LOCUS       KY710726               15615 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2654 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710726
VERSION     KY710726.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2654"
                     /serotype="O58"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..816
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..15615
                     /note="O antigen gene cluster"
     CDS             1197..2090
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlD"
                     /product="rmlD"
                     /protein_id="AXY99937.1"
                     /translation="MKIILFGKNGQVGWELQRSLAPLGTVIALDSKSIEYKADFLFPS
                     ELEKTILSIKPDIIVNAAAYTNVDKAETDDINAFKINAESVEVIAKTAEKIGALLIHY
                     STDYVFDGTNDIPWLETDIPAPLNIYGKSKLAGELAIINNCKKYLIFRTSWVYASKGN
                     NFAKTMLKLGKSHDSLSIIDDQYGAPTSAELLADCTAHAIKSASDNESLYGLYHLTAK
                     GTTTWFEYASLIFKYAQEYSLPLAVKNLTPIPAKEYPLPAFRPHNSRLNTQKFETNFK
                     LELPHWKFGIYRMLNEILDNK"
     CDS             2117..2995
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99938.1"
                     /translation="MKKYKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGIHLQYKVQPSPDGLAQAFILGEDFIGS
                     DNCALVLGDNIFYGHDLPKQLEQAIQRDQGATVFAYHVNDPERYGVVEFDQNKVAISL
                     EEKPLKPKSNYAVTGLYFYDNDVINIAKKLQPSPRGELEITDINKAYLEQHKLSVAMM
                     GRGYAWLDTGTHKSLIDASNFIQTIEERQGLKVSCPEEIAYRKGFINKEQVRILAEPL
                     SKNDYGKYLLRMIQSD"
     CDS             2998..3540
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="rmlC"
                     /protein_id="AXY99939.1"
                     /translation="MNIIKTEIPDVLILEPKVFGDHRGFFYESFNQKIFEEVVGYPVS
                     FVQDNHSKSNKNILRGLHYQTKNTQGKLVRVISGEVFDVAIDIRKDSPTFGKWVGAIL
                     SAENKYQLWIPKGFAHGFYVISEEAEFVYKCTDYYNPSAESSIIWNDEDINITWPNIC
                     TPILSDKDKNGLTLKEAPHL"
     CDS             3573..4805
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99940.1"
                     /translation="MPYFKTLFKNIFGLGSINIINLIIPIIIIPILSRTISSNDYGYI
                     LYITTLQLFSAIFLDYSFNITSVKEYITSNNKVRRNVIFIETQLSRLLLMLIYSFIVL
                     FYTIMIGYNIYDIIYYIIPFSLAHILISPWLYQAKCQLSIMGTVLFFSRVVHLILILV
                     YKDNKNIDSIVLISQTYTYLLSGLILFYISRNNYILCFKSKRKTLVLLRVKEKLLNSL
                     PLFIADFSPNLYTNIPAILLIDFINKAIYFQYNIAIKVVGIGLMLQSIISRAFFPILC
                     KDKNINQKIVFCINILPTLLFITPTILFSNHLFFIITGQSSILFQEYMVILSIGMFFG
                     VLGSYLGQNFLLVKTDGRKYSKVIVISCVISGIIGLFIVPIYTDFGAIWLLTIGRFFF
                     FIGCFYCFLEYKRQQNYV"
     CDS             4798..5814
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="hypothetical protein"
                     /protein_id="AXY99941.1"
                     /translation="MYELYIINNGKFSQSLYPWESLDLSEVKKNLANLNISAKEISFA
                     DINNSGLDFYKNKIILFGGSQNKIEKSLIEDYAYWLTKHNANVIPSYECLRALENKGF
                     QALSSESLLGNKKLKYFYNDSDKIFYSDDYVYKLIDGAGSNGVFRCENKNELTKKIKN
                     LKFKNINLNTLIPYIKQVIKKLIKWRYIPKKEEYYKPKINLVMQRFIRNLNCDYKVLI
                     FGKRYFVLKRNIASGDFRASGSNLFEIIETVPNEVLDMAKNIFTKLNTPYCSLDISID
                     ADNVANLIEYQCTHFGPYTYLTAIKQYFINNNGDWVSVDWERKSLEWAYAEAIAEYLN
                     EKLL"
     CDS             5811..6890
