LOCUS       AF498404               13401 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 013 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498404 AC104723
VERSION     AF498404.1  GI:20559835
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 13401)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975252.
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="013"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27608.1"
                     /db_xref="GI:20559836"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             241..525
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27609.1"
                     /db_xref="GI:20559837"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEPE"
     CDS             597..815
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27610.1"
                     /db_xref="GI:20559838"
                     /translation="MVALTVIIFVLENQSGVNLSFLGWRTPEGPLSLFVSLAFVLGGL
                     GGVLSGMWLRLIGSVSKGHLPVRREAMK"
     CDS             1167..2216
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27611.1"
                     /db_xref="GI:20559839"
                     /translation="MTEETFFQRVAKNEEFDLIGLLRGLWAEKWLIACITILTTSLVG
                     TYAFLSKPVYEARIAVLPPSWSSIAGFNLGRAENGLTTFSVNDVYSVFLRNLLAEETR
                     RNFFREVYLPSLSENERSGSKDRLYSNLSEEMEIQRPSKDERERYTVIFEKHDPGQAA
                     DWGRRYIKLAAEGSMKEMQDAVQREFEVKSRNIQQQIDILRDAAKARREDRINRLKEA
                     YQIAESLKLENPPLIGGQMDQQLSSIMEGALMYMRGTKALRAEIRALEERTSDDPFIP
                     ALRNLQEKQAMYSTLKLDVNKVAVFRQDGVAEVPDDPVRPKKGILLVLGALSGLVWGV
                     MIAFIVMAVRRSRAK"
     CDS             2303..3568
                     /note="ORF_5; similar to UDP-glucose/GDP-mannose
                     dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpA"
                     /protein_id="AAM27612.1"
                     /db_xref="GI:20559840"
                     /translation="MDIKAAVVGLGYVGLPLAVEFGKKRSVTGFDINHSRIRDLQKGY
                     DSTLEVEKEELESAVHLKFTSTLSELQECNFFIVTVPTPIDEHKQPDLTPLVKASESI
                     AKVLKKNDIVVYESTVYPGATEEVCVPVLERESGLRFNSDFFVGYSPERINPGDKEHR
                     VSTIKKVTSGSTPEVAEIVDSLYREIITAGTHKAESIKVAEAAKVIENTQRDLNIALI
                     NELAIIFNKLEIDTESVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQSIGYHP
                     EIILAGRRLNDGMGAYVVSQLVKAMLRKRIHVDGARVLVMGLTFKENCPDLRNTKVVD
                     IVRELAEYNIAVDVYDPWVSVEEAQHEYGLTPISAPVEGNYDAVVLAVAHNEFKELGA
                     DKIRAFGKLESVIYDLKFILDKSDSDLRL"
     CDS             3578..4603
                     /note="ORF_6; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /protein_id="AAM27613.1"
                     /db_xref="GI:20559841"
                     /translation="MNAYAKALSRLETTTSSWLVTGVAGFIGSNLLELLLKFNQKVVG
                     LDDFSTGHMYNLDEVRGLVGEERWRNFKLIQGDIRDIGDCRRAVEGVDYVLHQAALGS
                     VPRSIADPITTNSVNISGFLNILVASKEAGVKSLTYAASSSTYGDHPGLPKVEEVIGK
                     PLSPYAVTKYVNELYADVFSRCYGFNLIGLRYFNVFGKRQDPNGAYAAVIPKWISAMI
                     KGEPVYINGDGETSRDFCFINNVIKANLLAAFCETEEAKNQVYNVAYGGRTTLGQLFD
