LOCUS       KY710730               16125 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2663 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710730
VERSION     KY710730.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16125)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16125)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16125
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2663"
                     /serotype="O65"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16125
                     /note="O antigen gene cluster"
     CDS             2349..3482
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="hypothetical protein"
                     /protein_id="AXY99999.1"
                     /translation="MKNIASKKVGIITMHKVINYGSFLQAYATQYIVEKLGYECEIID
                     YRFPNEWHFKRGVTGTSRVKKYISNIIHKLGIKSGHRKKIKLNKAIDKYLNLSNPYNS
                     PSEIKKKPPIYDIYITGSDQTWNPKHTKGDDTFLLAFAPDKAKKISFSASIAGNNLKD
                     EYKSSFKKLLIRYNNISIRDSGGNNIMKDLIGREVDVTLDPTLMINKDEWSLFAKNRE
                     SIYSNNSYIIFYLITHSFDVTPYIYELLKKLQEKTGLKVYSFSDIPSSFGINYENCAD
                     IGAEDFIELFEKSSYVVTSSFHGTAFAVNFGIPLYSVIDSLDIADDRQISLLNKLEIE
                     QCLVPMNKPFDEIQPIYDIEKEQLNLDKLRADSLKYLRNALSN"
     CDS             3607..5142
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00001.1"
                     /translation="MKNTKSEDVKILLNSIISYTRIFCTTVFSLLTIRFLIEALGVEQ
                     YGIYALVTGIVTIFAFLNSAMTVSTQRYLSFHQGIGNIETLSKIFKSSLLLHVIIGLI
                     LILILLGSIHPLINNFLNINEDLVSRTYYLYSGVVFSIFFTIITVPFNALLVSHENFR
                     VDAIILVFKSFLLLIFSGSLHFLAEEYRLITFSIVIASINIIIFLCYLIYCRKKYSEC
                     NIKANIERKLLKEMTSFALWSLYTNLCYVLNTQGINIVINKFFGAKMNAAYGIAFQIN
                     GQVKNLSQTLLSAMTPQIMKSEGKSNRERAISVSVAASKIGFFLVSLVTIPCLYILPD
                     LIELWLGVIPQYVILFTIFFLFANLINQLTVGITPAIQAVGKIKKFQMVIGTTALVVL
                     PASYFLLDYGISISYVLYLLILIEIITGAMKIWFFSNIFSIRKSWYIKNIVARMVFPF
                     AITNALLYEVIDYFMIKGIIPIIFFSSFAYIIICYLFSLNNEERKEVNSIFLIIAKKS
                     IRK"
     CDS             5147..6142
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00002.1"
                     /translation="MNNIKLVSIIIPMYNVEPFIAKCVNSVIKQTYKNIEIILIDDGS
                     PDLSGDIAEEIALTDKRVKVIRTENRGVSSARNLGIKLSKGEYLVFVDSDDYLSPNYV
                     DYMMKLALDSGAEFIMSKNCHLFPQECVKSDELVDNVEIWSPEKAASELLYPGKIEIG
                     CWNKLFLKEFIINNDIRFPETFYMGEGLNFIVNAAQKANKICVGNRKVYNYRKDNQQS
                     ATTVVNIPKYINALRALDDIDDNKIIKSNRFNSSMIYHKYMTTYIALHTIYITGEYKK
                     YNQEVKKYISFLRSNYFKFINGSFQFMLKLKITSFLINPSLTVKLITLVKKFARL"
     CDS             6577..7758
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00003.1"
                     /translation="MRKFSFFILLFFVPLMNSGLHMFGIVICWFSFCLILSYKEIIHT
                     KLYIVDCFFIMIFFVYCLSTIVLKSYDLNSLKTNTYIVVIIYISIVYIFVSLLSNFNY
                     KYIVKIFRYCLIILAITILFQWSFFFLTGDYIDLNKIVSFGASESRYTSPTFKVMGLI
                     RPTAFFTEPSNASAIISMITFCYMFLIKKLDGYVILGFFISILTLSTAGVLIGSISLC
                     ILLLFYRNNSKTLLFKILFFIFCSIFIFYMLSFSYERISNSSEYDMIATRTVVTNIIQ
                     SQSWYNHLIGNGINILSKPIYTEGNIIYDYSFRDSGFFVNLYYSFGIIGLFSFLFWAR
                     IQIKNNIHLIIFFIILQSKFDYLQPVFWLLIFTISILNSNKMKNKHSSDKNLNTNLLH
