LOCUS       KY710687               16013 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 12/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710687
VERSION     KY710687.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 12/57"
                     /serotype="O5"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..16013
                     /note="O antigen gene cluster"
     gene            55..586
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             669..1814
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /gene="glf"
                     /product="glf"
                     /protein_id="AXY99348.1"
                     /translation="MKFLVVGCGLTGAVISRHLADAGHCITIIDQRSHIAGNCHTERD
                     RNTGIMEHVYGPHIFHTSDEVVWEYINKHGEMIPYVNRVKTTYQNQVYSLPINLHTIN
                     QFYQKALNPEQAKAFLSTITDKSITDPKNFEEQALKFIGKDLYEAFFKGYTVKQWGCD
                     PKNLPASILKRLPVRFTYDDNYYSHKYQGMPKHGYTDIVRSILDHKNIQVITNKKYDH
                     SESKEYDHIIWTGKIDEWFGYCEGRLNYRTLDFIKHETIGDYQGTAVMNYGDIAVPYT
                     RIHEHKHFSPWESHDKTIYIEEHSRVCEENDIPYYPIHLEEENKILNKYLELAQKEKY
                     ITFAGRLGTYRYMDMDVSIKEALMLAENLVSDLINQNELKSFYHKHQ"
     CDS             1829..2587
                     /codon_start=1
                     /transl_table=11
                     /product="wemA"
                     /gene="wemA"
                     /protein_id="AXY99349.1"
                     /translation="MSKFLAVIRCGDNSLHKNWVNDNAQFDVILSYFGEDIPYSLDNI
                     KYVHHFKGSKWEGLYDLFHNHPELWADYDYIWLPDDDLDSTVENINLFFELMQKYRFD
                     LCQPALTNNSYYSYKDLLQEPDLIYRETNFVEVMAPCFSKKIISKAYQTFNENKSGWG
                     LDFYWPILFKEENIKVGVIDSTPIHHTRPVGIAGHGSSDKTLSPLNELHQLLDKYSLE
                     IPTFVATSLLTQKGKMIQKGSFSYSLREFMKKRK"
     CDS             3280..3849
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99350.1"
                     /translation="MTGRNFFKYFGKFISLISFLLSVFPKKFNLFLFNLFSGCPTYCG
                     TLIRYILLKSIIKDLGNNVYIGRWCIIKNINNLTIGNNVSIHDMCYIDALGGIKIGNN
                     VSIAHQCSLISFEHNYNDEQTIPIKYQRIIKKNIILHDNIWIGCGSRILAGTIIPQKT
                     VIAANTVTKGELKQGVNAGSPARKIKDIL"
     CDS             3846..5021
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99351.1"
                     /translation="MKLLFLHDHYIYVDKNNNAFSPGKLSSESFSRYLNHFDNVTVLT
                     RSKLIDEDLSNSLNFNQINTDNINYIPFDNQSNIKNRFFKRQLYKRKLKKIINQYDAI
                     IVRLPSEIGFLAAEICISLHKPYVSEVVACPVDAMDGFDNLKSYLYKPIIKRNMEKYV
                     YNSIGSLYVTRFFLQRKYPTKGFQCSASNVEIEKVSFIKRDISNKLKINITLIGNLDS
                     PHKGYNILYKALSLLDDKLIDKSLIIYLVGPGNKYKIIPSYKNIMIKYTGSLNKKEVF
                     KLLYNTDIYIQPSNQEGLPRATIEAMSLSIPCITSNAGGLPELTHEKCIHNTNDYKAL
                     AEKILFLINNHDFYKELSSINANKSENYLNEKLSLIRSDFFNKYKSLLISKIENEKK"
     CDS             5008..6150
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99352.1"
                     /translation="MKKNNSLVKNLSLISFFLIYLSLITDKLKYNLEANILIVITFCI
                     SLIILLYNNLKITNKDIVLTYIIYFSVFFGIISTILANDIEGLISIIKFFCFIFIGST
                     ISISILRNIEVISILNTYYLIVFSLIIIFLYGDNLGRNFKGPFLNQNSLGMILSLGVY
                     FSLLSIFINKNKIKITISFFALFLSVPILLYTNSRTAILAPFLAIGVVFLYSLFKINK
                     LHIKKILISFFFISLIIISLFLLQDILNETILEKFNRKSGDLTSGRLEFIKATIPYLN
                     YIYSTKVPEYIIIDNTLVAYAANFGIISLSFFVLIYFLYPITFIYIRKKLTYTQSLFI
