LOCUS       KY710729               17400 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2658 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710729
VERSION     KY710729.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2658"
                     /serotype="O62"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..17400
                     /note="O antigen gene cluster"
     CDS             1632..2525
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlD"
                     /product="rmlD"
                     /protein_id="AXY99983.1"
                     /translation="MKIILFGKNGQVGWELQRSLAPLGTVIALDSKSIEYKADFLFPS
                     ELEKTILSIKPDIIVNAAAYTNVDKAETDDINAFKINAESVEVIAKTAEKIGALLIHY
                     STDYVFDGTNDIPWLETDIPAPLNIYGKSKLAGELAIINNCKKYLIFRTSWVYASKGN
                     NFAKTMLKLGKSHDSLSIIDDQYGAPTSAELLADCTAHAIKSASDNESLYGLYHLTAK
                     GTTTWFEYASLIFKYAQEYSLPLAVKNLTPIPAKEYPLPAFRPHNSRLNTQKFETNFK
                     LELPHWKFGIYRMLNEILDNK"
     CDS             2552..3430
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99984.1"
                     /translation="MKKYKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGIHLQYKVQPSPDGLAQAFILGEDFIGS
                     DNCALVLGDNIFYGHDLPKQLEQAIQRDQGATVFAYHVNDPERYGVVEFDQNKVAISL
                     EEKPLKPKSNYAVTGLYFYDNDVINIAKKLQPSPRGELEITDINKAYLEQHKLSVAMM
                     GRGYAWLDTGTHKSLIDASNFIQTIEERQGLKVSCPEEIAYRKGFINKEQVRILAEPL
                     SKNDYGKYLLRMIQSD"
     CDS             3433..3978
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="rmlC"
                     /protein_id="AXY99985.1"
                     /translation="MNIIKTEIPDVLILEPKVFGDHRGFFYESFNQKIFEDAIGYPVN
                     FVQDNHSKSSKGVLRGLHYQKAPHAQGKLVRCVYGEVFDVAVDIRPESNTYGKWVSAI
                     LSAENKRQLWIPEGFAHGFLVLSDKAEFLYKATNFYYPEYEDHIIWNDDNLNINWPVI
                     DNLIISEKDQRIKNLMPREIL"
     CDS             3975..4949
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXY99986.1"
                     /translation="MIFNLLRRINNYVLLEFNTIYDYVKFKKNFTKLKNANNDKTKLS
                     CWILQDKHRIEKAFTLPNPRFGFGKDVIERISKNLNIYKSKYGVDNIYQIGISALFSY
                     KEFHQDNNKEVPDFYTLNIKQLDKQDISIIEKKAGYYIPEKLDEISLKAFKKFAQSRH
                     SCRNFQKNKEITDSVIRDIIEISITAPSVCNRQHWRIHFFTGDKMEKILSFQNGNSGF
                     TENIPMIAVITSDIRAFYTPNERNQPYIDGGIFAMNVMYAIHSVGLESCPLNWCNSYK
                     QEINFDKLNLIEKNERIILILAMGYSTDSALYAKSPRLPIDNFYKLHK"
     CDS             4958..6481
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99987.1"
                     /translation="MRLKNSINNTIYSLANIILVTILNIIYRTIFIRILGAEYLGVSA
                     ALTNIITLLSLTELGFSQAISYHLYKPLLEKKYNKTYGIMCYFKKVYFNIGILLLILG
                     CILLPFLEIIIPTQISTKELNIAFFLFILNSSITYFWGYKRTLIIADQKNYKIIPYIS
                     LFQTVELVLKSTILFLTESFIITLLVQLIIKLIENIAINKYITDKYKYIFKSGNNILD
                     KYDILSIRRKTIGVMYHKFGDVFINGTDNIIISMFVGVSILGIYSNYAMIIALLTSFI
                     LVFFNSLISSFGNLIIENKRKSENTFYIIQLISIYLFGMIGTIYFLTINDVIKLWIGS
                     QFILPQSIVSLIVINFFLLGLRIPLNVVKTAGGAFEQDKYAPIIQGIMNLVLSILLSK
                     NYGIAGVLLGTLISTILIPLWVQPYIVYKYIFNTDIRKYIINTIKLLFVNIFIFFCIY
                     QIAILLPILDIDNKYLELALKVTTIFFSYSFIILLIKRRTFITLFKSIISIRNEKIS"
     CDS             6465..7412
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99988.1"
                     /translation="MKKLVSILVPCYNSESLIQKLLQSILSQDYPYIEVIVINDGSTD
                     NSLSVIYSYLNEFEKKGYILKIISQKNAGQASAINNALQYFSGDYLSWIDADDWLEPS
                     AIADKVKYLERNLEYGLVRSDVSIYFYPNYKIASYKFSDKMDFSNENIFDDLILENNV
                     YFCPGGYLVRTTALKKVLPNLKIINSRAGQNWQILLPLTLEFKCGYINKPLFNYLIRE
