LOCUS       KY710737               16672 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain CCUG 10702 O antigen gene cluster,
            complete sequence.
ACCESSION   KY710737
VERSION     KY710737.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="CCUG 10702"
                     /serotype="O75"
                     /culture_collection="CCUG:10702"
                     /db_xref="taxon:584"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     gene            1..1251
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16672
                     /note="O antigen gene cluster"
     CDS             1670..2563
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlD"
                     /product="rmlD"
                     /protein_id="AXZ00095.1"
                     /translation="MKIILFGKNGQVGWELQRSLAPLGTVIALDSKSTEYKADFLFPA
                     ELEKTILSIKPDIIVNAAAYTNVDHAETDRINAFKVNFESVEVIAKTAEKIGALLIHY
                     STDYVFDGTNDTPWLETDVPAPLNIYGKSKLAGEQAIINSCKKYLIFRTSWVYASRGN
                     NFAKTMLRLGKKHDSLSVINDQHGAPTSAELLADCTAHAIKSVLDNVSLYGLYHLTAK
                     GTTTWFEYASLLFEYAKEYSLPLSVKNLTPIPAEEYPLPAARPHNSRLNIQKFEKNFK
                     LELPHWKFGIYRMLNEILDNK"
     CDS             2585..3463
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXZ00096.1"
                     /translation="MKKYKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGNGSQWGIHLQYKVQPTPDGLAQAFIIGEDFIGS
                     DNCALILGDNIFYGHDLPKQLEQAIQREQGATVFAYHVNDPERYGVIEFDKNKIAISL
                     EEKPLHPKSNYAVTGLYFYDNSVIDIAKNLKPSARGELEITDLNKVYLKQNNLSVAMM
                     GRGYAWLDTGTHKSLIEASNFIQTLEERQGLKVSCPEEIAYRKGFINKEQVEVLAKPL
                     SKNGYGKYLLKIIQDD"
     CDS             3466..4023
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="rmlC"
                     /protein_id="AXZ00097.1"
                     /translation="MNVTQTEIPDVLILEPKVFGDHRGFFYESFNKKIFEKAINHPVN
                     FVQDNHSKSNKNILRGLHYQTQNTQGKLVRVISGEVFDVAVDIRENSPTFGKYVGVYL
                     SAENKKQLWIPEGFAHGFFVISEEAEFVYKCTDYYNPTAELSIAWNDPDLSINWPIDV
                     DTPIILSMKDKNALSFNQLKSQNIL"
     CDS             4128..5663
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXZ00098.1"
                     /translation="MYNEVNNVKEKVAFNTCIMYIKMALLIGISLYTVRILLNNLGIY
                     NYGIFTLSAGLITVFSFLNSAMSISTQRYLSYFQGSKNIKMQNSVFYSSMILHIIVAT
                     ISILVLLIIMPFLFNGFLNIDSKFISVARILYIYMLISLFFTIVSVPFVATLFSRENI
                     LLDSIILIVQAIMKLIAAHLIIYYPDSEKLSLYGLSLAVISGIVFLFYFIYCCKYYKE
                     CRINKNSNCNGLLKEMAFFAGWNLYTNLCYVLNTQGLNIVLNVFLGTTINAAYGIASQ
                     INGQIRELSLSLLRALNPQIMKSEGMEKRERTIRLSILASKFGFFLVSIVGVPAIFTM
                     SEILKFWLNIVPSYTANICIYLLIASILNQLTVGVTSAIQAIGNIKKFQLVIGSIALL
                     TLPFSYLLLKLGYSFYSVLTLIVVIEVITSFIKIYFFSKISNVSILNYFNNCLVYSLM
                     PLIIATIFLYLLNILIIINYNWVIYFILMFLLYPPLFYLIGFNKNEKSNFKSLLTRIK
                     FKR"
     CDS             5674..6672
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00099.1"
                     /translation="MLSKKKQLPLVSIIVPVYNVEKYLTQCIESIINQDYKNIEIILI
                     DDGSPDQSGQIVDRFALIDPRIKALHINNGGVSAARNLGLSIANGKYIVFVDADDYLS
                     IDYVSYMLNISFETDCDFVISKNCFKVPGNSQQIKNDDINILSPVEAASLLLYPGKVD
                     VGCWNKMYKKQFLLENKITFPENFYMGEGLNFIIRVAQHSNNVGVGKRKVYHYRKDNI
                     TSATTLLSVDKYVNALSAIDNIENHLIYNSKSLKNALKIHKYLTIFATIKAILFMDEI
                     NKYKKQYKFYLHFLRKNIFNLFIASIPPRTKLIISLYCFSPYIGSKITKYIAKLRK"
     CDS             6709..7839
