LOCUS       KY710689               16016 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 17/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710689
VERSION     KY710689.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 17/57"
                     /serotype="O8"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..1250
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16016
                     /note="O antigen gene cluster"
     CDS             1820..3016
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99381.1"
                     /translation="MLKSQLNYFNPTIIFVLGWMFFCLLPLIYFGEPPLLIIMLNIVI
                     VFSFIIGCVLSYSSKNWISYVLYDNKYSRIKSLLSILCFLYIIIKLYLISTTEINSGN
                     YSEKYAVESQFYLYLMIFDKLFNVIFLFFLSLFAIEGKIKFYFVFSISLLSTFFEPTR
                     LLIILTLVYWILLGVSIGYIKIRLIQVILIALASPFIFSFLLIKRVYSFEGSSYDFIF
                     HLFKLIDYDLLVSGMINGLETFESYKALINVINDNFIHPLSGFVRILFMPIPRTFWID
                     KPESIARLIAENYYPEAYQKGGGQLAGPIGDGFINAGFLGVFIIWLALGFISVSFYKY
                     FKRGVMKGGNIEKAYYCTFYYIYLIYFINCLRGFASDLFWVFAVNMIMLYIIKVIFFK
                     RIQYKE"
     CDS             3070..4173
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99382.1"
                     /translation="MNLIFCGNLHNGGGVQVAISFIQELVTNKITDLSNTDIIISSKI
                     EEELEKIKINLNEFKNAYTMNFYGLRKNKDFKVINNKYNICFIIFGPIYYSLNSEKYI
                     VGFAQPWIAYKDNDVYLKMSLISKIKNKIFFTVKDLIFRKYKHLVVEHEHVKTALLEK
                     NYKSKIEVVSNTYSSIFDDKNSWDKIDLPKGLDNSKFTFGYIGRSYLHKNLKILKKVN
                     QILLSKYNIDVNFLFTLSNEEMKILEFDNIPNFYNTGCITLNQCPNFYNNIDALIFPT
                     MLECFSATPIEAMKMNTPVFCSNYPFLTEICKNAALYFDATSSEDIAKLIAGSINNKN
                     LLEDKIQNGLKVVNTLPSSYDRAMKYINLMNNI"
     CDS             4181..5380
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99383.1"
                     /translation="MKKNSFLKSGSYLLISVMFAKAIAFILNILCARFLDKESFGQLS
                     FIRNTVAMFEGLVSGSNGNLIISSLGNKNYYSSIILSFSFYISISILVFIILLKTSYG
                     GFILIDNYSFLLMIVMFLSSSFLGIINSIYIAHQENKIMVISSFFSGIVSFIICTLLI
                     YYGSYHGALLAYAVYFSIDFFFKIYFIFKKVIKLKFTRFVFDTSILKRSFQLLIFTSL
                     SLCFFWYIRNKLVIEKNGLSELAEFELVYQFVTIALLAIGAFTSTMLSKISHENGILI
                     KTIIISLVFSLTSYLVACIFGETLFKAINPLYNNIGKLIPYTVFIIFPYTASSIVNKL
                     LIIHKLEYISVHSSFFAVLVCSCFFLLDKPLSAMTLSILYCVYYFLVTSISLSYFFLL
                     KKVRPNY"
     CDS             5469..6557
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99384.1"
                     /translation="MMKKKVLILQPVIPNYRLDFFDKLYNDESIDLKIKASNEDMIGV
                     KTCSEAKEREYIELIGNFMSFFNLFFWQRGLRLKSILWADVVVISGNPRLLNHVLFLF
                     FCKITKRKVVWWGQGWTAGSHGLNAKIRRQLMRFADALVLYTENEAMEFSNHKLAIGL
                     NNGLNTKKIRKELSTVISQRDENSDVIHLCFIGRLTQKSKLDILLSSLRDVDRKVKLS
                     IIGDGILRSQLEKFAHDNLKLKNNIDIQFYGAIYNEHDIGEITQVSDAFIYPGAVGLS
                     LIHAYCHGLPALVHGDSNNHMPEYAAFKLGINGLNIPQKSKDLSKFINSIDLLQLRNM
                     RDAAINTVINTFNTDDMAKRFVSIIKKD"
     CDS             6563..7582
                     /note="UDP-N-acetyl-glucosamine 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlA"
                     /product="fnlA"
                     /protein_id="AXY99385.1"
                     /translation="MFKDKTLLITGGTGSFGNAVLNRFLETDIKEIRIFSRDEKKQDD
                     MRKKYNNHKLKFYIGDVRDYSSILNATRGVDFIFHAAALKQVPSCEFHPIQAVKTNIL
