LOCUS       KY710734               14876 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2671 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710734
VERSION     KY710734.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2671"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
                     /note="genotype: O72ab"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..14876
                     /note="O antigen gene cluster"
     CDS             1572..1991
                     /note="glycerol-3-phosphate cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wffW"
                     /product="wffW"
                     /protein_id="AXZ00056.1"
                     /translation="MTNKENLKMKRVITFGTFDVFHVGHVNILERAAALGDYLIVGIS
                     SDQLNFSKKQRYPIYSQHDRTKIISSLKYVNEVFLEESLELKLEYIKKYKADVLVMGD
                     DWEGRFDWVKTDCEVIYLPRTPSISTTEIIEVVRSIK"
     CDS             1996..3210
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00057.1"
                     /translation="MQNKNILIYFASDLLAKAIPFLLIPLLTRYLSPTEYGDISILNI
                     AIEIITILILLGANTYFRVEFFKTSDKNALLYNLFSNVFINFVILTLVLIIVSFYFKN
                     ITFNIQNLIFISIISFFQSIFYIITSYYQCSEKSIIVGISNISFSIISNILFITLIIY
                     GLREEARYYSYFIGISLISITLLFKLTIKNKHNIKKPFIDKSYYKFGFSILPHAISWW
                     ARSGMERIIISYFMSNFYVGIYSLSLQFVSLLVIISNAVNQAIAPSMMLALNNKRKKE
                     INKLINKSLIINTLCCLSLSVFSIFFISFFISKEYMEVRYVLALMSLGYIFQSIISTY
                     SNVIYFFNNNLFLSKVTFISSFINIALVYLSMNYISSLYSVVIISIITYLITSIIIII
                     KSKKLIIKGTEC"
     CDS             3204..4010
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00058.1"
                     /translation="MLIKKIKNISLINISKLIFTIRSIYFRIKNNKKKKIFVFTDSRG
                     YEVTNPWNKKNPFSSYIGNLIKNYNVEYHICEESSTTIIDFLYSYKTQIKKGKKYDIV
                     IAQVGLVDFSPRPKNMLKKIIDKKNNKIKNINLDINYFYNRLNDNIFDEIYYNEELSN
                     LYSINYFESNIIPLLLSINNFIYIGCNPILHNWKGNYWKNRPKDINIILDYSKLLENC
                     LPSQNVISLLNWNEKEIMKRTVDNIHLNKYGYIYLDDILKEKINEVSQCN"
     CDS             4130..5173
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXZ00059.1"
                     /translation="MLKNKNIKYEIYWYTKNKNLYNELKSKNIPVIYGFRYKAIIKHI
                     RAQAIFCNCSPLSDLLGFAINKKTTVFNLWHGTPMKKIGNDAIKDLPKSNNLGIDKNK
                     IELILRKITPDNLIKALKPNVYYLASSSEIGDILSSAFSIKKDKIIINGYPKLDVSSE
                     TTEVKRMILYAPTYRGEYNSENDILTKNNFIPDIINQKLEDFNLTLTIRLHPANDLPK
                     NIEEKLLNYSNIILDKSKKDIYLDLYKYELIITDFSSIYFDALAMHKKVVIAPFNIST
                     YLEEDRSLYFTPDELFPYDRPNDWIELANNIQYYLSINVKSFYNLRERFYPHDISCSS
                     SNELLKKIIAILR"
     CDS             5176..6288
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00060.1"
                     /translation="MEINLIKNERYILLLCCCTFFSINYLPINLIYILVFFLFILGFS
                     HFLLKMTIKKYSIYFFLFPVLFLLSQFLGLHFNKFYFNNNVNILSPLLFFISWFLSFF
                     LLQGLPYIPKERRIIIYNKLIKYSIIFFILEFITRLFLYQGNKEFIYAFKKSFFYFDS
                     NFTGLVILSILAFSLYIEYRLKIKNKTKYILLLLIILTLSRSAIFTSFILIIITNNYL
                     LNFKSKSYFLLSIILIVFSYMSIEYIFFSGNYQSIDGSFNSKFYIVKEAFHLYSTLPI