                     F"
     CDS             7778..8518
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00004.1"
                     /translation="MSILNMFNLGLENIKERLKFDRQVGYFQKNLILNRKNLPTLSAV
                     FRIKNAEVYLETSVMSIAPICKEIIIVDNNSSDKSLDICYKLQDQLKEICNVKVYSYK
                     HELAIAGENYYKNVTESKSLANYYTFAFSKATSDYVMKADAHLLYTPLALKKIQSKLK
                     KKIRVIIFKGIELYGMKLSFERYIFKNDSSFRFVDGKMYEELQFDYKLSKWEQFRSTI
                     ITPCFIHFKRIRYINNLDSTNIVEKIYK"
     CDS             8565..9383
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00005.1"
                     /translation="MLNIVIFNWNYSKFLKKLLSEIQAIINNPEIRILICDDGSTDCS
                     LDIVRSFISKFNTNNIFVFESKNPNLGRKKPYQGQLESLNKVMDSKFFSTFDHYWLMD
                     ADDYYYFSTLNPKFLDTIKEKKICFTKIKNITNDGIESDMIIKRPIFENNALFPSISV
                     TSSIIVSGEFLLSNKKYIFDDRFDDVWLDSRLNMLACLLKKGEVEYIDKHIFRLIHNS
                     NDSSKMPFSRILKKQISAHDYFRFIHNNKFMLNPRMCVLTILSKCFHAYNRLSK"
     CDS             9504..10235
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00006.1"
                     /translation="MNEIFSVIMPVFNSEKTVSDSINSILAQTYSDFKLYIINDSSTD
                     TSKDIILSFNDERIVYLETSENSGVAYARNIGIMNCTGIYISFLDSDDIWLPNKLECQ
                     LAKLQTGYDVVCSNYFTFNNESDFTLRKSPTIITYSSMLKSNFIGNLTGTYNVNSIGK
                     IFQKKIGHEDYVMWLEILQKSGKAFCIQEPLAKYRISNNSLSSNKLKAIKWQWSIYRN
                     ELNLSIPKSLYYFSHYLLNALKKRR"
     CDS             10239..11186
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXZ00007.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSEKYPEKWIFG
                     DVTKPETLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWYKKDNTNTLIVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATKIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHTHIKVPYAIGLLGGYCFDVLSKVTGKEFPVSSIR
                     IKKFCARTQFKSNSITNTDFKAPVTLQQGIANTVKNEFL"
     CDS             11418..12584
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00008.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             12596..13099
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00009.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13170..13727)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXZ00010.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRIYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIKQSSM"
     CDS             complement(13727..14560)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00011.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14639..15655)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00012.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(15655..16125)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5626 a   2664 c   2530 g   5305 t
ORIGIN
        1 atgctagaac aacatatcga agctgaatta gctcaagatc cggctaacga tctcctttgg
       61 tggcgtcgat taattcgagc tattgataaa tgggctcccc ctggccaacg tttaattatt
      121 gtcacgagtg aaggccggat tatcggtgct caacgtaatg agatgcaagt tgtcagaaac
      181 tttattggtc aatctgataa cgccgatcac cccaaaaaga aaaaatatgg tcgctctgag
      241 atgctagggc ctttttctgt aagggatggt gaagatcatt accaacttta tctcgtgcgc
      301 ccttcaagta gccctcaatc tgactttatc aacttattat ttgataaacc attgttgtta
      361 ctcatattca ccatgcttat cagcacacca ttattagtat ggctttcttg gagcttagca
