LOCUS       KY710717               13400 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 74/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710717
VERSION     KY710717.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 74/57"
                     /serotype="O48"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..1401
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..13400
                     /note="O antigen gene cluster"
     CDS             1741..3009
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99799.1"
                     /translation="MLDINNWFAGYHILSYKNINKNIFYLIFVQLANYIAPLVILPYL
                     SRTIGIDGVGTVVIALSLCSLALIFTDFGFAVAGPYWIAKNYNKKNKLALYMGSIFIL
                     KLIFFIIFLFVYIIFSNFISFLKDQTVYFHIIILSTIFFQAFQITWFFQGIEKMKNIT
                     ICTVTTKITYLILVIIFVNNKGDINFTLFCFMFSNLLSATLGLYLMYKEKYYILISTP
                     KYIWRIFKENTSFFLSRAAVSIYTSASTFLVGNYAGIQQAALYSSAEKLYQGAISLTA
                     PVSQALYPYLAKSGDRKVLYKFIFILSPIMLFCGLICIYFSNSIIIIIYGEDFLEASS
                     LLKVFILISIISFISINFGYPAYSTIGRLDLVNKSVILGGILQLSLIMVLIFTDRLNA
                     NSIVISILFTEIIILLFRVLNYLNIIRKES"
     CDS             3012..4172
                     /note="CDP-glycerol:poly(glycerophosphate)"
                     /codon_start=1
                     /transl_table=11
                     /gene="tagF"
                     /product="tagF"
                     /protein_id="AXY99800.1"
                     /translation="MNTFFRLIISFIDIFIPKNKRKISIPCINDNEWQGNCSFLFQYL
                     IKNKESLGIQINILCNKKSTLLRNQDTRLNTQYLYSFKGIWTLLTSGIVIYHHGPLAG
                     RIPLISSHRINFHINHGIHFKKIELALDSKSQEYKNISFIKTKWLCKYHSVSSNIDAL
                     SCCSDYHTYLSNIFITGIPRNDLFFINNDDLLPQEVINDINKLKNLAKNRKIILYAPT
                     WRNQGGAYIFDQDEKYSLEQFLINNDCIFIYAGHPYLKNRVIPKSKYIIDYNNNFNDI
                     QSVLSITDILITDYSSIWIDFLLKDKPIIGFQFDHNTYEDERGFLFDYNEVFPGEIVY
                     DFQSLILALKHNINVDFIKTEKFLFSKKIFHKFTDGKNCERIANSLLSIKND"
     CDS             4165..5337
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99801.1"
                     /translation="MIKYKKYNPILNKYLIILLLLITNMQTTSAFSLYFLCIFVFGLI
                     ATLIVVTNHTIKLSLLISIIVLILTLGILTNFHADRYVNYIGLNDILYKKYILVIFWS
                     LMSLLSINIFIHTPKSIIKKSLSIVILIMSFFVILQFFSYYALNITIDFSLITGGIES
                     RSYFGNYRPSGFLPEPAVYSGHMSGLLSLYLFYNKKIDRYFILGMTSIFITQSTLGII
                     LISLILITYLLTSKIKKNKLFFTSIFLALIFLFIVPEIVNRIDVFTQGADTSNNLKLL
                     AIMNFIQDDNIFSFGYGLVSKDHDYLPSYYEATKDITLFGSILSIYGVFLGTIILLVF
                     IFILLKSKIQVKYKLILTIPLLKLCSPGYAFFFIYILWFFIILNEKKFNTEHQSQS"
     CDS             5334..6443
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99802.1"
                     /translation="MKILLIITGLGIGGAETQLCDLADNFLFEENNEIIIVSLSKHKI
