LOCUS       MH479421               16857 bp    DNA     linear   BCT 18-AUG-2018
DEFINITION  Providencia alcalifaciens serogroup O38 antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MH479421
VERSION     MH479421.1
KEYWORDS    .
SOURCE      Providencia alcalifaciens
  ORGANISM  Providencia alcalifaciens
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Du,Y., Guo,X. and Liu,B.
  TITLE     Development of a Molecular Serotyping Scheme and a Multiplexed
            Luminex-Based Array for Providencia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16857)
  AUTHORS   Du,Y., Guo,X. and Liu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2018) Teda Institute of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda street, TEDA, Tianjin,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Velvet Optimiser v. v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Providencia alcalifaciens"
                     /mol_type="genomic DNA"
                     /isolation_source="human urinary tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:126385"
                     /country="Poland"
                     /note="serogroup: O38"
     misc_feature    1..16857
                     /note="antigen biosynthesis gene cluster"
     gene            61..450
                     /gene="cpxA"
     CDS             61..450
                     /gene="cpxA"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor protein"
                     /protein_id="AXL96519.1"
                     /translation="MNKTLDVTSHPGSWPLYCNPSAIGSAFENIVRNALRYSNTHIAV
                     DFKEENNGILITVDDDGPGVSPADREHIFRPFYRTDEARDRETGGTGLGLAIVETAIS
                     QHKGWVKADDSPLGGLRLQIWLPEHGR"
     gene            1282..1905
                     /gene="neuD"
     CDS             1282..1905
                     /gene="neuD"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="AXL96520.1"
                     /translation="MTKKKLIIIGAGGFAKSIIDSLDEHQYSLSGFVDTYKIGTHQGY
                     PIIAKSIYHISNPSDYVYFIGIGDPVVRHEFLILLKEKKLELINVIDPTAILSKSVYL
                     GTGIYIGKMSIINSGTTILDGAVINTRALIEHGNTIGICTNISTNVVLNGDVNVGQKT
                     FVGSCTVVNGQINIGECSIIGSGSVVIRNIPDNVVVAGSPTRLIREI"
     gene            1907..2947
                     /gene="neuB"
     CDS             1907..2947
                     /gene="neuB"
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid synthase"
                     /protein_id="AXL96521.1"
                     /translation="MNKIFIVAEIGCNHNGDYQLAKKMVDEAYKAGVDAVKFQTFKAD
                     QLISKIAPKAEYQIKVTGSNESQLEMTRKLELPYEEFIKLEEYAKQLGLDVFSTPFDS
                     DSIDFLASRNQKKWKIPSGELLNLPYLEKIAKLEIPNKEIIISTGMATIDEIKMALNV
                     LHQNGIKAQDITILHCNTEYPTPFEDVNLNAITGFKEIFKDYKIGFSDHSPGYYAGIA
                     SVPLGITFIEKHFTLDKNFDGPDHKASVTPDELYLLCQGIRTIELALGSKEKQVTESE
                     RKNKIVARKSIVAKRNITKGEIYTIENITTKRPGNGISPMYWYDILGTKAENNFEEDQ
                     LIKHSGYLPQEV"
     gene            2951..4219
                     /gene="neuA"
     CDS             2951..4219
                     /gene="neuA"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="AXL96522.1"
                     /translation="MKLKKIAIIPARSGSKGLPNKNILMLLGKPLMAYTIEAAINSKL
                     FERVIVSTDSIEYKEIAEKYGAEVIIRDKELASDQATSFMVIDNILKKIPQYDYFVLL
                     QPTSPFRDESHIKEAIDIFESNDKHEFLVSVTESEKSTDLIKPIDSDNSLKHFNLDFS
                     NYRRQDSPYYTPNGAIFIGYNSSYVKQKHFFGPKATAYFMSKKDSIDIDDALDFELAI
                     CIQSKKEKEKTLLENIKNRINEKKELFNKEYPITLIGHSIFDNWDIEKLNEKEVNNLG
                     ISGISSEKYIELIFDKKLITSIGNTIVIFAGTNDITLNNWAPEKTWFWITNMVEKILL
                     INPTADIYILSVPPIRGRIDRNNHVIELLNEYLNNEVNKYNGKLKYIRLSSDFYDAFG
                     NLKSEFTYDGLHFTQEAYLHLEKELGDALK"
     gene            4216..5382
                     /gene="neuC"
     CDS             4216..5382
                     /gene="neuC"
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid biosynthesis protein P7"
                     /protein_id="AXL96523.1"
                     /translation="MKKILYVTGSRAEYGIMKPLLIALKNDPDIELTLVATGMHCDEK
                     FGLTYKNIKDDGFVIDKLIDIELNTSNNQKILKSMAICLDKFGKYFEDNHFDAVMILG