                     NSHSRKEKDKIQELKKLQSHEELLKKIIYSLKLNNKKKYAKIIEKKYLDKKISIYYKY
                     NDIKNILKLTLKRKNIKSIIKSILVILKLTLKKIKYFIN"
     CDS             7430..8554
                     /note="orf08"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf08"
                     /product="hypothetical protein"
                     /protein_id="AXY99989.1"
                     /translation="MKKIGVLNFHYSNHNYGAVLQAASLANILKENNFDVEHINYVPK
                     QKVSLSRYIKIKLRFLAKIFVKNRSNITFNVGNDNIFEDFRDKWLARSKKTYHHPDDF
                     YNFNIEKQYSTIIVGSDQVWRPKMYLNPEDFKVYFLGFANNTVNKVSYAASFGVDNWE
                     YKQTDNITKEVKKYIKDFNYISTRELTGINICKTIFDVSSISVLDPTLLVGKSFFDKI
                     INESNVDTNNSDLVYYKLDMNDAFLSKIDDICSNLNLTSSENIYFYKENKKYKYNSVP
                     AWLNKIKNSKFIITDSFHCVCFAILFEKEFICCINNNRGLSRLESLFEQLEIKDRLID
                     INELTINNINNISSIDYKKLNRKLNELRQISLDFLLSSIK"
     CDS             8572..9456
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99990.1"
                     /translation="MKIPIIVMTRNEDEYLNTCVNSIINTISIDYHIYIIDNRSDSLK
                     QKKILSDLENQHKEKLTVIRNKNNLWILGINATLREIKKTSDSKYFFLTDGDIDFSHC
                     NCNTSPCWMSYLISNMEKNISIGKLGLSLSWDYLLKDDSLQSILNQEKSLYSEQKKIN
                     DLYVSAIDTTATLYRWDWSIENSSQFYPDHMRYLRPELYSCRTPRNITVEHLGWKKYN
                     SLIDDKKSINSKVVCFTIVGGDIKKQILNKASIQNQFFYKLFSYPILKLWTLRRYIKL
                     FIYFFKKGIHSFDGQNSN"
     CDS             9437..10486
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99991.1"
                     /translation="MDRIQIELNKMKPIIKIKKNTIIIIVITFFLSLVASYLSSFSND
                     YKIYSDIYKNTIDQNTLNKIEPLYLLINNLFYSLGFDFSIFIFFISFISLFIKISILN
                     KLTSSSFIFYLLILMYVSSLFIIHEITQFRVSLAIAFGFLCCYKLTNYKKKESILFLI
                     IAILFHYSAILFSLIYIIKNGYRKLKRIDVLIMVSLFFLIPLIIKMTITILIHLNPLF
                     LEYYSNSNQTNSKGLLSITTLLATTFTIINLFLLRSFYSSLHYRVFSFLNIVGLLFLI
                     GLSFSPVLSIRIYELFSFSSFIIISLLYNKVFSLSGNNRYSFYCIRTLLLLLFILISL
                     HRFIAFFIVNPILTF"
     CDS             10496..11398
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99992.1"
                     /translation="MNDYKIYSILVSYNPDIDILLTSLNSMHTQVEKIILVDNSSNNS
                     EKIEEIQHKFTNIHFIKLDKNIGLAQAQNIGIQYSLDEKVDYVILFDQDSIISKSFIN
                     NLVSSSLEIQAKKDTKIAAIGPIFYDPMTMSNYPATIYSGPFIKRINIKDEPIQATFI
                     IASGSLISASVLRDIGKMKNELFIDYIDVEWCLRAKNKGYSVYITPHASMAHTIGDRR
                     IKLLHRTISVHSPLRRYYLIRNSIIISKINYIPIGYKIRELFFNPIRFAVSLYTSKEK
                     LKVIKYTWFAILDGIKGISGPFKH"
     CDS             11414..12571
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99993.1"
                     /translation="MIMIKIVHIQLLPLLSGAQRVSLEELRRLPDDKYKKYLISSAEG
                     PLTQEAKKHNIQCIIIPTLKREISPRNDFLSLLDLIKIIKKERFDIVHTHSSKPGVLG
                     RIAAFLLRTPLIVHTVHGFSFPAAKNKKEYFLYLTMERIGTFCGDSLICLHNNDKNIA
                     IKKLYIKEKNILVIPNGVDTNLYTPLDKKYKNEIRKNLQINNNDISIGMIGRLWEQKN
                     PLLLLNAAIELLHKHSNLKFYFVGDGEYFNLMDDIIKRNELTNKIFLLGWRNDTEIIL
                     NALDIFVLPSKWEGMPLAILEAQSTAVPCVVSDIQGNKEIIVNNKTGILFESNSKSEL
                     IKSIDLLVSNPEKRKEIAQNARNYITENHFINDRINKLDHYYTIKLKNQIR"
     CDS             12693..13859
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99994.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             13871..14374
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99995.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(14445..15002)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99996.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRIYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIKQSSM"
