LOCUS       KY710722               14544 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain CCUG 10704 O antigen gene cluster,
            complete sequence.
ACCESSION   KY710722
VERSION     KY710722.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14544)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14544)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14544
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="CCUG 10704"
                     /serotype="O54ab"
                     /culture_collection="CCUG:10704"
                     /db_xref="taxon:584"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     misc_feature    1..14544
                     /note="O antigen gene cluster"
     gene            2..1279
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1619..2014
                     /note="glycerol-3-phosphate cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wffW"
                     /product="wffW"
                     /protein_id="AXY99869.1"
                     /translation="MKRIITFGTFDVFHVGHVNILERAAALGNYLIVGISSDKLNFSK
                     KQRYPIYSQQDRTRIIKSLKFVDEVFLEESLELKLEYIKKYKADILVMGDDWTGRFDW
                     VKSHCDVIYLPRTPSISTTEIIEVVKNHK"
     CDS             2028..3173
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="CDP-glycerol:poly(glycerophosphate)"
                     /protein_id="AXY99870.1"
                     /translation="MKFIHKIILLILSIIYIFIPKDNKKIIFTSYKNMKYNFCSRIFF
                     EFLLQQENEYDIKFVINDNELRDYLNKNIGNYFITSYKFKDVVECLSSRIWISSCKPI
                     YSSPLSLVGHININVWHGIPLKKVGINDKHQSKIKKLIYKYYYSWCFYSLFVVSSEEV
                     KKLIHSSFNVKNNKIFISGSLIGENFSKSIDLEQNNKLKNILIKSRKNKVLYAPTYRD
                     GGKTQFFPFNGLDKNEFEKFLKKNEITIYIRPHHLEKNYKKYIEWEGIEYLGSDIISE
                     ITYYLENFNFLITDYSSILFDYMLTNGNLILLPYDYDIYKKERGLNFSIDEINYGDIA
                     TTLEDFKNSLLQRNKKNTNKDKIIDRFHSIRKDQCTTLLKKIKIYDK"
     CDS             3163..4272
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99871.1"
                     /translation="MINKIINIANIDPSEKAGGLNLMVKNIVNAQIKEGIDAKLLYFN
                     IPNYNLENSNKNIKYKNLISFCKLVTKDTHFIFHSIYNIKYIFLILIIILLGGNYSIH
                     SHGSLSKHSIKKSWVKRYCYIIAVKFMLIFSKKIIFSNHSEFLNSIIKNEKKIFFIPN
                     LIPATSNIQLEEQKDIKKIIYIGKIDYYYKGIDNLIIALSEFLDKNEDYTVDIYGFGN
                     NKNLDINNIDHKEKDIKKLLLDIKKYNLENKVNFLGPITGIKKIIALKKAGVFILTSN
                     SEAMPLSISEALCNSIPVIATKQTNMSEYIIPYNAGVICENNSVDILNALDTYHNNIV
                     CNYNYYSQNAFNCYQSELNSIYLNAYISRYIKELK"
     CDS             4269..5510
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99872.1"
                     /translation="MKKILQKYSDSIIATLALIINRGLDAIILLVITPFLIKIFGVDN
                     YGELSYYLAIIFFIQTFIIFGFENYIIYNTSNNKKNHSIILSSVMTIKITIFFILALI
                     YIIFCVISYTGDSIFPYILLLLPFSECFNFSHYYVVKKIPTKLIKISIIRLLFYVILT
                     LTLISNIKDIELYSLILSTSYLITVIIQNIYLKKEYNIKPHLVTNKKKYFLCFKDSFY
                     FFLLRLFQILSDRLYLILSGIALSYIFVTYLDVAIKLYLVILMPVQLLVTSVLPKFIT
                     KNYNFSFKVYTIFILLLSLILIPLSFHFEKSINNYFFHTKDIGLNIYIYVCISAIVFI
