LOCUS       KY710735               14250 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus penneri strain G2672 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710735
VERSION     KY710735.1
KEYWORDS    .
SOURCE      Proteus penneri
  ORGANISM  Proteus penneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14250)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="Proteus penneri"
                     /mol_type="genomic DNA"
                     /strain="G2672"
                     /serotype="O73ab"
                     /db_xref="taxon:102862"
                     /collected_by="Center for Disease Control and Prevention,
                     Atlanta, USA"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..14250
                     /note="O antigen gene cluster"
     CDS             1636..3006
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="AXZ00070.1"
                     /translation="MPRNIAVILASGTGSRFESKIPKQFVKLAGKPVIQYTIEAFENC
                     SAIDEIYVVTKEDYIDLVYEITNKNELKKISKVISGGKERYDSTWAAINATPEENCNL
                     IIHDAVRPFISEKIINDCIAALVKYNAVDVVVDAVDTIVEVDGNFISNIPDRRKLKRG
                     QTPQAFKRDTLKDAYNKFFLDPQKAASDDCGIVLKYLKEEKIYTVNGAESNFKLTHQQ
                     DLYLADNIIKDGLYTRLNADSNLIKEKIDNKVVVVIGGSSGIGEDIVTQCKKLNSRVY
                     SCSRSQNNVDVTDKKSIDLFLHEISLKENRIDFIINTTGLLIKKPLLSMSNSEITDSY
                     MINYTGVINVALASYKYLKESQGMLINFTSSSFTRGRPNYSIYSSTKAAIVNFTQAIA
                     EEWLPLGIKVNCINPERTDTPMRRSNFGIEPANSLLSAEAVAKFTLSAMSFEHTGQVF
                     SIKNDL"
     CDS             3003..4235
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00071.1"
                     /translation="MSLKKNIINLFGTQVVSYLVPLLQYPYLSRVIGTDILGLYIFSI
                     SIINISNIITTFGFDIYVAKRIAEGENSKKDVSIFIFQTSVLKIILLIIPLIIIFISK
                     FSTSYFEDNNILLLLIFSVLVNTFNLVWLFQGLEKLYIYSRIIITTKIISLLLIFLIV
                     KTKNDINLLFLITFFQYLLSVLLCFFYCYKSGYKIIPSSLQSTVSLLINSFEYFLSRL
                     GTSIYSTCGSFFLGLFSGSLHQVAIYGAAEQLYKAGVYTMTAISTPLIPYMARTKNYL
                     VFYKVTILSIIMTLIGAFIGFFYGKDIIYLIYGNNLIDAYPILCIFMITIVISIIGIQ
                     FGYPALIPINKTKIANYSVIIAGIIQLLGIITLHLLNIDINAKNITILYLASDSTMTL
                     IRFMTFIKNRNTRNKNGL"
     CDS             4225..5370
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00072.1"
                     /translation="MVFSFILYNFILFSSTLFIFLFNITKSKYIKHIFIFISFFIVFI
                     PSALRYNIGTDYRTYVEIFNKLKLYHYSYVEFGYTQLNQLIISLNLTSEYLFAIVAFL
                     TYFFIYLTYSKSNKSYIFHFLFICTGYFSSFYLLRSTISLSICIFAIALYLNNSKVFI
                     FLLLIFIASLFHKAALIYMLIPILGSMKLKKLYINLRFIILLSIFVIFIFKDQLVTFV
                     FHSKLIDILGYSDYVSSELFSKKTSYGSGLGILLRALPLIIIILLTKKLLTHDKRNSL
                     IIFTALISIISIIFSSTIEIFSRLEKMFIFSYMFGIIAILQCPTIKFRNFYCFFFILV
                     YLLIFELTIINSSSYKCEGARITPYVSILNKEDDQSLNLTRIQCEKL"
     CDS             5374..6597
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXZ00073.1"
                     /translation="MFMLKIKKLLFYIFIGYPIYIISFLIKRDHDLWLIGSKDGFRCN
