LOCUS       KY710736               14880 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain CCUG 10705 O antigen gene cluster,
            complete sequence.
ACCESSION   KY710736
VERSION     KY710736.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14880)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14880
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="CCUG 10705"
                     /serotype="O74"
                     /culture_collection="CCUG:10705"
                     /db_xref="taxon:584"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..14880
                     /note="O antigen gene cluster"
     CDS             1586..2860
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00082.1"
                     /translation="MFLLKNTSYTLVGNVIFALSQWLILIILNKFGNSTDVGIFTYSL
                     AITTPIFLLSQCGLRSYLVVDIKKAFHFKIYLNLRILLSIIALSIIFFICFFIENDKT
                     RYSVLLVSLFKLFDSIFDIYYGSYQRNFLMKRIAIXXXXXXXXXXXXXXXXXXXXXXX
                     XXXXXXXGAILNIIFFLIIYLYSKNLIYSLFGYSISAILCYYFYDFRYKEDTLSYINE
                     FKSYNLLLLSALPLGLTAVFISLNSSIPRIILENKTTLTEIGAFSSFFYLLQIAQIII
                     QSLCQTYSPHISRLHIEKDKKNLQKSIINLLTLTLFISISYIASSLILKDLIFSLLFN
                     KDFYAFHKTYDYITYYSPLLFFMIALGNISTALKKIKLQPYIFFFSTLLILPLSIICI
                     SKYNLNGAILAISISSLITICLIISYINSKIK"
     CDS             2863..3912
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00083.1"
                     /translation="MNQKKKVLHIISSLNIGGTQVLLRDLLKTTSFSNYQHYFLVEES
                     SGFLDNEIIKLGGEIIRVKKIKKTTFIYYIYNLIRIIKIKKIDIVHSHVSYFSGINIF
                     ISFLYNVKIRISHAHSMSKKNNKYTSLMRLLINKFSTQKIACSHETGISIFGSKTDFT
                     IIKNGIFLNKRNIKQENNISPLIIGHIARLVPEKNQKFLIDLISKKNNSELHIIGDGP
                     LKNELIEYSQKIGVSDRVYFHGVSRYPFKDIGKYKCNVMIYPSIREGFGISIIESQYY
                     GFPTVISNNVPKEAIVIPSLVKTISLENSDIDIDTFLTKNKKISQTIIDEYFINKECD
                     IKYTANKIGSIYESK"
     CDS             3899..4438
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="hypothetical protein"
                     /protein_id="AXZ00084.1"
                     /translation="MKVNNIILKLYKISFTFIQMIIENIKINPFRSHIHGYISNINGL
                     CIIKSGLRNKRNLNISINKGSLIIKENVFFNNNCSINVRKSIKIGKNTIFGEGVKLYD
                     HDHDYSKGINHLRSSFITAPIVIGDNVWIGSNTIILKGVTIGNNCIIAAGSIITKNVS
                     DNTIIIQKRTSEYHLTSEN"
     CDS             4449..5873
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00085.1"
                     /translation="MRNLIISILINTLLFGFFIILNLNSLDYYIIPLYVTSIIIVYYI
                     IPGFIYKDFFSPTFIISLTIYHLFIIAPLLHINFNFYYMFFFKKYDVWKSYYNNWASL
                     NLIGVILFYFSLHLFNKIKPLIKSKKKNIYDIRTAPFYFITILFLLFSLVIQLYVYYK
                     FGGVLGYIKAKETIGPDAFKGLGKFAIIAESFPIIFMFLAVFYYRNKKLPWVNIFILM
                     FIFILLKILFGGLSGSRSNTIWGLFWCLALIHFFIRKLNIKFITLCTVFIISFMLVYG
                     VYKKYKTDFFYAIENHGISSNNYYNTATDSILLKSILTDFSRADIQSFAIYSQSEGYY
                     NKLRYGETYISSLLLFIPNSLLDLERKGSPGAELLHQQYDKSTSRVLGLQGEFIINFP
                     YYFYPLVFIPIAFFISRLQMIYKNRTEKQLDLLILPFFINIGIILFLSDSDNIAYFIL
