LOCUS       KY710718               14441 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain TG332 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710718
VERSION     KY710718.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="TG332"
                     /serotype="O50"
                     /db_xref="taxon:584"
                     /collected_by="University of Toronto, Canada"
     misc_feature    1..14441
                     /note="O antigen gene cluster"
     gene            22..1359
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1527..2720
                     /note="glycerol-3-phosphate cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wffW"
                     /product="wffW"
                     /protein_id="AXY99810.1"
                     /translation="MTRGFEESNLRAVLRTDKVYLYNKTLILQIDKRLARNFWRSGGR
                     KPSAVALFKPSNIKINSSEKMNEFIPPSISIVIPVFNVEKYIGECLDSLLLQTFDKFE
                     VICIIDGSTDNSENIIKSGKYQSLNLKIINIPNSGQSIARNIGIHNATGEFILFLDSD
                     DKIRNDTLKKCIEELEENELDGVFFEGQAFSNTLKKNKMKKYNYNRPIINRIIDGKEL
                     YNISIESHLFTVQPCCYIVKKNIIKSINFIPSVYHEDNAYTIELLFSGKLKKIKIINE
                     KFFLRRLRLNSTMTSIKNIKHTKGYLASIKIFSKIIKESNDKRTKKNLSLYLEKLFLG
                     AIISNSSCTTTDKITNLKDELLKIISDIGIKNVTLKTKTLPYFIPYFSLRKNLIKKIR
                     SFKCQ"
     CDS             2711..4135
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /gene="wzx"
                     /protein_id="AXY99811.1"
                     /translation="MSVINNAKWVTVSQLFRIITQIISITVLTRFIEPSEFGILALAT
                     IITNFALVIKDLGLSSAIIQKKELTNALKNTVFWLNILFGFSLYILIIILSKPISNYY
                     EQNKLINVLWLLGTIFPISSISALHQALLERDSRFKIIAKIEIISSLFGVVIALILAI
                     NNYGVYSIVYQLILTQLISSILILINKKWVPSFTFNKKQIKSILEFSSNITIFNIINY
                     FSRNADNIIVGSTFSTTILGVYNLAYRIMLFPLQSVTFIANRSLYPIMSRKQENNKEL
                     KKLYLKAIMVVMLITTPMMMGLAYLRAEFVDIFFSENWEYLSELLLWLSLTGILQSAV
                     STTGAVFMAKNRTRILLLLGIFGTTLNLIAFFIGSQFSIIFFAQCYFIANLINLLPPL
                     YCAVKLLGGGINDILKILFSTLLNSILMIIIIYIFNYLFYIENVYFRFSINIFIGFLS
                     YIIILFILNNEAKNIIMSTLKIKR"
     CDS             4139..4711
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="galactoside O-acetyltransferase"
                     /protein_id="AXY99812.1"
                     /translation="MIKNIIFFILNFFSQIIAYFYILKWKLGFNYKDQAISISVIKGK
                     LGYKVRSNFYQRTLNKCGNKLSVHYGAYIAYPQTVIGNRCSIEEYSVISLAEIGNDVI
                     IASNVVLLSGSKHHEIDDLSLKFHDSNIKMTGITLGDNIWIGVNSVIMANIASGTIIG
                     AGSVLTKSIVIENCIYAGIPAKYVRNRGVL"
     CDS             4708..5964
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99813.1"
                     /translation="MNISKFNIFLLITIFFLILFSPSILFFVSGLTLITIACSKPFNK
                     KEYLFFCNLIIFLFGLILYVLSLPNFILFSDDFSSYYNNFLYFLDSNYKNGFNEYNYE
                     FLFPSLNLVLSKFIYGKFPYLMKLGHIIINLLLLFITIIYVSKKEKIKINDFLLFTCC
                     IMIFYKLPQQMHLMRQGYACYFIIMALFSNKICSKLIFIIIGSFFHLSTPIIYLIINA