                     LKKPKNKKIKIVSLNIEKTPISFLVGFIKAIKLVKRFKPDIIHSHMYHANIFSRFLRI
                     FCKIPVLISTAHSSDEGGKLRMFLYRITDRLASISTNVSQEAVESFIEKNATTKNRMI
                     AIYNGIDTNKFHYSKQVREKIRNQLGFSDNDIILLSVGRLTKAKDYPNLFHAFSALPN
                     RENIYLLIIGTGEEENNLRNLANHLNITKNIIWIGLKYNVQDWLSACDIFILSSQWEG
                     FGLVVAEAMATERVVIGTDCGGIKEVIGNYGFIIPKKDSTALKNSINKVLQLTDRERT
                     NLGFNARNHIENNFSLNMISKQWLSLYSKLHDKKR"
     CDS             6440..7576
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99803.1"
                     /translation="MKIAIIGTTSASLLNFRKELIISLVSLGHIVYCFSIDYTKESKF
                     EVSQLGAIPIEYKLSRSGLNPFLDIKNTYILYKILKRLNIELTLSYFSKPAIFGTIAA
                     KLANTPQIYAMLEGLGYYFTEQKNGLSLKTKIVKYIQILLYKIAFPLSNGLILLNNDD
                     KKDLIERYKIKTEVFIWGGIGLDLNQYKKSDIIINDKISFLFIGRLLKEKGIFEYIHA
                     AEKIKKSYSNIEFIVLGDIDMDNPGSLTKKDLDNLIEKNIITYPGHVDNVIEWLNKSH
                     VFVLPSYREGLPRSTQEAMAIGRAIITTDVPGCRDTVSNKKNGLLVKPWSSDELASKM
                     EYLIKNKSEIIKMGNNSYSYAQKNFDQKITNKKILNYMKITNEK"
     CDS             7696..8862
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99804.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKKLDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQKGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             8874..9377
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99805.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVIFEAWRQLGY
                     PGALLRD"
     CDS             9484..10467
                     /note="orf09"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf09"
                     /product="hypothetical protein"
                     /protein_id="AXY99806.1"
                     /translation="MHNVTLSFKYSHHISQYIYGFLLLSKNNIIKLSNIDKDTNITGA
                     QHILRANIDGKKIIYDANDGDHIDRGFFSIPDYEWCDLYFKRSYSNQLAEQFPKCRPL
                     GFNYEIKPFYGMIDRFFGDIRRLMGKEIVKHTDLEIIPNVSNNPKILFLTRLWEPNPL
                     KDNASKIELEYYNETIQLNKVRMDALNMIHAQFNERSTIGINDIPYSRRMAPDFILPK
                     EKTHRRNFINMMKEHEICITSTGLHKSTGWRFGEFVAASRAIISEPLNYVVPGNFDNY
                     LPFNNIEELYLAIEKLVNDKELRYDMMKRNNIYYNNYLKPDRLILNTITSL"
     CDS             complement(10525..11079)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXY99807.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKEGKANIQVGNHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGERCRYITKVIADIRRV"
     CDS             complement(11082..11903)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99808.1"
                     /translation="MSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHENLGSA
                     LSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIMLDHAT
                     GIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDGI"