                     SSFLISELYLNPIGKLKLSLIISFISFIIPFSISLILSLSGALSFELILTAFLASKML
                     DFILKLSTYLYLKNMVRKKYV"
     CDS             5503..6870
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99873.1"
                     /translation="MYKNPNARNILLIFQFLLSLFFLSVFLIEKNNNSFKFDSNLPLI
                     VLQINFIYIVITSLFFIKKTSLYSIFIFCYFIFSLGRIFISNENLNSFSSIIGTTYFT
                     NEVINESILFNICIISLIHLGFLSSLYIYKVDNSPISPNPTLFNYARFLFFISLPLCL
                     VKFFFEIKTISDIGYVGYYKHGTDLPFIINIMRFIFEISFLIILASMPSYITFKRYTI
                     LFLLTMSLFLIIGVRSKFVLYFIFIFWYYYKFYAKKDISFLKIFFFLSISIILLILVQ
                     LNRQNWVFNSDLNYFKYFFISQGVSFYILPLTITYIEEFTNLIPSIFSSFSINSIIYT
                     SQTIERLNNVGFLGDIISYNHLRDYYLNGNGLGGSFIAELYQAGIIISIIFSILLGFF
                     INYFDTHVSQNRYFLAISFYVVSNIAYMPRSVFFKSPYMLISYFIIFLFLLSLPKITI
                     FTRKN"
     CDS             6880..7686
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99874.1"
                     /translation="MYFSVLMSVYKNEKKAHLDECLLSLSMQLAPPTEIILIIDGPIS
                     LELEKTIHNWDYKLNLVIVKLEKNMGLGHALNKGLEYCKYNFIFRMDTDDICDPSRFS
                     KQIEFLKNNPNIDLLGSATEEFDENMENSFGYRINPTEHNAIVNYAKKRNPFNHMTVA
                     FKKDAVLSVGGYQHHLFMEDYNLWIRMISSGYKVANLPDVLVKVRAGNSMVTRRKGIK
                     YIKSEYKLAKLKIKTHLDNSFSAYSIFLLRSIPRILPTSFLSKIYKILRK"
     CDS             7700..8647
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXY99875.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSDKYPEKWVFG
                     DVTKPESLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWYKKDNTNTLVVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLRFATQIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHSHIKVPYAIGLLGGYCFDILAKVTGKEFPVSSIR
                     IKKFCARTQFKSNNIANTHFKAPVSLEQGIANTVKNEFM"
     CDS             8881..10047
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99876.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKAFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             10060..10563
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99877.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             10670..11653
                     /note="orf11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="hypothetical protein"
                     /protein_id="AXY99878.1"
                     /translation="MHNVTLSFKYSHHISQYIYGFLLLSKNNIIKLSNIDKDTNITGA
                     QHILRANIDGKKIIYDANDGDHIDRGFFSIPDYEWCDLYFKRSYSNQLAEQFPKCRPL
                     GFNYEIKPFYGMIDRFFGNIRRLMGKEIVKHTDLEIIPNVSNNPKILFLTRLWEPNPL
                     KDNASKIELEYYNETIQLNKVRMDALNMIHTQFNERSTIGINDIPYSRRMAPDFILPK
                     EKTHRRNFINMMKEHEICITSTGLHKSTGWRFGEFVAASRAIISEPLNYVVPGNFDNY
                     LPFNNIEELYLAIEKLVNDKELRYDMMKRNNIYYNNYLKPDRLILNTITSL"
     CDS             complement(11711..12265)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXY99879.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEIKIGDNAIIGAH
                     ALVLKDVGERCRYITKVIADIRRV"
