LOCUS       KY710690               16910 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 18/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710690
VERSION     KY710690.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16910)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 18/57"
                     /serotype="O9"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..1265
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16910
                     /note="O antigen gene cluster"
     CDS             1576..2676
                     /note="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="vioA"
                     /product="vioA"
                     /protein_id="AXY99395.1"
                     /translation="METITVTSPLFPPLEELEPYLQDIWKRKWLTNNGYYHQRLEEAL
                     CKYLNVPYISLYSNGTLALIAAINVLKLKGEVITTPYSFVATSHSLLHSHITPVFVDI
                     EPNTYNIDPDKIEAAITDKTTAILPVHVYGRPCNHEAIQKIADKYNLPVIYDAAHAFG
                     VKENGNSILNQGNLSILSFHATKTYSTIEGGAIICHSAEMKKKLDKYKNFGYTNETEI
                     DELGFNAKLNEVQSAFGLLTLNYIDKAIEKRKEVSDFYKTNLSNVAGITLPNIPKDLT
                     WNYSYYPIIIDKDRYGKTRDELYLELKENKILSRRYFYPLITDFNIYNQYKTKTPTAE
                     KISENVICLPIHHLLNNDDLNRIINIIKYDYK"
     CDS             2663..3715
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99396.1"
                     /translation="MTINKLINPLPEKEIIKKWNSEEIFVSVICCTYNQEKYIEDTIL
                     GVLCQDTPYRYEFIIHDDCSTDSTSDIIKKYADNYPKIIKHIIPAKNIYSQGIQPGIN
                     AFSFSNGKYIALCEGDDFWIDEKKLTKQVDILENKKDINICFSPAYTLQPNGKIDLTC
                     NYHNQIFCAPLSTVIRQGGAYMPTASLLFRRDVFIQFKNNFSKASIGDYYCQILGSIN
                     KGAIYLPDITCVYRINSLGSWSKSRINVSKEILISEIDEYERNLYKLIILGADKKDID
                     YSLADISRVNANELFLRKDWSGCKSLIIKSWLFNKKINRNQEKLYLYRNILPILYLRE
                     KIKKSLKKISLKRIKL"
     CDS             3715..4407
                     /codon_start=1
                     /transl_table=11
                     /gene="wbqC"
                     /product="wbqC"
                     /protein_id="AXY99397.1"
                     /translation="MRLAVMQPYLFPYIGYYQLAYYADKFIFYDDVNYIKGGYINRNN
                     ILTKNGLQMFTLPVNKASSFKKINELYFQDNTKKILTSIYQNYSKTAYFHSIFPIIEN
                     VLTNNNRNVANITSLSIIKIFEYLSLKFNYTFSSDQNYNRTYNAQEKIYELCKINDAQ
                     EYINPIGGKDLYSKNEFKKRNIKLNFLEPILSPYTQINSSNFVSNTSVIDILMNCNKD
                     EIINQFNNFKII"
     CDS             4416..4982
                     /note="galactoside O-acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="vioB"
                     /product="vioB"
                     /protein_id="AXY99398.1"
                     /translation="MSYYTTEQLAEIGFKKIGTNCKISTKASIYRPEEIEIGNDCRID
                     DFVILSGKIHLKNNIHIAAFCNLSAGECSILFEDFSGLAYGCHVFTGSDDYSGRTMTN
                     PTIPLKYKNLKEKNIIIGKHAIIGTNSIIMPGVTIAEGTAVGALSMVTKSTKPWGIYF
                     GNPAKRIKERKKDLLNLEMEYIKEKSKK"
     CDS             5562..6170
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="hypothetical protein"
                     /protein_id="AXY99399.1"
                     /translation="MKKILLLDICGTITSKNTTIDFINYIGFKPSHIKLLIGKIMWKF
                     FHNDILRRWYLLELKGIDKKELQTLAEKYIAQLKFDKEILKYISYLQENGYDIYIISA
                     TLDCIANEISKIVNSKRIYSSELSFKNNVCCGSLKKDLLDKKLIELYKDNIFYNNITL
                     TISDNEGDLDVMKHSNYSIAVVRNKKKQSFWKKKNILSLRRY"
     CDS             6172..6951
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99400.1"
                     /translation="MNFFYIPIIYTFKTRLKNLVSLFSWFIICPLLLFILLITKEQGS