      421 aaacccgcaa gacagcttaa aaatgccgct gacgatgtgg ctaaaggtaa cctacgccct
      481 caccctgaac tagaaactgg ccctcaagag tttttagccg caggcacgag ttttaatcag
      541 atgataagtg ctcttgaacg tatggtagag gcgcaacaac gtctgatctc tgatatttct
      601 catgaactac gtaccccatt aacacgctta caattagcca gtgcattgtt acgtcgtcgt
      661 agtggagaga gcaaggagct agagcgtatt gaaactgaga ctcaacgcct tgatggtatg
      721 attaatgact tactagtact ttcccgtaat cagtataaaa acgagttatt acgcgaaacg
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataacg ctaaatttga ggccgaacaa
      841 agtaataaga cactgcaagt cacaacacct ccaggagcct ggcctattta ctgcaatcca
      901 tattctctgg cgagtgcatt tgagaatata gtccgtaatg ctctgcgtta ttcaaattct
      961 cgcattgaag tcgcgtttac tgaacaaaac caaggtatca ccattattgt tgatgatgat
     1021 ggccctggcg ttagccctga agatagagag catatcttcc gaccattcta tcgcactgac
     1081 gaagctagag atcgtgaatc cggtggtact ggcttaggat tagcgattgt tgaaactgcc
     1141 atcagccaac acagaggcca tgttaaagcc gatgacagtc cattaggtgg gttgagggtt
     1201 gagatttggt tgccgagagc gggtgcaagt aacaaatcgc caacggctta atagagtaat
     1261 taaactgttg tatcaaaata tcctgccatc tattgaagaa taggtggcat attctttttg
     1321 ttcgcattta ttgatgcaat tcagaatgat ctggctgaat cgatcgctat tccgatattt
     1381 atatatctac aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt
     1441 ctactctcag tgcggatttt cgcatattgt taaagtaaat ttaaagaacc aatagtaaaa
     1501 aacatccttg aagactgaaa ccactaaaaa tattgattta aattgataac agagtctata
     1561 ccaagactta gttttagaag acagattaca acatctacaa tgatggaata ttttagatat
     1621 gatgacatat tatattaagt atcaatacct aggccatcag acctacatac tttcacaact
     1681 aaattattat aaatgctcac aattatccaa aatacaacta gccaaaaaaa accaaaaaat
     1741 agtctaatta aagatcctac accttcaagg ttacaataaa gataaaggct attctcaaaa
     1801 ctattctttt gatataattt atcaatatat ttgaatggat tataggtaaa aatatcagta
     1861 aactggtagc tgtaagcctg gggcggtagc atgcccataa acgattataa ctcaatccca
     1921 ccgtattgta acagccgatt tatttgtaga ctaattaagg ttatattctc ttgtggttct
     1981 aaaagtacaa cttgacaata ctaaaaaata aaacaccttt tattcccaac atagataaca
     2041 aatgtattcc cccaacccga attactaata atcttaagat atgaaacatt tatttctgat
     2101 taatctaata tgaatttatt taaaaagatt tatattaata aaggtgtaaa atgtgttttt
     2161 atttataact acgctctgaa tgatgggtga ttgaaatatt tatctgtatg taaaaagcca
     2221 gattcaattt tttagctaac aaggctacta cattaaagat gtttattact ctagaaaatt
     2281 aagactgtaa catataaagt tatatagtat atttacactt tctcattgtc ccaataaagt
     2341 aaaattaaat gaaaaatata gcatcaaaaa aagttggtat aattaccatg cataaagtaa
     2401 ttaactatgg atcattctta caagcatatg ctactcaata tatcgtcgag aaattagggt
     2461 atgaatgtga aattattgat taccgattcc caaatgaatg gcattttaaa agaggtgtca
     2521 caggaacaag tagagtcaaa aaatatatat caaatataat tcataagctt ggaataaaat
     2581 caggacatag aaaaaaaata aagttaaata aagctattga taaatacttg aatttatcaa
     2641 acccttacaa tagtccatct gaaattaaaa aaaaaccacc tatttatgat atttatatca
     2701 caggaagcga tcaaacatgg aaccctaagc atacaaaagg agatgatact tttttattgg
     2761 catttgcccc tgataaagct aaaaagatct cattctcagc tagtatcgca ggtaataatt
     2821 taaaggacga atataaatct tcatttaaaa aactcttaat tagatataat aatatctcaa
     2881 taagagattc tggtggtaac aatataatga aagatcttat tggaagagaa gttgatgtaa
     2941 cattagatcc aacattaatg ataaacaaag atgaatggtc attatttgct aagaatagag
     3001 agtcaatata ctccaataac agttatatta tattctatct aattactcac tcttttgatg