     CDS             complement(12015..13028)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99809.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(13028..13270)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4538 a   2268 c   2219 g   4375 t
ORIGIN
        1 tcttcacaca ggaaagcggt gggggggccg cccgcagggg gaaaaagtga ataccgtcaa
       61 ggtgttatgc ttgttgtact tcacgcagga aggcggcttg ttgtacttca cacaggaaag
      121 cggactgaaa gtgaataccg tcaaggtgtt atgctagagc aacatataga agccgaacta
      181 gcgcaagatc cggctaacga tctgctatgg tggcggcgat taatccgcgc cattgacaaa
      241 tgggcgccac ctggtcaacg tcttattatt gtcaccagtg aaggtcgtat tattggtgct
      301 caacgtaatg agatacaagt tgtcagaaac tttatggggc agtctgataa cgcagaccat
      361 ccgaaaaaga aaaaatacgg tcgctctgag atgttaggcc ccttttccat tagagatggt
      421 gaagatcact accaacttta cttagtgcgc ccatcaagta gcccgcaatc tgattttatc
      481 aatttattat ttgataaacc gcttttatta ctgatattca ctatgcttat cagtacaccg
      541 ctactggttt ggctctcttg gagcttggca aaaccggccc gaaaactcaa aaatgcggcg
      601 gatgatgtgg caaaaggtaa tctgcgccct caccctgaac tggaaaccgg cccgcaagaa
      661 tttttagcgg caggcaccag ttttaatcag atgatcagcg ccttagaacg catggttgaa
      721 gcgcaacagc gattgatttc tgatatctcc catgagttgc gcaccccatt aactcgttta
      781 cagcttgcca gtgcattact acgtcgtcgt agcggtgaaa gtaaagagct agagcgtatt
      841 gaaacagaaa cacagcggct agatggcatg atcaatgact tattagtgct ttcccgcaat
      901 caatataaaa atgagttatt acgcgaaacc gtaaaagcga atgagctttg gaacgatatt
      961 ttagataatg cgaaatttga ggctgaacag agcaataaaa ctctaacagt aaccgccccg
     1021 ccaggcccat ggacgattta ttgcaatcct tattcattag cgagtgcctt tgaaaatata
     1081 gttcgcaatg ccttgcgtta ttcacattct cgtattgaag tggcttttac cgaacaaaat
     1141 cagggtatca ctattattgt tgatgatgat ggccctgggg ttagcccaga agatcgcgag
     1201 catattttcc gaccttttta tcgtacggac gaagcacgtg atagagaatc cggcggtaca
     1261 ggactaggac tggcaattgt tgaaaccgca attagtcaac atcgaggcca tgtaaaagct
     1321 gacgatagcc cattaggggg gttaagagtg gagatttggt tgccgagagg gagtgtggta
     1381 aataaatcac aacacactta attcagtaat aaaattacta cattaaaata ttctgccatc
     1441 tattgaagaa taggtggcat attcttttca ttcccatctt tatagatatt tcagaatgat
     1501 cttgctaatt cagtcgatat tccgatattt atatatacct aatcaatgtt agaataccca
     1561 tcaattactt gacccgcggg tttgaagagt ctaatctcag agcggttttg cgtactgtta
     1621 aagtatgctt atataataaa actcttatat tacagacaga taagggtttt aaaagagaaa
     1681 ttttggcgct ctgggggccg taaacctagt gcggtagctt tttttgatta ttaatatcta
     1741 ttgttagata ttaataattg gtttgctgga tatcatattt tgtcttataa aaatatcaat
     1801 aaaaatattt tttatcttat ttttgtacag cttgctaatt atatagctcc acttgttata
     1861 ttaccttatt tatctagaac tataggtatt gacggtgttg gcactgttgt aatagcccta
     1921 tcactgtgtt cattagcatt gatatttaca gatttcggtt ttgcagtagc cggcccttat
     1981 tggatagcaa aaaattataa taaaaaaaat aaattagctt tatacatggg ttcaatattt
     2041 atattgaaat taattttttt cataatattt ttattcgtat atataatatt ctctaatttt