     CDS             complement(15002..15835)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99997.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15914..16930)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99998.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(16930..17400)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     6247 a   2768 c   2702 g   5683 t
ORIGIN
        1 atgctagaac aacatatcga agctgaatta gctcaagatc cggctaacga tctcctttgg
       61 tggcgtcgat taattcgagc tattgataaa tgggctcccc ctggccaacg tttaattatt
      121 gtcacgagtg aaggccggat tatcggtgct caacgtaatg agatgcaagt tgtcagaaac
      181 tttattggtc aatctgataa cgccgatcac cccaaaaaga aaaaatatgg tcgctctgaa
      241 atgctagggc ctttttctgt aagggatggt gaagatcatt accaacttta tctcgtgcgc
      301 ccttcaagta gccctcaatc tgactttatc aacttattat ttgataaacc attgttgtta
      361 ctcatattca ccatgcttat cagcacacca ttattagtat ggctttcttg gagcttagca
      421 aaacccgcaa gacaacttaa aaatgccgct gacgatgtgg ctaaaggtaa cctacgccct
      481 caccctgaac tagaaactgg ccctcaagag tttttagccg caggcacgag ttttaatcag
      541 atgataagtg ctcttgaacg tatggtagag gcgcaacaac gtctgatctc tgatatttct
      601 catgaactac gtaccccatt aacacgctta caattagcca gtgcattgtt acgtcgtcgt
      661 agtggagaga gcaaggagct agagcgtatt gaaactgaga ctcaacgcct tgatggtatg
      721 attaatgact tactagtact ttcccgtaat cagtataaaa acgagttatt acgcgaaaca
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataacg ctaaatttga ggccgaacaa
      841 agtaataaga cactgcaagt cacaacccct ccaggagcct ggcctattta ctgcaatcca
      901 tattctctgg cgagtgcatt tgagaatata gtccgtaatg ctctgcgtta ttcaaattct
      961 cgtattgaag tcgcgtttac tgaacagaac caaggtatca ccattatcgt tgatgatgat
     1021 ggccctggcg ttagccctga agatagagag catatcttcc gaccattcta tcgcactgac
     1081 gaagctagag atcgtgaatc cggtggtacg ggcttaggat tagcgattgt tgaaactgcc
     1141 gtcagccaac acagaggcca tgttaaagcc gatgacagcc cattaggtgg gttgagggtt
     1201 gagatttggt tgccgagagc gggtgcaagt aacaaatcgc caacagctta atagagtaat
     1261 taaactgttg tatcaaaata tcctgccatc tattgaagaa taggtggcat attctttttg
     1321 ttcgcattta ttgatgcaat tcagaatgat ctggctaaat cgatcgctat tccgatattt
     1381 atatatctac aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt
     1441 ctactctcag tgcggatttt cgcatattgt taaagtatgc ttatataata aaactcttat
     1501 attacatata gataagggtt tttcgagata aattttggcg ctctgggggc cgtaaaccca
     1561 gtgcggtagc tatgtatatt caatgctata aatataatat attaatatta gggtaattaa
     1621 agaactaaat aatgaaaatt atcttatttg gaaaaaacgg gcaagtaggt tgggaattac
     1681 agcgctctct agctccatta ggaactgtta ttgctttaga ttctaaatca atagaatata
     1741 aggctgattt tttattccct tctgaactag aaaaaaccat tttatctatt aagcctgata
     1801 ttattgtcaa tgctgctgct tatacaaatg tagataaagc tgaaacggac gacattaatg
     1861 cattcaaaat caatgctgaa agcgttgaag taattgcaaa aacagctgaa aaaattggtg
     1921 ctttattaat acattattca acagattatg tttttgatgg aaccaatgat attccttggt
     1981 tagaaacaga tattccagca cctttaaata tttatggtaa aagtaagcta gctggtgaac
     2041 tagctattat taataactgt aaaaaatatc taatatttag aactagttgg gtttatgcaa
     2101 gtaaaggtaa taactttgca aagacaatgc taaaactggg taaaagccat gattcgctat
     2161 caataattga tgatcaatat ggagcaccta cgagtgctga gctattggct gattgcacag
     2221 ctcatgcgat taaaagtgca tcagataatg aatcacttta tggcttgtat catttaactg
     2281 caaaaggaac aacaacatgg tttgaatatg catctttaat atttaaatat gcacaagaat
     2341 attcattacc attagctgtt aaaaatctaa ctcctatccc tgccaaagaa tatcctttac
     2401 ccgcatttag accacataat tctcgtttaa atacacagaa atttgaaaca aatttcaaat
     2461 tagaattgcc tcattggaag tttggcatat atagaatgct aaatgaaatt ttagataaca
     2521 aataaatatc aatacttata gtttacagaa aatgaaaaaa tataaaggta ttattcttgc
     2581 tggtggctca ggaactcggc tttatcctgt cacaatggca gtaagtaagc agttgttacc