     3061 taacgcctta tatttatgaa ctcctaaaaa aacttcaaga aaaaacaggg ttaaaagtct
     3121 attcttttag tgatatacca agctcatttg gtattaatta tgaaaactgt gctgacatcg
     3181 gtgctgaaga ttttattgaa ttatttgaaa aatcatcgta tgtagtaaca tcatctttcc
     3241 atggtacagc ttttgccgtc aacttcggta ttccattata ttctgtaatt gatagcttag
     3301 atatagctga tgatcgacaa atatcactat tgaataaatt ggaaatcgaa caatgcctag
     3361 ttcccatgaa taaacctttt gatgaaatac agcctattta tgatatagaa aaagaacaac
     3421 tcaaccttga taaattaaga gcagattcat taaagtatct acgcaacgct ttgtctaact
     3481 agaattcatc ttaaatataa gaatttataa ccactggtta cgttttaaat taaagagtta
     3541 tctttaattt ctaatgtatt ataatttaaa gttgcatttt ggtttataaa aaatctaggc
     3601 atcataatga aaaatactaa gagtgaagat gtcaaaattc tattaaactc aataatttct
     3661 tatacacgca tattctgtac aacagttttc tcgcttctaa caataagatt tttaatagaa
     3721 gcactgggag tggaacaata tggtatatat gcattagtta ctggaattgt tacgatattt
     3781 gcttttttaa actcagcgat gacagtatct acacaacgat acttatcctt tcatcaaggc
     3841 atcggtaata tcgagacctt aagtaagata tttaaaagta gtttattatt acatgttatt
     3901 attggtttaa ttctaatact catattactt ggctcaatcc atccacttat aaataatttt
     3961 ttaaatatca atgaagattt agtaagtcgt acctactatt tatacagcgg tgttgtattt
     4021 tcaatatttt tcacaattat aactgttcct tttaatgctt tgcttgtttc acatgaaaac
     4081 tttagagtcg atgctattat attggttttt aagtcatttt tattgctgat attttctgga
     4141 agtctccatt ttttagcaga agagtaccga ctaatcacat tttctattgt tatagcaagt
     4201 ataaatatta ttatattctt atgttatcta atatattgta ggaaaaaata cagtgaatgt
     4261 aatataaaag ccaatataga aagaaaacta ttaaaagaaa tgacttcttt tgcgttatgg
     4321 agtttataca ctaatttatg ttatgttcta aatactcaag gaataaatat agttattaat
     4381 aagttttttg gtgccaaaat gaatgcagcc tatggaattg cttttcaaat aaatggacaa
     4441 gtaaaaaatc tatctcaaac attactttct gctatgacac ctcaaataat gaaaagtgaa
     4501 ggaaaatcta atagggaaag agcaattagt gtttcggtag ctgctagtaa aattggtttt
     4561 tttttagttt ctttagttac tataccatgt ttatatattc ttcctgattt aatagaatta
     4621 tggctaggtg ttattcctca atatgtaatc ttatttacca tatttttctt gttcgctaat
     4681 ttaataaatc aattaacagt gggaatcacc cctgcaatac aagctgttgg taagataaag
     4741 aaatttcaaa tggtaattgg aacaacggct ctagttgttc ttcctgcatc atatttttta
     4801 cttgattatg gcatttcgat atcttatgta ctctatcttc taatccttat tgagataatt
     4861 acaggagcaa tgaaaatttg gttcttcagt aacatctttt ctatcaggaa atcatggtat
     4921 ataaaaaata ttgtagctag aatggttttt cctttcgcaa taactaatgc tttactatat
     4981 gaagttatag actattttat gataaaagga attataccta taatattctt tagttctttt
     5041 gcatatatta taatatgcta tctctttagt ttaaataatg aagaaagaaa agaagtgaat
     5101 tcgatatttt taattatagc taaaaaatca ataagaaaat aataccatga ataatattaa
     5161 attagtctct atcataattc caatgtataa cgttgaaccg tttatagcta agtgtgtaaa
     5221 ctccgtaata aaacaaacat acaaaaatat tgaaataatt ttaattgatg atggctcacc
     5281 tgatttatca ggagatattg ctgaagaaat tgccttaacc gataaacgag ttaaagttat
     5341 tcgaacagaa aatcggggtg ttagtagtgc taggaatcta ggaataaaac tatctaaagg
     5401 cgaatatttg gtctttgtag atagcgatga ctatttatct ccaaattatg ttgattatat
     5461 gatgaagtta gctcttgata gcggagctga gtttatcatg tcaaagaatt gccacttatt
     5521 ccctcaagag tgtgtaaaat ctgatgaact tgtggataat gtggaaattt ggagccctga
     5581 aaaagcggct tctgaattac tttatccagg taaaattgaa attggttgtt ggaataaact
