LOCUS       KY710733               14541 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2669 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710733
VERSION     KY710733.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2669"
                     /serotype="O71"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..1227
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..14541
                     /note="O antigen gene cluster"
     CDS             1293..2186
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00043.1"
                     /translation="MNKYLKILRNKLRYKYCDTFPELVTKSIYKERMRKTLNLDFPQT
                     FNEKLQHLKLNLYKNNPLVTKCADKYAAREYVKDCGCEEILNEIHQVWDQPDDINFSE
                     LPNKFVLKCNHGAGYNIICRDKDKISPDNIRQTLTTWLNEDYWRLAVEFVYKDISKKI
                     ICEKFIETKNNELPYDYKLFCFHGEPLFVMICTERESQHPKFYFVNKEWELLPYGIDY
                     KNSEVSTVLDKPQNYQKLFEYAEKLSKPFPFVRVDLYLNSGVIHFGELTFIPSAGMDK
                     ELNNEENKNVDLIFGNYLKLN"
     CDS             complement(2197..3186)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00044.1"
                     /translation="MKNTYKISVLMSVYNDSIHLNEAIDSILNQTFKDFEFIIINDGS
                     TDLSIDILKEYEEKDSRVKVYTNASNIGLAKSLNKGFLLASGDYIARMDADDYSYPTR
                     LQKQFEFMEDHKNIMVCGTSMEIYEQDDKFMILPTSHNAIAAGLIFGTNFYHPTVIFR
                     SDFIKQNKIVYPEDYQYAQDYGLWVNLLKLNQENIFSNLEEALLKYRIHPERNRKNYF
                     STQTSFAIKAQINALSMFNIEASKNVLYNLNFSNEELSLCNIKECDVILDKIRTKNKE
                     LCPYFYKNLEVAIVRKKVSLFRRCEKKSLFYPYMKVKARLIYLMNRKMIKALN"
     CDS             3723..5048
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00045.1"
                     /translation="MFYLIKKIMKKNILLKQSFIYLSSNILNASIPFVLLPVFSYYLS
                     TEEYGKIAIFQILLSGLNAIIGLNTTGAASRKFFDTKDNKILIQYNNSSFIILFLSSI
                     VIFILFYLFSDSFISFFNIPKEWLVFAIFSSTLDYILRFKLTQLQIRNKAFQYGAIQV
                     SNSFLSFTISIFLIVFLNFGAEGRIYGILITSFVTAILCFISLYKNNLLSTKESSLFY
                     IKDNLYFGIPLAPHILGVFLLSSIDRFIINKEYGLSEAGIYMMAVQVSLAIILVFDAL
                     NKTFMPYLFQQLTNVNMNTEIKNKIIIYSYIFFIFCIISGLLASIISPYIISFIAGSK
                     YLKAQEIVFWLCIGQSFQGLYLVVTNYITYVKKTYLLSLITIISGCINIILIFILIRK
                     YELIGVSYAFCISMFIRFILTWAVSNWVYPMPWNILKLTYFNNKHKKRI"
     CDS             5048..6136
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00046.1"
                     /translation="MKKILFIIPSLTGAGAERIITHLINNINKEKFNIELLLVFDVKH
                     TFLEKITSNIKIYELKKNKSLKFCLFDILKSVKKIKPDTVFIGSGHLGLLLSPLIIFN
                     KKVKWIVRETNFASLNTTKYLAKVFYNLFYKNYDLIIAQCDDMRKDLINIFNIPSHKI
                     HTINNPIDTDYIDSQLSCNINEKIELSENKINIIACGRLTYQKGFDLLLKNISKKKLS
                     SNKYHLTIIGVGENDKRDISIKLHKIVVKYNLESIVSFVGFKDNIYNWLEKADVFILS
                     SRFEGFPNILLEAIYCGTPAISNICPGGINEIINESNGIVFDIEKELVADKIEELLNI
                     NFSRETLKKSIKNKYGIKEIIYLYEKIL"
     CDS             6133..7218
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00047.1"
                     /translation="MIFHFIIYNFILLFSTFFAYLYSKENNKALSYFFILTSLLIIII