                     ITLSIIICYILSFIHTYNLYEIGYIINDTETIKKESNPTLRLSDSKLSFYYKKRLSIY
                     LERIILSLFISIILIYYYNTPLIAISYSWLLIPFYLIYNNIRNIYNLPIHFILVVLRY
                     TSPLILTINLNPEDFFYIILVFPLVNLIERASEKRFNINFLIKRRNKIRNLRIFYYFI
                     ILLIFITLDILNYNIPSYIIIASFYMLLYRILSPIIINKTK"
     CDS             6948..9431
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99401.1"
                     /translation="MTNYIEIYTILSLIICSFLILTYDFYKSPKNVLLNLSSYSLLYS
                     IFYYGVPFLNIEYLFEYFDYSISNSTLIITRFYSIWTLFIFLALYLLIRNNNSTLIDL
                     KKRNYSPPNIFIITIIIFLLTLSIYILSKVGSDLIANFSNRVSAYTIYVQYKVKYKLP
                     ILFIFLLYCIYTLKVNRNQKINFIYYIVPLIFMTPELLSGGRGMLFTYILFLFVLSIL
                     KLKDNTIKKAFLFIASLLFIMVFIRYLLTRNINENTFEYTIYSFFAEFYHTAFTTPYI
                     LEHNIFSYTDSIKYIIYPFSKFFTPITNYLDINTQLPWYADIVSEHINRHFGFAGNII
                     SEALYYWGPIAPIIYPLIISLIIIIHNNYKNSITGFFFFIIMAINLRLFFRGSFWDSY
                     TSILIWFFIFYFLLLAFSLKRKNEYKYKMNQITSSMLRILTLIAKFLFSIYLTKYYNI
                     TVVGIYGIISSIVSLLIYLSGLELYSILTPFYNKNSKLDSFLFSNQMFFSIISTLSLA
                     FFILPVIYSYYNSYIQIFLFTIIIITVEIFQQEIYRILLIRGKALVANIAYFIRNGLW
                     CYVFILYSYLLKIDVQNITTAFLSIWGGVGLLGCIFSYIMVRKEKIYFKYNNIRKRFI
                     FLNIKKAFPIFLSSIFMRIIITSDKMILGLLTNLYQVGIYTFFISISNAFQALIDAGA
                     VSHKYKEIINSSKNYDYLKLKKLFIYIGKTSICLYVITLTSTTLFFNFFNFHDYLENI
                     LCLSIILFSSIFFSFGNITKLVLVSQRKKKEILYPSILSINIFFFIILFNCLLKNSMV
                     IMSVSLLLCSIAYVILSFKYIYINRSYSE"
     CDS             9424..10476
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99402.1"
                     /translation="MNNQVTILINSLVQGGAEKVSLRISDIFEEYGITNNYVIVSNNI
                     FYNTNKKITCLSNKPKVSKLKKIFLLKRFFKESNTVLCFSLDLACYLILLRCLGQFKG
                     KIICRFINNPNNEVHGFTSKIKKKILFSILKKSDFIICQSLNMQKILLNDYSFNKEKT
                     ITIYNPIEFNNNKITQKNKSTINLLFIGRLTHQKNLFDIIKISLELTKKNINFLWNIV
                     GDGEEYNNFKTLIKKNNLSNYIHLLGAKKNVSQYYEEADITTLVSHYEGMPNVLLESI
                     AHGTPVISYDCISGPSEIIQENINGFLIPIYDTKKFADKIMDIEKIKKLKSKDLYNTI
                     PKFHPDIIIKEYKKIL"
     CDS             10764..11303
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99403.1"
                     /translation="MDTDDIAIPSRFKIQLSYFSKDPDLAVLGSNIDEFIISTSETTG
                     TRKVPSEYNDIKKYALFKCPLNHMTVMFRKKIVQDVGSYIHHHYMEDYNLWLRILASN
                     HKILNVEQSLVYARVNKNTLLRRRGLSYIKSEYKLAKLKKSLKLQKRVSSILTFILRA
                     SPRLLPPFILKLIYKINRD"
     CDS             11306..12472
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99404.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             12498..13508
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99405.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEAEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             13512..14015
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99406.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(14145..14699)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXY99407.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKEGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGERCRYITKVIADIRQI"
     CDS             complement(14702..15535)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99408.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15635..16648)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99409.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(16660..16902)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5997 a   2740 c   2585 g   5588 t