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99942.1"
                     /translation="MIAHFVYNLLSYSGAAFQAHSLCKCLGTKNNIIFNIGSSSKINY
                     YNKDGIIIIDLPLNYLKRLFYITILFITLKIKVVHLHGWILTGLIPAIFLRKKIVLKT
                     TMFGDDDIASIIKRNKKNLYFLKYLSSVISISTPIKKSNDKCIKNLALKCQSIYLPNG
                     VVDDELILDNHEKKPIFCTVGVISDRKGTLDAIQYFINNYMTLPDSKLYIVGPTPEDD
                     DYAESNIIYYQSCQKYSEKYPDKIIITGKLSKDKVYDIYKKSIAHIFLSRKEGMPNVI
                     LESMYFNCVPLITEIEGVAYDIIDNNKDGFIFKNREFINISFEMIQHIAINKLPHKKI
                     MDKFLLKNLSIEHKKNYENIICKKI"
     CDS             6862..8067
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99943.1"
                     /translation="MKISSVKKYNTPNLVFILSLIVFFLDAIVNRFISIPIYVSTLPI
                     FTLTLLFLYKNIFLYVFILFSTLLLASSYFYSLTIEDIGYFFYFLFFIFSFFYLKKEL
                     LNNNIILSLPLFYFIFFLTCLFFWVYLINIDYQPHVADMSAKGANIEYYREYHSGLFR
                     APHFPSYLFYFVSLTIFYFRKKLGKYKTFIFILICIMAILISGSRTPIFSLIFASLIY
                     FSTSRLRNITLSFLIIILVGILFINIEFILELSKGTPFFQYLSFIYTLKNEPERLSRI
                     QLFFVFINSVKDFSIYELFFGRSFSEQFEVMQSYIGQRLWFHNDILAIFYSYGLVGIV
                     IFTTVIISIYIKLIRPSNNTTINIYFLSIIVFSFVNGILYHPSYIIITLLFWMLKNER
                     SSSCIHSVR"
     CDS             8030..8911
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99944.1"
                     /translation="MKDRPVVSILSANYNNGHYLNDFFHSIENQSYTNFEIVIIDDCS
                     TDNSDEIIRQWILKGNLSINYIKLPKNLGFANALNTGLAHCKGTYIARIDPDDVMHPS
                     RLEKQIKILTEKNEISLVGSNAYYFQESINNLVGHSNFRSEPKWIKNKYLSGEYGMMH
                     GTIMFKATLFKDIKYSQSEVPAEDYSLISRMIKSGNTPLNSIEKLTYVRIHDNSVSND
                     LPLTTIIKVYILRMEIFNIKTSTLAMILKFFHFKFYRNFLKSKHFYSKIFYLSLSVLC
                     SPIKAFKIIYNRFISNV"
     CDS             8904..9962
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99945.1"
                     /translation="MYKLSIVGTVGVPACYGGFETLVENLLVEKNNKEYLVYASSRSY
                     SKKINSYKNADIKYLPFKANGTQSIIYDVLSILHSAFILKPQNILILGVSGTLILPFV
                     RPFFKGKIITNIDGLEWKRDKWNRLVKKFLKFSEKLAVKYSDIVITDNQGISDYVFKE
                     YDTKSMTIAYGGNIPEYLDKIETKQKEYYLKICRIEPENNVEMVLKAFSIHTDSKLIL
                     VGNWNASNYGKSLRKNYISFDNIKMLDPIYDPQKLHILRSECAGYIHGHSAGGTNPSL
                     VEIMHYGKIIYAYDCIYNRYTTNNISRFFSSPESLISLLKKNSNNDDILNECHAIKNY
                     ARLNYSWEKIREKYLSCI"
     CDS             10022..11188
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99946.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLVRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             11214..12224
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99947.1"
                     /translation="MKYLVTGAAGFIGFHLIEKLIQQGETVVGIDNLNDYYDISLKES
                     RLNILNQLNNFSFSLIDLADREKMASLFETEKFDKVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINDDPIDIYNNGEMKRDF
                     TYVEDIVEGIARIADVIPTPQQDWKVSTGTPANSSAPYKVYNIGNGSPVNLMDYISAL
                     ETHLGKVADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             12228..12731
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99948.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12802..13359)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99949.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRIYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIKQSSM"