                     ENKLFGIGLGNTDLFLNIFAHNIFVTMFLEFGIIGSLLFIIFIIYSLFNSNHYCLFIW
                     FPILIAGISLFGAYSPYLFIINAIIYFEENYYKLQS"
     CDS             6285..7223
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00061.1"
                     /translation="MKKTSPLISVIMPAYNVEKWIDEAIYSVLNQTITDLELIIVDDC
                     STDNTYNLIKNIALNDNRIKLFKNEKNSKICKTLNFALKQAKGIYVARIDSDDIAVPD
                     RLEKQLAYLNKKELDLVGSQMLAIDEYNNKLSYSKLPMGHLINKTKYLASPITHIWLC
                     KKEIYHTLNGYRNIPYAEDYDFILRALDFGYKCDNHPDALMCIRHRDGNTATTASLAQ
                     RKTHNYVLKLAQERAKNKTLTDSFTEENLQSYIKSSAIFTKLHNYSSLKLNKAIKEKN
                     KLKKLVYIIESSLISYYNFQYLFRRAIFYIIRKRIK"
     CDS             7220..8251
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00062.1"
                     /translation="MKNIYLLIDDLSNTGGTERVATIIANQLNKYDYNITIFSLSLIK
                     KEIFFPLDKNISIRNSNDKTLKFLEIIKILRLSSKNKATVIIISMGKLSFISSILSIL
                     FKPYKLILSEHISFESYNLLKRFLKKQSYKIANKVILLTKNDKDIINIKNSAVIRNIN
                     PYYQHNIQKYNSRKNIAIAIGRFTYQKNFERLIKLWNEANITNWDLMIIGKGENEEKL
                     KELSKNNTNIHIMPPNNRLDKIYNNAKLMLMTSRYEGLPMVLIEAQGFGLPIISFDCK
                     TGPAEIIKNNESGYLIDYNNDKEFIKKLIYLCNNDNELSRMNINAVLNSYHFSPEKII
                     TDWIKIIEE"
     CDS             8253..8987
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00063.1"
                     /translation="MEHELISIIMPAYNAEKTIKESIESILNQTIIDFRIYIIDDNST
                     DTTSEIIHSFNDKRIIYIYNDSNQGVSKSRNIGIKLSKGKFIAFLDSDDLWAPNKLEE
                     QYKKLINGWDVVCSNYTTFDETNQIKHRVSPEIINYSDMLLSNFIGNLTGVYNKDNIG
                     KVYQKNKGHEDYIMWLDILKKSKKAYCIQKPLAKYRISEKSLSGNKLKAMQWQWSIYR
                     NELNLSFIKSSYYFINYIYNALKKRK"
     CDS             8990..9937
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXZ00064.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSEKYPEKWIFG
                     DVTKPETLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWYKKDNTNTLIVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATKIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHTHIKVPYAIGLLGGYCFDVLSKVTGKEFPVSSIR
                     IKKFCARTQFKSNSITNTDFKAPVTLQQGIANTVKNEFL"
     CDS             10169..11335
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00065.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             11347..11850
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00066.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11921..12478)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXZ00067.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRIYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIKQSSM"
     CDS             complement(12478..13311)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00068.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13390..14406)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00069.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(14406..14876)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5333 a   2433 c   2242 g   4868 t
ORIGIN