                     KNGFIPMCIYILLNLITYKKKLYD"
     CDS             5866..7029
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00086.1"
                     /translation="MTKKICFLDSYHTGLNGAPKSMLSLAKGLYEKSFNVTILTTKND
                     LLLQKASNLNLNTDFINTPQVLLKSRKNKSIFFKISYIISLFYFWFLLLISKNQYFKR
                     YDVICINDIRSFLLFLPLLVIYKKKLIWYIRINERIKLITKTALILSKKIILISKDSF
                     SIFNENEIKKYSNKFSIINTGFNFPSLKEKNISLHNLRDDLQIEKNDIVFISIGSICE
                     RKNQLSIINTFKKLHIDNKKLILIGSPTSDTDKLYEALLDKEILKYGLNRNIIKIPYS
                     DNIFDYLKLSHIFLFASKKEGLPRVLIEALSTGCYLCSSNVDGVNDIIDNDKIGLKTD
                     ISSYDIYFEDSFLNLINNALCHYSKDKEFRIKYVREKFNYTNFINNFIKTILS"
     CDS             7061..7939
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00087.1"
                     /translation="MKHLKNTFRDILRFFLGKKIYTYIRFILVHKYIPNFKQPSSFSE
                     KIIFRKLYDNPKKYSSFVDKYTVRKYIESKIGPDYLIPLLKAKNFITPSDFDSLPNEF
                     VIKTSNGGGGENVLIVNNKNNLNLDEICDRFNNYLKVKIGNKIDEYFYDIEQPMILFE
                     KLLRDDFYGYPPDFKIHIFNGIKNKKIIQIDESRFNHHKRSLYNEKKEKLPFNIQPKY
                     EAVSDNFSWPVNWNEMLDISMKLADDFKYVRVDLYNVNGKIFFGELTFCHGSGWEPIH
                     PKKYDFLLGKYWHEYE"
     CDS             7926..9050
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /gene="gt4"
                     /protein_id="AXZ00088.1"
                     /translation="MNMNNKKIAITSNTSWYLYNFRKNTIISLIEAGFKVYTVSPIDS
                     YSNKLKELGAEHINIKIDSASKNPLIDLYTLISFYKIFKQIRCNAVLNFTPKNNIYGT
                     LAAKLNKIKVINNIAGLGTLFVNDTISCKLARNLYRYSQKYADKIFFQNNDDLSLFLE
                     KKYVDNVQVDRLPGSGVDLSRFTLSLSEYKHKFRFLLVARMLYEKGITFYVEAARILK
                     ERYGDKVEFCLLGFVGVNNPSAITHKQMNNWVAEGIINYLGTSDTVENELAQADCIVL
                     PSFYREGVPKTLLEAGAMGKPIITTDNVGCRETVTHGFNGYICQPKSVSSLVDAMDRF
                     INLPYEKKLKMGQNSRQKIETEFDERIVIKKYLDALKEIM"
     CDS             9065..10231
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00089.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             10257..11267
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXZ00090.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKITQLFEAEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTTQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             11271..11774
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXZ00091.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11904..12458)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXZ00092.1"
                     /translation="MQAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRIAQYFYFSPHRFLHKLGLRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYITKVIADIKQV"
     CDS             complement(12461..13294)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00093.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13391..14407)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00094.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPNHVAALEQA
                     RCNQAFLPDVSFPDSLYVEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSLL"