                     SLKVELSRNGINYEQGPIYREFRAGDVRHSQAEIKKAQAYLGYNPEYDIQAGIAEAMP
                     WYISFVK"
     CDS             4665..5954
                     /note="ORF_7; similar to Polysaccharide biosynthesis
                     protein; potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AAM27614.1"
                     /db_xref="GI:20559842"
                     /translation="MNPKNIFWNLLGLGLPLIIAALTVPHLIATVGVERFGFLALSWG
                     LIGYAGILDLGIGRAVTQKLSSIRGSDEEMNSYTIMSTAIRITAIVGGLGFSLIIIFG
                     LLGGGEFFSHKDVSSLELEFSVLLLAFALPLQAISATYRGVNEAYLNFKGINLLRIFL
                     GATNFGGPFLVSFYAKDLHYLVTTLVLSRALAFFIFRRLAHGVLKETLVERSCKYDRR
                     QAVELFRFGGWVTVSSIINPFLVQSDRFFIGVLLSAAAVTSYVIPYEITIQSMILVGA
                     VSTVAFPSISNLIRTSFAEALECFNKWLLRVLLIMGGGMLCLAFLLPFILKLWVGDYI
                     GDDSISVGRILCLGVFFNALGAMFYSFLHANAKVKETAILHSIELPIFILILIILIPR
                     HGIVGAAVAWSLRTLVDTIVLAVLSYFSGWRVKFANC"
     CDS             6001..7176
                     /note="ORF_8; similar to Sodium/hydrogen exchanger family;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AAM27615.1"
                     /db_xref="GI:20559843"
                     /translation="MVFPTALIGIKFILLILALLFTLISSLARADLNLYVLLAGLFYS
                     GVGLVWSIYGELRGNAGALQVLTVMVVYPVLFVLLAVSYRGQSEALGKLFFYAALLIA
                     VLDIFFVFGNYLFSYNPVYESMAAMYGDAAVVDNAGRYIKFTLPNVSSLIFLIPYVVS
                     YLLCRKFSLSGLFLLALLLLVAFVSGRRALLLVAFTAPFLAYFLTMGADRFQWKVISR
                     VLLVFIVFMLGFLIFYNTYPEYVENLFLSISNFSDNDSNLERRYQFEALMKGVYQQPF
                     FGMGAGAIADYIRSEEMPWAYELFYVSLVFQYGILGFLFYALGVLFLLVFLVLRVRKI
                     GRRSFEFCYLSGMLAFLMATATNPYLAKFDYMWVIFIPVALLNYSAVSRGKDGKSNY"
     CDS             7157..8092
                     /note="ORF_9; similar to Glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr1"
                     /product="glycosyltransferase"
                     /protein_id="AAM27616.1"
                     /db_xref="GI:20559844"
                     /translation="MESPIIDVVVVNWNAGLQLRACVDSIFKNSSGLIRKVIVIDNGS
                     NDNSCALLGERENLEVVLTGENLGFATACNLGASKGKAEYILFLNPDAELYEETIGSV
                     VRFMNRSENSKVGVCGVQLIDEQGQVSRSCARFPSSARMLMHTMGVDRIFPALGYAMT
                     DWDHLSSRPVDHVIGAFYFVRRHVFKLVGGFDERFFVYLEDLDLSYRISCSGWSSFYL
                     STAQAFHAGGGASRQIKARRLFYSLRSRLIYIFKNLPTVSAFLILLSSLFFEPVIRSM
                     FSMVKLSFPALRETWSAYLMLYRWLPKWIFFNATR"
     CDS             8098..9168
                     /note="ORF_10; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr2"
                     /product="glycosyltransferase"
                     /protein_id="AAM27617.1"
                     /db_xref="GI:20559845"
                     /translation="MKILLLSKYPRLGASSRLRTLQYIDYLESKGGRVDVRSFFDDGY
                     LSGLYSSGRRSFYNVLKSYISRFFVLFTVCRYDLIWIEKEIFPYFPSVAERFLRFFGK
                     PYVVDYDDAIFHNYDLSGNWLVRKFLGKKIDSVMRHSSCVIAGNDYLAERARVAGSSR
                     VEIIPTVIDLERYEVRDESCSKKIVIGWIGSPSTQKYLLDIAPELVKLCVEYQASMLL