                     /note="orf07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf07"
                     /product="hypothetical protein"
                     /protein_id="AXZ00100.1"
                     /translation="MKNIDISVGILTMHRVINYGSFLQAYATQEIIKSLGFNCEIIDY
                     MFPNKWHYDNGLHNYKNIKSIISDYIYRLGMLKSHRKRKEINIAINKYLNLSYNYKTP
                     DDITKNPPIYDIYITGSDQTWNTKHTRGDTTFLLSFAPKNVKKISFSASIAGNCLDEK
                     YKKDFKKYLSQYNRIAIRDSNGNKVINEINNSYAEVTLDPTLVFNRDQWSQFARNKKQ
                     IFIKSNYIIFYLITHSFDPRPYIYELLYFLQQKTGLKVYSFTKIPKEFNINYEDCSDL
                     SVEHFIQLFENSSYVVTSSFHGTAFATNFGIPLYSVIDDINNQDDRQTSLLSKLEINN
                     CLVSVGTKFEEIDPTYDIEREQKKLNELRSQSINFLKENLIL"
     CDS             7923..8993
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXZ00101.1"
                     /translation="MIVFYIIFFFLATGYLLQLFNIIGNGPYEKILLALIALLLLLIA
                     SFNTTSNDYNAYVQMFQQAAILPFHLYQEIHGEFIFLTISGFIYKTFNSPTNVFLFFS
                     IISIFITFKIIFKDSKLAFLSVLIYLSHAFLNKEMIQIRAGIASAIILFAISMQAKGE
                     NKKALFLIIISSLFHTSALVALLPYTICLVFKNEKQVRIAYILLFLSFVFYLSGGFEL
                     LISILQTLGLLSEKVTTYLGWKEYNYDLGILNPNTIKQLLLLVISIYFFNKSKFYTTY
                     FCYFYIGICWLIGFSSMAIIAARVSSILNVSELILIPTIIINTRKNRIFLGILFLALY
                     TSMFIINIEYKTIIKSLDFGVI"
     CDS             8996..9856
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00102.1"
                     /translation="MNKKFSLILATYNPDPKLLLRLLESLSRQTIKNFELIIVEQTIP
                     PVAKNIIENFHHDFDILFFTSKKGLSRSRNLGSTYANGQFLLFPDDDCWYDDDFFHTI
                     TKYIDTEKNKILTFRAANDEDINIAKFDTTSGFCNKFNIWKRVSSISLCIEKTTFDSL
                     LGFNENLGLGSGTLTSACEDIELPLRAICNKIDVKYTPTIVARHDIPIKRDPKLVIHR
                     AKNHMFSVGYLYKKYDYPIRFVIFSISKNIVALALYSIRLNTTMVKYHYYTAIGKFKG
                     YLANTKLNKN"
     CDS             9861..10907
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00103.1"
                     /translation="MNIAFVVTSLSNKGPIIVIKDIIEHLPIDWNITIYFFDDILEVK
                     FSKKIKLVKVTNFYSKVNLSRYDIVHSHLLRADIFCFLNRKTINKHLSTMHADIIKDL
                     QISHGYLLGGIVGSIWSYILKHSDHVVFLTNLQKKTYSHIKKCSIIYNGRPVIKNNIN
                     MDNYLSQIKSKHCNYILLGACANVVKRKGFDQIMTLMSRNDGKQYLFALVGNGPELDN
                     LKQYAIDLKIQSRCIFIDKTLDVQKYLMLFDIFIMTSHSEGMPLALLEAAACGLPIVC
                     SSLPVIQEIFNSDEVSFYECNNIDSLSEAINYAIDNSLLLSSNVKRKFLEKYTDNIMS
                     KKYQDLYISLLKEN"
     CDS             10993..11766
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXZ00104.1"
                     /translation="MSIYISVISHGHSDLINKLSCLENLSKHYNIIIKSNKFGDDFSK
                     LEENINIHWINNQYNCGFGKNNNIVYSYCVKNLKMVEDDYFIILNPDVIVNYNAINAL
                     IEEMIRDKVLIATINLFKDKKNSIYDCSIRMFPSLKHFIKSFLGLGNSSIVDKSKIIE
                     PCHIDWAAGSFLAIQSCHYLKLGGFDEKYFMYCEDIDICYRSFLMNIPVKYYPAIHAI
                     HLAKHANRKIFSKHFYWHFSSAIRFLLTKKNLTKMKSSI"
     CDS             11885..13051
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXZ00105.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKKLDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSTRAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMSSALNDVEEKVYTRDLFGND"
     CDS             13063..13566
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXZ00106.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNVQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13696..14250)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXZ00107.1"
                     /translation="MHSQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGERCRYITKVIADIRQV"
     CDS             complement(14253..15086)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXZ00108.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15186..16199)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXZ00109.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(16199..16672)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5752 a   2687 c   2647 g   5586 t
ORIGIN
        1 atgctagagc aacatataga agccgaacta gcgcaagatc cggctaacga tctgctatgg
       61 tggcggcgat taatccgcgc cattgataaa tgggcgccac ctggtcaacg tcttattatt
      121 gtcaccagtg aaggtcgtat tattggtgct caacgtaatg aaatacaagt tgtcagaaac
      181 tttatgggac aatctgataa cgcagaccat ccgaaaaaga aaaaatacgg tcgctctgag
      241 atgttaggtc ccttttccat tagagatggt gaagatcact accaacttta cttagtgcgc
      301 ccatcaagta gcccacaatc tgactttatc aatttattat ttgataaacc gcttttatta
      361 ctgatattca ccatgcttat cagtacaccg ctactggttt ggctctcttg gagcttggca
      421 aaaccggccc gaaaactcaa aaatgcggcg gatgatgtgg caaaaggtaa tctgcgccct
      481 caccctgagc tggaaaccgg cccacaagaa tttttagcgg caggcaccag ttttaatcag
      541 atgatcagcg cattagagcg tatggttgaa gcgcaacaac gattgatttc tgatatctcc
      601 catgaattgc gcaccccatt aactcgttta cagcttgcca gtgcattact acgccgtcgt
      661 agcggtgaaa gtaaagagct agagcgtatt gaaacagaaa cacagcggct agatggcatg
      721 atcaatgact tattagtgct ttcccgcaac cagtataaaa atgagttatt acgtgaaacc
      781 gtaaaagcga atgagctttg gaacgatatt ttagataatg cgaaatttga ggcagaacaa
      841 agcaataaaa ccctaacagt aaccgcccca ccaggcccat ggacaattta ttgcaatcct
      901 tattcattag cgagtgcctt tgaaaatatc gttcgcaatg ccttgcgtta ttcacattct
      961 cgtattgaag tagcctttac cgaacaaaat cagggtatta ctattattgt tgatgatgat
     1021 ggcccagggg ttagcccaga agatcgcgag catattttcc gaccttttta tcgtacggac
     1081 gaagcacgtg atagagaatc cggcggtaca gggctgggac tggcaattgt tgaaaccgcg
     1141 attagtcaac atcgaggcca tgtaaaagcc gatgatagcc cattaggggg attaagagtg
     1201 gagatttggt tgccgagagg gagtgtggta aataaatcac aacacgctta attcagtaat
     1261 aaaattacta tattaaatta ttatgccatc tattgaagaa taggtggcat attcttttca
     1321 ttcccatctt tatagatatt tcagaatgat cttgctaatt cagtcgatat tacgatattt
     1381 atatatacct aatcaatgtt agaataccca tcaattactt gacccgcggg tttgaagagt
     1441 ctaatctcag agcggtttta cgtactgtta aagtatactt atataataaa actcttatat
     1501 tacagataga taagggtttt aaaagaaaat tttggcgctc tgggggccgt aaacccagtg
     1561 cggtagcttt gtacgtatga taagtatgat aattatcata atatagatat aaaaccttat
     1621 actctattcg aatgtatatt atattttatg agttgctaaa aactagataa tgaaaataat
     1681 tttatttgga aaaaacgggc aagtcggttg ggaattacag cgttctctag caccattagg
     1741 aactgttatt gctttagatt ctaaatcaac agaatataaa gctgattttt tatttcctgc
     1801 agaactagag aaaactattt tatctattaa accagatatt atcgtcaatg cagcagccta
     1861 tacaaatgta gatcatgctg aaactgatcg tattaacgca tttaaagtaa attttgaaag
     1921 tgttgaagta attgcaaaaa cagctgaaaa aattggggct ttattaatac attattcaac
     1981 agactatgta tttgatggta ctaatgatac tccttggtta gaaacagatg ttccagcacc
     2041 tttaaatatt tacggaaaaa gcaaactagc gggcgaacaa gctattatta atagctgtaa
     2101 aaaatatcta atttttagaa ctagctgggt atatgcaagt agaggtaata actttgcaaa
     2161 gaccatgcta agattaggaa agaaacatga ttcactatca gtgattaatg accagcatgg
     2221 ggcccccaca agtgctgaat tattagcaga ttgtactgct catgcaataa aaagtgtatt
     2281 agataatgta tcgctttatg ggttatacca tttaacagca aaaggaacaa caacatggtt
     2341 tgaatatgca tcattactat ttgaatatgc aaaagaatat tctttaccat tatcagttaa
     2401 aaacttaact ccaatccctg cagaagaata tcctttaccc gcagctagac cacataattc