                     SSFYIYYFFYSLLESIRISPNTLIFLTIYFFLCYKLKNRNNEAIKR"
     CDS             6131..7315
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99353.1"
                     /translation="MKLLKDNIYNLIGNITYALSQSLVIIILNKLGNPIDVGAFTYGM
                     AVIIPFFILAQGGLRSIISIDAKNNYNFNIYFYLRLICCVIALLLVALINISNKYFTI
                     IILLATFKFMDSIFDIYYGQYQRDSNFKSIYKSRVIRSISNILSFTVTYYFTKNLIFS
                     LIIYTLSSLIIYLIYDFRVYKINIKNLKHSILFKVFIISLPLAISSFLISLNTNIPRI
                     LLENKVGIITLGAFGSFIYLLNICQIVVQSICQVFSPRLAKYYLDSNYSAFKRNSFLL
                     IFLILLISIIFVLFAIFLQDIIFYLLFNSSFYKYKNIYEYIIYISPIIFLGVALGNIC
                     TAQRKIKIQPYIFSVITIINLTSAYFTISLYGIYGAIFSLLLVALLTCVFLTFYIKFY
                     KR"
     CDS             7320..8423
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99354.1"
                     /translation="MKVLHILGQLDTGGIECWLNDLIEKSNEMDSKIEYTIIVDKVHI
                     GNLEQNFLNLNVKVVHITPNKKSKINYLKDIYNIIKKENFDVIHSHVSFTSGIINAIA
                     YVNKVKIRVTHIHSNRLLEIKRSSFLKKIKIFSLLLLIELFSTNKIAVSNNAKRTFLK
                     KKNSLIIPCGKNFSKIWDKTEISCNLELWKKNCILLISIGRLEKVKNHIFLLELMKHL
                     SDDFKLLIIGDGSEKIHLENFIEKNNLEDRVKLFGISDNCLNIMVNLGNVFLFPSLHE
                     GLGMSAIEAQACGLPVICSTNVPKEIKLTENVFFLPIEKNDKKLWIDKILDLKDKKQG
                     IKEKIITGEYSIENNFNKIHEIYLRNSYPKESK"
     CDS             8420..9532
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99355.1"
                     /translation="MKKIAITSNTSWYLYNFRKNTIISLIEAGFKVYTVSPIDSYSNK
                     LKELGAEHINIKIDSASKNPLIDLYTLISFYKIFKQLRCNAVLNFTPKNNIYGTLAAK
                     LNKIKVINNIAGLGTLFVNDTISCKLARNLYRYSQKYADKIFFQNNDDLSLFLEKKYV
                     DKVQVDRLPGSGVDLSRFTLSLSEYKHKFRFLLVARMLYEKGITFYVEAARILKERYG
                     DKVEFCLLGFVGVNNPSAITHKQMNNWVAEGIINYLGTSDTVENELAQADCIVLPSFY
                     REGVPKTLLEAGAMGKPIITTDNVGCRETVTHGFNGYICQPKSVSSLVDAMDRFINLP
                     YEKKLKMGKNSRQKIETEFDERIVIKKYLDALKEIM"
     CDS             9547..10713
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99356.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             10739..11749
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99357.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEIEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             11753..12256
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99358.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTKQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12515..13372)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt5"
                     /product="glycosyltransferase"
                     /protein_id="AXY99359.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKK"
     CDS             complement(13382..13939)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99360.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGERCRYINKMNFEINKYC"
     CDS             complement(13942..14775)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99361.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14875..15888)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99362.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
BASE COUNT     5585 a   2561 c   2491 g   5376 t
ORIGIN
        1 ggtgaaagta aagagctaga gcgtattgaa acagaaacgc agcggctaga tggcatgatc