     gene            complement(14407..14880)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5150 a   2405 c   2326 g   4911 t
ORIGIN
        1 atgctagagc aacatataga agccgaacta gcgcaagatc cggctaacga tctgctatgg
       61 tggcggcgat taattcgcgc cattgataaa tgggcgccgc caggtcaacg tcttattatt
      121 gttaccagtg aaggacgcat tattggtgct caacgtaatg agatacaagt tgtcagaaac
      181 tttatgggac aatctgataa cgcagaccac ccgaaaaaga aaaaatatgg ccgctctgag
      241 atgttaggcc ctttttccat tcgagatggt gaagatcact atcaacttta cttagtgcgc
      301 ccatcaagta gtccacaatc tgactttatc aatttattat ttgataaacc gcttttatta
      361 ctgatattca ccatgcttat cagtacaccg ctactggttt ggctctcttg gagcttggca
      421 aaaccggcac gaaagcttaa aaatgcggcg gatgatgtag caaaaggtaa tctgcgccct
      481 caccctgagc tggaaaccgg cccacaagaa tttttagcgg caggcaccag ttttaatcag
      541 atgatcagtg cattagagcg tatggttgaa gcgcaacagc gattgatttc tgatatctcc
      601 catgaattgc gcacaccgtt aactcgttta cagctcgcca gtgcattgct acgccgtcgt
      661 agcggggaaa gtaaagagct agagcgtatt gaaacggaaa cacagcggct agatagcatg
      721 atcaatgact tattagtgct ttctcgcaac cagtataaaa atgagttatt acgtgaaacc
      781 gtaaaagcga atgagctttg gaatgatatt ttagataatg cgaaatttga ggctgaacag
      841 agcaataaaa ccctaacagt aactgcccca ccaggcccat ggacgattta ttgcaatcct
      901 tattcattag cgagtgcctt tgaaaatatc gttcgcaatg ccttgcgtta ttcacattct
      961 cgtattgaag tggcctttac cgagcaaaat cagggtatca ctattattgt tgatgatgat
     1021 ggcccagggg ttagcccaga agatcgcgag catattttcc gaccttttta tcgtacggac
     1081 gaagcacgtg atagagaatc cggcggtaca gggctgggac tagcaattgt tgaaaccgca
     1141 attagtcaac atcgaggcca tgtaaaagct gacgatagcc cattaggggg attaagagtg
     1201 gagatttggt tgcctagggg gagtgtggta aataaatcac aacacgctta attcagtaat
     1261 aaaattacta tattaaatta ttctgccatc tattgaagaa taggtggcat attcttttta
     1321 ttcacatctt tgtagatatt tcagaatgat cttgctaaat cagtcgatat tccgatactt
     1381 atatatacct aatcaatgtt agaatagcca tcaattgctt gacccgctgg tttgaagagt
     1441 ctaatctcaa agcggttttg catactgtta aagtatgctt atataacaaa actcttatat
     1501 tacagataga taagagattt tcgagaaaat tttggcgctc tgggggccgt aaacccagtg
     1561 cggtagctat gattataaat attttatgtt tttattgaaa aatacgtctt atacattagt
     1621 aggtaatgta atatttgcat tatctcaatg gttaattctt ataatattaa ataaatttgg
     1681 taatagcaca gatgtaggta tatttactta ttctttagca attactaccc ccattttttt
     1741 attaagtcaa tgtggacttc gttcatattt agtagttgat ataaaaaaag cttttcattt
     1801 taaaatttat ttaaacttaa gaatattact ttctattata gctctaagta ttatattttt
     1861 tatatgtttt ttcattgaaa atgataaaac cagatattct gttttattag tcagtctgtt
     1921 taaattattt gattctattt ttgatatata ttatggttct tatcaaagaa attttttaat
     1981 gaaaagaatc gcaatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggggag ccatattaaa
     2101 tataatattc tttttaataa tatatttata tagtaaaaat ctgatatact cattatttgg
     2161 ttattctatc agcgcaatat tatgttatta tttttatgat tttagatata aagaagatac
     2221 gttatcatat ataaatgaat ttaaatctta taatttacta ttactatcag ctcttccttt
     2281 aggactaaca gctgtattta tatcattaaa ttcgagcatc ccaagaataa tactagaaaa
     2341 taaaacaact ctaactgaaa taggcgcatt tagtagtttt ttctaccttt tacagattgc
     2401 ccaaattatc attcaatcat tatgccaaac ttactctccg catatatcga gattacacat