     2641 aatttatgat aaaccaatga tctattatcc tcttagcacc cttatgttag caggaataag
     2701 agatatatta attattagta cacctcaaga tacacctcga tttcaacagc tattaggtga
     2761 cggtagccaa tggggtatcc atcttcaata taaagttcaa cctagtcctg acggcttagc
     2821 gcaagcattt attcttggtg aagattttat tggttcagat aattgtgctc ttgttttagg
     2881 tgataatatt ttttatggtc atgacctccc taaacaatta gaacaagcaa ttcaaagaga
     2941 ccaaggtgcg acagtttttg cttatcatgt taatgatcct gaacgctatg gagttgtcga
     3001 attcgatcaa aataaagtag ctatttcatt agaagaaaaa ccactaaaac ctaaaagtaa
     3061 ttacgcagtc actggtttat atttctatga taatgatgtc attaatattg ctaagaaatt
     3121 acaaccttcc ccgcgtggtg aattggaaat cactgatata aataaagcat atctagaaca
     3181 acataagctc tcagtcgcca tgatgggtcg tggatatgcc tggttagata caggaacaca
     3241 taaaagtctt attgatgcaa gcaacttcat tcaaactata gaggaaagac aaggattaaa
     3301 agtttcttgt ccagaagaaa ttgcttatag aaaaggtttt atcaataaag aacaagtaag
     3361 aatacttgct gagcctttat ctaaaaatga ttatggcaaa tacctattac gaatgattca
     3421 gagtgattaa ttatgaatat tataaaaaca gaaattcctg atgtattaat tttagagcct
     3481 aaagtatttg gtgatcatag aggctttttt tatgaaagct ttaatcaaaa aatatttgaa
     3541 gatgcaattg gttatcctgt aaattttgtt caagataatc actctaaatc atcaaaagga
     3601 gtattaagag gattacatta tcaaaaagca ccacatgctc aaggtaaatt agtacgatgc
     3661 gtttacggtg aagttttcga tgtagcggta gatattagac ctgaatccaa tacatatgga
     3721 aaatgggtta gtgcaatatt atcagcagaa aataaacgcc aattatggat tccagaaggt
     3781 tttgctcatg gttttttagt tttaagtgac aaggcagaat ttctttataa agctactaat
     3841 ttttactacc ccgaatatga agatcacatc atatggaatg atgataattt aaatataaac
     3901 tggcctgtaa tagataatct aattatctca gaaaaagatc aaagaataaa aaacttaatg
     3961 cctagagaaa tattatgatt tttaacttat tgagaagaat aaataactat gtattgcttg
     4021 aattcaatac aatctatgat tacgttaaat tcaaaaagaa tttcactaaa ttaaaaaatg
     4081 caaataatga taaaacaaaa ttaagctgtt ggattctgca agataaacac agaatcgaaa
     4141 aagcttttac attacctaat ccacgatttg gttttggaaa agatgtcatt gaacgaatat
     4201 cgaaaaatct aaacatatac aaaagtaaat atggtgttga taatatctat caaataggaa
     4261 tcagtgcttt attttcatat aaagaatttc atcaagataa taataaagaa gttcctgatt
     4321 tctatacttt aaatataaaa caacttgata aacaagatat tagtataata gaaaaaaagg
     4381 ctggttatta tatacctgag aaattggatg aaatatcatt aaaagcattt aaaaaattcg
     4441 ctcaaagcag gcatagttgc aggaattttc aaaaaaataa agaaataaca gattcagtta
     4501 ttagagatat tattgaaata tctattacag caccttccgt atgtaatcga cagcattggc
     4561 gtatccattt ttttacaggt gataaaatgg agaaaatatt aagtttccaa aatggtaact
     4621 caggtttcac agaaaatatt ccgatgattg cagtaataac aagtgatata cgagcatttt
     4681 atacaccaaa tgaacgaaat caaccttata tagatggcgg aatatttgcg atgaatgtta
     4741 tgtatgcaat acactctgta ggtttagagt catgtccatt aaattggtgc aactcatata
     4801 aacaagaaat aaattttgat aaattaaatt taatcgaaaa aaatgaaaga attatcttga
     4861 tcctagctat gggatattca actgattctg ctctatatgc aaaatcacct agacttccta
     4921 ttgataattt ctataaactt cataaataat attgacaatg agattaaaaa actcaataaa
     4981 taatactatt tattcattag caaatataat tcttgttacc atattaaata ttatttatag
     5041 aactatattc attagaatac ttggcgccga gtatttaggt gttagtgcag cattaacaaa
     5101 tattataacg ctactatcac ttaccgaact tggtttttct caagcaatat cttatcattt
     5161 atacaaacct ttgctggaga agaaatataa taaaacatat ggaataatgt gctactttaa
     5221 aaaagtatac tttaatatag gtatattatt attaatactt ggctgtatct tattgccatt
     5281 tcttgaaatt ataataccga cccaaatatc aacaaaagaa ttgaatattg cattttttct
     5341 atttatttta aatagctcaa ttacatactt ttggggatat aaacgaacat taataattgc
     5401 agatcagaaa aattacaaga tcattcctta tatttcgtta tttcaaactg ttgaattggt
     5461 gttaaaatca accattttat ttttaactga aagctttatt attactctat tggttcaatt
     5521 aattattaaa ttaattgaaa acatcgctat taataaatat attacagata aatataaata