       61 aatgacttat tagtgctttc tcgcaaccag tataaaaatg agttattacg cgaaaccgta
      121 aaagcgaatg agctttggaa cgatatttta gataatgcga aatttgaggc tgaacagagc
      181 aataaaactc taacagtaac cgccccgcca ggcccatgga caatttattg caatccttat
      241 tcattagcga gtgcctttga aaatatcgtt cgcaatgcct tgcgttattc acattctcgt
      301 attgaagtgg cttttaccga aacaaaatca gggtatcact attattgttg atgatgatgg
      361 cccaggggtt agcccagaag atcgcgagca tattttccga cctttttatc gtacggacga
      421 agcacgtgat agagaatccg gcggcacagg gctgggactg gcaattgttg aaaccgcgat
      481 tagtcaacat cgaggccatg taaaagccga cgatagccca ttagggggat taagagtgga
      541 gatttggttg ccgagaggga gtattagctc aagcaataaa ttataattat catttttaac
      601 tattgataat tactgtatat tttaccctat taatttatac aaaatgtact aagttcttag
      661 ggtaaaatat gaaattttta gtcgtaggct gtgggttaac aggggctgta attagccgcc
      721 atctagctga tgctggtcat tgcatcacga taatagacca aagatcacat attgctggta
      781 attgccatac agaacgagat agaaataccg gtattatgga acatgtttat ggtccacata
      841 tcttccacac tagcgatgaa gtggtatggg aatatatcaa taaacatggt gaaatgatcc
      901 cttatgtaaa tagagtaaaa acaacttatc aaaatcaagt atattcttta cctattaacc
      961 tacatacaat aaatcaattc taccaaaaag cgctaaatcc agaacaagca aaagcttttt
     1021 taagtactat cacagataaa tctattactg atcctaaaaa ctttgaggaa caagccctaa
     1081 aatttattgg aaaagattta tatgaagcct tttttaaagg ctatacagta aaacaatggg
     1141 gttgcgaccc taaaaatcta cctgcgagta ttttaaaacg cctccctgta agatttactt
     1201 atgatgacaa ttattattct cataaatacc aaggaatgcc taaacatggc tataccgata
     1261 tagttcgttc tatccttgat cataaaaata tacaagtaat aacaaataaa aagtatgacc
     1321 atagcgaaag caaagaatat gaccatatta tatggactgg aaaaatagat gaatggtttg
     1381 gctattgcga aggtagatta aactatcgaa cgctagattt tataaaacat gaaactatag
     1441 gtgactatca aggtacagct gttatgaatt acggcgatat cgcggtacct tatactcgca
     1501 tacatgaaca taaacacttc tcaccatggg aatctcatga caaaacgatt tatattgaag
     1561 aacatagtcg ggtatgcgaa gagaatgata ttccttacta tcccattcat ttagaagaag
     1621 aaaataaaat attaaacaaa tacttagaat tggctcaaaa agaaaaatat atcacttttg
     1681 caggtaggct aggtacatat agatatatgg atatggatgt atcaattaaa gaagcactta
     1741 tgcttgcaga aaacttagta tcagatttaa taaatcaaaa tgaacttaag tcgttctacc
     1801 acaaacacca ataacaagag aatattctat gtcaaaattt ttagcagtaa ttagatgtgg
     1861 agataattca cttcataaaa actgggttaa tgataatgcc caatttgatg taattcttag
     1921 ttattttggt gaagatatcc cttattcttt agataatata aaatatgtac accactttaa
     1981 aggatcaaaa tgggaaggat tatacgattt attccataac catccagagt tatgggctga
     2041 ttatgattat atctggttgc ctgatgatga cttagacagt accgtagaaa atatcaactt
     2101 attttttgaa ttaatgcaaa agtatcgatt cgatctttgc caaccagcat taacgaataa
     2161 tagttactat tcctataaag acttactaca agaaccggat ttaatatata gagaaactaa
     2221 ttttgtagaa gtaatggcgc cctgcttttc aaagaaaatc atttctaaag catatcaaac
     2281 atttaatgaa aataaatcag ggtggggact agatttttat tggcctattt tatttaaaga
     2341 agaaaatatt aaagttggtg ttatagactc tacaccaata catcatacac gaccagtagg
     2401 gatagcaggc catggaagta gtgataaaac cttatcgcct ttaaatgaac ttcatcaatt
     2461 attagacaag tattcacttg aaatacctac gtttgtagca acttcacttc taacccaaaa
     2521 agggaaaatg atacaaaaag gcagtttcag ctattcttta agagagttta tgaaaaaaag
     2581 aaaataaccc tatgattaaa ttaagaaatt taccataaaa tcacaataaa actactttta
     2641 tatcaaccat aaggactgtt tttatattga ataaatgcag tctaattacc tgatacaaag