     2461 tgaaaaagat aaaaaaaatc tacaaaaaag cataataaat ttattaacat taactttatt
     2521 tatatctatt tcttatattg catcttctct tatattaaaa gatctcattt tttcattact
     2581 atttaataaa gatttttatg catttcacaa gacttatgat tatataacct attattcacc
     2641 actattattt tttatgatag ctcttggaaa tatttcaaca gccttaaaga aaataaagct
     2701 acaaccttat attttctttt tttcaacatt gttgattcta cctttatcca tcatatgcat
     2761 cagtaagtat aatctaaatg gtgcaatatt agcgattagt atttcttctt taataacgat
     2821 atgtttaata attagttata tcaattcaaa gataaaataa ttatgaatca gaaaaaaaaa
     2881 gtattacata taatatcatc actaaatatt ggtggtactc aagttctcct aagagattta
     2941 ttaaaaacca caagtttttc taattatcaa cattactttc ttgtagaaga atcaagtggt
     3001 tttcttgata atgaaataat taaattaggt ggagaaatca ttagagtaaa aaaaataaaa
     3061 aaaacaacat ttatatatta tatatataat cttatacgta taataaaaat aaaaaaaata
     3121 gatatcgttc attctcatgt atcttatttc tctggcatta acatttttat ttctttctta
     3181 tataacgtta aaattagaat atcgcatgca cacagtatga gtaaaaaaaa taataaatac
     3241 acatcactaa tgcgtctttt aattaataaa ttttccacac aaaaaatagc atgttcgcac
     3301 gaaactggga tatcaatctt tggtagcaaa actgatttta ctatcataaa gaatggaata
     3361 tttttaaata agagaaacat aaaacaagaa aacaacatat ccccattaat tataggccat
     3421 attgctagat tagttcccga aaagaaccaa aaatttttaa ttgatttaat tagtaagaaa
     3481 aataatagtg aattacatat tattggcgat ggtccattaa aaaatgaact aatcgaatat
     3541 tcacaaaaaa taggtgtatc agatagggta tattttcatg gcgtatcaag atatccattt
     3601 aaagatatag gtaaatataa atgtaatgtt atgatatatc catcaattag agaaggtttt
     3661 ggaataagta tcattgaatc acaatattat ggatttccaa ctgttatatc aaataatgtt
     3721 cctaaagaag caattgtaat accaagctta gtaaaaacta tatcattaga aaatagcgat
     3781 attgatatag atactttctt aacaaaaaat aaaaaaatat ctcaaactat tatagatgaa
     3841 tattttatta ataaagaatg tgatataaaa tatactgcca ataaaatagg ttcgatctat
     3901 gaaagtaaat aacataattt taaaattata taaaatatca tttacattta ttcaaatgat
     3961 aatagaaaat ataaaaataa atccttttcg atctcatatt catggatata tatctaatat
     4021 aaacggttta tgtattatta aatcaggatt aagaaataaa cgaaacttaa atatcagtat
     4081 taataaaggc agtttaatta taaaagaaaa tgtgttcttt aataataatt gttcgattaa
     4141 cgtcagaaaa tcaataaaaa tagggaaaaa cactatattt ggagaaggag ttaagttata
     4201 tgatcatgac catgattatt caaaaggaat taatcatctt cgctcttcat tcattacagc
     4261 tccaatagtt atcggagaca atgtttggat aggctctaat actattatat taaaaggtgt
     4321 taccataggt aataattgta ttatagcagc aggatctatt attacaaaaa acgtatctga
     4381 taatacaata atcatacaga aaagaacttc tgaatatcat ctcactagtg aaaattaaga
     4441 tcaagataat gagaaactta ataatttcta tactaatcaa tacgttgcta tttggttttt
     4501 tcataatctt aaacttaaac agcttggatt actatataat tcctttatat gtaacaagta
     4561 ttataatagt atattatata attcccggat ttatatataa agattttttc tcgcctactt
     4621 ttattatttc tttaactatc tatcatttat ttatcatagc tcctttatta catattaatt
     4681 ttaattttta ttatatgttt ttctttaaga aatatgatgt atggaaatcc tattataata
     4741 actgggcttc tctaaattta atcggagtaa ttttatttta cttctctttg catttattta
     4801 ataagataaa acctcttata aaaagtaaga aaaaaaacat ttatgatatc agaacagccc
     4861 ctttttattt tattacaatc ttatttttgt tattctcttt ggtaatacaa ttatatgtct
