LOCUS       AF498402               12953 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 011 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498402 AC104721
VERSION     AF498402.1  GI:20559790
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975250.
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="011"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27568.1"
                     /db_xref="GI:20559791"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             241..525
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27569.1"
                     /db_xref="GI:20559792"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEPE"
     CDS             554..808
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27570.1"
                     /db_xref="GI:20559793"
                     /translation="MEVFMRTLKRMLVGLLIVVVALSAIIFTLENRALIQFSFLGWET
                     PAIPSALLFSLVFVAGGLLGWFFGVFAGFAHHKSSRQPSV"
     CDS             919..1956
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27571.1"
                     /db_xref="GI:20559794"
                     /translation="MTADGEVDLVKLVKELWVNKVLILLTTLLALIGSFTYAYLSKPV
                     YEYRVAVVPPALGSIEGFNIGRRENGLDAYTVRSIYAIFSRNLLSDENKKEFFYKIYL
                     PQVGEGAESEDEQEEFYKKFSKEVKIDPANKPDADRYTVIVEGTKREVLATWAQAFVR
                     LAADRAVHEVIDSAGRDFQVRNAAMQSRITVLQNMAKGRRDDRIARLKEALLIAESLK
                     IDGPPLIEGASEQQLSSIMDGDLMYMRGAKALRAEINNLESRSVDAPFIPELRTLQEK
                     LSWNSSLSVDSDAVAVYKEDEGLSFSNQPIKPKKILIVTIGTLAGLIIGILLAVLAGF
                     ISKLRSDGSLR"
     CDS             2082..3278
                     /note="ORF_5; similar to Polysaccharide biosynthesis
                     protein; potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AAM27572.1"
                     /db_xref="GI:20559795"
                     /translation="MGSNYLLPLAAIPFLTRTLSSEAFGQLVIAQAVAVILCQLVDFG
                     FILAGSRKAAIIDNKVELSSFFSVVQSARFLLLLLSLLVLAILAVSSILPIPLLVLVA
                     AALPAVVGNYLQAVWFFQGRALFGWLALTNFLSKVFYFLLVVFFVTKDSDLVLASLGF
                     GFSYVIGGSALCCILFSMGIRWRPVLEKDRILDILRDGARSFLSLAFLSLHMQVLVAA
                     VGVVGGTSAAGVLSTADKFLRGIAAATSPIASALFPTFSRMYASADPAVGSLRRKALG
                     LMLLIAIPSCLFLFLFSEYISYLLFPEQSRGLTVVIRMFSIVPVFACIGVLYGGLTLV
                     PSGYDGVYLRAIFFAELGGVLTFILLALWGDEFFGAWTLVVTEVSLGMGMFLLATVKL
                     REKRGL"
     CDS             3278..4282
                     /note="ORF_6; similar to Glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpJ"
                     /product="glycosyltransferase"
                     /protein_id="AAM27573.1"
                     /db_xref="GI:20559796"
                     /translation="MLLSVVVSTWRRPKELKLVLAGLVAQSLAFKDFEVIVVDSNSGD
                     ETPDVVREFEAVQGFNVRLEQAELNSASAKRNKGVRLAKGRYVVLLDDDCVPDPAHLS
                     NFLNAANSRRGERVIWCGGVRFSCSLVSTSNYYRYRDSCHFSADKPLPNSLGYKTIVT
                     MNMLAERDILLQDNALFDERFVGYGFEDTEFGLRVGKLGYSVLPCRAEIEHVEPEGSI
                     SKFCRKYYHAARDGMPEFMKVTTTDDLETLGPIRWMEPGFVLSSKFQSLFRAVLCLAL
                     ESRFSNILIHSLEKTDSIRWLYSRAAYRLVLASSYRKGVLDRKSKLSKEKANKDGWYS
                     "
