LOCUS       KY710732               13646 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2667 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710732
VERSION     KY710732.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2667"
                     /serotype="O69"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..13646
                     /note="O antigen gene cluster"
     CDS             1614..2900
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00030.1"
                     /translation="MKLLKGASIYLISNILNSAIPFLLLPILTRYLSTEEYGKIAMFQ
                     IFLAGIGTLIGCNTISAISRKYFDDRKNDNELKEYNNSCLFILFISSVFIFIIVSIFI
                     NQLSNFLSIPSDWIFIAIIISTINYITSLRLIQWQVRGEAKKFGILQLSNSSTNITLS
                     LVLIIIFNQGAQGRIDAQFITSILFFILSAFLLIKGNLISPIPKPTYSLAKDALSYSI
                     PLLPHTIGVFFISFIDRFIINKEMGLGEAGIYMVAVQLSSTIAIIFNSLYQAYIPWLF
                     DRLKKNDENEKIKIVKYTYLFFIFLIFSGLIIFIISPIIVKFLVGEQYHPITNIIGWL
                     CIGQIFGGMYLIMSGYIYYSKKTSRLALITISSGITNVVLLVVFVPMYGLIGAAVSFS
                     FVKIVQFIAVYLSSIKCVNMPWFNFTGNTNEFHKKN"
     CDS             2878..3444
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="acetyltransferase"
                     /protein_id="AXZ00031.1"
                     /translation="MNFIKKIRIVFLIKLISILKLFIKNEKFSNLLNFYNYKLIKERL
                     KNRNVFIGNNTIIYNTEFSYSQKGDHFYIGNNCTITGAYLLGHDASPTLFIDDLIVHK
                     DVWKNGSRRSYRAPIRIGDNVFIGVNAIILPGIHIGDNIVIAAGSIVSCDLESNFVYA
                     GNPAKKIKPIEEYIEKYKNIFYSNPESF"
     CDS             3450..4718
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00032.1"
                     /translation="MNLYSYKNNFNLADFIVLLFFFLAPLSITFFEKKNLAILIFFSI
                     SFIILNIKNIYKIKIEKIAFTFILSYLIYITISSIGMIFNFNKLNYSYLANFFSRLLT
                     ITIGITFLYIITYWSERNSNKLKVIPKVSFYICFVFLLLAFYQIIAFKYHLPFFETRS
                     FIYGSNAALNSSLGFRITSIAREPNYYSPLLFESLIIAWIVLKRKSFLIFSILTIIIM
                     YKTYSTGVYLHAALLLAILFLTKKTSSLFKLTLTIVFLFLISIIIYVGLDSTSLEYFS
                     DKLRNELSGDSTRSYVIITIITGFLGCHFINQLFGNGLNSLDFYNILSTNNVKNIDFS
                     ISNNLVIDFLWDGGIIGVLVFSFGLFYIGKLLFNSFRKNKYFYASFLLFSSFLITSLY
                     RSEYASLHFFWTIANIVILYKLGKENKNEH"
     CDS             4708..5490
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00033.1"
                     /translation="MNIDMLYSIYRDGIVKFLENIPSPKDNIKIIIVHQVLPEDNNDY
                     LLEPLLSSRNDIQYIKSYTKGVTKSRNIALSKAKSDIVLFCDDDVKYVDNLNDIITSA
                     YDKNPKYGFLTFSYLKNNEWHKFKKQNFEHNYRTILNIGTIEVTCLRKHIFDNHFKFP
                     EDLGAGAKYFLCDEPVFISQFLKKKLKGKYIAEVIGEHPDDSSGLIFNNIYAFKSRIL
                     CFQRIFGLLKGTFLYYLFLIKNFKKFNGLKSVINAFILVHSK"
     CDS             5487..6197
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="hypothetical protein"
                     /protein_id="AXZ00034.1"
                     /translation="MNIKFKKFGLTFNDKWNHKDINFFDLFKFTSYKGHNNFSTSLPF