     5641 atttttaaaa gaattcatca ttaataatga tatacgattt cctgagactt tttatatggg
     5701 tgaaggactt aactttattg ttaatgccgc tcagaaggct aataaaatat gcgttggaaa
     5761 tagaaaagta tataactacc gaaaagataa tcaacaaagt gccactactg ttgtaaacat
     5821 acctaaatat attaatgcat tacgcgcttt agatgacatc gatgataata aaattataaa
     5881 atcaaatcgc tttaattcat caatgatcta tcataaatat atgacaacat atatagcact
     5941 acatactata tatatcacag gtgaatacaa aaaatataat caagaagtta aaaaatatat
     6001 atcttttctt aggagtaatt attttaaatt tataaatggt agtttccaat ttatgcttaa
     6061 attaaagatc acctcctttt tgattaaccc tagccttaca gttaaattaa ttactttagt
     6121 aaaaaaattc gcacgtctat aaaaaataaa aatataattt ataagaattc tcaatttagg
     6181 aaatatacta aattccgtac ctagtattaa ctttgtataa aaagtaatat catattttaa
     6241 tgaataatat tcatgctctc ttatgatgaa tgttcaaggt ggtaagttat aacatataaa
     6301 tatggtaaca cagcctacaa agttacattt tctctgtact aatttagtgt acattttagt
     6361 atcattgaat tttcctatat aatcttactt ttctaataaa attcctaaca taattaggat
     6421 tgaattttat gaagatttga aatcaataat attggttagt ttaatttaga agttgctatt
     6481 aataataaat gattcatagc atttcttata acttaagtgg tttcaacaag aatattacaa
     6541 aagtataatt taattattat ctattttaga gctcatatga gaaaattttc tttttttata
     6601 ctactttttt ttgtgccatt aatgaactcc ggcttacaca tgttcggaat agttatttgc
     6661 tggttttcat tctgtctaat tttatcatac aaagaaatta tacatactaa attgtatata
     6721 gtagattgct tttttattat gatttttttt gtatattgtc tcagtaccat agttttaaaa
     6781 tcatacgatc ttaactctct taaaacaaat acatatattg tagttattat atatatatca
     6841 atagtatata tatttgtatc attattaagc aactttaatt ataaatatat agtaaaaata
     6901 tttcgatatt gcttaattat attagctatt actattttat ttcagtggtc attctttttt
     6961 ttaactggtg actatattga tctcaataaa attgtttcat ttggagcatc tgaatcaaga
     7021 tatacaagtc ctaccttcaa agttatggga ctaattagac ctacagcttt ctttactgaa
     7081 ccttcaaatg caagcgctat aatatccatg ataacctttt gctatatgtt tttgataaaa
     7141 aaacttgatg gttatgtaat attagggttc tttatttcca tattaacatt atcaacagct
     7201 ggtgttttaa ttggctcgat ttcattatgt atattgcttc ttttctacag aaacaattct
     7261 aaaacacttt tattcaaaat attattcttt atcttctgct caatttttat tttttatatg
     7321 ctttcattta gttatgagag gattagtaat tcctccgaat atgatatgat tgcaactaga
     7381 actgttgtaa ccaatattat acaaagtcaa tcttggtata atcatttaat aggtaacggt
     7441 ataaacatac tatcaaaacc tatatacaca gaaggtaata ttatctacga ttacagcttt
     7501 agagatagcg gattttttgt taatctttat tattcatttg gtattatagg actattttca
     7561 tttctatttt gggcaaggat ccagattaaa aataatatac atttaataat tttctttatt
     7621 atattacaaa gtaagttcga ttacttacaa cctgtttttt ggttgctaat atttactatt
     7681 tcaatactga atagtaataa aatgaaaaat aaacattcta gtgataaaaa tttaaatact
     7741 aatttattgc atttttaata tattaaaagg tttttttatg tctattttaa atatgttcaa
     7801 tttaggactt gaaaatataa aagaaagatt aaaatttgat cgtcaggttg gctattttca
     7861 aaaaaatcta atattaaata gaaaaaattt accaacatta tctgcggtct ttagaattaa
     7921 aaatgcagaa gtatatttag agacttccgt tatgtctatt gcaccaatat gtaaagaaat
     7981 tattatcgta gacaacaatt catcagataa atctttagat atatgctata aactacaaga
     8041 tcaacttaaa gaaatatgca atgtaaaagt ctactcatat aaacatgaat tagccattgc
     8101 tggtgaaaac tattataaaa atgtaacaga atctaaatcg ttagcaaatt attacacctt
     8161 cgcattttct aaagcaacat cagattatgt aatgaaagcc gatgctcatt tattatacac
     8221 tcctttagcc cttaaaaaga tccaaagtaa acttaaaaag aaaataagag ttataatatt