                     DRYEIFSVAIAAAMHNLPIIHLHGGEQTLGNYDEFIRHSITKMSKLHLASTDEYRQRI
                     IQLGEHPNSVFNIGSLGAENSLSLSLPTKNALEKKYGVLNREYLVVVFHPETLMSTPI
                     EQQVSELLSALNELSNIYDYIFIGSNSDTGSNIISSLINEFCEQKKTRYITSLLPEEY
                     LALCKYSCGLVGNSSSGIIEMPSLKIPTINIGFRQGGRIKAKSTIDCVCIKSKIVESI
                     LYSQSNEFQTIVKNSINPYSQNNSKENALKIIKGFIAKDNNSPKIFFDFENNEN"
     gene            5383..6456
                     /gene="gt1"
     CDS             5383..6456
                     /gene="gt1"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AXL96524.1"
                     /translation="MKILFILADITFVGGIERVIANLSNALSKHNIEVEILSLYKSNS
                     HCYFDIDKKIKISFLNESLHYDGKPGSLKRLLKHLTNIKKLRNYLKENSFDFIIANSF
                     PTAFQLYFSKKESNWIAYEHVHFNYYNYLVKKIRNLIYKKFDKIVVLTTKDEKKFSQI
                     FNNVITIHNPLSFESSSVSNLENHTLIAVGRLEKQKGFDILIDAFSKINNKNWRLNIF
                     GEGNEHKKLLQKINSLGASNIYLMGKSNDIKREMLYSSIFILSSRFEGFPMVLGEAME
                     CGLPCISFDCPNGPSDLIQHDHNGILVKNGCTDSLARNIEMLINNQKKINYLAENAKS
                     SVKELSICNITNQWMTMLHQVKK"
     gene            6453..7460
                     /gene="gt2"
     CDS             6453..7460
                     /gene="gt2"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AXL96525.1"
                     /translation="MKKNVFVCKTTWEHFITLLVCANIYNKEKEKSVIIIEKALENSS
                     FFKKIIQLEYFSECLQISLKQNRILKFILFKYRIKISLNHFLKKFTNNNTSINVFVDQ
                     SVISQYFIHKNLKLNLYEHGNGNYLVGAYPNFPILKKILGVTPGYGRSNMIENIYLQY
                     PDKAPHDIKDKTKKLDLKNLFNSLTLKQQQEIYNIFDIPEILSSDNNLIILTQPFSED
                     GFMSESKKIKIYHDIIIKNSNKKIYIKPHPRDRTDYKKLFKKDIIVFPSSFPIEILNF
                     KNIEFNTAITICSGSIYNFHYPINVEIIGTEDLPELIKPLGKIKQQSIPKDKYNSIQY
                     I"
     gene            7464..8519
                     /gene="wzy"
     CDS             7464..8519
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AXL96526.1"
                     /translation="MEFYILFFAILITLTLISLVYKNFDYTCYIVSTFIAIFFIGGRL
                     NVGADFIEYQHIYNYEEKINFDFLFEFFFKLFRWLGLSYPEASLSFFMLTLLFFHLAI
                     KDNSCKTLSLFFFILYSIVSLTSTIRQGLSLPFYLLAFNYILNLRKYYFYSFIGTLFH
                     VSSFIMLLIYPLKKIKISRPNFLFVILFSFLVGYFDLINVLIWFIDNTITFNNYHANK
                     ILTYSTRYNEPMSLISQLYRLGGVLFLFIFNNSLCKREDLNFCKNIYLMTFLFTLIFK
                     DNGVLINRLSFSTNVSLLYIAFNYDYISKKKITKEIILLFFITYFSVNYFKFILTDLR
                     YGIESAYLPYRNFIFDN"
     gene            8523..9725
                     /gene="wzx"
     CDS             8523..9725
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AXL96527.1"
                     /translation="MNIDKLLYFIFDIINKALPFLMIPFITRHVSLGEYGNIELFISI
                     YVFFSFIISFAFEGWLSSNYFKTIKLSIYIKSYLLFVLYLSLLIFLFIFIYDSFLSLT
                     IFIAFTNAILFLVTTLLRLEGKFKYAGAILLFNSVINVITLYLFFILFEPTFQSRIYA
                     LVLTYFITLSILIYIIRIKYNHILLANAELAAFKVIFLFCLPLCISMISSWIKGNIDK
                     FYIGSLLSLNDLAIYSLGFQISSVLGVITITINKIIQPSFFYKMSNDLNTKGSIFLVI
                     LTIIIGSIFYLFSIDYLFSFIFDDRYLPSMKLLPFFILSFTSSAIVMILNNYLMFHQK
                     TKLIMYQVIISSFFHCIISFYLIHYFSIIGATIAQLISSILSLLITLFFLRKLKYAKL
                     NINCNASL"
     gene            9688..10431
                     /gene="gt3"
     CDS             9688..10431
                     /gene="gt3"
                     /note="GT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AXL96528.1"
                     /translation="MPSLISIVMPAYNAEKTILESIESVISQTYLNWELIIIDDCSND
                     MTLEIIKKYSSQDSRIKLIKNEFNLGISSSRNISIKNCTGDFICFLDSDDIWIPNKLE
                     KQILLLNSGWDVVCSNYTTFSDDSNIILQHRKSPSIIKYTDMLKSNFIGNLTGIYNRE
                     KLGTFFQKKLGHEDYVMWLNILNKSKKAYCIQEPLAKYRISKKSISANKIKTMQWQWF
                     IYRNELQFSLIKSMYYFSHYIVNALKKRR"
     gene            10435..11382
                     /gene="gne"
     CDS             10435..11382
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="AXL96529.1"
                     /translation="MLTIIGGSGFIGTRLSNQLKSQNIDFKIVDIEKSEAHPEKWEFG