        1 atgctagaac aacatatcga agctgaatta gctcaagatc cggctaacga tctcctttgg
       61 tggcgtcgat taattcgagc cattgataaa tgggctcccc ctggccaacg tttaattatt
      121 gtcacgagtg aaggccggat tatcggtgct caacgtaatg agatgcaagt tgtcagaaac
      181 tttattgggc aatctgataa cgccgatcac cccaaaaaga aaaaatatgg tcgctctgag
      241 atgctagggc ctttttctgt aagggatggt gaagatcatt accaacttta tctcgtgcgc
      301 ccttcaagta gccctcaatc tgactttatc aacttattat ttgataaacc attattgtta
      361 ctcatattca ccatgcttat cagcacacca ttattagtat ggctttcttg gagcttagca
      421 aaacccgcaa gacagcttaa aaatgcggct gacgatgtgg ctaaaggtaa cctacgccct
      481 caccctgaac tagaaactgg ccctcaagag tttttagccg caggcacgag ttttaatcag
      541 atgataagtg ctcttgaacg tatggtagag gcgcaacaac gtctgatctc tgatatttct
      601 catgaactac gtaccccatt aacacgctta caattagcca gtgcattgtt acgtcgtcgt
      661 agtggagaga gcaaggagct agagcgtatt gaaactgaga ctcaacgcct tgatggtatg
      721 attaatgact tactagtact ttcccgtaat cagtataaaa acgagttatt acgcgaaaca
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataacg ctaaatttga ggccgaacaa
      841 agtaataaga cactgcaagt cacaacccct ccaggagcct ggcctattta ctgcaatcca
      901 tattctctgg cgagtgcatt tgagaatata gtccgtaatg ctctgcgtta ttcaaattct
      961 cgtattgaag tcgcgtttac tgaacagaac caaggtatca ccattatcgt tgatgatgat
     1021 ggccctggcg ttagccctga agatagagag catatcttcc gaccattcta tcgcactgac
     1081 gaagctagag atcgtgaatc cggtggtacg ggcttaggat tagcgattgt tgaaactgcc
     1141 gtcagccaac acagaggcca tgttaaagcc gatgacagcc cattaggtgg gttgagggtt
     1201 gagatttggt tgccgagagc gggtgcaagt aacaaatcgc caacggctta atagagtaat
     1261 taaactgttg tatcaaaata tcctgccatc tattaaagaa taggtggcat attctttttg
     1321 ttcgcattta ttgatgcaat tcagaatgat ctggctgaat cgatcgctat tccgatattt
     1381 atatatctac aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt
     1441 ctactctcag tgcggatttt cgcatattgt taaagtatgc ttatataata aaattcttat
     1501 attacatata gataagggtt tttcgagata aattttggcg ctctgggggc cgtaaaccca
     1561 gtgcggtagc tatgactaat aaagagaatt taaaaatgaa acgtgttatt acgtttggta
     1621 catttgatgt atttcatgta ggtcatgtaa atatcttgga aagagccgct gcattaggtg
     1681 actatttaat tgttggaatt tcctcagatc aattaaattt tagcaaaaaa caaagatatc
     1741 ctatttacag tcagcatgat agaactaaaa taatatcatc actaaagtat gtaaatgaag
     1801 tctttttaga agaatcatta gaactaaaac ttgaatacat taaaaaatat aaggctgatg
     1861 ttttggtcat gggagacgat tgggaaggaa gattcgactg ggtaaaaact gattgtgaag
     1921 ttatttattt accaagaaca ccctcaatat ctactactga aattattgaa gttgttagaa
     1981 gtataaaata ataacatgca gaataaaaat atactgatat acttcgcaag tgatttacta
     2041 gccaaggcga ttcccttttt attaattcca ttattaacta gatatctatc tccaacagaa
     2101 tatggggata tttctatatt aaacattgct attgaaataa tcacgatact cattcttttg
     2161 ggagctaata catacttcag agttgaattt tttaaaacat cagataagaa tgctctttta
     2221 tataatttat tttctaatgt ttttattaac tttgtaatat taacactagt tttaattatt
     2281 gtttcatttt attttaaaaa cataacattt aatatacaaa atcttatatt tatttctata
     2341 atctcttttt ttcaatctat attctatatc ataacttcat attatcaatg ttctgaaaaa
     2401 tctattatag ttggcatatc taatatttct ttttctataa tatcaaatat tctttttata
