LOCUS       KY710720               11904 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain ATCC 49990 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710720
VERSION     KY710720.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="ATCC 49990"
                     /serotype="O52"
                     /culture_collection="ATCC:49990"
                     /db_xref="taxon:585"
                     /collected_by="American Type Culture Collection, USA"
     misc_feature    1..11904
                     /note="O antigen gene cluster"
     gene            11..853
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1184..2026
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99842.1"
                     /translation="MINPLSNRKVAFIMSVYKNDKISFLKESLDSILIQTHNNWILRI
                     KIDGPVKPSIIELIEQYKNDNPSYDIKFFYRNENKGLAKSLNELIDYIIKYDNDVTYI
                     ARMDADDINFSDRLEKQVLFLEENKTVDLIGSSCQEFGTNFSIIKKMETEHNKLVDNI
                     FKKCPFVHPSVIFRKSIFLNGFRYPIDTVLSEDLAFWFLLIENGYKFSNIEKPLIHYR
                     TSDDMLKRRKGLKKAFNEIKIKTFYMKKLKMTNMRNIIFIFSYFLIRISPTFFVRYLY
                     RYLR"
     CDS             2124..3194
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99843.1"
                     /translation="MFYIFLSIIELIKNRTFVNKKIFILIYPILFSLIVILYRALFNE
                     SIYLKDILLPFIQIKMLLFLFSSLILWKYLKKESLFKFIIISVIIQLIIATLQKLNIP
                     LFSNGLFYNYAVYNAMSNVYYGGNIDHIINTHLKVTFRPIGFIGSPTILATYFLVIFS
                     LWNIMNFSKKKVILIGYSLLLCFSKITILSYIIYNFIVNLLKTKSIKKILIIITSGVS
                     SLLLFILFLITNDRIYNYFIKSIDGSDYGVTHRKSVLDYISNLNIESILFGIGGEPPF
                     IFDSGTLLTIFRFGIVYLLLIYYSYYILLKIFSVKNSHLYLFLIYLFADMTIGVFHNQ
                     MFFYLISFSILYSKYIKEKNEI"
     CDS             3184..4374
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99844.1"
                     /translation="MKYKLWKYIDQFLFALINIGTILFISKFINEHEASNYIYVTTFT
                     SLSLVVNAAFIISPTWYYCTNIERLKEYHSFNLLLLILSSIVVSIFIYTLMREKNLLL
                     SINIFLLCLLFPLFDYLRRILYILKDEKKAGISSLLLVCLTLVSYVILKFIYNIEEYI
                     YYISYLILSLFTSSLFILIKLTLKYEINFHIKTLFSPQKLKNYISLGKWSALSMLCFW
                     ISTQGIFIIFNNHISNQAFVISKVCLSFSSIIAIFFSAIENNLMPSLRKNIINKDFVN
                     IEKIKVKYFKNGLIISILFTFISIVSYFLLYDFNYTALFILLMLCFYQAFSGLFKIYA
                     YLLKSINNYNLLFYINLFCTIVILTLASILLDKNNYLISITVLIFGISTSILYKIALH
                     FHLR"
     CDS             4380..5357
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXY99845.1"
                     /translation="MINLNHINTKIGVLDTSICSMNVGDFIIMDSCYKVVSDLFPNAQ
                     KIHFPTHERITRVGLKRQKEIELNFLCGTNCLNSSMFLHRQWNVGYLNSILMKPIVSL
                     GVGWGNYQNAPDLYTKILLKKIFSNQHLLSVRDSYTEDKFKLAGLKNVINTSCSTMWT
                     LTPEHCELIPKNKANDVVFTLTDYRKNKEFDLTLIRALKSTYKNVYFWAQGSQDYNYF
                     MSFNNEINDINVIPANLKSFDSLLDNHISLDFIGTRLHAGIRALQKGKRTTIISVDNR