     8281 taaaggtatc gaactctatg gtatgaagtt atcttttgaa aggtatattt tcaaaaatga
     8341 ttcatctttt agattcgtgg acggaaaaat gtatgaagaa ttacagtttg attataagct
     8401 ttcgaagtgg gaacagttta gatcaaccat cattacgcct tgttttatac atttcaaaag
     8461 aattcgctat attaacaatc tagatagtac taatatagtt gaaaaaatat ataagtaaca
     8521 tgttttataa taaaatctgt ataaatttaa ataggggatg aagattgtta aatatagtta
     8581 tttttaactg gaattactct aaatttctaa agaaattatt aagcgaaata caagctataa
     8641 tcaataatcc agaaattaga attctcattt gcgatgatgg atctactgac tgtagtctag
     8701 atatcgtgag aagttttata tccaaattta acacaaataa tatatttgtt tttgaaagta
     8761 aaaatccaaa tttgggtcga aaaaaaccat atcaaggcca gcttgaaagc ttaaataaag
     8821 taatggatag taaatttttt tctacatttg atcattattg gttaatggat gctgatgatt
     8881 actattattt ttctacttta aatcctaagt ttttagatac aataaaagaa aaaaaaatat
     8941 gttttacaaa aataaaaaat atcacaaatg atggcataga gagtgatatg atcataaaga
     9001 ggccaatctt tgagaataac gctttgtttc cttcaatatc tgtgacttcc tcaattattg
     9061 tctcaggaga gttcttactt tccaataaaa aatatatctt tgatgatagg tttgatgatg
     9121 tatggttgga ctctcggtta aatatgttag catgtttatt aaaaaaagga gaggttgaat
     9181 atattgataa acatattttt aggttaatac ataattctaa tgattcaagt aaaatgccat
     9241 tttctagaat attgaaaaag caaattagtg ctcatgacta ttttagattt attcataata
     9301 ataagtttat gttaaatcca agaatgtgtg ttttaactat actatcaaag tgctttcatg
     9361 catataatcg tctatcaaaa taaataacca actaatgata agaataagat tcaccttatt
     9421 cattcgaaaa atcaataact cgaacatcat ttatcaagaa acaaatctaa aaatattaac
     9481 atctcagaaa atttgagaaa aaaatgaatg aaattttttc agttataatg cccgtattta
     9541 attcagaaaa gaccgtatct gatagtatca actcaatact cgctcaaaca tactccgatt
     9601 ttaaattata tattatcaat gattcatcca cagatacttc taaggatata atcctatcct
     9661 ttaatgatga acgaatagtt tatttagaaa catctgaaaa ttcaggggtc gcttatgcta
     9721 gaaatattgg aataatgaat tgtactggaa tatatatatc ttttctagat agtgatgaca
     9781 tatggctacc taataaatta gaatgtcaat tagctaaatt acaaactgga tacgatgttg
     9841 tttgctctaa ttattttact tttaataatg aatccgattt tactctaaga aaatctccaa
     9901 ctataattac atattcatca atgcttaaat ctaatttcat tggcaattta acaggtactt
     9961 ataatgtaaa tagcattggg aaaatttttc agaaaaaaat tggtcatgaa gattatgtta
    10021 tgtggttaga aatattacaa aaatctggca aagcattttg tattcaagag cctctagcta
    10081 aatataggat ttcaaataac tcactatcct caaataaact taaggccata aaatggcaat
    10141 ggtcaattta tagaaatgaa ctaaatttat cgattcctaa atcgctatac tacttttcac
    10201 attacttact aaatgcattg aaaaaaagaa gataaactat gttaacaatc atcggtggtt
    10261 caggttttat tggcacacgc ctttcagatc aactaactaa agaaaatatt gaatttaaaa
    10321 ttgtagatat tgctaaaagt gaaaaatatc ctgaaaaatg gatatttggt gatgtaacaa
    10381 aaccagaaac attacttgaa ccattaaaag gctctgatgt tattatcaac ttagcggcac
    10441 aacataaaga taatgttcat cctattagtt tatattatga agttaatgta gatggagcta
    10501 aaaatgtttg tgatgttgct gaacaactaa atattaaaca tatcgtattt acttcatctg
    10561 ttgctgtata tggatttgtt gaaaaagaaa cgggtgaaga tggtgagttt catccattta
    10621 atgattatgg aaaatcaaaa cttgaagctg aacataagta tgaagcttgg tataaaaaag
    10681 ataatacaaa tacattaatc gttatcaggc caaccgttgt atttggtgaa ggcaatagag
    10741 ggaacgtata taacctattc cgccaaatag catcgggtaa atttttaatg gtgggttcag
    10801 gtaataataa aaaatctatg gcttatgtag aaaacatcgc tgctttctta aaatttgcta
    10861 ctaaaataga atctggtcat catatattta attatattga taaacctgat tttactatga