                     GTENVLEAAIANSVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNLDSNKTVIC
                     GTRYGNVMASRGSVIPLFVDLMSEGKAITITDPNMTRFMMTLEDAVDLVLYAFQNGNN
                     GDIFVQKAPAATIETLAIALKELLNTPEHPINIIGTRHGEKLYEALLSREEMLAAIDM
                     GDYYRVPPDLRDLNYDKYVEQGDSRISEIEDYNSHNTTRLDVNEMKKLLLKLPLIKNL
                     HNNKI"
     CDS             7595..8695
                     /note="UDP-2-acetamido-2,6-dideoxy"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlB"
                     /product="fnlB"
                     /protein_id="AXY99386.1"
                     /translation="MKILVTGADGFIGKNLCVRLAENNFELIKIDRNSSKHDLELGLM
                     NANFIFHLAGINRPKNESEFHEGNASLTEEITHFLIENKKKTPIMLSSSIQANNNNKY
                     GESKKLAETHIKAYGLKTHANYYIYRLPNVFGKWCKPNYNSFIATFCHNIANDIDIII
                     NSPTAKVSLIYIDDLCNELISLLNGNNRIGFQNISPIYTTTVGEVAELLYAFKNSRNT
                     LVTENVGVGFTRALYSTWLSYLSPQQFTYSVPHYEDQRGVFCEMLKTQNSGQFSFFTA
                     HPGITRGGHYHHTKTEKFLIIKGKALFKFENIITGEKYELTVSDHDYKIVETVPGWSH
                     DITNIADSELIVMLWANEIFNKEFPDTIARPL"
     CDS             8695..9825
                     /note="UDP-N-acetyl-Glucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlC"
                     /product="fnlC"
                     /protein_id="AXY99387.1"
                     /translation="MKKLKVVSVVGTRPEIIRLSRVLIKLDQFCEHIIVHTGQNYDYE
                     LNEVFFTDLGVRKPDHFLNAAGANAAETIGNVIIKVDQVLEKVSPEAVLILGDTNSCI
                     SAIPAKRRKIPIFHMEAGNRCFDQRVPEETNRRIVDHTADINLTYSDIARQYLLSEGI
                     PADRVIKTGSPMFEVLSHYMEQIDKSKVLSRLGLEPGAFFVVSAHREENVDSPKHLMK
                     LVNILNTVAEKYNLPVIVSTHPRTRKRIDDHGITFHNNIQLLKPLGFHDYNHLQKHAK
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEASVMMVGLECDRVLQALNILSLQ
                     PKGNERLLRQVADYSMPNVSDKVVRIIHSYTDYVKRVVWKEY"
     CDS             9825..11036
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99388.1"
                     /translation="MRLALIIDDYLPYSTRVGAKMFHELALELQSLGHHITIITPDKD
                     QPSNLYIDKLDDITIWRFKNGPIKDVKKIQRAINETLLSYNAWKAIKHLINKDSFDGI
                     IYYSPSIFWGKLVNKLKKKCQCPSYLILRDLFPQWIVDAGILKQGSLIEQYFRFFERY
                     SYKQASIIGLMSQKNLDYFYQHNKNKGLKCEILRNWANTTQTNIDTNGYISIRESLNL
                     QNKTIFFYGGNIGHAQDMGNLMRLVKKMSVYPDAHFLFLGQGDEVELIKTLSNKWNLN
                     NFSYLPSVDQTKFKLILKEIDIGLFSLASHHSAHNFPGKLLGYMANHIPILGSVNEGN
                     DLQELVNKENAGFIHINGEDDNLFESAKKLHLDIELRKKCGNDAYNLLCKEFSVTSIA
                     STLIGRLRSLK"
     CDS             11033..11434
                     /note="orf10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="hypothetical protein"
                     /protein_id="AXY99389.1"
                     /translation="MKYIDNSSLENLYKEAKKSERLRSHLLLHKSHEDKVQRLLIALV
                     KGSYVEPHYHELPHQWEMFIILEGQIEITLYNHSGEIKESFLAGENTNISIVNLEPNE
                     IHSLKCISQKALMLEVKEGPFDTKYAKKFLI"
     CDS             11496..12662
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99390.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             12674..13177
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99391.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13253..13810)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99392.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(13810..14631)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99393.1"