     2461 acgtttaaat atacaaaagt tcgaaaaaaa ctttaaacta gaattacctc actggaaatt
     2521 tggcatatac agaatgttaa atgaaatttt agataacaaa taattatcaa tatatagttt
     2581 aaaaatgaaa aaatataaag gtattattct tgctggtggt tcaggaactc ggctttatcc
     2641 ggtaacaatg gctgtaagta aacaattgtt gccaatttat gataaaccaa tgatttatta
     2701 tcctcttagt accctgatgc tcgcaggaat aagagatata ttaattatta gcacccctca
     2761 agatacacca agatttcaac aactattagg aaatggtagc caatggggta ttcatcttca
     2821 atataaagtc caacctaccc ctgacggctt agcacaagca tttattattg gtgaagattt
     2881 tattggctca gataattgtg ctctaatttt aggtgataat attttttatg gccatgattt
     2941 acctaaacaa ttagaacaag cgatacaaag agaacaaggt gctacagtat tcgcctatca
     3001 tgttaatgat cctgaaagat atggtgttat tgaatttgat aaaaataaga ttgctatttc
     3061 attagaagaa aaaccattac accctaaaag taattatgct gttactggtt tatacttcta
     3121 tgataacagt gttattgata ttgcaaaaaa cttaaaacct tcagctcgtg gtgagttaga
     3181 aatcactgat ttaaataaag tatatttaaa acaaaataat ctctcagttg ctatgatggg
     3241 acgtggatat gcttggctag atacaggaac acataaaagc cttattgaag cgagtaattt
     3301 cattcaaact ttagaagaaa gacaaggatt aaaagtttct tgtccagaag aaattgctta
     3361 tagaaaaggt tttattaata aagagcaagt agaagtactt gctaagcctc tatctaaaaa
     3421 tggttatggt aaatatttat taaaaataat tcaggacgat taattatgaa tgttactcaa
     3481 acagaaatac ctgatgtatt aattttggaa cctaaagtat ttggtgatca cagaggtttt
     3541 ttttatgaaa gttttaataa aaaaatattt gaaaaagcaa ttaaccatcc tgtaaacttt
     3601 gttcaagata atcattccaa atcaaataaa aatatattaa gaggtctaca ttatcaaact
     3661 caaaatactc aaggtaaatt agtacgggtt atatcaggtg aagtttttga tgttgctgta
     3721 gacattagag aaaactctcc aacatttggt aaatatgtag gagtatatct ttccgcagag
     3781 aataaaaagc agctttggat acctgaaggc tttgcacatg gcttttttgt tataagtgaa
     3841 gaagccgaat ttgtttataa atgtacagat tattataatc ctacagcaga attatctata
     3901 gcctggaatg atcctgattt atctattaac tggccaattg atgtagatac cccaattata
     3961 ttatcaatga aagataaaaa tgcactatct tttaaccaat taaagagtca aaatatacta
     4021 taatttattc tattctcttt catacttata atctctaaat ttattattaa attatattta
     4081 gtgattatcg aatagaacat tttagcaaga attaagaaat ttaaacaatg tataatgaag
     4141 ttaataatgt aaaagaaaaa gttgcattta atacttgtat aatgtatata aaaatggcct
     4201 tgttaattgg tatttcttta tatacagtac gaatactttt aaataattta ggtatatata
     4261 actatggaat atttacacta agtgctggcc taataactgt attttcattt ttaaattcag
     4321 caatgtctat atcaacccaa cgctatttat cttatttcca aggatcaaaa aatattaaga
     4381 tgcaaaatag tgttttttat tcgagtatga tcttacatat tattgttgca actatatcaa
     4441 tattagtact actcatcata atgccatttt tatttaatgg ttttttaaat atagatagta
     4501 aatttatatc cgtagctcgt atactatata tttatatgct catctcttta ttttttacaa
     4561 tagtttccgt tccatttgta gcaactttat tttctcgaga aaacatttta cttgactcta
     4621 taattcttat cgtacaagct ataatgaaac ttatagcagc tcatttaatt atttattatc
     4681 ctgactcaga aaaactttct ctttatggtc tctctttagc agtaattagt ggtatagtat
     4741 ttttatttta ttttatatat tgttgtaaat attataaaga atgcaggata aataaaaata
     4801 gcaattgcaa tggtttgcta aaagaaatgg cttttttcgc aggatggaat ttatacacta
     4861 atttatgcta cgttttaaat actcaaggat taaatattgt tttaaatgtt tttttaggta
     4921 caacaattaa tgctgcatat ggaattgcat ctcaaataaa tggtcaaata agagagttat
     4981 ctttatcttt attaagagct ttaaatcctc aaataatgaa aagtgagggt atggagaaga
     5041 gagaaagaac gattagacta tcaatactcg ccagtaaatt tggttttttc cttgtctcca
     5101 tagtaggtgt acctgctata ttcacgatgt cagaaatatt aaaattctgg cttaacatcg
     5161 tccctagtta tactgctaat atctgtatat atcttctaat tgctagcata ttaaatcaat
     5221 taactgtagg tgtaacatct gcaatacaag ctattggaaa tataaaaaaa ttccaattag
     5281 ttatcggttc tattgcactc ttaacactac ctttcagcta tttattatta aaattaggtt