                     LSKINNKKKLIAYTLLFASTFLLFYYLSKQKIFFHFPLLNKLNYIINYISSDSHVKKS
                     VLETIKSLFYFYPLLILLIIYNIKKIHIKEGYFIIISTLFLISFSFIPGFGIRVYMPI
                     LSIFLGYYYYKFIKHLNNKFKALLLFCIFIFININWLLFSEIYYYRYPIFSSEPLYYL
                     NSLIEEQEKICRECLPQQVEIKNKYR"
     CDS             5968..6888
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99814.1"
                     /translation="MPNISVVIPYYNDSKHIEQCITSVISQTLTPLEIIIIDDCSIDT
                     DGLRLILNKFNFFNIKLFRNNVNMNGAYSRNRGIHEAKGDFIALLDADDFWSMDHLEK
                     NYNFIFKEKADFVYSQCNRNKKNKLIKRKVNDIHHIVGNIENIILYSPPQTNSFFFSS
                     KISPLICFDEKLKRHQDYQFFLDIIRNKNIKVSYLDQYTTYYRYANTNKENIDYSSIF
                     NFWNKNKEKMDKSLLKKKLWNIIAGGLRNRKINPETILFNKDLDNIFYNDKFYNFTKK
                     IKNKMINRIIINIYFYIVIDRFKKLLHFIG"
     CDS             6894..8018
                     /note="orf07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf07"
                     /product="hypothetical protein"
                     /protein_id="AXY99815.1"
                     /translation="MKKRKIHIYSDINYHSYQHINQMLYRQLSTSNKFYTIFLQRFIM
                     NSVRWKYVLKFFSATIDKIKNPHNKNKNKNKNKNKNNLLNVKNIVNIPPQNLFFDFLN
                     TLLVKFQLLKLESNDIVITFVPSKGLDKVFSSYNDLIYYCVHDSNHQSYHARNKKYEK
                     LLVKRSSLTFCDNEIVLNRLAEGSKYINILEKEALFRQDSKFYLVPPPVPNEFYNIAK
                     RNKKFDLVYFGSIHKDININLILNLSIKYSILIISYQNFKILPLHNITFVSGTPNIEI
                     PVKKISLAKAILFPYVNSEFMNSISPAKLYQSIATSMPIYCSNKNLCEKYNLNPVEDI
                     GNDTLIPKNIIHRNINIEDFREKNILSKIELIIRKNLGER"
     CDS             8018..8833
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99816.1"
                     /translation="MDNVAVIMSVYKNDTLEPFKISVNSILNQSYKNIDLYIYRDGIV
                     SGKIQNYLNLLSNEKSISIAKKEYNNGLAHALNELIDIIIAKNKYIYIARMDADDISR
                     NKRIEKQVDYLSKNRDIDVCGTSCKEFGASFALKEKHLPKMHQDLLEFSITRCPFIHP
                     SVMFRTAIFQNGIRYPTNTNLTEDMALWFYLLSKGYKFSNINEILLEYRINEDTLKRR
                     SGLRKALSETRIRFYYMFKLNKVTLKLTILLFFRFLFHLLPNFAIKIIYRKMR"
     CDS             8883..10049
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99817.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             10061..10564
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99818.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(10691..11668)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99819.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLVINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVDRSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKKLLERKVLVTDFSSFLIKISARWYFLIRKITKKAKK"
     CDS             complement(11678..12235)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99820.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYINKMNFEINKYC"
     CDS             complement(12238..13071)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99821.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13171..14184)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99822.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(14184..14441)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5047 a   2373 c   2195 g   4826 t