                     SKYYFISHINSSLNSKIKLYWIAKDKNTFNKLTALSLPCVYKYSIKGFIFLLKAKVYI
                     TSYGTQFDLSFWTSGNVFKCYLWHGVGIKNIGYLSKKENIRRMYHSKNIFNIVKYYPY
                     REKPNLFLSTSPLMNKHFSEAFNIDKKNIFEGLYPRCEFLLSSKDDVASFINKYEDNE
                     IKGIINQLKCYDKIYFYMPTWRSNKKNIFKFLNWDIEQLNDILIKNNAFLILKLHPMS
                     EYEKSIIEYSNIMILNNNIDPYPILYYTDTLITDYSSVYFDYILLKNKELVLYPFDLE
                     EYTNEEDDLAFDYKEYTPGIYIYTFHDLLEFIDGIYSNNEDSSRKINEIRNIFWGNYE
                     SKKSIQLKEKIEKSL"
     CDS             6594..7538
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00074.1"
                     /translation="MNIKYSIIIPAFNAESYLTKIISFSKKILKKREDIEIIFVDDGS
                     IDNTSSIQNKSENIKYIYQENGGVSSARNLGLKSAIGEFILFLDADDTYDDNIFSVLD
                     VFTTDEQSNIFLLNYSINKKIQNDTLSLLNKNIFNWMEVSNMFFKREIKLHICAVCFR
                     KKHLLSNSIFFPNGYSFGEDSFFILSSIYNEQKIHYIKNSLYNYNFHHGSVVSTPLNK
                     NKIDVIELYLTLREEKYKENKVIIYFIERTYLYLLKLAIKYGIRDKETMQQLKKYKFI
                     LNSKNSSRNISFNIIKFFIYNFNHIIIDIIYLKSRARK"
     CDS             7535..8350
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00075.1"
                     /translation="MKFSVLVSLYFKENPINLDSALRSLWSEQKLLPTEIIIIKDGPL
                     TLELDNILDKWKDLLSNNLKIIALKNNVGLGFALNEGLKHCTYDWVFRMDTDDICLPN
                     RFEKQIDFIKHNPDISLLGSSTEEFDENMENSFGYRINPTEHNDIVNYAKKRNPINHM
                     TVAFKKEAVLAVGGYQHHLFMEDYNLWIRMISSGYKVANLPDILVKVRAGNSMVTRRK
                     GLKYIKSEYKLANLKIKTNLDNSFSAYSIFLLRSIPRMLPTSFLSKIYKMLRK"
     CDS             8364..9311
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXZ00076.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSEKYPEKWIFG
                     DVTKPDTLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWYKKDNTNTLIVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATKIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHTHIKVPYAIGLLGGYCFDVLSKVTGKEFPVSSIR
                     IKKFCARTQFKSNSITNTDFKAPVTLQQGIANTVKNEFL"
     CDS             9543..10709
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00077.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             10721..11224
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXZ00078.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPSGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11295..11852)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXZ00079.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWIRLYHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFIKKLGIRIGAKNNRIYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKNGKADIRVGNNVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIKQSSM"
     CDS             complement(11852..12685)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00080.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(12764..13780)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00081.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVETAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(13780..14250)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4946 a   2375 c   2249 g   4680 t