                     DVTKPETLIEPLKGSDVIINLSAKHKDNVHPISLYYEVNVDGAKNVCDVAEQLGIKHI
                     VFTSSVAVYGFVEKETGEDGEYHPFNHYGKSKLEAEYVYDAWQAKSADNILVTIRPTV
                     VFGENNRGNVYNLFRQIASGRFLMIGSGNNQKSMAYVENIAAFITFATTFTEGKHVFN
                     YIDKPDFTMNELTTVICGALNKPKSNIRIPYPVGILGGYCFDVLSKLTGKEFPVSSIR
                     VKKFCARTQFKSNYIGNTSFVPPVTLEQGIANTVQYEFK"
     gene            11433..12458
                     /gene="galE"
     CDS             11433..12458
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AXL96530.1"
                     /translation="MSSSCVLVTGGLGYIGSHTCVQMIQQGIQPIILDNLHNANLEVL
                     NRIETITGTKPTFYQGDVRDKAILGTIFAQHSISSVIHFAGLKAVGESVEKPIEYYDV
                     NVNGTLVLVQSMRQAGVKSLIFSSSATVYGEPEHTPLTEESAVGNTNSPYGTSKYMVE
                     RILTDLSISDNEWSISLLRYFNPVGAHNSGLMGEDPNGIPNNLTPFIAQVAVGRRKEL
                     AVFGGDYATKDGTGVRDYIHVMDLADGHIAALNYMNKDSGLYIYNLGTGTGTSVLEVI
                     AAFEKAVGKPIPYKIAERRAGDIAEYWSTPAKAERELGWKAKYTIQDMADDVWRWQSM
                     NPNGYQS"
     gene            12801..13931
                     /gene="wza"
     CDS             12801..13931
                     /gene="wza"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide export protein"
                     /protein_id="AXL96531.1"
                     /translation="MAITMSILISGCHLVPGTYISTSGKRTIDTGKQDINQLVDIYPI
                     SPKLLSEMQIAPVIAKSNPQLEEQLASYDYRVGVGDVLAITVWNHPELTIPAGSYRSA
                     ADSGNWVHADGTIYYPYIGKVSVLGKTISEIRKLVSTRLAAYLEAPQIDVSIAAFRSQ
                     KIYVSGEVTKPGSLPITNVPLTILEAFNTAGGLTEKADWDNVILTRGGKEIKISLQSL
                     VQYGDLTQNYLMLSGDVLYVPRNDAQKVFVMGEVGKPSTLTIDRAGMSITEALSKANG
                     LSQTTASATGIFVIRSTHKPAPITDSEDENPLSKMAEIYQLDLSDATSLVMGTEFKLQ
                     PYDLVYVTAAPVVRWNRLITQLLPTIASYNQLAEGTKRIYAW"
     gene            13948..14382
                     /gene="wzb"
     CDS             13948..14382
                     /gene="wzb"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phosphatase"
                     /protein_id="AXL96532.1"
                     /translation="MLMFNNILIVCMGNICRSPTAERLMQELFPQKRIHSAGLIALEN
                     QPADAQARHIAKNHHISLEENRSRRLTGHLCQMADLILVMENTHTQKLYRQFPQTRGK
                     VMLFGEWLNKTEIPDPYKHSEEMFVHVYQLMEKAAESWQGKI"
     gene            14415..16496
                     /gene="wzc"
     CDS             14415..16496
                     /gene="wzc"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="AXL96533.1"
                     /translation="MNNTEKKYSPNTDEIDLLAIIKTLKSNALTIAAFTIAAILSAGV
                     YCWLASPIYQANATLQYDKVNQASLLEKMNDGMLFGGNNGQIDSEIEVIKSRMVLGKT
                     VSDLNLDIQILPSKIFSKFLSDAAIVHIAEYQLPPDLIGQPATLTFVDDRQYTLELAG
                     QTYHGQIGIPLKQCEIHLLIDSCTAQGGEKLTLIKNDRYSAIENLRRRLSMNEASKGS
                     GIINLTIKGTNQRENIEILDNVIQNYINQNSEHRKLATNNTLIFLDNYIPKIKNKLDH
                     YENQLNTFRKQNESIDLTLEAKSALESALHVEERLNELTFKEVELQQRYTRSHPAYQA
                     LLDTRQKLLLEKEKASKNIQQLPNTQQQIIRLTRDVESEQAIYNQLVTKQRELSVLNS
                     GITADVRIIDSAESYPMAIAPKKSLIMVLASLLGLVMGFAFVIGKELLNNKIKNTEEL
                     EALGLRVYATVPFSLDEKKSRYRESKKPLSIENPADMAIESIRSLRTSVYFSVMNQDN
                     NIVMVTSASPNVGKSFITANMAVVLANAGKKILLIDTDLRKGHIHKTFGLENKSGLSE
                     FLSQHGGDSISKHQQVIENLDIISRGKSTTHSSELLMSEQFKHLLDSVKHQYDMVLLD
                     TAPILAITDPAIIGKYAGTSLLVAYYSVNTVKEIERALKHFKQNNIEITGTILNGIDE
                     KSDDYHYVYQY"
BASE COUNT     5814 a   2923 c   2745 g   5375 t
ORIGIN
        1 tcaaagcccg atgagttatg gggagatatt ctggatgatg caagcttcga agcggagcaa
       61 atgaacaaaa cgctggatgt cacgtcgcac ccaggctcat ggccgttata ttgcaatcca
      121 tccgccattg gcagtgcgtt tgaaaacata gtgcgtaacg cactacgtta ctcaaacacc
      181 catattgcgg ttgatttcaa agaagaaaat aacggtattt tgattactgt cgacgatgat
      241 ggcccgggtg tcagccctgc tgaccgcgag catatcttcc gaccgttcta tcgcaccgat
      301 gaagctcgcg acagagaaac cggtggtaca gggcttggct tagcaattgt ggaaacagcg
      361 atttcccaac ataaaggctg ggtaaaagct gacgacagcc cgctaggtgg attacgccta
      421 cagatttggc tgccagaaca cggacgctaa tcttgcatta tgaaggtctt tggtgctcat