     CDS             complement(12268..13101)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99880.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFEYGDG
                     I"
     CDS             complement(13201..14214)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99881.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(14214..14504)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5017 a   2391 c   2276 g   4860 t
ORIGIN
        1 ccatgaaagt gaataccgtc aaggtgttat gctagagcaa catatagaag ccgaactagc
       61 gcaagatccg gctaacgatc tgctatggtg gcggcgatta atccgcgcca ttgataaatg
      121 ggcgccacct ggtcaacgtc ttattattgt caccagtgaa ggtcgtatta ttggtgctca
      181 acgtaatgag atacaagttg tcagaaactt tatgggacag tctgataacg cagaccatcc
      241 gaaaaagaaa aaatacggtc gctctgagat gttaggcccc ttttccatta gagatggtga
      301 agatcactat caactttact tagtgcgccc atcaagtagc ccacaatctg actttatcaa
      361 tttattattt gataaaccgc ttttattact gatattcacc atgcttatca gcacaccgct
      421 actggtttgg ctctcttgga gcttggcaaa accggcccga aaactcaaaa atgcggcgga
      481 tgatgtggca aaaggtaatc tgcgccctca ccctgagctg gaaaccggtc cacaagaatt
      541 tttagcggca ggcaccagtt ttaatcagat gatcagcgcc ttagaacgca tggttgaagc
      601 gcaacagcga ttgatttctg atatctccca tgaattgcgc accccgttaa ctcgtttaca
      661 gcttgccagt gcattactac gtcgtcgtag cggtgaaagt aaagagctag agcgtattga
      721 aacagaaaca cagcggctag atggcatgat caatgactta ttagtgcttt ctcgcaacca
      781 gtataaaaat gagttattgc gtgaaaccgt aaaagcgaat gagctttgga acgatatttt
      841 agataatgcg aaatttgagg ctgaacagag caataaaacc ctaacagtaa ccgccccgcc
      901 aggcccatgg acgatttatt gcaatcctta ttcattagcg agtgcctttg aaaatatcgt
      961 tcgcaatgcc ttgcgttatt cacattctcg tattgaggtg gcctttactg aacaaaatca
     1021 gggtatcact attattgttg atgatgatgg cccaggggtt agcccagaag atcgcgagca
     1081 tattttccga cctttttatc gtacggacga agcacgtgat agagaatccg gcggtacagg
     1141 gctgggactg gcaatagttg aaaccgcaat tagtcaacat cgaggccatg taaaagctga
     1201 cgatagccca ttagggggat taagagtgga gatttggttg ccgagaggga gtgtggtaaa
     1261 taaatcacaa cacgcttaat tcagtaataa aattactata ttaaattatt ctgccatcta
     1321 ttgaaaaata ggtggcatat tcttttcatt cccatcttta tagatatttc agaatgatct
     1381 tgctaaatca gtcgatattc cgatacttat atatacctaa tcaatgttag aatacccatc
     1441 aattacttga cccgctggtt tgaagagtct aatctcaaag cggtttagta tactgttaaa
     1501 gtatgcttat ataaaaaaac tcttatgtta cagatagata agagattttc gagaaatttt
     1561 ggcgctctgg gggccgtaaa cccagtgcgg tagctatgac taataaagag aattcaaaat
     1621 gaagcgtatt attacgtttg gtacatttga tgtgtttcat gtaggtcatg taaatatttt
     1681 agaaagagca gctgcattag gtaactattt aattgttgga atatcatcag acaaattaaa
     1741 tttttctaaa aaacaaagat atccaattta ttcacaacaa gatagaacaa gaattattaa
     1801 gtctttaaag tttgtggatg aagtattttt agaagagtca ttagaattaa aacttgaata
     1861 tataaagaag tataaggctg atattctggt aatgggtgat gattggacgg gtcgatttga
     1921 ttgggtaaaa tctcactgtg atgttattta tctaccacga actccctcta tctcaaccac
     1981 agaaataata gaagttgtta aaaaccataa ataataaata atatcatatg aaatttatac
     2041 ataaaattat acttttaatt ctcagcatta tctatatatt tatccctaag gacaataaaa
     2101 aaataatatt cacttcttat aaaaatatga aatataattt ctgtagtaga attttttttg
