LOCUS       KY710739               12527 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Cosenzaea myxofaciens ATCC 19692 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710739
VERSION     KY710739.1
KEYWORDS    .
SOURCE      Proteus myxofaciens ATCC 19692
  ORGANISM  Proteus myxofaciens ATCC 19692
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12527)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Proteus myxofaciens ATCC 19692"
                     /mol_type="genomic DNA"
                     /strain="ATCC 19692"
                     /culture_collection="ATCC:19692"
                     /type_material="type strain of Proteus myxofaciens"
                     /db_xref="taxon:1354337"
                     /collected_by="American Type Culture Collection, USA"
                     /note="serogroup: O60"
     misc_feature    1..12527
                     /note="O antigen gene cluster"
     gene            2..643
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1060..2148
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="hypothetical protein"
                     /protein_id="AXZ00128.1"
                     /translation="MCNLIAIFGTSTKKNETRIPLHPDLFNLIPQKIKKNLLFEKGYG
                     KRFNISDEYFSEFFAGIASREDLFSLSDIWILPKPEKEDFKFFSKNKILFGWLHCVQG
                     REITQAATENGMTLIAWEAMYGGNDKTHIFNRNNELAGYAAVQHMLMLQGINGYFGKK
                     LKAAVLGFGATARGAINSLKSLGINEITTFSNRPQFLIKSPIESIDYKRIITENKVVY
                     LESNNQEKKESIDVLKNYDIIINCVLQNPTSPIIFIKNNDIDKITKKTTIIDISCDKN
                     MGFEFASPTYFDEPFINISENIKYYSIDNTPSIYWDSASYEITKSLIPFLELFISNLW
                     KKDPIIKNAIEIENGIIKNKKIIEFQKR"
     CDS             2216..3130
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00129.1"
                     /translation="MKNLIKKTLKIILTQITKISPKLSSKLHYYYVFHKKLNIKKPST
                     FNEKIQWLKLNIYPRDKLYTICADKYKVRDYVVNKDCREILNELIASYDSINEIDWDT
                     LPNGFVIKCNHGAGYNIICQNKKNLDINMTKELIKRWLNEDYWLKMAETHYKKIPKKI
                     IIEKLIKNEKNQLPEDYKVYCFNGKPEFVMICCERNTGHPKYYFVDKNFNLLPFGSAY
                     EKNPNIKLDKPLGFDELFIYAKKLCQDFVFVRADFYINDGNIIFGELTFTPAAGLDNK
                     ISNVLLPNGDAIMGEYLKLPNLPYHNKG"
     CDS             3142..4461
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00130.1"
                     /translation="MYRNFVEKLSYFFSNNLSIIKNSSWIIIEKLLSIFGLIFITSYV
                     AKYIGPDNFGKLSYAISIFIIIQTVSMLGTDTILFKRISKNRDSGISLMLSTIELRIL
                     LYILISILFTLYSIYTTKDHITIIFTISVAFSYLFLAIDHYAIYFNAILQSKINVLFN
                     IIGISISLLLRYLIVLFELNIIYFSIPIILTTLLPFIFRCIYFHRKIALKKIKKRKPY
                     IIYLMRQGLPISISNVSISVYSKISQIFLSLLISNYALGIYTVASTLAYAWLFLPQAV
                     ITSYFTKIYSKNNKNEILQLTAYLNLFIIFISVLYLIFIYFFGEKIILILYGIDYIES
                     ANIIFYLSIASVFSSLGIVSYRYIVAFSGYSFLSKKMFFTSFLGILLTYVLIKEYAIT
                     GAVLASILIEIVSLTVFNYFFKNKLVFFMHIKTIYIIFNFSKKHIKE"