                     LKKQKRSIVNYLSFDEDTLWSNVRKNTRNEINKIKKEDIKYSINNVSLHEFINLYNEF
                     IDFKNLSLTQLSEKRLEKYNNNILLTSTEISGKWQSIHVYLIIDKYSELLYSITNHNA
                     DHKTTGMANRFHHWNDLIHFKNHDYEAYDWGGIAFGDMDGIAKFKLSFGGEEIISNIY
                     FSPLYYLTLLIREKPWKK"
     CDS             6185..6886
                     /note="orf07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf07"
                     /product="hypothetical protein"
                     /protein_id="AXZ00035.1"
                     /translation="MEKIIINADDFGISQNVNKAIVTCFEHEIINSTTILANMYSTNE
                     AIKLAKDLNLSVGIHLNLVEGCSLTSKIKNFSQVYNKENSAFEFNISRSSLKLNNSLL
                     EAIKIELDSQICYLLDNGIDPTHLDSHQHTHTIFPIFNIVKSLGKKYNLKIRIPRTTG
                     SKSQLKIIYKKIIMLNLISNKINLTNEFINIDEFKNGYAHKGNLEVMVHPDINEAGDI
                     FCNTTNTILIKKEDL"
     CDS             6883..8016
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00036.1"
                     /translation="MKALLLNTSFYPNFGGVENSLRSICKVLCESGWDVDIITSDDGY
                     LPSSEIIFGANVYRYKQRKFGLFLFSIKNLLNKNKEKNYDLIISRHTLTSLALLLNNT
                     RNFHFIVPGVHYFQNNKKNSSLIEKVKFISNIIIEKYVFNKVSNIYCFSKTMNEQISF
                     YRKNKEIMQTPPGVDLNRFFPVDKEIKSSLRKKYNLPLNKKIIFCLGRFVKLKNFETI
                     IKTIYFLDDDYHLLLVGNGPLLKNYLELISKLNIEDKVTIIKPTQTPEEFYSTADIFC
                     LPSTYEPFGQVLLEATACHLPIVALDSDFFQTASKEIYQDYNNLITFTDGNSPENFKE
                     KILNLSKKDFDKTQASSFLKKHSWNNLITNIINNSKNHTSKYK"
     CDS             8077..9255
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00037.1"
                     /translation="MLSKMKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNK
                     KISPITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVE
                     SVIRDVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPS
                     RIVIGEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNEL
                     DSYAESYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNI
                     ISAIVEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGI
                     EVVIYEPEMKEETFFNSKLIRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND
                     "
     CDS             9292..10083
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00038.1"
                     /translation="MLSKNTNFLISIIMPSYNSGEYICKSIDSVLRQTYKNFELIIID
                     DCSNDNTIEIVSSYKDERIKVLKNEANQGAGITRNKGLSLANGRFICFLDSDDYWRED
                     KLEKQLNFMLSNKIDFCFSAYDRVSEDNTFICKIGVPEKVSYSDMLKVCSIGCLTVMF
                     DKEKFKDFSMPSIRKRQDYALWLKLLKQTPYAYGINESLGYYNIRENSISSNKLNAAK
                     YQWRIYREEERLNIFHSIYYFSHYAFNGFFRSKFPSLAKKIGVLK"
     CDS             10117..10620
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00039.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(10691..11248)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXZ00040.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRQYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGYRYITKVVAEIKQSLM"
     CDS             complement(11248..12081)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00041.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(12160..13176)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00042.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(13176..13646)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4723 a   2270 c   2146 g   4507 t
ORIGIN
        1 atgctagaac aacatatcga agctgaatta gctcaagatc cggctaacga tctcctttgg
       61 tggcgtcgat taattcgagc tattgataaa tgggctcccc ctggccaacg tttaattatt
      121 gtcacgagtg aaggccggat tatcggtgct caacgtaatg agatgcaagt tgtcagaaac
      181 tttattggtc aatctgataa cgccgatcac cccaaaaaga aaaaatatgg tcgctctgag
      241 atgctagggc ctttttctgt aagggatggt gaagatcatt accaacttta tctcgtgcgc
      301 ccttcaagta gccctcaatc tgactttatc aacttattat ttgataaacc attgttgcta
      361 ctcatattca ccatgcttat cagcacacca ttattagtat ggctttcttg gagcttagca
      421 aaacccgcaa gacagcttaa aaatgcggct gacgatgtgg ctaaaggtaa cctacgccct
      481 caccctgaac tagaaactgg ccctcaagag tttttagccg caggcacgag ttttaatcag
      541 atgataagtg ctcttgaacg tatggtagag gcgcaacaac gtctgatctc tgatatttct
      601 catgaactac gtaccccatt aacacgctta caattagcca gtgcattgtt acgtcgtcgt
      661 agtggagaga gcaaagaact agagcgcatt gaaactgaaa ctcagcgcct tgatggtatg
      721 attaatgact tactagtact ttcccgtaat cagtataaaa acgagttatt acgcgaaacg
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataacg ctaaatttga ggccgaacaa
      841 agtaataaga cactgcaagt cacaacacct ccaggagcct ggcctattta ctgcaatcca
      901 tattctctgg cgagtgcatt tgagaatata gtccgtaatg ctctgcgtta ttcaaattct
      961 cgcattgaag tcgcgtttac tgaacaaaac caaggtatca ccattattgt tgatgatgat
     1021 ggccctggcg ttagccctga agatagagag catatcttcc gaccattcta tcgcactgac
     1081 gaagctagag atcgtgaatc cggtggtact ggcttaggat tagcgattgt tgaaactgcc
     1141 gtcagccaac acagaggcca tgttaaagcc gatgacagcc cattaggtgg gttgagggtt
     1201 gagatttggt tgccgagagc gggtgcaagt aacaaatcgc caacggctta atagagtaat
     1261 taaactgttg tatcaaaata tcctgccatc tattgaagaa taggtggcat attctttttg
     1321 ttcgcattta ttgatgcaat tcagaatgat ctggctgaat cgatcgctat tccgatattt
     1381 atatatctac aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt
     1441 ctactctcag tgcggttttt cgcatattgt taaagtatgc ttatataaaa aaacccttat
     1501 attacatata gataaggggt tttcgagatg aaatttggcg ctctgggggc cgtaaaccca
     1561 gtgcggtagc tataaaaaat taaattaatt attggataag gtatcaggat ttcatgaaat
     1621 tactaaaggg agctagtata tacctgattt ctaatatact aaactcagca attccctttc
     1681 ttctattacc aatactaact agatatttat caactgaaga atatggtaaa atagccatgt
     1741 tccaaatttt tttggccggc atcggaactc taattggttg taatacaata agtgcaatta
     1801 gtagaaaata ttttgatgat agaaagaatg ataatgagtt aaaagaatat aataactcat
     1861 gcctattcat cctatttata agttcagttt ttatttttat aattgtttct atctttataa