ORIGIN
        1 ggaccccagg tgttatgcta gagcaacata tagaagccga actagcgcaa gatccggcta
       61 acgatctgct atggtggagg cgattaatcc gcgccattga taaatgggcg ccacctggtc
      121 aacgtcttat tattgtcacc agtgaaggtc gtattattgg tgctcaacgt aatgaaatac
      181 aagttgtcag aaactttatg ggacaatctg ataacgcaga ccatccgaaa aagaaaaaat
      241 acggtcgctc tgagatgtta ggcccctttt ccattagaga tggtgaagat cactaccaac
      301 tttacttagt gcgcccatca agtagcccac aatctgactt tatcaattta ttatttgata
      361 aaccgctttt attactgata ttcaccatgc ttatcagtac accgctactg gtttggctct
      421 cttggagctt ggcaaaaccg gcacgaaaac tcaaaaatgc ggcggatgat gtggcaaaag
      481 gtaatctgcg ccctcaccct gagctggaaa ccggtccaca agaattttta gcggcaggca
      541 ccagttttaa tcagatgatc agcgccttag aacgcatggt tgaagcgcaa cagcgattga
      601 tttcggatat ctcccatgag ttgcgcaccc cattaactcg tttacagctc gccagtgcat
      661 tactacgtcg tcgtagcggt gaaagtaaag agctagagcg tattgaaaca gaaacacagc
      721 ggctagatgg catgatcaat gacttattag tgctttcccg caatcaatat aaaaatgagt
      781 tattacgcga aaccgtaaaa gcgaatgagc tttggaacga tattttagat aatgcgaaat
      841 ttgaggctga acagagcaat aaaactctaa cagtaaccgc cccgccaggc ccatggacga
      901 tttattgtaa tccttattca ttagcgagtg cctttgaaaa tatcgttcgc aatgccttgc
      961 gttattcaca ttctcgtatt gaagtggcct ttacagaaca aaatcagggt atcactatta
     1021 ttgttgatga tgatggccca ggggttagcc cagaagatcg cgagcatatt ttccgacctt
     1081 tttatcgtac ggacgaagca cgtgatagag aatccggcgg tacaggactg ggactggcaa
     1141 tagttgaaac cgcaattagt caacatcgag gccatgttaa agctgacgat agcccattag
     1201 ggggattaag agtggagatt tggttgccga gagggagtgt ggtaaataag tcaaataata
     1261 attaactgta ccataagcca ctaagttaaa acttttacct tgtttttatg cagtggctta
     1321 ttatttttta gtaaataaat ctataataac agtagttaaa ctcattagct cctttcgcat
     1381 aatacaaaca aaaatattaa tgcattttaa caccttattt attaatcctt ttttgaaagt
     1441 cactcctcat atttttatat cctagtcttt aagattaata tgtttagatg atgtataata
     1501 ctagtaatat tataaactct gggtactttc ccagtgcggt agctatgcct attggctttt
     1561 agagaaaata cttatatgga aacaatcaca gtaactagcc cattatttcc accgttggaa
     1621 gagctagaac cttacttaca agatatatgg aagagaaaat ggctaaccaa taatggttac
     1681 tatcatcaac gtcttgaaga ggcactatgt aaatacctaa atgtacctta cattagttta
     1741 tattcaaacg gaacattagc gctaattgca gcaataaacg tcttaaagct gaaaggtgaa
     1801 gtaatcacaa cgccatatag ctttgtcgca actagtcatt cattactaca tagtcatatt
     1861 acgcctgtct ttgtagatat agagccaaat acctataata tagatcccga taaaattgaa
     1921 gcagcaatta ccgataagac aaccgcgatt ttacctgttc atgtatatgg tcgcccatgt
     1981 aatcatgaag cgattcaaaa aattgcagat aagtataacc ttcccgtgat ttatgacgca
     2041 gcccacgctt tcggggtaaa agaaaatggt aactccatat taaatcaagg taacttatct
     2101 attttaagtt ttcacgcgac taagacttat tctactattg aaggcggtgc gattatttgc
     2161 catagcgcag agatgaaaaa aaaattagat aaatataaaa attttggtta cacaaatgaa
     2221 acagaaatcg atgagctagg attcaatgcg aaacttaatg aagtacaatc agcatttggt
     2281 ttgctaacat taaactatat agataaagca atagaaaaaa gaaaagaagt ttctgatttt
     2341 tataaaacta atctaagcaa tgtagctggt attacattac ctaatatacc aaaagattta
     2401 acttggaatt attcttacta tcctataata atagataaag acaggtatgg taaaacaaga
     2461 gatgaacttt atttagagct aaaagaaaat aaaatattaa gccgtagata tttttatcca
     2521 ttaataactg attttaatat ttataatcaa tataaaacga aaacccctac cgctgaaaaa
     2581 atatctgaaa atgttatttg tttaccaatc catcatttat taaataatga tgacttaaat
     2641 agaataatta atattattaa atatgactat aaataaacta attaatccct tgccagaaaa
     2701 agaaataata aaaaaatgga attctgaaga aattttcgta agtgtgatat gttgtacata