     2461 acactcatta tatatggctt aagagaagaa gccagatact attcatattt tattggaata
     2521 tctttaatat caatcacctt gttatttaag ttaacaatta aaaacaaaca taatataaaa
     2581 aaaccattta tagataaatc ctactataaa tttggattta gtattttacc acatgcaatt
     2641 agttggtggg caagaagtgg catggaaaga ataataattt cttattttat gagtaacttc
     2701 tatgttggga tatactcttt atcattacaa tttgtttcat tattagtaat aatttcaaat
     2761 gctgtcaatc aagctatagc cccatcaatg atgctagctt taaacaataa aagaaagaaa
     2821 gaaataaata aattaataaa taaaagttta atcataaata cattatgctg tttatcactc
     2881 tcagtttttt ctattttctt tataagtttt tttatctcaa aagagtacat ggaagttaga
     2941 tatgttttag ctttgatgtc gttaggatat atattccaat caattatttc cacatatagt
     3001 aatgttatat attttttcaa taacaattta tttttatcaa aggtcacctt tatttcctca
     3061 tttataaata tagctcttgt ttatcttagt atgaattata taagcagtct atatagtgtg
     3121 gtaattatct ctataataac gtatttaatt acatcaatta taattataat aaaatcaaaa
     3181 aaattaatta tcaagggtac tgaatgttaa taaaaaaaat aaaaaatata agcttaatta
     3241 atatttcaaa gcttatattt acaataagat ctatctattt tcgtataaaa aataacaaaa
     3301 aaaagaaaat ttttgttttc actgactcaa gaggatacga agtaactaat ccatggaata
     3361 aaaagaatcc cttttcatca tatattggta atttaataaa aaattataat gttgaatatc
     3421 atatatgtga agaaagtagc acaacaatta ttgacttcct atattcatat aaaactcaaa
     3481 taaaaaaagg gaaaaaatat gatatagtta tcgctcaagt gggtctagtt gatttttctc
     3541 ctagacctaa aaatatgcta aaaaaaataa ttgacaaaaa aaacaataaa ataaaaaata
     3601 taaatttgga tattaattat ttttataatc gtttaaacga caatattttt gatgaaattt
     3661 attataatga agaattaagc aatctctatt caataaatta ttttgagtca aatattatac
     3721 ctttgttatt atcaattaat aattttattt atattggttg caatccaata ctgcacaact
     3781 ggaaagggaa ttattggaaa aatagaccta aagacataaa tataatatta gattatagca
     3841 aattactaga aaactgccta ccttcccaga atgtgatatc attattgaac tggaatgaaa
     3901 aagaaataat gaaaagaact gttgataata ttcatctaaa taaatatggc tatatttatt
     3961 tagatgatat tttaaaagaa aaaattaatg aggtatcaca gtgcaattaa tactaagagt
     4021 tattttttca ttcatatctt actttattcc aataaagaaa aatcttttta tatttggaag
     4081 ttggcattct cttcgatatg gagataaccc taaagcttta ttcgaattta tgctaaaaaa
     4141 taaaaatata aagtatgaaa tatattggta tacgaaaaat aaaaatttat ataacgaatt
     4201 aaagagcaaa aatattcctg ttatttatgg ttttagatat aaagcaataa taaaacatat
     4261 aagagctcaa gctatttttt gcaattgctc accactatca gatttattag gttttgctat
     4321 taacaaaaag accacagtgt ttaatttatg gcatggcact ccgatgaaaa aaattgggaa
     4381 cgatgcaatc aaagatctcc ctaaaagcaa taatcttggt atagacaaaa ataaaattga
     4441 attaatctta agaaaaataa cacctgataa tttaattaaa gctttaaaac caaatgttta
     4501 ttatttagca agctcttcgg aaattggaga tattctttcc tctgctttta gtattaaaaa
     4561 agataaaata ataataaatg gttatcctaa attagatgtg tcttctgaaa caacagaagt
     4621 aaaaagaatg attttatatg caccaacata taggggtgaa tataatagtg aaaacgatat
     4681 tctaacaaaa aacaatttca tccctgatat aataaatcaa aaattagagg attttaactt
     4741 aactttaact atacgtctgc atcctgctaa tgatttacct aaaaatattg aagaaaaatt
     4801 attaaactat agtaatataa tcttagataa atcaaaaaaa gatatttatt tagacctata
     4861 taaatatgaa ttaattatta ctgatttctc tagcatatat tttgatgctt tagccatgca
     4921 taaaaaagta gttatagccc catttaacat atcaacttac ctagaagaag atagatcttt