     1921 atcagttatc taacttctta tctatacctt cggattggat atttatagcg attataataa
     1981 gcactataaa ctatataaca tccttgagat taatacagtg gcaagttaga ggtgaagcta
     2041 aaaaatttgg tattcttcaa ttatcaaaca gtagcaccaa tataacttta tcattagttt
     2101 taattattat tttcaatcaa ggtgcacaag gaagaataga tgctcaattt attacatcta
     2161 ttttattttt tatattatct gcctttctcc tcataaaagg gaacttaata tctccaatac
     2221 caaaacccac gtattcatta gcaaaggatg ctttatctta tagcattcca ctattaccac
     2281 atacaatagg tgtattcttc attagtttta ttgataggtt cattattaat aaagaaatgg
     2341 gattaggtga agctggaata tatatggtag cagtacaact tagttcaaca atcgcaataa
     2401 ttttcaactc actctatcaa gcatatattc cgtggttatt tgatagattg aaaaaaaatg
     2461 atgaaaatga aaaaataaag atagttaaat atacatattt gttctttatt ttcctcattt
     2521 ttagtggttt aataatattt attatcagtc ctattatagt gaaatttcta gtaggtgagc
     2581 aatatcatcc aattactaat attattggtt ggttatgtat tggtcaaata ttcggtggta
     2641 tgtatcttat aatgtctgga tatatatatt attcgaaaaa aacatctcgc ctagccttaa
     2701 tcaccatatc atctggcatt actaatgtag tattattagt tgtatttgtg cctatgtatg
     2761 gattaatagg tgctgctgtt tctttttctt ttgtaaaaat agtacagttt atagctgtat
     2821 atttatcctc aataaaatgt gtaaatatgc cttggtttaa ttttacagga aatacaaatg
     2881 aatttcataa aaaaaattag aatagtcttt ctaataaagt taatatcaat tttaaaatta
     2941 tttataaaaa atgaaaaatt tagcaactta ttaaatttct ataactacaa gttaattaaa
     3001 gaaagattaa aaaacaggaa tgttttcata ggtaataata caattatata taatactgaa
     3061 ttctcatata gtcagaaagg agatcatttt tacataggta ataattgtac aattactggt
     3121 gcatatttac tgggacatga tgcttcaccg actttattta ttgatgattt aattgtacat
     3181 aaagatgtat ggaaaaatgg gtcaagaaga tcatatagag cacctataag aataggtgac
     3241 aatgtattta ttggtgtgaa tgctattatt ttacctggta tccatattgg agataatatt
     3301 gtcattgccg caggatctat cgtatcttgt gatttagaga gtaattttgt atatgctgga
     3361 aatcctgcaa aaaaaataaa gcctatagaa gagtatattg aaaaatataa aaatattttt
     3421 tattcaaacc cggaaagttt ttaatacata tgaatttata cagttataaa aataatttca
     3481 atcttgcaga ttttattgta ttattatttt ttttcttagc tccattatca attacttttt
     3541 ttgaaaagaa aaatttagct atccttattt ttttttctat ttcttttatt atcctaaata
     3601 taaaaaacat atataaaata aaaatagaaa aaatagcttt tacttttatt ctttcttatc
     3661 ttatctatat aactatttct agcataggta tgatttttaa ctttaataaa ttaaactact
     3721 cttatttagc taatttcttc tctcgactac tcacaataac aataggaata acattcttat
     3781 atataataac ttattggtca gaaagaaatt caaacaaatt aaaagtcata cctaaagtat
     3841 ctttttatat atgctttgtt tttttattat tagcatttta ccaaataata gcttttaaat
     3901 atcatctacc attctttgaa acaaggtcat ttatttatgg ttcaaatgct gcattaaatt
     3961 catcactagg ctttagaata acgtctattg ctagagaacc aaattattat tctcctttat
     4021 tattcgaatc attaataata gcttggattg tcttaaaaag aaaaagcttt cttatatttt
     4081 caatacttac aatcattata atgtataaga cttactctac aggtgtttat ttacatgctg
     4141 ctttattact agccatcctt tttctaacaa aaaaaactag ctctctattt aaattaacac
     4201 tgacaatagt atttttattc ttaatttcta taattatata tgttggatta gattcaacaa
     4261 gcttagagta cttttctgat aaattaagaa atgagttatc tggtgactcg acaagatcat