                     AEEKKKDFNLNVIPRNWDISNYIDFFNNSYTTNIKIPLENIAKWRSQFE"
     CDS             5350..6411
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99846.1"
                     /translation="MNKRNILFIISSLEKGGPVNQLFELLSKINENSFNIILLSLSSL
                     DKEKSRYRDFLKLNIELMSLNYDGYNFFKINTSLKKIILDKHIDVVISQGFRSDISLP
                     KLISQKKISILHNYIYPDYRHEYGLIKSIVMSLLHISALKKVDTVISVSQSVSEYIKS
                     KYNLTSKYIRNGVQEKKEKNTLFSEKKIINLVYVGNLDKRKNVKALLKSFNILNGNFR
                     LRIIGSGPEEENLREQYLFNSNIVFEGYSDHVDDILYESDIFISASTFEGLPMAVLEA
                     LSVGIPCILSDIPPHNEIINLNKCFGEIYNFNKDNNLDEKIQSCIKKNGQDIRNNFIK
                     YLSSNRMAKEYVDIIKKQI"
     CDS             6481..7647
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99847.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSRLVRDLNEFKSISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             7659..8162
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99848.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(8250..9221)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99849.1"
                     /translation="MKNIMPKITVLMSVYNDEKYLQESIDSILNQTFTEFEFLIISDG
                     STDNSFNILKENAEKDPRIKVIINEENVGLIASLNKGLKLASCDLIARMDSDDIAIAS
                     RLEEQYQFMVDNPNISVCGGGFIYQEDPDRINIYPETHTDIVISSLYDCSLAHPTVIY
                     RKLDILSVGGYNKEDLYAEDYGLWVNLIITGKIKLHNLPIPLLYYRTHPTVERSKYKE
                     IQKNTAEKLGRKLLKYFGILINEEDYALLLDNNKKTKEQVVRCKALLEKIEQQFNAKE
                     YSEELKLKNSFKKKILERKVLVNDFSSFLMKLRARWYFLMRKFNKKK"
     CDS             complement(9232..9786)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXY99850.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSENRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYINKMSFEINKYC"
     CDS             complement(9786..10619)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99851.1"
                     /translation="MRVSMSLEELKRVWDHIKDEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(10698..11714)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99852.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(11714..11902)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4066 a   2006 c   1838 g   3994 t
ORIGIN
        1 cacgccttaa tgccggtttg gtgggtgccg accgttggtc ggcacgcctt cagccatcaa
       61 tgcgagcgct gcaccgcgta gcgggccagg ccgcgcaggg cagccacagc gtcgttgtcg
      121 cccaggccgt cgagcgcacg ctccgcactc tcggcaagaa cgtatggttg aagcgcagca
      181 acgcttaatt tctgatattt ctcacgagtt acgaacacca ttaacacgct tacaattagc
      241 gagcgcattg ttacgtcgtc gtagtggaga aagtaaagag ctagagcgta ttgaaactga
      301 gactcaacgc cttgatggca tgattaatga tttactggtt ctttcacgca accaacataa
      361 aaacgagtta ttacgtgaaa cagtaaaagc caacgaactg tgggatgata ttttagataa
      421 cgctaaattt gaggctgaac agagtaataa gacgctacaa gtaacaacac ctccgggagc