                     PTILRYDVGPDYKGYIGIFTEIQKGIDKPWYVEYLFYYMSNLFSFSKHGYVYVLGGYF
                     ICSIILILNLSSKNNIHYVLLFFITLNVGYFTFDDQVRQALAMSIFAYSMKYIKSNEF
                     IKYTIICLIASICHTSALLMIPFFYIVKIKWPKHIMLIIILVSIISFYLGWINIVIKQ
                     LFSILPIYKNYAYNNNYTNVGEAVGSGLGVLLNVFIFSICILSSNKKINYKLVNFIFF
                     GIILLLISSGNLNISRMSKYFLIFSIFVLADILSTSKDILFKISITIFLILFFQISIY
                     KRHAVNLEYQSIFNVNYEHNITRDRDY"
     CDS             7221..8030
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00048.1"
                     /translation="MKVEFLVATTNRTDLKFIDRIFQNINIDDIYILVINQCIDIPLP
                     SKIDIPHDNIRVLSVTEKGTSVSRNLAIKNMIGDICTFTDDDLIYEKGIIEKIINNFQ
                     QKNTDIITFKTGIINSNKDIKYYKNNHFKHTFRSLLSIGDWEIFFKSNIIKAKNILLD
                     ERFGLGCKYPACEYHIFITDCYKKGIDISYIPIVVVRHPDIISTGLKFTPILEISRGA
                     GFARILGYKSLIASIFFAIKKHPLYKEKHSFFKECYFLIKGNIKILLSKKK"
     CDS             8035..8772
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /gene="gt5"
                     /protein_id="AXZ00049.1"
                     /translation="MKNETFSIIMPAYNAEKTIESSIQSVFSQTYSDYILYIVNDCST
                     DNTKEILSKYNKNNKIVIINNNVNMGVAESRNLAINLCTGHYIAFLDSDDLWEKEKLA
                     QHLKYFNMGWNVVCSNYGTFNENNITNYRKSPEIFSFNDLLKTCHIGNLTGTYNAKKL
                     GKFYQKKIGHEDYLMWLTILKKCDKAYCIQQFLAKYRISNQSLSGNKYHAIKWQWNIY
                     RNELKLPLIKSVYYFFHYIFNAIKKRL"
     CDS             8804..9970
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00050.1"
                     /translation="MKITISGTGYVGLSNGVLLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSRLVRDLNEFKAMSDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             9996..11006
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXZ00051.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGEIVVGIDNLNDYYDVSLKES
                     RLNILNKLHNFSFSLIDLADREKIASLFETEKFDKVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNIKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINDEPIDIYNNGEMKRDF
                     TYVEDIVEGVARIADVIPVPQQDWKVSTGTPANSSAPYKVYNIGNGSPVNLMDYISAL
                     EAHLGKTADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             11010..11513
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00052.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYTFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11586..12143)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXZ00053.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIRQSSE"
     CDS             complement(12143..12976)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00054.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13055..14071)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00055.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGNVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(14071..14541)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5045 a   2353 c   2280 g   4863 t
ORIGIN