     4321 atgttattat aactataata actggatttc taggatgtca ctttataaat caactatttg
     4381 gcaatgggct aaatagcttg gatttttata atattttgtc aacaaacaat gttaaaaata
     4441 tagactttag tatttctaat aatttagtca tcgatttttt gtgggatgga ggaataatag
     4501 gtgttcttgt tttctctttt ggcttatttt atataggaaa actattattt aatagtttta
     4561 gaaaaaacaa atatttttat gcatcttttt tgttattttc tagtttttta attacatccc
     4621 tttaccgttc cgaatatgct tccttacatt ttttttggac tatagcaaat atagtcattt
     4681 tgtataaatt aggaaaggaa aataaaaatg aacattgata tgttatactc aatatatcga
     4741 gatggcattg tgaaattttt agaaaatatc ccttcaccta aagataatat aaagataatt
     4801 attgttcatc aagtgttacc tgaagataat aatgattatt tattagaacc attattatct
     4861 agtagaaacg atattcaata tattaaaagt tatactaaag gtgtaactaa aagccgaaat
     4921 atagccctct caaaagcaaa atcagacata gtactatttt gtgatgacga tgttaaatat
     4981 gtagataatt taaatgatat tattacctca gcttacgata aaaatccaaa gtatggcttt
     5041 ttaactttct cttatttaaa aaataatgaa tggcataaat ttaaaaaaca aaactttgaa
     5101 cataactata gaacaatcct taatattgga actatcgaag ttacttgtct aagaaaacat
     5161 atatttgata atcactttaa atttcctgaa gatttaggcg ctggtgcaaa atattttctt
     5221 tgtgatgagc ctgtatttat ttctcaattt ctaaagaaaa aattaaaagg gaaatatata
     5281 gctgaagtca taggagagca tcctgatgat tctagtggtc ttatcttcaa taatatttat
     5341 gcctttaaat caagaatact atgctttcaa cgaatatttg ggttgttgaa aggaactttt
     5401 ctatattact tatttttaat taaaaatttc aaaaaattta atggtttaaa atctgtaatt
     5461 aatgctttta tattggttca ctcaaaatga atattaaatt taaaaaattt ggtttaactt
     5521 ttaatgataa atggaatcat aaagatatta atttttttga tctattcaaa tttacttcat
     5581 ataaaggaca taataatttt agtacttcac tcccatttct aaaaaaacaa aaacggagta
     5641 tagttaatta cctttcattt gatgaagaca cactatggtc aaatgtaaga aaaaacacaa
     5701 gaaatgaaat taacaaaata aaaaaagaag atataaaata tagtattaat aatgtatccc
     5761 ttcatgaatt tataaattta tataatgaat ttatagattt taaaaattta tcactaacac
     5821 aactttcaga aaagaggtta gaaaaatata ataacaatat attattgacc tctacagaga
     5881 tatcgggtaa atggcaatca attcatgttt atttaatcat agataaatat tcagaactat
     5941 tatattcaat taccaatcac aatgctgatc acaaaaccac agggatggca aatagatttc
     6001 atcattggaa cgatcttatc cactttaaaa atcatgatta tgaagcatat gattggggag
     6061 gaattgcatt tggtgatatg gatggaattg caaaatttaa attatctttt ggtggtgaag
     6121 aaattatttc aaatatttat ttttccccat tatactatct aacattactt attagagaaa
     6181 aaccatggaa aaaataatta ttaatgccga tgattttgga atttctcaaa atgtaaataa
     6241 agctattgtc acttgttttg aacatgaaat aataaatagc acaacaatat tagctaatat
     6301 gtattctact aatgaagcta taaaactggc aaaagattta aatttatcag ttggtatcca
     6361 tttgaactta gtcgaaggtt gctctctaac atctaaaata aaaaacttct ctcaagttta
     6421 caataaagaa aatagtgcat ttgaatttaa tataagtaga tcatcattaa aattaaataa
     6481 cagtttatta gaagcaatca agatagaatt agatagccaa atatgctatt tattagataa
     6541 tggtatagat cctactcacc ttgattccca tcaacatact catacaatat ttccaatatt
     6601 taatattgtt aaatcattag ggaaaaaata taatttaaaa attagaattc caagaacaac