     CDS             4279..5514
                     /note="ORF_7; potential multiple membrane spanning
                     domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AAM27574.1"
                     /db_xref="GI:20559797"
                     /translation="MKQRSVALVLLFVFLFHILRNLSGAYYLVLAGFCGLFFLLFINN
                     WRDIKYSALGIFVIFFWTYASLVSVLWFDLYGDYSIGIFRLWASLPLLFVAMVLARDN
                     LKVPMQLISFFFSLAALSFLWQYVFGPIEWFAEASERAGGARFASLVGSLTAYGVMVG
                     VPALASLFYLKGWARVVCFFLLCLGAMLSLQKAALANIVIVLFFAYWLKLIKKTYLFF
                     TFLLAGLGLSVFFLADFGAGSLETVHRLIVGLLTSDSSVSQDVPFFESIFDRVTALPL
                     ETLSFYGSEILVLGAGVFGGGGVLGYAQYPMAHNGIVELLCIYGFIFGGLAVLLLLLL
                     FVYSVFLLVFRRRKIACTELGFLCSAFIIWFVNYVFSGGGLFHPVGAALFWLVVFRFL
                     YLLRRKRDVEHISKEVVYG"
     CDS             5507..6541
                     /note="ORF_8; similar to Polysaccharide biosynthesis
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /protein_id="AAM27575.1"
                     /db_xref="GI:20559798"
                     /translation="MDKNSVLLITGGTGSFGNAVLKRFLDTDIGEIRVFSRDEKKQDD
                     MRKCYAHPKLRFYIGDVRDYQSTLNATRGVDYIFHAAALKQVPSCEFYPMEAVKTNVI
                     GTENVLESAIQNGVKKVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNLERTPTVIC
                     GTRYGNVMASRGSVIPLFIEQMRSGQPLTITDPNMTRFMMTLTDAVDLVLYAFEHGTN
                     GDLFVQKAPAATIEVLAHALTQLLGKNGYPINVIGTRHGEKLYEALLSREEMACAEDM
                     GDYYRIPPDLRDLNYSKFVEQGEEKITHTEDYNSHNTKRLDIEGMKKLLLKLDFIRAI
                     QRGESASPEE"
     CDS             6545..7663
                     /note="ORF_9; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /protein_id="AAM27576.1"
                     /db_xref="GI:20559799"
                     /translation="MKVLVTGANGFVGRNLCAHLAERGGIEVVPFTRESSVGNLPELI
                     RSVDFIFHLAGVNRPEKPEEFKIGNSELTYDLCEAVRSNGRAIPLLYTSSIQAEVDNE
                     YGLSKRAAEEHLQVLGEDIGCPVYIFRLPNVFGKWSRPNYNSAVATFCHNIIRDIPIQ
                     INDSSAEITLVYIDDVVRTFMKVMDGKLSNAVSLQVEPQYQISVGELAEQLYEFRDSR
                     KSLTTARVGSGLTRALYSTYLSFLPEDSFSYDVPMHSDPRGTFVEMLKTADSGQFSFF
                     TAHPGVTRGGHYHHSKTEKFLVIKGMARFKFRNILTGAFYEICTNGEKAEIVETVPGW
                     THDITNVGTDDMVVMLWANEVFDRENPDTYACSVGEGA"
     CDS             7681..8811
                     /note="ORF_10; similar to UDP-N-acetylglucosamine
                     2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /protein_id="AAM27577.1"
                     /db_xref="GI:20559800"
                     /translation="MQKLKVVTVVGTRPEIIRLSRVMAKLDQYCDHVLVHTGQNYDYE
                     LNEIFFQDLGIRKPDYFLNAAGASGAETIGNVIIAVDRVLGEINPDALLVLGDTNSCM
                     AVLPAKRRKIPTFHMEAGNRCFDMRVPEEINRRIVDHTADVNLTYSTIARDYLLREGL
                     SPDMVIKTGSPMFEVLEHYRDGIESSDILERLGLKTERFFVVSAHREENIDSDKNFLK
                     LVSMLNAVAEKYSLPVIVSTHPRTKKRIEATEAKFHEGIKLLKPLGFKDYNKLQITAK
                     AVISDSGTISEESSILNFPALNIREAHERPEGMEEAVVMMVGLDSDRVLQALEVLEGQ
                     RRDAERMLRLVADYSMPNVSEKIVRIVHSYRDYVMRTVWKKY"
     CDS             8829..10031
                     /note="ORF_11; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr1"