     5581 tatttttaaa tctggaaata atatattaga taaatatgat atattatcta tcaggagaaa
     5641 aactatagga gtaatgtacc acaagtttgg tgatgtattt ataaatggta cagacaatat
     5701 tattatatca atgttcgtgg gtgtctctat tcttggtata tattctaatt atgcaatgat
     5761 tattgcatta ttaacatctt ttattttggt ctttttcaat agtttgatct caagctttgg
     5821 taatctgatt attgaaaata aaagaaaaag tgaaaataca ttttatataa ttcagttgat
     5881 atccatttat ttattcggaa tgatagggac aatatatttt ctgacaataa atgatgtaat
     5941 aaaattatgg ataggtagcc aatttatttt accacaatca atagtttcat tgattgttat
     6001 taactttttt ttattaggtt tacgtattcc cttaaatgtt gtaaaaactg caggaggggc
     6061 attcgaacaa gataaatatg cacctataat tcaaggaatt atgaatttag tattatctat
     6121 tttattatct aaaaattatg gtattgcggg agttctgctt ggtacattaa tatcaactat
     6181 tttaatccca ttatgggtac agccttatat agtttataaa tatattttta atacagacat
     6241 aagaaaatat attatcaata ctataaagct attatttgtt aatatattta tattcttctg
     6301 catatatcaa atagctatat tattgcctat attagatata gataataagt atctagaatt
     6361 agctctgaaa gtaacaacca tattcttctc ttatagtttt ataatattat tgattaaaag
     6421 aagaacattt ataactctat ttaaaagcat aatatcaata agaaatgaaa aaattagtta
     6481 gtatcttagt accatgctat aatagtgaat cgttaataca aaaattatta caatccatat
     6541 tatctcagga ttatccttat atagaggtaa tagttattaa tgatggttca acagataatt
     6601 ctctatctgt aatttattct tatcttaatg aattcgaaaa aaaaggatat atattaaaaa
     6661 ttataagtca aaaaaatgca gggcaagcta gtgcaatcaa taatgcatta caatattttt
     6721 ctggagatta tttgtcatgg attgatgctg atgattggtt agagccttcg gctatcgctg
     6781 ataaagtaaa atatcttgaa aggaacttag aatatggctt agttagaagc gatgtatcga
     6841 tatattttta ccctaattat aaaatagctt cttataagtt tagtgataaa atggattttt
     6901 ctaatgaaaa tatatttgat gatttaattt tagagaataa tgtttatttt tgtccaggcg
     6961 ggtacttagt cagaacaact gcactaaaaa aagttcttcc aaacctaaaa atcatcaatt
     7021 caagagcagg gcaaaattgg caaatattgt tacccctaac gctagagttt aaatgtggat
     7081 atataaataa accattattt aattatttaa ttagagaaaa tagtcattct agaaaagaga
     7141 aagataaaat acaagagttg aaaaaattac aatcacatga agaactttta aaaaaaataa
     7201 tatatagctt aaaactaaat aataaaaaaa aatacgcaaa gataatagaa aaaaaatact
     7261 tagataaaaa gatatctatt tactacaaat ataatgacat taagaacata ctaaaactca
     7321 cactgaaaag aaaaaatata aaatcaatta taaaatctat attagttata ttaaaattaa
     7381 cattaaaaaa gataaaatat ttcattaact agaagattaa ggaaaataag tgaagaaaat
     7441 tggtgtttta aactttcatt attctaatca taattatgga gctgttttac aagcagcttc
     7501 tttagctaac atactaaaag aaaataattt tgatgtagaa catattaatt atgttccaaa
     7561 acaaaaagta tccttaagta gatacataaa aataaaatta agattcttgg ctaaaatttt
     7621 tgttaaaaat agaagtaata taacttttaa tgttggaaat gataatatat ttgaagactt
     7681 tagagataaa tggttagctc gaagtaaaaa aacatatcat catcctgatg acttttacaa
     7741 ttttaatata gagaaacaat attcaactat aattgttggt agcgatcaag tatggcgccc
     7801 taaaatgtat ctcaatccag aagactttaa agtttatttt ctaggattcg ctaataatac
     7861 agtcaataaa gtttcttatg cagcaagttt tggtgtcgat aattgggagt ataagcaaac
     7921 agataatata actaaagaag ttaaaaagta tattaaagac ttcaattata tatcaactag
     7981 agaattaact gggattaata tttgcaaaac aatttttgat gttagctcta tatctgttct
     8041 tgatccaaca ttattagtag gtaaaagttt ttttgataaa ataattaatg aaagtaatgt
     8101 agatacgaat aactcggatt tagtttatta taaattagat atgaatgatg cgtttttatc
     8161 taaaatagat gatatttgtt caaatttaaa tcttactagt agtgagaata tttattttta
     8221 taaggaaaat aaaaaatata aatacaatag tgttccagca tggttgaata aaataaaaaa
     8281 tagtaaattt attattactg attcatttca ctgtgtctgt tttgctattc tttttgaaaa
     8341 agaatttatt tgttgtatta ataacaaccg aggattatct cgactagaaa gtctttttga
     8401 gcaattggaa atcaaagaca gactaattga tataaatgaa ctgactatta ataatataaa
     8461 taacatttca tcaatagact ataaaaaatt aaatagaaaa ttaaatgaac taagacaaat
     8521 atcattagat ttcttattat catcaataaa ataaaaataa gctaataatt catgaaaatt