     6661 tggatcaaaa tcacagttaa agattattta taaaaaaatc atcatgttaa atttaatttc
     6721 taataagata aacctaacaa atgaatttat aaatatagat gaatttaaaa acggatatgc
     6781 tcataaaggt aacttagaag taatggttca cccagatata aatgaagcag gtgatatttt
     6841 ttgtaacaca accaatacta ttcttataaa aaaagaagac ttatgaaagc tctattgtta
     6901 aatacatcgt tttatcctaa tttcggtggt gtggaaaatt ctcttcgtag tatttgtaag
     6961 gttttatgtg aatcaggttg ggatgttgat attatcacga gtgatgatgg ctacctacca
     7021 agctctgaaa ttatatttgg tgccaatgtt tatagatata aacaacgaaa atttggttta
     7081 tttttatttt caataaaaaa tttattaaat aaaaataaag aaaaaaatta tgatttaata
     7141 atatccagac atacattaac atcccttgca ctattattaa acaacacaag aaattttcat
     7201 tttattgttc ctggggtaca ttactttcag aataataaaa aaaacagttc attgattgaa
     7261 aaagtaaagt ttatttccaa cattattatt gaaaaatatg tttttaataa agtatccaat
     7321 atttattgtt ttagtaaaac aatgaatgag caaatatcat tttatagaaa aaataaagag
     7381 attatgcaaa ctccacctgg agtagattta aataggttct ttcctgtaga taaagagatt
     7441 aaatcttcat taaggaagaa atataactta cctttaaata aaaaaataat attttgcttg
     7501 ggtcgatttg taaagttaaa aaactttgaa acaataatta agacaattta ttttttagat
     7561 gatgattatc acttattact agttggtaat ggcccattat taaaaaatta tttagaattg
     7621 attagcaaat taaatataga agataaagtt acaattataa aacccaccca aactccggaa
     7681 gaattttata gtactgcaga tattttttgc ttaccttcaa cctatgagcc ttttggacaa
     7741 gtattattag aagctactgc atgccattta ccaatagtag ctctcgatag tgattttttc
     7801 caaacagcaa gtaaagaaat atatcaagat tataataatc tgattacctt tactgatgga
     7861 aactcacctg aaaattttaa agaaaaaatc ttaaatttat caaaaaaaga ttttgataaa
     7921 actcaagcaa gttctttctt aaaaaaacac tcatggaata atctgataac taacattatc
     7981 aataatagta aaaatcatac ctctaagtat aagtaatata tattattaaa tatatttcat
     8041 gtaaaccata taaaacgcat actttaaaat caaggaatgt tatctaaaat gaaaatcacc
     8101 atctccggta ccggctatgt cggtctatct aacggaatac ttctttccca acacaacaaa
     8161 gttgttgcgt tagatatcaa taaagaaaaa gttgaattac ttaacaaaaa aatctcgcca
     8221 attacagaca aagaaattga agagttttta gcaaccaaag agctcgactt tacagcaaca
     8281 ttagataaag aatttgctta taaagatgct gaatttgtta tcattgccac accaaccgat
     8341 tatgatccta aaacgaacta tttcaacacc tcttctgtag agtctgtcat tcgtgatgtt
     8401 ttagaatata atccaaacac gacaatgatt ataaaatcaa ccattcctgt tggttttacc
     8461 aaagagatga gagagaaata taataccaat aatattattt tctcccctga gttcttacgt
     8521 gaaggtcgtg cgctttacga taacttatat ccatctcgta ttgtgattgg tgaacaatca
     8581 gaaagagcac aaaagtttgc tgacctttta caacaaggcg cactgaaaaa agatattgat
     8641 gttctgttca ctgactccac agaagccgaa gcgattaaat tatttgctaa tacttatcta
     8701 gcactccgtg ttgcttactt taatgagcta gatagctatg ccgaatctta tggcctaaat
     8761 gcaagacaaa tcattgaagg tgtctgctta gatccgcgta ttggcaacca ctataacaat
     8821 ccatcattcg gttacggcgg ttactgctta ccaaaagata ctaaacagct gttagccaac
     8881 tacgactctg tccctaataa tattattagt gcaattgtcg aagctaaccg cacacgtaaa
     8941 gattttattg cagactctat tattgcaaaa tcacccaaaa tcgtcggcgt atatcgttta