      481 atggcctatc tactgcaacc catattcact tgctagcgca tttgaaaata ttgttcgtaa
      541 tgctctacgt tattcaaatt ctcgcattga ggttgccttt actgaacaaa accaaggtat
      601 cactattatt gttgatgatg atggtccagg tgttagtcct gaagatagag agcatatctt
      661 ccgacctttc tatcgcactg acgaagcaag agatcgcgaa tcaggtggta ctggcttggg
      721 attagctatt gttgagacag cagtaagcca acatagaggc catgttaaag ccgatgacag
      781 cccattaggt ggattgaggg ttgagatttg gttgccgagg gctggcgctg gtaataagtc
      841 accaacagct taatagagta attaaactgt tgtatcaaaa tatactgcca tctattgaag
      901 aataggtggc atattctttt tgttaacgtt tgttgatgta attcagaatg atctggctga
      961 atcgaccgtt attccagtat ttatatatct tcaatcaatg ctaaaatacc cggctatcac
     1021 ttgaccgtct ggtttgaaga gtctactctc agtgcggttt ttagcatatt gataaagtat
     1081 gcttatacaa caaaactctt atattacaaa aagataagag tttttcgaga taaattttgg
     1141 cactctggga gccataaacc cagtgcggta gctatggaaa aatatgatta atcctctatc
     1201 aaatagaaaa gtcgcattca taatgagcgt ttataaaaat gataaaattt catttttaaa
     1261 agaatcatta gatagcatct taattcaaac acataataat tggattctaa gaataaaaat
     1321 agatggccca gtaaaaccat ctataattga actaatagag caatataaaa atgataatcc
     1381 ttcttacgat ataaaattct tctacagaaa tgaaaataaa ggattagcaa aatcattgaa
     1441 tgaactcatt gattatatta taaaatatga caacgatgta acatatatag caagaatgga
     1501 tgctgatgat attaattttt cagatagatt agaaaagcaa gtcttgtttt tagaagaaaa
     1561 caagactgta gatcttatag gaagtagttg tcaagaattc ggtacaaatt tttctattat
     1621 aaaaaaaatg gaaactgaac ataataaatt agttgataat attttcaaaa aatgtccttt
     1681 tgttcatcct agtgttattt tcagaaaatc tatttttctc aatggattta gataccctat
     1741 agatactgta ttatcagaag atttagcttt ctggttcttg ttaattgaaa atgggtataa
     1801 attttctaat attgaaaaac cacttatcca ttatagaaca tctgatgata tgctaaaaag
     1861 aagaaaagga ttaaaaaaag cgtttaatga aataaaaatc aagaccttct atatgaaaaa
     1921 attgaaaatg actaatatga ggaatattat ttttattttt tcttatttct taatcagaat
     1981 ctcaccaacc ttttttgtca gatatttata tagatactta agatgactat aacatttatt
     2041 tattgtttta tttttttttc tgctatattt ttacctacaa ttagaatatc agggatgccc
     2101 atactaatac ctgatattat aatgtgtttt acattttttt atctatcata gaattaataa
     2161 aaaacagaac attcgtaaat aaaaaaatat tcattcttat atatccaatc ttatttagct
     2221 taattgtaat tttatataga gctttattta atgaaagtat ttatttaaaa gatattttat
     2281 taccctttat tcaaattaaa atgctgttat ttttattcag ctctttaatt ctatggaaat
     2341 atttaaaaaa agaatctctt tttaaattta ttattatttc cgttataatt caattaataa
     2401 tagctacatt acaaaaacta aatattccat tattttcgaa cggattattt tataattatg
     2461 ctgtctataa tgcgatgagc aatgtttact atggtggaaa tattgatcat ataataaata
     2521 ctcacttaaa agttacattt agacctattg gatttattgg ttcgccgact attttagcca
     2581 cttactttct agttattttt tctctttgga acattatgaa ctttagcaaa aagaaagtaa
     2641 ttctaatagg atattcatta ttactttgtt ttagtaaaat aactatttta agttatatca
     2701 tctataactt catagttaat ttattaaaaa caaagtctat taaaaaaata ttaataatta