     2761 taaccaagaa aaatatatag aagatacaat acttggggta ctctgccaag atacgcctta
     2821 tagatatgaa tttattatac atgatgattg ttctacagat tctacatcag acataataaa
     2881 aaaatatgca gataattatc cgaaaataat aaaacatatt attcctgcta agaatattta
     2941 ttcacaagga attcagcctg gaattaatgc tttttctttt tctaatggta aatacattgc
     3001 tttatgtgaa ggagatgatt tttggataga tgagaagaaa ttaacaaagc aggtcgatat
     3061 attagaaaat aaaaaagata tcaacatttg ttttagccca gcttatacac ttcaacctaa
     3121 tggaaaaatt gatctcacat gtaactatca taaccagatc ttctgtgctc ctctttccac
     3181 tgttatccgt caaggtgggg catatatgcc aaccgcatct cttcttttca gaagagatgt
     3241 atttatacag tttaaaaata atttttcaaa agcttcgatt ggtgattatt attgccaaat
     3301 attaggtagt ataaataagg gtgctattta tttacctgat attacatgtg tatatcgtat
     3361 caattcatta ggctcttggt ctaaaagtag gataaatgtc agtaaagaaa ttttaatctc
     3421 tgaaatagat gaatatgaaa gaaatcttta taaattaatt atattaggtg ctgataaaaa
     3481 agatatcgat tattcgttag cagatatctc tagggtaaat gcaaatgaat tatttcttag
     3541 aaaagattgg agcggttgta aatctctaat tattaaatcc tggcttttta ataaaaaaat
     3601 aaatagaaat caagaaaaac tatatttata tagaaatata ttacccatct tatatttaag
     3661 agaaaaaata aaaaaatcac taaaaaaaat cagtctaaaa aggattaagt tataatgaga
     3721 ttagcagtaa tgcaacctta ccttttccca tatattggtt attatcaact agcttactat
     3781 gctgataaat ttatatttta tgatgatgtt aactatatca aaggaggata tataaacaga
     3841 aacaatatat tgactaaaaa tggattacag atgtttactc ttcctgtgaa caaagcatct
     3901 agctttaaga agataaatga actttacttt caagataata ctaaaaaaat attaacatca
     3961 atatatcaga attattctaa aacagcctat tttcattcta tttttcctat aattgaaaat
     4021 gtcttaacca ataataatag gaatgttgca aatatcacat cattatcaat aattaagata
     4081 tttgaatacc tttcattaaa atttaattat acattctcat cagatcaaaa ctataacaga
     4141 acgtataacg cccaagagaa aatatatgag ctatgcaaaa taaatgatgc tcaggaatat
     4201 ataaatccaa ttggtggaaa agacctttac agcaaaaatg aatttaaaaa aagaaatata
     4261 aaattaaatt tcttagaacc tatactatct ccctatactc aaataaattc atctaatttt
     4321 gtaagtaaca catcagtgat tgatattcta atgaattgta ataaagatga aataataaat
     4381 caatttaaca attttaaaat tatataggtc taagtatgtc atactataca acagaacaat
     4441 tagcagaaat tggttttaag aagattggaa caaattgtaa gataagtact aaggcatcta
     4501 tatatcgacc agaagaaatt gaaataggga atgattgtcg tattgatgac tttgttattt
     4561 tatcaggaaa aatacactta aaaaataata ttcacattgc agcattttgt aatttaagtg
     4621 caggtgaatg tagcatccta tttgaagatt tttcagggct agcctatgga tgccatgtct
     4681 ttacaggcag tgatgactat agcggtagaa caatgacaaa tccaacaatt cccctgaaat
     4741 ataaaaatct aaaagaaaaa aatattatta ttggaaagca tgcaatcatc ggaacgaact
     4801 caatcattat gcctggcgtt accatagcag aaggaaccgc tgtaggcgct ctatccatgg
     4861 ttaccaaaag cacaaaacca tggggaatat attttggaaa cccagctaaa agaataaaag
     4921 aaagaaaaaa agatttatta aatttagaaa tggaatatat aaaagaaaaa tcaaaaaaat
     4981 agacttaatt acaataagtt aaaaccattt tgtactttaa tgaggctatt tatacaagta
     5041 ttagttatgc catcattaac catcaatata aggccatata ttgcttagta aattcaataa
     5101 aataaaacca acaaccacta ttaaaaccta tcacactata ttagtcttat agtagaattt
     5161 agtagaattt tctataaatt aaaaaaaaaa gacttttaca tcaaaacatt aaggtgtgag
     5221 tactgattat gtggttttta tgttaagtca tcttattaga taacaagtca catactcttt
     5281 ttatccacat ctttatagat atttcagaat gatcctgata aatcagtcga tattccgata
     5341 cttatatata cctaatcaat gttagaatac ccatcaatcg cttgacccgc tggtttgaag
     5401 agtctaatct catagcggtt tggcatactg ttaaaatatg cttatataac aaaactctta
     5461 tattacagac acataagggt tttaaaagag aaattttggc gctctggggg ccgtaaaccc
     5521 agtgcggtag ctatggatac tatttaaatt atcatagtac tatgaaaaaa atattattat