     CDS             complement(13359..14192)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99950.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14271..15287)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99951.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(15287..15598)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5371 a   2612 c   2500 g   5132 t
ORIGIN
        1 tcaaaaaatg cggcggatga tgtggctaaa ggtaacctac gccctcaccc tgaactagaa
       61 actggccctc aagagttttt agccgcaggc acaagtttta atcagatgat aagtgctctt
      121 gaacgtatgg tagaggcgca acaacgtctg atctctgata tttctcatga actacgtacc
      181 ccattaacac gcttacaatt agccagcgca ttgttgcgtc gtcgtagtgg agagagcaag
      241 gagctagagc gtattgaaac tgagactcaa cgccttgatg gtatgattaa tgacttacta
      301 gtactttccc gtaatcagta taaaaacgag ttattacgcg aaacagtaaa agccaacgaa
      361 ttgtgggatg acattttaga taacgctaaa tttgaggccg aacaaagtaa taagacactg
      421 caagtcacaa cacctccagg agcctggcct atttactgca atccatattc tctggcgagt
      481 gcatttgaga atatagtccg taatgctctg cgttattcaa attctcgcat tgaagtcgcg
      541 tttactgaac aaaaccaagg tatcaccatt attgttgatg atgatggccc tggcgttagc
      601 cctgaagata gagagcatat cttccgacca ttctatcgca ctgacgaagc tagagatcgt
      661 gaatccggtg gtactggctt aggattagcg attgttgaaa ctgccgtcag ccaacacaga
      721 ggccatgtta aagccgatga cagtccatta ggtgggttga gggttgagat ttggttgccg
      781 agagcgggtg caagtaacaa atcgccaacg gcttaataga gtaattaaac tgttgtatca
      841 aaatatcctg ccatctattg aagaataggt ggcatattct ttttgttcgc atttattgat
      901 gcaattcaga atgatctggc tgaatcgatc gctattccga tatttatata tctacaatca
      961 atgctaaaat acccagctat tacttgaccg tctggtttga agagtctact ctcagtgcgg
     1021 attttcgcat attgttaaag tatgcttata taataaaact cttatattac atatagataa
     1081 gggtttttcg agataaattt tggcgctctg ggggccgtaa acccagtgcg gtagctatgt
     1141 atattcaatg ctataaatat aatatattaa tattagggta attaaagaac taaataatga
     1201 aaattatctt atttggaaaa aacgggcaag taggttggga attacagcgc tctctagctc
     1261 cattaggaac tgttattgct ttagattcta aatcaataga atataaggct gattttttat
     1321 tcccttctga actagaaaaa accattttat ctattaagcc tgatattatt gtcaatgctg
     1381 ctgcttatac aaatgtagat aaagctgaaa cggacgacat taatgcattc aaaatcaatg
     1441 ctgaaagcgt tgaagtaatt gcaaaaacag ctgaaaaaat tggtgcttta ttaatacatt
     1501 attcaacaga ttatgttttt gatggaacca atgatattcc ttggttagaa acagatattc
     1561 cagcaccttt aaatatttat ggtaaaagta agctagctgg tgaactagct attattaata
     1621 actgtaaaaa atatctaata tttagaacta gttgggttta tgcaagtaaa ggtaataact
     1681 ttgcaaagac aatgctaaaa ctgggtaaaa gccatgattc gctatcaata attgatgatc
     1741 aatatggagc acctacgagt gctgagctat tggctgattg cacagctcat gcgattaaaa
     1801 gtgcatcaga taatgaatca ctttatggct tgtatcattt aactgcaaaa ggaacaacaa
     1861 catggtttga atatgcatct ttaatattta aatatgcaca agaatattca ttaccattag
     1921 ctgttaaaaa tctaactcct atccctgcca aagaatatcc tttacccgca tttagaccac
     1981 ataattctcg tttaaataca cagaaatttg aaacaaattt caaattagaa ttgcctcatt
     2041 ggaagtttgg catatataga atgctaaatg aaattttaga taacaaataa atatcaatac
     2101 ttatagttta cagaaaatga aaaaatataa aggtattatt cttgctggtg gctcaggaac
     2161 tcggctttat cctgtcacaa tggcagtaag taagcagttg ttaccaattt atgataaacc
     2221 aatgatctat tatcctctta gcacccttat gttagcagga ataagagata tattaattat
     2281 tagtacacct caagatacac ctcgatttca acagctatta ggtgacggta gccaatgggg