ORIGIN
        1 agtgaatacc gtcaaggtgt tatgcttgtt gtacttcaca caggaaagcg gtttgttgta
       61 cttctccccc tgcgggcggc ccatgaaagt gaataccgtc aaggtgttat gctagagcaa
      121 catatagaag ccgaactagc gcaagatccg gctaacgatc tgctatggtg gcggcgatta
      181 atccgcgcca ttgataaatg ggcgccacct ggtcaacgtc ttattattgt caccagtgaa
      241 ggtcgtatta ttggtgctca acgtaatgaa atacaagttg tcagaaactt tatgggacaa
      301 tctgataacg cagaccatcc gaaaaagaaa aaatacggtc gctctgagat gttaggtccc
      361 ttttccatta gagatggtga agatcactac caactttact tagtgcgccc atcaagtagc
      421 ccgcaatctg attttatcaa tttattattt gataaaccgc ttttattact gatattcacc
      481 atgcttatca gtacaccgct actggtttgg ctctcttgga gcttggcaaa accggcccga
      541 aaactcaaaa atgcggcgga tgatgtggca aaaggcaatc tgcgccctca ccctgagttg
      601 gaaactggcc cacaagaatt tttagcggca ggcaccagtt ttaatcagat gatcagcgca
      661 ttagagcgta tggttgaagc gcaacaacga ttgatttctg atatctccca tgagttgcgc
      721 accccattaa ctcgtttaca gcttgccagt gcattactac gccgtcgtag cggtgaaagt
      781 aaagagctag agcgtattga aacagaaaca cagcggctag atggcatgat caatgactta
      841 ttagtgcttt cccgcaacca gtataaaaat gagttattac gtgaaaccgt aaaagcgaat
      901 gagctttgga acgatatttt agataatgcg aaatttgagg cagaacaaag caataaaacc
      961 ctaacagtaa ccgccccacc aggcccatgg acaatttatt gcaatcctta ttcattagcg
     1021 agtgcctttg aaaatatcgt tcgcaatgcc ttgcgttatt cacattctcg tattgaagtg
     1081 gcctttaccg aacaaaatca gggtatcact attattgttg atgatgatgg cccaggggtt
     1141 agcccagaag atcgcgagca tattttccga cctttttatc gtacggacga agcacgtgat
     1201 agagaatccg gcggtacagg gctgggactg gcaatagttg aaaccgcaat tagtcaacat
     1261 cgaggccatg ttaaagccga tgatagccca ttagggggat taagagtgga gatttggttg
     1321 ccgagaggga gtgtggtaaa taaatcacaa cacgcttaat tcagtaataa aattactata
     1381 ttaaattatt ctgccatcta ttgaagaata ggtggcatat tcttttcatt cccatcttta
     1441 tagatatttc agaatgatct tgctaattca atcgatattc cgatatttat atatacctaa
     1501 tcaatgttag aatacccatc aattacttga cccgcgggtt tgaagagtct aatctcagag
     1561 cggttttgcg tactgataaa gtatacttat ataataaaac tcttatatta cagatagata
     1621 agagattggc gagaaatttt tggcgctctg ggggccgtaa acccagtgcg gtagctttat
     1681 ttaaaccatc aaatataaaa attaactcta gtgaaaaaat gaatgaattt attccccctt
     1741 ctataagcat agtgattcct gttttcaacg tggaaaaata tatcggtgaa tgcttagact
     1801 ctttattatt acaaacattt gataaatttg aagtgatttg tattatagac ggatctactg
     1861 ataatagtga aaacataata aaatctggaa aatatcaatc attaaacctt aaaattatta
     1921 atatacctaa cagtggacaa tctatagcca gaaacattgg aatacataat gctactggag
     1981 aatttatatt atttttagat agtgatgata aaataaggaa tgatacatta aaaaaatgta
     2041 ttgaagaatt agaggaaaat gaattagatg gtgttttttt tgaaggacaa gcattttcta
     2101 acactttaaa aaaaaataaa atgaaaaaat ataactataa tagacccatt ataaatagaa
     2161 ttatagatgg caaggaatta tacaatatat ctatagaatc acatcttttt acagtacaac
     2221 catgctgtta tatagtaaaa aaaaatataa taaaatcaat aaattttata ccgtcagtct
     2281 accatgagga taatgcatat actattgagt tactattttc aggtaaatta aaaaaaatta