     5581 tagatatttg tggaacgata acctctaaaa atacaactat agattttata aattatatag
     5641 gattcaaacc atctcatata aagcttttaa taggaaaaat catgtggaaa tttttccaca
     5701 atgatatttt aagacgatgg tatttactag aattaaaagg aattgacaaa aaagaacttc
     5761 aaacacttgc agaaaaatat atagcacagc taaaatttga caaagaaatt ctaaaatata
     5821 tcagttattt acaagaaaat ggatatgata tatatatcat atcagcaaca ctagattgta
     5881 ttgctaatga aatttcaaaa atagtcaatt ctaaaagaat ttattcatca gaactatcct
     5941 ttaaaaataa tgtttgctgt ggttctttaa aaaaagattt gttagataaa aaattaattg
     6001 aattatataa agataatata ttctataata acattacatt gactattagt gataatgaag
     6061 gtgatctaga tgttatgaaa cactctaatt attctattgc tgtagtccga aataaaaaaa
     6121 aacaatcttt ttggaagaag aaaaacattt tatctttacg taggtattaa aatgaatttt
     6181 ttttatattc ccatcattta tacatttaaa accagattaa aaaatttagt ttctttattc
     6241 tcatggttta ttatttgtcc tttattgtta tttattttat taataacaaa agagcaaggt
     6301 tctataacat tatcgataat aatatgttat attctttctt ttatacacac ctataatctt
     6361 tatgaaattg gatatattat taatgatacc gaaactataa aaaaagagtc taatccgacg
     6421 ttaagattat ctgatagcaa actaagtttt tattataaaa aaagattaag tatatattta
     6481 gaacgaatca tattatcttt atttatttca attatattaa tttattatta taacacaccc
     6541 ttaatagcca tttcatattc ttggttgtta attccatttt atttaatata taataatatt
     6601 agaaacatat ataatcttcc tattcatttt attcttgttg tcttaaggta cacatcgcca
     6661 ttaattttaa ctattaatct aaatccagaa gatttcttct atattattct agtttttcct
     6721 ttagtcaatc taattgagcg tgcttctgaa aaacgattca atataaattt cttaataaaa
     6781 agaagaaata aaattagaaa tttaagaata ttttattact ttattattct tttgattttt
     6841 ataacgttag atatattgaa ttacaacatc ccttcttata ttataatagc gagcttttat
     6901 atgttgttat atagaatttt atctccaatt ataataaata aaacaaaatg actaattaca
     6961 tagaaatata tactatattg tctttaataa tatgttcttt tttgatctta acctatgatt
     7021 tttataagtc acccaaaaat gtcctattaa atttatctag ttattctcta ctttattcta
     7081 ttttttacta tggtgtacca ttcctaaata tagaatattt atttgagtat tttgattatt
     7141 ctatttcaaa ctcaacgtta ataattacaa gattttattc aatctggacg ctatttatat
     7201 ttttagcgct atatttgtta ataagaaata acaattcaac attgatagac ctaaaaaaaa
     7261 gaaattacag cccacctaat atatttataa tcactatcat tatattccta ttgacactct
     7321 caatttatat tctttctaaa gttgggagtg atttgatagc taatttttct aatagagtaa
     7381 gtgcttatac aatatacgta caatataaag taaagtataa attacctatt ctttttatat
     7441 tccttttata ttgtatatat accttaaaag ttaatagaaa ccaaaaaata aattttatat
     7501 attatatcgt acctttaata ttcatgacac ctgaattatt atcgggtgga agaggcatgt
     7561 tatttactta tatattattt ttatttgtat tgagcatatt aaaactcaaa gataatacta
     7621 taaaaaaagc atttctattt attgcgagtt tattatttat tatggttttt ataagatatt
     7681 tactcactag gaatattaat gagaatacct ttgaatatac tatatatagt ttttttgctg
     7741 agttttatca cactgccttt acgactccat atattttaga acataatatt ttcagttata
     7801 cagattcaat aaaatatata atatatccat ttagtaaatt tttcacacca ataactaatt
     7861 atttagatat aaatactcaa cttccttggt atgcagatat tgtttcagaa catataaata
     7921 gacattttgg tttcgctggt aatattatat ctgaagcatt atattactgg ggaccaattg
     7981 ctccaataat ttatccttta ataatatcac taattattat cattcataat aattataaaa
     8041 atagtataac tggcttcttc ttttttataa taatggcaat aaatttgaga ttattcttta
     8101 gaggcagttt ttgggattct tatacatcca ttttaatatg gttttttatt ttttattttt
     8161 tattgttagc attttcttta aaaagaaaaa acgagtataa atataaaatg aaccaaataa
     8221 cgtcttcaat gttaagaata ttaaccttaa ttgctaaatt tttattctca atttatttaa
     8281 ctaaatacta taatattact gttgttggta tatacggtat tatttcttct attgtgagtc
     8341 ttttaatcta tctttctggt ttagaattat attcaatact cactcctttt tataataaaa
     8401 attcaaaatt agactcattt ttatttagta atcagatgtt tttttctatt atatctactt