     2761 ttacaagtgg ggtaagttca ctattattat ttatcctttt tcttataaca aatgatagaa
     2821 tttataatta ttttattaaa agcattgacg gttctgatta tggtgttact catagaaaat
     2881 ctgttttaga ttatatatca aatttaaaca ttgaatccat tttatttggc attggtggcg
     2941 aaccaccatt tatctttgat agtggcacac ttctgactat ttttagattt ggtatcgtat
     3001 atttattatt gatatattat tcatattata tattattaaa aatattcagc gttaaaaaca
     3061 gtcatttata tttattttta atctacctat ttgcagacat gacaataggt gtattccata
     3121 atcagatgtt tttttactta ataagcttca gtatcttata cagcaaatat ataaaagaaa
     3181 aaaatgaaat ataaactctg gaaatatata gatcaattcc tatttgcctt aataaatatt
     3241 ggtactatat tgtttatatc taaatttata aatgagcatg aagcaagtaa ttatatttat
     3301 gtgacaacat ttacatcatt atctttagtt gtgaatgctg cattcataat atcaccaact
     3361 tggtactact gtactaacat tgaaagatta aaagaatatc actcttttaa tttattatta
     3421 ctaatactat ccagtattgt agttagtatt tttatatata ccctaatgcg tgaaaaaaat
     3481 ttactcttat ccatcaatat atttcttctc tgtttgttat ttccattatt tgattatctg
     3541 agaagaattt tatatatcct aaaagatgag aaaaaagcag gaataagttc attattactt
     3601 gtatgtctta ctttagtatc ttatgtaata cttaaattca tctataatat agaagagtat
     3661 atatattata tatcttattt aatattatca ttatttactt catctttatt tatcttaatt
     3721 aaattaacac taaaatatga aataaatttt catataaaaa cgttattttc tccccaaaaa
     3781 ttaaaaaact atattagctt aggtaaatgg tcggctttat caatgctttg tttttggata
     3841 tcaactcaag gtatatttat tatttttaat aatcatatat caaatcaagc ctttgtaatt
     3901 tctaaagttt gcttaagctt ttcaagtatt attgctattt ttttctctgc gatagaaaac
     3961 aatttaatgc cctcattaag aaaaaatatt atcaataaag attttgttaa tatagaaaaa
     4021 ataaaagtaa aatactttaa aaatggatta attatatcta ttttatttac ctttatttct
     4081 attgtttctt atttcttatt gtatgatttt aattatactg cattatttat tcttttaatg
     4141 ttatgttttt atcaagcatt tagtgggttg tttaagatat atgcatactt acttaaatct
     4201 ataaataatt ataacttact attttatata aacttattct gtacaatagt aatattaact
     4261 ttggctagta ttcttttgga taagaataat tatttaatta gtatcaccgt tttaatattt
     4321 ggaatatcca cctctatttt atataaaata gcattacact ttcacttgag ataattttaa
     4381 tgataaacct aaatcatatt aatacaaaaa taggtgtttt agatacaagc atttgttcta
     4441 tgaatgtcgg cgattttatt ataatggatt catgttataa agtagtatct gatttattcc
     4501 caaatgctca aaaaattcat tttcctactc atgagaggat aaccagagta ggtctaaaaa
     4561 gacaaaaaga aatcgaatta aattttttat gtggcactaa ttgccttaat tcatcaatgt
     4621 ttttacatag acagtggaat gtaggatatt taaattcgat attaatgaaa ccaattgtat
     4681 ctcttggtgt aggttgggga aactatcaaa atgctcccga tttatataca aaaatattac
     4741 taaaaaaaat attttccaat cagcatttat tatcagtgag agatagctat actgaggaca
     4801 aatttaaatt agctggttta aaaaatgtta taaatacatc atgttcaact atgtggacac
     4861 taactccaga acactgtgaa ttaattccca aaaataaggc taatgatgtt gtatttactt
     4921 taacagatta tagaaaaaat aaagagtttg acttaacttt aattagagca ttaaaaagta
     4981 catacaaaaa tgtgtatttt tgggcccaag gctctcaaga ttacaactat tttatgagtt