      481 gtgccaaaga cctttttata tatcagccat atattcctat cctatctcgc ctaatattcc
      541 catccaaact caacctgact gataattaac ttattcatat tcacaaatag taatgaaatt
      601 agtgtctcaa acatttagcg atatttgcat atacggattt actacatata cttgtaagta
      661 agaaatagct cagacaattc ttataaatat tatatttcat tgattatatc gttattcgag
      721 taattacctt ttcctacacc acacgggcgt gatctattta tatattaata acatacatca
      781 agttacagct gtgattatca ttatttttta tactatctag ccgtatacat gctttttctt
      841 gactatcaga ttagccccta agtatagata ccactcaaac caatatgcca cccaatgtga
      901 aattaacaat tttttataga actatatact aataactcac ttaatggttt caaaaaggtg
      961 atcaggagat tactggcaaa gtagcaattt ttaggcgcct cacaccgata gtcactccga
     1021 tactttagtg ctattattaa gggcttttga ttgattgtgc ttacggcacg agtcaaccaa
     1081 taaaggaagg ctatcataag cagcgtttat taattgagca attatgcaaa aagtgcagta
     1141 ttttcaatta ataaacacta aattttgaaa ttcaatggca ctctgggacc cgataaccca
     1201 gtgcggtagc tatgggatct aataactact attatgtagt taaacatgat ttcttacgtc
     1261 ttaaaataga atcagaaaat catgacaaaa aagaaattaa ttatcattgg tgctggtggt
     1321 tttgctaaat ctattattga tagcttagat gaacaccaat actcattatc aggatttgta
     1381 gatacctaca aaataggaac tcaccaaggc tatccaatta tagctaaaag tatctatcac
     1441 attagcaatc cgtcagatta tgtatacttt attggcattg gagatcctgt tgttcgacat
     1501 gaatttttaa ttcttttaaa agaaaaaaaa ctagaactta ttaatgttat agatcccaca
     1561 gcaatattat ctaagagtgt gtatttaggt acagggattt atataggaaa aatgagcatc
     1621 attaatagtg gaacaacaat attagatggt gccgtaatta atactagagc tttaattgaa
     1681 catggcaata caattggaat atgcactaac atttcaacaa atgttgtatt gaatggtgat
     1741 gtcaatgttg gacagaagac ttttgtcgga agttgtactg tagtaaatgg gcagatcaat
     1801 ataggcgagt gttccattat tggctccggt tctgtagtta ttcgcaatat cccagataac
     1861 gttgttgttg caggttcacc cacaagacta attagagaaa tatagaatga ataaaatttt
     1921 tattgtcgct gaaataggtt gtaaccataa cggtgattat caattagcaa aaaaaatggt
     1981 cgatgaggca tataaagcgg gtgttgatgc agtaaaattt caaacattta aagcagatca
     2041 attaatatct aagattgcac caaaggctga ataccagata aaagtaactg gctctaatga
     2101 atcacagtta gagatgacta gaaaattaga actaccatat gaggaattca taaaacttga
     2161 agaatatgcc aagcaactag gtttagatgt cttctcaact ccatttgact ctgactccat
     2221 agattttctt gcatcacgaa atcaaaaaaa atggaaaatt ccgtccggtg agctacttaa
     2281 tttaccttat ttggaaaaaa ttgcgaaatt agaaattcct aataaagaaa ttatcatttc
     2341 tacaggaatg gctactatag atgaaataaa aatggctctt aatgtgctcc atcagaatgg
     2401 aataaaagca caagatatta ctatattaca ttgcaatacc gaatatccaa caccttttga
     2461 agatgtaaac ttaaatgcta ttactggatt taaagaaata tttaaagact ataaaatagg
     2521 cttttctgac cattctcctg gatattatgc aggaatagca tcagtacctc ttggtattac
     2581 ttttattgag aaacatttta ctctagataa aaattttgac ggcccagacc ataaagcctc
     2641 agttacccct gatgagctat atttattatg ccaaggcatt cgtactatag agttagcttt
     2701 aggaagcaaa gaaaagcagg taactgagtc agagagaaaa aataaaattg ttgcacgaaa
     2761 atcaatagtt gccaaaagaa atattacaaa aggtgagatc tacactattg aaaatataac
     2821 cacaaagcgc cctggaaatg gtatcagccc tatgtactgg tacgatatct taggtacaaa
     2881 agctgaaaat aattttgaag aagatcaatt aattaaacat tcaggctatt tacctcaaga
     2941 ggtgtagttt atgaaattaa aaaagatagc cattattcca gctcgttctg gttcaaaagg
     3001 attaccaaat aaaaatattt tgatgttact aggtaaacca ttaatggctt atacaatcga
     3061 agccgctatt aattcaaaac tattcgaacg agttatcgta tcaactgact ccatagaata
     3121 taaagaaatt gcagagaaat atggtgctga agtcattatt cgagataagg aattagcatc
     3181 agatcaagct acctcattca tggttattga taatatatta aaaaaaatac ctcagtatga
     3241 ttactttgta ttattacaac cgacatcgcc atttcgtgat gaatctcata ttaaagaggc
     3301 tatagatatt tttgaatcca atgataaaca tgagtttcta gtttcagtaa ctgaaagtga
     3361 aaaaagtaca gacttaatta aaccaattga ttcagataac tcgttaaaac attttaacct
     3421 agactttagt aattatcgaa gacaagactc accatattat acccctaatg gggcgatatt
     3481 tattggatat aacagttctt acgtaaaaca aaaacatttc tttggtccta aggccacagc
     3541 atactttatg tctaaaaaag actctattga tattgatgat gctttagact ttgaacttgc
     3601 catttgtatt cagtcaaaaa aagaaaaaga aaaaacctta cttgaaaata taaagaatag
     3661 aattaatgaa aaaaaagagc tatttaataa agaatatcca attactttaa tagggcactc