     CDS             4469..5392
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00131.1"
                     /translation="MNKLIESHRTPLVSVIIPTYGRPHFLEKALDSILSQSYKNIEII
                     VVDDNPNNSNHKKTKEIINKYKYKCKIIYIFDGINVGGSLARNKGIIESKGDYITFLD
                     DDDIYLKYKVEKQVEHIINNNLDVSVCNMFFKKNDKFLEISNCYSRVRSIQEFILNGN
                     AYTPMIMSKRKSLLDVGLFTHSPRYQDHILMLKLFMADKKIKSLNEKLFIHNDHDEQR
                     ITYSNKSEEAYKIRQLYEKKNLYRLDVKEKKQYKIIELLITAKILRAKKNYFNSLKNI
                     FFSFMYITNKNDLYKIIKTLIRIHIFPNKNI"
     CDS             5394..6500
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00132.1"
                     /translation="MNMNTLKYNPFVTAFFLLYTLYSIKSGFFSLAIKNEFIFQIKDI
                     VNISIISIAIFFSFLLSKNKTKIYLIIFNILSIVGLLYFHNKFYLGFLLTISMLTLTS
                     KIKWEVIIKIIIFSNAITILFLCFSVYFSEYYFLEDDRFGRRFTAGFDNPNTLGQYIL
                     MLFTVISLFLELKVKNPSTRLIITTSIFIIIFTILYLTYSRTSLILSSLFYIIIIYSI
                     LLKKNNHFLPRIKLKYLILFSSLFFIFLQFYFIINFKENNFLYTVNDILTSRLWFGNI
                     LYSSLGFPNLLNGINIEKYSPIDFYFIQTIYSIGLIPFIFLYFLVIKNLFNTNITSLM
                     GYTLLIMLLETMTETYFSVPFYSIALFIIFSKKI"
     CDS             6502..7572
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00133.1"
                     /translation="MKTLLFCITDITTGGGTERALITLANKLANSNFDIHIYSLYKNN
                     PEPLFIINKNVKIYFLENKTHIFKLIKIAKIAKKHTSAIKIIISMGRLSIETSLLFKL
                     FNIKNLFLSEHTSFESYSPIIRSLKLLSYRFADKIIVLTEHDKLLLNNKYHLDKIYTI
                     PNINPYEEYSYNLNYNDKENIAIAIGRLCYAKNFERLINLWSKAITKNWKLLIIGDGN
                     DKKKLEKTISKSGVKNIEIIPFTKNITSYYKKSKLYLMTSRYEGLPMVLIESQHFGVP
                     IISFNCKTGPSEIIKNNYNGFLVDYYNDSLFIDKLNYFIKNESVQRKLSINSPIVSND
                     YSSKIIINLWENILSKNKSYEK"
     CDS             7562..8383
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00134.1"
                     /translation="MKNKTICVVIPIYINTNIHLFKKAIESILQQSHPADEILIICDG
                     LVTTQLNNLIKIFSNKYSYIKIIRYDYNKGPGYARDYGIKCTSCDYIAIMDSDDVSTI
                     DRLAIQYEFMQKNNDISLCGGLIRELDDKNHKTSIRNVPTSHSEILKTIKIKSPINNV
                     TAFFRRKDYLETDGYAHIRSSEDYALWARLIYKEKKLQNLPHIFVDVNFDIDSLSRRT
                     GLTHFKNDLFTQKELLNFNLITRYQFYKNILKYFIFRCVLHKNIKKILYKYYLRK"
     CDS             8391..9560
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00135.1"
                     /translation="MYKVTISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKIS
                     PISDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVI
                     RDVLEYNPNTTMIIKSTIPVGFTQEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIV
                     IGEQSARAQKFADLLQEGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSY
                     AQAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDAVPNNIISA
                     IVEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVV
                     IYEPEMKEDTFFNSRLIRNLDEFKTMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             9572..10075
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00136.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLKD"
     CDS             complement(10257..11078)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00137.1"
                     /translation="MSLEELKRVWDHIKSEARALADCEPMLASFFHATLLKHENLGSA
                     LSFMLANKLATQTMPAIAVREIVEEAYRSDVSMIESAAYDISAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWAQERQALAIYLQNQISVTFGVDIHPAACIGHGIMLDHAT
                     GIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVRQGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHAVPAHTTVAGVPARIVGKPSSDMPSFEMDQHFNGVVPGFECGDGI"
     CDS             complement(11140..12159)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00138.1"
                     /translation="MNNASMTVLGAGSYGTALAITLARNGHNVVLWGHDPAHVAALEQ
                     ARCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVVPSHVFGDVLQQIKPFLRQDSR
                     IVWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNG
                     YCEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALIT
                     RGLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGIDVDTAQD
                     KIGQVVEGYRNTKEVRALAEKVGVEMPITEQIYQILYQHKDVKEAASALLGRATKDGI
                     DSTLP"
     gene            complement(12159..12347)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4602 a   1929 c   1815 g   4181 t
ORIGIN
        1 acgaacacca ttaacacgct tacaattagc tagcgcatta ttacgccgtc gtcatggtga
       61 aagcaaagag cttgagcgta ttgaaaccga gactcaacgt cttgatggca tgattaatga
      121 tttgctggtg ctctctcgta atcagtacaa gaacgagcta ttacgtgaaa gcgtaaaagc
      181 taacgagctt tgggatgata ttttagataa tgctaagttt gaagcagaac aaagcaataa
      241 aacgttagca gtcaccaccc cacccggtgc ttggccaata tattgcaacc cttattcact
      301 tgcgagcgca tttgagaata ttgtccgtaa tgcactgcgt tactctaact ctcgcattga
      361 agtcgcattt acggaacaaa atcaaggtat cacaattatt gttgatgatg atggcccagg
      421 tgttagccct gaagatagag aacatatctt tcgccccttc tatcgcactg atgaagcaag
      481 agacagagaa tcaggtggta cgggattagg ccttgcgata gtggaaactg cggtaagcca
      541 acacagaggc catgtaaaag cagatgatag tccattaggc gggttaagag tagagatttg
      601 gttaccaaga gcaggtgcaa ctaataaaac ggttacatca taattgagta atataattac
      661 tgtatcaaaa tatcatgcca cttattagaa aataggtggc atattctttt tatgagccat
      721 atacagcata attcagaatg atcttgttag ttcaaccgtt attccagtat ttatatatct
      781 ataatcaatg ctagaatagc caccaattac ttgacccact gatttgaaga gtctactctc
      841 agtgcggttt acagcatatt gttaaagtat gcttatatga taaaactctt gaataacagc
      901 aagataagag ttttttaaga taaattttgg cgctctgggg gccgtaaacc cagtgcggta
      961 gcttttccat tgcttactag cagtgaaata aattcgataa gagaagtaat taaaagataa