                     /product="glycosyltransferase"
                     /protein_id="AAM27578.1"
                     /db_xref="GI:20559801"
                     /translation="MARIFVVSEYVGANQNSTGYYWEKIIGKMQREFGGLTVIFPLTA
                     GETPPVVSPSVEQECFKFPRSNKNRLLSRGLAQIFQAFLFSVKLTSRARRGDVVLSGT
                     NPALLLMTFPLLRYALGFKWVLLVHDVFPENLVPAGVLKKDSIAYRLLRRLFSFIYSS
                     ADRLVVIGRDMEALMKEKVNDPRSLVFISNWACEKEVFPVPREDAPFINIPEWKGKRV
                     FQFFGNVGRLQGIENILSAIQLVKNEKAAFAFIGDGALVDSVKKHALEDQCARLRYFG
                     RLPLAEKNFGLAACDVALVTLEEGMFGLGVPSKAYFSMAADKPILAVMEKGAEISRII
                     DETGIGWSCPPNDPVALARLIDEICELDLSSLGGVPRSVLQQNYSEYISLEKFAACVR
                     PLLSESKI"
     CDS             10031..10972
                     /note="ORF_12; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /protein_id="AAM27579.1"
                     /db_xref="GI:20559802"
                     /translation="MKVLVTGASGFVGSALCRSLAAGPFQVVGQVRSLCNPVTGVEYV
                     RAELKESTKLDAALRGVECVVHLAGRAHIFGRQRDSLDIFRKVNRDATLALARQAIEA
                     SVKRFIFVSSIGVNGALTKEKPFDENSKPAPHAEYAISKFEAEVALRELFKQSSTELV
                     IVRPPLVYDWNAPGNFSRLLKLVASGLPLPFGCIDNRRSFVSLDNLVDFLACCMTHPS
                     AAGELFLVSDGQEISTKQLVTALATGMGRRPIMWPVPRFILRFLKLVGKGGLYTQLCC
                     SLEVDSSKGRFLLGWEPRKSTLSALEDVGRIYVKRTE"
     CDS             11073..12098
                     /note="ORF_13; similar to Glycosyl transferase group 4;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27580.1"
                     /gene="gtr2"
                     /product="glycosyltransferase"
                     /db_xref="GI:20559803"
                     /translation="MEEWYLLLAAAGVSGLLTGLLRRYALARSLLDTPNSRSSHVVPT
                     PRGGGVAIVVTFCLMLPIWAVLGNISWAVSWALLLAGGGVAIIGFMDDHGHIAARWRL
                     LGHFSAALVSLYFLNGIPPFQIVGVSWDLGWFGGLLFAFYLVWLLNLYNFMDGIDGLA
                     SLQAIFVCVGGALLYWLNGQLTQALLPLSLAFAVFGFLFWNFPPAKIFMGDAGSGFLG
                     IVLGILSIHAMWMNTNFFWAWLVLLGVFIVDATYTLIRRLLRGDKVYEAHRSHAYQYA
                     SRYYGKHAPVTIGVTALNVIWLLPIALLVGSGSLEPLMGIVIAYVPLVFLAVRFKAGK
                     LESSAQA"
     CDS             12144..>12953
                     /note="ORF_14; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpM"
                     /protein_id="AAM27581.1"
                     /db_xref="GI:20559804"
                     /translation="MPRRWKRLLQVVTDILLVWLSLWLAFVVRLGTDDMIDVFGEHAW
                     LFITAPVIAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSLVVYWYRGAPAP
                     VPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDGLPRVAIYGAGA
                     AGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMIDETGAQEVLLA
                     IPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDIADLLG"
BASE COUNT     2880 a   2685 c   3501 g   3887 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgta agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga tctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 gaaaaagggc ggcctaggcc gccctttttc gttttcccct tcttggacct gttcaaagac
      181 tgatcagcat gctaaaagag acctgagctg atctagccgc ttgaaaaaga agggaaaacc