ORIGIN
        1 atgctagaac aacatatcga agctgaatta gctcaagatc cggctaacga tctcctttgg
       61 tggcgtcgat taattcgagc cattgataaa tgggctcccc ctggccaacg tttaattatt
      121 gtcacgagtg aaggccggat tatcggtgct caacgtaatg agatgcaagt tgtcagaaac
      181 tttattggtc aatctgataa cgccgatcac cccaaaaaga aaaaatatgg tcgctctgag
      241 atgctagggc ctttttctgt aagggatggt gaagatcatt accaacttta tctcgtgcgc
      301 ccttcaagta gccctcaatc tgactttatc aacttattat ttgataaacc attgttgcta
      361 ctcatattca ccatgcttat cagcacacca ttattagtat ggctttcttg gagcttagca
      421 aaacccgcaa gacagcttaa aaatgcggct gacgatgtgg ctaaaggtaa cctacgccct
      481 caccctgaac tagaaactgg ccctcaagag tttttagccg caggcacgag ttttaatcag
      541 atgataagtg ctcttgaacg tatggtagag gcgcaacaac gtctgatctc tgatatttct
      601 catgaactac gtaccccatt aacacgctta caattagcca gtgcattgtt acgtcgtcgt
      661 agtggagaga gcaaggagct agagcgtatt gaaactgaga ctcaacgcct tgatggtatg
      721 attaatgact tactagtact ttcccgtaat cagtataaaa acgagttatt acgcgaaaca
      781 gtaaaagcca acgaattgtg ggatgacatt ttagataacg ctaaatttga ggccgaacaa
      841 agtaataaga cactgcaagt cacaacacct ccaggagcct ggcctattta ctgcaatcca
      901 tattctctgg cgagtgcatt tgagaatata gtccgtaatg ctctgcgtta ttcaaattct
      961 cgcattgaag tcgcgtttac tgaacagaac caaggtatca ccattattgt tgatgatgat
     1021 ggccctggcg ttagccctga agatagagag catatcttcc gaccattcta tcgcactgac
     1081 gaagctagag atcgtgaatc cggtggtact ggcttaggat tagcgattgt tgaaactgcc
     1141 gtcagccaac acagaggcca tgttaaagcc gatgacagtc cattaggtgg gttgagggtt
     1201 gagatttggt tgccgagagc gggtgcaagt aacaaatcgc caacggctta atagagtaat
     1261 taaactgttg tatcaaaata tcctgccatc tattgaagaa taggtggcat attctttttg
     1321 ttcgcattta ttgatgcaat tcagaatgat ctggctgaat cgatcgctat tccgatattt
     1381 atatatctac aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt
     1441 ctactctcag tgcggatttt cgcatattgt taaagtatgc ttatataata aaattcttat
     1501 attacatata gataagggtt tttcgagata aattttggcg ctctgggggc cgtaaaccca
     1561 gtgcggtagc tttgtaataa atatattttt cctccctaca ttaataatta atttgttctt
     1621 attaaaggat aagtaatgcc tagaaatatt gcagtgatct tagcttcagg aacaggttct
     1681 cgcttcgaat ccaaaattcc aaagcagttt gtaaaattag cagggaagcc cgtcattcaa
     1741 tatactatcg aagcttttga gaattgttca gctatagatg aaatttatgt tgtcactaaa
     1801 gaagattata tcgacttggt ttatgaaatt actaataaaa acgaattaaa gaaaatatct
     1861 aaagtaattt caggtggtaa agaacgttat gattcaacat gggctgcaat taacgctaca
     1921 cctgaagaaa attgcaattt aattattcat gatgcagtta gaccatttat ttcagaaaaa
     1981 attattaatg actgtatagc ggccttagta aaatataacg ctgtagacgt tgttgtagat
     2041 gctgtagaca caattgttga agttgatggt aattttattt ctaatattcc tgacagaaga
     2101 aaattaaaac gcggacaaac tccccaagct tttaaaagag atacattaaa agatgcctac
     2161 aataaattct ttttagatcc acaaaaagca gcatcagatg attgtggcat tgtactaaaa
     2221 tacttaaaag aagaaaaaat atatacagtc aatggcgcgg agtctaactt caagttaaca