                     /translation="MSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHENLGSA
                     LSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIMLDHAT
                     GIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDGI"
     CDS             complement(14747..15763)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99394.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVQNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     STLF"
     gene            complement(15763..16014)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5473 a   2636 c   2652 g   5255 t
ORIGIN
        1 gtcaaggtgt tatgctagaa caacatatcg aagcggaatt agcgcaagat cccgctaacg
       61 atctcctttg gtggcgtcga ttaattcgtg ccattgataa atgggcccct cctggccagc
      121 gtttaattat cgtaaccagt gaaggacgta ttatcggcgc tcaacgtaat gaaatgcagg
      181 ttgtcagaaa ctttattggc caatctgata acgccgatca tcctaaaaag aaaaaatatg
      241 gtcgttctga gatgttaggg ccattttcag taagggatgg tgaagatcat tatcagcttt
      301 atcttgtacg tccatcaagt agccctcaat ctgactttat caacttatta tttgataaac
      361 cgttattgct actcatattc actatgctta ttagcacacc gttattagtg tggctttctt
      421 ggagcttggc aaaaccggca agacaactta aaaacgcagc tgatgatgtt gcgaaaggta
      481 atttacgccc tcatccagag ctagaaaccg gcccacaaga gtttttagct gctggtacta
      541 gttttaatca gatgataagc gcactcgaac gcatggttga agcacagcaa cgtttaattt
      601 ctgatatttc tcatgaatta cgaactccat taacacgctt acaattagcc agtgcattgt
      661 tacgtcgccg caacggagaa agtaaagaat tagaacgcat tgaaactgag actcaacgcc
      721 ttgatgggat gattaatgac ttattggtgc tttcacgtaa tcaatataaa aatgagttgt
      781 tacgagaaac tgtaaaagca aatgagttgt gggatgatat tttagataac gctaaatttg
      841 aggctgaaca gagtaataag acattacaag tgacaacacc tccaggtgca tggcctattt
      901 actgtaaccc ttactcactc gctagcgcat ttgaaaatat tgttcgtaat gccttacgtt
      961 attcaaattc tcgcattgaa gttgcattca cagagcaaaa tcaaggcatc accattattg
     1021 ctgatgacga tggcccaggt gttagccctg aagacagaga gcatatcttc cgaccgtttt
     1081 atcgcactga cgaagcaaga gatcgcgaat caggtggtac aggcttagga ttagcgattg
     1141 ttgagacagc agtaagtcaa catcgaggcc atgttaaagc cgatgacagc ccattaggtg
     1201 ggctgagagt agagatttgg ttgccgaggg gaagtgccgg taataaatag ccaatagcct
     1261 agatagagta attaaactgt tgtatcaaaa tatcctgcca tctattgaag aataggtggc
     1321 atattctttt tgttagcatc taatgatgca attcagaatg atctggctaa atcgaccgct
     1381 attccgatat ttatatatct acaatcaatg ctaaaatacc cagctattac ttgaccgtct
     1441 ggtttgaaga gtctactctc agtgcggttt ttcgcatatt gttaaaatgt gcttatataa
     1501 caaaactctt atattacaaa aagataaggg tttttcgaga taagttttgg cgctctgggg
     1561 gccgtaaacc cagtgcggta gctttggaat aaatataatt tatcacttat aagaaattct
     1621 acaacaaggt ctcatataaa attaattatt gttaataaat tgtcagcatt gcaacacaaa
     1681 atattattat aagaattatt aagttcagat ttttatattt acaatatatt aaataattac
     1741 catcctttcg tagagataac tataaccaaa ctataaaaat ttaattattc ttatttaata
     1801 atatatgatg gagagatttg tgttaaaaag tcaattgaat tattttaacc caacaataat
     1861 atttgtatta gggtggatgt ttttttgtct tttaccactt atctattttg gagaaccacc
     1921 tttattaata ataatgctaa atatagtaat tgttttctca tttataattg gctgtgtact
     1981 ttcttatagt agtaaaaatt ggataagtta tgttctatat gataataagt attcaagaat
     2041 taaatcttta ctttctatct tatgcttttt atatatcatt attaaacttt accttattag
     2101 cacaactgaa ataaactctg gtaattactc cgaaaaatat gctgttgaat cgcagttcta
     2161 tttatattta atgatatttg acaaattatt caatgttata tttttatttt tcttatcctt
     2221 gtttgctatt gaaggaaaaa taaaatttta ttttgttttc tcaatttctt tgttaagtac