     2341 aaataataaa tgaaaaattt tttctacgtc gtctaagact aaactcaaca atgacttcaa
     2401 taaaaaatat taaacataca aaaggatatc tagcttcaat aaaaatattt tcaaaaataa
     2461 taaaagagag taatgataaa agaacaaaaa aaaatctatc attatactta gaaaagctat
     2521 ttcttggtgc aattattagc aattcatctt gtacaacaac ggacaaaatt actaatctaa
     2581 aagatgaatt attaaaaata atatcagata taggtatcaa aaatgttaca ttaaaaacta
     2641 aaacattacc ttattttatt ccttattttt cgctaagaaa aaatctcatc aaaaaaataa
     2701 ggtcatttaa atgtcagtaa taaataatgc aaaatgggta actgtatctc agctgtttag
     2761 aataataact caaattataa gtattactgt tttaacaaga tttattgaac cttcagagtt
     2821 tgggattctg gctttagcaa ctattataac taactttgct ttagtaataa aagatcttgg
     2881 cttatcctcc gcaataatac aaaaaaaaga attaacaaac gctctaaaaa acaccgtttt
     2941 ttggctcaat attttatttg gtttttcact ttatattcta attattatat tatctaaacc
     3001 aatatctaat tactacgaac aaaataaact gatcaatgtt ctttggctac ttggtacaat
     3061 atttccgatt tcaagcatta gtgcattaca tcaagcttta ttagaaagag actcaaggtt
     3121 caaaataata gcaaaaattg aaataatatc ttcattgttt ggcgttgtta tagccttaat
     3181 tcttgcaata aataactatg gtgtatatag catagtatat caactaatac taactcaact
     3241 aatatcgtct attttgatcc ttataaataa gaaatgggta ccaagcttta cttttaataa
     3301 aaaacaaatt aaatcaatat tagagttcag tagtaatata actatattta atattattaa
     3361 ttatttttca agaaatgcag ataatataat tgttggttct acattctcta ctacaattct
     3421 aggagtatat aacttagcat ataggataat gctattccct cttcaaagtg tcacattcat
     3481 agcaaataga tctttatatc ctataatgag tcgaaaacaa gaaaataaca aagaattaaa
     3541 aaagttatat ttaaaagcga taatggtagt aatgttaata acaacaccaa tgatgatggg
     3601 attagcatat cttagggccg aatttgtaga tatatttttt tcagaaaact gggagtatct
     3661 ttctgaatta ttattatggc tttcacttac aggaattcta caatctgctg taagtactac
     3721 aggtgcagtt tttatggcta aaaatagaac aagaattcta cttctcctcg gaatttttgg
     3781 gactactcta aatttaattg cattttttat tggttcacaa ttttctataa tatttttcgc
     3841 tcaatgctat tttattgcaa atcttataaa tttacttcca cctctatatt gtgcagtaaa
     3901 attattagga ggcggtatta atgatatact taaaattcta ttttcgacct tattaaatag
     3961 tattttaatg ataataatca tatatatttt taattatcta ttctacattg aaaatgttta
     4021 ttttagattt tctataaata tttttattgg ttttttatct tatataataa tattatttat
     4081 attaaataat gaagctaaaa atataattat gagcactcta aaaataaaga ggtaatatat
     4141 gattaaaaat atcatttttt ttatattgaa ttttttcagc caaataatag cttattttta
     4201 tatactaaaa tggaaattag gatttaacta taaagatcaa gctatttcaa tctctgttat
     4261 caaaggaaag cttggataca aagttagaag taatttttat caaagaacat taaataaatg
     4321 tggaaataaa ttaagtgttc attatggtgc atatattgcg taccctcaaa cagtcatagg
     4381 caataggtgc agtattgaag aatatagtgt tataagtctt gctgaaatag gaaacgacgt
     4441 aataatcgca tcaaatgtag tactgttaag tggaagtaaa catcatgaaa tagacgactt
     4501 aagtttaaaa tttcatgatt ctaatataaa gatgacagga ataacattag gagataatat
     4561 ttggataggt gtaaattcag ttattatggc caatattgct tcgggaacaa ttattggtgc
     4621 agggagtgta ctaactaaat caatagttat agaaaattgt atttatgcag gtatacctgc
     4681 taaatatgta aggaatagag gtgttctttg aatatttcta aatttaacat ttttttatta