     8581 ccaataatag taatgacccg taatgaagat gagtatttaa atacatgcgt taattccatt
     8641 ataaatacta taagtatcga ttaccatatt tatattattg ataataggtc agactcatta
     8701 aaacaaaaaa aaatactatc tgatttagaa aaccagcata aagaaaaatt aacagttata
     8761 agaaacaaaa ataacttatg gatattagga atcaatgcaa cattgcgtga aataaagaaa
     8821 acttcagata gtaaatattt ttttttaact gatggtgaca ttgatttttc tcattgcaat
     8881 tgcaatactt ccccttgttg gatgagttac ttaattagta atatggaaaa aaatatatct
     8941 atcgggaaat taggattatc actatcgtgg gattatcttt tgaaagatga ttccttacaa
     9001 agcattctaa atcaagaaaa aagtttatac tctgaacaga aaaaaatcaa tgatctttat
     9061 gtttcagcta ttgacacaac agctacatta tatagatggg attggtctat tgaaaactct
     9121 agtcaatttt atccagatca tatgagatat ctaagacctg aattatattc ttgtagaaca
     9181 cctagaaata ttactgttga acatttaggt tggaaaaaat ataattcact tattgatgat
     9241 aaaaaaagta tcaatagtaa agttgtatgt ttcacgattg taggtggaga tataaaaaaa
     9301 caaatactaa ataaagcatc aatacagaat caattttttt ataaattatt ttcttatcct
     9361 attttaaaac tatggacatt aagaaggtac attaaattat ttatttattt tttcaaaaaa
     9421 ggaatacata gttttgatgg acagaattca aattgaatta aataaaatga agccgattat
     9481 aaaaataaag aaaaatacta ttataatcat tgttattact ttttttcttt cattagttgc
     9541 atcttatctt tcgtcattta gtaatgatta taaaatatat tctgatattt ataaaaatac
     9601 catagatcaa aacactctaa ataaaataga gcccctttat ttgttgataa ataatttatt
     9661 ttattctctt gggtttgact tttctatttt catatttttt atatcattta ttagtctatt
     9721 tataaaaata tctattttaa acaaattaac atcttcttct tttattttct atttgctaat
     9781 tttaatgtac gtatcttctc tttttataat tcatgaaata actcaattta gagtatcttt
     9841 agctatcgca tttggatttt tgtgttgtta taaattaact aattataaaa agaaagaatc
     9901 aatattattc ttaataatag ctattttatt tcattattca gccatccttt tttctttaat
     9961 ttatataatt aaaaatggat atcgaaaact aaaaagaata gatgtgttaa tcatggtatc
    10021 attatttttt ttaattcctt taattataaa aatgaccatt actatattaa ttcatctaaa
    10081 cccacttttt ttagagtatt actccaatag caaccaaaca aacagtaaag gattactttc
    10141 cataacaact ttgttggcaa caacctttac aatcataaat ttatttctac taagatcttt
    10201 ttattcatcc cttcattata gagtcttttc ctttttaaat attgtcggtt tattgttcct
    10261 tattggtttg tcattttctc cggttctgtc tattagaatt tatgagttat tttctttttc
    10321 tagctttatt attatttcat tattatacaa taaagtattc tctttatctg gaaacaatag
    10381 atattctttt tattgtatta gaacattact ccttttatta ttcatattaa tctcattaca
    10441 tagatttatt gctttcttta tagtaaatcc tatactaaca ttttaaggtc aaaaaatgaa
    10501 tgactataaa atatattcta ttttggtaag ttacaatccc gatatagata tattactgac
    10561 atcattaaat tcaatgcata ctcaagttga aaaaattatt ctagttgata atagctccaa
    10621 taattcagaa aaaatagaag aaatacaaca taaatttact aatattcatt ttataaaatt
    10681 ggataaaaat attggattag cacaagctca aaatattgga atacaatatt ctttagatga
    10741 aaaagttgac tatgtaattt tgtttgatca ggatagtata atatcgaagt cctttatcaa
    10801 taatcttgta tcctcaagtt tagaaattca agctaaaaaa gatactaaaa tagcagctat
    10861 tggtccaata ttttatgatc caatgaccat gagtaattat ccagcaacaa tatattctgg
    10921 tccatttata aaaagaatta atatcaagga tgaaccaata caagctacat ttattattgc
    10981 ttcaggcagt ttaatttcag cttcggtatt aagagatata ggtaaaatga aaaatgagtt
    11041 atttattgat tatatcgatg ttgaatggtg tcttagagca aaaaataaag ggtattctgt
    11101 ttatataacc cctcatgcat ctatggccca taccataggg gatcgacgaa taaaacttct
    11161 tcatagaaca atatctgtcc atagtccttt acgaagatat tacttgatta gaaatagtat
    11221 aattatctct aaaataaatt acatacctat tggttataaa attagggaat tattttttaa
    11281 tcctattaga tttgccgttt ctttatatac atcaaaagag aaacttaaag ttataaaata
    11341 tacatggttt gccattcttg atgggattaa aggtatatca gggccattca aacattaact
    11401 cattatataa aatatgatta tgataaaaat tgttcatatt caacttttac cgttattgag
    11461 cggagcacaa agagtgtctt tggaagagtt gagaagatta cccgacgata aatataaaaa