     2701 tagatacatt ttgagtaatg tagttttaac gcttacagca atacaataaa aaaattacta
     2761 cattaaaata ttctgccatt tattgaagag tatgtggcat attcttttca ttcccatctt
     2821 tatagatatt tcagaatgat cttgctaaat cagtcgatat tccgatactt atatatacct
     2881 aatcaatgtt agaataccca tcaattactt gacccgctgg tttgaagagt ctaatctcaa
     2941 agcggtttag catactgtta aagtatgctt atataataaa actcttatat tacagataga
     3001 taagggtttt aaaagagaaa ttttggcgct ctgggggccg taaacccagt gcggtagctt
     3061 tagccgtttt aattattaaa gtagttcttg ttattaaata cagtataatc gggcctaaag
     3121 acaatatttt tcatatttta ggctatgtat aatcaggagc agaaggtaat gaatcagtat
     3181 tatttactat taaatagaat gcaattaacg ttttattaaa aaaatagata atattaataa
     3241 tgaaagacca agaatcgtag ctatgaagaa ataatcatta tgaccggaag aaattttttt
     3301 aaatattttg gaaaatttat atctttaatt tcatttttat tatcagtttt ccctaaaaaa
     3361 tttaatttat ttttatttaa tctatttagt ggatgcccta catactgtgg taccctcata
     3421 agatatatat tattaaaatc aatcattaaa gatttaggta ataatgtcta tattggacga
     3481 tggtgtataa taaaaaacat taacaatctg actattggta ataatgttag tattcatgat
     3541 atgtgttata tagatgctct aggcggtata aaaatcggta ataatgtatc tatagctcac
     3601 caatgtagtc taatatcttt tgaacataac tataatgatg agcaaacaat accgataaaa
     3661 tatcagagaa ttataaaaaa aaatataata ttacatgata atatttggat tggatgtggt
     3721 agccgtattt tagctggtac aattattcct caaaaaacag ttattgcagc taacactgta
     3781 actaaaggtg aattaaaaca aggagtaaat gcaggaagcc cagcaagaaa aattaaggat
     3841 atactatgaa gttattattt ctacatgacc attatatata tgttgataaa aataataatg
     3901 cattttcccc gggtaaactc tccagtgaat ctttttctcg ttatctaaat cattttgata
     3961 atgttacagt tctaactaga agtaaactta ttgatgaaga tctatctaat tctttaaatt
     4021 ttaatcaaat aaatactgat aacattaatt atattccttt tgataatcaa tcaaatatta
     4081 aaaatagatt ttttaagcga caactttaca agagaaaatt aaaaaaaata attaatcaat
     4141 atgatgctat tatagttagg cttcccagtg aaattggctt cttggcagca gaaatatgta
     4201 tttcactaca taagccttat gtatctgagg ttgttgcttg tccagttgat gctatggatg
     4261 gttttgataa tttaaaatct tacctatata agccaattat aaaaagaaac atggaaaaat
     4321 atgtctataa ttctataggt agtctatatg ttactcgttt ttttttacag agaaaatatc
     4381 caactaaagg attccagtgc agtgcatcaa atgtagaaat tgagaaggtt tcttttataa
     4441 aaagagatat aagcaataaa ttgaaaataa acattacatt aataggtaat cttgatagtc
     4501 ctcataaagg ttataatatt ttatataaag cattaagttt attagatgat aaacttatag
     4561 ataaatcact aataatttat ttagttggac ctggtaataa atataaaatt attccttcat
     4621 ataaaaatat tatgattaag tatacaggtt cactgaataa aaaagaggta tttaaactgt
     4681 tatataatac cgatatctat atccaaccca gtaaccaaga aggattgcct agagcaacaa
     4741 tagaagcaat gagtttatct ataccatgta tcaccagcaa tgcgggagga ttaccagaat
     4801 taactcacga aaagtgcata cataatacta atgattataa agctcttgca gaaaaaatac
     4861 tatttttaat aaataatcat gatttttata aagagctttc ttcaattaat gccaataaat
     4921 ctgaaaatta tttaaacgaa aaattatctc ttattagatc tgattttttc aataaatata
     4981 agtcattact aatctcaaaa attgaaaatg aaaaaaaata atagtttagt aaaaaattta
     5041 tcgctcatct ctttctttct tatctatcta tcattgataa cagataaact aaaatataac
     5101 ttagaagcga atatacttat tgttatcaca ttttgtatat ctttaataat attattatat
     5161 aataatctta aaattactaa taaagatata gtattaactt atataattta ctttagtgta
     5221 ttttttggta ttatatctac aatattagct aatgacatag aaggcttaat atctataatt
     5281 aaattttttt gttttatttt tattggtagt acaatttcta tttctatatt aagaaatata