     2161 agtttctatt acaacaagaa aatgaatatg atataaagtt cgttataaat gataacgaac
     2221 tacgtgatta tttaaataaa aatattggta actattttat aacatcttat aaatttaaag
     2281 atgtagtaga atgtctatca tccagaatat ggatatcatc ctgtaaacca atttattcat
     2341 caccattatc acttgttgga catataaata ttaatgtttg gcatgggatt ccactaaaga
     2401 aagttggaat aaatgataag caccaatcta aaataaaaaa attaatatat aaatattatt
     2461 attcatggtg tttttatagc ttatttgtag tttcatccga ggaagtaaaa aaattaatac
     2521 atagtagctt taatgtaaaa aataataaaa tttttatttc cggctcttta ataggtgaaa
     2581 actttagtaa atctatagat ttagaacaaa ataacaaatt aaaaaacatt ttaattaaat
     2641 caagaaaaaa taaagtgttg tatgctccaa cttatagaga tggtgggaag actcaatttt
     2701 ttccttttaa tggactagat aaaaatgaat ttgaaaaatt tttaaaaaaa aatgaaataa
     2761 ctatatatat aaggccccat catttagaga aaaattataa aaaatatata gaatgggaag
     2821 gtattgaata tttaggtagt gatattattt cagagataac atattatcta gaaaacttta
     2881 attttttaat tactgattat tcaagtatac tctttgacta tatgctgacc aatggaaatc
     2941 taatattact cccttatgat tatgatatat acaaaaaaga aagaggatta aacttttcaa
     3001 ttgatgaaat aaattatgga gatatagcaa ctactttaga agattttaaa aactctttat
     3061 tacaaagaaa caaaaaaaat acaaataaag ataaaataat tgatagattc cactctataa
     3121 gaaaagacca atgtacaaca ttattaaaaa agattaaaat atatgataaa taaaataata
     3181 aatatagcaa atatagaccc atcagagaaa gctggcggac taaatcttat ggttaaaaat
     3241 atagtaaacg cacaaattaa agagggcata gacgcaaaat tactttactt taatatacct
     3301 aattataatc tagaaaatag taataaaaat ataaaataca aaaaccttat ttcattttgt
     3361 aagcttgtca caaaagacac acactttata tttcattcaa tatacaatat aaaatatata
     3421 tttttaattt taatcattat attgttaggt ggtaattata gtatccattc tcatggctct
     3481 cttagcaaac atagcataaa aaaatcttgg gtgaagagat attgttatat tatagcagtc
     3541 aaatttatgc ttattttctc taaaaaaata atattctcca atcattctga attcttaaat
     3601 tctataataa aaaatgaaaa aaaaattttt tttataccaa acttaatacc tgctacatct
     3661 aatattcaac tggaagagca aaaagacata aagaaaataa tttacattgg gaaaatagac
     3721 tattattaca aaggaattga taatttaatt atagcgcttt ccgaattcct agataaaaat
     3781 gaggattata ctgtagatat atatggcttt ggaaataata aaaatttaga tattaataat
     3841 atagatcata aagaaaaaga tataaaaaaa ttactcttag atataaaaaa atataattta
     3901 gaaaataaag ttaatttttt agggcccatt acaggaataa aaaaaataat agccttaaaa
     3961 aaagctggag ttttcatttt aacatctaac tcagaggcta tgcctttatc tatatcagaa
     4021 gcactttgca atagtatacc agtaatagca acaaaacaaa ccaatatgtc tgaatatata
     4081 ataccataca atgcaggggt catatgtgaa aataattcgg tggatatttt aaatgcacta
     4141 gatacatatc acaacaatat tgtgtgtaat tataactatt attcacaaaa tgcgttcaat
     4201 tgttatcaat ccgaattaaa tagtatctat ttaaatgcat atatttcaag atacataaaa
     4261 gaattaaaat gaaaaaaata ctccaaaagt attctgattc aataatagca actcttgctt
     4321 taataataaa tagagggtta gatgctatta ttttattagt cataactcca ttcttaataa
     4381 aaattttcgg tgtggataat tatggagagt tatcttatta tctagcaata atatttttca
     4441 tccaaacatt tataatattt ggatttgaaa actatattat ttataacaca agtaataata
     4501 agaaaaatca ttctatcatt ttatcatctg tcatgactat aaagattacc atctttttta
     4561 ttttggctct gatatatatt attttttgtg taattagtta tactggtgat agcatatttc
     4621 catatatact attattactc cccttttctg aatgttttaa tttttctcac tattatgtag