     2101 atttcttttt taaaagatca gacggtatac ttccatataa ttatcttatc tactatattt
     2161 tttcaagctt ttcaaataac ttggttcttt cagggaatcg agaaaatgaa aaatatcact
     2221 atttgtacag taacgacaaa aatcacatac cttatattag tgattatttt tgtcaataat
     2281 aaaggtgata ttaattttac tcttttttgt tttatgttta gtaatttatt gtctgctact
     2341 ttaggtcttt atttaatgta taaggaaaag tattatatac ttatatcaac ccctaaatat
     2401 atttggagaa tattcaaaga aaatacttct ttttttcttt ctcgagcagc agtcagtata
     2461 tataccagtg ctagtacttt tcttgtaggc aattatgctg gtatacagca agccgctcta
     2521 tattctagcg cagaaaaact ctatcaaggg gctattagtt taacagctcc tgtaagccaa
     2581 gctttatatc cttatttagc caaaagtggt gataggaaag tattatataa atttattttt
     2641 attctttctc ctataatgtt gttttgtgga ttaatttgta tttacttttc caactccata
     2701 ataattatta tttatggcga agattttcta gaagcatcta gcttattaaa agtatttatt
     2761 ttaatttcta taattagttt tattagtatc aattttggtt accctgccta ttcaacaatt
     2821 gggcggcttg atttagtaaa taaaagcgtc atattaggcg gaattttgca attgtcatta
     2881 attatggtgt taatttttac agatagactt aatgctaact caattgttat ttcaatcctc
     2941 ttcactgaaa tcatcattct attatttaga gtattaaact atttaaacat tataagaaaa
     3001 gagagttaac tatgaatact ttttttagat taataatttc atttatagac attttcattc
     3061 ctaagaataa aagaaaaata tctataccat gcataaatga taatgagtgg caggggaact
     3121 gctcattcct ttttcaatac cttattaaaa ataaagaatc attaggtatt caaataaata
     3181 tactttgcaa taaaaaaagc acgttgctca ggaatcaaga tactcgttta aatacacaat
     3241 acttatattc attcaaagga atatggacat tattaacatc aggtattgtt atttatcatc
     3301 atggtccatt ggcaggtaga attccactaa tatcatctca tagaataaat tttcatatta
     3361 accatggcat acactttaaa aaaatagaat tagctttaga ttctaaatct caagaatata
     3421 aaaatatatc ttttattaaa acaaaatggc tttgtaaata tcattctgtt tcatcaaaca
     3481 tagatgcact atcctgttgt tctgattatc atacttattt gtcaaatatt tttataactg
     3541 gtattccaag aaatgattta ttttttataa ataatgatga tttactgcca caagaagtta
     3601 ttaacgatat caataaacta aaaaacttag ctaaaaatag aaaaattatt ctttatgctc
     3661 cgacttggag aaatcaagga ggagcataca tttttgatca agatgaaaaa tatagtctag
     3721 aacagttctt aataaacaat gattgtatct ttatatatgc aggacaccct tacttaaaaa
     3781 atagagttat tccaaagtct aaatatataa ttgattataa taataatttt aatgacatcc
     3841 aatctgtact tagtattact gatattttaa tcactgatta ttctagcata tggatcgatt
     3901 ttttactaaa agataaacct atcattggtt tccaatttga ccataacaca tatgaagatg
     3961 aacgaggttt tttattcgat tataatgaag ttttccctgg agaaattgtt tatgattttc
     4021 agtcactaat tttagcatta aagcataata ttaatgtgga ttttataaaa acagaaaaat
     4081 ttcttttttc taaaaaaata tttcataaat tcactgatgg aaaaaattgt gaaagaattg
     4141 caaattcact attaagtata aaaaatgatt aaatataaaa aatataatcc aatattaaat
     4201 aagtacttga taattttatt attacttatt actaatatgc aaacaacaag tgctttttct
     4261 ctatattttc tatgtatatt tgtattcgga ctaattgcta cgttaatagt tgtaactaac
     4321 catactataa aactaagtct ccttatatct attatagtat taatactcac attaggaata