      241 atgaccaagt cggagttgat cgaacggatc gttacccatc aggggcaact gtccgcgaag
      301 gatgtcgagt tggcaatcaa gaccatgctg gagcaaatgt cccaggcctt ggcgaccggg
      361 gaccggatcg agatccgtgg tttcggcagc ttttccttgc actaccgcgc cccgcgcgtc
      421 ggtcgcaacc ccaagaccgg ggagtcggta cgactcgacg gcaagttcgt gccgcacttc
      481 aagccgggca aggagttgcg ggatcgggtc aacgagccgg aataaggctg tttagaggct
      541 tgacctgctt ggtatggagg tattcatgcg cacgcttaaa agaatgcttg ttgggctact
      601 gatagtggtt gtggccttgt cggcaattat ttttactttg gaaaatcgtg ctcttatcca
      661 attttccttc ttggggtggg agacgcctgc gattccgagt gcattgttat tttcattggt
      721 gtttgttgcg ggcggtctgc tcggttggtt ttttggagtt tttgcaggct ttgcgcatca
      781 taagtcgtca agacaacctt ccgtgtagaa cttgtcgggg atcgtggtgt ccaacccatc
      841 ttgttgggct tttcccctga tagagtttcc gattggactg atgatggact gaatgtgaat
      901 aagcagaatt cttctttgat gacggctgat ggtgaggttg acctggtgaa gctggtcaag
      961 gagctttggg ttaacaaggt tctgattctt ctgactactc ttcttgcatt aatcgggtct
     1021 tttacctatg cgtatctgag taagcctgta tatgaatata gggttgcagt agtacctcct
     1081 gctcttgggt ctatcgaagg tttcaatatt ggtagaaggg agaatggcct agatgcatat
     1141 actgttagaa gtatctatgc gatcttttcg cgcaatctgc tttcggatga gaataaaaaa
     1201 gagttcttct ataagatata ccttccccaa gtgggtgagg gagcggaaag cgaagatgag
     1261 caggaggagt tttataagaa gttctccaaa gaggtaaaga ttgatcctgc taacaagcca
     1321 gatgcagacc gttatacggt aattgtggag ggcacgaagc gagaggttct tgctacatgg
     1381 gcacaagctt tcgtgcgttt ggctgcggat cgggccgtgc atgaggttat tgatagtgca
     1441 ggtagagatt tccaggtaag aaatgctgca atgcagagcc gcataaccgt gctgcagaat
     1501 atggcgaagg gccgccgtga tgataggatt gcacgtttga aggaggcatt gctgattgcg
     1561 gagtcgctca agatagatgg cccgccatta atagaagggg cgtccgagca acaactttcc
     1621 tcgatcatgg atggtgactt gatgtacatg cgaggagcta aggcgctgcg cgctgaaatc
     1681 aacaatcttg agtcgcgtag tgtagatgct ccattcattc ctgagttgag aactctccaa
     1741 gagaaactat cttggaactc cagtttgtct gtggattctg atgcggtcgc tgtctacaag
     1801 gaagacgagg gactctcttt ttcaaatcaa cccattaagc cgaagaagat tcttatagtt
     1861 actataggta ctttggcagg attgataatt ggaattctac tcgcagtgct cgctggtttt
     1921 ataagtaagc ttcgtagcga tggctcgctt cgctaagttt tgatctaaac cctgatggca
     1981 cttactggca tcagggttta cttgttgtag ttgttgaatt gtattagggg gtgtcgagta
     2041 gaatgtcaat aaggtggggc gttttctact ccggtatatc catgggcagc aactatcttt
     2101 tgcctcttgc tgcgattcct tttctcacta gaacattgtc aagcgaagcg tttggccaat
     2161 tggtgattgc ccaggccgtg gctgtcattc tatgtcaact ggtagacttt ggatttattc
     2221 tggcaggatc aagaaaggct gccattatcg ataacaaagt tgaactgtct agtttctttt
     2281 ctgttgtaca gagtgctaga ttcttattgt tgctgctttc acttttagtg ctggccattt
     2341 tggctgtatc ttctatttta ccaatcccct tgcttgtatt ggttgcggct gctcttccgg
     2401 cagtagttgg aaattatctt caagcagtat ggttctttca gggaagagcg ctgtttggat
     2461 ggttggcgct taccaacttt ttgtctaagg tgttttattt cctattggtc gttttttttg
     2521 tcacgaagga ttctgacctt gtactggctt cgttggggtt tggtttttcc tatgtcatag
     2581 gtggaagtgc tctctgttgt attttatttt ctatgggaat acggtggcgc ccggttctcg
     2641 agaaagacag aattctcgat atattgcgtg acggtgctcg atcttttctt tctctggctt
     2701 ttcttagctt gcacatgcaa gtgctcgttg cggcggttgg tgttgttggt ggaacctccg