     1021 tttaaaattt atttagaaat aaaactaata gagaaattta tgtgtaactt aattgcgata
     1081 ttcggcacat caacaaagaa aaatgaaaca agaatcccat tacatccaga tctatttaac
     1141 ttaatacctc aaaagattaa aaaaaatctc ctttttgaaa aaggctatgg aaaaagattc
     1201 aatataagtg atgaatattt tagtgaattc tttgccggaa ttgcatctag agaagattta
     1261 ttttcattat cagatatttg gatcttaccg aagccagaaa aagaggattt caaatttttc
     1321 tctaaaaata aaatattatt tggctggcta cattgcgttc aaggtcgtga gataactcag
     1381 gcagcgactg aaaatggtat gacattgatc gcatgggaag ctatgtatgg tggcaatgat
     1441 aaaacacata ttttcaatag aaataatgaa ttagcaggat atgccgcagt ccaacacatg
     1501 ctaatgttac aaggcattaa tggctatttt ggtaaaaaat taaaagcagc tgtactaggg
     1561 tttggagcta ctgcaagggg agctataaat agcctaaaat cattaggaat aaatgaaatc
     1621 acaacattta gtaatagacc tcagttttta atcaagtcac ctatagaaag catagattac
     1681 aaaagaataa ttaccgaaaa caaagttgtt tacttagaaa gtaataacca agaaaaaaaa
     1741 gaatccatag atgtattaaa aaactatgat attattatta attgtgtatt acaaaaccca
     1801 acatctccta ttatatttat aaaaaataat gatattgata aaataacaaa aaaaacaact
     1861 atcattgata ttagttgtga taaaaatatg gggtttgaat ttgctagtcc aacttacttc
     1921 gatgaaccat ttattaatat tagtgaaaat attaaatatt actctataga caatacacca
     1981 tctatttatt gggattctgc ttcttatgaa ataactaaat cattaattcc ttttttagaa
     2041 ctatttattt ctaatttatg gaaaaaagat cctataataa aaaatgctat agaaatagaa
     2101 aatggaataa ttaaaaataa aaaaataata gaatttcaga aaagatgata tgaagttaat
     2161 atcatacaac atcgcataaa atatatttta tcttttattt ttggaaaaat taatagtgaa
     2221 gaatctgata aaaaaaacat taaaaatcat actaacacaa attacaaaaa tatcacctaa
     2281 actatcaagc aaattacatt attattatgt ttttcataaa aaactaaata taaaaaaacc
     2341 gtccacattt aatgaaaaaa tacaatggtt aaaattaaat atatatccta gagataagtt
     2401 atatactatt tgtgcagata aatataaagt tagagattat gttgttaata aagattgtag
     2461 agaaatatta aatgaattaa tcgcttcata tgatagcatc aatgaaattg actgggatac
     2521 tttacccaat ggatttgtta ttaaatgcaa tcatggtgcc ggttataaca taatatgtca
     2581 aaataaaaaa aatcttgata ttaatatgac taaagaatta ataaagagat ggctaaatga
     2641 agattattgg ctaaaaatgg cagaaactca ctacaaaaaa atccctaaaa aaattattat
     2701 tgaaaaatta atcaaaaatg aaaaaaatca gttacccgaa gattacaaag tctattgctt
     2761 taatggaaag cctgagtttg taatgatttg ttgtgaaagg aatacaggtc atcctaaata
     2821 ttattttgta gataagaatt ttaatttatt gccttttggt tcagcctatg aaaaaaatcc
     2881 taatataaaa ttagacaaac ctttaggttt tgatgaatta tttatttatg caaaaaaact
     2941 atgccaggat tttgtttttg taagagcaga tttttatatt aatgatggta atattatttt
     3001 tggtgaatta acttttactc cagcagctgg acttgataat aaaattagta atgtgttatt
     3061 acctaatggt gatgcaatta tgggagaata tttaaaacta ccaaatctac cttatcacaa
     3121 taaaggttaa ttatacatat tatgtataga aacttcgtag aaaagctaag ttattttttt
     3181 agtaataatt tatctattat aaaaaattca tcttggataa taattgaaaa actattatct
     3241 atttttgggc taatatttat tacatcttat gtagcaaagt atataggccc agataatttc
     3301 ggaaaattat cttacgctat atctatattt attattattc aaacagtatc aatgttaggc