     4381 ctgactaatt ttcacgcaga tagatatgtt aattatattg gattaaatga tattttatat
     4441 aaaaaatata ttttagtcat tttttggagc ttaatgtcat tattatctat taatatcttt
     4501 atacataccc caaagagtat aataaaaaaa tcattgtcaa tagtcatatt aataatgagt
     4561 ttttttgtta ttcttcaatt tttctcttat tatgccttaa atattactat tgacttttct
     4621 ttaataactg gaggtataga atctagaagc tattttggga attatcggcc tagtggtttt
     4681 ttgcctgaac ctgcggttta cagtggacat atgagtggtt tactatcttt atacttattt
     4741 tataataaaa aaattgatag gtattttatt ttaggtatga catcaatatt tattactcaa
     4801 tcaactctag gtataattct aataagcctt attcttatta cttatttatt aacatcaaaa
     4861 ataaaaaaaa ataaattatt ttttacttct atttttttag cattaatttt tttatttata
     4921 gttcctgaaa ttgttaatag aattgatgtt ttcacacaag gtgcagatac tagcaataac
     4981 ttaaaactac ttgctattat gaatttcatt caagatgata atatattttc atttgggtat
     5041 ggtttagtat caaaagatca tgactattta ccttcatatt atgaagcaac caaagatatt
     5101 acattatttg gctcaatact ttctatttat ggagtatttt taggcaccat aatattatta
     5161 gttttcatat tcatattatt aaaatcaaag attcaagtaa aatataaact aatattaacc
     5221 atacctcttt taaaactatg tagccctggg tatgcttttt tctttatata tatcttatgg
     5281 ttttttataa ttttgaatga aaaaaaattc aatacagagc atcagagtca atcatgaaaa
     5341 tcttattaat tataactggc cttggtatag gtggggcaga aactcaatta tgtgatttag
     5401 ccgataactt cttgtttgaa gaaaacaatg aaattatcat cgtttcttta tctaaacata
     5461 aaatactaaa aaaaccaaaa aataaaaaaa taaaaatagt aagtttaaat atagaaaaaa
     5521 cacctatctc ttttcttgta ggatttatta aagcaataaa attagtaaag agattcaaac
     5581 ccgatattat tcatagtcat atgtatcatg ctaatatttt ttctcgcttt ttacgtatat
     5641 tttgtaaaat accggtcctc atatcaacag cccatagttc tgatgaagga ggaaagttgc
     5701 gaatgtttct atacaggatt actgatagat tagcatcaat ttcgactaat gtaagtcaag
     5761 aagctgttga atcatttata gaaaaaaatg ctacaactaa aaatagaatg atagctattt
     5821 ataatggcat tgatacaaat aagtttcatt actctaaaca agtaagagaa aaaatacgaa
     5881 atcaattagg atttagcgat aatgatatta tattactttc ggtaggtaga ctaacgaaag
     5941 caaaagacta ccctaatttg tttcatgcat tcagtgcact tcctaacaga gaaaatattt
     6001 atttattaat aataggtact ggagaagaag aaaataattt gagaaattta gctaatcatc
     6061 taaatattac caaaaatata atttggatag gattaaagta taatgttcaa gattggcttt
     6121 cagcctgtga tatttttata ttatcatctc aatgggaagg ctttggttta gttgtagctg
     6181 aagctatggc tacggaaaga gtcgtaattg gcactgattg tggtggaata aaggaagtta
     6241 ttggaaatta tggatttatc attcctaaaa aagatagtac ggcattaaaa aattcaataa
     6301 ataaagtgtt acaattgact gatagagaaa gaacaaactt agggtttaat gctagaaatc
     6361 atatcgaaaa taacttttca ttaaacatga tatctaaaca atggctatca ctctatagta
     6421 aactacacga taaaaaaaga tgaaaattgc aattattggt accacttcgg cttcattact
     6481 aaactttaga aaagagttaa ttattagcct tgtatcctta gggcatattg tatattgttt
     6541 ttcaattgat tacactaaag aatcaaaatt tgaagtatct caattaggtg ctattcctat