     9001 attatgaaat ctggctctga taatttccgt tcttcttcta tccaaggcat tatgaaacgc
     9061 attaaagcca aagggattga agtggtgatt tatgaaccag aaatgaaaga agagaccttc
     9121 tttaattcaa aattaattcg tgatttaaat gagtttaaag caatgtctga tgtcattatt
     9181 actaatagaa tgtcatcagc actaaacgat gttgaagaaa aagtttatac tcgtgacctt
     9241 tttggtaacg attaattata tatctacctt taagattagg ctgtaattct gatgttatcc
     9301 aaaaatacta attttttaat atctatcatt atgccatcct ataattcggg agaatatata
     9361 tgtaagtcta tagattccgt gctaagacaa acttataaaa attttgaatt gatcattatt
     9421 gatgattgct ctaatgataa tactattgaa atagtatctt cttacaaaga tgagaggatc
     9481 aaagtattaa aaaatgaagc taaccaaggt gctggaatta ctcgtaataa aggattatca
     9541 cttgctaatg ggcgttttat ttgcttttta gatagcgatg attattggcg agaggataag
     9601 ttagaaaaac agcttaattt tatgttatct aataagatag atttttgttt ttctgcttat
     9661 gatagagttt ctgaagataa cacttttatc tgtaaaattg gggttcctga aaaagtaagt
     9721 tattcagaca tgctaaaggt ctgttctatt ggttgtttaa cagtaatgtt tgataaagag
     9781 aaatttaaag atttttcaat gccatcaata agaaaaagac aagattatgc tctctggcta
     9841 aaactactga aacaaacacc ttatgcctat gggataaatg agtctcttgg ttattataat
     9901 ataagagaaa attcaatctc ttccaataaa cttaatgcag caaagtatca atggcgaatt
     9961 tatagagaag aagaaagatt aaatatcttt cattctattt attatttctc tcactatgct
    10021 ttcaatggtt tttttagatc taaatttcca tccttagcta aaaaaatagg tgttttaaaa
    10081 taactctcat ttttaatacc cagtattagg ctttatatgc tcaatatcgt cttatttgaa
    10141 cccgaaattc caccgaatac gggtaatatt atccgcctgt gtgctaacac aggctttcat
    10201 cttcatttga tccaaccatt gggttttact tgggatgata aacgtctacg tcgtgcaggg
    10261 cttgattatc gtgaatttgc tgatattaaa cagcaccacg attattatgc ttttttagaa
    10321 agtgaaggtt taaaccctga taatgcacaa tcaccatcag gtgcgcgtgt atttgcctta
    10381 accacaaaag gcacgccagc acatagcaat gtcagttatc aagatggaga ttatctcctg
    10441 tttggccctg aaacccgtgg tttaccacct tatgtattag ataatatgcc aacacagcag
    10501 aaaatcagag tcccaatgct tgctgatagt cgtagtatga atctgtcaaa tacggttgcg
    10561 gttgtggttt ttgaggcatg gcgccaactt ggttatcctg gtgcattact aagagactga
    10621 aatgcaaaaa gcgctgatca taaataatga ctagcgcttt ttaacttcaa ataactaaca
    10681 tgttaattta ttacattaag gattgtttta tttcagcaac aaccttcgtg atatatcgat
    10741 acccttcccc tacatccttc agtactaatg catgagcacc aatcaccgca ttatcaccaa
    10801 tctctatctc accgccgata atcgatgagt tacaaccaat ataaacgtta ttacccactc
    10861 gaatatccgc tttgccattc ttttctttta caccgattgt cacaccttgc attaacgtca
    10921 cattctcgcc tagctgacag taattagtta gtacgatccc tatgggatgt ggtaatgaaa
    10981 ggcctttacc tatgcgcgtt cttagtgaga tatcgttgca atattgacga ttgtttttag
    11041 caccaatacg aatgcctaat tttttgataa aacgattttt agaaaaataa aagaattggg
    11101 cgacacgcca ccaaaataga taacgtgaac gttggcagaa aaaaacacgg tgataaagtc
    11161 ttatccaact aaaaggtttg ttatcaccaa tatactcagc gtgtaaacaa gcctttagtt
    11221 cagaaagagt gacttcagtt aatgccatta aatgccatca ccacattcaa aaccgggtac
    11281 gacaccatta aagtgttgat ccatatcgag tgaaggcttg tcactggtcg gcttaccaac