     2341 tatccatctt caatataaag ttcaacctag tcctgacggc ttagcgcaag catttattct
     2401 tggtgaagat tttattggtt cagataattg tgctcttgtt ttaggtgata atatttttta
     2461 tggtcatgac ctccctaaac aattagaaca agcaattcaa agagaccaag gtgcgacagt
     2521 ttttgcttat catgttaatg atcctgaacg ctatggagtt gtcgaattcg atcaaaataa
     2581 agtagctatt tcattagaag aaaaaccact aaaacctaaa agtaattacg cagtcactgg
     2641 tttatatttc tatgataatg atgtcattaa tattgctaag aaattacaac cttccccgcg
     2701 tggtgaattg gaaatcactg atataaataa agcatatcta gaacaacata agctctcagt
     2761 cgccatgatg ggtcgtggat atgcctggtt agatacagga acacataaaa gtcttattga
     2821 tgcaagcaac ttcattcaaa ctatagagga aagacaagga ttaaaagttt cttgtccaga
     2881 agaaattgct tatagaaaag gttttatcaa taaagaacaa gtaagaatac ttgctgagcc
     2941 tttatctaaa aatgattatg gcaaatacct attacgaatg attcagagtg attaattatg
     3001 aatattataa aaacagaaat tcctgatgta ttaattttag agcctaaagt atttggtgat
     3061 catagaggct ttttttatga aagctttaat caaaaaatct ttgaagaggt agttggttat
     3121 ccagtaagtt ttgttcaaga taatcattca aaatcaaata aaaatatatt aagaggccta
     3181 cattatcaaa caaaaaatac tcaagggaaa ctggtaagag ttatatctgg tgaagttttt
     3241 gatgttgcca ttgatataag aaaagattcg ccaactttcg gcaagtgggt tggggctata
     3301 ttatctgctg aaaataaata tcaattatgg atccctaaag gatttgctca tggcttctat
     3361 gtaatatcag aagaagctga gtttgtatat aaatgtacag attattataa tccttcggct
     3421 gaaagttcca ttatatggaa tgatgaagat attaatatta catggccaaa tatttgtacc
     3481 cctatcctat cagataaaga taaaaatggg ctaacattaa aagaagctcc acacttataa
     3541 acactgttaa taaatagcgg taatatattt gcatgccata ttttaaaact ctttttaaga
     3601 atatatttgg acttggttca ataaatataa tcaatttaat tatacccata attattatac
     3661 ccattctatc aagaacaata tcaagcaatg attatggata tattctatat ataaccacac
     3721 tgcaactttt ttcagctata tttttagatt atagttttaa tatcacatcc gtaaaagaat
     3781 atataactag caacaataag gttagaagaa atgttatttt tatagaaact caactttcta
     3841 gattactatt aatgttaata tatagcttta ttgtattatt ttatacaata atgattggtt
     3901 acaatattta tgatattatc tattatataa tccctttttc tcttgctcat atattaattt
     3961 caccttggtt ataccaagcg aaatgtcaat tatcaattat gggtactgta ttattttttt
     4021 ctagagtcgt acatctgata ttaatacttg tatataaaga taataaaaat atagattcta
     4081 tcgtattgat atctcaaact tatacttatt tattatctgg gttaatatta ttttacatat
     4141 ctagaaataa ttatatttta tgctttaaaa gtaagagaaa aacacttgtt ttactgaggg
     4201 taaaagaaaa attattaaac tccttaccac tgtttatagc tgatttttct cctaatctct
     4261 atactaatat accagcaata ttattaatag actttataaa taaagcaata tactttcaat
     4321 ataatatagc aattaaagtt gtcggtattg gattaatgtt acaatcgatt atatctagag
     4381 ctttttttcc tattctgtgt aaagataaaa acattaacca aaagattgtt ttttgtataa
     4441 atatacttcc tacattgctc ttcattacac ctactatatt attttctaat cacctttttt
     4501 tcattattac tggacaatct agtatattat tccaagaata tatggtgata ctctcaatag
     4561 gtatgttttt tggtgtacta ggtagctatc ttgggcaaaa ttttttattg gtcaaaaccg
     4621 atggaagaaa atattcaaaa gtaatagtca tttcctgcgt aatatcagga ataataggat
     4681 tgtttatagt gccaatttat actgattttg gtgctatatg gctattaacc atcggtagat
     4741 tcttcttctt tatcggatgc ttttattgtt tcttagagta taaaaggcaa caaaattatg
     4801 tatgaattat atatcattaa taatggaaaa tttagccaaa gtttatatcc atgggaaagt
     4861 ttagatcttt ctgaagtaaa aaaaaatctt gcaaatttaa atatttctgc taaagaaatt
     4921 tcattcgctg atataaataa ttcgggacta gatttttata aaaacaaaat cattttattt