     2281 catcaacaag atttatattt agctgataat attataaaag atggcttata tactcgatta
     2341 aatgctgata gtaacttaat aaaagagaaa atagacaata aagttgtagt cgttattggg
     2401 ggtagtagtg gtattgggga agatattgtc actcaatgta aaaaattaaa tagcagagtt
     2461 tactcttgta gccgaagtca aaataacgta gatgtaacag ataaaaaaag tattgattta
     2521 tttctccatg aaatttctct taaagaaaat agaatagact ttattattaa tacgacaggt
     2581 ctattaataa aaaaacctct tttatccatg agcaattctg agattactga tagctatatg
     2641 ataaattaca ctggtgtaat taatgtagca ctcgcatctt ataaatatct aaaagaatct
     2701 caaggtatgc tcattaactt cacatctagt tcatttacta gaggaagacc taattatagt
     2761 atttattcat caacaaaagc tgcaattgtt aattttactc aagcaattgc agaagaatgg
     2821 ctgccattag gtattaaagt aaactgcatt aatcctgaaa gaacagatac gccgatgaga
     2881 cgtagtaatt ttgggattga gccagctaat tcattactta gtgcagaggc tgttgccaaa
     2941 tttacgctat ctgcgatgtc atttgaacat actggacaag tttttagtat aaagaatgat
     3001 ttatgagcct taaaaaaaat ataattaatt tatttggaac tcaagtagtc agctacttag
     3061 ttccattatt acaatatcca tatctaagta gagtcattgg tactgatatt ctgggcttat
     3121 atattttctc aatatcaatc attaatatat ctaatattat aacaacattt ggcttcgata
     3181 tttatgtagc caaacgaata gctgaaggag aaaatagcaa aaaggatgta agtattttta
     3241 tatttcagac gtcagtatta aaaataattt tactaattat tcctttgatt ataattttca
     3301 tatcaaagtt tagcacatca tattttgaag ataataatat attattattg ctaatatttt
     3361 ctgtactagt caatacattc aatttagttt ggttatttca aggattagag aaactctata
     3421 tatatagccg aattataata acaactaaaa taatatcttt gctattaata tttttaattg
     3481 ttaaaactaa aaatgatatt aatttattat ttttaattac tttttttcaa tatctattat
     3541 ctgtactgtt gtgctttttt tattgttata aatctggtta taaaataatt ccatcttctt
     3601 tacaaagcac agttagttta cttattaatt catttgaata ttttttatcc cgcctaggca
     3661 cttcaattta ttcaacatgt ggtagctttt tcttaggttt attttctggt tcattgcatc
     3721 aagtagccat ttatggtgca gccgaacagc tatataaagc tggcgtatac actatgacag
     3781 ctatttccac ccctcttatt ccatatatgg caagaacaaa aaattactta gttttttaca
     3841 aagtaacaat attatccatt ataatgactt tgattggtgc atttattgga ttcttttatg
     3901 gtaaagatat tatatatcta atatatggta ataatttaat tgatgcatat ccaatactct
     3961 gtatttttat gataacaatt gttattagta ttataggtat tcaatttgga taccctgcat
     4021 taatacctat aaacaaaact aagatagcta actatagtgt aataatagca ggaataatac
     4081 agctacttgg aattatcaca ttacacttat taaatataga tattaatgcc aagaatataa
     4141 ctatattata tttagctagt gatagcacca tgactttaat aagatttatg acttttatta
     4201 aaaatagaaa tactaggaat aaaaatggtc tttagcttta tattatataa tttcattcta
     4261 ttttcaagta cactttttat ttttttattt aacataacaa aatcaaaata tattaaacat
     4321 atatttatat ttatatcttt ctttatagta tttataccat cagcattacg ttataatata
     4381 gggactgatt atagaactta tgttgaaata ttcaacaaat taaaacttta ccattattct
     4441 tatgtggagt ttggatacac tcaattaaat caattaatta tatcccttaa cttaacttca
     4501 gaatatttat ttgcgattgt agcattttta acatattttt tcatatatct aacatattct
     4561 aagtcaaata aaagctatat atttcatttt ttatttattt gtacaggata tttttctagt
     4621 ttttatctat tacgctcaac tatttcatta tcaatttgta tctttgcaat agccttatac