     4981 atactttact cctgatgagt tatttcctta cgatagacca aatgattgga ttgagttagc
     5041 aaacaacatt caatattact tatcaattaa tgtaaaatct ttctataatc ttagagaaag
     5101 gttttatcct catgatattt catgttcatc atctaatgaa ttattaaaaa aaataatagc
     5161 tatattaaga taaaaatgga aattaattta attaaaaacg aaaggtatat attattatta
     5221 tgttgctgta catttttcag cattaactat ttacctataa atttaatata tatattagta
     5281 ttttttttgt ttattttagg attttctcac tttcttttaa agatgaccat aaaaaaatac
     5341 tctatatatt tctttttatt cccagtatta tttttacttt cccagttctt aggtcttcat
     5401 tttaacaagt tttattttaa caacaacgtt aatatccttt ctcctttgct tttttttatt
     5461 tcatggtttt tatctttttt tcttctacaa ggactaccct atatacctaa agaaagaaga
     5521 attattattt ataataaact catcaaatac tctattattt tttttatttt agaatttatt
     5581 actagactat ttttgtatca aggaaataaa gaattcatct atgcatttaa gaagagtttt
     5641 ttctattttg actctaactt taccggttta gttattcttt ccatattagc ttttagttta
     5701 tatattgaat atagattaaa aataaaaaat aagacaaaat acattctttt actactgatt
     5761 atattaactc tctctcgtag tgcaatattt acatccttta ttctaattat tattacaaac
     5821 aattatttat taaactttaa atctaaatct tattttctac ttagtataat tttaatagtc
     5881 ttttcttaca tgtctattga atatatattt tttagtggga attatcaatc tattgatggt
     5941 agtttcaact ctaaatttta tattgtaaaa gaagcatttc atttatatag tacgcttcct
     6001 atcgaaaata aactatttgg aattgggctt ggtaatactg atttatttct caacatcttc
     6061 gctcacaata tttttgttac aatgttttta gagtttggaa ttataggaag cctattattt
     6121 ataattttta ttatatactc attatttaac tcaaatcatt attgtttatt tatttggttc
     6181 cctatactaa ttgcaggtat atcacttttt ggtgcttatt ccccctattt atttatcata
     6241 aatgccatta tttattttga agaaaattac tataagttac aatcatgaaa aaaacatccc
     6301 ctcttatatc tgtcataatg cctgcatata atgtagaaaa atggatagat gaagctatat
     6361 atagtgttct taaccaaaca ataactgatc tagaattaat tattgtagat gattgttcta
     6421 ctgataacac ctataactta ataaaaaata ttgctcttaa cgataataga attaagttat
     6481 ttaaaaatga aaaaaattct aagatatgta aaacgcttaa ttttgcatta aaacaagcta
     6541 aagggatata tgtagcaaga atcgattcag atgatattgc agtacctgat agattagaaa
     6601 aacaactagc ttatttaaat aaaaaagaac ttgatctagt tggctctcaa atgttagcaa
     6661 ttgatgagta taataataaa ttatcatata gtaaattacc gatggggcat ctaatcaata
     6721 aaacaaaata tctagcatca ccaatcaccc atatatggtt atgtaagaaa gaaatttatc
     6781 atacacttaa tggatataga aatattcctt atgctgaaga ttatgatttt atattgagag
     6841 cattagactt tgggtataaa tgtgataatc atccagacgc tttaatgtgc atcagacata
     6901 gagatggcaa tactgcaact acagctagct tagcgcagag aaaaacacac aactatgttc
     6961 tgaagttagc tcaagagcga gcaaaaaata aaactttaac agatagtttt acagaagaaa
     7021 acttacaatc atatataaaa tcatcagcaa tatttaccaa acttcataat tattcatctc
     7081 ttaaattaaa taaagcaata aaagaaaaga ataaattaaa aaaactcgta tatataatag
     7141 aatcatcatt aatctcatat tataattttc aatatttgtt tagaagagct attttttaca
     7201 taataagaaa gagaataaaa tgaagaatat ttacttatta attgatgatt tatcaaatac
     7261 aggtggaact gaaagagtag caacaataat tgctaatcaa ctaaataaat acgattacaa
     7321 cataactata tttagtttat ctctaataaa aaaagaaata ttttttcctt tagataaaaa
     7381 tatttcaatt agaaattcaa atgacaaaac acttaaattt ttagaaatta taaaaatact