     2281 tttctttgaa ccgactagat tattaattat attaacatta gtatattgga tccttttggg
     2341 tgtttccatt ggttatataa aaataagatt aattcaagtt atactaatag ccttagcatc
     2401 accctttata ttttctttct tattaataaa aagagtttat tcatttgaag ggagctctta
     2461 tgattttatt ttccatttat ttaaattaat agactatgat ttactagtct ctggaatgat
     2521 taatggttta gaaacttttg aatcttataa agcattaata aatgttataa atgataattt
     2581 tattcatcca ctatcaggtt ttgtccgcat cttatttatg cctattccta gaacattttg
     2641 gatagataaa ccagaatcga tagcaaggtt aattgctgaa aactattatc cagaagcata
     2701 tcaaaaaggt ggtggacaat tagcaggccc aataggagat ggttttataa atgcaggttt
     2761 tttaggtgta tttattattt ggttagctct aggctttatc tccgtttctt tttacaaata
     2821 ttttaaacgt ggtgtaatga aaggtggtaa tatagaaaaa gcctattact gtactttcta
     2881 ttatatatat ctaatctatt ttattaattg tttacgtgga tttgcttcag atttattttg
     2941 ggtttttgct gtgaatatga taatgcttta tattataaaa gtaatttttt tcaaacgaat
     3001 ccaatataaa gaataaatat ttattttaat aaatttaatt acttttaaat aaaaatagga
     3061 ataacaaaag tgaatctgat attttgtgga aacctacata atggcggtgg agtccaagtt
     3121 gccatatcat ttattcaaga acttgtaaca aataaaatta cagatttaag caatacagat
     3181 atcatcatat ctagcaaaat agaggaagaa ttagaaaaaa taaaaataaa tttaaatgag
     3241 tttaaaaatg cttatacaat gaatttttat gggcttagaa agaataaaga tttcaaagtt
     3301 attaataata aatataacat atgttttatc atatttgggc ctatttacta tagtttaaac
     3361 tcagaaaaat atattgttgg ttttgctcaa ccttggattg catataaaga taatgatgtt
     3421 tatcttaaaa tgtcgttaat atctaaaatt aaaaacaaga ttttctttac tgtaaaagat
     3481 ttaatttttc gcaagtataa acacttagtg gttgaacatg agcatgttaa aactgcactc
     3541 ttagagaaaa actacaagtc aaaaatagag gtcgtttcaa atacatactc atctattttc
     3601 gatgataaga attcttggga taaaatagac ttaccaaaag gattagataa ttcaaagttt
     3661 acttttggtt acattggtcg ttcttactta cataaaaacc ttaaaattct taaaaaggtt
     3721 aatcaaatcc ttttaagtaa atataatatt gatgtgaatt ttctatttac tttaagcaat
     3781 gaagaaatga aaatattgga atttgataac atacctaact tttataacac gggttgtatt
     3841 actctcaatc aatgtcctaa tttttataat aatatagatg ctctcatatt ccctaccatg
     3901 ttggagtgtt tttcagcaac acctattgaa gcgatgaaaa tgaacactcc tgtgttttgt
     3961 tctaattatc cattcttaac agaaatatgc aaaaatgcag cattatattt tgatgcaaca
     4021 tcaagtgaag atattgcaaa attaatcgca ggatcaatta ataataaaaa tttattagaa
     4081 gataaaatac aaaatggtct aaaagttgta aacactttac catcatcata tgatagagca
     4141 atgaagtata taaatttaat gaataatatt taattatcaa atgaaaaaaa acagtttttt
     4201 aaaatcaggt agttaccttc ttatttctgt aatgttcgct aaagcaatag catttatcct
     4261 taacattttg tgtgccagat ttcttgataa agaaagtttt ggtcagcttt cttttattag
     4321 gaatactgta gcaatgtttg aagggcttgt tagtggtagt aatggaaatt taataatttc
     4381 atcgcttgga aataaaaatt attattcatc aattatctta tctttttcct tttatatttc
     4441 catctcaata ttagtattta ttatattact aaagacctct tatggagggt ttatattaat
     4501 agataactat tcctttttat tgatgatcgt aatgttctta tcttctagct ttctaggaat
     4561 aattaattcc atatacattg cacatcaaga aaataaaatt atggttatat cttctttttt
     4621 ttctggcatt gtttcattta ttatttgtac tttattaatc tattatggaa gctatcacgg
     4681 tgcattatta gcttatgctg tttatttctc tatagacttt ttttttaaaa tttactttat
     4741 cttcaaaaaa gtcattaagt taaaatttac aagatttgtt tttgacactt ctattttaaa
     4801 gaggagcttt caattactta ttttcacaag tctatcattg tgcttctttt ggtatattag
     4861 aaataaactg gtaattgaaa aaaatgggct gtctgaactt gctgaatttg aattagtata
     4921 tcaatttgtt actatcgctt tattagcaat tggtgcattt acatcaacca tgttatcaaa
     4981 aattagtcat gaaaatggaa ttctaataaa aacaataata attagtttag tctttagcct