     4681 ttaaataact ctaaagtctt tatattttta ttattaattt ttatagcctc actattccat
     4741 aaagcagcac taatatatat gcttattcca atattaggct caatgaaatt gaaaaaactt
     4801 tatataaatc ttcggtttat aattttactt tctatttttg taatatttat ctttaaagac
     4861 caattagtta cctttgtatt tcatagtaaa ttaatagata ttctaggata ttcagattat
     4921 gtttcatcag aactattttc taaaaaaaca tcatatggaa gtggtctagg aattttatta
     4981 agagctcttc ctcttatcat aataatcctt cttacaaaaa aattattaac ccatgataaa
     5041 agaaatagtt taataatttt tacagcatta atatctatta tatctataat tttttcatcg
     5101 actattgaaa ttttttctcg tcttgaaaaa atgttcatat tttcttacat gtttggtatt
     5161 atagctatat tacaatgccc aacaatcaaa tttagaaatt tttattgctt ctttttcata
     5221 ttagtatacc tccttatatt tgaattaact attattaatt catcatctta taaatgtgag
     5281 ggggctagaa taactcccta cgtaagtata ttaaataaag aagatgatca gagcctaaat
     5341 ttaactagaa tacaatgtga gaaattataa tttatgttta tgcttaaaat aaaaaaatta
     5401 ttattttata tttttattgg ttatcctatc tatattattt cctttttaat aaaaagggat
     5461 catgacctat ggctaatagg ttctaaagat ggatttagat gcaactctaa gtattatttt
     5521 atttcacata taaactcatc attaaatagt aaaataaaac tatattggat agccaaagat
     5581 aaaaacacgt ttaataaact cactgctcta tcgttacctt gtgtttataa atactcaatc
     5641 aaaggattta tttttttatt aaaagctaaa gtatatatta cttcatatgg tacacaattt
     5701 gatttatcgt tctggacttc tggaaatgtt tttaaatgtt atctttggca cggagtaggg
     5761 ataaaaaata ttggttatct atctaaaaaa gaaaacataa gaagaatgta tcactcaaaa
     5821 aatattttta atatagtcaa atattatccc tatagagaaa aacctaatct ctttctctcc
     5881 acctccccat taatgaataa acatttcagc gaagcattta atatagacaa aaagaatata
     5941 tttgaaggat tatatcctag gtgtgagttt ttgcttagct ctaaagatga tgttgctagt
     6001 tttattaata aatatgaaga taatgaaatt aagggcatta taaatcaatt aaaatgttat
     6061 gataaaattt acttttatat gccaacttgg cgtagtaata aaaaaaatat atttaaattt
     6121 ttgaattggg atatagaaca actcaatgac attttaataa aaaacaatgc ttttcttata
     6181 ttgaaactac atccaatgtc ggaatacgaa aaatctataa tagaatatag taatataatg
     6241 atacttaaca ataatataga tccatatcct atattatatt atacagatac tttaattaca
     6301 gattattcat ctgtatattt tgactacatc ttattaaaaa ataaagaact tgttttatat
     6361 cctttcgacc tagaggagta tactaatgaa gaagatgatt tagcatttga ctacaaagaa
     6421 tataccccgg gtatatacat atataccttt catgacttat tagaatttat agatggcatt
     6481 tatagtaata atgaagattc atcaagaaaa ataaacgaaa taagaaatat attttgggga
     6541 aattatgagt ctaaaaaatc aatacaatta aaagaaaaaa tagaaaaatc attatgaata
     6601 taaagtattc cattataata ccagcattta atgcagaaag ttatttaaca aaaataatat
     6661 cattttcaaa aaagatttta aaaaaaagag aagatattga aataattttt gtggatgatg
     6721 gctcaataga taatacttca tcaattcaaa ataaaagtga gaatataaaa tatatttatc
     6781 aagaaaatgg tggggtatct agtgccagaa atttaggatt aaagtcagct ataggagaat
     6841 ttattttatt tctagatgct gatgatacat atgatgataa tatattttca gtattagatg
     6901 tttttactac agatgaacaa tcaaatattt ttttattaaa ttattctata aataagaaaa
     6961 tacaaaatga tactctctca cttctaaata aaaatatatt taattggatg gaagtgtcaa
     7021 atatgttttt taaaagagaa ataaaactgc atatttgcgc agtgtgtttt agaaaaaaac