     8461 tatcattagc tttttttata ttaccagtta tatattctta ttataattca tatatacaaa
     8521 tttttttatt taccattatc attattacag tcgaaatatt ccagcaagaa atatacagga
     8581 tactgctaat aagaggaaag gcattagttg ccaatatagc ttactttata aggaacgggc
     8641 tatggtgtta tgttttcatt ttatatagct atctactaaa aatagatgtg caaaatatta
     8701 caactgcatt tctttctata tgggggggag ttggattatt gggttgtata ttctcatata
     8761 ttatggtgag aaaagaaaaa atatatttca aatataacaa tattaggaaa agattcattt
     8821 ttttaaatat aaaaaaagca tttcctattt ttttatcatc tatatttatg agaataatta
     8881 taacctcaga taaaatgata ctaggattat taactaactt ataccaagtt ggtatttaca
     8941 cttttttcat tagtatcagt aacgcatttc aggcattaat tgatgctgga gctgttagtc
     9001 ataaatataa agaaataata aattctagta agaactacga ttatttaaaa ttaaaaaagc
     9061 tatttattta cattggcaaa acaagtattt gtctttatgt tatcacgtta acctcaacca
     9121 ccttattttt taattttttc aattttcatg attatctaga aaatatttta tgcttatcaa
     9181 taatactttt tagctctata tttttttcct ttggtaatat aactaaacta gttttagtat
     9241 cacaaagaaa aaagaaagaa atactttatc catctatatt atctataaat atttttttct
     9301 ttataatact atttaattgc ttgttaaaaa acagtatggt tataatgtcg gtaagcttac
     9361 tactatgctc aatagcctat gtaatattat cgtttaaata tatctatatt aataggagtt
     9421 atagtgaata atcaagtcac aatattaata aatagtttag tacaaggagg ggctgaaaaa
     9481 gtatcattgc gtatatcaga tattttcgaa gaatatggaa taacgaataa ctatgttatt
     9541 gtaagtaata atatatttta taatacaaat aaaaaaataa cctgcctatc aaataaacct
     9601 aaagtttcaa aattaaaaaa aatattttta ttaaaaagat tttttaaaga aagtaacacc
     9661 gtcctatgct tttctcttga tttagcatgt tacttaatat tattgagatg tttaggccaa
     9721 tttaaaggta aaattatctg tcgctttata aataatccta ataatgaggt tcatggtttt
     9781 acctctaaaa ttaagaaaaa aattctattt tctatcctaa aaaaatctga ttttataatt
     9841 tgtcaatcat taaatatgca gaaaatatta ttaaatgatt attctttcaa taaagaaaag
     9901 acaatcacta tttataaccc tatagaattc aataataata aaattactca aaaaaacaag
     9961 agtacaataa acctactatt tattggtcga ttaactcatc aaaaaaatct ttttgatatc
    10021 atcaaaatat cattagaatt aactaagaaa aacattaatt ttttatggaa tattgttggc
    10081 gatggtgaag aatataataa ttttaaaact ttgattaaaa aaaataactt atctaactat
    10141 atccatttat taggagcaaa aaaaaacgtt tctcaatatt atgaagaagc cgatataact
    10201 actttagttt ctcattatga aggtatgcct aatgtgctat tagagtctat agctcatggt
    10261 acgccagtaa ttagttatga ttgtattagt ggtccttcag aaattataca agaaaatata
    10321 aatggatttc ttataccaat atatgatact aaaaaattcg cagataaaat aatggatata
    10381 gaaaaaataa aaaaattaaa atcaaaagat ttatataata ccattcccaa atttcaccct
    10441 gacataatta tcaaagaata taaaaaaata ttataaggat taacaataaa tgttacctaa
    10501 atttagcgta ttaatgtcta tttataacaa ggaagaagaa aaaaatctac tcgaatgctt
    10561 ggatagttta aagaaacaat tggttaaacc cgacagaaac tataattgtt tatgatgggc
    10621 caattagaaa agaattagat gatattattg aaaaatatag tactgcatta aatattataa
    10681 aaataaaact agaaaagaat gttggattag ggcaagcatt aaatataggg ttaactcatt
    10741 gtaagaatga aattattgct aggatggata cagatgatat tgctatccct tcaagattta
    10801 aaatccaatt aagctatttt tcaaaagatc ctgatttagc tgttcttggt agtaatattg
    10861 atgaatttat aatatcaacg tccgaaacta caggtacaag aaaagtacct agcgaataca
    10921 atgatataaa aaaatatgct ttatttaaat gccctctaaa ccatatgact gttatgttta
    10981 gaaaaaaaat agttcaagat gtcggtagtt atatacatca tcattatatg gaagattata
    11041 atttatggtt aagaattctg gctagtaatc ataaaatact caatgtagaa caatctcttg
    11101 tatatgctag agttaacaaa aatactttat tacgcaggag aggtctctcg tatataaaga
    11161 gtgaatataa attagcaaaa ttaaaaaaat cactaaagct acagaaaaga gttagttcta
    11221 ttcttacttt tattcttaga gcatctcctc gcttattacc accatttatt ttaaaattga