        1 atgctagaac aacatatcga agcggaatta gctcaagatc ccgctaacga tctcctttgg
       61 tggcgtcgat taattcgcgc tattgataaa tgggctccac ctggtcagcg tttaattatc
      121 gtgaccagtg aaggacgcat tatcggtgct caacgtaatg agatgcaggt tgtcagaaac
      181 tttattggtc aatctgataa cgccgatcac cctaaaaaga aaaaatatgg ccgttctgag
      241 atgttagggc cattttcagt aagagatggt gaagatcatt accagcttta tcttgttcgt
      301 ccatcaagta gccctcaatc tgactttatc aacttattat ttgataaacc gttattacta
      361 ctcatattta ccatgcttat cagcacaccg ttattagtgt ggctttcttg gagcctagca
      421 aaaccggcaa gacaacttaa aaatgcggct gatgatgttg cgaaaggtaa tttacgccct
      481 caccctgaac tagaaacagg ccctcaagaa tttttggcag caggtaccag ctttaatcag
      541 atgataagtg ctcttgaacg catggttgag gcacaacaac ggttaatttc tgatatttct
      601 cacgaactac gaacaccatt aacacgctta caattagcga gtgcattatt acgtcgtcgt
      661 agtggagaaa gtaaagagct agagcgtatt gaaactgaaa ctcagcgtct tgatggcatg
      721 attaatgatt tactcgttct ttcacgcaac cagcataaaa acgagttatt acgcgaaaca
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataatg ccaaatttga ggctgaacaa
      841 agtaataaga ccctacaagt cacaactcct ccgggagcat ggcctattta ctgcaatcca
      901 tattcacttg ctagtgcatt tgaaaatatt gttcgtaatg ctctacgtta ttcaaactct
      961 cgcattgagg ttgcatttac tgaacaaaac caaggtatca ctattattgt tgatgatgat
     1021 ggtccaggtg ttagtcctga agatagagag catatcttcc gaccgttcta ccgaacagat
     1081 gaagcaagag atcgtgaatc agggggtact ggcttggggt tagctattgt agaaacagcc
     1141 gtaagccaac atcgaggtca tgttaaagct gatgatagcc cattaggtgg gttaagggtt
     1201 gagatttggt tgccaaaaac aaagtaaaat ctatctaaat gtgattttta gccttaaaat
     1261 acgattttgc taaattatat tttattacct ttatgaataa atacctgaaa attttaagaa
     1321 ataaattacg ttataaatat tgtgatactt ttccagaatt agtaactaaa tcaatatata
     1381 aagaacgaat gaggaaaact ctaaatttag attttccaca aacatttaat gagaaattac
     1441 agcacctaaa gttaaattta tataaaaata acccattagt cacaaaatgt gcagataaat
     1501 acgctgctcg agaatatgtt aaagattgtg gttgcgaaga aatacttaat gaaatccatc
     1561 aagtatggga tcaacctgac gatattaact tctctgagtt gcctaataaa tttgtactaa
     1621 aatgcaatca tggtgcaggt tataatatca tttgcagaga taaagataag atatccccag
     1681 ataacattag acaaacatta acaacttggc taaatgaaga ctattggcga ttagctgttg
     1741 aatttgttta taaggatatc tctaaaaaaa taatttgtga aaaattcata gaaacaaaaa
     1801 ataatgaact tccatatgat tacaaattat tctgttttca tggtgaacct ttgtttgtca
     1861 tgatctgcac tgaaagagaa tcacaacacc ctaaattcta ttttgttaac aaagaatggg
     1921 aattattacc ttatggtatt gattataaaa atagtgaagt atctactgtt ttagataaac
     1981 ctcaaaacta tcaaaaactc tttgaatatg ctgaaaaact atccaaacca tttccttttg
     2041 ttagagtcga tttataccta aatagtggtg taattcattt tggagaatta acctttattc
     2101 cgtcagcagg tatggataaa gaattaaata atgaagaaaa caaaaatgtt gatctaatat
     2161 ttggaaatta cctaaaacta aactaagggc ggacccttag tttagagcct taatcatttt
     2221 cctattcatt aagtaaataa gccttgcttt gactttcata taaggataaa aaaggctttt
     2281 tttctcacaa cgccgaaata atgatacttt ttttcgcaca atagcaactt caagattttt
     2341 gtaaaaataa gggcaaagtt ctttattttt agttcttatt ttatccaaaa ttacatcaca
     2401 ttcttttata ttacataaac ttaattcttc atttgaaaaa tttaagttgt ataatacatt