                     VGATPEISSYFSGVYVDVKAWKEEDEVGYIRQMTIGIMPLPDGPWEKGKCGYKLIQYM
                     ACGVPVVASPVGVNIEIVENNESGLLAEGNRGWRESLQKLLQSSELRRECGRNGRSSV
                     EKIYSLQVQSRRLHQTFTEVLS"
     CDS             9611..10378
                     /note="ORF_11; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr3"
                     /product="glycosyltransferase"
                     /protein_id="AAM27618.1"
                     /db_xref="GI:20559846"
                     /translation="MAKGSKASVFRSGIAWLYRRALGKKRLRVIFQNPDDRDALIGLG
                     AITYEKSVLIRGSGVDLSLCHPAPESPGTPVVTLAARLLRDKGVVEFVEAANILRQRG
                     VSAHFQLVGDLDPGNPSSIEEFDLERWRSEGTVECLGYRQDIASVFAHSHIVVLPSYR
                     EGLPKVLVEAAACGRVVVTTDVPGCRDAIEADRTGLLVPVRDAVALADAIQRLVESPE
                     LRKKMGAAGRTLAERDFAIESIVQQHLDIYRALGSGA"
     CDS             10396..11337
                     /note="ORF_12; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27619.1"
                     /gene="orf9"
                     /db_xref="GI:20559847"
                     /translation="MTGATGFVGAALVESFWSSEKYRVWAGYRRESSPWPEGVTPLFL
                     GELGSSVAWETESLIDTVIHCAARVHVMSETVSDPLAEFRKANVQGTLDLARQAASLG
                     VRRFIYLSSIKVNGEGSEPGQPYFADSLPNPVDPYGVSKLEAERALLDLAAETGLEVV
                     IIRPVLVYGPGVKANVQTMMRWLKRGVPLPLGAIHNRRSLVALDNLTDLIATCIDHPA
                     AVNQVFLVSDGEDLSTTDLLRRMGNALRTPARLVPVPASWIGLVAKTLNKQAFAQRLC
                     GSLQVDIEKNRRMLGWTPPVGVDEALEKTARYFLDHQ"
     CDS             11334..12374
                     /note="ORF_13; similar to Glycosyl transferase group 4;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr4"
                     /product="glycosyltransferase"
                     /protein_id="AAM27620.1"
                     /db_xref="GI:20559848"
                     /translation="MMNLWLLLPAVAGLSLLLTAGLRRYAIARSLMDVPNARSSHQVP
                     TPRGGGVAIVLSFLLAVLLAAFLGMMDMRLAIGILGAGVGTALLGFLDDHGHIAARWR
                     LLGHFAGACWLLYWLGGLPALVFFGFMVDLGWVGHIGAVFYLVWMLNLYNFMDGIDGI
                     ASVEAVCVCIGAVLLVVVSGVGSNNASQGIWLSALLASAVTGFLFWNFPPARIFMGDA
                     GSGFLGIILAGLSLQAAWVSSQLFWGWLILLGVFVVDATLTLLRRLLRGDKVYEAHRS
                     HAYQYASRYYGRHLPVTLAVGCINLVWLLPLALFVASGRIDGMLALLIGYLPLVALAL
                     RFKAGTLEPRVA"
     CDS             12559..>13401
                     /note="ORF_14; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /gene="wbpM"
                     /transl_table=11
                     /protein_id="AAM27621.1"
                     /db_xref="GI:20559849"
                     /translation="MLDNLRVRLLGLPRRQKRILQVATDIALVWLSLWLAFLVRLGTE
                     DMISPFGGHAWLFIAAPLVAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSL
                     LVYWYRSPPAVVPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDG
                     LPRVAIYGAGAAGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMI
                     DETGAQEVLLAIPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDI
                     ADLLG"