    11281 tctataaaat taatagagat taaatatgaa aattaccatc tccggtaccg gctatgtcgg
    11341 tctatctaac gggatactcc tttcccagca caacaaagtt gttgcattag atatcaataa
    11401 agaaaaagtc gaactattaa acaaaaaaat ctctcctatc tctgataaag aaattgaaga
    11461 gtttttagct actaaagaac tcgactttac agcaacttta gataaagaat tcgcttataa
    11521 agatgctgaa tttgttatca ttgccactcc taccgattat gatcctaaaa ctaactactt
    11581 caacacctct tctgtggagt cggttattcg tgatgtttta gaatataatc caaacaccac
    11641 tatgattata aaatcaacca ttccggttgg ttttactaaa cagatgagag aaaaatatca
    11701 taccgataat attatcttct ctcctgagtt cttacgtgaa ggtcgcgccc tttacgataa
    11761 cctctaccca tcacgtattg tgattggtga gcaatctgca agagcagaga aatttgctga
    11821 gcttttacaa caaggggcat taaaaaaaga tattgatgtt ctttttactg attctacaga
    11881 agcagaagcc attaagttat ttgctaatac ttatctcgca ctgcgtgttg cttattttaa
    11941 tgaactcgat agctacgccc aagcttacaa tttaaatgca agacaaatta ttgaaggtgt
    12001 ctgtttagat ccacgtattg gtaatcacta taacaatcca tcgtttggtt atggtggcta
    12061 ctgcttacca aaagatacta aacaactatt agccaactac gaatcagtgc ctaataatat
    12121 tattggcgcc attgtggaag ctaaccgcac tcgtaaagac tttattgctg attctattat
    12181 cgctaaatca ccgaagattg ttggtgttta tcgtttaatt atgaaatcag gctctgataa
    12241 tttccgctcc tcttcaattc aaggcattat gaagcgtatt aaagcaaaag gtattgaagt
    12301 ggtaatttat gagccagaaa tgaaagaaga gactttcttt aactcaaaat taattcataa
    12361 tttagatgag tttaaagcca tgtctgatgt cattattacc aatagaatgg cttcagcact
    12421 aaacgatgtt gaagaaaaag tttatactcg agacctcttt ggtaacgatt aattaataga
    12481 aacggattaa ttacgtaatg aaatatttag tcacaggtgc tgctggattt attggttttc
    12541 atctaataaa aaaactgatc cagcagggtg aaaccgttgt tggtattgat aaccttaatg
    12601 attattatga tgttgcttta aaagaagcta gacttaatct tttaaatcaa ctagataatt
    12661 tctctttttc ttttattgat ttagctgata gagaaaaaat tgctcaatta tttgaggctg
    12721 agaaatttga tagggttatt catttagctg cacaagctgg tgttagatat tcactaatta
    12781 atccgttttc ttacgctgat agtaatttaa ctggttttct aactatttta gaaggttgcc
    12841 gacacaataa cgttaagcat cttgtttatg catcatcaag ttcagtctat ggcttaaatg
    12901 atgaattgcc attttctcct catgatcagg ctaatcatcc tgtttcttta tatgcagcca
    12961 ctaaaaaagc taatgaatta atggcgcata gctattctca tttatatggt attccgacta
    13021 ctggattacg ttttttcaca gtctatggcc cttggggacg ccccgatatg gcactattta
    13081 aatttactaa agccattatt aacaatcagc ctatagatat ttataaccat ggtgaaatga
    13141 aacgtgattt tacttatgtt gaagatattg tagaaggtgt tacacgtatt gctgatgtga
    13201 tccctacagc ccagcaagat tggaaagtaa gtacaggtac tccagcagat agttctgcac
    13261 cctataaggt ttataatatt ggtaatggat ctccggtaaa tttaatggat tacattagtg
    13321 cattagaaat acatttaggt aaaaaagcag ataaaaatat gttaccaatg caacccggtg
    13381 atgtgtacac cacatgggca gatactgagg atttatttaa agcaactggc tataaacctc
    13441 aaacaagcgt tgatgaaggt gtaaaacagt tcgttgattg gtataaaaac tattatcaag
    13501 taaaataaat aatgctcaat atcgtcttat ttgaacccga aattccaccg aatacgggta
    13561 atattatccg cctgtgtgcc aacacaggct ttcatcttca tttgatccaa ccattgggtt
    13621 ttacttggga tgataaacgg ctacgtcgtg cagggcttga ttatcgtgag tttgctgata
    13681 tcaaacagca tcacgattat tatgcttttt tagaaagtga aggtttaagc cctgataatg
    13741 cacaatcccc gacaggtgca cgattgtttg cattaaccac taaaggtact cccgcccata
    13801 gtaatgtaag ttatcaagat ggggattatc tgctatttgg ccctgagaca cgaggtttac
    13861 caccttatgt gttggataat atgccaacac aacaaaaaat ccgtgtgcca atgttagcgg
    13921 atagtcgtag tatgaatctt tctaatacgg ttgccgtggt tgtttttgag gcatggcgtc
    13981 aattaggcta tccgggtgca ttattaagag attaatttag tggttagttt gaataatgaa
    14041 agttactttc attcatacta attacttgat tttatcttta tctataagct aaaaaaggtg