     5341 gaagttattt caatattaaa tacttattat ctaattgttt tttcattgat tatcattttt
     5401 ctttatggag ataacttagg acggaacttt aaaggtccat ttttaaatca aaattctctt
     5461 ggtatgattt tatcattggg tgtttatttt tctttattat caatatttat taataaaaat
     5521 aaaattaaaa taactatttc attttttgct ttatttttat cagttcctat tcttctatat
     5581 acaaatagtc gaacagctat tttagctcct tttttagcca taggagtagt tttcttgtat
     5641 tctcttttta aaatcaataa attgcatata aaaaaaatat taatatcatt cttttttatt
     5701 agtttaataa ttatttcttt atttttatta caagatattc ttaatgaaac aatactagaa
     5761 aaatttaatc gtaaatctgg tgatttgact agtggaagat tagaatttat aaaagcaaca
     5821 ataccttatt tgaattatat atattccact aaagttcctg aatatataat tatagataac
     5881 acattagttg catatgctgc taattttgga attatctctt tgtctttctt tgtattaata
     5941 tattttctat atcctattac ctttatatat ataagaaaaa aattgacata tactcaatcc
     6001 ttatttatat ctagtttcta tatatattat tttttctatt cattactaga aagtattagg
     6061 atatccccta acacattaat atttttaaca atttattttt tcctttgtta taaattaaaa
     6121 aatagaaata atgaagctat taaaagataa tatatacaac cttattggaa atattactta
     6181 tgctttatca cagtcattag taattataat tctaaacaaa ctaggtaatc ctatcgatgt
     6241 aggtgctttt acttatggta tggctgttat aatacccttt tttatattgg ctcaaggagg
     6301 actaagatct attatttcta ttgatgcaaa aaataattat aattttaata tttattttta
     6361 tttaagacta atttgttgtg ttattgcact tttgttagta gctctaatta atataagtaa
     6421 taaatacttt acaataataa ttctgcttgc aacatttaaa tttatggatt ctatttttga
     6481 tatatactat gggcaatatc agagagatag taactttaaa agtatttata aatcaagagt
     6541 aattagatcc attagcaata tattatcatt tacagtaaca tactatttta ctaaaaatct
     6601 tattttttca ttaataattt atacattatc ctcattaatt atttacctaa tatacgattt
     6661 tagagtatac aaaataaata ttaaaaatct gaagcactct attcttttta aagtttttat
     6721 aatatcactt ccattagcaa tatcatcatt tctaatatca ttaaatacta atatacctcg
     6781 tatattatta gaaaataaag ttggaatcat tacattagga gcctttggaa gctttattta
     6841 cttgctgaac atctgtcaaa ttgtagttca atctatatgc caagtgttct ctcctcgtct
     6901 agccaaatac tatttagata gtaattactc tgcatttaaa agaaacagtt ttttactgat
     6961 ttttttaata cttttaatat ctattatttt tgttttattt gcaatttttt tacaagatat
     7021 aatattttat ttattattta attcttcttt ctacaagtat aaaaatatat atgaatatat
     7081 aatttacatt tctcccatta ttttcctagg tgttgcccta gggaacatat gcactgctca
     7141 acgaaaaata aaaattcaac cctatatttt ttcagtcata acaattatta atctaacttc
     7201 agcatatttt actatcagtt tatatggtat ctatggtgct atattttctt tgctattagt
     7261 agcattattg acttgtgtat ttttaacgtt ttacataaaa ttttacaaaa ggtaacgcaa
     7321 tgaaagtatt gcatatttta gggcaattag ataccggtgg aatagaatgc tggttaaatg
     7381 atttaataga aaaatctaat gaaatggatt caaagattga atatactata attgtagata
     7441 aagtacatat aggtaatctt gaacaaaatt ttttaaatct aaatgttaaa gttgtacata
     7501 taacaccaaa taaaaaatca aaaataaatt atttgaaaga tatttataat attataaaaa
     7561 aagaaaattt tgatgtcata cactcacatg taagctttac ttctggaatt attaatgcaa
     7621 tagcttatgt taataaagtt aaaataagag ttactcatat acatagtaat agattgctag
     7681 aaataaaaag atcttctttt ttaaaaaaaa taaaaatatt ttccttacta ttattaatag
     7741 aacttttttc tacaaataaa atagcagtga gtaacaacgc aaaaaggaca tttttgaaga
     7801 aaaaaaatag tcttattatt ccttgtggga aaaatttttc aaaaatatgg gataaaacag
     7861 agatctcttg taacttagaa ttatggaaaa aaaattgtat attattaata agtataggtc
     7921 gattagaaaa agttaaaaat catatttttc tattagaatt aatgaagcat cttagcgatg
     7981 attttaagct attaattata ggagatggtt cagaaaaaat tcatttagaa aactttattg