     6601 agaatataag ctaagcagat ctgggttaaa tcctttttta gatattaaaa atacctatat
     6661 actatataaa atattaaaaa gacttaatat agaacttact ctttcttatt tttctaaacc
     6721 agcaatattt ggtacaattg ccgcaaaatt agctaatact cctcaaatat atgcaatgct
     6781 agaaggactg ggatattatt ttactgaaca aaaaaatgga ctaagtctaa aaacaaaaat
     6841 agttaaatat attcaaatat tattatataa aatagcattt cctttatcaa atgggttgat
     6901 tctactaaat aatgatgaca agaaggattt aattgaacgc tataaaatta aaacggaggt
     6961 atttatttgg ggggggattg gattagacct taatcaatat aagaaatcag atataattat
     7021 taatgataaa atttcctttt tatttatagg taggctatta aaagaaaaag gaatatttga
     7081 gtatattcat gcggccgaaa aaataaaaaa atcatattct aatattgaat ttatagtatt
     7141 aggtgatata gacatggata acccaggaag ccttacaaaa aaggacttgg acaatttaat
     7201 agaaaaaaat attataacct atcctggaca tgtggataat gtcatagagt ggttaaataa
     7261 aagtcatgtt ttcgtactac cgtcatatag agaaggatta cctagaagta cacaagaagc
     7321 aatggcaata ggtcgagcca tcattactac tgacgttcca gggtgtcgag acactgtttc
     7381 taataaaaaa aatggattgc ttgttaaacc atggtcatct gatgaacttg catctaaaat
     7441 ggaatatcta attaagaata aatctgaaat aataaaaatg ggtaataata gctatagtta
     7501 tgctcaaaaa aatttcgacc aaaagataac aaataaaaaa attttaaatt atatgaaaat
     7561 cacaaacgaa aaataaaatt aattgatgat atacatatac acctgataga tttaactata
     7621 attaataata attttattta atcttataca tctattcttt agaatttata tacacaattc
     7681 atagcagaga aacacatgaa aatcaccatt tcaggaaccg gctatgtcgg tctatctaac
     7741 gggatactcc tttcccagca caacaaagtt gttgcattag atatcaataa agaaaaagtc
     7801 gaactattaa accaaaaaat ctctcctatc tctgataaag aaattgaaga atttttagcc
     7861 actaaaaagc tcgattttac ggcaacttta gataaagaat tcgcttataa agatgctgaa
     7921 tttgttatca ttgccactcc taccgattat gatcctaaaa ctaactactt taacacctct
     7981 tctgtagagt cggttattcg tgatgtttta gaatataatc caaacactac tatgattata
     8041 aaatcaacca ttccagttgg ttttactaaa cagatgagag aaaaatatca taccgataat
     8101 attatcttct ctcctgagtt cttacgtgag ggtcgcgccc tttacgataa cctttatcca
     8161 tctcgtattg tgattggtga gcaatctgca agagcagaga aatttgctga gcttttacaa
     8221 aaaggtgcgt taaaaaaaga tattgatgtt ctttttactg attctacaga agcagaagca
     8281 atcaaactat ttgctaatac ttatctcgca ctgcgtgttg cttattttaa tgaacttgat
     8341 agctacgccc aagcttacgg cttaaacgca agacaaatta ttgaaggtgt ctgtttagat
     8401 ccgcgtattg gtaatcacta taacaacccc tcatttggtt atggcggtta ctgcttacca
     8461 aaagatacta aacaactgtt agccaactac gaatccgtgc ctaataatat tattggcgcc
     8521 attgtggaag ctaaccgcac ccgtaaagac tttattgctg attctattat cgctaaatca
     8581 ccgaagattg ttggcgttta tcgtttaatt atgaaatcag gctctgataa tttccgctca
     8641 tcttcaattc aaggcattat gaagcgtatt aaagcaaaag gcattgaagt ggtaatttat
     8701 gagccagaaa tgaaagaaga gactttcttt aactcaaaac tcattcgtga tttagatgcg
     8761 tttaaagcca tgtctgatgt cattattact aatagaatgg cttctgcact aaacgatgtt
     8821 gaagaaaaag tttatactcg tgatctcttt ggtaacgatt aaaactaatt tatatgctca