     5041 tactagctat ttagttgcat gcatatttgg tgaaacctta tttaaagcaa taaacccatt
     5101 atacaataat attggaaaac ttattccata tactgttttc attatatttc cctatactgc
     5161 aagttcaatt gtaaataaat tattaattat acataaatta gaatatataa gtgttcattc
     5221 ttcatttttt gccgtcttag tttgctcatg ttttttctta ttggataaac cattaagtgc
     5281 aatgacatta tcaatattat attgtgttta ttatttctta gtcacatcaa tatcactatc
     5341 ttactttttc ctattgaaaa aagtaagacc taattattag taataacatt tattataaaa
     5401 atatagtatt ctttatatat taataatctt tctcactgag aaaaaattca ttagaaaatg
     5461 catatattat gatgaaaaaa aaagttttaa tccttcaacc cgtcatacct aattatagat
     5521 tagatttttt tgataaatta tataatgatg aatcaattga tctaaaaata aaagcatcca
     5581 atgaagatat gattggtgta aaaacctgct cagaagcaaa agaaagagaa tatattgagt
     5641 taattggcaa ttttatgtca ttctttaatt tatttttttg gcaacgtgga ttaagattga
     5701 aatctatatt atgggcagat gttgttgtta tttctggaaa tcctcggtta ctcaatcatg
     5761 ttctattttt attcttttgt aaaattacta aaaggaaagt tgtttggtgg gggcaagggt
     5821 ggactgctgg aagtcatggg ctaaatgcaa aaataagacg acagttaatg agatttgctg
     5881 atgctctagt actttataca gaaaatgaag caatggaatt ttcaaatcat aaattagcta
     5941 ttggtttaaa taatggccta aatactaaaa aaataagaaa agaattatca acagttatat
     6001 cacaaagaga tgaaaatagt gatgttatac atctatgttt tattggcaga cttactcaaa
     6061 aaagtaagtt agatatactt ttatcttcat taagagatgt agataggaaa gtaaaattaa
     6121 gtattattgg tgatggtata ctaagaagtc aacttgaaaa gtttgctcat gataatttaa
     6181 aattaaaaaa taatatagat attcaattct atggcgctat atataatgaa catgatattg
     6241 gagaaataac acaagtatct gatgctttta tttaccccgg tgctgtaggt ctaagtctta
     6301 ttcacgcata ctgtcatgga cttcctgctt tagttcatgg tgatagtaac aatcatatgc
     6361 ctgaatatgc cgcatttaaa ttaggaataa atggtttaaa tattcctcag aaaagcaaag
     6421 acctatccaa gtttattaat tcaattgact tgcttcaatt aagaaatatg agagatgcag
     6481 ctataaatac tgtcataaac acttttaata ctgatgacat ggccaagcgc tttgtttcga
     6541 taataaaaaa ggattaaaaa aaatgtttaa agataaaacg ttactaatca ctggcggtac
     6601 aggctccttt ggcaatgctg tcttaaatcg ctttttagaa acagatataa aagaaattcg
     6661 tatattttct cgcgatgaaa aaaaacaaga tgatatgcgt aaaaaatata ataatcataa
     6721 attaaagttt tatattggcg atgttcgtga ttattcaagt attttaaatg caactcgtgg
     6781 cgttgatttt atttttcatg cggccgcact taaacaagta ccttcatgtg aatttcatcc
     6841 aattcaagcc gttaaaacaa atattttggg tacggaaaat gttttagaag ctgcgatagc
     6901 taatagtgta aaaagagtcg tttgtttgag cacagataaa gcggtgtatc cgatcaatgc
     6961 aatgggtata tccaaagcaa tgatggaaaa agtcatggtt gctaaatctc gtaatttaga
     7021 tagcaataaa accgttattt gtggaacacg ttacggtaat gtaatggctt cacgaggttc
     7081 agttattcct ttatttgtcg atttaatgag tgaaggtaaa gctattacaa ttacagatcc
     7141 taatatgact cgctttatga tgacattaga agatgccgtt gatcttgtat tatatgcatt
     7201 ccaaaacgga aataatggcg atatttttgt tcaaaaagca ccagcagcaa caattgaaac
     7261 attagcaata gcattaaaag aactcttgaa tacaccagaa cacccaataa atattattgg
     7321 tacgcgccat ggggaaaaac tttacgaagc tcttttaagt agagaagaaa tgttagcagc
     7381 tattgatatg ggtgattatt accgtgtccc accagatttg cgtgatctta attatgataa
     7441 atatgttgaa caaggcgatt cacgtatttc tgaaattgag gactataatt cacataatac
     7501 gactagatta gatgtaaatg aaatgaaaaa gctattatta aaattgcctt taataaaaaa
     7561 cttacacaat aataaaattt aaggtcattt gaatatgaaa atactagtta ctggtgctga
     7621 tggttttatt ggtaaaaatt tatgtgttcg attagcagaa aataatttcg aattaataaa
     7681 aatagaccgt aatagttcaa aacatgactt agaactaggc ctgatgaatg caaactttat
     7741 ctttcattta gctggaataa atagacctaa aaatgaatca gagtttcatg aaggtaatgc