     4921 actataaatt cgggggtgtt ttgggctata tcaaagctaa agagactatt ggtcccgatg
     4981 cttttaaggg attgggaaaa tttgccatta tagccgaatc atttccgatt atatttatgt
     5041 tcttagctgt tttttattat cggaataaaa aattaccatg ggtaaacata tttatattaa
     5101 tgtttatttt tatactactg aaaatattat ttggcggttt atctggcagt agaagcaata
     5161 ctatatgggg attattttgg tgtctagctt taattcattt ttttatcaga aaattaaata
     5221 taaaatttat aactctatgt actgtattta taatatcatt catgcttgtg tatggtgttt
     5281 ataaaaaata caaaactgat tttttctatg caatagaaaa ccatggaatt agttctaata
     5341 attattacaa tacagcaaca gattctattt tattgaaatc tattttaacc gatttctcac
     5401 gtgcagatat tcaatcattt gctatatatt cacaatcaga aggatattat aataaactta
     5461 gatatggaga aacctatata tcttcattat tattatttat accaaattct ttattagatt
     5521 tagaaaggaa aggaagccct ggtgctgaat tattacacca gcaatatgat aaatcaacaa
     5581 gcagagtatt aggattacaa ggtgaattca taattaactt cccatattat ttttatcctt
     5641 tagtctttat tcctatagct ttctttatat ctcgccttca aatgatttat aaaaatagaa
     5701 cagaaaaaca attagattta ttaattcttc cattttttat taatatcggt attattttat
     5761 ttttatctga tagtgataat atagcttact tcattttgaa gaatggcttc attcctatgt
     5821 gtatttatat attgttgaat ttaattactt ataaaaaaaa actttatgac taaaaaaatt
     5881 tgttttctag atagctatca tacaggtctt aatggcgcgc ccaaaagcat gttatctctg
     5941 gctaaaggac tttacgagaa atcatttaat gttactatac ttacaactaa aaatgacctt
     6001 ttattacaaa aagctagtaa tttaaatctt aatactgact tcattaacac tccccaagtt
     6061 ttgcttaaat caagaaaaaa taagagtatt ttttttaaaa tttcttatat aatctctctt
     6121 ttttattttt ggtttttact attaataagt aaaaaccaat actttaaaag atatgatgta
     6181 atatgtatta atgacattag atcattctta ttattcttac cacttttagt tatctacaaa
     6241 aaaaaattaa tatggtatat tagaataaat gaaagaatta aacttattac aaaaacagca
     6301 ttaatattat caaaaaaaat aatattaata tctaaagata gtttttcaat ttttaatgaa
     6361 aatgaaatta aaaaatatag taataagttc tctataatta atacagggtt taatttccca
     6421 tcattaaagg agaaaaatat ttctctacat aaccttcgtg atgacttgca gatagaaaaa
     6481 aatgatattg tatttataag tattggttct atatgtgaaa gaaaaaatca gttatctatt
     6541 ataaatactt ttaaaaaact tcacattgat aacaaaaagt taatactaat aggttcacca
     6601 acttcagata ctgataaact atatgaagct ttattagata aagaaatatt aaaatacggc
     6661 cttaatagaa atattataaa aattccctat agtgataata tatttgatta cttgaaatta
     6721 agccatattt tcctttttgc tagtaaaaag gaaggattac ctagagtttt aatagaagca
     6781 ttaagtactg gatgttattt atgcagttca aatgtagatg gtgttaatga tataattgat
     6841 aatgataaaa taggattaaa aacagatatt tcatcttatg atatttattt tgaagatagc
     6901 tttttaaatt taataaataa tgcattatgc cattattcta aagataagga gtttagaata
     6961 aaatatgtaa gagaaaaatt taattatact aatttcatta acaactttat aaaaactata
     7021 ttaagctaaa taattttaat cttattgtta atataatcat atgaaacatt taaaaaacac
     7081 attcagagat attctaagat tttttttagg taaaaaaatt tacacttata ttagatttat
     7141 acttgtacat aaatatattc caaattttaa acagccatca tctttttctg aaaaaataat
     7201 atttagaaaa ttatatgata atccaaaaaa atattcctct tttgtagata aatatacggt
     7261 tagaaaatat atagaaagta agattgggcc tgattattta attccattac taaaagcaaa
     7321 aaattttata actccttcag actttgattc attacccaat gaattcgtaa ttaaaacaag
     7381 taatgggggg ggaggagaaa atgtactaat agttaataat aaaaacaacc taaacttaga