     8041 aaaagaataa tcttgaagat agagtaaaat tatttggaat tagtgataac tgcttaaata
     8101 taatggtaaa tttaggtaat gtctttcttt tcccttcgtt acatgaagga ttaggaatgt
     8161 cagcaataga agctcaagct tgcggactcc ctgtaatttg ctcaactaat gttcctaagg
     8221 aaataaagct aacagaaaat gtattttttc tacctataga aaaaaatgat aagaaattat
     8281 ggatagataa gatcttagat ttaaaagata aaaagcaagg gataaaagaa aaaattatta
     8341 caggagaata ttctattgaa aataatttta ataaaataca tgaaatctac ttgcgcaact
     8401 cttacccaaa agaatcaaaa tgaaaaaaat tgccattaca tcaaacacca gttggtattt
     8461 atataacttt agaaaaaata ctataatatc tttaatagaa gctggattta aagtttatac
     8521 tgtttcacct attgatagtt actctaacaa actaaaagaa ttaggtgcag aacatataaa
     8581 tatcaaaatt gattcagcca gtaaaaatcc tttgattgac ctttatacat tgatttcatt
     8641 ttataaaata tttaaacaac ttagatgtaa tgctgtactt aattttactc ctaaaaataa
     8701 tatttatgga acattagcag caaaattaaa caaaataaaa gtcatcaata atattgcagg
     8761 tttaggtaca ttatttgtaa atgatactat tagttgcaaa ctagcaagaa acttataccg
     8821 ctatagccaa aaatatgctg ataaaatatt ttttcaaaat aacgatgacc tttcattatt
     8881 tttagaaaaa aaatatgtcg ataaagtaca agttgataga ttgccaggtt caggtgttga
     8941 tttatctcgc tttacattat cattatcaga atataaacat aaatttagat ttctattagt
     9001 ggcaagaatg ctttatgaaa aagggattac tttttatgtt gaagctgcac gtatcttaaa
     9061 agaaagatat ggcgataaag ttgaattttg tcttttagga tttgttggtg taaataatcc
     9121 ttcagcaatc acacataaac aaatgaataa ctgggtagct gagggaatta ttaattattt
     9181 aggtacatct gataccgtag aaaatgaatt agcccaagca gattgtatag tgctaccttc
     9241 tttttaccga gaaggagtac caaaaacatt actagaagct ggagctatgg gaaaacccat
     9301 aatcactact gataatgttg gttgtcgaga aacagttact catggtttta atggatatat
     9361 atgtcaacct aagtctgtat caagcttagt tgatgccatg gatagattta ttaacttacc
     9421 ctatgaaaaa aaattaaaaa tgggtaaaaa tagtaggcag aaaatcgaaa ctgaatttga
     9481 tgaaagaata gtcataaaaa aatatttaga tgcgttaaaa gaaattatgt aactttagga
     9541 taaaaaatga aaatcaccat ctcaggaacc ggctatgtcg gtctatctaa cgggatactc
     9601 ctttcccagc acaacaaagt tgttgcatta gatatcaata aagaaaaagt cgaactatta
     9661 aacaaaaaaa tctctcctat ctctgataaa gaaattgaag agtttttagc tactaaagag
     9721 ctcgacttta cagcaacttt agataaagaa ttcgcttata aagatgctga atttgttatc
     9781 attgccactc ctaccgatta tgatcctaaa actaactact ttaacacctc ttctgtagag
     9841 tcggttattc gtgatgtttt agaatataat ccaaacacta ctatgattat aaaatcaacc
     9901 attccagttg gttttactaa acagatgaga gaaaaatatc ataccgataa tattatcttc
     9961 tctcctgagt tcttacgtga aggtcgcgcc ctttacgata acctctatcc gtctcggatt
    10021 gtgattggtg agcaatctgc aagagcagag aaatttgctg agcttttaca acaaggggca
    10081 ttaaaaaaag atattgatgt tctttttact gactctacag aagcagaagc aatcaaacta
    10141 tttgctaata cttatctcgc actgcgtgtt gcttatttta atgaactcga tagctacgcc
    10201 caagcttaca atttaaatgc aagacaaatt attgaaggtg tttgtttaga tccgcgtatt
    10261 ggtaatcact ataacaaccc ctcatttggt tatggtggct actgcttacc aaaagatact
    10321 aaacaactat tagccaacta cgaatcagtg cctaataata ttattggcgc cattgtggaa
    10381 gctaaccgca ctcgtaaaga ctttattgct gattctatta tcgctaaatc accgaagatt
    10441 gttggtgttt atcgtttaat tatgaaatca ggctctgata atttccgctc ctcttcaatt
    10501 caaggcatta tgaagcgtat taaagcaaaa ggtattgaag tggtaattta tgagccagaa
    10561 atgaaagaag agactttctt taactcaaaa ctaattcata atttagatga gtttaaagca
    10621 atgtctgatg tcattattac caatagaatg gcttcagcac taaacgatgt tgaagaaaaa