     7801 atcattaact gaagaaatca cgcatttcct gattgaaaat aagaaaaaaa caccaataat
     7861 gctgagttca tcaattcaag caaataataa taataaatat ggtgaaagca agaaattagc
     7921 agaaactcat ataaaagcat atgggttaaa aactcatgca aactattata tttatagatt
     7981 gccgaatgtt tttggtaaat ggtgtaaacc aaattacaac tcgtttattg caaccttttg
     8041 ccataatatt gcaaatgata ttgatattat cataaactcc ccaacagcca aagtttcact
     8101 aatttatatt gacgatttat gtaatgaatt aatctctcta ttaaatggta ataatagaat
     8161 tggctttcaa aatatttcac ctatttatac aactaccgtt ggtgaggttg ctgaattact
     8221 ttatgccttt aaaaatagtc gcaatacatt agttacagaa aatgtaggtg ttggttttac
     8281 tcgagcacta tattccactt ggctaagcta tctttcacct cagcaattta cttattcagt
     8341 tcctcattat gaagatcaaa gaggtgtttt ttgtgaaatg cttaaaacac aaaacagtgg
     8401 ccaattttct ttttttaccg cacaccctgg tattactcgt gggggacatt atcaccatac
     8461 taaaacagaa aaattcttaa ttattaaagg aaaagctttg tttaaatttg agaatattat
     8521 tacaggtgaa aaatacgagt taactgtttc tgaccatgat tataaaatcg tagaaacagt
     8581 accaggatgg tctcatgaca ttacaaatat tgccgatagt gaattaattg ttatgttatg
     8641 ggcaaatgaa atctttaata aagaatttcc agatacaata gcgagacctt tgtaatgaaa
     8701 aaattaaaag ttgtatccgt tgttggtact cgtccagaaa ttattcgtct atctagagtt
     8761 ttaataaaat tagatcaatt ttgtgaacat attattgttc atactggaca aaattacgac
     8821 tacgaactaa atgaagtttt tttcacagat ttaggtgtta gaaaacccga tcatttctta
     8881 aatgctgctg gcgctaatgc tgcagaaacg attggtaatg tgattataaa agttgatcaa
     8941 gtcttagaaa aagtctctcc agaagctgta cttattttag gtgatacaaa ttcttgcatt
     9001 tcagcaattc cagccaaaag acgtaaaata cctatttttc atatggaagc aggtaatcgg
     9061 tgttttgatc agcgcgtacc ggaggaaaca aataggcgta tcgttgatca tactgctgat
     9121 atcaatttga cctatagtga cattgctcgt caatatcttc tatcagaagg catacctgca
     9181 gatagagtta taaaaacagg tagtcctatg tttgaagtac tttctcatta tatggaacaa
     9241 attgataaat ctaaggtatt atctcgatta ggtttagaac ctggagcttt ttttgttgta
     9301 agtgctcacc gagaggaaaa tgttgactcg cctaagcatt taatgaaatt agtcaacatt
     9361 ttaaatactg ttgctgaaaa atataattta ccagttatcg tgtcgacaca tccaagaact
     9421 agaaaaagaa ttgatgacca tggaattact ttccataata atattcaatt attaaaacca
     9481 ttaggttttc atgattataa tcatctacaa aaacatgcaa aagcagttct gtctgacagt
     9541 ggaactatca cagaagaatc atcaattatg aacttcccgg ctcttaatat cagggaagct
     9601 cacgaaaggc cagaaggctt tgaggaagca tcagtgatga tggtcggttt agaatgtgat
     9661 cgagtgcttc aagcattaaa tatactctcc ttacaaccaa agggtaatga acgtttatta
     9721 cgccaagtag ctgattatag tatgcctaat gtttcagaca aagttgtaag aataattcat
     9781 tcttatactg actatgtgaa aagagttgtc tggaaagaat actaatgcga ttagctctta
     9841 tcattgacga ttacttgccc tacagcactc gagttggggc aaaaatgttt catgaattag
     9901 cattagagct acaatcactc ggacaccata ttacaatcat aactccagat aaggatcaac
     9961 catctaatct atatattgat aaattagatg atattactat ctggcgtttt aaaaatggtc
    10021 ctattaagga tgtaaaaaag atccaaagag caattaatga aacactactt tcttacaatg
    10081 cttggaaagc tattaaacat ttaatcaata aggattcttt tgatggcata atttactatt
    10141 ctccttctat attttgggga aaattagtta ataaactaaa gaaaaaatgc cagtgtcctt
    10201 cttatttaat attaagggat ttatttcctc aatggattgt tgatgctggt attttaaagc
    10261 aaggttcatt gatcgaacag tactttcgat tttttgaacg ttactcctat aaacaagcat
    10321 caataattgg cttaatgtct caaaaaaatc tagattattt ttatcaacat aataaaaata
    10381 aaggtttgaa atgtgaaatt ttaagaaatt gggcaaatac tactcaaaca aacattgata
    10441 ctaatggata tatttcaata cgtgaatctt taaacttaca aaataaaact attttctttt