     4741 attacaatat tttttttaat attattttcc ccttctattt tattttttgt ttcaggacta
     4801 actttaatta ctattgcatg tagtaagcca ttcaataaaa aagaatattt atttttttgt
     4861 aatttaatca tctttttatt cggtttaata ttatatgttt tatcactccc aaacttcata
     4921 ttattttcag atgacttcag tagctactat aataattttc tatatttctt agattctaat
     4981 tataaaaatg gttttaatga atataactat gaatttttat ttccttcttt aaacctagtt
     5041 ctaagcaagt tcatttatgg gaaattccca taccttatga agttaggtca tataataatt
     5101 aacttattat tattatttat tacaattatt tatgtatcaa aaaaagaaaa aataaaaata
     5161 aacgacttcc tattattcac atgctgtatc atgatatttt ataaacttcc acaacaaatg
     5221 catctaatgc gccaaggata tgcctgctat tttattataa tggctttatt ttctaataaa
     5281 atttgttcta aattaatatt tattattatt ggctcttttt ttcacttatc aacacctata
     5341 atttatttaa taataaatgc attaagtaaa attaataaca aaaaaaaact aatagcttac
     5401 actttgttat tcgcatctac atttttacta ttttattatt tatctaaaca aaaaatattc
     5461 tttcactttc cgctattaaa taaattgaac tatattataa attatattag ttctgattct
     5521 catgtgaaaa aatcagtttt agaaacaata aaatcattat tttattttta tccattattg
     5581 attttactaa tcatttataa tataaaaaaa atacatatta aagaaggcta ttttattata
     5641 attagtactt tgtttttaat atctttctct tttattcctg ggtttggaat aagagtttac
     5701 atgccgatac tttcgatttt cttagggtat tattattata aattcattaa acatttaaat
     5761 aataagttta aagctttatt gcttttttgt atatttatat ttataaatat aaattggtta
     5821 ttattctctg agatttatta ttaccgttat cctattttca gcagtgaacc actatattac
     5881 cttaattcat tgatcgaaga acaagaaaaa atatgtcgag aatgtcttcc tcaacaagta
     5941 gagataaaaa ataaatatag gtaatttatg ccaaacattt ctgttgtaat tccatactat
     6001 aatgatagta aacatataga acaatgtatt actagtgtaa tatcccaaac attgacgcca
     6061 ttagaaataa taattataga tgattgctct attgacactg atggcttgag gctaatatta
     6121 aataaattta atttttttaa tattaaatta ttcagaaata atgtgaatat gaatggtgca
     6181 tattcaagaa ataggggaat acacgaagct aaaggagact tcattgccct tttagatgct
     6241 gatgattttt ggagtatgga ccatctagaa aaaaattata attttatatt caaagaaaaa
     6301 gcagattttg tctactccca atgtaataga aataaaaaaa ataaattaat aaaaagaaaa
     6361 gttaatgata ttcatcatat agttggcaat atagaaaata tcattttata ttctccccct
     6421 cagactaact catttttctt ttcatcaaag atatccccat taatatgctt tgatgaaaaa
     6481 ttaaaaagac atcaagacta tcaatttttc ttagatatta ttagaaataa aaatataaaa
     6541 gttagttatt tagatcaata tacaacgtat tatagatatg ctaatactaa taaagaaaac
     6601 attgattact catcaatatt taatttttgg aataaaaaca aagaaaaaat ggataagtcg
     6661 ctattaaaga aaaagttgtg gaatataatt gccggtggat taagaaatag aaaaataaac
     6721 cctgaaacaa tcctatttaa taaagattta gacaatatct tctataatga taagttttat
     6781 aactttacaa aaaaaattaa aaataaaatg attaatcgaa taattataaa tatttatttt
     6841 tatattgtta ttgataggtt caaaaaatta ctacatttta tagggtaagt ttcatgaaaa
     6901 aaagaaaaat tcatatatat agtgatatta attatcatag ctatcaacat attaatcaaa
     6961 tgttatatcg tcaactttct acttcaaata aattttatac aatctttctt caaagattta
     7021 taatgaatag tgtaagatgg aaatatgtgt taaaattttt cagtgcaaca atagataaaa
     7081 taaaaaatcc acataataaa aataaaaata aaaataaaaa taaaaataaa aataatttac
     7141 ttaatgttaa aaacatagta aatattcctc ctcagaatct attttttgat tttttaaata