    10921 atgaattgac agatatcatt tatacagctc tagataaacg acatacccac attaaagttc
    10981 cttatgctat tggattactt ggtggatatt gttttgatgt tctttctaaa gttacaggta
    11041 aagaattccc tgtaagcagt attcgaatca aaaaattctg tgcccgcact caatttaaat
    11101 caaatagtat tactaatact gattttaaag cgccagttac tcttcaacaa ggtattgcta
    11161 acacagttaa aaatgaattt ttataataga ttaattttaa atagtctttg tattaacaaa
    11221 taattaaaat acaccctaca cttctctact tattttctaa taaaaaatta attttacatt
    11281 aatttaataa gtatatgatc cttatgattg ctaaatatac tataccttta ctaaagagtg
    11341 agaattaaca ctaaaatatc tatttttatt ctagtatagt gaaattataa atacatatac
    11401 tttagataag agaaaccatg aaaatcacca tctccggtac cggctatgtc ggtctatcta
    11461 acgggatact tctttcccaa cacaacaaag ttgttgcgtt agatatcaat aaagaaaaag
    11521 ttgaattact taacaaaaaa atctcgccaa ttacagacaa agaaattgaa gagtttttag
    11581 caaccaaaga gctcgacttt acagcaacat tagataaaga attcgcttat aaagatgctg
    11641 aattcgttat cattgccaca ccaaccgatt atgatcctaa aacgaactat ttcaacacct
    11701 cttctgtaga gtctgttatt cgtgatgttt tagaatataa tccaaacacg acaatgatta
    11761 taaaatcaac cattcctgtt ggttttacca aagagatgag agagaaatat aataccaata
    11821 atattatttt ctcccctgag ttcttacgtg aaggtcgtgc gctttacgat aacttatacc
    11881 catctcgtat tgtgattggt gaacaatcag aaagagcaca aaagtttgct gaccttttac
    11941 aacaaggcgc actgaaaaaa gatattgatg ttctgttcac tgactccaca gaagccgaag
    12001 cgattaaatt atttgctaat acttatctag cactccgtgt tgcttacttt aatgagctag
    12061 atagctatgc cgaatcttat ggcctaaatg caagacaaat cattgaaggt gtctgcttag
    12121 atccgcgtat tggtaatcac tataataatc catcattcgg ttacggcggt tactgcttac
    12181 caaaagatac caagcaactg ttagccaact acgactctgt ccctaataat attattagtg
    12241 ccatcgtgga agcaaaccgc acacgtaaag attttattgc agactctatt attgcaaaat
    12301 cacccaaaat cgtcggcgta tatcgtttaa ttatgaaatc tggctcggat aatttccgtt
    12361 cttcttctat ccaaggcatt atgaaacgca ttaaagccaa agggattgaa gtggtgattt
    12421 atgaaccaga gatgaaagaa gagaccttct ttaattcaaa attaattcgt gatttaaatg
    12481 agtttaaagc aatgtctgat gtcattatta ccaatagaat gtcatcagca ctaaacgatg
    12541 ttgaagaaaa agtttatact cgtgacctct ttggtaacga ttaaatcgat tttatatgct
    12601 caatatcgtc ttatttgaac ccgaaattcc accgaatacg ggtaatatta tccgcctgtg
    12661 tgctaacaca ggctttcatc ttcatttgat ccaaccattg ggttttactt gggatgataa
    12721 acgtctacgt cgtgcagggc ttgattatcg tgaatttgct gatattaaac agcaccacga
    12781 ttattatgct tttttagaaa gtgaaggttt aaaccctgat aatgcacaat caccatcagg
    12841 tgcgcgtgta tttgccttaa ccacaaaagg cacgccagca catagcaatg tcagttatca
    12901 agatggagat tatctcctgt ttggccctga aacccgtggt ttgccacctt atgtattaga
    12961 taatatgcca acacagcaga aaatcagagt cccaatgctt gctgatagcc gcagtatgaa
    13021 tctgtcaaat acggttgcgg ttgtggtttt tgaggcatgg cgccaacttg gttatcctgg
    13081 tgcattacta agagactgaa atgcaaaaag cgctgatcat aaataatgac tagcgctttt
    13141 taacttcaaa taactaacat gttaatttat tacattgagg attgttttat ttcagcaaca
    13201 accttcgtga tatatcgaca cccttcccct acatccttca gtactaatgc atgagctcca
    13261 atcaccgcat tatcaccaat ctctatctca ccgccgataa tcgatgagtt acaaccaata
    13321 taaacgttat taccaacccg aatatccgct ttgccattct tttcttttac accgattgtc
    13381 acaccttgca ttaacgtcac attctcgcct agctgacagt aattagttag tacgatccct
    13441 atgggatgtg gtaatgaaag gcctttacct atgcgcgttc ttagtgagat atcgttgcaa