     5041 tcaataatga aataaatgat ataaatgtaa taccagctaa cttgaaatca ttcgattcac
     5101 tacttgataa tcatatctct ttagatttta ttggcactag acttcacgcc ggaattaggg
     5161 ctctacaaaa aggtaagaga accactataa tttctgttga taatagagca gaagaaaaaa
     5221 agaaagattt taacttaaat gtaataccta ggaattggga tatatctaat tatatagatt
     5281 tctttaataa ttcttataca acaaacatta aaattccatt agagaatatt gccaaatgga
     5341 gatcccaatt tgaataaaag aaatattctt tttattatat cttctctgga aaagggtggt
     5401 cctgtaaatc aattatttga gctactatca aagataaatg aaaatagctt taatataata
     5461 ctactttcat tatccagttt ggataaggag aaatcaagat atagagattt tctaaaatta
     5521 aatatagaat taatgtctct taattatgat ggatataatt tttttaagat aaatacctct
     5581 cttaaaaaga taattctaga taaacatatc gatgtggtta tttctcaagg atttagatct
     5641 gatatttcat tacctaaatt aataagtcaa aaaaaaatat ctattcttca taactatata
     5701 taccctgact atagacatga gtatggctta attaaatcga tagtaatgtc actacttcat
     5761 attagtgcat taaaaaaagt agatacagta atttccgtgt ctcaaagtgt cagtgaatat
     5821 ataaaatcaa aatacaactt gacttcgaaa tacattagaa atggtgtaca agaaaaaaaa
     5881 gaaaaaaata ctctatttag tgaaaaaaag atcattaact tagtttatgt aggcaattta
     5941 gataaaagaa agaatgttaa agctctttta aaatcattta atattctgaa tggtaatttt
     6001 agattacgta ttataggttc agggccagaa gaagaaaact taagagaaca atatctattt
     6061 aatagtaata ttgtttttga aggttacagt gatcacgtgg atgatattct atatgaatca
     6121 gatattttta tatccgcatc tacttttgaa ggattaccaa tggcggtatt agaggcttta
     6181 tctgttggaa ttccttgtat tttatctgat attcctcccc acaatgaaat tattaatcta
     6241 aataaatgtt tcggtgaaat ttataacttt aataaagata ataatcttga tgaaaaaatt
     6301 cagtcttgta taaaaaagaa tggacaagat attcgtaata acttcataaa atacttatca
     6361 tctaaccgaa tggcaaaaga gtatgtagat ataataaaaa aacaaatata attaatatat
     6421 aattaagtta caaaaatctc atctatatta ttttatttaa atgcttagat aagaagaatc
     6481 atgaaaatca caatctccgg taccggctat gtcggtctat ctaacggaat acttctttcc
     6541 caaaacaaca aagttgttgc gttagatatc aataaagaaa aagttgaatt acttaacaaa
     6601 aaaatctctc ctatttctga taaagaaatt gaagagtttt tagcaactaa agagctcgat
     6661 tttactgcaa ccttagataa agaattcgct tacaaagatg ctgatttcgt tatcattgca
     6721 acacctaccg attacgatcc taaaacaaac tattttaaca cctcatcagt agagtctgtt
     6781 attcgtgatg ttttagaata taatccaaat acgacaatga ttattaaatc aaccattcct
     6841 gttggtttta cgaaagaaat gagagaaaag tataatactg ataatattat tttctcccca
     6901 gaattcttac gcgaaggtcg cgcactctac gataatcttt atccatctcg tattgttatt
     6961 ggtgagcaat cagaaagagc acaaaaattt gctgacctat tacagcaagg tgcactgaaa
     7021 aaagatattg atgttctgtt cactgactcc acagaagcag aagccattaa attatttgcc
     7081 aatacttatt tagcgctacg tgttgcttac tttaatgagc ttgatagcta tgcagaatct
     7141 tacggcttga actcaaaaca aatcattgag ggcgtctgtt tagatccgcg tattggtaac
     7201 cattataata atccatcgtt tggttatggt ggttactgct taccaaaaga taccaaacag
     7261 ctgttagcaa actacgattc tgtgcctaat aatattatta gtgccatcgt tgaagcaaac