     2761 cggccggagt gctttctact gcggataaat tccttcgcgg gatcgcggct gctacttcac
     2821 ccatagctag cgctctattt ccgactttta gcaggatgta tgcgagtgcc gacccggcag
     2881 tcggcagttt aagaaggaaa gcgctaggtc tgatgttact aatagctatt cctagttgtt
     2941 tatttctttt cttattttct gaatacattt catatctcct atttccggaa cagtccagag
     3001 ggctaactgt tgtaataaga atgttttcga tagtgccagt gtttgcttgt attggtgttc
     3061 tgtatggagg gttgactctt gttccttctg ggtatgatgg tgtatatttg cgagcaattt
     3121 tttttgcgga attgggcggg gtattaacat ttatcctctt ggcgctttgg ggggatgagt
     3181 tttttggagc gtggacgcta gtcgttacag aggtctcttt ggggatggga atgtttttgc
     3241 tggccacggt taagttgaga gagaaaaggg gactttgatg ttgttgagtg tcgtcgttag
     3301 tacttggcgt cgtcccaagg aactaaaatt ggtcctggcc ggccttgtag cgcagtcact
     3361 ggcttttaag gattttgaag ttattgttgt cgacagtaat agtggtgatg aaacacccga
     3421 tgtggtccgc gagttcgaag cggttcaggg cttcaacgtc cgtcttgagc aggccgagct
     3481 gaattcagct agtgcaaaac gaaataaagg cgttcgttta gcgaaggggc gctacgtagt
     3541 gcttcttgat gatgattgtg tgccagatcc tgctcatctc tcgaattttc tgaacgctgc
     3601 taactctcga aggggggaac gcgttatctg gtgcggtggt gtcagatttt cttgctcatt
     3661 ggtgtcgacg agtaattact atcgctatag ggatagttgt cacttctctg ctgacaagcc
     3721 gttgcccaac tccttggggt ataaaactat cgttaccatg aatatgctcg ctgagaggga
     3781 tattctgctc caagataatg ctctttttga tgagcgattt gttggatacg gctttgaaga
     3841 tacggagttt gggttaaggg ttggcaaact tggttattct gttcttcctt gtcgggctga
     3901 aatcgagcat gtcgaaccgg aaggcagtat ttcgaagttc tgtcgcaagt actatcatgc
     3961 cgcccgtgac ggaatgccgg aattcatgaa agttacaaca actgatgacc ttgagactct
     4021 tggccctata cgatggatgg agccgggctt tgttttgtca agcaagtttc agagtctatt
     4081 cagagcagtg ctgtgtcttg cgctggagtc aaggttttca aatattctta ttcattctct
     4141 agagaaaacg gactctattc gatggctcta ctctagagct gcttataggc tcgttttggc
     4201 ctcatcctat cggaaggggg ttctcgatag aaagtcgaaa ctttccaaag agaaagcaaa
     4261 caaagatggg tggtactcgt gaagcagaga agcgttgctc tggttctcct ttttgtgttt
     4321 ctctttcata ttctccggaa tctatccggt gcgtattatt tagttcttgc tgggttttgt
     4381 gggctgtttt ttctcttatt tattaataac tggagggata ttaaatatag cgcattagga
     4441 atatttgtta ttttcttctg gacgtatgcc tcgctcgttt cggtattatg gttcgatcta
     4501 tatggagact attccatagg gattttcagg ttgtgggcat cattacctct tctctttgtt
     4561 gctatggttc ttgccagaga taatctaaaa gtgcccatgc aacttatttc atttttcttc
     4621 agtctggcgg ctctttcttt cctctggcag tatgtatttg gacctataga atggttcgct
     4681 gaagccagtg agcgtgctgg gggcgcccgt tttgcatcat tggtaggaag tttgaccgca
     4741 tatggtgtta tggtaggagt tcccgcactt gcttcactct tttatctaaa gggttgggcg
     4801 cgcgtggtat gtttctttct tctttgcctg ggggcgatgc tgtcgctgca gaaagctgct
     4861 ctggccaata tcgtcattgt cttattcttt gcctattggc taaaactgat caaaaagaca
     4921 tacttgtttt tcaccttcct tcttgcaggg ctgggtttat ctgtattttt ccttgcagac
     4981 ttcggggctg gcagtctgga aaccgtgcat cgactgattg tcgggctcct cacctccgat
     5041 tcctcagtct cgcaagatgt tccattcttc gaatccatat tcgatcgggt aactgcactg
     5101 cctttggaaa cattgtcttt ctacggtagc gagatacttg ttttgggcgc gggcgttttt
     5161 gggggagggg gggttctagg gtatgcgcaa tatccaatgg cgcacaatgg tattgtcgag
     5221 ctgctttgca tctatggttt catttttggt ggcttggctg ttcttttgct attgttgctt
     5281 tttgtctact ctgtattttt gcttgtcttc cggcgaagaa aaatagcttg tacagagtta