     4681 taaaaaaaat tccaacaaag ttaataaaaa tatcaattat tagactcctt ttttatgtta
     4741 tattaacact cacgttgata agtaatataa aagatatcga attatattct ttaattttat
     4801 cgacgtcata cttgattacc gtcattatcc agaatattta cttaaaaaaa gaatataata
     4861 taaaaccaca tttagtaacg aataaaaaaa aatacttttt atgctttaaa gattcatttt
     4921 attttttttt gctccgttta tttcaaatat tatccgatag actatacctg atactatcag
     4981 gtatcgcttt gagttatatt tttgttactt atctcgatgt ggcgataaag ctatatttag
     5041 taatattaat gcccgttcag ctattagtaa caagtgtttt accaaaattt attacaaaga
     5101 attataactt tagctttaag gtttatacta tatttatact attattgtct ttaatactta
     5161 ttcctttatc ctttcacttt gaaaaaagca ttaataatta ttttttccac actaaagata
     5221 ttggtcttaa tatatatata tatgtatgta taagtgctat agttttcatc tcatcatttt
     5281 taatatctga attatattta aaccctattg gtaaattaaa attatcactt attatttctt
     5341 ttatttcttt tataattcct ttttcaatat cactaatact ttctctttca ggtgcactat
     5401 cttttgaact aatactcact gcttttttag cttcaaagat gttagatttt attttgaaac
     5461 tgagcacata tctttattta aaaaatatgg ttagaaaaaa atatgtataa aaacccaaat
     5521 gcaagaaata tccttttaat atttcaattt ttattatctc tattttttct ttctgttttc
     5581 cttattgaaa aaaacaataa ttcatttaaa tttgattcta atttaccatt gatagtttta
     5641 caaattaact ttatatatat tgtaataacc tctttatttt ttataaaaaa aacaagctta
     5701 tattctattt ttattttctg ctattttatt tttagtttag gtaggatttt tatttcgaat
     5761 gaaaatctaa attctttttc gtctattata ggaacaactt atttcacaaa tgaagttata
     5821 aatgaatcca tcctatttaa tatttgtatt atatcattaa ttcatttagg ttttttatcg
     5881 tctttatata tatataaagt tgacaattct ccaatatcac caaacccgac tctttttaat
     5941 tatgctagat ttttattttt tatttcacta cctttatgtt tagtaaaatt tttctttgaa
     6001 ataaaaacaa tttctgatat tggttacgtt ggatattata aacatggtac agatttgcct
     6061 tttattataa atattatgag atttatattt gaaatatcat ttttaataat attagcctca
     6121 atgcctagtt atataacctt taaaaggtat acaattctat tcttactaac tatgagctta
     6181 tttttaatta taggtgttag aagtaaattt gttttatatt ttatatttat tttttggtat
     6241 tactataaat tttatgcaaa aaaagatatc agctttttaa aaattttctt ttttttatca
     6301 ataagtatta ttttattaat tttagtacag ctaaatagac aaaattgggt ttttaactct
     6361 gatctaaatt attttaaata ttttttcatt tctcagggag taagctttta tatacttcca
     6421 ctaacaatta cctatataga agaatttacg aatcttattc caagtatctt ttcatctttt
     6481 tcaataaact ctatcattta tacttcccaa actattgaac gtttaaataa tgtaggattc
     6541 ttaggtgata tcatatcata caatcatctc agggattatt atttaaatgg taatggtctt
     6601 ggtggtagtt ttatagctga actttatcaa gcaggtatta ttatttctat tatattttca
     6661 atactgcttg gtttttttat aaactacttt gatactcatg tttcacaaaa tagatatttt
     6721 cttgcaatat cattctatgt ggtttctaat attgcttata tgcctagaag tgtattcttt
     6781 aagtcccctt atatgttaat ttcttatttt ataatatttt tatttttact ttcccttccc
     6841 aaaataacaa ttttcacccg taaaaattag gataatttta tgtatttctc tgtattaatg
     6901 tctgtataca aaaatgagaa aaaagcccat ttagatgaat gccttcttag tcttagtatg
     6961 caactagcgc ctcctacaga aattattctt attatagatg ggcctataag tcttgaacta
     7021 gaaaaaacca tacataattg ggattataaa cttaatttag ttatagttaa attagaaaaa
     7081 aatatggggc taggacatgc actcaacaaa ggattagaat attgtaagta taattttatt