     7201 ctttattagt aaaatttcaa ttgttaaaac ttgagtctaa tgacatagta attacttttg
     7261 tacctagtaa aggtttagat aaagtatttt cttcatacaa tgatctaata tattattgtg
     7321 tacatgacag taaccatcaa agttatcatg ctagaaataa aaaatatgag aaattattag
     7381 ttaaaagaag ttctttaact ttctgtgata atgaaatagt tttaaacaga ctagcagaag
     7441 gttctaaata tattaatatt cttgaaaaag aagcattatt tagacaagat agcaaattct
     7501 atcttgttcc tcctcctgtg ccgaatgaat tctataatat agccaaacga aataagaaat
     7561 ttgatttagt ctatttcggt tcaattcata aagatataaa tattaattta atattaaatc
     7621 tttctataaa atattctata cttattattt cttatcaaaa ttttaaaata ttaccattgc
     7681 ataatataac tttcgtatca ggtacgccaa atatagaaat ccctgttaaa aaaatctcac
     7741 tagcaaaagc tattcttttt ccatatgtta actctgaatt tatgaactct atttcaccag
     7801 caaaattata tcaatctatt gcaacctcaa tgccaatata ttgttctaat aaaaatttat
     7861 gtgaaaaata taatttaaat ccagtagaag atattggcaa tgatacttta ataccgaaaa
     7921 atataataca tcgaaatata aacattgaag attttcgcga aaaaaacatc ctttcaaaaa
     7981 tagaattaat aataagaaaa aatcttggtg aaagataatg gataacgtag cagtaattat
     8041 gagtgtttat aaaaatgata cattagagcc atttaaaata tcggtaaata gcatattaaa
     8101 ccaatcatat aaaaatatag atttatatat ttatagagat ggaattgtat ctggaaaaat
     8161 acaaaattat ttaaatttat tatctaatga aaaatctatt tctatagcaa aaaaagaata
     8221 taataatggt ttagcacatg cattaaatga attaatagac attatcattg caaaaaataa
     8281 atatatttat attgcaagaa tggatgctga tgatatatca agaaataaaa gaatagaaaa
     8341 gcaagtagat tatttatcta aaaatagaga tatagatgta tgtggaacat catgtaaaga
     8401 atttggagct tcttttgctt taaaagaaaa acatcttcca aaaatgcacc aggatctttt
     8461 agaattcagt ataactcgat gcccatttat ccacccttca gttatgtttc gtacagcaat
     8521 ttttcaaaat ggcatacgtt atccaacaaa tactaactta actgaagata tggcattatg
     8581 gttttatcta ttatcaaaag gatataaatt ttccaatatt aatgaaattc ttcttgagta
     8641 taggatcaat gaagatacgt taaaaaggcg gtctgggtta agaaaagcac taagtgaaac
     8701 tagaattcga ttttattata tgtttaaatt aaataaagta acattgaaat taacaatatt
     8761 actttttttt agatttttat ttcacctcct tcctaatttt gcaattaaga ttatatatag
     8821 aaaaatgaga taatatttat tatctctttt taaataaatt tacttatata ataggttttt
     8881 ttatgaaaat taccatctct ggtaccggct atgtcggtct atctaacggg atactccttt
     8941 cccaaaacaa caaagttgtt gcattagata ttaataaaga aaaagtcgaa ctattaaacc
     9001 aaaaaatctc tcctatttct gataaagaaa ttgaagagtt tttagctact aaagagctcg
     9061 actttacggc tactttagat aaagaattcg cttataaaga tgctgaattc gttatcattg
     9121 ctactcctac cgattatgat cctaaaacta actactttaa cacctcttct gtagagtcag
     9181 ttattcgtga tgttttagaa tataatccaa acactactat gattataaaa tcaaccattc
     9241 cagttggttt tactaagcag atgagagaaa aatatcatac cgataatatt atcttctctc
     9301 ctgagttctt acgtgaaggt cgcgcccttt acgataacct ctatccatca cgtattgtga
     9361 ttggtgagca atctgcaaga gcagagaaat ttgctgagct tttacaacaa ggagcattaa
     9421 aaaaagatat tgatgttctt tttactgact ctacagaagc agaagcaatc aaactatttg
     9481 ctaatactta tctcgcattg cgtgtggctt attttaatga actcgatagc tacgcccaag
     9541 cttacggctt aaacgcaaga caaattattg aaggtgtctg tttagatccg cgcattggta