     4981 ggtggtagtc aaaataaaat agaaaaatca cttattgaag attatgcata ctggctaact
     5041 aaacataatg caaatgtcat tcctagctac gaatgtttaa gggctttaga aaataaagga
     5101 tttcaagcat tatcatcaga atccttacta ggaaataaaa agcttaaata tttttataat
     5161 gattctgaca agatatttta ctcagatgat tatgtatata aacttattga tggcgctggt
     5221 agtaatggtg tatttaggtg tgaaaacaaa aatgaattaa caaaaaaaat taaaaattta
     5281 aaatttaaaa atattaattt aaatacttta ataccttata ttaaacaagt tataaaaaaa
     5341 ctgataaaat ggagatatat ccctaaaaaa gaagaatatt ataaacctaa aattaattta
     5401 gtaatgcaga gatttattag aaatttgaac tgtgattata aagttcttat atttgggaaa
     5461 agatattttg tattaaaaag aaatattgct tctggagatt tcagagctag tggtagtaat
     5521 ctatttgaaa taattgaaac agtacctaat gaagttttag acatggctaa aaatatattt
     5581 acaaaattaa atacaccata ctgttctcta gatatttcta ttgatgctga taatgttgct
     5641 aatcttattg aatatcaatg tacgcatttt ggaccatata catacctaac agcaatcaaa
     5701 caatatttta ttaacaataa tggagactgg gtttcagtgg actgggaaag aaaaagctta
     5761 gagtgggctt atgccgaagc tatcgccgaa tatttaaacg agaaattact atgatagcac
     5821 attttgttta taacttactt tcctatagtg gggctgcttt tcaagctcat tcattatgta
     5881 agtgcttagg tactaaaaat aatataatat tcaatattgg gagctcatca aaaataaatt
     5941 attacaataa agatggaatt attattattg atttacctct gaattattta aaacggttgt
     6001 tttatataac tatacttttt atcactttaa aaataaaagt tgtacatttg catggatgga
     6061 ttttaacagg gctaattcca gcaatatttt taagaaaaaa aattgtatta aaaaccacta
     6121 tgtttggtga tgatgatatt gcatctatca tcaaaagaaa taaaaaaaat ctatacttcc
     6181 taaaatatct atcatctgta atatcgatct caacccctat aaaaaaatct aatgataaat
     6241 gtattaaaaa tttagcatta aaatgccaat ctatatactt gcctaatggt gtcgtggatg
     6301 acgaattaat attagataat catgaaaaaa aacctatttt ttgtaccgtt ggtgtaatct
     6361 ctgataggaa aggcacctta gatgctatcc aatattttat taataattat atgacccttc
     6421 ctgattctaa attatacatt gtaggaccga cacctgaaga tgatgattat gctgaatcta
     6481 atattattta ttatcagagt tgtcaaaaat acagtgaaaa atatcctgat aaaatcatta
     6541 ttactggtaa acttagtaaa gataaagttt atgatatata taaaaaaagc attgctcata
     6601 tcttcttatc aagaaaagaa ggtatgccaa atgtaatttt agaatctatg tactttaatt
     6661 gtgtcccttt aattacagaa attgaaggtg ttgcatatga tataatagat aataataagg
     6721 atggttttat ctttaaaaat agagaattta ttaatatttc ttttgaaatg atccaacaca
     6781 ttgctataaa taaacttcct cataaaaaaa taatggataa atttttatta aaaaatttat
     6841 ccatagaaca taaaaaaaac tatgaaaata tcatctgtaa aaaaatataa cacacctaac
     6901 ttagtattta tattatcttt gattgtattt tttttagatg caatagtaaa tagatttatt
     6961 tctattccta tttatgtatc gacacttcca atttttactt taacactact atttttatat
     7021 aaaaatatat ttttatatgt ttttattcta ttttccacct tactattagc atctagttat
     7081 ttttatagtt taactataga agatattgga tatttttttt attttttatt ttttattttt
     7141 tctttttttt atttaaaaaa agaactttta aataataaca ttatattatc actaccatta
     7201 ttttatttta ttttctttct tacttgtctt tttttctggg tatatctaat aaatattgac
     7261 tatcaacctc atgttgcaga tatgtcagcc aaaggagcaa atattgaata ctacagagaa
     7321 tatcattcag gactatttag ggctcctcat tttccttcat atttatttta ttttgtctcc
     7381 ttaacaattt tttattttag aaaaaaatta gggaaatata aaacatttat ttttattctt
     7441 atttgcatta tggccatttt gatttcagga agtagaaccc ccattttctc tctaattttt
     7501 gcatctctga tttatttttc aacaagccgg ttacgtaata ttacgttaag ttttttaata
     7561 ataatactcg ttggtattct ttttattaat atagaattta tattagagtt atcaaaaggc