     7441 aagattatca tcaaaaaata aagctacagt tataataatt tcaatgggaa aattatcatt
     7501 tatatcatca attttatcca ttttatttaa accttacaaa cttattctat ctgaacatat
     7561 atcttttgaa tcttataatt tattaaaaag gtttttaaaa aaacaatcat ataaaattgc
     7621 taataaagta atattactta ctaaaaatga taaagatatt attaacataa aaaactcagc
     7681 tgtaataaga aatataaacc cttattatca acacaatatt caaaaatata attcaagaaa
     7741 aaatattgct atagcaattg gtcgatttac ttatcaaaag aattttgaac gtttgatcaa
     7801 attatggaat gaggcaaata ttactaattg ggatttaatg attataggaa aaggagaaaa
     7861 tgaagagaaa ttaaaagaac tctcaaaaaa taatactaac atccatataa tgccgccaaa
     7921 caatagatta gataaaattt ataataatgc aaaattgatg ttaatgactt ctcgttatga
     7981 aggtttacca atggttttaa tcgaagctca aggttttgga ttacctatta tttctttcga
     8041 ttgcaaaaca ggaccagctg aaatcatcaa aaacaatgag tctggctatt tgatagacta
     8101 caataatgat aaagaattta taaaaaaatt aatatacctt tgcaataatg ataatgaact
     8161 ctcaagaatg aatattaatg cagttctaaa ttcatatcat ttctcacctg aaaaaatcat
     8221 aaccgattgg ataaaaataa tagaagaata acatggaaca cgaattaatc tcaattataa
     8281 tgcctgcata taatgcagaa aagaccataa aagaatccat tgaatcaata ttaaaccaaa
     8341 caataataga ttttagaatt tatataattg atgataactc aactgataca acatcagaga
     8401 taattcattc attcaatgat aaaagaataa tatatattta taatgatagt aaccaaggtg
     8461 tttccaaatc tagaaatatt ggaataaaat tatctaaagg taagtttatt gcatttttag
     8521 atagtgatga tctctgggct cctaataagt tagaagagca atataagaaa ctaattaatg
     8581 gctgggatgt tgtttgttcc aattacacaa cctttgatga aacaaatcaa ataaagcata
     8641 gagtctctcc tgaaataatc aattactctg atatgctttt atctaacttt attggcaatc
     8701 ttacaggagt ttataataag gataatatag ggaaagttta tcaaaaaaat aaaggtcacg
     8761 aagattatat catgtggcta gatattttaa agaaaagtaa aaaagcatac tgtatccaaa
     8821 aaccactagc taaatataga atatctgaaa aatccttatc aggaaataaa ttaaaagcaa
     8881 tgcaatggca atggtctatc tatcgaaatg aattaaattt atcttttata aaatcatctt
     8941 attattttat taattacatt tataatgctt taaagaagag aaaatagcta tgctaacaat
     9001 catcggtggt tcaggtttta ttggcacacg cctttcagat caactaacta aagaaaatat
     9061 tgaatttaaa attgtagata ttgctaaaag tgaaaaatat cctgaaaaat ggatatttgg
     9121 tgatgtaaca aaaccagaaa cattacttga accattaaaa ggctctgatg ttattatcaa
     9181 cttagcggca caacataaag ataatgttca tcctattagt ttatattatg aagttaatgt
     9241 agatggagct aaaaatgttt gtgatgttgc tgaacaacta aatattaaac atatcgtatt
     9301 tacttcatct gttgctgtat atggatttgt tgaaaaagaa acgggtgaag atggtgagtt
     9361 tcatccattt aatgattatg gaaaatcaaa acttgaagct gaacataagt atgaagcttg
     9421 gtataaaaaa gataatacaa atacattaat cgttatcagg ccaaccgttg tatttggtga
     9481 aggcaataga gggaacgtat ataacctatt ccgccaaata gcatcgggta aatttttaat
     9541 ggtgggttca ggtaataata aaaaatctat ggcttatgta gaaaacatcg ctgctttctt
     9601 aaaatttgct actaaaatag aatctggtca tcatatattt aattatattg ataaacctga
     9661 ttttactatg aatgaattga cagatatcat ttatacagct ctagataaac gacataccca
     9721 cattaaagtt ccttatgcta ttggattact tggtggatat tgttttgatg ttctttctaa
     9781 agttacaggt aaagaattcc ctgtaagcag tattcgaatc aaaaaattct gtgcccgcac
     9841 tcaatttaaa tcaaatagta ttactaatac tgattttaaa gcgccagtta ctcttcaaca