     7321 cgtacacgta aagactttat tgctgactca attatcgcta aatcaccaaa aatcgtcggt
     7381 gtttatcgtt taattatgaa atcaggttct gataatttcc gttcttcttc tatccaaggc
     7441 attatgaaac gtattaaagc caaagggatt gaagtggtaa tttatgagcc agagatgaaa
     7501 gaagaaacct tctttaactc aagattagtt cgtgatttaa atgaatttaa atcaatctct
     7561 gatgtcatta ttaccaatag aatgtcatca gtgctaaacg acgttgaaga aaaagtttat
     7621 actcgtgacc tctttggtaa cgactaaatc tattttatat gctcaatatc gtcttatttg
     7681 aacccgaaat tccaccgaat acggggaata ttatccgcct gtgtgctaac acaggctttc
     7741 atcttcatct aatccagcca ttgggtttta cttgggatga taaacgtcta cgtcgtgcag
     7801 ggcttgatta tcgtgagttt gctgatatta aacagcatca cgattactat gcttttttag
     7861 aaagtgaagg cttaaaccct gataatgcac aatcaccaac aggtgcgcgt gtatttgcgt
     7921 taacgacaaa aggcacacca gcacatagta atgtgagtta tcaagatgga gattatctcc
     7981 tttttggacc tgaaacccgt ggtttaccgc cttatgtgtt agacaatatg ccaacacagc
     8041 agaaaatcag agttccaatg cttgctgata gccgtagtat gaacctatcg aatactgtgg
     8101 ctgttgtagt ttttgaagca tggcgtcaac ttggttatcc tggtgcgtta ttaagagatt
     8161 gatctttcga tacaaaaatg accttatagg atttatattt tcaataaggt catttttatt
     8221 tatgcttcca agaacatcac tattccaatc tatttcttct tattaaattt tctcatcaaa
     8281 aaataccatc tcgctctcaa tttcattaaa aatgatgaga aatcatttac tagtaccttc
     8341 ctttctagga tttttttctt aaagctattt tttaatttta gttcttcact atattcttta
     8401 gcattaaatt gctgttcaat cttttcaagt agggctttac accttactac ctgttcttta
     8461 gtttttttat tattatctaa taataacgca taatcctctt cattaatgag aataccaaaa
     8521 tactttaata gttttcgtcc taatttttca gctgtatttt tttgaatttc tttatattta
     8581 gagcgttcta ctgttgggtg agtacggtaa taaagtaatg gtataggaag attatgaagc
     8641 ttaatttttc ctgttatgat tagatttacc cataaaccat aatcttctgc gtaaagatcc
     8701 tctttgttat atccaccaac agataaaata tccaatttcc gataaataac ggttggatga
     8761 gctaatgaac aatcgtaaag gctagagata acaatatctg tatgtgtttc agggtaaata
     8821 ttaattctat caggatcttc ttgataaata aaaccaccac cacacacact gatattggga
     8881 ttatctacca taaattgata ctgttcttct aatcttgaag caatcgctat atcatcagaa
     8941 tccattcgag caataagatc acatgaggct agctttaatc ccttatttaa agatgcaatt
     9001 aacccaacat tctcttcatt gataataact tttattcttg gatctttttc agcattttct
     9061 tttaaaatat taaatgaatt atctgttgag ccatcactaa taatgagaaa ctcaaattca
     9121 gtgaatgttt ggttcaatat agaatctata gactcttgta ggtacttctc atcattatat
     9181 accgacatta atacggtaat tttaggcata atatttttca ttaatatatt ttcagcaata
     9241 tttattaatc tcaaaactca tcttattaat atacctgcac ccttctccca catcctttaa
     9301 taccaatgcg tgagcaccaa tcactgcatt atcacctatc tctatctctc cgccgataat
     9361 cgaagaatta cagccaatat aaacatggtt gccaactcga atatctgctt tgccatcttt
     9421 ttcttttact ccaatggtga caccttgcat taacgtcaca ttctcaccta attggcagta
     9481 attagtcagt acgatcccta tcggatgagg taatgaaagg cctttgccta tgcgagttct
     9541 tagtgaaata tcgttgcaat acttacgatt atttttagca ccaatacgaa tgccaagttt