     5341 actcatttta tagtgtttta acattaatcg tagtgataga agtaattaca agctttataa
     5401 aaatatattt cttttctaaa atttctaatg ttagtattct aaattatttt aataattgtc
     5461 ttgtctattc actaatgcca ttaataatag ctactatttt cttatactta ttaaatattt
     5521 taattattat taattataat tgggttatat attttatatt aatgttttta ctttatccgc
     5581 cattatttta tctaatcggc tttaataaaa atgagaaatc taattttaaa tccttattga
     5641 ctagaatcaa atttaaacgt taagaaaaat atcatgcttt caaaaaaaaa acagcttcct
     5701 cttgtttcta taattgtccc agtatataac gttgaaaagt atctcacaca gtgtatagaa
     5761 tcaattataa accaagacta taagaatatt gaaataattc taatagatga tggttctccg
     5821 gatcaatcgg gacaaatagt ggatagattt gcactgattg atcctagaat aaaggcatta
     5881 catattaata atggtggtgt tagcgctgca agaaatcttg gtttatctat tgcaaacggc
     5941 aaatatatcg tttttgttga tgccgatgat tatctaagta ttgactatgt ttcttatatg
     6001 ttaaatatta gttttgaaac agattgtgac tttgtaatat caaaaaattg ctttaaagtt
     6061 ccaggtaatt ctcaacaaat aaaaaatgat gatattaata ttttatctcc tgttgaagct
     6121 gcatctttat tactttatcc aggtaaggta gatgttggtt gttggaataa aatgtataaa
     6181 aaacaatttt tattagaaaa taaaattact tttcctgaaa atttttatat gggagaaggc
     6241 ctaaatttta ttattagggt agcacaacat tcaaataatg ttggtgttgg taaaagaaag
     6301 gtatatcatt atcgtaaaga taatataaca agtgcaacaa ctttattaag tgttgataaa
     6361 tatgtcaatg cattaagtgc tattgataat attgaaaatc atttaattta taactctaaa
     6421 tcattaaaaa atgctcttaa aattcataaa tatttaacta tttttgctac tattaaagct
     6481 attttattta tggatgaaat taataaatac aaaaaacaat ataaatttta tctgcatttt
     6541 ttaagaaaaa atatatttaa tttatttata gcttcaatac ctcctagaac aaaattaata
     6601 atatcactat actgttttag cccatacata ggttcaaaga taactaaata tatagcaaaa
     6661 cttagaaagt aatattctaa ttattttaca cataacttta ggaagtatat gaaaaatata
     6721 gatataagcg ttggtatatt gaccatgcat cgcgtaataa attatggttc ttttttacag
     6781 gcatatgcta cacaagaaat tattaaatct ttaggtttta actgtgaaat tatagactat
     6841 atgtttccca ataagtggca ttacgataat ggattacata attataaaaa tattaagtca
     6901 attatttctg attatatata tagattagga atgctcaaaa gtcatagaaa aagaaaagaa
     6961 ataaatattg caataaataa atatcttaat ctttcttata attataagac accagatgac
     7021 attaccaaaa accctccaat atatgatata tacataacag gaagtgacca aacttggaat
     7081 actaaacata ctagaggtga tacaactttt ttattatcat ttgcaccaaa aaatgttaaa
     7141 aaaatatctt tttccgcgag catagcagga aactgtttag atgaaaaata taaaaaagac
     7201 tttaaaaaat atttatcaca atataataga attgctattc gtgattcaaa tggaaataaa
     7261 gtaattaatg aaataaacaa tagttatgct gaagtaactt tagatccaac attagttttc
     7321 aatcgtgacc aatggagtca gtttgccaga aataaaaaac aaatttttat aaagtcaaat
     7381 tatataatat tttacttaat tacccattct tttgatccta ggccttatat atatgagtta
     7441 ctatactttt tacaacaaaa aacaggatta aaagtatact cttttacaaa gatccctaaa
     7501 gaattcaaca tcaattatga agattgttca gatttaagtg tagaacattt tattcaatta
     7561 tttgaaaact cttcatatgt agtaacttcg tcattccatg gaacagcttt tgctactaat
     7621 tttggaattc cactctattc agttattgat gacataaaca atcaagatga taggcaaaca
     7681 tctttacttt caaaattaga aattaataat tgcttagttt ctgtaggaac taaattcgaa
     7741 gaaatagatc ctacatatga tatagaaaga gaacaaaaaa aattaaatga gctaagaagt
     7801 cagtcaataa attttttgaa agaaaactta atattataat atgtttttaa ctttaatcat
     7861 atattcaata ttaatttata tattgttgat attatactcc ccaagaaagt ggttatgtta
     7921 taatgattgt tttttatatt attttctttt tcttagcaac tggataccta ttacagcttt
     7981 tcaatataat aggaaatgga ccatacgaga aaatcctttt agcactcatt gcattactat
     8041 tattattaat agcaagtttt aataccacta gtaatgatta taatgcttat gtacaaatgt
     8101 ttcagcaagc ggctatatta ccatttcatt tatatcaaga aatacatggt gaatttattt