     8881 atatcgtctt atttgaaccc gaaattccac cgaatacggg taatattatc cgcctgtgtg
     8941 ccaacacagg ctttcatctt catttgatcc aaccattggg ttttacttgg gatgataaac
     9001 ggctacgccg tgcaggactt gattatcgtg agtttgctga tatcaaacaa caccacgatt
     9061 attatgcttt tttagaaagt gaaggattaa gccctgataa tgcacaatca ccgacaggtg
     9121 cgcgattgtt tgcattaacc actaaaggta cccccgccca tagtaatgta agttatcaag
     9181 atggggatta tctgctattt ggccctgaga cacgaggttt accaccttat gtgctggata
     9241 atatgccaac acaacaaaaa atccgtgtac caatgttagc ggatagtcgc agtatgaatc
     9301 tttcgaatac agttgccgtg gttatttttg aagcatggcg ccaattaggc tatccgggtg
     9361 cgttattaag agattagttt tactaattat tgcttatatt atttcaaaaa cgctttgcat
     9421 tcttgataaa gcgtttttat ttatcatcat tttgcggttt accgcttaaa attaggttat
     9481 aacatgcata atgttacatt atcttttaaa tattctcatc atataagcca gtatatatat
     9541 ggttttttac ttttaagtaa aaacaatata ataaaactat ctaatataga taaagataca
     9601 aatatcactg gagcgcagca tatattaaga gcaaatattg atggtaaaaa aataatatat
     9661 gatgcaaatg atggcgatca tatagataga ggtttttttt ctattcctga ttatgaatgg
     9721 tgtgacttat attttaaaag aagttattcc aatcaactag cggaacaatt tcctaaatgt
     9781 agacctcttg ggtttaatta tgaaataaag cctttttatg gaatgataga tcgttttttt
     9841 ggtgatattc gtaggttaat gggtaaagaa attgtaaagc atactgattt agaaatcata
     9901 cctaacgtat caaataatcc taaaatatta tttctaacta ggttgtggga gcctaaccca
     9961 cttaaagaca atgcgtcaaa aattgagtta gaatactaca atgaaacaat acaacttaat
    10021 aaagtcagaa tggatgcatt gaatatgatc catgcccaat tcaatgaaag atctactatt
    10081 ggtattaatg atattccata ttcaagaaga atggcgccag attttatatt accgaaagaa
    10141 aaaacacatc gaagaaattt tattaatatg atgaaagaac atgaaatatg tattacatca
    10201 actgggttac ataaatcaac aggatggcgc tttggagaat ttgtagctgc atctcgagct
    10261 attatttctg aaccattaaa ttacgtagtt cctggtaatt ttgataatta ccttcctttc
    10321 aataatattg aagaattata tctggcaatt gaaaagctgg ttaatgataa agagttaaga
    10381 tatgacatga tgaaaagaaa caatatttat tataataatt atttaaaacc agataggtta
    10441 atattaaaca caataacttc attataatta actattttta atatacaatt ggctgataaa
    10501 atttatcata tcttatttag gactttatac ccgcctgata tcggcaatca ctttcgttat
    10561 ataccgacac ctttccccca catcctttaa tactaatgca tgggcgccaa taatcgcatt
    10621 atcaccaata ttaatctcac caccaataat ggatgaatta cagccaatat aaacatggtt
    10681 tcctacttgg atattggctt ttccctcttt ctcttttacg ccaatagtca ccccttgcat
    10741 aatagtgaca tattccccca gttggcagta attagtcagt acaatgccaa tcgggtgtgg
    10801 taaagaaagg cctttaccaa tacgagtttt taacgagata tcattacagt atttacggtt
    10861 attgctagca ccgatccgaa tacctaattt atgtaaaaag cgatggggag agaaataaaa
    10921 atattgtgct acccgccacc aaaataggta acgggaacgc tgacagaaaa atactcgatg
    10981 aaatagcctt aaccaactaa agggtttttt atcaccaata tattcagcct gtaagcactc
    11041 ttttagttca gcaagagtaa ccttagcttg agcatgcata gttaaatacc atcaccacac
    11101 tcaaagccgg gtacaacacc attaaagtgt tgatccatat cgaatgacgg tttttcacta