    10501 atggtggaaa cattggtcat gcccaagata tgggtaattt gatgcgacta gtgaagaaaa
    10561 tgagtgttta tcctgatgct cattttctct ttcttggaca aggcgatgaa gttgaattaa
    10621 ttaaaacttt atctaataaa tggaatttaa ataattttag ctatctacct tcagttgatc
    10681 agactaaatt taaattaatt cttaaagaaa ttgatattgg tttattttca ttagcgtcac
    10741 atcactctgc acataatttt ccaggtaaat tattaggata tatggctaat catatcccta
    10801 tattaggtag cgttaatgaa ggaaacgact tacaagaact agtaaataaa gaaaatgcag
    10861 gttttattca tataaatggc gaagacgata atttatttga atctgctaaa aagctccacc
    10921 ttgatataga actacgaaaa aaatgtggaa atgatgctta taatctgttg tgtaaagagt
    10981 tttcagttac ttctattgca tcgacactaa taggtaggtt gagatcttta aaatgaaata
    11041 tattgataat tcatcgttag agaatttata taaagaagca aaaaaatcag aaaggttacg
    11101 ttcccattta ttattacata aatcccatga agacaaagta caacgcctcc ttattgcttt
    11161 agtaaaaggt agctatgttg aaccgcatta tcacgagtta cctcatcaat gggaaatgtt
    11221 tattatttta gaaggacaaa ttgaaatcac actatataac cattctggag aaataaaaga
    11281 aagtttcttg gctggagaaa atactaatat ttcgattgta aacttagaac caaatgaaat
    11341 acacagtctt aaatgtattt ctcaaaaagc attaatgttg gaagtaaaag aaggcccttt
    11401 tgacaccaaa tatgctaaaa aattcttaat ttaatagagc aaattactat ctataatccc
    11461 tcctataaaa ttagattact catcaaggct tatttatgaa aatcaccatc tcaggtaccg
    11521 gctatgtcgg tctatctaac ggaatactcc tttcccaaaa caacaaagtt gttgcgttag
    11581 atatcaataa agaaaaagtt gaattactga ataaaaaaat ctctccaatt acagataaag
    11641 aaattgaaga atttttagca accaaagagc tcgattttac tgcaacctta gataaagagt
    11701 tcgcttataa agatgcggat tttgttatca ttgcaacacc taccgattac gatcctaaaa
    11761 caaattactt taatacctcc tctgtagagt ctgttatccg tgatgtttta gaatataatc
    11821 caaacacgac aatgattata aaatcaacca ttcctgttgg ttttacgaaa gaaatgagag
    11881 aaaaatataa tacggataat attattttct ctcctgaatt cttacgtgaa ggtcgtgcac
    11941 tttacgataa tttataccca tcgcgtattg ttattggtga acaatcagaa agagcacaaa
    12001 aatttgcaga ccttttacaa caaggtgcat taaagaaaga tattgatgtt ctgttcactg
    12061 actcaacaga agcagaagcc attaaattat ttgccaatac ttatttagca ctacgtgttg
    12121 cttactttaa tgaattagat agctacgctc aagcttatgg cttaaatgca aaacaaatca
    12181 tcgaaggcgt ctgcttagat ccacgtatcg gtaatcatta taataaccca tcattcggtt
    12241 atggcggtta ctgcttacca aaagatacta aacagctgtt agcaaactac gactcggtgc
    12301 ctaataatat tattagtgca atcgtcgaag ctaaccgcac acgtaaagac tttattgccg
    12361 actcaattat cgctaaatca ccaaaaatcg taggtgttta tcgtttaatt atgaaatctg
    12421 gctctgataa tttccgttct tcttctatcc aaggcattat gaaacgcatt aaagccaaag
    12481 ggattgaggt ggtaatctat gagccagaga tgaaagaaga gactttcttt aactcaaaat
    12541 taattcgtga tttaaatgag tttaaagcaa tctctgatgt cattattact aatagaatgt
    12601 catctgtact aaacgacgtt gaagaaaaag tttatactcg tgacctcttt ggtaacgact
    12661 aaatcgattt tatatgctca atatcgtctt atttgaaccc gaaattccac cgaatacggg
    12721 taatattatc cgcctgtgtg ctaacacagg ctttcatctt catttgatcc aacctttggg
    12781 ttttacttgg gatgataaac gtcttcgtcg tgcaggactt gattatcgtg aatttgctga
    12841 tattaaacag catcacgatt actatgcttt tttagaaagt gaaggtttaa accctgataa
    12901 tgcacaatca ccaacgggtg cgcgtgtatt tgcgttaacg acaaaaggta caccagcaca
    12961 tagcaatgta agctatcaag atggagatta tctccttttt ggccctgaaa cccgtggttt
    13021 gccaccttat gtgttagata atatgccaac acagcagaaa atcagagttc caatgcttgc
    13081 tgatagtcgc agtatgaacc tgtcaaatac cgttgcagtt gtggtttttg aagcatggcg
    13141 tcaacttggt tatcctggtg cgttattaag agactgaaat gcaaaaagcg ctgatcataa
    13201 ataatgagca agcgcttttt taatttcaaa tgactaaaaa cttaatttat tattacattg