     8161 ttttgactat atctggattt atttataaaa catttaattc acccacaaat gtttttttat
     8221 ttttttcaat tatcagcata tttataacat ttaaaattat atttaaagat agtaaattag
     8281 cctttctctc tgtattaata tatttgtcac atgctttttt aaataaagaa atgattcaaa
     8341 taagagcagg aattgctagc gcaataatat tgttcgctat ttctatgcaa gcaaaaggag
     8401 aaaataaaaa agcccttttt ttaattatta tatctagtct atttcatact tcagcacttg
     8461 tagcattact accatatacg atatgcttag tattcaaaaa tgaaaaacaa gttcgtatag
     8521 cttatatttt attattttta tcttttgttt tttatttatc aggagggttt gagttattaa
     8581 tatctatact tcaaacatta ggccttttat cagaaaaagt aacaacctat ttaggttgga
     8641 aagaatataa ttacgactta gggattttaa acccaaatac tataaagcag ttattgttat
     8701 tagttatttc aatttacttt tttaataaaa gtaaatttta tactacttat ttttgttatt
     8761 tttatattgg tatttgctgg cttattggtt tttcttctat ggctattatt gcagcaagag
     8821 tctcttctat acttaatgta tctgagttaa tattaattcc tactattatt attaatacaa
     8881 ggaaaaatag aatattttta gggattctat ttttagcttt atacacctca atgtttatta
     8941 ttaacattga atataaaaca attattaaaa gtcttgattt cggagttatt tagtgatgaa
     9001 taaaaaattt agccttattt tggcaacata taatcctgat cctaaattgc tacttcgttt
     9061 gctcgaaagt ttatcaagac aaactattaa aaattttgaa ttgataattg tagaacaaac
     9121 tattcctcca gtagctaaaa atataattga aaactttcac cacgattttg atatattatt
     9181 ttttacaagt aaaaaaggcc tctctcgttc tagaaattta ggaagcactt atgctaatgg
     9241 acaattctta ttattccccg atgatgattg ttggtatgat gatgactttt ttcatacaat
     9301 aactaaatat attgatacag agaaaaataa aatattaaca tttagagctg ccaacgatga
     9361 agatataaat attgcaaaat ttgatacaac ttctggtttc tgtaataaat ttaatatttg
     9421 gaagcgagtt tcatcaataa gtttatgtat agaaaaaact acatttgatt cacttttagg
     9481 ttttaatgaa aatttaggtt taggttctgg tacattgact agtgcatgtg aagacataga
     9541 actaccactc agagcaatat gtaataaaat tgatgtcaaa tatactccaa ctattgttgc
     9601 taggcatgat atcccaataa aaagagatcc aaaattagtt atacatcgag caaaaaacca
     9661 tatgtttagt gtcggttact tgtataaaaa atatgactat ccaattagat tcgttatctt
     9721 ttcgatctca aaaaacatag tcgcattagc cttatactct ataaggctaa atactactat
     9781 ggtaaaatac cattattata ctgctattgg taagttcaaa ggctatcttg caaatactaa
     9841 attaaataaa aattaatatt atgaatattg ctttcgttgt tacatctctt tcaaataagg
     9901 gtccaattat agttattaaa gatataatag aacatttacc tatagattgg aatatcacaa
     9961 tttatttctt tgatgatatc ttagaagtaa aattctcaaa aaaaataaaa ctagtaaagg
    10021 tcactaactt ctattcaaaa gttaatttat ccagatatga tattgtgcat tcgcatttgt
    10081 tgcgagcgga tatattctgt tttcttaatc gaaaaactat aaacaaacat ttatcgacta
    10141 tgcatgcaga tataataaaa gatcttcaaa tttctcatgg atatttatta gggggaatag
    10201 taggttcgat ttggagttat atattaaaac attcagatca tgttgttttt ttgaccaact
    10261 tacaaaaaaa aacatatagt catattaaaa aatgttcaat tatatataat ggtagacctg
    10321 ttattaaaaa taatatcaat atggataatt acctctctca aataaaatct aaacattgta
    10381 attatatatt acttggagca tgtgcaaatg tagtcaaaag aaagggtttt gaccaaataa
    10441 tgactctaat gagtagaaat gatggtaaac aatacttatt tgcattagtt ggtaatggtc
    10501 ctgaattaga taatttaaag caatatgcta ttgatttaaa gatacaatca cgatgtattt
    10561 ttattgataa gacattagat gtacagaaat atttaatgtt atttgatatt tttattatga
    10621 cttctcattc agaaggtatg cctttagcat tattagaagc agctgcatgt ggactaccga
    10681 ttgtatgctc ttctttacct gtaatacaag aaatatttaa ttccgatgaa gtttcttttt
    10741 atgaatgcaa caacatagac tctctatctg aggctataaa ttatgctata gataactcat
    10801 tattattgtc aagcaatgtt aaaagaaaat ttttagaaaa atatacagac aacataatgt
    10861 ctaaaaaata tcaagattta tatatttctc tacttaaaga aaattaatat tagaataaag
    10921 ttatttgtaa gtatattata tattaatttt ttaatcttgt ctgcaaaaac tacttaggtt