     5341 ggcttcttat gctctgcatt tataatctgg ttcgttaatt atgtctttag cggcggtggg
     5401 cttttccatc cggtaggggc tgcattgttc tggctcgttg tttttcgctt tctttatctt
     5461 ttgaggcgga aaagggacgt tgaacacatt agtaaagagg tggtgtatgg ataagaactc
     5521 tgttctgtta attaccggtg gaactggttc ctttggaaat gccgttttga agcgttttct
     5581 ggatacagat attggtgaaa tacgtgtatt cagtcgggat gagaagaagc aagatgatat
     5641 gcgtaagtgc tatgctcacc ccaagttgag gttttatatt ggagatgtcc gcgactatca
     5701 aagcactctg aatgctactc gcggtgtgga ctacattttc catgcggctg cattgaaaca
     5761 ggttccctct tgtgagttct acccgatgga ggctgtgaag accaatgtca tcggaacgga
     5821 aaatgtcctc gaatctgcta tccagaatgg cgtcaaaaaa gtcgtttgtc tgagtacgga
     5881 taaggcagtt tacccaatca atgccatggg tatttcaaag gccatgatgg aaaaggtcat
     5941 ggtcgccaag tctcgaaacc tggaacgcac tcctaccgta atttgtggca cccgttatgg
     6001 taacgtcatg gcttcaaggg gctcggtcat tcctctcttt atcgagcaaa tgcgatcagg
     6061 ccagcctctc acaatcactg atccaaacat gacacgtttc atgatgacgc ttacggatgc
     6121 cgtagacctt gtgctttatg ctttcgagca tggtaccaat ggagatcttt ttgtacagaa
     6181 agcaccggct gcaaccatcg aggtgctggc tcatgcgctt actcaattgc ttggcaagaa
     6241 tggttatcct atcaatgtaa taggtacgcg tcatggagag aagctttatg aggcgcttct
     6301 tagtcgagag gaaatggcct gtgctgaaga catgggtgac tattatcgta tccccccaga
     6361 tttgcgtgat ctgaactata gtaagtttgt ggagcaaggc gaggaaaaaa ttactcatac
     6421 agaggactat aattctcata ataccaaacg tctggatatc gaaggaatga aaaagctgtt
     6481 gctgaagttg gatttcattc gtgctattca gcgtggcgag agtgccagtc cagaggaata
     6541 aacgatgaaa gttcttgtaa ctggcgcgaa tggatttgtt ggaaggaatc tgtgcgctca
     6601 tcttgcagag cggggtggta tcgaggtggt gccattcacc cgcgagagta gtgttggtaa
     6661 tttgcctgag ctaattcgtt ccgtcgattt tatttttcat cttgccgggg tcaatcgtcc
     6721 ggaaaaacca gaagagttta agatcgggaa ttccgaactc acgtatgatc tgtgtgaggc
     6781 ggtaaggtcc aatggacgag ccataccact tctttatact tcatccattc aggctgaggt
     6841 ggataatgaa tacggtttaa gcaagcgagc cgcagaagag catctccaag tgctaggtga
     6901 ggatattggt tgtcctgtct acatatttcg ccttcctaat gtattcggta aatggtcgcg
     6961 tccgaattat aattcagcgg ttgcgacttt ttgtcataat attattcgag atattccgat
     7021 tcaaattaac gattcctcgg cagagatcac tcttgtatac atagatgatg tggttcgcac
     7081 cttcatgaaa gtcatggatg ggaagctatc caatgcagtt tcactacagg tcgagcccca
     7141 gtatcagatt tctgttggtg agctcgcaga acaattgtat gagtttcgtg atagtcgaaa
     7201 gtcactgact accgcaaggg ttggctcggg attgacgcgc gccttgtact cgacttatct
     7261 aagtttcttg ccagaagata gttttagtta cgacgtgcca atgcattcgg atccgcgtgg
     7321 cacattcgtc gagatgctga agaccgcgga ctctggccag ttctcgtttt ttacggctca
     7381 tccaggtgtt accaggggcg ggcattacca tcactcgaaa accgaaaagt ttctggttat
     7441 caaagggatg gcacgtttca agtttagaaa catcctgacc ggggcatttt acgaaatttg
     7501 cactaatggt gaaaaggcag aaattgtcga aacagtacct ggatggactc atgacattac
     7561 taatgtcgga actgacgata tggtagtcat gttgtgggct aacgaagtat ttgatcggga
     7621 aaatccggat acctacgctt gttcagtagg cgaaggtgcg taaggtatag tgagataaca
     7681 atgcagaagc taaaagtcgt tacggttgtt ggaactcgtc ctgagattat tcgcttgtct
     7741 agggtcatgg cgaagcttga tcagtactgc gatcatgtac ttgtccatac tgggcagaat
     7801 tatgattacg aacttaatga aatatttttt caggacctcg gcataagaaa gccggattat