     3361 acggacacca tattgtttaa aagaataagt aaaaatagag attccggtat tagtctaatg
     3421 ctctcgacaa tagaattaag aattttatta tatatattaa tatctattct ttttacttta
     3481 tatagcatat ataccacaaa agatcatata acaataattt ttacaatatc ggttgctttc
     3541 tcttatttgt ttctagctat tgaccattat gctatttatt ttaatgcaat attacagtca
     3601 aaaattaatg ttctttttaa cattataggt atttcaatat cattattatt acgatattta
     3661 atcgttctat ttgagttaaa tataatatat ttttcaattc caattatcct tacgacctta
     3721 ttacctttta tatttagatg tatctatttt catagaaaaa tagcgctaaa gaaaataaaa
     3781 aaaaggaaac catatattat ttatttaatg agacagggtt tacctatatc aatatcaaat
     3841 gtttcaattt cagtttattc aaaaatatct caaattttcc tttctttatt aatatctaac
     3901 tatgctctag gaatatatac agtagcatca acactagcct atgcttggct atttttacca
     3961 caagcagtaa taacatctta ttttacaaaa atatatagta aaaataataa aaacgaaata
     4021 ttacaattaa ctgcttatct taatttattt attattttta tatctgtatt atacttaata
     4081 tttatatatt tttttggaga aaaaataatc ttaatattat atggaataga ttacattgag
     4141 tcagctaata taatttttta tttgtcaata gcctctgttt tctctagttt aggcattgtt
     4201 tcatatcgct atatagttgc tttttctggg tattcatttc tttctaaaaa aatgtttttt
     4261 acttcttttt tgggtattct gttaacgtat gttttaataa aagaatatgc tattactggt
     4321 gccgtattag ctagtatttt aattgaaatt gtttccctta cagtatttaa ttattttttt
     4381 aaaaataaat tagttttttt tatgcatata aaaacaattt atataatttt taatttttca
     4441 aaaaaacata taaaagagta aaattaaaat gaacaaatta attgaatcac atagaactcc
     4501 tttagtatct gtaataattc ctacatatgg aagacctcat tttctagaaa aagctttaga
     4561 tagtatatta tctcagtcat ataaaaatat agaaataata gttgtagatg acaaccctaa
     4621 taattcaaac cataaaaaaa caaaagaaat aataaataaa tataaatata aatgtaaaat
     4681 aatatatatt tttgatggta taaacgtagg tggatcttta gctagaaata aaggaatcat
     4741 tgaatcaaaa ggagattata ttactttttt agatgatgat gacatatatt taaaatataa
     4801 agttgaaaag caggtagaac atattataaa taataatctt gatgttagtg tatgtaatat
     4861 gttttttaaa aaaaatgata aatttctaga aattagcaat tgttatagcc gagtcagatc
     4921 tattcaagag tttatactaa atggtaatgc ttacacacct atgattatga gtaaaaggaa
     4981 atcattatta gatgtaggat tatttactca ttctccacga taccaagacc atatattaat
     5041 gttaaaatta ttcatggcag ataaaaaaat aaaatcactg aatgaaaaac ttttcattca
     5101 taacgatcat gatgaacaaa gaataactta tagcaataag tctgaagaag cttataaaat
     5161 aaggcaatta tatgagaaga aaaatttata tagattagat gttaaagaaa aaaaacaata
     5221 taaaataatt gaacttctta ttactgccaa aatattacga gcaaaaaaaa actattttaa
     5281 ctcattgaaa aatatatttt tctcattcat gtatataaca aacaagaatg acctatataa
     5341 gataataaaa acattaatta gaatacatat ttttccaaat aaaaacatat aaaatgaata
     5401 tgaatacttt aaaatataat ccttttgtta cagccttctt tttattatat actttatatt
     5461 caattaagag tggttttttt tcattagcca taaaaaatga atttatcttt cagataaaag
     5521 atattgtaaa tatatctata attagtatag ccatattttt ttccttttta ttatcaaaaa
     5581 ataaaactaa aatatattta ataatattta atatattgtc tattgttgga ctactttact