    11521 atatcttata tctagtgctg aaggcccact aacacaagaa gctaaaaaac ataatataca
    11581 atgtataatc attccaacat taaaaagaga aatatcacct agaaatgatt ttttatccct
    11641 tttagactta attaaaataa taaaaaaaga gcgctttgat attgttcata ctcattcttc
    11701 aaaaccaggg gttttaggaa gaatagccgc atttctacta agaacccccc ttatagtaca
    11761 tacagtacat ggtttttcat ttccagctgc taaaaataaa aaagaatatt ttttatatct
    11821 tacaatggaa agaattggta ctttttgtgg agactcatta atttgtcttc ataataatga
    11881 taaaaacatt gctattaaaa aattatatat aaaagaaaaa aatattcttg ttattcctaa
    11941 tggagtagac acaaatctat atactccttt agataaaaaa tataaaaatg aaattagaaa
    12001 aaacttacaa attaataata acgatatttc tatcggaatg atcggtagat tatgggaaca
    12061 aaaaaacccc ttactattac ttaatgctgc gattgaactt cttcataaac actctaatct
    12121 taaattttat tttgtaggag atggagagta ttttaattta atggatgaca ttataaaaag
    12181 aaacgaatta acgaataaga tttttctctt aggttggcgt aatgatactg aaattatatt
    12241 gaatgccctt gatatatttg ttctcccatc aaaatgggag ggaatgcctt tagctattct
    12301 agaagcacaa agtactgctg taccctgtgt tgtatctgat atccaaggaa acaaagagat
    12361 tatcgtcaat aataaaactg gtattctttt tgaaagtaat tcaaaaagtg aactaataaa
    12421 aagtatagat cttttagtct ctaatccaga gaaacggaag gaaattgctc aaaacgcaag
    12481 gaactatatc actgaaaatc acttcataaa tgatagaata aataaattag accattacta
    12541 tactattaaa ttaaaaaatc agattagata atactactct tatttatttt aaattatcat
    12601 attaaatttg tttatagctt attattacct aatactttat atatttaata tttttataat
    12661 ttgattcaaa atattttaga taagagaaaa ccatgaaaat caccatctcc ggtaccggct
    12721 atgtcggtct atctaacggg atacttcttt cccaacacaa caaagttgtt gcgttagata
    12781 tcaataaaga aaaagttgaa ttacttaaca aaaaaatctc gccaattaca gacaaagaaa
    12841 ttgaagagtt tttagcaacc aaagagctcg actttacagc aacattagat aaagaattcg
    12901 cttataaaga tgctgaattc gttatcattg ctacaccaac cgattatgat cctaaaacga
    12961 actatttcaa cacctcttct gtagagtctg ttattcgtga tgttttagaa tataatccaa
    13021 acacgacaat gattataaaa tcaaccattc ctgttggttt taccaaagag atgagagaga
    13081 aatataatac caataatatt attttctccc ctgagttctt acgtgaaggt cgtgcgcttt
    13141 acgataactt atacccatct cgtattgtga ttggtgaaca atcagaaaga gcacaaaagt
    13201 ttgctgacct tttacaacaa ggcgcactga aaaaagatat tgatgttctg ttcactgact
    13261 ccacagaagc cgaagcgatt aaattatttg ctaatactta tctagcactc cgtgttgctt
    13321 actttaatga gctagatagc tatgccgaat cttatggact gaactcaaga caaatcattg
    13381 aaggtgtctg cttagatccg cgtattggca atcactataa caatccatca ttcggttacg
    13441 gcggttactg cttaccaaaa gatactaaac agctgttagc caactacgac tctgtcccta
    13501 ataatattat tagtgcaata gtggaagcta accgcacacg taaagatttt attgcagact
    13561 ctattattgc aaaatcaccc aaaatcgtcg gcgtatatcg tttaattatg aaatctggct
    13621 cggataattt ccgttcttct tctatccaag gcattatgaa acgcattaaa gccaaaggga
    13681 ttgaagtggt gatttatgaa ccagagatga aagaagagac cttctttaat tcaaaattaa
    13741 ttcgtgattt aaatgagttt aaagcaatgt ctgatgtcat tattaccaat agaatgtcat
    13801 cagcactaaa cgatgttgaa gaaaaagttt atactcgtga cctctttggt aacgattaaa
    13861 tcgattttat atgctcaata tcgtcttatt tgaacccgaa attccaccga atacgggtaa
    13921 tattatccgc ctgtgtgcta acacaggctt tcatcttcat ttgatccaac cattgggttt
    13981 tacttgggat gataaacgtc tacgtcgtgc agggcttgat tatcgtgaat ttgctgatat
    14041 taaacagcac cacgattatt atgctttttt agaaagtgaa ggtttaaacc ctgataatgc
    14101 acaatcacca tcaggtgcgc gtgtatttgc cttaaccaca aaaggcacgc cagcacatag
    14161 caatgtcagt tatcaagatg gagattatct cctgtttggc cctgaaaccc gtggtttgcc
    14221 accttatgta ttagataata tgccaacaca gcagaaaatc agagtcccaa tgcttgctga
    14281 tagccgcagt atgaatctgt caaatacggt tgcggttgtg gtttttgagg catggcgcca
    14341 acttggttat cctggtgcat tactaagaga ctgaaatgca aaaagcgctg atcataaata
    14401 atgactagcg ctttttaact tcaaataact aacatgttaa tttattacat tgaggattgt