     9901 aggtattgct aacacagtta aaaatgaatt tttataatag attaatttta aatagtcttt
     9961 gtattaacaa ataattaaaa tacaccctac acttctctac ttattttcta ataaaaaatt
    10021 aattttacat taatttaata agtatatgat ccttatgatt gctaaatata ctataccttt
    10081 actaaagagt gagaattaac actaaaatat ctatttttat tctagtatag tgaaattata
    10141 aatacatata ctttagataa gagaaaccat gaaaatcacc atctccggta ccggctatgt
    10201 cggtctatct aacgggatac ttctttccca acacaacaaa gttgttgcgt tagatatcaa
    10261 taaagaaaaa gttgaattac ttaacaaaaa aatctcgcca attacagaca aagaaattga
    10321 agagttttta gcaaccaaag agctcgactt tacagcaaca ttagataaag aattcgctta
    10381 taaagatgct gaattcgtta tcattgctac accaaccgat tatgatccta aaacgaacta
    10441 tttcaacacc tcttctgtag agtctgttat tcgtgatgtt ttagaatata atccaaacac
    10501 gacaatgatt ataaaatcaa ccattcctgt tggttttacc aaagagatga gagagaaata
    10561 taataccaat aatattattt tctcccctga gttcttacgt gaaggtcgtg cgctttacga
    10621 taacttatac ccatctcgta ttgtgattgg tgaacaatca gaaagagcac aaaagtttgc
    10681 tgacctttta caacaaggcg cactgaaaaa agatattgat gttctgttca ctgactccac
    10741 agaagccgaa gcgattaaat tatttgctaa tacttatcta gcactccgtg ttgcttactt
    10801 taatgagcta gatagctatg ccgaatctta tggactgaac tcaagacaaa tcattgaagg
    10861 tgtctgctta gatccgcgta ttggcaatca ctataacaat ccatcattcg gttacggcgg
    10921 ttactgctta ccaaaagata ctaaacagct gttagccaac tacgactctg tccctaataa
    10981 tattattagt gcaatagtgg aagctaaccg cacacgtaaa gattttattg cagactctat
    11041 tattgcaaaa tcacccaaaa tcgtcggcgt atatcgttta attatgaaat ctggctcgga
    11101 taatttccgt tcttcttcta tccaaggcat tatgaaacgc attaaagcca aagggattga
    11161 agtggtgatt tatgaaccag agatgaaaga agagaccttc tttaattcaa aattaattcg
    11221 tgatttaaat gagtttaaag caatgtctga tgtcattatt accaatagaa tgtcatcagc
    11281 actaaacgat gttgaagaaa aagtttatac tcgtgacctc tttggtaacg attaaatcga
    11341 ttttatatgc tcaatatcgt cttatttgaa cccgaaattc caccgaatac gggtaatatt
    11401 atccgcctgt gtgctaacac aggctttcat cttcatttga tccaaccatt gggttttact
    11461 tgggatgata aacgtctacg tcgtgcaggg cttgattatc gtgaatttgc tgatattaaa
    11521 cagcaccacg attattatgc ttttttagaa agtgaaggtt taaaccctga taatgcacaa
    11581 tcaccatcag gtgcgcgtgt atttgcctta accacaaaag gcacgccagc acatagcaat
    11641 gtcagttatc aagatggaga ttatctcctg tttggccctg aaacccgtgg tttgccacct
    11701 tatgtattag ataatatgcc aacacagcag aaaatcagag tcccaatgct tgctgatagc
    11761 cgcagtatga atctgtcaaa tacggttgcg gttgtggttt ttgaggcatg gcgccaactt
    11821 ggttatcctg gtgcattact aagagactga aatgcaaaaa gcgctgatca taaataatga
    11881 ctagcgcttt ttaacttcaa ataactaaca tgttaattta ttacattgag gattgtttta
    11941 tttcagcaac aaccttcgtg atatatcgac acccttcccc tacatccttc agtactaatg
    12001 catgagctcc aatcaccgca ttatcaccaa tctctatctc accgccgata atcgatgagt
    12061 tacaaccaat ataaacgtta ttaccaaccc gaatatccgc tttgccattc ttttctttta
    12121 caccgattgt cacaccttgc attaacgtca cattctcgcc tagctgacag taattagtta
    12181 gtacgatccc tatgggatgt ggtaatgaaa ggcctttacc tatgcgcgtt cttagtgaga
    12241 tatcgttgca atatatacga ttgtttttag caccaatacg aatgcctaat tttttgataa
    12301 aacgattttt agaaaaataa aagaattggg cgacacgcca ccaaaataga taacgtgaac
    12361 gttggcagaa aaaaacacgg tgataaagtc ttatccaact aaaaggtttg ttatcaccaa