     7081 atctattaag taactctatc ttttttccta acggatattc ttttggtgaa gatagttttt
     7141 ttatattatc tagcatttat aatgaacaga aaattcatta tataaagaat tcactttata
     7201 actataattt tcaccatggc agtgttgtta gcacgccttt aaataaaaac aaaatagatg
     7261 ttatcgagct ttatttaacg ttaagagaag agaaatataa agaaaataaa gtcattattt
     7321 actttataga aagaacatat ttatatttat taaaactagc tataaaatat ggaattcgtg
     7381 ataaagagac catgcaacaa ctgaaaaaat ataaatttat acttaattca aaaaactctt
     7441 ctagaaatat ttcatttaac attattaagt tttttatata taactttaat catattatta
     7501 ttgatataat atatttaaaa tcaagagcta gaaaatgaaa ttctctgtat tagtttcact
     7561 atattttaaa gaaaacccta ttaatttaga ctccgcactt agaagcctgt ggagtgaaca
     7621 aaaattatta ccaacagaaa taataataat aaaagatggt cctttaacac tagagttaga
     7681 taacatatta gacaaatgga aggatttatt atctaataat ttaaaaatta tagcattaaa
     7741 aaataatgta ggtcttggat ttgcattaaa tgaaggttta aagcattgta catatgattg
     7801 ggtttttaga atggacactg atgatatatg cttaccaaac cgttttgaaa aacaaattga
     7861 ttttataaaa cataatccag acatttcgct tttaggttca tcaacagaag aatttgatga
     7921 aaatatggaa aactcttttg gctatcgtat taatccaaca gaacataacg atatagtcaa
     7981 ttatgcaaaa aaaagaaatc ccattaatca catgaccgtt gcttttaaaa aagaagctgt
     8041 tttagccgtc ggcgggtatc aacatcattt atttatggaa gattataact tatggattag
     8101 aatgatttct tctggatata aagtagctaa tttacctgat attcttgtca aagttagagc
     8161 aggtaactct atggttacac gaagaaaagg gctcaaatat ataaaaagcg aatataaatt
     8221 agctaattta aaaataaaaa cgaatttaga taattctttt tctgcatatt ctatttttct
     8281 actgagaagt attccaagaa tgctacctac atctttttta tctaaaattt ataaaatgtt
     8341 aagaaaataa ccaaggtatt tctatgctaa caatcatcgg tggttcaggt tttattggca
     8401 cacgcctttc agatcaacta actaaagaaa atattgaatt taaaattgta gatattgcta
     8461 aaagtgaaaa atatcctgaa aaatggatat ttggtgatgt aacaaaacca gatacattac
     8521 ttgaaccatt aaaaggctct gatgttatta tcaacttagc ggcacaacat aaagataatg
     8581 ttcatcctat tagtttatat tatgaagtta atgtagatgg agctaaaaat gtttgtgatg
     8641 ttgctgaaca actaaatatt aaacatatcg tatttacttc atctgttgct gtatatggat
     8701 ttgttgaaaa agaaacgggt gaagatggtg agtttcatcc atttaatgat tatggaaaat
     8761 caaaacttga agctgaacat aagtatgaag cttggtataa aaaagataat acaaatacat
     8821 taatcgttat caggccaacc gttgtatttg gtgaaggcaa tagagggaac gtatataacc
     8881 tattccgcca aatagcatcg ggtaaatttt taatggtggg ttcaggtaat aataaaaaat
     8941 ctatggctta tgtagaaaac atcgctgctt tcttaaaatt tgctactaaa atagaatctg
     9001 gtcatcatat atttaattat attgataaac ctgattttac tatgaatgaa ttgacagata
     9061 tcatttatac agctctagat aaacgacata cccacattaa agttccttat gctattggat
     9121 tacttggtgg atattgtttt gatgttcttt ctaaagttac aggtaaagaa ttccctgtaa
     9181 gcagtattcg aatcaaaaaa ttctgtgccc gcactcaatt taaatcaaat agtattacta
     9241 atactgattt taaagcgcca gttactcttc aacaaggtat tgctaacaca gttaaaaatg
     9301 aatttttata atagattaat tttaaatagt ctttgtatta acaaataatt aaaatacacc
     9361 ctacacttct ctacttattt tctaataaaa aattaatttt acattaattt aataagtata
     9421 tgatccttat gattgctaaa tatactatac ctttactaaa gagtgagaat taacactaaa