     7441 tgaaatatgt gacagattta acaattatct taaagtaaaa attggaaata aaatagacga
     7501 atacttttat gatatagaac aacctatgat attgtttgaa aaattattac gagatgattt
     7561 ttatggttat cctcccgatt ttaaaattca tatatttaat ggtataaaga ataaaaaaat
     7621 aattcaaatt gatgaaagta gatttaacca tcataaaaga agtttatata acgaaaaaaa
     7681 agaaaaactt ccttttaata tacaaccaaa atacgaagca gtatctgata atttttcatg
     7741 gccagtaaat tggaatgaaa tgttagatat atctatgaaa ttagctgatg attttaaata
     7801 tgttcgagta gatctttata atgttaatgg taaaatattc ttcggtgaat taacattctg
     7861 ccatggtagt ggttgggaac ctatacatcc taagaaatat gattttttat taggaaaata
     7921 ttggcatgaa tatgaataac aaaaaaatag caataacatc aaacaccagt tggtatttat
     7981 ataactttag aaaaaacact ataatatctt taatagaagc tggatttaaa gtttatactg
     8041 tttcacctat tgatagttat tcaaacaaac taaaagaatt aggtgcagaa catataaata
     8101 tcaaaattga ttcagccagt aaaaatcctt tgattgacct ctatacattg atttcatttt
     8161 ataaaatatt taaacaaatt agatgtaatg ctgtactcaa tttcactcct aaaaataata
     8221 tttatggaac attagcagca aaattaaaca aaataaaagt catcaataat attgcaggtt
     8281 taggtacatt atttgtaaat gatactatca gttgtaaact agcaagaaat ttataccgct
     8341 atagccaaaa atatgctgat aaaatatttt ttcaaaataa cgatgacctt tcattatttt
     8401 tagaaaaaaa atatgtcgat aacgtacaag ttgatagatt gcctggttca ggtgttgatt
     8461 tatctcgctt tacattgtca ttatcagaat ataaacataa atttagattt ctattagtgg
     8521 caagaatgct ttatgaaaaa gggattactt tttatgttga agctgcacgt atcttaaaag
     8581 aaagatatgg cgataaagtt gaattttgtc ttttaggatt tgttggtgta aataatcctt
     8641 cagcaatcac acataaacaa atgaataact gggtagctga gggaattatt aattatttag
     8701 gtacatctga taccgtagaa aatgaattag cccaagcaga ttgtatagtg ctaccttctt
     8761 tttaccgaga aggagtacca aaaacattac tagaagctgg agcaatggga aaacccataa
     8821 tcaccactga taatgttggt tgtcgagaaa cagttactca tggttttaat ggatatatat
     8881 gtcaacctaa gtctgtatca agcttagttg atgccatgga tagatttatt aacttaccct
     8941 atgaaaaaaa actaaaaatg ggtcaaaata gtaggcagaa aatcgaaact gaatttgatg
     9001 aaagaatagt cataaaaaaa tatttagatg cgttaaaaga aattatgtaa ctttaggata
     9061 aaaaatgaaa atcaccatct caggtaccgg ctatgtcggt ctatctaacg ggatactcct
     9121 ttcccagcac aacaaagttg ttgcattaga tatcaataaa gaaaaagtcg aactattaaa
     9181 caaaaaaatc tctcctatct ctgataaaga aattgaagag tttttagcta ctaaagagct
     9241 cgactttaca gcaactttag ataaagaatt cgcttataaa gatgctgaat ttgttatcat
     9301 agccactcct accgattatg atcctaaaac taactacttt aacacctctt ctgtcgagtc
     9361 ggttattcgt gatgttttag aatataatcc aaacaccact atgattataa aatcaaccat
     9421 tccggttggt tttactaaac agatgagaga aaaatatcat accgataata ttatcttctc
     9481 tcctgagttc ttacgtgaag gccgcgccct ttacgataat ctctacccat ctcgtattgt
     9541 gattggtgag caatctgcaa gagcagagaa atttgctgag cttttacaac aaggggcatt
     9601 aaaaaaagat attgatgttc tttttactga ttctacagaa gcagaagcaa tcaaactatt
     9661 tgctaatact tatctcgcac tgcgtgttgc ttattttaat gaactcgata gctacgccca
     9721 agcttacaat ttaaatgcaa gacaaattat tgaaggtgtt tgtttagatc cgcgtattgg
     9781 taatcactat aacaacccct cattcggtta tggcggttac tgcttaccaa aagatactaa
     9841 acaattgtta gccaactacg aatcagtacc taataatatc attggtgcca ttgttgaagc