     5641 ttcataataa attttattta ggtttcttac taacaatatc aatgctaaca ttgacatcta
     5701 aaataaaatg ggaagttatt attaaaataa ttatcttttc taatgcaata actattttat
     5761 ttttatgctt ttctgtatat tttagtgaat attacttttt agaggatgat agatttggaa
     5821 gaaggtttac tgcaggattt gataatccta atacattagg acaatatatt ttaatgctat
     5881 ttactgtaat atctcttttc ttagagttga aagttaaaaa tcctagcact agattaatta
     5941 ttactacttc aatatttatt attatattta caatattata tttaacttac tcaaggacaa
     6001 gtttaatttt atcttctcta ttttatataa taataatata ttctatacta ctaaagaaaa
     6061 ataatcattt tttacctaga ataaaattaa aatatttaat tttattctct tctttatttt
     6121 ttatttttct ccagttttat tttataataa attttaaaga gaacaatttc ctttatacgg
     6181 ttaatgatat tttaactagt cgcctctggt ttggaaatat tttatatagt tctttaggtt
     6241 ttccaaatct attaaatgga ataaatatcg aaaaatattc ccctattgat ttctatttta
     6301 tacaaacaat atattctatt ggattaatac ccttcatttt tttatacttt ttagtaataa
     6361 aaaatttatt taatacaaat attacatctt taatgggata tacgcttctt attatgctat
     6421 tagagacaat gactgaaaca tatttttctg taccatttta ttcaatagca ctatttatta
     6481 tctttagtaa gaaaatataa aatgaaaact ctattattct gcataaccga tataacaact
     6541 ggtggtggta ctgaaagagc attaatcacc ctggcgaata aattagctaa cagtaatttt
     6601 gatatacata tttattctct ttataaaaat aatccagaac ctttatttat tataaataag
     6661 aatgtaaaaa tatatttttt agaaaataaa acccatatat ttaaattaat taagatagca
     6721 aaaattgcaa aaaaacatac ttcagcaata aaaatcatta tatcgatggg gagattatcc
     6781 attgaaactt ctctattatt caagttattt aatataaaga atttattctt atcagaacat
     6841 acatctttcg aatcttatag tcctataatc agatctctta aactattaag ctatagattt
     6901 gcagataaga ttatagtatt aactgaacat gacaaattat tacttaataa taaatatcat
     6961 ttagataaaa tttatacgat acctaatatt aatccatatg aagaatattc gtataatctt
     7021 aattataatg ataaagaaaa tatagctatt gctataggaa gattgtgcta tgctaaaaat
     7081 tttgaaagac taataaactt atggagtaaa gctataacaa aaaactggaa attattaatc
     7141 attggtgatg gtaatgataa aaaaaagtta gagaaaacta ttagtaaaag tggagtaaaa
     7201 aatatagaaa taataccatt tacaaaaaac attacttcct attacaaaaa atcaaaatta
     7261 tatttaatga cgtctcgata cgaaggttta ccaatggttc taattgaatc tcaacacttt
     7321 ggtgtaccta ttatctcatt taattgtaaa acaggaccat cagaaatcat aaaaaataat
     7381 tataatggtt tcttagtaga ctactataat gattcactat ttatagataa gttaaattat
     7441 tttataaaaa atgaatccgt acagagaaaa ttatctatta actctcctat agtctcaaat
     7501 gattactcca gtaaaataat tattaatctg tgggaaaaca ttttatctaa aaataaatct
     7561 tatgaaaaat aaaacaattt gtgttgttat acctatttat ataaatacta atattcatct
     7621 atttaagaaa gctattgaat caatactcca acagtcacat ccagcagatg aaatcttaat
     7681 tatttgtgat ggtcttgtaa ctactcaatt aaataatctt ataaaaatat tttcgaataa
     7741 atattcatat ataaaaataa ttcgatacga ttataataaa ggtccaggct atgcaagaga
     7801 ttatggaatc aaatgtactt cttgtgatta tatagctata atggattccg atgatgtatc
     7861 tacaatagac agattagcaa tacaatatga atttatgcag aaaaataacg acatatcttt