     2461 tttgctggct tctatattaa acatacttaa cgcattaatt tgtgctttta tagcaaatga
     2521 ggtttgtgta ctaaagtagt tctttctatt tctttctgga tgaattctgt attttaataa
     2581 tgcctcttct agattagaaa aaatattttc ctgatttagc tttaataaat tgacccagag
     2641 cccataatct tgagcatatt gataatcttc tgggtataca attttatttt gtttaatgaa
     2701 atcagatcta aatatgactg taggatgata aaaattagtt ccaaaaatca atccagcagc
     2761 tattgcatta tggcttgttg gaagaatcat gaatttgtca tcttgttcat aaatttccat
     2821 cgatgtacca cagaccataa tgtttttatg gtcttccata aactcaaact gcttttgaag
     2881 gcgagttgga tatgagtaat catcagcatc cattcgagca atataatcac cagacgcaag
     2941 taaaaaacct ttgttcaaag atttagctaa cccgatatta gatgcattag tatacacttt
     3001 tactctgcta tctttctctt catattcttt taaaatatca atagacaaat cggtagaacc
     3061 atcattaatg atgataaatt caaaatcttt aaaagtctga tttaaaatag aatctatagc
     3121 ttcattcaaa tgtattgaat cattatatac tgacatcaac acgctgattt tataggtatt
     3181 tttcatttta cacttaaagt ttcttactct tataaacgat tactataaag gaaggaatga
     3241 gattagtgaa taccgccgtg attagaattc tgaagaagtt cttgcagtag catgagaata
     3301 acacactaaa gtaaataata accgataaca tggatcacga acagaaactt aaccattgga
     3361 aacaaacaaa tagagtaatt aaattgttgt atcaaaacat cctaccacct attgatgaat
     3421 aggtggcata ttcttttcat taacgtttgt tgatgtaatt cagaatgatc tggctgaatc
     3481 gacagctatt ccagtattta tatatcctca atcaatgcta aaatacccag ctattacttg
     3541 accgtctggt ttgaagagtc tactctcagt gcggttttta gcatattgat aaagtatgct
     3601 tatataataa agtctttata ttacaatgat ataaggattt ttcgagatga atcttggcgc
     3661 tctgggagcc gtaaacccag tgcggtagct tttctttgtt tatatagttt tttagctatt
     3721 aaatgttcta tttaatcaaa aagataatga aaaaaaatat tctattaaaa caatcgttta
     3781 tatatttatc ttctaatata ttaaatgcat ctattccttt tgtgttatta cccgtattta
     3841 gttattattt atctactgaa gaatatggca aaatagcaat ttttcagatt cttttatctg
     3901 gattaaatgc tattatagga ttaaatacca ccggagctgc tagtagaaag ttctttgata
     3961 caaaagataa taaaatacta atacaatata ataattcaag ttttattatt ctatttctaa
     4021 gttcaattgt tatatttatc ttattttatt tattttctga cagttttatt tcatttttta
     4081 acattccaaa agaatggctt gtatttgcta ttttttcatc cactctagac tatatattaa
     4141 gattcaaatt aactcagttg caaataagaa ataaagcatt tcaatatgga gcaattcaag
     4201 ttagtaatag ctttttaagt tttaccatat caattttttt aattgttttt ttaaattttg
     4261 gtgcagaagg tagaatttat gggattttaa tcacatcctt tgttacagct atcttatgtt
     4321 ttatttcact gtataaaaat aatttattgt caacaaaaga aagctctcta ttttatatta
     4381 aagataattt atattttgga attccactag ctcctcacat acttggggtt tttctattaa
     4441 gttctattga tagattcata ataaataaag aatatggttt atcagaggca ggaatatata
     4501 tgatggctgt tcaagttagc ttagctatta ttttagtctt cgatgcttta aataaaacct
     4561 tcatgccata tttattccaa caattaacaa atgtaaatat gaatacagaa ataaaaaata
     4621 aaatcattat ttattcatat attttcttta tattttgtat aatatcagga ttattagcat
     4681 ccataatttc tccatatata attagtttta tagctggttc taaatactta aaagctcaag
     4741 aaattgtctt ttggttatgt ataggacaaa gttttcaagg gttatattta gtagtaacca
     4801 actatatcac ttatgtcaaa aaaacatact tgctctctct aattactatt atatcaggct