BASE COUNT     2848 a   2878 c   3721 g   3954 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgca agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga cctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 aaaaaagggc ggcctaggcc gccctttttc gttttccctt tcttggacct gttcaaagac
      181 tgatcagcat gctaaaagag acctgagctg atctagccgc ttgaaaaaga agggaaaacc
      241 atgaccaagt cggagttgat cgaacggatc gttacccatc aggggcaact gtccgcgaag
      301 gatgtcgagt tggcaatcaa gaccatgctg gagcaaatgt cccaggccct ggcgaccggg
      361 gaccggatcg agatccgtgg tttcggcagc ttttccttgc actaccgcgc cccgcgcgtc
      421 ggtcgcaacc ccaagaccgg ggagtcggta cgcctcgacg gcaagttcgt gccgcacttc
      481 aagccgggca aggagttgcg ggatcgggtc aacgagccgg agtgatcttg gcggcgatta
      541 agagggagtt ttccatgcga tggttcagac ggattttgac tgcggtggtt gtactgatgg
      601 ttgcgctgac ggtcatcatt tttgtcttgg aaaatcaaag tggtgtcaat ctatctttcc
      661 ttgggtggcg cacgcctgag gggccattat ccttgtttgt atctttagcc tttgttctcg
      721 gtggtttagg tggagtactc agcggcatgt ggctccgttt gataggttcc gtatccaaag
      781 gccatctgcc agttcgtcgt gaagctatga aatagctcta ctcctctgcg tttcagttga
      841 tgatatccct tgctattttc acttcgttga gcgagggcgt ttctctatcc actgccggca
      901 tgctgttgac ggtgggaaag gaagaggggc ggcaactaat ttctagctag aatgtctaaa
      961 agggctgcgg tataccctgc ttaggagggt gtgccatgca gttgttgtcg gcatccagtc
     1021 gctagtgctg attgccgacc cagatcggta gcagcggtgt aactgcttgt aatcttggag
     1081 catgctgtca aaatgcagca gcttcagccc atagctttgc tcatgctgct ggtgatttcc
     1141 gccaaccaat caaagacgga ctagttgtga cagaagaaac ctttttccag cgcgtggcca
     1201 aaaatgaaga gttcgatctg atagggcttt tacgaggctt gtgggctgaa aagtggctga
     1261 tagcttgtat aacgatattg actacatcct tggtcgggac atatgccttt ttaagtaagc
     1321 cggtttatga ggcacgtata gcggtactcc ctccatcatg gagcagtatt gcaggattta
     1381 atttggggcg agccgagaat ggattaacca cattctccgt taatgatgtt tattccgttt
     1441 ttttgcgaaa tcttttagct gaagagactc ggagaaattt ttttcgcgaa gtttatctgc
     1501 cttccttgag tgaaaacgag cgctctggtt cgaaagatcg tctttattcg aatctttctg
     1561 aagaaatgga aatacagaga cctagtaagg atgagcgtga gcgctacacc gtgatctttg
     1621 aaaagcacga tcccggtcag gcggctgact gggggcgtag atatataaag ctggctgccg
     1681 aaggctccat gaaggaaatg caagacgccg tgcagcgaga gtttgaggta aaaagtcgga
     1741 atattcagca acagatagat attttgcgcg atgcggcgaa ggcgcgtcgt gaagaccgca
     1801 tcaatcgttt gaaagaagct tatcaaatag ccgagtcgct gaagttggaa aatccccctt
     1861 tgattggcgg gcagatggat cagcagcttt cttctatcat ggagggagca ttgatgtata
     1921 tgagaggtac caaggccttg cgggcagaaa tcagggcttt ggaagagcga acgtcggacg
     1981 atccattcat tcctgctttg cgaaatttgc aagaaaagca agctatgtat tctactttga
     2041 aattggacgt aaataaggtc gcggtattca ggcaggatgg agtagccgaa gtgccggatg
     2101 atccagttcg cccgaagaag ggaatacttc tagtgctggg tgcgctctct ggactcgttt
     2161 ggggagtgat gattgctttc atcgttatgg ccgttagaag gtctcgcgca aaatagtcta
     2221 tatatttact gctatttgga tttttggata ttcaagagag ctttacaatt ctggtttggc
     2281 tttatagttg gaggttcctt tcatggatat taaggccgct gttgtcgggt tggggtatgt
     2341 tgggctacct ctggctgtgg agtttggaaa gaagcgatcc gtcacaggtt tcgatataaa
     2401 tcatagtcgt attcgagatc tgcagaaagg gtatgattct actcttgagg tcgaaaagga