    13261 agagttgttt tatttcagca acaaccttcg tgagatacct acacccttcc cctacatcct
    13321 tcaataccaa tgcgtgagca ccaatcactg cattatcacc aatctctatc tcaccgccga
    13381 taatcgaaga attacaacca atataaacgt ggttgccgac tcgaatatct gctttaccat
    13441 ccttttcttt tactccgatg gtcacacctt gcattaacgt cacattctca cctaattgac
    13501 agtaattagt cagcacaata ccgataggat gtggtaatga aaggcctttt cctatgcgcg
    13561 ttcttaatga gatatcattg caatatttac gattgttttt agcaccaatg taaatgccta
    13621 attttttgag aaaacgattt ttagagaaat aaaaaaattg agcgacacgc caccaaaaca
    13681 gataacgtga acgttggcag aaaaagacgc gatgaaaaag tcttatccaa ctaaaaggct
    13741 tgttatcacc gatatattca gcgtgtaaac aagccttaag ttcagaaaat gtaacttctg
    13801 ttaatgccat taaatgccgt caccacattc aaaaccaggt actacaccat taaagtgttg
    13861 atccatatcg agtgaaggtt tatcactcgt cggcttacca acaatgcgag caggaacccc
    13921 tgcaaccgtg gtatgtgcag gcactgcatg taatacgaca gatcccgcac caattttagc
    13981 accgcgacca atctcaatat tgccgagtat tttagcaccc gcaccaatca tcacaccttc
    14041 acgcacttta ggatgacgat caccacaggt tttacctgtt ccgcctaaag tcacagattg
    14101 taaaatagaa acatcattct caacaacagc tgtctcacca attacaatgc ctgttgcgtg
    14161 atcaagcata ataccgtggc caatacgtgc agctgggtga atatcgacac caaacgtaac
    14221 agaaatctgg ttttgtaggt aaacagcaag cgctttgcga tcttgtcccc ataaccaatg
    14281 tgctatgcga taagcctgca acgcatgaaa gccttttaaa taaagcaacg gtgtggagta
    14341 tttatcaact gcggggtcgc gtaaacgaac agctgaaata tcataagccg cagattcgat
    14401 catggaatca tcgctacgat acgcttcttc aacaatctca cgaacggcaa tcgcaggcat
    14461 tgtctgtgtc gccagcttat tcgctagcat ataacttaag gcgctaccta agttttcatg
    14521 tttgagtaat gtcgcatgga agaaactcgc caaaatcggc tcgcaatcag ccaaacatct
    14581 tgcttcattt ttaatatgat cccagactct ttttagttct tctagcgaca tactcactct
    14641 cattcttttt acattgaaac ctaagtaaca acttaagctt cttatgttga aaaccgaaac
    14701 agacacctta aaccaatgcg ttaagtagcg attatttaaa atagacttag aacagagtgc
    14761 tgtctatttc atcttttgtt gcgcgaccta ataaagatag tgccgcctct ttcgcgtctt
    14821 tatgttggta taaaatttgg tagatctgct cagtgatagg catttcaaca ccgacttgtt
    14881 ctgctaaggc gcgaacttct ttagtgttcc gataaccttc aaccacctgc ccgattttct
    14941 cttgggcagt atcaacatca agcccttggc ctaacatcat accaaatcgg cgattacggg
    15001 attggttatc ggtacaagtt aaaacgagat cgcctaaccc ggccattccc ataaacgtcg
    15061 cagcatctgc acctaatgct ttacctaaac ggctcatttc agcaagtccg cgggtaatta
    15121 gtgctgtacg tgcattcgcg ccaaacccca taccatcaga cattccagca ccaatagcaa
    15181 tcacgttttg taccgcgcca cctaattgca caccgataaa gtcaggattt ttatacacac
    15241 ggaaactttt accacaatgg aacaactgtt gaagctcttc agaaaacgta ttatctgttg
    15301 atgcaacaga tatcgcagta ggcagacctg ctgcaagctc tttggcaaaa gtaggaccag
    15361 ataaaactgc gagcgggatt tcattaccta atacttcacg cgccacatct tgtaacaagc
    15421 gaccagtatg agcctcaagt ccttttgtcg cccaaacaac acgtgcatcc tgacgtaaaa
    15481 agggtttgat ctgctgtaat acgtcaccaa aaacatggct tgggatcaca accagaatat
    15541 tacggctagc ttcaatggct ttttgcaaag aagcttccat gtataaacta tcaggaaaag
    15601 aaacatcggg cagaaatgcc tgattacaac gtgcttgttc taacgccgca acatgttcag
    15661 catcatgacc ccaaagtaca acatcatggc cattgcgtgc taaggtaatc gctaaagcgg
    15721 tgccgtatga accggcaccg ataactgtca tagaagcgtt cattaagctt cttcagcccc
    15781 ttcattagca gcatcagttt gttgctgttg taaatagttc ataaacaacg catcaaagtt
    15841 aactggtgct aagttcagtt gtgggaaagt accacggctg actaagttag tgatacattc
    15901 acgagcataa gggaacagaa cgttagggca atatgcgcct aagcaatgtg ctaattgagt
    15961 gccttcaata ccttcaacag taaagatacc cgcttgttgt acttcacaca gggggt
//