     3721 tatatttgac aattgggata ttgaaaaatt aaatgaaaaa gaagttaata atttagggat
     3781 ttctggaatt agctcagaaa aatacattga attaatattc gataaaaaat taattacaag
     3841 catcggcaat actattgtta tttttgcagg aacaaatgac attaccctaa acaattgggc
     3901 acccgaaaaa acgtggttct ggattactaa catggtagaa aagatattat taattaatcc
     3961 aactgctgac atctatatat tgtctgttcc accaattaga ggccgtatag atagaaataa
     4021 tcatgtcatt gagttgctaa atgagtatct aaataatgaa gtaaataaat ataatggcaa
     4081 actcaaatac ataaggttaa gtagtgattt ttatgatgcc tttggaaatc taaaatctga
     4141 gtttacctat gatggtttgc atttcactca agaagcttat ttgcacttag aaaaagaatt
     4201 aggtgatgct ttaaaatgaa aaagatatta tatgttaccg gctcaagagc tgaatatggc
     4261 ataatgaaac cgctattaat tgcattaaaa aatgaccctg atattgaact gacattagta
     4321 gctactggta tgcactgcga tgagaaattt ggtttaactt ataagaatat aaaggatgat
     4381 ggttttgtaa ttgataaatt aattgatata gagctaaata catctaacaa tcaaaaaata
     4441 ctaaaatcaa tggctatatg tttagataaa tttgggaagt attttgaaga taaccatttt
     4501 gatgctgtta tgattttagg tgatagatat gaaatctttt ctgttgctat tgctgctgca
     4561 atgcataatt taccgattat tcacctgcat ggaggagaac aaacattagg taactatgac
     4621 gaattcataa gacattcgat tactaaaatg tctaaactac atttagcatc gaccgatgaa
     4681 tatcggcagc gcatcatcca attaggtgaa caccctaatt ccgtatttaa cataggctct
     4741 ttaggcgcag aaaatagttt atcattatct ttgccaacta aaaacgcgtt agaaaaaaaa
     4801 tatggtgttc ttaatagaga gtatcttgta gttgttttcc atccagaaac tctaatgagt
     4861 acccctatag aacaacaagt atctgaactt ctcagcgctc ttaatgaact ttcaaatatt
     4921 tatgactata tcttcattgg ttcaaattca gatactggat caaatataat ttcatcttta
     4981 attaacgaat tttgcgaaca aaaaaaaact cgttatatta catcattatt acctgaagaa
     5041 tacttagctt tatgtaaata ttcatgtgga ttagtcggaa attcctcatc tggcataata
     5101 gaaatgccat cattaaaaat accaacaata aatattggtt ttcgtcaagg cggtagaatt
     5161 aaagcaaaaa gcacaattga ctgtgtatgt ataaaatcca aaatagttga gtctattctt
     5221 tatagccaat ctaatgaatt ccaaactatt gttaaaaact caattaatcc atactcacaa
     5281 aataattcaa aagaaaacgc attaaaaatt atcaaaggat ttatagccaa agataataat
     5341 agccctaaaa ttttttttga ttttgaaaat aatgagaatt aaatgaaaat attatttatc
     5401 cttgccgata tcacttttgt tggcggaata gaacgagtta tagcaaacct atcaaacgct
     5461 ctatctaaac ataatataga agtagaaata ctaagtttat acaaatcaaa tagccattgt
     5521 tacttcgata tagataaaaa aataaaaatt tcatttttga atgaaagctt gcactatgat
     5581 ggaaaaccag ggtcactaaa aaggctatta aagcatttaa caaatataaa aaaattgaga
     5641 aattatctaa aagaaaactc ttttgatttt ataatagcaa attcatttcc tactgccttt
     5701 caattatact tttcaaaaaa agaaagtaat tggatagcat acgaacatgt tcattttaat
     5761 tactataatt atttagtaaa aaaaatcaga aatttaattt ataaaaaatt tgataaaata
     5821 gttgtgttaa caactaaaga tgaaaaaaag tttagccaaa ttttcaataa tgtaataacc
     5881 atccataatc cattaagttt cgagagctca tcggtctcta atttagaaaa tcatacactg
     5941 attgcagttg gacgtttaga gaagcaaaaa ggtttcgaca tactaattga tgcattctca
     6001 aaaataaata acaaaaattg gcgattaaat atttttggcg aaggcaatga acataagaaa
     6061 ttattacaaa aaataaattc attaggagcc tctaatattt atttgatggg aaaatctaat
     6121 gatataaaaa gagaaatgct atactctagt attttcattc tttcatccag atttgaaggt
     6181 tttcctatgg tattgggtga agcaatggaa tgtggcttac catgtatatc atttgattgt
     6241 ccaaatggac catcagattt aattcagcat gaccataatg gaattctagt taagaatggt
     6301 tgtactgatt ccttagcaag gaatatagaa atgctaataa acaatcaaaa aaaaataaat
     6361 tatttagctg aaaatgctaa atcttctgtt aaagaactat caatatgcaa tataacaaat
     6421 caatggatga caatgcttca tcaggtgaaa aaatgaaaaa aaatgttttt gtatgtaaga
     6481 ccacgtggga gcattttatt acattattag tctgtgccaa tatctataat aaagaaaaag
     6541 aaaagtctgt cataatcatt gaaaaagcgt tagaaaatag ttcttttttc aaaaaaataa
     6601 ttcaactaga atattttagt gaatgtcttc aaatatcatt aaaacaaaat agaatattga
     6661 aatttattct ctttaaatat agaattaaaa tatcacttaa ccattttttg aaaaaattta
     6721 caaataataa tacctcaatc aatgtttttg tagaccaaag tgttatctct caatatttta
     6781 tacataaaaa tctcaaacta aatctttatg agcacggaaa tggaaactat cttgttggcg
     6841 cttatccgaa ctttcctatc ttaaaaaaga ttcttggtgt aactcctgga tatggaagaa
     6901 gcaatatgat tgaaaatatt tatctgcaat atccagataa agcccctcat gatataaaag
     6961 ataagactaa aaaattagat ttaaaaaacc tttttaattc actaacactt aaacaacagc