     7921 gtgtggtgga ttgataagag aactagatga taagaatcat aaaacttcaa taagaaatgt
     7981 cccaacatcc cattcagaaa tactaaaaac aattaaaata aaatctccta taaacaatgt
     8041 aacagctttt tttcgtcgta aagattattt agaaactgat ggatatgcac atattagaag
     8101 ctctgaagat tatgctcttt gggctagatt aatatataaa gaaaagaaac tacaaaacct
     8161 tccacatata tttgtcgatg tcaacttcga tatagattca ttatccagaa gaactggact
     8221 gactcatttt aaaaatgact tattcacaca aaaagaatta ttaaatttta atttaataac
     8281 tcgatatcaa ttttataaaa atattttaaa atactttata ttccgttgtg ttttgcataa
     8341 aaatataaaa aaaatactat ataaatatta tttaaggaaa taaaattaac atgtataaag
     8401 tcaccatctc cggtaccggc tatgtcggtc tatctaacgg cattctgctt tcccaaaaca
     8461 acaaagtcgt tgcgttagat atcaataaag aaaaagtcga attacttaac aaaaaaatct
     8521 ctcctatctc tgataaagaa attgaagagt ttttagccac taaagaactc gattttacgg
     8581 caacattaga taaagaattc gcttataaag atgcggactt tgttattatt gccacaccaa
     8641 cagattatga tcctaaaact aactacttca acacctcttc tgtagagtct gttatccgtg
     8701 atgttttaga atataatcca aacaccacca tgattattaa atcaacgatc ccggttggtt
     8761 ttactcaaga aatgagagaa aaatataata ctaacaatat tatattctcc cctgagtttt
     8821 tacgtgaagg ccgtgcactt tacgataact tatacccttc tcgtattgtt attggtgaac
     8881 aatcagcaag agcacaaaaa tttgctgatc ttttacaaga aggtgcattg aagaaagata
     8941 ttgatgtact tttcacagac tctactgaag ccgaagcaat caaactgttt gctaatacct
     9001 atttagcact acgtgtcgct tattttaatg agctagatag ctatgcccaa gcttatggtt
     9061 taaatgcaaa acaaattatc gaaggggttt gtttagatcc gcgtattggt aatcattata
     9121 acaatccatc attcggttat ggcggttatt gcttaccaaa agatactaaa caattgttag
     9181 caaactatga cgcggtacct aataatatta ttagtgccat cgttgaagct aatcgcacac
     9241 gtaaagattt tatcgcagat tccattatcg ctaaatcacc taagattgtt ggtgtatatc
     9301 gcttaattat gaaatcaggt tctgataatt tccgctcttc ttcaatccaa ggcattatga
     9361 aacgtattaa agccaaaggg attgaagtag tcatttatga accagaaatg aaagaagata
     9421 ccttctttaa ctcaagatta atccgtaatc tggatgagtt caaaacaatg tctgatgtca
     9481 ttattactaa tagaatggcc tcagcactaa acgatgttga agaaaaagtt tatactcgtg
     9541 atctctttgg taacgattaa tctcatttca tatgctcaat atcgtcttat ttgaaccgga
     9601 aatcccaccc aatacgggta atattatccg cctgtgtgca aatacaggct ttcatcttca
     9661 tttaatccag cctcttggtt ttacttggga tgataaacgt ctacgtcgtg cagggcttga
     9721 ttatcgtgag tttgctgata ttaaacaaca tcatgattat tatgcttttt tagaaagcga
     9781 aggattaagc cctgataatg cacaatcacc aaccggtgct cgtgtatttg ccttaacgac
     9841 aaaaggtaca ccagcacata gtaatgtcag ctatcaggat ggagattatc ttctttttgg
     9901 tcctgaaaca cggggtttac ccccttatgt gttagataac atgccaacac agcagaaaat
     9961 cagagtccca atgcttgcag atagtcgtag tatgaattta tcaaatactg tggctgttgt
    10021 tgttttcgag gcttggcggc agttaggtta ccctggggca ttattaaaag attgattagt
    10081 atgatgttta aggccattaa tacctagcct gctaagctta attatcttct atgaaaataa
    10141 aaaggcgcta attgaattaa ttagcgcctt tttattttta atattgtcta tatcacgtca
    10201 aactaaacag taagatgcca acaagttaat cattctttta tagcaaacta cctttattaa