     4861 gtataaatat aatattaatt tttatattaa tcagaaaata tgaactaatt ggagttagtt
     4921 atgcattctg catatctatg tttataaggt ttattctcac ttgggctgta tctaactggg
     4981 tatatcctat gccatggaac atattgaaac ttacttattt taataacaaa cataaaaagc
     5041 gtatctaatg aaaaaaatac tttttattat tccatcatta actggtgctg gagcagaaag
     5101 aattattact cacttaataa ataatattaa taaagaaaaa tttaatatag agttgttact
     5161 tgtttttgat gtaaagcata catttttaga gaaaataacc tcaaatatca aaatatatga
     5221 attaaagaaa aataagagtt taaagttttg cttatttgat atactgaagt cagtaaaaaa
     5281 aataaaacct gatactgttt ttattggttc aggtcattta ggcctacttc taagcccttt
     5341 aattatattt aataagaaag taaaatggat agtcagagaa actaattttg caagtttaaa
     5401 tacaactaaa tatttagcta aagtatttta taatcttttt tataaaaact acgacctaat
     5461 aattgcccag tgtgatgaca tgagaaaaga tctaattaat atatttaata taccatctca
     5521 taaaatccac acaataaaca atccaattga cacggattat attgactctc aattatcatg
     5581 taatataaat gaaaaaatag aactttctga aaataaaatt aatattattg catgtggtcg
     5641 attaacttat caaaaaggct ttgatttact attaaaaaac atatccaaaa aaaaattaag
     5701 ttctaataag taccatttga caattatagg agttggagaa aatgataaaa gagatattag
     5761 tattaagtta cataaaatag ttgttaaata taatttagaa agtattgtct cgtttgttgg
     5821 ttttaaagat aatatatata actggcttga aaaggcagat gtatttattt taagtagtag
     5881 atttgaaggt tttcccaata ttttattaga agcaatatac tgtggaacac cagctatatc
     5941 caatatatgc ccaggaggaa taaatgaaat tataaatgag agtaatggta ttgtttttga
     6001 tattgaaaaa gaattagttg cagataaaat agaagagcta ttaaatatta atttttcaag
     6061 agaaacacta aaaaaatcca tcaaaaataa atatggcatt aaagaaatca tataccttta
     6121 tgagaaaata ttatgatttt ccattttatc atatataatt ttatattact attttccact
     6181 ttttttgctt acctttatag taaggaaaac aataaagctt tatcttactt ttttatatta
     6241 acatcattat taattattat tattccaact atacttagat atgatgtggg gcctgactat
     6301 aaaggatata taggtatatt tactgagata caaaaaggaa ttgataaacc ttggtatgtt
     6361 gaatatttat tttattacat gagtaattta ttttctttta gtaaacatgg ttatgtgtat
     6421 gtgttaggtg gctatttcat ttgtagtatt attttaatac tcaatttatc tagtaaaaat
     6481 aatatccatt atgtattatt atttttcatt acattaaatg ttggatattt tacttttgat
     6541 gatcaagtaa ggcaggcatt agcaatgtca atttttgcct actcaatgaa atatataaag
     6601 tcaaatgaat ttattaaata tactataatt tgtttaattg ctagtatttg tcatacttca
     6661 gctcttttga tgataccatt tttttatata gtgaaaataa aatggcccaa acatataatg
     6721 cttattatta ttttagtttc aattatatca ttttatcttg gttggattaa tatagtaata
     6781 aaacagttat tttcaattct tcctatatat aaaaactacg cttataataa caattatact
     6841 aatgtaggag aagccgttgg tagtgggcta ggtgtattat tgaatgtttt cattttttct
     6901 atttgcattc tttcatcaaa taaaaaaatt aattacaaac tagtaaactt catttttttt
     6961 ggtataatac tacttttaat ttcatcagga aaccttaaca tatctagaat gtcaaaatat
     7021 tttctaatat ttagtatatt tgttcttgct gatatattat caacatctaa agatatttta
     7081 tttaaaataa gtataactat atttcttatt ctgttttttc aaatatccat atataaaaga
     7141 catgccgtta atctagaata tcaatctata ttcaatgtta attatgaaca taatataact
     7201 agagatcgag attattaaaa atgaaagtag aatttttagt agcaacaaca aacagaacag