    10681 gtttatactc gagacctctt tggtaacgat taattaatag aaacggatta attacgtaat
    10741 gaaatattta gtcacaggtg ctgctggatt tattggtttt catctaataa aaaaactgat
    10801 ccagcagggt gaaaccgttg ttggtattga taaccttaat gattattatg atgttgcttt
    10861 aaaagaagct agacttaatc ttttaaatca actggataat ttctcttttt cttttattga
    10921 tttagctgat agagaaaaaa ttgctcaatt atttgagatt gagaaatttg atagggttat
    10981 tcatttagct gcacaagctg gtgttagata ttcactaatt aatccgtttt cttacgctga
    11041 tagtaattta actggttttc taactatttt agaaggttgc cgacacaata acgttaagca
    11101 tcttgtttat gcatcatcaa gttcagtcta tggcttaaat gatgaattgc cattttctcc
    11161 tcatgatcag gctaatcatc ctgtttcttt atatgcagcc actaaaaaag ctaatgaatt
    11221 aatggcgcat agctattctc atttatatgg tattccgact actggattac gttttttcac
    11281 agtctatggc ccttggggac gccccgatat ggcattattt aaatttacta aagccattat
    11341 taacaatcag cctatagata tctataacca tggtgaaatg aaacgtgatt tcacttatgt
    11401 tgaagatatt gtagaaggtg ttacacgtat tgctgatgtg atccctacag cccagcaaga
    11461 ttggaaagta agtacaggta ctccagcaga tagttctgca ccctataagg tttataatat
    11521 tggtaatgga tctccggtaa atttaatgga ttacattagt gcattagaaa tacatttagg
    11581 taaaaaagca gataaaaata tgttaccaat gcaacccggt gatgtgtaca ccacatgggc
    11641 agatactgag gatttattta aagcaactgg ctataaacct caaacaagcg ttgatgaagg
    11701 tgtaaaacag ttcgttgatt ggtataaaaa ctattatcaa gtaaaataaa taatgctcaa
    11761 tatcgtctta tttgaacccg aaattccacc gaatacgggt aatattatcc gcctgtgtgc
    11821 caacacaggc tttcatcttc atttgatcca accattgggt tttacttggg atgataaacg
    11881 gctacgccgt gcagggcttg attatcgtga gtttgctgat atcaaacagc atcacgatta
    11941 ttatgctttt ttagaaagtg aaggattaag ccctgataat gcacaatcac cgacaggtgc
    12001 gcggttgttt gcattaacca ctaaaggtac tcccgcccat agtaatgtaa gttatcaaga
    12061 tggggattat ctgctatttg gccctgagac acgaggttta ccaccttatg tgttggataa
    12121 tatgccaaca aaacaaaaaa tccgtgtacc aatgttagcg gatagtcgca gtatgaatct
    12181 ttctaatacg gttgccgtgg ttgtttttga ggcatggcgt cagttaggct atccgggggc
    12241 attattaaga gattaattta tctttttaat ttataaccat aaaaaatgac ctctagactt
    12301 cattgtctcg aggtcatttt tatgtatatg ctttgtgcta gttttagctt ttcttatttt
    12361 atatcttctt tttatctttt ttatctttcg gttttatttc ttagcttttt tagttatttt
    12421 tcttatcaag aaataccatc ttgcacttac ttttattaaa aatgatgaaa aatcagttac
    12481 taatatcttt ctttctaata atcttttctt aaattcattt tttttgtatt aactcttttc
    12541 gatactcttt agcattaaat tgttggttaa tcttttcaag taacctttta cagcgtacta
    12601 cttgctctac tgttttttta tcattatcca ataataatga ataatcctct ttattcacgt
    12661 taataccaaa atacattaat aattttcgtc ctactctttc cgcggtatct ttttgtatcg
    12721 ctttatattg cgaacgttcg acatttggat gagtacgata ataaagaagt ggtacaggaa
    12781 gattatggag cttaaatttt ccagcaataa ttaaattcac ccataaacca taatcttctg
    12841 catagaggtc tgctttatca tatccaccaa cagataatat atcttgctta cgatagataa
    12901 tggttggatg cgctaaggag caatcataaa gactagaaat cacaatatca ctatgtgttt
    12961 ctggataagt attaattcta tttggctctt cttggtagat aaaagcacca ccacaaacac
    13021 tcacttcagg atgttgtaac ataaattgat attgcgcttc tagtctggtt gaaacggcaa
    13081 tatcatctga gtccattcta gcgactaaat cacatgaagc taattgtaat cctttgttta
    13141 aagaagcgat caatccgata ttttgttcat taataatcag ttttattctg ggatcttttt
    13201 tggcgttttc ttttaagata gataatgagt tatccgttga gccatcatta atgataagaa
    13261 actcaaattc tttaaatgtt tgatttaata tagaatcaat agactcctga aggtatttct
    13321 catcattgta taccgacatt aatacggtaa ttttgggcat attgtttttc attatctatt