    11161 tcaggacgac cgacaatacg ggcaggcacc cccgccaccg tagtatgagc cggtacttca
    11221 tgtaatacta ccgagccagc cccaattttg gcaccacgac caatctcgat attaccaaga
    11281 attttagctc ctgcgccaat cattacccct tcacgcactt tcggatgacg atcgcctcct
    11341 gttttacctg ttccccctaa ggtcacagat tgtaaaatgg agacatcatt ttcaaccacc
    11401 gccgtttcac caattacaat acctgtcgca tggtcgagca taataccgtg tccaatacgt
    11461 gccgctgggt gaatatccac cccaaaggtg actgatattt ggttttgtag ataaatagct
    11521 agcgcttggc gattttgttg ccataaccaa tgggcaatac ggtaggcttg tagtgcatga
    11581 aaccctttta aatagagcaa tggggtagaa tatttatcca ccgcgggatc acgtaggcgt
    11641 accgcagaga gatcacaagc ggccgactca atcattgagc tatcgtgact ataagcctct
    11701 tcaacgattt cgcgtactgc aatggctggc atagtctgag ttgccagctt attggccagc
    11761 atatagctta atgcactacc taaattttcg tgtttgagaa gtgtcgcatg aaagaaactg
    11821 gctaacattg gctcacaatc tgccaatgca cgcgcttcgc ttttaatatg atcccagact
    11881 cttgttagtt cttctagcga catactcact ctcatttttt acacgaaagc ctaagtaaat
    11941 acttaggctt tgaattttca aacaaatgga tagttaaaac agatacccta agaatattat
    12001 ttaaaataaa ctgactacag gctgctgtct atctcatctt tggttgctcg ccctaataaa
    12061 gccaatgcag cctcttttac atctttatgt tgatataaaa tttggtaaat ctgttcggtg
    12121 attggcattt ctacacccac ttgttcggct aatgcacgaa cttctttggt gttacgataa
    12181 ccttcgacta cctggccaat tttctcttgg gctgtatcaa catctaaacc ttgacctagc
    12241 atcataccaa agcgacgatt acgtgattga ttgtcagtac aggttaaaac taaatcaccc
    12301 aaaccagcca tgcccataaa agttgccgca tctgcaccta aggctttacc taagcggctc
    12361 atttcggcca gaccacgggt aataagtgcg gtacgcgcat tagcgccaaa tcccatacca
    12421 tcagacatac ccgcaccaat agcaatcacg tttttaacag cgccacccag ttgcacaccg
    12481 ataaaatcag ggtttttata gactcggaag cttttaccac aatgaaatag ctgttgtaac
    12541 tgttctaaaa agagattgtc tgtcgaggcc actgcaatcg cggtgggcaa acccgcagca
    12601 agctctttag caaaagtagg gccagataac actgcgagcg ggatttcatt acctaatact
    12661 tcacgggcaa catcttgtaa taagcgacca gtatgtgctt caagaccttt tgtcgcccaa
    12721 acaacacgtg catcctgacg taaaaagggt ttgatttgtt gcagtacttc accaaacaca
    12781 tggcttggga tcaccacaag aatattacgg ctcgcttcaa tcgctttttg caaagaagct
    12841 tccatatata aactatcagg aaaggaaaca tcgggcagaa aggcttgatt acagcgcgct
    12901 tgttctaatg ccgcaacgtg cttgggatca tgcccccaca gcacaacatc gtgcccatta
    12961 cgcgctaagg taatcgctaa agcggtgccg tatgaaccgg caccgataac tgtcatagaa
    13021 gcgttcatta agcttcttca actcctgagt ttgctgcatc ctgttgctgt tgttgctgta
    13081 agtagttcat aaacaacgca tcaaagttaa caggtgctaa gtttaattgt gggaaagtac
    13141 cacgacctac taagttggtg atacattcac gtgcataagg gaatagtacg ttcgggcagt
    13201 atgcacctaa gcaatgtgct aattgtgtac cttcgatacc ttcaacagta aagatacctg
    13261 cttgttgtac ttcacccagg aaagcggagt gaaagtgaat accgtcaagg tgttatgctt
    13321 gttgtacttc acacaggaaa gcggtgggcc cctggggaaa aagtgaatac cgtcaaggtg
    13381 ttatgcttgt tgtacttcac
//