     7021 aagagatata taatattttt gacatacctg aaatattaag cagtgataat aatttaataa
     7081 tattaacaca accctttagt gaagatggat tcatgtcaga aagtaaaaaa ataaaaatct
     7141 atcatgatat catcattaaa aattcaaata aaaaaatata tataaagcca catcctagag
     7201 ataggacaga ctataaaaaa ctatttaaaa aagacataat tgtcttccca tcatcttttc
     7261 ctattgaaat attaaacttc aaaaatatag aatttaatac agctattaca atatgttcag
     7321 gatctatcta caactttcat tatcctatta atgtggaaat tattggtact gaagatttac
     7381 ctgaattaat taagccttta ggaaaaataa agcaacagtc tatacctaaa gataaatata
     7441 attcaattca gtacatttaa cttatggaat tttatatatt attttttgct atactaataa
     7501 cacttactct aatatcactt gtttataaaa actttgatta tacttgttat atagtctcaa
     7561 catttattgc tatatttttc ataggtggac gtttaaatgt aggtgctgat ttcattgaat
     7621 atcagcacat atataactat gaagaaaaaa taaattttga ttttttattt gaatttttct
     7681 ttaaattatt tagatggcta ggtttaagtt atccagaagc gtcactatct ttttttatgc
     7741 ttactttatt atttttccat ttagctataa aggataactc ttgtaaaaca ctttctcttt
     7801 ttttctttat cttatattct atagtttcat taacatcaac aattaggcaa ggtttatctc
     7861 ttccttttta cctactagct ttcaactata tattaaactt gcgaaaatac tatttttact
     7921 cttttattgg caccttattt catgtatcaa gttttatcat gcttttaatt tacccactaa
     7981 aaaaaataaa aatcagtaga cctaactttt tatttgttat tctattttcg tttttagtag
     8041 gctattttga tttaattaac gtacttattt ggtttattga taatactata acctttaata
     8101 attatcatgc taataaaata ctaacttatt ctacacgcta taatgaacca atgtctctga
     8161 tttcacagtt atataggtta ggtggtgtgc tttttctatt tatttttaac aatagcctat
     8221 gtaagagaga agacttaaat ttttgtaaaa atatatactt gatgacattt ctattcacat
     8281 tgatatttaa agataatgga gtccttatta atcgactatc attttccaca aatgtatcat
     8341 tattatatat agcctttaat tatgactata taagtaagaa gaaaataacc aaagagataa
     8401 tattattatt tttcattact tatttttccg taaattactt taagtttatc cttacagact
     8461 tacgatatgg aattgaaagt gcgtatttgc cttatagaaa ttttattttt gacaactaga
     8521 aaatgaatat agacaagtta ctctatttta tatttgatat tataaataaa gctctccctt
     8581 tcctcatgat accatttatt actcgtcatg tatcattagg tgaatatggt aacattgagt
     8641 tatttatttc tatctatgtt ttttttagct ttattattag ttttgcgttt gaaggatggc
     8701 tatcatctaa ttattttaaa accataaaat tatctatata cataaagtca tatttattat
     8761 ttgttctata tttatcttta cttatctttc ttttcatatt tatttatgac tcgtttttat
     8821 ctttaactat ctttatcgcc tttactaatg ctattctttt tcttgttact actttactta
     8881 gattagaagg taaattcaaa tatgcgggag caatactgct cttcaatagt gtaattaatg
     8941 taattacgtt atatttattt tttatactct ttgagccaac atttcagagt cgaatttatg
     9001 cccttgtatt aacatacttc attacactat ctattcttat ttacattata agaataaaat
     9061 ataaccatat acttttagct aatgcagaat tagcagcatt taaagtgata tttttatttt
     9121 gtcttccttt atgcatctca atgatcagct catggattaa aggaaatatt gataagtttt
     9181 atattgggag tttactatca ttaaatgatc tagccatcta ttccttgggg tttcaaatta
     9241 gctctgtttt aggtgtaata accattacta taaataaaat aattcaacct tctttttttt
     9301 ataaaatgag caatgattta aatactaaag gaagtatttt tttagtgatc ttaacaataa
     9361 ttatcggctc tattttttat ctgttctcta tagattactt attctcattt atctttgatg
     9421 atagatattt accttctatg aaactactcc cattttttat cttatcattt acttcatcag
     9481 ctatagttat gatattaaat aattacttaa tgtttcatca aaaaacaaaa ttaatcatgt
     9541 atcaagtaat aatatcttca ttttttcact gtataatcag tttttatctc attcattact
     9601 tttcaattat tggagcaaca atagcacagc taatatcgtc tatcctatca ttgttaatta
     9661 cattattttt cctgagaaaa ttaaagtatg ccaagcttaa tatcaattgt aatgccagcc
     9721 tataatgcag aaaaaacaat tttagagtca attgagagcg ttatatctca aacatattta
     9781 aattgggagt taataattat tgatgattgc tctaatgata tgacgcttga gattataaaa
     9841 aaatattcaa gtcaagatag tcgaatcaag cttattaaga atgaatttaa tcttggaatt
     9901 tcttcttcaa gaaatatatc aatcaaaaat tgcacaggtg attttatttg ctttctagat
     9961 agtgatgaca tttggattcc taataagcta gaaaaacaaa tacttctact aaatagtggg
    10021 tgggatgttg tatgttccaa ttacactaca ttttccgatg atagtaatat catactacaa
    10081 cacaggaaat ctccatcaat aattaaatat actgacatgc taaaatctaa ctttattggt
    10141 aacctaacag gcatatataa cagagaaaag ttaggtacat tttttcaaaa gaagcttggc
    10201 catgaagatt atgtaatgtg gctgaatata ttaaataagt caaaaaaagc atattgtatt
    10261 caagagcctc ttgcaaaata tagaatatct aaaaaatcta tatctgcgaa taaaataaaa