    13381 ttcagcaata tttattaatt tcaaaattca tcttattgat ataccgacac ctttccccca
    13441 catcctttaa tactaatgca tgggcgccaa taatcgcatt atcaccaata ttaatctctc
    13501 caccaataat ggatgaatta cagccaatat aaacatgatc tcctacttgg atattggctt
    13561 ttcccccttt ctcttttacg ccaatagtca ccccttgcat aatagtgaca tattccccca
    13621 gttggcagta attagtcagt acaatgccaa tcgggtgtgg taaagaaagg cctttaccaa
    13681 tacgagtttt taacgagata tcattacagt atttacggtt attgctagca ccgatccgaa
    13741 tacctaattt atgtaaaaag cgatggggag agaaataaaa atactgtgct acccgccacc
    13801 aaaataggta acgggaacgc tgacagaaaa atactcgatg aaatagtctt aaccaactaa
    13861 agggtttttt atcaccaata tattcagcct gtaagcaatc ttttagttca gcaagggtaa
    13921 ccttagcttg agcatgcata gttaaatacc atcaccacac tcaaagccgg gtacaacacc
    13981 attaaagtgt tgatccatat cgaatgacgg tttttcacta tcaggacgac cgacaatgcg
    14041 ggcaggcacc cccgccaccg tagtatgagc cggtacttca tgtaatacta ccgagccagc
    14101 accaatttta gcaccacgac caatctcgat attaccaaga attttagctc ctgcgccaat
    14161 cattacccct tcacgcactt tcgggtgacg atcacctcct gttttacctg ttccccctaa
    14221 ggtcacagat tgtaaaatgg agacatcatt ttcaaccacc gccgtttcac caatcacaat
    14281 acctgtcgca tggtcaagca taataccgtg tccaatacgt gccgctgggt ggatatccac
    14341 cccaaaggtg actgatattt ggttttgtag ataaatagct agcgcttggc gattttgttg
    14401 ccatagccaa tgggcaatac ggtaggcttg tagtgcatga aaccctttta aatagagcag
    14461 tggggtagaa tatttatcca ccgcgggatc acgtaggcgt accgcggaga gatcacaagc
    14521 agccgactca atcattgaac tatcgtgacg ataagcctct tcaacgattt cgcgtactgc
    14581 aatggctggc atagtctgag ttgccagctt attggccagc atatagctta atgcactacc
    14641 taaattttcg tgtttgagaa gtgtcgcatg aaagaaactg gctaacattg gctcacaatc
    14701 tgccaatgca cgcgcttcgc ttttaatatg atcccagact cttgttagtt cttctagcga
    14761 catactcact ctcatttttt acacgaaagc ctaagtaaat acttaggctt tgaattttca
    14821 aacaaatgga tagttaaaac agatacccta agaatattat ttaaaataaa ctgactacag
    14881 gctgctgtct atctcatctt tggttgctcg ccctaataaa gccaatgcag cttcttttac
    14941 atctttatgt tgatataaaa tttggtaaat ctgttcggtg attggcattt ctacacccac
    15001 ttgttcggct aatgcgcgaa cttctttggt gttacgataa ccttcgacta cctggccaat
    15061 tttctcttgg gctgtatcaa catctaaacc ttgacctagc atcataccga agcgacgatt
    15121 acgtgattgg ttgtcagtac aggttaaaac taaatcaccc aaaccagcca tgcccataaa
    15181 cgttgccgca tctgcaccta aggctttacc taagcggctc atttcggcca gaccacgggt
    15241 aataagtgca gtacgcgcat tagcgccaaa tcccatacca tcagacatac ccgcaccaat
    15301 agcaatcacg tttttaacag cgccccccag ttgcacaccg ataaaatcag ggtttttata
    15361 gactcggaag cttttaccac aatgaaatag ctgttgtaac tgctctaaaa agagattgtc
    15421 tgtcgaggcc actgcaatcg cggtgggcaa acccgccgca agctctttag caaaagtagg
    15481 gccagataat actgcgagcg ggatttcatt acctaatact tcacgggcaa catcttgtaa
    15541 taagcgacca gtatgtgctt caagaccttt tgtcgcccaa acaacacgtg catcctgacg
    15601 taaaaagggt ttgatttgtt gcagtacttc accaaacaca tggcttggga tcaccacaag
    15661 aatattacgg ctcgcttcaa tcgctttttg caaagaagct tccatatata aactatcagg
    15721 aaaggaaaca tcgggcagaa aggcttgatt acagcgcgct tgttctaatg ccgcaacgtg
    15781 cttgggatca tgcccccaca gcacaacatc gtgcccatta cgcgctaagg taatcgctaa
    15841 agcggtgccg tatgaaccgg caccgataac tgtcatagaa gcgttcatta agcttcttca
    15901 actcctgagt ttgctgcatc ctgttgctgt tgttgctgta agtagttcat aaacaacgca
    15961 tcaaagttaa caggtgctaa gtttaattgt gggaaagtac cacgacctac taa
//