     7141 tttagaatgg atacagatga tatttgtgat ccctctcgct ttagtaagca aatagagttt
     7201 ttaaaaaata atcctaatat tgacttgctt ggttctgcaa cagaagaatt tgatgaaaat
     7261 atggaaaact cttttggcta tcgaattaat ccaacagaac acaacgctat cgtcaattat
     7321 gcaaaaaaaa gaaacccctt taatcacatg actgttgctt ttaaaaaaga tgctgttttg
     7381 tctgttggag gatatcaaca tcatttattt atggaagatt ataacttatg gataagaatg
     7441 atttcttctg gatataaggt ggctaattta cctgatgttc ttgtaaaagt tagagctgga
     7501 aactctatgg ttacaagaag aaaaggtatt aaatatataa aaagtgaata taaattagct
     7561 aaattaaaaa taaaaacaca tctagataat tctttttctg catattctat ttttctatta
     7621 agaagcatcc ctaggattct acctacctct tttttatcta aaatttacaa aatattaaga
     7681 aaataattaa ggtatttcta tgctaacaat catcggtggc tcaggcttta ttggcactcg
     7741 cttatcagat caattaacta aagaaaatat tgaatttaaa attgtggata ttgctaaaag
     7801 tgataaatat cctgaaaaat gggtatttgg cgatgtcact aagccagaat ctttactcga
     7861 accgttaaaa ggctctgatg taattatcaa tttagcagcg caacataaag ataatgttca
     7921 tcctattagc ttatattatg aagttaatgt cgatggtgct aaaaatgttt gtgatgttgc
     7981 tgaacaatta aatattaaac atattgtatt tacttcatct gttgctgtat atggttttgt
     8041 cgaaaaagaa acaggtgaag atggcgagtt ccatccattt aatgattatg gaaaatcgaa
     8101 acttgaagct gaacataagt atgaagcttg gtataaaaaa gataatacaa atacattagt
     8161 tgtaatcaga ccaaccgttg tatttggtga aggtaataga ggaaatgtat acaacctatt
     8221 ccgccaaata gcatcaggta aatttttaat ggtaggttca ggtaataata aaaaatctat
     8281 ggcttatgta gaaaatattg cggctttctt aagatttgct actcaaatag agtccggtca
     8341 tcatatattt aattatatag ataagcctga ttttactatg aatgagttaa ccgatattat
     8401 ctatactgct ctcgacaaac gccatagtca tattaaagta ccttatgcta ttgggcttct
     8461 tggtgggtat tgttttgata ttctggctaa agttacaggt aaagagtttc ctgtaagtag
     8521 tattcgaatt aaaaaattct gtgcacgtac tcagtttaaa tcaaataata ttgctaacac
     8581 tcattttaaa gcgccagtct ctcttgaaca aggtattgct aatacagtta aaaatgaatt
     8641 tatgtaataa ataaattcca caatatcttt ttattaacaa ataattaaaa tacactctac
     8701 acttctcgac ttaattttct aatcaaaaat taattttatg ttaattgtat aagtatatga
     8761 taaatattgt cgctcaatac actatacctt tactaaaaat tgaagaaaaa cactaaaata
     8821 tatttttatt ctagtatagt gaatttatat aaatacatat actttagata agagaaacac
     8881 atgaaaatca ccatttcagg aaccggctat gtcggtttat ctaacgggat actcctttcc
     8941 cagcacaaca aagttgttgc attagatatc aataaagaaa aagtcgaact attaaaccaa
     9001 aaaatctctc ctatctctga taaagaaatt gaagagtttt tagctactaa agagctcgat
     9061 tttacggcaa ctttagataa agcattcgct tataaagatg ctgaatttgt tatcattgcc
     9121 actcctaccg attatgatcc taaaaccaac tactttaaca cctcttctgt agagtcggtt
     9181 attcgtgatg ttttagaata taatccaaac accactatga ttataaaatc aaccattcca
     9241 gttggtttta ctaaacagat gagagaaaaa tatcataccg ataatattat cttctctcct
     9301 gagttcttac gtgaaggtcg cgccctttac gataaccttt atccatcacg tattgtgatt
     9361 ggtgagcaat ctgcaagagc agagaaattt gctgaacttt tacaacaagg ggcattaaaa
     9421 aaagatattg atgttctttt tactgactct acagaagcag aagcaatcaa attatttgcc
     9481 aatacttatc tcgcactgcg tgttgcttat tttaatgaac tcgatagcta cgcccaagct
     9541 tacggtctaa acgcaagaca aattattgaa ggtgtttgtt tagatccgcg tattggtaat