     7861 tttctaaacg ccgccggggc ttccggggct gaaacgatag ggaatgtaat aatcgcagtc
     7921 gatcgtgttc tgggcgaaat caatcccgat gcactgctcg tgctgggtga taccaatagt
     7981 tgtatggcgg tactgcctgc aaaacggcgt aagataccga cctttcatat ggaagcaggc
     8041 aatcgctgtt tcgatatgcg tgtgcctgaa gagataaatc ggcgcattgt cgatcataca
     8101 gctgatgtaa atttgaccta tagtacaatt gcgcgtgatt atctcttgcg tgaaggactt
     8161 tctccagaca tggttatcaa gactggtagc cctatgttcg aagttctcga gcactatcgt
     8221 gacgggatcg agtcctccga tattcttgaa aggctcgggt tgaaaacaga gcggttcttt
     8281 gtcgtgagtg cgcaccgaga ggaaaacata gattcggata agaacttctt gaagttggtt
     8341 tctatgctca acgctgtggc agaaaagtac tcgctgcccg tcatcgtatc aactcaccct
     8401 agaacaaaaa agagaattga ggcgacggag gcaaagtttc acgagggtat taaactgctg
     8461 aaacccctcg gctttaagga ttacaataaa ctgcaaatta cagccaaggc agttatttct
     8521 gacagtggga ccatcagtga ggagtcttca atactgaatt ttcccgcttt gaatattcgt
     8581 gaggctcatg aacgcccaga aggcatggaa gaggctgtgg tgatgatggt cggactggat
     8641 tcggatcgag tactacaagc actcgaggtg ttggagggac agaggcgcga cgcagagcgc
     8701 atgttacgct tggtcgctga ctatagcatg cccaacgttt ctgaaaagat tgttcgcata
     8761 gttcatagct atcgggatta tgtcatgcga actgtctgga aaaaatatta acttgaggcg
     8821 tggagttgat ggcaaggata tttgtggttt ctgagtatgt cggtgccaat cagaactcca
     8881 cgggatacta ttgggagaag ataataggaa agatgcagcg ggagtttggt gggctaaccg
     8941 taattttccc gctgaccgca ggtgaaaccc cgcctgtggt ttcaccttcc gttgagcaag
     9001 aatgctttaa gtttccgagg agcaataaga ataggctcct ttctagagga ttagcgcaga
     9061 tttttcaggc gtttctgttc tcagtaaaat tgacttctcg tgccagacga ggagatgtgg
     9121 tattgagtgg aaccaaccct gctcttctac tgatgacgtt tcccttgcta aggtatgccc
     9181 tcggtttcaa gtgggtgctg ctggtgcatg atgtatttcc cgagaacttg gtgccggcgg
     9241 gcgttctgaa gaaagatagt attgcctacc ggcttctacg tcgtctcttt tctttcattt
     9301 actcatccgc tgatcgtcta gtcgtaatag ggcgcgatat ggaagctctt atgaaagaga
     9361 aggtgaatga cccgcgatct ttggtcttta tttcgaattg ggcctgtgag aaagaggttt
     9421 tcccagtacc gagagaggat gctcctttta tcaatattcc tgaatggaaa ggtaaaaggg
     9481 ttttccaatt ttttggtaat gtcggtcgat tacaaggtat agaaaacata ctttctgcta
     9541 ttcagttggt taaaaacgag aaggcggctt ttgcttttat tggagatggt gccttggtcg
     9601 acagtgtaaa aaaacacgcg ctggaagatc agtgtgctcg gttgaggtat tttggaaggc
     9661 tgccattagc cgaaaagaat tttggtttgg ctgcctgtga cgttgcctta gttaccttag
     9721 aagaaggaat gttcgggctt ggggttccca gcaaggcata tttctccatg gcagcagaca
     9781 aaccgattct agctgtcatg gaaaaagggg ctgaaatctc ccgtataata gatgagaccg
     9841 gaatcggttg gagctgtccg ccgaatgatc cggttgcttt ggcaagattg atcgatgaga
     9901 tttgtgaact cgacttgtct agtttaggcg gagtcccgcg gagtgtcctt cagcaaaatt
     9961 attctgaata tatttcattg gaaaaattcg ctgcctgtgt tcgaccgctt ctgtctgagt
    10021 cgaaaatatg atgaaggtgc tggtaaccgg ggctagcggt tttgtcggga gtgcgctttg
    10081 caggtcgctt gctgccggcc cctttcaggt tgtcggacaa gtacgatccc tgtgcaatcc
    10141 tgttacgggg gttgagtatg ttcgagcgga gctgaaagag agcactaagc ttgatgctgc
    10201 gctgcggggt gttgaatgtg tagttcatct agctggacga gcccatatct ttggaaggca
    10261 gcgtgattca ctagatattt ttcggaaggt gaatcgcgat gctactctgg cgcttgctcg
    10321 gcaggcgatc gaagcatctg taaagcgttt catttttgtt agttctattg gtgtaaatgg
    10381 cgctttaacc aaagaaaagc ccttcgatga gaactccaag ccggctcctc atgcagaata