    14101 ctaataatta tttagcacct ttctcataag cctttgttct gcatttatat ctgcctgata
    14161 tccgcaatca ctttcgtgat ataccgacac ctttccccca catcctttaa tactaatgca
    14221 tgggcgccaa taatcgcatt atcaccaata ttaatctcac caccaataat ggatgaatta
    14281 cagccaatat aaacatgatc tcctacttgg atattggctt ttccctcttt ctcttttacg
    14341 ccaatagtca ccccttgcat aatagtgaca tattccccca gttggcagta attagtcagt
    14401 acaatgccaa tcgggtgtgg taaagaaagg cctttaccaa tacgagtttt taacgagata
    14461 tcattacagt acttacgatt attgctagca ccgatccgaa tacctaattt atgtaaaaag
    14521 cgatggggag agaaataaaa atactgtgct acccgccacc aaaataggta acgggaacgc
    14581 tggcagaaaa atactcgatg aaatagtctt aaccaactaa agggtttttt atcaccaata
    14641 tattcagcct gtaagcaatc ttttagttca gcaagggtaa ccttagcttg agcatgcata
    14701 gttaaatacc atcaccacac tcaaagccgg gtacaacacc attaaagtgt tgatccatat
    14761 cgaatgacgg tttttcacta tcaggacgac cgacaatgcg ggcaggcacc cccgccaccg
    14821 tagtatgagc cggtacttca tgtaatacta ccgagccagc accaattttg gcaccacgac
    14881 caatctcgat attaccaagg attttagctc ctgcgccaat cattacccct tcacgcactt
    14941 tcggatgacg atcgccccca gttttacctg ttccccctaa ggtcacagat tgtaaaatgg
    15001 agacatcatt ttcaaccacc gccgtttcac caatcacaat acctgtcgca tggtcgagca
    15061 taataccgtg tccaatacgt gccgctgggt ggatatccac cccaaaggtg actgatattt
    15121 ggttttgtag ataaatggct agcgcttggc gattttgttg ccatagccaa tgggcaatac
    15181 ggtaggcttg tagtgcatga aaccctttta aatagagcag tggggtagaa tatttatcca
    15241 ccgcgggatc acgtaggcgt accgcggaga gatcacaagc agccgactca atcattgagc
    15301 tatcgtgact ataagcctct tcaacgattt cgcgtactgc aatggctggc atagtctgag
    15361 ttgccagctt attggccagc atatagctta atgcactacc taaattttcg tgcttgagaa
    15421 gtgtcgcatg aaagaaactg gctaaaattg gctcacaatc tgccaatgca cgcgcttcgc
    15481 ttttaatatg atcccagact cttgttagtt cttctagcga catactcact ctcatttttt
    15541 acacgaaagc ctaagtaaat acttaggctt tgaattttca aacaaatgga tagttaaaac
    15601 agatacccta agaatattat ttaaaataaa ctgactacag gctactgtct atctcatctt
    15661 tggttgctcg ccctaataaa gccaatgcag cctcttttac atctttatgt tgatataaaa
    15721 tttggtaaat ctgttcggtg attggcattt ctacacccac ttgttcggct aatgcgcgaa
    15781 cttctttggt gttacgataa ccttcgacta cctggccaat tttctcttgg gctgtatcaa
    15841 catctaaacc ttgacctagc atcataccaa agcgacgatt acgtgattga ttgtctgtac
    15901 aggttaaaac caaatcaccc aaaccagcca tgcccataaa agttgccgca tctgcaccta
    15961 aggctttacc taaacggctc atttcggcta gacctcgggt aatcagtgcg gtacgcgcat
    16021 tagcgccaaa tcccatacca tcagacatac ccgcaccaat agcaatcacg tttttaacag
    16081 cgccacccag ttgcacaccg ataaaatcag ggtttttata gactcggaaa cttttaccac
    16141 aatgaaatag ctgttgtaac tgctctaaaa agagattatc cgtcgaggcc actgcaatcg
    16201 cggtgggcaa acccgcggca agctctttag caaaagtagg gccagataat actgcgagcg
    16261 ggatttcatt acctaatact tcacgggcaa catcttgtaa taagcgacca gtatgtgctt
    16321 caagaccttt tgtcgcccaa acaacacgtg catcctgacg taaaaagggt ttgatttgtt
    16381 gcagtacttc accaaacaca tggcttggga tcaccacaag aatattacgg ctcgcttcaa
    16441 tcgctttttg caaagaagct tccatatata aactatcagg aaaggaaaca tcgggcagaa
    16501 aggcttgatt acagcgcgct tgttctaatg ccgcaacgtg cttgggatca tgcccccaca
    16561 gcacaacatc gtgcccatta cgcgctaagg taatcgctaa agcggtgccg tatgaaccgg
    16621 caccgataac tgtcatagaa gcgttcatag aagcgttcat taagcttctt caactcctga
    16681 gtttgcagca tcctgttgct gttgttgctg taagtagttc ataaacaacg catcaaagtt
    16741 aacaggtgct aagtttaatt gtgggaaagt accacgacct actaagttgg tgatacattc
    16801 acgtgcataa gggaatagta cgttcgggca gtatgcacct aagcaatgtg ctaattgtgt
    16861 accttcgata ccttcaacag taaagatacc tgcttgttgt actcacacag
//