    10261 atcccatcac cgcattcaaa gcctggtacc acaccgttaa agtgctgatc catctcgaaa
    10321 gagggcatgt cacttgaggg tttacccaca attctggcag ggactccagc aaccgtagta
    10381 tgtgcaggaa cggcatgtaa aacgacagag ccagcaccaa ttttagcacc acgaccaatt
    10441 tcaatattgc ctaaaatttt agcgcctgca ccaatcataa cgccttgtcg cactttaggg
    10501 tgtctatcgc cacacgtttt tcctgttccg cctaatgtca ctgattgtaa aatagagaca
    10561 tcattttcaa cgatagcagt ttcaccaata acaatacctg tggcgtgatc aagcattatg
    10621 ccatggccaa tacaagcagc aggatgaata tcaacaccaa aagtcactga tatttggttt
    10681 tgtaagtaaa tcgctaatgc ttgtctttct tgagcccata accaatgggc tatacgataa
    10741 gcttgtaagg catgaaagcc ttttaaataa agcaacgggg ttgaatattt atcaacagcg
    10801 gggtctcgta agcgaacagc cgaaatatca taagcggctg attcaatcat tgaaacatcg
    10861 ctacgataag cttcttcaac aatttctcgc acagcaatag ccggcatggt ttgtgtcgct
    10921 aatttatttg ccagcataaa actaagggca ctacctaagt tttcatgctt aagtaatgtc
    10981 gcatggaaga aacttgccaa cataggctcg caatctgcca acgctcttgc ttcactttta
    11041 atatgatccc aaactcgttt cagttcttct agcgacatac tgactctcat tctttttata
    11101 ttaaagcaaa taatgaaaat gaatcaattt gcaaaataat taaggtaatg tgctgtctat
    11161 cccgtctttt gttgctcgcc ctaataaggc agaagcagcc tctttgacat ctttatgttg
    11221 atataaaatt tgataaattt gttctgtgat aggcatttca acaccgactt tttcagctaa
    11281 tgctcgcact tcttttgtat tgcgatagcc ttcaacaact tgccctattt tgtcttgcgc
    11341 ggtatcaacg tcaatacctt gacccagcat cataccaaat cgacgatttc gcgactgatt
    11401 atcagtacac gttaagacta aatcacccaa ccctgccata cccataaaag tcgccgcatc
    11461 agcaccgagt gctttaccta atcggctcat ttcagcaagg ccacgtgtaa ttaaggcggt
    11521 acgggcattg gcaccaaagc ccataccatc agacatacca gcaccaatcg cgataacgtt
    11581 cttaacagcg ccacctaatt gcacaccaat aaagtctgga tttttataaa cacgaaagct
    11641 tttaccacaa tgaaaaagct gttgaagctc ttcacaataa ccattatctg ttgaagcaac
    11701 agaaatcgcg gtaggtaagc ctgcagcaag ttctttcgca aaagtagggc ctgataacac
    11761 agctaacggg atttcattac ctaatacttc acgcgcaaca tcttgcaata aacggcctgt
    11821 atgtgcttca agcccttttg ttgcccaaac tatacgtgag tcttggcgta aaaaaggttt
    11881 gatttgttgt aatacatcac caaaaacatg gctgggtaca acaacgagaa tattacggct
    11941 agcttctatc gctttttgta atgaagcttc catatataaa ctatcaggaa aggaaacatc
    12001 cggcagaaaa gcctgattac atcgtgcttg ttctaaagca gcaacgtgtg caggatcatg
    12061 cccccaaagt acaacattgt ggccattacg tgctaaggta atcgctaaag cggtgccgta
    12121 agatccggca ccgagaacag tcatagaagc gttgttcatt aagcttcttg agctccttcg
    12181 ttagttccat cttgttgctg ttgtaaatag ttcataaaca acgcatcaaa atttactggc
    12241 gctaagttta attgtgggaa tgtaccacgg ccaactaaat tggtaataca ctcacgagca
    12301 taagggaaca gaacgttaag gcaatatgca cctagccaat gtgccatatt gccttaagtt
    12361 tgttcttatg ctgtgagtgt attacaattt agttggcgtg gtacattcca caattaactt
    12421 agcgccggta attttgatgc gttgtttatg actattcaca gcacagatgg aactacaaga
    12481 ctagagctag gaaacgctct tacgttctcg gtgcggacta cgcccct
//