     7621 actccatttt ttcaatattt atcatttatt tatacattaa aaaatgaacc tgaacgttta
     7681 tctagaatac aactcttttt tgtatttatt aatagtgtaa aagatttctc aatttatgaa
     7741 ttgttttttg gtagatcctt tagtgagcaa ttcgaagtaa tgcaatctta tataggacaa
     7801 cgcctgtggt ttcacaatga tatacttgct attttttact cttatggatt agttggaata
     7861 gtaattttta ccacagtaat aatatctata tatataaaac ttataaggcc aagtaacaac
     7921 actactatta atatatattt cctatcaata atagtatttt cctttgtaaa tggtatttta
     7981 taccaccctt catatataat tattacactt ttattttgga tgcttaaaaa tgaaagatcg
     8041 tccagttgta tccattctgt ccgctaatta taacaacgga cattatttaa atgatttctt
     8101 tcatagtata gaaaatcaga gttacactaa ttttgaaatt gttattattg atgattgttc
     8161 cactgacaat tcagatgaaa taatcagaca atggatatta aaaggcaacc tatctattaa
     8221 ctatattaaa ttacctaaaa acttaggttt tgctaatgct ctaaatactg gattagctca
     8281 ctgtaaaggt acatatattg cacgaattga tcctgatgat gtcatgcatc catctagatt
     8341 agaaaaacaa attaaaattt taaccgaaaa aaatgaaatt tcattagttg gttctaatgc
     8401 ttactatttt caagaatcaa taaacaatct tgtaggccat tcaaattttc gttctgaacc
     8461 caaatggata aaaaataaat atctttcagg cgaatatgga atgatgcacg gaacaataat
     8521 gtttaaggct acattattta aagatattaa atattcacaa tctgaagttc ctgcagagga
     8581 ttattcactc atttctagaa tgataaaatc aggcaatacc ccattaaatt ctatagaaaa
     8641 attaacatat gttagaatcc acgataatag cgtatcaaat gatttaccat taactacaat
     8701 aataaaagtt tatattctca gaatggaaat atttaatata aaaacatcca ctctagcaat
     8761 gatattaaag ttttttcatt ttaaatttta tcgcaatttt cttaagagca agcattttta
     8821 tagtaaaata ttctaccttt ctctttctgt tttatgttct ccaataaagg catttaaaat
     8881 tatttataat aggtttatat caaatgtata aattatctat agtaggtaca gttggtgttc
     8941 ctgcatgcta tggtggtttt gaaaccctag tagaaaattt actagttgaa aaaaataaca
     9001 aggaatacct tgtttatgct tcttcgcgat cttattctaa aaagataaat agttataaaa
     9061 atgctgatat taaatatttg ccatttaaag ctaatggtac acaaagtatt atttatgatg
     9121 tcttatctat tctacactca gcatttatac taaaacctca aaatatactt attcttggtg
     9181 tttctggaac tttaatactt ccatttgtac gcccattttt taaaggtaaa ataattacaa
     9241 atatcgatgg actagaatgg aaacgagata agtggaatag acttgtaaaa aaattcttaa
     9301 agttctcaga aaaattagca gtaaaatact cagatatagt tataacagat aatcaaggca
     9361 tatctgatta tgttttcaaa gaatatgaca ctaaaagtat gactattgcc tatggtggga
     9421 atatacctga gtaccttgat aaaatagaaa ccaaacaaaa agaatattac ttaaaaatat
     9481 gtagaattga acctgaaaat aatgtcgaaa tggttttgaa agcattttca atacatacag
     9541 actctaaact cattcttgtt gggaattgga atgcaagtaa ttatggaaaa tccctaagaa
     9601 aaaattatat atcttttgat aacatcaaaa tgttagaccc tatttatgac ccccaaaaat
     9661 tacatatact acgaagtgaa tgcgctggtt atatacatgg acattcggca ggtggaacaa
     9721 atccatccct agttgaaatc atgcattatg gaaaaataat ttatgcatac gattgtattt
     9781 ataatcgata tacaacaaat aatatatctc gctttttttc aagtcctgaa tcattaatat
     9841 ctttattaaa aaaaaatagc aacaatgatg atattctaaa tgaatgccac gctataaaaa
     9901 attatgccag actaaactat tcttgggaaa aaatcagaga aaaataccta agttgtattt
     9961 aataaaggaa tttataaaat attattttta ataaacaatt tcttcttaaa aagaggctac
    10021 tttgaaaatc accatctccg gtaccggcta tgtcggtcta tctaacggga tactgctttc
    10081 ccaacacaac aaagttgttg cgttagatat caataaagaa aaagttgaat tacttaacaa
    10141 aaaaatctcg ccaattacag acaaagaaat tgaagagttt ttagcaacca aagagctcga
    10201 ctttacagca acattagata aagaattcgc ttataaagat gctgaattcg ttatcattgc
    10261 cacaccaacc gattatgatc ctaaaacgaa ctatttcaac acctcttctg tagagtctgt