    10981 aggtatttaa aaatgtctat ttatatttca gtcatatctc atggacattc tgatttaatt
    11041 aataaactat catgcttaga aaatttaagc aaacattaca acataataat aaaaagtaat
    11101 aaattcggtg atgacttttc aaaactagag gaaaatataa atattcactg gattaataat
    11161 caatataatt gtggatttgg aaagaacaat aatattgttt actcatattg tgtcaaaaat
    11221 ttaaagatgg tagaagatga ttattttatt atattaaatc ctgatgtaat agttaattac
    11281 aatgctataa atgcattaat cgaagagatg atcagggata aagttctaat agctacaatt
    11341 aatttgttta aagataaaaa aaatagtatt tatgattgtt ctataagaat gttcccatct
    11401 ttaaaacatt ttattaaatc ttttttaggg ctaggtaatt cttctattgt tgataaaagt
    11461 aaaataattg aaccttgcca tattgattgg gcggcgggtt cttttcttgc aatccaatct
    11521 tgtcattatc ttaaactagg tggatttgat gagaaatatt ttatgtactg tgaagatatt
    11581 gatatttgtt atagaagctt tttaatgaac atacctgtta aatattaccc tgctattcat
    11641 gcaatacact tagctaaaca tgccaataga aaaatttttt caaaacattt ctattggcat
    11701 ttttctagtg ctattcgatt tttattgaca aaaaaaaatc taacaaaaat gaaatcaagt
    11761 atttaataat acttaatgac agaatgaaaa aatattaact taatccggct ctaataaaat
    11821 ttttatagat agatactcta aataaatttt aatgataatt tatagtttta gataagagaa
    11881 aaacatgaaa atcaccatct caggaaccgg ctatgtcggt ctatctaacg ggatactcct
    11941 ttcccagcac aacaaagttg ttgcattaga tatcaataaa gaaaaagtcg aactattaaa
    12001 ccaaaaaatc tctcctatct ctgataaaga aattgaagaa tttttagcca ctaaaaagct
    12061 cgattttacg gcaactttag ataaagaatt cgcttataaa gatgctgaat tcgttatcat
    12121 tgccactcct accgattatg atcctaaaac taactacttt aacacctctt ctgtagagtc
    12181 ggttatacgt gatgttttag aatataatcc aaacaccact atgattataa aatcaaccat
    12241 tccggttggt tttactaaac agatgagaga gaaatatcat accaataata ttatcttctc
    12301 tcctgagttc ttacgtgaag gccgcgccct ttacgataat ctctatccat ctcgtattgt
    12361 gattggtgag caatctacaa gagcagagaa atttgctgag cttttacaac aaggggcatt
    12421 aaaaaaagat attgatgttc tttttactga ctctacagaa gcagaagcaa tcaaactatt
    12481 tgccaatact tatctcgcac tgcgtgttgc ttattttaat gaactcgata gctacgccca
    12541 agcttacggc ttaaacgcaa gacaaattat tgaaggtgtc tgtttagatc cgcgtattgg
    12601 taatcactat aacaacccat catttggtta tggcggttac tgcttaccaa aagatacgaa
    12661 acaactgtta gcaaattacg aatccgtgcc taataatatt attggtgcca ttgtggaagc
    12721 taaccgcact cgtaaagact ttattgctga ttctattatc gctaaatcgc cgaaaattgt
    12781 tggtgtttat cgtttaatta tgaaatcagg ctctgataat ttccgctcat cttcaatcca
    12841 aggtattatg aagcgtatta aagcgaaagg gattgaagtg gtgatttatg agccagaaat
    12901 gaaagaagag actttcttta actcaaaact cattcgtgat ttagatgcgt ttaaagccat
    12961 gtctgatgtc attattacta atagaatgtc ttctgcacta aacgatgttg aagaaaaagt
    13021 ttatactcgt gatctctttg gtaacgatta aaactaattt atatgctcaa tatcgtctta
    13081 tttgaacccg aaattccacc gaatacgggt aatattattc gcctgtgtgc caacacaggc
    13141 tttcatcttc atttgatcca accattgggt tttacttggg atgataaacg gctacgtcgt
    13201 gcagggcttg attatcgtga gtttgctgat atcaaacagc accacgatta ttatgctttt
    13261 ttagaaagtg aaggattaag ccctgataat gtacaatcac cgacaggtgc gcggttgttt
    13321 gcattaacca ctaaaggtac tcccgcccat agtaatgtaa gttatcaaga tggggattat
    13381 ctgctatttg gccctgagac acgaggttta ccaccttatg tgttggataa tatgccaaca
    13441 caacaaaaaa tccgtgtgcc aatgttagcg gatagtcgta gtatgaatct ttctaatacg
    13501 gttgctgtgg ttgtttttga ggcatggcgc caattaggct atccgggtgc gttattaaga
    13561 gattaattta gtggttagtt tgaataatga aagttacttt cattcatact cattacttga
    13621 ttttatcttt atctataagc taaaaaaggt gctaataatt atttagcacc tttctcataa
    13681 gcctttgttc tgcatttata cctgcctgat atccgcaatc actttcgtga tataccgaca
    13741 cctttccccc acatccttta atactaatgc atgggcgcca ataatcgcat tatcaccaat
    13801 attaatctca ccaccaataa tggatgaatt acagccaata taaacatgat ctcctacttg