    12421 tatactcagc gtgtaaacaa gcctttagtt cagaaagtgt gacttcagtt aatgccatta
    12481 aatgccatca ccacattcaa aaccgggtac gacaccatta aagtgttgat ccatatcgag
    12541 tgatggctta tcactcgttg gtttacccac aatgcgagca ggaacaccag caactgtagt
    12601 gtgcgcagga acttcatgta aaacgactga tcccgcacca attttagcac cgcgaccaat
    12661 ctcgatattg ccgagtattt ttgcacccgc accaatcatt acaccttcac gcactttagg
    12721 atgacggtca ccacaagttt tacctgttcc acctaacgtc acagattgta aaatagaaac
    12781 atcgttctct acgatagcgg tttcaccaat tacaatgcct gttgcgtgat caagcataat
    12841 accgtggcca atacgggcag ctggatgaat atcaacacca aaggtcacag aaatctggtt
    12901 ttgcagatac accgctaacg ctttgcgatc ttgattccat aaccaatgcg cgatgcgata
    12961 agcttgtaag gcatggaaac cttttaaata aagcagtggt gtagagtatt tatcaacggc
    13021 aggatcgcgt aaacgcacag ccgaaatatc ataagctgcc gattcaatca tagaattatc
    13081 actacgatat gcttcttcaa caatctcccg aacagcaatc gcgggcattg tttgagttgc
    13141 tagcttattt gccaacatat aacttaaggc actacctaag ttttcatgtt tgagtaatgt
    13201 cgcatggaaa aaactcgcca aaattggctc gcaatctgcc aagcatcttg cttcattttt
    13261 aatatgatcc cagactcttt ttagctcttc tagcgacata ctcactctca ttctttttac
    13321 attgaagcct aagtaaatac ttaggcttct tatgtttaaa acagaaataa ataactatct
    13381 aaaatagact tagaacagat tgctgtctat ctcatcttta gtagctcggc ctaataaaga
    13441 taatgccgct tctttcgcgt ctttatgctg atataaaatt tggtagatct gctcagtaat
    13501 aggcatttca acaccaactt gttcggctaa tgcgcgaact tctttagtat tacgatagcc
    13561 ttcaactacc tgcccgattt tttcttgggc agtctccaca tcaaggcctt gacctaacat
    13621 cataccaaag cgacgattac gagattggtt atcggtacag gttaaaacta aatcacccaa
    13681 ccccgccatg cccataaagg ttgcagcatc tgcacctaac gctttaccta aacggctcat
    13741 ttcagcaagg ccacgagtaa tcaatgctgt acgcgcatta gcaccaaacc ccataccatc
    13801 agacatacct gcgccaatag caatcacgtt ttttaccgca ccacctaatt ggacaccgat
    13861 aaagtcagga tttttatata ctcggaaact tttgccacaa tggaacaact gttgtaactc
    13921 ttcagagaag gtattgtccg ttgaagccac agaaatcgca gtaggcagac ctgctgcaag
    13981 ctctttggca aaagtagggc cagataaaac tgcaagcggg atttcattac ctaatacttc
    14041 acgcgctaca tcttgcaata agcgaccagt atgagcctca agtccttttg tcgcccaaac
    14101 aacacgtgca tcctgacgta aaaagggttt gatctgctgt aatacatcac caaaaacatg
    14161 gcttgggatc acaaccagaa tattacggct agcttcaatg gctttttgca aagaagcttc
    14221 catgtataaa ctatcaggaa aggaaacatc gggcagaaag gcctgattac agcgagcttg
    14281 ctctaacgca gcaacatgtt caggatcatg gccccaaagt acaacatcat ggccattgcg
    14341 cgctaaggta atcgctaaag cggtgccgta tgaaccggca ccgataactg tcatagaagc
    14401 gttcattaag cttcttcagc cccttcatta gcggcatcag tttgttgctg ttgtaaatag
    14461 ttcataaaca acgcatcaaa gttaactggt gctaagttca gttgtgggaa agtaccacgg
    14521 ctgactaagt tagtgataca ttcacgagca taagggaaca gaacgttagg gcaatatgca
    14581 cctaagcaat gtgctaattg agtgccttca ataccttcaa cggtgaagat acctgcttgt
    14641 tgcacttcac acaggaatgc agtctcgtta tccatagttg cagttacagt aacgcgcaga
    14701 ataacttcat agacgttttc agctaaagtg ttagaagaag tatctaaatc taatttaact
    14761 tctggttgcc actctttttg gaaaacttgt ggggcattag gcgcttcaaa agagatatct
    14821 tttgtataga tacgttgaat ttgaaaagtc atctcttggt tttgctgttc tgacat
//