    10321 tattcgtgat gttttagaat ataatccaaa cacgacaatg attataaaat caaccattcc
    10381 tgttggtttt accaaagaga tgagagagaa atataatacc aataatatta ttttctcccc
    10441 tgagttctta cgtgaaggtc gtgcgcttta cgataactta tatccatctc gtattgtgat
    10501 tggtgaacaa tcagaaagag cacaaaagtt tgctgacctt ttacaacaag gcgcactgaa
    10561 aaaagatatt gatgttctgt tcactgactc cacagaagcc gaagcgatta aattatttgc
    10621 taatacttat ctagcactcc gtgttgctta ctttaatgag ctagatagct atgccgaatc
    10681 ttatggccta aatgcaagac aaatcattga aggtgtctgc ttagatccgc gtattggcaa
    10741 ccactataac aatccatcat tcggttacgg cggttactgc ttaccaaaag ataccaagca
    10801 actgttagcc aactacgact ctgtccctaa taatattatt agtgcaattg tcgaagctaa
    10861 ccgcacacgt aaagatttta ttgcagactc tattatcgct aaatcaccca aaatcgtcgg
    10921 cgtatatcgt ttaattatga aatctggctc ggataatttc cgttcttctt ctatccaagg
    10981 cattatgaaa cgcattaaag ccaaagggat tgaagtggtg atttatgaac cagagatgaa
    11041 agaagaaacc ttctttaatt caaaattagt tcgtgattta aatgagttta aagcaatgtc
    11101 tgatgtcatt attaccaata gaatgtcatc agcactaaac gatgttgaag aaaaagttta
    11161 tactcgtgac ctttttggta acgattaatt aataaatacg ggttaattac gtaatgaaat
    11221 atttagtcac gggtgcagca gggttcattg gttttcatct aatagaaaaa ctaatccaac
    11281 aaggtgaaac tgttgttggt atcgataatc tcaatgatta ttatgatatt tccttaaaag
    11341 aatccagact taacattctg aatcaattaa ataacttttc attttctctt attgatctag
    11401 ctgataggga aaaaatggct agtctatttg aaactgagaa atttgataaa gtgattcatt
    11461 tagctgcgca agcaggcgtt agatattcac taattaaccc gttttcttat gctgatagca
    11521 atttaacagg ctttttaacg attttagaag ggtgtcgcca taataatgtc aagcatcttg
    11581 tttatgcttc atcaagttct gtttatggct taaatgatga attgccattt tcacctcacg
    11641 atcaagctaa tcatcctgtt tcattatatg cagcaactaa aaaagctaat gaattaatgg
    11701 ctcacagcta ttctcattta tatggtatac caacaactgg attacgcttt ttcactgtat
    11761 atggcccttg gggtcgccct gatatggcat tatttaaatt cacaaaagcc attatcaacg
    11821 atgatcctat cgatatttat aataatggtg aaatgaaacg cgactttact tatgttgaag
    11881 atatcgtgga aggtattgct cgtatcgctg acgtaatacc aacaccgcaa caagattgga
    11941 aagtcagtac aggaactcca gctaatagct ctgcacctta taaagtctat aatattggta
    12001 atggttctcc agttaattta atggattata ttagtgcatt agaaactcat ttaggaaaag
    12061 ttgcggataa aaatatgtta ccaatgcaac ctggggatgt ttacactacg tgggcagaca
    12121 ctgaagattt atttaaagca actggctata agcctcaaac aagtgtcgat gaaggtgtaa
    12181 aacagttcgt tgattggtat aagaactatt atcaagttaa ataaaccatg ctcaatatcg
    12241 tcttatttga acccgaaatt ccaccgaata cgggtaatat tatccgcctg tgtgccaaca
    12301 caggctttca tcttcatttg atccaaccat tgggttttac ttgggatgat aaacgtctac
    12361 gtcgtgcagg gcttgattat cgtgaatttg ctgatattaa acagcaccac gattattatg
    12421 cttttttaga aagtgaaggt ttaaaccctg ataatgcaca atcaccatca ggtgcgcgtg
    12481 tatttgcctt aaccacaaaa ggcacgccag cacatagcaa tgtcagttat caagatggag
    12541 attatctcct gtttggccct gaaacccgtg gtttgccacc ttatgtatta gataatatgc
    12601 caacacagca gaaaatcaga gtcccaatgc ttgctgatag ccgcagtatg aatctgtcaa
    12661 atacggttgc ggttgtggtt tttgaggcat ggcgccaact tggttatcct ggtgcattac
    12721 taagagactg aaatgcaaaa agcgctgatc ataaataatg actagcgctt tttaacttca
    12781 aataactaac atgttaattt attacattga ggattgtttt atttcagcaa caaccttcgt
    12841 gatatatcga cacccttccc ctacatcctt cagtactaat gcatgagctc caatcaccgc
    12901 attatcacca atctctatct caccgccgat aatcgatgag ttacaaccaa tataaacgtt