    10441 tgcgatttca aagtttgagg ctgaagtagc gcttcgggag cttttcaagc agtcctcaac
    10501 agaacttgtt atcgtcaggc ctccactcgt ttacgactgg aatgctcctg gaaatttctc
    10561 gcgattgttg aagctggttg cttcgggact tcctcttccg tttggttgca tagataaccg
    10621 acgaagtttt gtttctctgg ataatttagt tgactttcta gcttgctgta tgacgcaccc
    10681 ttctgctgcc ggcgaactgt ttttggtatc cgatggtcag gagatttcta ccaagcaact
    10741 ggtgactgcg cttgctacgg gaatggggcg tcgccccatc atgtggcctg ttcctaggtt
    10801 tattctgagg tttcttaaat tagtaggaaa gggtgggtta tacactcagt tatgctgctc
    10861 actagaggtc gactcgtcga aaggcaggtt tttgcttggt tgggaacccc gcaagagcac
    10921 cctttccgcg ttggaagatg ttggtagaat atatgtcaaa cgtactgaat gattatctgc
    10981 aggcgctttg ctactagcat ggcgtaccac gcagaacaat cgaatagaac cctgttgaag
    11041 gggtgagagt attttttggg ataaatttat aaatggaaga atggtatttg ttactcgctg
    11101 cagctggggt ttcgggactg cttacaggcc tcttgcgtcg ttatgcctta gcgaggagct
    11161 tacttgacac ccctaactct cgaagttccc atgtcgttcc cactccacgc ggaggagggg
    11221 tcgccattgt agttactttt tgtctcatgc tgcctatttg ggctgtactg ggaaatatct
    11281 catgggccgt gtcctgggct ttacttctcg ctggcggcgg ggttgccatt attggattca
    11341 tggatgatca cggtcatatc gccgcacgct ggcgtctgct gggacatttt agtgcagcct
    11401 tggtctcatt gtactttttg aatggcatac caccatttca gattgttggt gtcagttggg
    11461 acctggggtg gttcggagga cttctctttg ctttctatct cgtgtggttg ctgaatctct
    11521 ataacttcat ggatgggatc gatggacttg ccagccttca ggccattttt gtctgtgttg
    11581 gcggggcatt attatactgg ctgaatggcc aactgacgca ggctttgctc cccttatcgc
    11641 tagcttttgc cgtttttgga ttcttgttct ggaattttcc acccgcaaaa attttcatgg
    11701 gagatgcggg tagtggtttt ctggggattg ttttaggaat tctttccatt catgccatgt
    11761 ggatgaatac gaattttttc tgggcatggt tggtcctgtt aggcgttttc atcgtcgatg
    11821 cgacctatac cctgattcgt cgcttgctga gaggggacaa ggtgtatgag gctcatcgaa
    11881 gccatgccta tcaatacgca agccgatact atggaaagca tgctcctgtt acgattggcg
    11941 tcacggcatt gaacgtcatc tggctcctcc ctatagcctt gttggtcggg agtggatctc
    12001 tagagccttt gatgggcatc gtcatagcct acgtccctct cgtttttctg gcagtgaggt
    12061 tcaaggcggg taagctagag tcgtccgctc aggcctaaag gagtagggga atgctagatc
    12121 gtttaagagt aaagttgtta tccatgcctc gtcgctggaa acgtttgctt caagtggtta
    12181 cggatatcct tctggtatgg ctgtctctgt ggctcgcttt tgtggtccgt ctaggcacag
    12241 acgatatgat cgacgtgttc ggcgagcatg catggctttt catcactgcg ccggtcatcg
    12301 ccattccact attcattcgc ttcggcatgt atcgcgcggt gatgcgttat ctcggtaacg
    12361 acgcattgat cgccatcgcc aaggcggtga ccatctcggc tctggtgctg tcgctggtgg
    12421 tgtactggta tcgtggcgcg ccggcgccgg tgccgcgttc cctggtgttc aactactggt
    12481 ggttgagcat gctgctgatc ggcggcttgc gtctggccat gcgccagtat ttcatgggag
    12541 actggtactc tgctgtgcag tcggtaccat ttctcaaccg ccaggatggc ctgcccaggg
    12601 tggctatcta tggcgcgggg gcggccggca accagttggt tgcggcattg cgtctcggtc
    12661 gggcgatgcg tccggtggcg ttcatcgatg atgacaagca gatcgccaac cgggtcatcg
    12721 ccggtctgcg ggtctatacc gccaagcata tccgccagat gatcgacgag acgggcgcgc
    12781 aggaggttct cctggcgatt ccttccgcca ctcgggcccg gcgccgagag attctcgagt
    12841 ccctggagcc gttcccgctg cacgtgcgca gcatgcccgg cttcatggac ctggccagcg
    12901 gccgggtcaa ggtggacgac ctgcaggagg tggacatcgc tgacctgctg ggg
//