    14461 tttatttcag caacaacctt cgtgatatat cgacaccctt cccctacatc cttcagtact
    14521 aatgcatgag ctccaatcac cgcattatca ccaatctcta tctcaccgcc gataatcgat
    14581 gagttacaac caatataaac gttattacca acccgaatat ccgctttgcc attcttttct
    14641 tttacaccga ttgtcacacc ttgcattaac gtcacattct cgcctagctg acagtaatta
    14701 gttagtacga tccctatggg atgtggtaat gaaaggcctt tacctatgcg cgttcttagt
    14761 gagatatcgt tgcaatatat acgattgttt ttagcaccaa tacgaatgcc taattttttg
    14821 ataaaacgat ttttagaaaa ataaaagaat tgggcgacac gccaccaaaa tagataacgt
    14881 gaacgttggc agaaaaaaac acggtgataa agtcttatcc aactaaaagg cttgttatca
    14941 ccaatatact cagcgtgtaa acaagccttt agttcagaaa gtgtgacttc agttaatgcc
    15001 attaaatgcc atcaccacat tcaaaaccgg gtacgacacc attaaagtgt tgatccatat
    15061 cgagtgatgg cttatcactc gttggtttac ccacaatgcg agcaggaaca ccagcaactg
    15121 tagtgtgcgc aggaacttca tgtaaaacga ctgatcccgc accaatttta gcaccgcgac
    15181 caatctcgat attgccgagt atttttgcac ccgcgccaat cataacacct tcacgcactt
    15241 taggatgacg gtcaccacaa gttttacctg ttccacctaa cgtcacagat tgtaaaatag
    15301 aaacatcgtt ctctacgata gcggtttcac caatcacaat gcctgttgcg tgatcaagca
    15361 taataccgtg gccaatacgg gcagctggat gaatatcaac accaaaagtc acagaaatct
    15421 ggttttgcag atacaccgct aacgctttgc gatcttgatt ccataaccaa tgcgcgatgc
    15481 gataagcttg taaggcatgg aaacctttta aataaagcag tggtgtagag tatttatcaa
    15541 cggcaggatc gcgtaaacgc acagccgaaa tatcataagc tgccgattca atcatagaat
    15601 tatcactacg atatgcttct tcaacaatct cccgaacagc aatcgcgggc attgtttgag
    15661 ttgctagctt atttgccaac atataactta aggcactacc taagttttca tgtttgagta
    15721 atgtcgcatg gaaaaaactc gccaaaattg gctcgcaatc tgccaagcat cttgcttcat
    15781 ttttaatatg atcccagact ctttttagct cttctagcga catactcact ctcattcttt
    15841 ttacattgaa gcctaagtaa atacttaggc ttcttatgtt taaaacagaa ataaataact
    15901 atctaaaata gacttagaac agattgctgt ctatctcatc tttagtagct cggcctaata
    15961 aagataatgc cgcttctttc gcgtctttat gctgatataa aatttggtag atctgctcag
    16021 taataggcat ttcaacacca acttgttcgg ctaatgcgcg aacttcttta gtattacgat
    16081 agccttcaac tacttgtcca attttttctt gggcagtctc cacatcaagg ccttgaccta
    16141 acatcatacc aaagcgacga ttacgagatt ggttatcggt acaggttaaa actaaatcac
    16201 ccaaccccgc catgcccata aaggttgcag catctgcacc taacgcttta cctaaacggc
    16261 tcatttcagc aaggccacga gtaatcaatg ctgtacgcgc attagcacca aaccccatac
    16321 catcagacat acctgcgcca atagcaatca cgttttttac cgcaccacct aattggacac
    16381 cgataaagtc aggattttta tatactcgga aacttttgcc acaatggaac aactgttgta
    16441 actcttcaga gaaggtattg tccgttgaag ccacagaaat cgcagtaggc agacctgctg
    16501 caagctcttt ggcaaaagta gggccagata aaactgcaag cgggatttca ttacctaata
    16561 cttcacgcgc tacatcttgc aataagcgac cagtatgagc ctcaagtcct tttgtcgccc
    16621 aaacaacacg tgcatcctga cgtaaaaagg gtttgatctg ctgtaataca tcaccaaaaa
    16681 catggcttgg gatcacaacc agaatattac ggctagcttc aatggctttt tgcaaagaag
    16741 cttccatgta taaactatca ggaaaggaaa catcgggcag aaaggcctga ttacagcgag
    16801 cttgctctaa cgcagcaaca tgttcaggat catggcccca aagtacaaca tcatggccat
    16861 tgcgcgctaa ggtaatcgct aaagcggtgc cgtatgaacc ggcaccgata actgtcatag
    16921 aagcgttcat taagcttctt cagccccttc attagcggca tcagtttgtt gctgttgtaa
    16981 atagttcata aacaacgcat caaagttaac tggtgctaag ttcagttgtg ggaaagtacc
    17041 acggctgact aagttagtga tacattcacg agcataaggg aacagaacgt tagggcaata
    17101 tgcacctaag caatgtgcta attgagtgcc ttcaatacct tcaacggtga agatacctgc
    17161 ttgttgcact tcacacagga atgcagtttc gttatccata gttgcagtta cagtaacgcg
    17221 cagaataact tcatagacgt tttcagctaa agtgttagaa gaagtatcta aatctaattt
    17281 aacttctggt tgccactctt tttggaaaac ttgtggggca ttaggcgctt caaaagagat
    17341 atcttttgta tagatacgtt gaatttgaaa agtcatctct tggttttgct gttctgacat
//