    13501 tatatacgat tgtttttagc accaatacga atgcctaatt ttttgataaa acgattttta
    13561 gaaaaataaa agaattgggc gacacgccac caaaatagat aacgtgaacg ttggcagaaa
    13621 aaaacacggt gataaagtct tatccaacta aaaggcttgt tatcacctat atactcagcg
    13681 tgtaaacaag cctttagttc agaaagggtg acttcagtta atgccattaa atgccatcac
    13741 cacattcaaa accgggtacg acaccattaa agtgttgatc catatcgagt gaaggcttgt
    13801 cactggtcgg cttaccaaca atgcgagcag gaacaccagc aacggtagtg tgcgcaggaa
    13861 cttcatgtaa aacgactgat cccgcaccaa ttttggcacc gcgaccaatc tcgatattgc
    13921 cgagtatttt tgcacccgca ccaatcatta caccttcacg cactttagga tgacggtcac
    13981 cacaagtttt acctgttcca cctaacgtca cagattgtaa aatagaaaca tcgttctcta
    14041 cgatagcggt ttcaccaatt acaatgcctg ttgcgtgatc aagcataata ccgtggccaa
    14101 tacgggcagc tggatgaata tcaacaccaa aggtcacaga aatctggttt tgcagataca
    14161 ccgctaacgc tttgcgatct tgattccata accaatgcgc gatgcgataa gcttgtaagg
    14221 catggaaacc ttttaaataa agcagtggtg tagagtattt atcaacggca ggatcgcgta
    14281 aacgcacagc cgaaatatca taagctgccg attcaatcat agaattatca ctacgatatg
    14341 cttcttcaac aatctcccga acagcaatcg cgggcattgt ttgagttgct agcttatttg
    14401 ccaacatata acttaaggca ctacctaagt tttcatgttt gagtaatgtc gcatggaaaa
    14461 aactcgccaa aattggctcg caatctgcca agcatcttgc ttcattttta atatgatccc
    14521 agactctttt tagctcttct agcgacatac tcactctcat tctttttaca tcgaagccta
    14581 agtaaagact taggcttctt atgtttaaaa ctgaaataaa taactatcta aaatagactt
    14641 agaacagatt gctgtctatc tcatctttag tagctcggcc taataaagat aatgccgctt
    14701 ctttcgcgtc tttatgctga tataaaattt ggtagatctg ctcagtaata ggcatttcaa
    14761 caccaacttg ttcggctaat gcgcgaactt ctttagtatt acgatagcct tcaactactt
    14821 gtccaatttt ttcttgggca gtctccacat caaggccttg acctaacatc ataccaaagc
    14881 gacgattacg agattggtta tcggtacagg ttaaaactaa atcacccaac cccgccatgc
    14941 ccataaaggt tgcagcatct gcacctaacg ctttacctaa acggctcatt tcagcaaggc
    15001 cacgagtaat caatgctgta cgcgcattag caccaaaccc cataccatca gacatacctg
    15061 cgccaatagc aatcacgttt tttaccgcac cacctaattg gacaccgata aagtcaggat
    15121 ttttatatac tcggaaactt ttgccacaat ggaacaactg ttgtaactct tcagagaagg
    15181 tattgtccgt tgaagccaca gaaatcgcag taggcagacc tgctgcaagc tctttggcaa
    15241 aagtagggcc agataaaact gcaagcggga tttcattacc taatacttca cgcgctacat
    15301 cttgcaataa gcgaccagta tgagcctcaa gtccttttgt cgcccaaaca acacgtgcat
    15361 cctgacgtaa aaagggtttg atctgctgta atacatcacc aaaaacatgg cttgggatca
    15421 caaccagaat attacggcta gcttcaatgg ctttttgcaa agaagcttcc atgtataaac
    15481 tatcaggaaa ggaaacatcg ggcagaaagg cctgattaca gcgagcttgc tctaacgcag
    15541 caacatgttc aggatcatgg ccccaaagta caacatcatg gccattgcgc gctaaggtaa
    15601 tcgctaaagc ggtgccgtat gaaccggcac cgataactgt catagaagcg ttcattaagc
    15661 ttcttcagcc ccttcattag cggcatcagt ttgttgctgt tgtaaatagt tcataaacaa
    15721 cgcatcaaag ttaactggtg ctaagttcag ttgtgggaaa gtaccacggc tgactaagtt
    15781 agtgatacat tcacgagcat aagggaacag aacgttaggg caatatgcac ctaagcaatg
    15841 tgctaattga gtgccttcaa taccttcaac ggtgaagata cctgcttgtt gcacttcaca
    15901 caggaatgca gtttcgttat ccatagttgc agttacagta acgcgcagaa taacttcata
    15961 gacgttttca gctaaagtgt tagaagaagt atctaaatct aatttaactt ctggttgcca
    16021 ctctttttgg aaaacttgtg gggcattagg cgcttcaaaa gagatatctt ttgtatagat
    16081 acgttgaatt tgaaaagtca tctcttggtt ttgctgttct gacat
//