    11341 aatgcgagca ggaacaccag caacggtagt gtgcgcagga acttcatgta aaacgactga
    11401 tcccgcacca attttagcac cgcgaccaat ctcgatattg ccgagtattt ttgcacccgc
    11461 accaatcatt acaccttcac gcactttagg atgacggtca ccgcaagttt tacctgttcc
    11521 acctaacgtc acagattgta aaatagaaac atcgttctct acgatagcgg tttcaccaat
    11581 cacaatgcct gttgcgtgat caagcataat accgtggcca atacgggcag ctggatgaat
    11641 atcaacacca aaggtcacag aaatctgatt ttgcagatac accgctaacg ctttgcgatc
    11701 ttgattccat aaccaatgcg cgatgcgata agcttgtaag gcatggaaac cttttaaata
    11761 aagcagtggt gtagagtatt tatcaacggc aggatcgcgt aaacgcacag ccgaaatatc
    11821 ataagctgcc gattcaatca tagaattatc actacgatat gcttcttcaa caatctcccg
    11881 aacagcaatc gcgggcattg tttgagttgc tagcttattt gccaacatat aacttaaggc
    11941 actacctaag ttttcatgtt tgagtaatgt cgcatgaaaa aaactcgcca aaattggctc
    12001 gcaatctgcc aagcatcttg cttcattttt aatatgatcc cagactcttt ttagctcttc
    12061 tagcgacata ctcactctca ttctttttac attgaagcct aagtaaatac ttaggcttct
    12121 tatgtttaaa acagaaataa ataactatct aaaatagact tagaacagat tgctgtctat
    12181 ctcatcttta gtagctcggc ctaataaaga taatgccgct tctttcgcgt ctttatgctg
    12241 atataaaatt tggtagatct gctcagtaat aggcatttca acaccaactt gttcggctaa
    12301 tgcgcgaact tctttagtat tacgatagcc ttcaactact tgtccaattt tttcttgggc
    12361 agtctccaca tcaaggcctt gacctaacat cataccaaat cgacgattac gagattggtt
    12421 atcggtacag gttaaaacta aatcacccaa ccccgccatg cccataaagg ttgcagcatc
    12481 tgcgcctaac gctttaccta aacggctcat ttcagcaagg ccacgagtaa ttaatgctgt
    12541 acgcgcattt gcaccaaacc ccataccatc agacatacct gcaccaatag caattacgtt
    12601 ttttaccgca ccacctaatt ggacaccgat aaagtcagga tttttatata ctcggaaact
    12661 tttgccacaa tggaacaact gttgtaactc ttcagagaag gtattgtccg ttgaagccac
    12721 agaaatcgca gtaggcagac ctgctgcaag ctctttggca aaagtagggc cagataaaac
    12781 tgcaagcggg atttcattac ctaatacttc acgcgctaca tcttgcaata agcgaccagt
    12841 atgagcctca agtccttttg tcgcccaaac aacacgtgca tcctgacgta aaaagggttt
    12901 gatctgctgt aatacatcac caaaaacatg gcttgggatc acaactagaa tattacggct
    12961 agcttcaatg gctttttgca aagaagcttc catgtataaa ctatcaggaa aggaaacatc
    13021 gggcagaaag gcctgattac agcgagcttg ctctaacgca gcaacatgtt caggatcatg
    13081 accccaaagt acaacatcat ggccattgcg cgctaaggta atcgctaaag cggtgccgta
    13141 tgaaccggca ccgataactg tcatagaagc gttcattaag cttcttcagc cccttcatta
    13201 gcggcatcag tttgttgctg ttgtaaatag ttcataaaca acgcatcaaa gttaactggt
    13261 gctaagttca gttgtgggaa agtaccacgg ctgactaagt tagtgataca ttcacgagca
    13321 taagggaaca gaacgttagg gcaatatgca cctaagcaat gtgctaattg agtgccttca
    13381 ataccttcaa cggtgaagat acctgcttgt tgcacttcac acaggaatgc agtttcgtta
    13441 tccatagttg ccgttacagt aacgcgcaga ataacttcat agacgttttc agctaaagtg
    13501 ttagaagaag tatctaaatc taatttaact tctggttgcc actctttttg gaaaacttgt
    13561 ggggcattag gcgcttcaaa agagatatct tttgtataga tacgttgaat ttgaaaagtc
    13621 atctcttggt tttgctgttc tgacat
//