     9601 cactataata acccatcctt tggttatggc ggttactgct taccaaaaga tactaaacaa
     9661 ctgttagcca actacgaatc agtgcctaat aatattattg gcgccattgt ggaagctaac
     9721 cgcacccgta aagactttat tgctgattct attatcgcta aatcaccgaa gattgttggc
     9781 gtttatcgtt taattatgaa atcaggctct gataatttcc gctcatcttc aatccaaggc
     9841 attatgaagc gtattaaagc aaaaggcatt gaagtggtaa tttatgagcc agaaatgaaa
     9901 gaagagactt tctttaactc aaaactcatt cgtgatttag atgcgtttaa agccatgtct
     9961 gatgtcatta ttaccaatag aatggcttct gcactaaacg atgtggaaga aaaagtttat
    10021 actcgtgatc tctttggtaa cgattaaaac taattttata tgctcaatat cgtcttattt
    10081 gaacccgaaa ttccaccgaa tacgggtaat attatccgcc tgtgtgctaa cacaggcttt
    10141 catcttcatt tgatccaacc attgggtttt acttgggatg ataaacggct acgccgtgca
    10201 ggacttgatt atcgtgagtt tgctgatatc aaacagcacc acgattatta tgctttttta
    10261 gaaagtgaag gattaagccc tgataatgca caatcaccga caggtgcgcg attgtttgca
    10321 ttaaccacta aaggtacccc cgcccatagt aatgtaagtt atcaagatgg ggattatctg
    10381 ctatttggcc ctgagacacg aggtttacca ccttatgtgc tggataatat gccaacacaa
    10441 caaaaaatcc gtgtaccaat gttagcggat agtcgcagta tgaatctttc gaatacagtt
    10501 gccgtggttg tttttgaagc atggcgccaa ttaggctatc cgggtgcgtt attaagagat
    10561 tagttttact aattattgct tatattattt caaaaacgct ttgcattctt gataaagcgt
    10621 ttttatttat catcattttg cggtttaccg cttaaaatta ggttataaca tgcataatgt
    10681 tacattatct tttaaatatt ctcatcatat aagccagtat atatatggtt ttttactttt
    10741 aagtaaaaac aatataataa aactatctaa tatagataaa gatacaaata tcactggagc
    10801 gcagcatata ttaagagcaa atattgatgg taaaaaaata atatatgatg caaatgatgg
    10861 cgatcatata gatagaggtt ttttttctat tcctgattat gaatggtgtg acttatattt
    10921 taaaagaagt tattccaatc aattagcgga acaatttcct aaatgtagac ctcttgggtt
    10981 taattatgaa ataaagcctt tttatggaat gatagatcgt ttttttggta atattcgtag
    11041 gttaatgggt aaagaaattg taaaacatac tgatttagaa atcataccta acgtatcaaa
    11101 taatcctaaa atattatttc taactaggtt gtgggagcct aacccactta aagacaatgc
    11161 gtcaaaaatt gagttagaat actacaatga aacaatacaa cttaataaag tcagaatgga
    11221 tgcattgaat atgatccata cccaattcaa tgaaagatct actattggta ttaatgatat
    11281 tccatattca agaagaatgg cgccagattt tatattaccg aaagaaaaaa cacatcgaag
    11341 aaattttatt aatatgatga aagaacatga aatatgtatt acatcaactg ggttacataa
    11401 atcaacagga tggcgctttg gagaatttgt agctgcatct cgagctatta tttctgaacc
    11461 attaaattac gtagttcctg gtaattttga taattacctt cctttcaata atattgaaga
    11521 attatatctg gcaattgaaa agctggttaa tgataaagag ttaagatatg acatgatgaa
    11581 aagaaacaat atttattata ataattattt aaaaccagat aggttaatat taaatacaat
    11641 aacttcatta taattaacta tttttaatat acaattggct gataaaattt atcatatctt
    11701 atttaggact ttatacccgc ctgatatcgg caatcacttt cgtgatatac cgacaccttt
    11761 cccccacatc ctttaatact aatgcatggg cgccaataat cgcattatca ccaatcttaa
    11821 tctctccacc aataatggat gaattacagc caatataaac atggtttcct acttggatat
    11881 tggcttttcc ccctttctct tttacgccaa tagtcacccc ttgcataata gtgacatatt
    11941 cccccagttg acagtaatta gtcagtacaa tgccaatcgg gtgtggtaaa gaaaggcctt
    12001 taccaatacg agtttttaac gagatatcat tacagtattt acggttattg ctagcaccga