     9601 atcactataa caacccctca tttggttatg gtggctactg cttaccaaaa gatactaaac
     9661 aactgttagc caactacgaa tcagtaccta ataatatcat tggtgccatt gttgaagcta
     9721 accgcactcg taaagacttt attgctgatt ctattatcgc taaatcaccg aaaattgttg
     9781 gtgtttatcg tttaattatg aaatcaggct ctgataattt ccgctcatct tcaatccaag
     9841 gcattatgaa gcgtattaaa gcgaaaggca ttgaagtggt aatttatgag ccagaaatga
     9901 aagaagagac tttctttaac tcaaaactca ttcgtgattt agatgcgttt aaagcaatgt
     9961 ctgatgtcat tattaccaat agaatgtctt ctgcactaaa cgatgttgaa gaaaaagttt
    10021 atactcgtga tctctttggt aacgattaaa actaatttat atgctcaata tcgtcttatt
    10081 tgaacccgaa attccaccga atacgggtaa tattatccgc ctgtgtgcca acacaggctt
    10141 tcatcttcat ttgatccaac cattgggttt tacttgggat gataaacggc tacgccgtgc
    10201 ggggcttgat tatcgtgagt ttgctgatat caaacagcat cacgattatt atgctttttt
    10261 agaaagtgaa ggattaagcc ctgataatgc acaatccccg acaggtgcac ggttgtttgc
    10321 attaaccact aaaggtactc ccgcccatag taatgtaagt tatcaagatg gggattatct
    10381 gctatttggt cctgaaacac gaggtttacc accttatgtg ttggataata tgccaacaca
    10441 acaaaaaatc cgtgtgccaa tgttagcgga tagtcgtagt atgaatcttt ctaatacggt
    10501 tgctgtggtt gtttttgagg catggcgcca attaggctat ccgggggcat tattaagaga
    10561 ttaatttatc tttttaattt ataaccataa aaaatgacct ctagacttta ttgtctcgag
    10621 gtcattttta tgtatatgct ttgtgctagt tttagctttt cttatttttt atctttttta
    10681 tctttcggtt ttatttctta gcttttttag ttatttttct tatcaagaaa taccatcttg
    10741 cacttatttt tattaaaaat gatgagaaat cagttactaa tacctttctt tctaataatt
    10801 ttttcttaaa ttcatttttt tgtattaact cttttcgata ctctttagca ttaaattgtt
    10861 gattaatctt ttcaagtaac cttttacagc gtactacttg ctctactgtt tttttatcat
    10921 tatccaataa taatgaataa tcctctttat tcacgttaat gccaaaatac attaataatt
    10981 ttcgtcctac tctttccgcg gtatcttttt gtatcgcttt atattgcgag cgatcgacat
    11041 ttggatgagt acgataataa agaagtggta caggaagatt atggagctta aattttccag
    11101 caataattaa gttcacccat aaaccataat cttctgcata gaggtctgct ttatcatatc
    11161 caccaacaga taatatatct tgcttacgat agataatggt tggatgcgcc aaggagcaat
    11221 cataaagact agaaatcaca atatcactat gtgtttctgg ataagtatta attctatttg
    11281 gctcttcttg gtagataaaa gcaccaccac aaacactcac ttcaggatgt tgtaacataa
    11341 attgatattg cgcttctagt ctggttgaaa cggcaatatc atcagagtcc attctagcaa
    11401 ctaaatcaca tgaagctaat tgtaatcctt tgtttaaaga agcgatcaat ccgatatttt
    11461 gttcattaat aatcagtttt attctgggat cttttttggc attttctttt aaaatagata
    11521 atgagttatc cgttgagcca tcattaatga caagaaactc aaattcttta aatgtttgat
    11581 ttaatataga atcaatagac tcctgaaggt atttttcatc attgtatacc gacattaata
    11641 cggtaatttt gggcatattg tttttcatta tctattttca gcaatattta ttaatttcaa
    11701 aattcatctt attgatatac cgacaccctt ctcccacatc ctttaatact aatgcatggg
    11761 cgccaataat cgcattatca ccaatattaa tctctccacc aataatggat gaattacagc
    11821 caatataaac atgatctcct acttggatat tggcttttcc ccctttctct tttacgccaa
    11881 tagtcacccc ttgcataata gtgacatatt cccccagttg gcagtaatta gtcagtacaa
    11941 tgccaatcgg gtgtggtaaa gaaaggcctt taccaatacg agtttttaac gagatatcat