    10321 accatgcaat ggcaatggtt tatttatcgt aatgagctac aattttcact aataaaatcc
    10381 atgtattact tttctcatta catagtcaat gccttaaaaa agagaagata atttatgcta
    10441 actatcatcg gtggttccgg ttttatcgga acacgtctgt caaaccaatt aaaatctcaa
    10501 aatatcgatt tcaaaattgt tgatattgaa aaaagtgaag cccatcctga aaaatgggaa
    10561 tttggtgatg tcaccaagcc tgaaacactt attgaaccac tgaaaggtag tgacgttatt
    10621 attaacctat cggcaaaaca caaagataac gttcacccta ttagccttta ctatgaagtg
    10681 aacgttgatg gtgccaaaaa cgtctgtgat gtcgctgaac aattggggat caaacatatt
    10741 gttttcactt catcggttgc tgtttatggc ttcgttgaaa aagaaaccgg ggaagatggc
    10801 gaatatcatc catttaacca ctatggtaaa tctaagttag aagctgaata cgtttacgat
    10861 gcatggcagg caaaatctgc ggataacatc ttagtcacta ttcgcccgac cgttgtattt
    10921 ggtgaaaata accgtggcaa cgtttataac ctcttccgcc aaattgcgtc tggtcgattc
    10981 ttaatgattg gttctggtaa taaccaaaaa tcaatggctt atgtggaaaa tatcgcggcg
    11041 tttattacat tcgcaaccac cttcactgaa ggaaagcatg tattcaatta catcgataag
    11101 cctgatttta caatgaacga attaacaacg gtcatttgtg gtgcccttaa taaaccgaaa
    11161 tctaatatcc gtattccata tccagttgga attttaggtg gctactgttt tgatgtgctt
    11221 tctaaactaa cgggcaaaga attcccagtg agtagcattc gagtgaaaaa gttctgtgct
    11281 cgtacacaat ttaaatccaa ttacattgga aatacctctt ttgttccacc agttactttg
    11341 gaacaaggta tcgccaatac tgtgcaatat gagtttaaat aaaacccatt tttcataatc
    11401 tcaattttgg aatatataga caggacacaa ccatgagcag ttcttgcgta ttagtcaccg
    11461 gcggtcttgg ctatatcggc agccatacat gcgtacaaat gatccaacaa ggtattcagc
    11521 cgattatttt agataatctt cataatgcca accttgaagt gcttaatcgt attgaaacca
    11581 tcacgggaac taaaccgacg ttttatcaag gtgatgttcg tgataaagcc attttgggca
    11641 ccatttttgc tcagcattca attagctcag tgatccactt tgcggggtta aaagcagtcg
    11701 gtgaatctgt tgaaaagccg attgaatact atgatgtgaa tgtaaatggt accttagttt
    11761 tagtgcaaag catgcgtcag gcgggtgtta aaagcttaat ttttagctcc tctgcgactg
    11821 tgtatggtga gcctgaacat actccgctaa ctgaagaatc cgctgtcgga aatactaata
    11881 gtccatatgg cacgagcaaa tatatggttg agcgtatttt aactgacctc agtatctcag
    11941 ataacgaatg gtcaatttcc ttactacgct acttcaaccc agttggtgca cataattctg
    12001 gcttgatggg tgaagatcct aatgggatcc caaataacct gaccccattc attgcgcaag
    12061 tagctgtcgg tcgccgtaaa gaacttgctg tattcggcgg ggattatgcc accaaagacg
    12121 gcactggcgt tcgcgattat atccatgtga tggatttagc tgatggacat attgctgcgt
    12181 taaactatat gaataaagat tcagggcttt atatctataa tctgggtacc ggtacaggaa
    12241 ccagcgtgct ggaagtgatc gctgcttttg aaaaagctgt cggtaaacca atcccttata
    12301 aaattgccga acgtcgtgca ggagatattg ctgaatattg gtcaactcca gcaaaagcag
    12361 agcgcgagct tggttggaaa gccaaatata ccattcagga tatggctgat gatgtatggc
    12421 gctggcaatc tatgaatcct aacggttatc aaagctaaat cattctttat atagcatcaa
    12481 ttaagcgagc caccggctcg ctttttttat tttttacatt ctcaaacttt attctcacca
    12541 cacagttaac ctactcactc cctagttcgc ttctagtcta ttcctccaac cacctttcac
    12601 tcacaaaaca ttttctgcat caatatcatt ttttgtttta ttatattttt atataacatc
    12661 gatttttgga tgaattattt ggttttttgt ttaaaactga actattatcc cgagctgact
    12721 ttgtgtcgtg taacttcgat tgctaaaaat taccattatc catcctgatt agggctatta
    12781 tcatgttcag taaatttctc atggctatca caatgagcat cctaatttct gggtgtcatc
    12841 tcgtcccggg aacctatatt tccacatcag gaaaaaggac tattgatact ggtaagcaag
    12901 atatcaacca acttgtggat atttacccta tctcccctaa gctgctaagc gaaatgcaaa
    12961 ttgcccctgt gattgccaaa tctaatcctc agcttgaaga gcagctagca agttatgact
    13021 atcgtgttgg cgtgggggat gtgctggcaa ttaccgtatg gaatcaccct gagctcacta
    13081 tccccgctgg ctcctatcgc agcgctgcgg attcagggaa ttgggttcac gcagatggca
    13141 ccatttatta tccctatatt ggaaaagtct ccgttcttgg taaaacaatc agtgaaatca
    13201 ggaaattagt cagcacccga ctagcagctt accttgaagc gccacaaatc gatgtcagca
    13261 ttgccgcctt tcgctcacaa aaaatctatg tgtctggcga agttaccaaa ccaggttcct
    13321 tgcccatcac caatgtccct ctcactattt tggaagcctt taataccgca ggcgggttaa
    13381 ccgaaaaggc ggactgggac aatgtaatcc tcacccgtgg gggaaaagag ataaaaatct
    13441 cgctgcaatc gttagtccaa tacggtgatt taacccaaaa ctacttaatg ctatctggcg
    13501 atgttctcta tgttccccgt aatgatgcgc aaaaggtctt tgtgatggga gaagtgggga