     7261 atttaaagtt tatagataga attttccaga acattaatat tgatgatata tatatattgg
     7321 taataaatca atgtatagat atccctttac catcaaaaat agatatcccc catgataata
     7381 ttagagttct atctgtaact gaaaaaggaa catctgttag tcgaaacctt gctattaaaa
     7441 atatgatagg agatatatgt acatttacag atgatgattt aatttatgaa aaaggtataa
     7501 tagaaaaaat aattaataat tttcaacaaa aaaatactga tattattacc ttcaaaacgg
     7561 gtattattaa ctctaataaa gatataaaat attataaaaa taatcatttt aaacatactt
     7621 ttcgttcatt attatctatt ggtgattggg aaatattttt caaaagtaat attattaaag
     7681 ctaaaaatat cttattagat gaaaggtttg gattaggttg taaatatcca gcttgtgaat
     7741 atcatatatt tataactgat tgttataaaa agggaattga tatatcttat atacctatag
     7801 ttgttgtacg gcatcctgat attattagca ctggattaaa attcacccct attttagaaa
     7861 tttctagagg agctggtttt gccagaatac taggatataa atcattaata gccagtatat
     7921 tctttgcaat taaaaaacat cctttatata aagaaaaaca ttcttttttt aaagaatgtt
     7981 acttcttaat aaaaggcaat ataaagatat tactttctaa aaaaaaataa tattatgaaa
     8041 aatgaaacct tctcaattat catgccagct tataatgctg aaaaaactat cgaaagcagt
     8101 attcagtctg tattttcaca aacatattct gattatatat tgtatattgt aaatgactgt
     8161 tcaacagata atacaaaaga aatattatcc aagtacaata aaaataataa aatagttatt
     8221 attaataaca atgtaaatat gggtgttgct gaatcaagaa atttagcaat aaatttatgt
     8281 actggacatt atattgcatt tttagatagt gatgatttat gggaaaaaga gaaactagcc
     8341 caacacttaa aatactttaa tatgggttgg aatgtagtct gttctaacta tggaaccttt
     8401 aatgaaaata atattactaa ttacagaaaa tcacctgaaa tattttcatt taatgattta
     8461 ctgaaaactt gtcatatagg aaatttaact ggtacatata atgccaaaaa acttggtaaa
     8521 ttttatcaaa aaaaaattgg acatgaagat tatttaatgt ggttaacgat tttaaaaaaa
     8581 tgtgataaag cttattgtat tcaacaattt ttagctaaat atagaataag taatcaatct
     8641 ctttctggga ataaatatca tgccataaaa tggcaatgga atatttacag aaatgagttg
     8701 aaattaccat taatcaagag tgtgtactat ttttttcatt atatttttaa tgcaataaaa
     8761 aaaagattat aaataactct taacatttga aagtgatatt aatatgaaaa taaccatctc
     8821 aggaaccggc tatgtcggtc tatctaacgg ggtacttctt tcccaaaaca acaaagttgt
     8881 tgcgttagat atcaataaag aaaaagttga attacttaac aaaaaaatct cgccaatttc
     8941 tgataaagaa atcgaagaat ttttagcaac caaagagctc gactttacgg ctacattaga
     9001 taaagaattc gcttataaag atgctgattt tgttatcatt gcaacaccta ctgattacga
     9061 tcctaaaaca aactatttta acacctcatc ggtagagtct gttattcgtg atgttttaga
     9121 atataatcca aatacgacaa tgattattaa atcaaccatt cctgttggtt ttacaaaaga
     9181 aatgagagag aaatataata cggataatat tattttctca ccagaattct tacgtgaagg
     9241 tcgtgcactt tacgataatt tatacccatc tcgtattgtt attggtgaac aatcagaaag
     9301 agcacaaaaa tttgcagacc ttttacaaca aggtgcactg aaaaaagata ttgatgttct
     9361 gttcactgac tctacagaag cagaagcgat taaattattt gctaatactt atttagcact
     9421 gcgtgttgct tactttaatg aattagatag ctatgctcaa gcttatggct taaatgcaaa
     9481 acaaatcatt gaaggtgttt gcttagatcc tcgtatcgga aatcattata ataacccatc
     9541 gttcggttat ggtggttact gtttaccaaa agataccaaa caactgttag caaactacga
     9601 ctctgttcct aataatatta ttagtgctat cgttgaagct aaccgtactc gtaaagattt
     9661 tattgccgac tcaattatcg ctaaatcacc aaaaatcgta ggtgtttatc gtttaattat