     9901 taaccgcact cgtaaagact ttattgctga ttccattatc gctaaatcac cgaagattgt
     9961 tggtgtttac cgtttaatta tgaaatcagg ctctgataat ttccgctcat cttcaatcca
    10021 aggcattatg aagcgtatta aagcaaaagg cattgaagtg gtaatttatg agccagaaat
    10081 gaaagaagag actttcttta actcaaaatt aattcataat ttagatgagt ttaaagcaat
    10141 gtctgatgtc attattacca atagaatggc ttcagcacta aacgatgttg aagaaaaagt
    10201 ttatactcga gacctctttg gtaacgatta attaatagaa acggattaat tacgtaatga
    10261 aatatttagt cacaggtgct gctggattta ttggttttca tctaataaaa aaactgatcc
    10321 agcagggtga aaccgttgtt ggtattgata accttaatga ttattatgat gttgctttaa
    10381 aagaagctag acttaatctt ttaaatcaac tggataattt ctctttttct tttattgatt
    10441 tagctgatag agaaaaaatt actcaattat ttgaggctga gaaatttgat agggttattc
    10501 atttagctgc acaagctggt gttagatatt cactaattaa tccgttttct tatgctgata
    10561 gtaatttaac tggttttcta actattttag aaggttgccg acacaataac gttaagcatc
    10621 ttgtttatgc atcatcaagt tcagtctatg gcttaaatga tgaattgcca ttttctcctc
    10681 atgatcaggc taatcatcct gtttctttat atgcagccac taaaaaagct aatgaattaa
    10741 tggcgcatag ctattctcat ttatatggta ttccgactac tggattacgt tttttcacag
    10801 tctatggccc ttggggacgc cccgatatgg cattatttaa atttactaaa gccattatta
    10861 acaatcagcc tatagatatt tataaccatg gtgaaatgaa acgtgatttc acttatgttg
    10921 aagatattgt agaaggtgtt acacgtattg ctgatgtgat ccctacaacc cagcaagatt
    10981 ggaaagtaag tacaggtacc ccggcagata gttctgcacc ctataaggtt tataatattg
    11041 gtaatggatc tccggtaaat ttaatggatt atattagtgc gttagaaata catttaggta
    11101 aaaaagcaga taaaaatatg ttaccaatgc aacccggtga tgtgtacacc acatgggcag
    11161 atactgagga tttatttaaa gcaactggct ataaacctca aacaagtgtt gatgaaggtg
    11221 taaaacagtt tgttgattgg tataaaaact attatcaagt aaaataaata atgctcaata
    11281 tcgtcttatt tgaacccgaa attccaccga atacgggtaa tattatccgc ctgtgtgcaa
    11341 atacaggctt tcatcttcat ttgatccaac cattgggttt tacttgggat gataaacggc
    11401 tacgccgtgc aggacttgat tatcgtgagt ttgctgatat caaacaacac cacgattatt
    11461 atgctttttt agaaagtgaa ggattaagcc ctgataatgc ccaatcgcca acaggggcta
    11521 ggttattcgc attaaccact aaaggcacac ccgcccatag taatgtaagt tatcaagatg
    11581 gggattatct gctatttggc cctgagacac gaggtttacc accttatgtg ttggataata
    11641 tgccaacaca acaaaaaatc cgtgtgccaa tgttagcgga tagccgcagt atgaatcttt
    11701 ctaatacggt tgccgtggtt gtttttgagg catggcgcca attgggctac cctggtgcgt
    11761 tattaagaga ttgatttagt ggttagtttg gttaattaaa gttattttaa ttcatactca
    11821 ttacttgatt ttatctttat ttataaacta aaaaaggtgc taatatttat tcagcacctt
    11881 ttttataaac ctttgttcaa gcattatacc tgcttgatat ccgcaatcac tttcgtgata
    11941 taccgacacc cttcccccac atcctttaat actaatgcat gggcgccaat aatcgcatta
    12001 tcaccaatat taatctcacc accaataatg gatgaattac agccaatata aacatggttt
    12061 cctacttgga tattggcttt tccccctttc tcttttacgc caatagtcac cccttgcata
    12121 atagtgacat attcccccag ttggcagtaa ttagtcagta caatgccaat cgggtgtggt
    12181 aaagaaaggc ctttaccaat acgcgttttt aacgagatat cattacagta tttacggtta
    12241 ttgctagcac cgatccgaag acctaattta tgtaaaaagc gatggggaga gaaataaaaa
    12301 tactgtgcta tccgccacca aaataggtaa cgggaacgct gacagaagaa tacccgatga
    12361 aataatctta accaactaaa gggtttttta tcaccaatat attcagcctg taagcaatct