    12061 tccgaatacc taatttatgt aaaaagcgat ggggagagaa ataaaaatac tgtgctaccc
    12121 gccaccaaaa taggtaacgg gaacgctgac agaaaaatac tcgatgaaat agtcttaacc
    12181 aactaaaggg ttttttatca ccaatatatt cagcctgtaa gcaatctttt agttcagcaa
    12241 gggtaacctt agcttgagca tgcatagtta aataccatca ccatactcaa agccgggtac
    12301 aacaccatta aagtgttgat ccatatcgaa tgacggtttt tcactatcag gacgaccgac
    12361 aatgcgggca ggcacccccg ccaccgtagt atgagccggt acttcatgta atactaccga
    12421 gccagcacca attttagcac cacgaccaat ctcgatatta ccaagaattt tagctcctgc
    12481 gccaatcatt accccttcac gcactttcgg gtgacgatcg ccccctgttt tacctgttcc
    12541 ccctaaggtc acagattgta aaatggagac atcattttca accaccgccg tttcaccaat
    12601 tacaatacct gtcgcatggt cgagcataat accgtgtcca atacgtgccg ctgggtggat
    12661 atccacccca aaggtgactg atatttggtt ttgtagataa atggctagcg cttggcgatt
    12721 ttgttgccat agccaatggg caatacggta ggcttgtagt gcatgaaacc cttttaaata
    12781 gagcaatggg gtagaatatt tatccaccgc gggatcacgt aggcgtaccg cggagagatc
    12841 acaagcggcc gactcaatca ttgagctatc gtgactataa gcctcttcaa caatttcgcg
    12901 tactgcaatg gctggcatag tctgagttgc cagcttattg gccagcatat agcttaatgc
    12961 actacctaaa ttttcgtgtt tgagaagtgt cgcatgaaag aaactggcta acattggctc
    13021 acaatctgcc aatgcacgcg cttcgctttt aatatgatcc cagactcttg ttagttcttc
    13081 tagcgacata ctcactctca ttttttacac gaaagcctaa gtaaatactt aggctttgaa
    13141 ttttcaaaca aatggatagt taaaacagat accctaagaa tattatttaa aataaactga
    13201 ctacaggctg ctgtctatct catctttggt tgctcgccct aataaagcca atgcagcctc
    13261 ttttacatct ttatgttgat ataaaatttg gtaaatctgt tcggtgattg gcatttctac
    13321 acccacttgt tcggctaatg cgcgaacttc tttggtgtta cgataacctt cgactacctg
    13381 gccaattttc tcttgggctg tatcaacatc taaaccttga cctagcatca taccaaagcg
    13441 acgattacgt gattggttgt cagtacaggt taaaactaaa tcacccaaac cagccatgcc
    13501 cataaaagtt gccgcatctg cacctaaggc tttacctaag cggctcattt cggccagacc
    13561 acgggtaatc agtgcagtac gcgcattagc gccaaatccc ataccatcag acatacccgc
    13621 accaatagca atcacgtttt taacagcgcc ccccagttgc acaccgataa aatcagggtt
    13681 tttatagact cggaagcttt taccacaatg aaatagctgt tgtaactgtt ctaaaaagag
    13741 attatccgtc gaggccactg caatcgcggt gggcaaaccc gccgcaagct ctttagcaaa
    13801 agtagggcca gataatactg cgagcgggat ttcattacct aatacttcac gggcaacatc
    13861 ttgtaataag cgaccagtat gtgcttcaag accttttgtc gcccaaacaa cacgtgcatc
    13921 ctgacgtaaa aagggtttga tttgttgcag tacttcacca aacacatggc ttgggatcac
    13981 cacaagaata ttacggctcg cttcaatcgc tttttgcaaa gaagcttcca tatataaact
    14041 atcaggaaag gaaacatcgg gcagaaaggc ttgattacag cgcgcttgtt ctaatgctgc
    14101 aacgtgcttg ggatcatgcc cccacagcac aacatcgtgc ccattacgcg ctaaggtaat
    14161 cgctaaagcg gtgccgtatg aaccggcacc gataactgtc atagaagcgt tcattaagct
    14221 tcttcaactc ctgagtttgc tgcatcctgt tgctgttgtt gctgtaagta gttcataaac
    14281 aacgcatcaa agttaacagg tgctaagttt aattgtggga aagtaccacg acccactaag
    14341 ttggtgatac attcacgtgc ataagggaat agtacgttcg ggcagtatgc acctaagcaa
    14401 tgtgctaatt gtgtaccttc gataccttca acagtaaaga tacctgcttg ttgtacttca
    14461 cacaggaaag cgggtggccc ctgaaagtga ataccgtcaa ggtgttatgc ttgtggtact
    14521 tcacacagga aagcggtggg gagg
//