     9721 gaaatctggc tctgataatt tccgctcttc ttctatccaa ggcattatga aacgcattaa
     9781 agccaaagga attgaagtgg tgatttatga acctgaaatg aaagaagaaa ccttctttaa
     9841 ctcaagatta gttcgtgatt taaatgagtt taaagctatg tctgatgtca ttattaccaa
     9901 tagaatgtca tcagtgctaa acgacgttga agaaaaagtt tatactcgtg acctatttgg
     9961 taacgattaa ttaataaata tgggttaatt acgtaatgaa atatttagtc actggtgcag
    10021 caggattcat tggttttcat ctaataaaaa aactaatcca acaaggtgaa attgttgttg
    10081 gtatcgataa tctcaatgat tattatgatg tctccttaaa agaatctagg cttaacattc
    10141 tgaataaact acataatttt tcattttctc ttattgatct agctgataga gaaaaaatag
    10201 ctagcctatt tgaaactgag aagtttgata aagttattca tttagctgca caagctgggg
    10261 ttagatattc actaattaac ccattttctt acgctgatag caatttaact ggctttctga
    10321 caatcttaga aggttgtcgt cacaacaata ttaaacatct tgtttatgct tcttccagtt
    10381 ctgtttatgg cttaaatgat gaattaccat tttcacctca tgatcaagct aatcaccctg
    10441 tttctttata tgcggcaacg aaaaaagcta atgaattaat ggctcatagc tattctcatt
    10501 tatatggtat accaacaact ggattacgct ttttcaccgt ctatggtcct tggggtcgcc
    10561 ctgatatggc attatttaaa tttacaaaag ctattatcaa cgatgagcct attgatattt
    10621 ataataatgg tgaaatgaaa cgagacttta cttatgttga agatattgtg gaaggtgttg
    10681 ctcgcatcgc tgatgtaata ccagtaccac agcaagattg gaaagtcagt acaggcactc
    10741 ctgctaatag ttcagcacct tataaagtct ataatattgg taatggttct ccagttaatt
    10801 taatggatta tattagtgca ttagaagctc acttaggaaa aactgcagat aaaaatatgt
    10861 taccaatgca acctggtgat gtttacacca catgggcaga cactgaggat ttatttaaag
    10921 caactggcta taaacctcaa acaagtgtag atgaaggcgt aaaacagttc gttgattggt
    10981 ataaaaatta ttatcaagtt aaataaacca tgctcaatat cgtcttattt gaacccgaaa
    11041 ttccaccgaa tacgggtaat attatccgcc tgtgtgctaa cacaggcttt catcttcatt
    11101 tgatccaacc attgggcttt acttgggatg ataaacgtct tcgtcgtgca gggcttgatt
    11161 atcgtgaatt tgctgatatt aaacagcacc acgattacta tactttttta gaaagtgaag
    11221 gtttaaaccc tgataatgca caatcgccaa ctggcgcacg tgtatttgcg ttaacgacaa
    11281 aaggcacacc agcacatagt aatgtgagtt atcaagatgg agattatctc ctttttggac
    11341 ctgaaacccg tggtttaccg ccttatgtgt tagacaatat gccaacacag cagaaaatca
    11401 gagttccaat gcttgcagat agccgtagta tgaacctatc aaataccgtt gcggttgtgg
    11461 tttttgaagc atggcgtcaa cttggttatc ctggtgcatt actaagagac tgaaatgtaa
    11521 aaagcgctga tcataaataa tgactagcgc tttttttaat ttcaaacgac taaaaatcta
    11581 acttattact ctgatgattg tctgatttcg gcgacaactt tagtaatata ccgacaccct
    11641 tcccctacat cctttaatac caatgcgtga gcaccaatca ccgcattatc accaatctct
    11701 atctctccgc cgataatcga agaattacaa ccaatataaa catggttgcc aactcgaata
    11761 tctgctttgc catccttctc ttttactcca atggtgacac cttgcattaa cgtcacattc
    11821 tcacctaact gacagtaatt agtcagtaca atccctatgg gatgaggtaa tgaaaggcct
    11881 ttacctatgc gcgttcttaa tgagatatcg ttgcaatatt tacgattgtt tttagcacca
    11941 atacgaatgc ctaatttttt aagaaaacga tttttagaaa aataaaagaa ttgggcgaca
    12001 cgccaccaaa atagataacg tgaacgttgg cagaaaaaga cgcgatgaaa tagtcttatc
    12061 caactaaaag gcttgttatc accaatatac tcggcgtgta aacaagcctt aagttcagag