    13861 gatattggct tttccccctt tctcttttac gccaatagtc accccttgca taatagtgac
    13921 atattccccc agttggcagt aattagtcag tacaatgcca atcgggtgtg gtaaagaaag
    13981 gcctttacca atacgggttt ttaacgagat atcattacag tacttacgat tattgctagc
    14041 accgatccga atacctaatt tatgtaaaaa gcgatgggga gagaaataaa aatactgtgc
    14101 tacccgccac caaaataggt aacgggaacg ctggcagaaa aatactcgat gaaatagtct
    14161 taaccaacta aagggttttt tatcaccaat atattcagcc tgtaagcact cttttagttc
    14221 agcaagggta accttagctt gggaatgcat agttaaatac catcaccaca ttcaaagccg
    14281 ggtacaacac cattaaagtg ttgatccata tcgaatgacg gtttttcact atcaggacga
    14341 ccgacaatgc gggcaggcac cccggccacc gtagtatgag ccggtacttc atgtaatact
    14401 accgagccag caccaatttt agcaccacga ccaatctcga tattaccaag aattttagct
    14461 cctgcgccaa tcattacccc ttcacgcact ttcgggtgac gatcgcctcc tgttttacct
    14521 gttcccccta aggtcacaga ttgtaaaatg gagacatcat tttcaaccac cgccgtttca
    14581 ccaatcacaa tacctgtcgc atggtcgagc ataataccgt gtccaatacg tgccgctggg
    14641 tggatatcca ccccaaaggt gactgatatt tggttttgta gataaatagc tagcgcttgg
    14701 cgattttgtt gccatagcca atgggcaata cggtaggctt gtagtgcatg aaaccctttt
    14761 aaatagagca gtggggtaga atatttatcc accgcgggat cacgtaggcg taccgcggag
    14821 agatcacaag cagccgactc aatcattgag ctatcgtgac tataagcctc ttcaacgatt
    14881 tcgcgtactg caatggctgg catagtctga gttgccagct tattggccag catatagctt
    14941 aatgcactac ctaaattttc gtgtttgaga agtgtcgcat gaaagaaact ggctaaaatt
    15001 ggctcacaat ctgccaatgc acgcgcttcg cttttaatat gatcccagac tcttgttagt
    15061 tcttctagcg acatactcac tctcattttt tacacgaaag cctaagtaaa tacttaggct
    15121 ttgaattttc aaacaaatgg atagttaaaa cagataccct aagaatatta tttaaaataa
    15181 actgactaca ggctgctgtc tatctcatct ttggttgctc gccctaataa agccaatgca
    15241 gcctctttta catctttatg ttgatataaa atttggtaga tctgttcggt gattggcatt
    15301 tctacaccca cttgttcggc taatgcgcga acttctttgg tgttacgata accttcgact
    15361 acctggccaa ttttctcttg ggctgtatca acatctaaac cttgacctag catcatacca
    15421 aagcgacgat tgcgtgattg gttgtcagta caggttaaaa ctaaatcacc caaaccagcc
    15481 atgcccataa aagttgccgc atctgcacct aaggctttac ctaagcggct catttcggcc
    15541 agaccacggg taatcagtgc ggtacgcgca ttagcgccaa atcccatacc atcagacata
    15601 cccgcaccaa tagcaatcac gtttttaaca gcgcccccca gttgcacacc gataaaatca
    15661 gggtttttat agactcggaa gcttttacca caatgaaata gctgttgtaa ctgttctaaa
    15721 aagagattat ccgtcgaggc cactgcaatc gcggtgggta aacccgctgc aagctcttta
    15781 gcaaaagtag ggccagataa tactgcgagc gggatttcat tacctaatac ttcacgggca
    15841 acatcttgta ataagcgacc agtatgtgct tcaagacctt ttgtcgccca aacaacacgt
    15901 gcatcctgac gtaaaaaggg tttgatttgt tgcagtactt caccaaacac atggcttggg
    15961 atcaccacaa gaatattacg gctcgcttca atcgcttttt gcaaagaagc ttccatatat
    16021 aaactatcag gaaaggaaac atcgggcaga aaggcttgat tacagcgcgc ttgttctaat
    16081 gccgcaacgt gcttgggatc atgcccccac agcacaacat cgtgcccatt acgcgctaag
    16141 gtaatcgcta aagcggtgcc gtatgaaccg gcaccgataa ctgtcataga agcgttcatt
    16201 aagcttcttc aactcctgag tttgctgcat cctgttgctg ttgttgctgt aagtagttca
    16261 taaacaacgc atcaaagtta acaggtgcta agtttaattg tgggaaagta ccacgaccta
    16321 ctaagttggt gatacattca cgtgcataag ggaataatac gttcgggcag tatgcaccta
    16381 agcaatgtgc taattgtgta ccttcgatac cttcaacagt aaagatacct gcttgttgta
    16441 cttcacacag gaaagcggtt tcttcttcca ttgttgccgt aacagtgaca cgtaagataa
    16501 cttcatatac attttcagct agcgtattag aagaagtatc taaatctaat ttaacttctg
    16561 gttgccactc tttttggaaa acttgtgggg catttggcgc ttcgaaagag atatcttttg
    16621 tgtagatacg ttgaatttga aaagtcatct cttggttttg ctgttctgac at
//