    12001 tacagtattt acggttattg ctagcaccga tccgaatacc taatttatgt aaaaagcgat
    12061 ggggagagaa ataaaaatac tgtgctaccc gccaccaaaa taggtaacgg gaacgctgac
    12121 agaaaaatac tcgatgaaat agccttaacc aactaaaggg ttttttatca ccaatatatt
    12181 cagcctgtaa gcaatctttt agttcagcaa gggtaacctt agcttgggca tgcatagtta
    12241 aataccatca ccacactcaa agccgggtac aacaccatta aagtgttgat ccatatcgaa
    12301 tgacggtttt tcactatcag gacgaccgac aatgcgggca ggcacccccg ccaccgtagt
    12361 atgagccggt acttcatgta atactaccga gccagcccca attttggcac cacgaccaat
    12421 ctcgatatta ccaagaattt tagcccccgc gccaatcatt accccttcac gcactttcgg
    12481 atgacgatcg cctcctgttt tacctgttcc ccctaaggtc acagattgta aaatggagac
    12541 atcattttca actaccgccg tttcaccaat cacaatacct gtcgcatggt cgagcataat
    12601 accgtgtcca atacgtgccg ctgggtgaat atccacccca aaggtgactg atatttggtt
    12661 ttgtagataa atagctagcg cttggcgatt ttgttgccat aaccaatggg caatacggta
    12721 ggcttgtagt gcatgaaacc cttttaaata gagcaatggg gtagaatatt tatccaccgc
    12781 aggatcacgt aggcgtaccg cagagagatc acaagcggcc gactcaatca ttgaactatc
    12841 gtgacgataa gcctcttcaa cgatttcgcg tactgcaatg gctggcatag tctgagttgc
    12901 cagcttattg gccagcatat agcttaatgc actacctaaa ttttcgtgtt tgagaagtgt
    12961 cgcatgaaag aaactggcta acattggctc acaatctgcc aatgcacgcg cttcgctttt
    13021 aatatgatcc cagactcttg ttagttcttc tagcgacata ctcactctca ttttttacac
    13081 caaagcctaa gtaaatactt aggctttgaa ttttcaaaca aatggatagt taaaacagat
    13141 accctaagaa tattatttaa aataaactga ctacaggctg ctgtctatct catctttggt
    13201 tgctcgccct aataaagcca atgcagcctc ttttacatct ttatgttgat ataaaatttg
    13261 gtaaatctgt tcggtgattg gcatttctac ccccacttgt tcggctaatg cgcgaacttc
    13321 tttggtgtta cgataacctt cgactacctg gccaattttc tcttgggctg tatcaacatc
    13381 taaaccttga cctagcatca taccaaagcg acgattgcgt gattggttgt cagtacaggt
    13441 taaaactaaa tcacccaaac cagccatgcc cataaaagtt gccgcatctg cacctaaggc
    13501 tttacctaag cggctcattt cggccagacc acgggtaatc agtgcggtac gcgcattagc
    13561 gccaaatccc ataccatcag acatacccgc accaatagca atcacgtttt taacagcgcc
    13621 ccccagttgc acaccgataa aatcagggtt tttatagact cggaagcttt taccacaatg
    13681 aaatagctgt tgtaactgct ctaaaaagag attgtctgtc gaggccactg caatcgcggt
    13741 gggcaaaccc gccgcaagct ctttagcaaa agtagggcca gataatactg cgagcgggat
    13801 ttcattacct aatacttcac gggcaacatc ttgtaataag cgaccagtat gtgcttcaag
    13861 accttttgtc gcccaaacaa cacgtgcatc ctgacgtaaa aagggtttga tttgttgcag
    13921 tacttcacca aacacatggc ttgggatcac cacaagaata ttacggctcg cttcaatcgc
    13981 tttttgcaaa gaagcttcca tatataaact atcaggaaag gaaacatcgg gcagaaaggc
    14041 ttgattacag cgcgcttgtt ctaatgccgc aacgtgcttg ggatcatgcc cccacagcac
    14101 aacatcgtgc ccattacgcg ctaaggtaat cgctaaagcg gtgccgtatg aaccggcacc
    14161 gataactgtc atagaagcgt tcattaagct tcttcaactc ctgagtttgc agcatcctgt
    14221 tgctgttgtt gctgtaagta gttcataaac aacgcatcaa agttaacagg tgctaagttt
    14281 aattgtggga aagtaccacg acccactaag ttggtgatac attcacgtgc ataagggaat
    14341 aatacgttcg ggcagtatgc acctaagcaa tgtgctaatt gtgtaccttc gataccttca
    14401 acagtaaaga tacctgcttg ttgtacttca cacaggaaag c
//