    12121 agcgtaactt cagttaatgc cattaaatgc catcaccaca ttcaaatccc ggtaccacac
    12181 cattaaagtg ttgatccata tcgagtgaag gcttgtcact cgttggctta ccaacaatac
    12241 gggcaggaac accagcaacg gtagtgtgtg caggaacttc atgtaaaacg actgatcctg
    12301 cgccaatttt ggcaccgcga ccaatctcaa tattaccgag tattttagca cctgcaccaa
    12361 tcatcacacc ttcacgtact ttaggatgac gatcaccaca agttttacct gttccaccta
    12421 aagtcacaga ttgtaaaata gaaacatcgt tctctacgat agccgtttca ccaatgacaa
    12481 tgcccgttgc gtgatcaagc ataataccgt gaccaatacg tgcagcaggg tgaatatcga
    12541 caccaaaagt cacagagatt tgattttgca gataaacggc aagcgcttta cgatcttggt
    12601 tccataacca atgggctata cgataagctt gtagcgcatg aaaccctttt aaataaagta
    12661 gtggcgtaga gtatttatca actgcagggt cgcgtaaacg aacggctgaa atatcataag
    12721 ccgctgattc aatcatagaa ttatcgctac gatatgcttc ttcaacaatc tcacgaacgg
    12781 cgatcgcagg catcgtctgg gttgccagct tattcgccaa catataactt aaggcgctac
    12841 ctaagttttc atgtttgagt aatgtcgcat ggaagaaact cgccaaaatt ggctcacaat
    12901 ctgccaaaca tcttgcttca tttttaatat gatcccagac tctttttagc tcttctagcg
    12961 acatactcac tctcattctt tttacattga agcctaagta aaaacttagg ctttttatgt
    13021 ttaaaacaga aataaataac tatctaaaat agacttagaa aagattgctg tctatctcat
    13081 ctttagtggc tcggcctaat aaagataatg cggcttcttt cgcgtcttta tgctgatata
    13141 aaatttggta gatctgctct gtaataggca tttcaacacc gacttgttct gctaatgcgc
    13201 gaacttcttt ggtattgcga taaccttcaa cgacttgccc gattttttct tgggcagtat
    13261 caacatcaaa accttgacct agcatcatac caaatcgacg attacgagat tggttatcgg
    13321 tacaggttaa aactaaatcg cccaatcctg ccattcccat aaacgttgca gcatctgcac
    13381 ctaatgcttt acctagacgg ctcatttcag caaggccacg agtgattagt gctgtacgtg
    13441 cattcgcacc aaaccccata ccatcagaca tacctgcgcc aatcgcaatc acgtttttta
    13501 ccgcgccacc taattgcaca ccgataaagt cagggttttt atacacacgg aaacttttgc
    13561 cacaatggaa caactgctgt aactcttcag agaaggtatt gtccgttgat gcaactgaaa
    13621 tcgcagtagg cagacctgct gcaagctctt tggcaaaagt agggcctgat aaaactgcta
    13681 gcgggatttc attacctaat acttcacgcg ctacatcttg caacaagcga ccagtatgag
    13741 cctcaagtcc ttttgtcgcc caaacaacac gtgcatcctg acgtaaaaag ggtttgatct
    13801 gttgtaatac attaccaaaa acatggcttg ggatcacaac aagaatatta cggctggctt
    13861 caatggcttt ttgcaaagaa gcttccatgt ataaactatc aggaaaggaa acatcgggca
    13921 gaaatgcctg attacagcgt gcttgctcta atgcagcaac atgctcagga tcatgacccc
    13981 aaagtacaac atcatggcca ttgcgcgcta aggtaatcgc taaagcggtg ccgtatgaac
    14041 cggcaccgat aactgtcata gaagcgttca ttaagcttct tcagcccctt cattagcggc
    14101 atcagtttgt tgctgttgta aatagttcat aaacaacgca tcaaagttaa ctggtgctaa
    14161 gttcagttgt gggaaagtac cacggctgac taagttagtg atacattcac gagcataagg
    14221 gaacagaacg ttagggcaat atgcacctaa gcaatgtgct aattgagtgc cttcaatacc
    14281 ttcaacagtg aagatacctg cttgttgcac ttcgcacagg aatgcagttt cgttatccat
    14341 agttgcagtt acagtgacac gcaaaataac ttcatacacg ttttcagcta aagtattaga
    14401 agatgtatct aaatctaatt taacttctgg ttgccactct ttttggaaaa cttgtggagc
    14461 attaggtgct tcaaaagaga tatcttttgt atagatacgc tgaatttgaa aagtcatctc
    14521 ttggttttgc tgttctgaca t
//