     9481 atatctattt ttattctagt atagtgaaat tataaataca tatactttag ataagagaaa
     9541 ccatgaaaat caccatctcc ggtaccggct atgtcggtct atctaacggg atactgcttt
     9601 cccaacacaa caaagttgtt gcgttagata tcaataaaga aaaagttgaa ttacttaaca
     9661 aaaaaatctc gccaattaca gacaaagaaa ttgaagagtt tttagcaacc aaagagctcg
     9721 actttacagc aacattagat aaagaattcg cttataaaga tgctgaattc gttatcattg
     9781 ctacaccaac cgattatgat cctaaaacga actatttcaa cacctcttct gtagagtctg
     9841 ttattcgtga tgttttagaa tataatccaa acacgacaat gattataaaa tcaaccattc
     9901 ctgttggttt taccaaagag atgagagaga aatataatac caataatatt attttctccc
     9961 ctgagttctt acgtgaaggt cgtgcgcttt acgataactt atacccatct cgtattgtga
    10021 ttggtgaaca atcagaaaga gcacaaaagt ttgctgacct tttacaacaa ggcgcactga
    10081 aaaaagatat tgatgttctg ttcactgact ccacagaagc cgaagcgatt aaattatttg
    10141 ctaatactta tctagcactc cgtgttgctt actttaatga gctagatagc tatgccgaat
    10201 cttatggcct aaatgcaaga caaatcattg aaggtgtctg cttagatccg cgtattggca
    10261 atcactataa caatccatca ttcggttacg gcggttactg cttaccaaaa gataccaagc
    10321 aactgttagc caactacgac tctgtcccta ataatattat tagtgccatc gtggaagcaa
    10381 accgcacacg taaagatttt attgcagact ctattattgc aaaatcaccc aaaatcgtcg
    10441 gcgtatatcg tttaattatg aaatctggct cggataattt ccgttcttct tctatccaag
    10501 gcattatgaa acgcattaaa gccaaaggga ttgaagtggt gatttatgaa ccagagatga
    10561 aagaagagac cttctttaat tcaaaattaa ttcgtgattt aaatgagttt aaagcaatgt
    10621 ctgatgtcat tattaccaat agaatgtcat cagcactaaa cgatgttgaa gaaaaagttt
    10681 atactcgtga cctctttggt aacgattaaa tcgattttat atgctcaata tcgtcttatt
    10741 tgaacccgaa attccaccga atacgggtaa tattatccgc ctgtgtgcca acacaggctt
    10801 tcatctccat ttgatccaac cattgggttt tacttgggat gataaacgtc tacgtcgtgc
    10861 aggacttgat tatcgtgaat ttgctgatat taaacagcac cacgattatt atgctttttt
    10921 agaaagtgaa ggtttaaacc ctgataatgc acaatcacca tcaggtgcgc gtgtatttgc
    10981 cttaaccaca aaaggcacgc cagcacatag caatgtcagt tatcaagatg gagattatct
    11041 cctgtttggc cctgaaaccc gtggtttgcc accttatgta ttagataata tgccaacaca
    11101 gcagaaaatc agagtcccaa tgcttgctga tagccgcagt atgaatctgt caaatacggt
    11161 tgcggttgtg gtttttgagg catggcgcca acttggttat cctggtgcat tactaagaga
    11221 ctgaaatgca aaaagcgctg atcataaata atgactagcg ctttttaact tcaaataact
    11281 aacatgttaa tttattacat tgaggattgt tttatttcag caacaacctt cgtgatatat
    11341 cgacaccctt cccctacatc cttcagtact aatgcatgag ctccaatcac cgcattatca
    11401 ccaatctcta tctcaccgcc gataatcgat gagttacaac caatataaac gttattacca
    11461 acccgaatat ccgctttgcc attcttttct tttacaccga ttgtcacacc ttgcattaac
    11521 gtcacattct cgcctagctg acagtaatta gttagtacga tccctatggg atgtggtaat
    11581 gaaaggcctt tacctatgcg cgttcttagt gagatatcgt tgcaatatat acgattgttt
    11641 ttagcaccaa tacgaatgcc taattttttg ataaaacgat ttttagaaaa ataaaagaat
    11701 tgggcgacac gccaccaaaa tagataacgt gaacgttggc agaaaaaaac acggtgataa
    11761 agtcttatcc aactaaaagg tttgttatca ccaatatact cagcgtgtaa acaagccttt
    11821 agttcagaaa gtgtgacttc agttaatgcc attaaatgcc atcaccacat tcaaaaccgg
