gaccagtatg agcctcaagt ccttttgtcg cccaaacaac
    12961 acgtgcatcc tgacgtaaaa agggtttgat ctgttgtaat acatcaccaa aaacatggct
    13021 tgggatcaca acaagaatat tacggctggc ttcaatggct ttttgcaaag aagcttccat
    13081 gtataaacta tcaggaaagg aaacatcggg cagaaatgcc tgattacagc gtgcttgctc
    13141 taatgcagca acatgctcag gatcatgacc ccaaagtaca acatcatggc cattgcgcgc
    13201 taaggtaatc gctaaagcgg tgccgtatga accggcaccg ataactgtca tagaagcgtt
    13261 cattaagctt cttcagcccc ttcattagcg gcatcagttt gttgctgttg taaatagttc
    13321 ataaacaacg catcaaagtt aactggtgct aagttcagtt gtgggaaagt accacggctg
    13381 actaagttag tgataca
//