     9601 tttgagaaaa cggttttcag agaaataaaa gaattgggcg acacgccacc aaaatagata
     9661 acgtgaacgt tggcagaaaa aaacgcgatg aaaaagtctt atccaactaa aaggcttgtt
     9721 atcaccgata tattcagcat gtaaacaagc cttcagctca gagagtgtaa cttcagttaa
     9781 tgccattaaa tgccatcacc acattcaaaa ccaggcacca caccattaaa gtgttgatcc
     9841 atatcgagtg aaggcttgtc actcgtcggc ttaccgacaa tacgggcggg aacacccgca
     9901 acagtggtat gtgcaggaac ttcatgtaat accacagagc cagcaccaat tttggcaccg
     9961 cgaccaatct caatattgcc gagtatttta gcacctgcgc caatcattac accttcgcgt
    10021 actttagggt ggcgatcgcc acaggtttta cctgtacccc ctaaagtgac ggattgtaga
    10081 atagaaacgt cattctcaac aatagcggtt tcaccaatca caatacctgt tgcatgatca
    10141 agcataatgc catgaccaat gcgagcggct ggatgaatat caaccccaaa agtcacagaa
    10201 atctggtttt gtaggtaaat agcgagtgct ttacgatctt gcccccatag ccaatgcgct
    10261 atgcgataag cttgtaaggc atgaaaacct tttagataaa gcagaggtgt ggagtactta
    10321 tcaacagcgg gatcgcgtaa acgaacggct gaaatatcat aagcagctga ttcaatcata
    10381 gaattatcac tacgatatgc ttcttcaaca atctcacgaa cagcaatcgc aggcatagtt
    10441 tgggttgcta gcttgttcgc cagcatatag cttaaggcac tacctaaatt ttcatgcttg
    10501 agtaatgtcg catggaagaa actcgccaaa attggctcgc aatctgccaa acatcttgct
    10561 tcatctttaa tatgatccca gactcttttt agttcttcta gcgacatact cactctcatt
    10621 ctttttacat tgaagcctaa gtaaaaactt aggcttctta tgtttaaaac agaaataaat
    10681 aactatctaa aatagactta gaaaagatta ctgtctatct catctttcgt tgcacggcct
    10741 aataaagata atgccgcttc tttagcgtct ttatgctggt ataaaatttg gtagatctgc
    10801 tctgtgatag gcatttcaac accaacttgc tcagctaatg cacgaacctc tttggtattg
    10861 cgataacctt caacgacctg cccgattttc tcttgggcag tatcaacatc aaagccttga
    10921 cctagcatca tgccaaatcg acgattacga gattggttat cggtacaggt taaaactaaa
    10981 tcacctaaac ccgccattcc cataaaagta gcagcatctg cacctaatgc tttacctaat
    11041 cggctcatct cggcaagtcc gcgggtgatt agcgctgtac gcgcatttgc accaaatccc
    11101 ataccatcag acatacccgc accaatcgca atcacgtttt ttaccgcgcc acctaattgc
    11161 acaccgataa agtcaggatt tttatacaca cggaaacttt tgccacaatg gaaaagctgt
    11221 tgaagctctt cagagaacgt attatccgtt gatgccacag aaatcgcagt gggcaaaccc
    11281 gcggcaagct ctttagcaaa agtaggacct gataaaactg ctagcgggat ttcattacct
    11341 aatacttcac gcgctacatc ttgcaacaag cgaccagtat gagcctcaag tccttttgtc
    11401 gcccaaacaa cacgtgcatc ctgacgtaaa aagggtttga tctgttgtaa tacatcacca
    11461 aaaacatggc ttgggatcac aacaagaata ttacggctgg cttcaatggc tttttgcaaa
    11521 gaagcttcca tgtataaact atcaggaaaa gaaacatcgg gcagaaatgc ctgattacag
    11581 cgtgcttgct ctaatgcagc aacatgctca ggatcatgac cccaaagtac aacgtcatga
    11641 ccattgcgcg ctaaggtaat cgctaaagcg gtgccgtatg aaccggcacc gataactgtc
    11701 atagaagcgt tcattaagct tcttcagccc cttcattagc cgcatcagtt tgttgctgtt
    11761 gtaaatagtt cataaacaac gcatcaaagt taacaggtgc taagttcagt tgtgggaaag
    11821 taccacggct aactaagtta gtgatacatt cacgagcgta agggaacaga acgttaaggc
    11881 aatatgcacc taagcaatgt gcct
//