    11881 gtacgacacc attaaagtgt tgatccatat cgagtgatgg cttatcactc gttggtttac
    11941 ccacaatgcg agcaggaaca ccagcaactg tagtgtgcgc aggaacttca tgtaaaacga
    12001 ctgatcccgc accaatttta gcaccgcgac caatctcgat attgccgagt atttttgcac
    12061 ccgcaccaat cattacacct tcacgcactt taggatgacg gtcaccacaa gttttacctg
    12121 ttccacctaa cgtcacagat tgtaaaatag aaacatcgtt ctctacgata gcggtttcac
    12181 caattacaat gcctgttgcg tgatcaagca taataccgtg gccaatacgg gcagctggat
    12241 gaatatcaac accaaaggtc acagaaatct ggttttgcag atacaccgct aacgctttgc
    12301 gatcttgatt ccataaccaa tgcgcgatgc gataagcttg taaggcatgg aaacctttta
    12361 aataaagcag tggtgtagag tatttatcaa cggcaggatc gcgtaaacgc acagccgaaa
    12421 tatcataagc tgccgattca atcatagaat tatcactacg atatgcttct tcaacaatct
    12481 cccgaacagc aatcgcgggc attgtttgag ttgctagctt atttgccaac atataactta
    12541 aggcactacc taagttttca tgtttgagta atgtcgcatg gaaaaaactc gccaaaattg
    12601 gctcgcaatc tgccaagcat cttgcttcat ttttaatatg atcccagact ctttttagct
    12661 cttctagcga catactcact ctcattcttt ttacattgaa gcctaagtaa atacttaggc
    12721 ttcttatgtt taaaacagaa ataaataact atctaaaata gacttagaac agattgctgt
    12781 ctatctcatc tttagtagct cggcctaata aagataatgc cgcttctttc gcgtctttat
    12841 gctgatataa aatttggtag atctgctcag taataggcat ttcaacacca acttgttcgg
    12901 ctaatgcgcg aacttcttta gtattacgat agccttcaac tacctgcccg attttttctt
    12961 gggcagtctc cacatcaagg ccttgaccta acatcatacc aaagcgacga ttacgagatt
    13021 ggttatcggt acaggttaaa actaaatcac ccaaccccgc catgcccata aaggttgcag
    13081 catctgcacc taacgcttta cctaaacggc tcatttcagc aaggccacga gtaatcaatg
    13141 ctgtacgcgc attagcacca aaccccatac catcagacat acctgcgcca atagcaatca
    13201 cgttttttac cgcaccacct aattggacac cgataaagtc aggattttta tatactcgga
    13261 aacttttgcc acaatggaac aactgttgta actcttcaga gaaggtattg tccgttgaag
    13321 ccacagaaat cgcagtaggc agacctgctg caagctcttt ggcaaaagta gggccagata
    13381 aaactgcaag cgggatttca ttacctaata cttcacgcgc tacatcttgc aataagcgac
    13441 cagtatgagc ctcaagtcct tttgtcgccc aaacaacacg tgcatcctga cgtaaaaagg
    13501 gtttgatctg ctgtaataca tcaccaaaaa catggcttgg gatcacaacc agaatattac
    13561 ggctagcttc aatggctttt tgcaaagaag cttccatgta taaactatca ggaaaggaaa
    13621 catcgggcag aaaggcctga ttacagcgag cttgctctaa cgcagcaaca tgttcaggat
    13681 catggcccca aagtacaaca tcatggccat tgcgcgctaa ggtaatcgct aaagcggtgc
    13741 cgtatgaacc ggcaccgata actgtcatag aagcgttcat taagcttctt cagccccttc
    13801 attagcggca tcagtttgtt gctgttgtaa atagttcata aacaacgcat caaagttaac
    13861 tggtgctaag ttcagttgtg ggaaagtacc acggctgact aagttagtga tacattcacg
    13921 agcataaggg aacagaacgt tagggcaata tgcacctaag caatgtgcta attgagtgcc
    13981 ttcaatacct tcaacggtga agatacctgc ttgttgcact tcacacagga atgcagtctc
    14041 gttatccata gttgcagtta cagtaacgcg cagaataact tcatagacgt tttcagctaa
    14101 agtgttagaa gaagtatcta aatctaattt aacttctggt tgccactctt tttggaaaac
    14161 ttgtggggca ttaggcgctt caaaagagat atcttttgta tagatacgtt gaatttgaaa
    14221 agtcatctct tggttttgct gttctgacat
//