    12421 tttagttcag caagggtaac cttagcttga gcttgcatag ttaaatacca tcaccacact
    12481 caaagccggg tacaacacca ttaaagtgct gatccatatc aaatgacggt ttttcactat
    12541 caggacgacc gacaatgcgg gcaggcaccc ccgccactgt agtatgagcc ggtacttcat
    12601 gtaatactac cgagccagca ccaattttgg caccacgacc aatctcgata ttaccaagaa
    12661 ttttagctcc cgcaccaatc attacccctt cacgcacttt cgggtgacga tcgccccctg
    12721 ttttacctgt tccccctaag gtcaccgatt gtaaaatgga gacatcattt tcaaccaccg
    12781 ccgtttcacc aatcacaata cctgtcgcat ggtcgagcat aataccatgt ccaatacgtg
    12841 ccgccgggtg aatatccacc ccaaaggtga ctgatatttg gttttgtaga taaatggcta
    12901 gcgcttggcg attttgttgc cataaccaat gagcaatacg gtaggcttgt agtgcatgaa
    12961 agccttttaa atagagcagt ggggtagaat atttatccac cgccggatca cgtaggcgta
    13021 ccgcggagag atcacaagca gccgactcaa tcattgagct atcgtgacga taagcctctt
    13081 caacaatttc gcgtactgca atggctggca tagtctgagt tgccagctta ttggccagca
    13141 tatagcttaa tgcactacct aaattttcgt gtttgagaag tgtcgcatga aagaaactgg
    13201 ctaaaattgg ctcacaatct gccaatgctc gcgcttcgct tttaatatga tcccagactc
    13261 ttgttagttc ttctagcgac atactcactc tcatttttta cacgaaagcc taagtaaata
    13321 cttaggcttt gaattttcaa acaaatggat agttaaaaca gataccctaa gaatattatt
    13381 taaaataaac ttacaatagg ctgctgtcta tctcatcttt ggttgctcgc cctaataaag
    13441 ccaatgcagc ctcttttaca tctttatgtt gatataaaat ctggtagatt tgttcggtga
    13501 ttggcatttc tacacccact tgttcggcta atgcgcgaac ttctttggtg ttacgataac
    13561 cttcaactac ctgcccgatt ttctcttggg ctgtatcaac atcaaaacct tgacctagca
    13621 tcataccaaa gcgacgatta cgtgattggt tgtcagtaca ggttaaaact aaatcaccca
    13681 aaccagccat gcccataaaa gttgccgcat ctgcacctaa ggctttacct aaccgactca
    13741 tttcggccag accacgggta ataagtgcag tacgcgcatt agcgccaaac cccataccat
    13801 cagacatacc cgcaccaata gcaatcacat ttttaacagc gccacccagt tgcacaccga
    13861 taaaatcagg gtttttatag actcgaaagc ttttaccaca atgaaatagc tgttgtaact
    13921 gttctaaaaa gagattgtct gtcgaggcca ctgcaatcgc ggtgggtaaa cccgcggcaa
    13981 gctctttagc aaaggtagga ccagataaca ctgcgagcgg gatttcatta cctaatactt
    14041 cacgggcaac atcttgtaat aagcgaccag tatgtgcttc aagacctttt gtcgcccaaa
    14101 caacacgtgc atcctgacgt aaaaagggtt tgatttgttg cagtacttca ccaaacacat
    14161 ggcttgggat caccacgaga atattacggc tcgcttcaat cgctttttgc aaagaagctt
    14221 ccacatataa actatcagga aaggaaacat cgggcagaaa ggcttgatta cagcgcgctt
    14281 gttctaatgc cgcaacgtga ttaggatcat gtccccacag cacaacatca tggccattac
    14341 gcgctaaggt aatcgctaaa gcggtgccgt atgaaccggc accgataact gtcatagaag
    14401 cgttcattaa gcttcttcaa ctcctgagtt tgctgcatcc tgttgctgtt gttgctgtaa
    14461 atagttcata aacaacgcat caaagttaac aggtgctaag tttaattgtg ggaaagtacc
    14521 acgacctact aagttggtga tacattcacg tgcataagga aatagtacgt tcgggcagta
    14581 tgcacctaag caatgtgcta attgtgtacc ttcgatacct tcaacagtaa agatacctgc
    14641 ttgttgtact tcacacagga aagcggtttc ttcttccatt gttgccgtaa cagtgacacg
    14701 taagataact tcatatacat tttcagctag cgtattagaa gaagtatcta aatctaattt
    14761 aacttctggt tgccactctt tttggaaaac ttgtggggca tttggcgctt cgaaagagat
    14821 atcttttgtg tagatacgtt gaatttgaaa agtcatctct tggttttgct gttctgacat
//