    12961 attaccaacc cgaatatccg ctttgccatt cttttctttt acaccgattg tcacaccttg
    13021 cattaacgtc acattctcgc ctagctgaca gtaattagtt agtacgatcc ctatgggatg
    13081 tggtaatgaa aggcctttac ctatgcgcgt tcttagtgag atatcgttgc aatatatacg
    13141 attgttttta gcaccaatac gaatgcctaa ttttttgata aaacgatttt tagaaaaata
    13201 aaagaattgg gcgacacgcc accaaaatag ataacgtgaa cgttggcaga aaaaaacacg
    13261 gtgataaagt cttatccaac taaaaggctt gttatcacct atatactcag cgtgtaaaca
    13321 agcctttagt tcagaaaggg tgacttcagt taatgccatt aaatgccatc accacattca
    13381 aaaccgggta cgacaccatt aaagtgttga tccatatcga gtgaaggctt gtcactggtc
    13441 ggtttaccca caatgcgagc aggaacacca gcaacggtag tgtgcgcagg aacttcatgt
    13501 aaaacgactg atcccgcacc aattttggca ccgcgaccaa tctcgatatt gccgagtatt
    13561 tttgcacccg cgccaatcat aacaccttca cgcactttag gatgacggtc accgcaagtt
    13621 ttacctgttc cacctaacgt cacagattgt aaaatagaaa catcgttctc tacgatagcg
    13681 gtttcaccaa tcacaatgcc tgttgcgtga tcaagcataa taccgtggcc aatacgggca
    13741 gctggatgaa tatcaacacc aaaggtcaca gagatctggt tttgcagata caccgctaac
    13801 gctttgcgat cttgattcca taaccaatgc gcgatgcgat aagcttgtaa ggcatggaaa
    13861 ccttttaaat aaagcagtgg tgtagagtat ttatcaactg ccggatcgcg taaacgcaca
    13921 gccgaaatat cataagctgc cgattcaatc atagaattat cactacgata tgcttcttca
    13981 acaatctccc gaacagcaat cgcgggcatt gtttgagttg ctagcttatt tgccaacata
    14041 taacttaagg cactacctaa gttttcatgt ttgagtaatg tcgcatggaa aaaactcgcc
    14101 aaaattggct cgcaatctgc caagcatctt gcttcatttt taatatgatc ccagactctt
    14161 tttagctctt ctagcgacat actcactctc attcttttta cattgaagcc taagtaaaga
    14221 cttaggcttc ttatgtttaa aactgaaata aataactatc taaaatagac ttagaacaga
    14281 ttgctgtcta tctcatcttt agtagctcgg cctaataaag ataatgccgc ttctttcgcg
    14341 tctttatgct gatataaaat ttggtagatc tgctcagtaa taggcatttc aacaccaact
    14401 tgttcggcta atgcgcgaac ttcttttgta ttacgatagc cttcaactac ttgtccaatt
    14461 ttttcttggg cagtctccac atcaaggcct tgacctaaca tcataccaaa gcgacgatta
    14521 cgagattggt tatcggtaca ggttaaaact aagtcaccca accccgccat gcccataaag
    14581 gttgcagcat ctgcacctaa cgctttacct aaacggctca tttcagcaag gccacgagta
    14641 atcaatgctg tacgcgcatt agcaccaaac cccataccat cagacatacc tgcgccaata
    14701 gcaattacgt tctttaccgc accacctaat tggacaccga taaagtcagg atttttatat
    14761 actcggaaac ttttgccaca atggaacaac tgttgtaact cttcagagaa ggtattgtcc
    14821 gttgaagcca cagaaatcgc agtaggcaga cctgctgcaa gctctttggc aaaagtaggg
    14881 ccagataaaa ctgcaagcgg gatttcatta cctaatactt cacgcgctac atcttgcaat
    14941 aagcgaccag tatgagcctc aagtcctttt gtcgcccaaa caacacgtgc atcctgacgt
    15001 aaaaagggtt tgatctgctg taatacatca ccaaaaacat ggcttgggat cacaaccaga
    15061 atattacggc tagcttcaat ggctttttgc aaagaagctt ccatgtataa actatcagga
    15121 aaggaaacat cgggcagaaa ggcctgatta cagcgagctt gctctaacgc agcaacatgt
    15181 tcaggatcat ggccccaaag tacaacatca tggccattgc gcgctaaggt aatcgctaaa
    15241 gcggtgccgt atgaaccggc accgataact gtcatagaag cgttcattaa gcttcttcag
    15301 ccccttcatt agcggcatca gtttgttgct gttgtaaata gttcataaac aacgcatcaa
    15361 agttaactgg tgctaagttc agttgtggga aagtaccacg gctgactaag ttagtgatac
    15421 attcacgagc ataagggaac agaacgttag ggcaatatgc acctaagcaa tgtgctaatt
    15481 gagtgccttc aataccttca acggtgaaga tacctgcttg ttgtacttca cacaggaaag
    15541 cggattgtcg ttcttctccc cctgcgggtg gcccatgaaa gtgaataccg tcaaggtgtt
    15601 atgcttgttg tactc
//