    13561 aaccatcaac attgactatc gaccgtgcag gaatgagcat cactgaagcg ctaagtaaag
    13621 ccaacgggct cagccaaact accgcaagtg cgacagggat ttttgtcatt cggtcaacgc
    13681 ataaacccgc accaataaca gattctgaag acgagaaccc cctttctaaa atggcagaaa
    13741 tttatcagct cgacctctcc gatgcgacgt cactcgtgat gggaaccgaa tttaaattac
    13801 agccttatga ccttgtttat gtgacagcag cacctgttgt tcggtggaac cgtttaatta
    13861 cccaattact gccaaccatc gcctcataca accagcttgc cgaagggact aagcgtatct
    13921 atgcatggta atcttatttt taatcaaatg cttatgttca ataatattct tatcgtgtgt
    13981 atggggaaca tctgccggtc acccactgca gagcgtttaa tgcaggagct tttcccccaa
    14041 aaaaggatcc actccgcagg tctgattgca ctggaaaatc aacctgctga tgctcaagct
    14101 cgccatatag caaaaaacca tcacatttct ttggaggaga atcgttctcg tcgtttaacc
    14161 gggcatttat gccagatggc ggatttgatc ctcgtaatgg aaaacactca tacccaaaaa
    14221 ctttatcggc aatttccgca aacccgagga aaagtcatgc tatttggtga atggttgaat
    14281 aaaaccgaaa tcccagatcc ttataaacat agcgaggaga tgtttgtgca tgtttatcag
    14341 cttatggaaa aagcagccga gtcgtggcaa ggaaagattt agactctatt taaacttatt
    14401 taaaaactaa ctctatgaac aatacagaaa aaaaatattc accgaacacc gatgaaatcg
    14461 acttactcgc cataataaaa acactgaaat ctaacgcgct aacgatagct gcgtttacta
    14521 ttgccgccat tttaagtgcc ggagtgtatt gctggctcgc ttctcctatt taccaagcga
    14581 atgctacatt gcagtacgat aaagtaaacc aagcatcact attagaaaaa atgaatgatg
    14641 gaatgctttt tggcggtaat aacgggcaaa tcgattcaga aattgaagtc attaaatcca
    14701 gaatggtgtt aggcaaaacg gtgtcagatt tgaatctaga tattcagatt ttgccttcaa
    14761 agatattctc caaatttctg agtgatgcgg ctattgttca tattgcagag tatcaactcc
    14821 ccccagattt aatcggtcaa cccgccactt taacctttgt agatgatcgc caatatacgc
    14881 tagaacttgc aggccaaact tatcatgggc aaattggcat ccctttaaag caatgtgaaa
    14941 ttcatttatt gattgattca tgtaccgctc aaggtgggga aaaactgact ttaataaaaa
    15001 atgatcgtta ttctgctatc gaaaatttac gacgccgtct cagtatgaat gaagctagta
    15061 aaggctctgg tatcattaac ttaacaatta aagggacaaa tcaacgagaa aatatcgaga
    15121 ttttggataa tgttattcaa aattatatta atcaaaatag cgaacatagg aagctagcta
    15181 caaacaacac gttgattttt ctagataact acataccaaa aataaaaaat aagctcgacc
    15241 attatgaaaa tcaattaaat acgtttcgta aacaaaatga gtccattgac ctaacgctag
    15301 aggccaaatc cgcgcttgag agtgcattac atgtagaaga aaggctaaat gagcttactt
    15361 ttaaagaagt ggagcttcaa caacgatata cccgcagcca cccggcttat caagccttac
    15421 ttgatacacg ccaaaagttg ctactcgaga aagaaaaagc cagcaaaaat attcaacagc
    15481 tgccaaacac ccagcagcaa attattcgtt taactcgtga tgttgaatcc gagcaagcta
    15541 tttataatca gttggtgacc aagcaacgcg aactcagtgt gctgaattca gggatcactg
    15601 ccgatgtgcg aattattgac tctgcggagt cttaccccat ggccatcgct cccaagaaaa
    15661 gcttaatcat ggttttggcc tctttattgg gtctcgtgat gggcttcgcc tttgtgatcg
    15721 gaaaagagct tctcaataat aaaattaaga acacggaaga actcgaggcg ctaggccttc
    15781 gtgtttatgc caccgttcca ttctcgttgg atgaaaaaaa atcacggtat cgcgaaagta
    15841 aaaagcccct atccattgaa aatccagcag atatggcaat agaatccatt cgttcattaa
    15901 gaacaagtgt ctatttttct gtgatgaatc aagacaataa tattgtgatg gtgacaagtg
    15961 catccccaaa tgtaggaaaa agttttatta ctgccaatat ggctgtcgtc ctcgctaatg
    16021 caggtaaaaa aattctgctt attgataccg atttacgcaa aggacatatt cataaaacat
    16081 ttgggttaga aaataaatct gggctttcag aattcttatc tcaacacggc ggggattcca
    16141 tctcaaaaca tcaacaagtt attgagaatt tggatattat cagtcgagga aaaagtacta
    16201 cacattcttc agaactgctt atgagcgagc aatttaagca tttattggac tcagttaagc
    16261 atcaatatga catggttttg ttggatactg cacccattct tgcgattaca gatcccgcaa
    16321 tcatcggaaa atatgctggt acatcactct tagtggccta ttatagcgtg aataccgtta
    16381 aagaaattga acgcgcactt aaacatttca aacaaaataa tatcgaaatt actggcacca
    16441 tccttaatgg cattgatgaa aaatctgacg attatcatta tgtctaccag tattaataga
    16501 atggcgagcc acccctcgcc tttcgctctc ataccgacac aactaatgca tctttccaaa
    16561 tttgcgcgta aactctgcat catctttttc atcccgacca atagccgtta acgttatgcc
    16621 acatattgtt ttattcgaac ctgaaatccc accaaatact gggaatatca tccgcttatg
    16681 cgccaataca ggctttagtc tgcacctgat ccatcccctc ggttttactt gggatgacaa
    16741 acgcttacgc cgtgcagggc tagattacag cgaatttgcg gatatcaaac atcatcatga
    16801 ctattacgca ttcttagaaa gtgaagggtt aaatcctgat aataatcagt ctccaac
//