     2461 agagctggaa agtgctgtac atctgaaatt tacttccact ctgagtgagc ttcaagagtg
     2521 taatttcttt attgttactg tgcctacacc cattgatgaa cataaacagc ctgatttgac
     2581 gcctctggtg aaggcatctg agagtatcgc caaggttctg aaaaagaatg acatcgtggt
     2641 gtatgagtcc acggtctatc ctggtgcgac tgaggaagtg tgcgtgcccg ttctggaaag
     2701 ggaatccggg ctaagattta attctgattt ttttgtaggc tatagtcctg aacgtataaa
     2761 cccgggggac aaagagcatc gtgtctcgac aatcaagaaa gtaacttcag ggtctacccc
     2821 tgaagtagca gagatagtcg attctcttta tagggaaata attacggctg ggactcataa
     2881 ggctgagagc ataaaagtag cagaagctgc caaggttatt gaaaatactc aacgtgatct
     2941 aaatattgcg cttataaatg agctggcaat tattttcaat aaacttgaga ttgataccga
     3001 gtccgtgctg caggcggcag gaacgaagtg gaatttcctc ccctttaggc cgggtcttgt
     3061 tggtgggcac tgtattggtg tagatcctta ttaccttacc cataaggctc aaagcatagg
     3121 ttatcatccg gaaatcatac tggcagggcg acgtctcaat gatggcatgg gtgcgtatgt
     3181 agtttctcag ctggtcaaag ccatgctaag aaagcgtatt catgtagacg gcgctcgtgt
     3241 tttggtcatg gggttgacct tcaaagagaa ctgtcccgat ttacgaaata caaaggtcgt
     3301 ggatatagtt cgggagttgg ccgaatacaa tatcgccgtg gatgtatatg atccttgggt
     3361 aagtgttgaa gaggctcagc atgagtatgg gcttactcct atttccgcgc ccgtcgaggg
     3421 caactatgat gcggttgtcc tagcggtagc tcataacgag ttcaaggagc ttggtgcgga
     3481 taagatcagg gcattcggga aacttgaaag tgtaatatat gatttgaagt ttatcttgga
     3541 taagtcagat tctgacttgc gcttgtaagg aggggtcatg aacgcttacg ctaaggcttt
     3601 aagtaggcta gagactacta ctagtagttg gctggtcact ggggttgccg gttttattgg
     3661 atcgaatctt ctggagttac ttctgaaatt taatcagaag gttgtggggt tggatgattt
     3721 ttctaccggg catatgtata accttgatga agtgaggggg cttgttggcg aggagagatg
     3781 gaggaacttc aagcttatac agggggatat cagagacatt ggtgattgca ggcgtgcagt
     3841 cgagggcgtc gattatgttc ttcatcaggc cgccttgggt tccgttcctc gctcaattgc
     3901 agatccgatt actacaaact cggtaaatat ctcaggattt ctgaatatcc tcgtggcgtc
     3961 caaagaggcg ggggttaaga gccttaccta tgcagctagt agttcaacct atggtgacca
     4021 cccaggattg cctaaggtag aggaagttat agggaaacca ctgtcgccct atgcagtaac
     4081 caagtacgtc aatgagcttt atgctgatgt cttttcgcgc tgctatggct tcaacttgat
     4141 tggtttgaga tattttaatg tttttggaaa acggcaggat cctaatgggg cctatgctgc
     4201 agtgattcca aaatggattt cagctatgat aaagggtgag cccgtatata ttaatgggga
     4261 tggtgaaact agtcgggatt tttgcttcat taataatgtg ataaaagcga atctgctggc
     4321 tgctttttgt gaaaccgaag aggcaaaaaa tcaggtttat aatgttgctt atggtggtcg
     4381 cacaacattg ggtcagcttt ttgactcgct aaaagttgag ctttcccgga acggtattaa
     4441 ctatgagcaa ggacctattt atagggagtt tcgtgcgggg gatgtacgtc actctcaagc
     4501 ggaaatcaaa aaggctcaag cctatctagg ctataatccc gaatatgata tccaggctgg
     4561 tattgctgag gctatgcctt ggtatatatc ttttgtaaag taggctcttg gaagatgaag
     4621 ctctattcaa gcagtttcct gaatagctct gagagtaagc ttgcatgaat ccaaaaaata
     4681 tattttggaa tctgctggga ttagggcttc ctttaattat tgccgctctg acagtgccac
     4741 atctcattgc cactgtagga gttgaaaggt ttggctttct tgctctgtca tgggggctta
     4801 ttggctatgc cggaatactt gatctgggga taggtcgagc tgttactcag aaactctcct
     4861 ctattcgtgg ctctgatgag gagatgaatt catacacaat aatgagtaca gctattcgga
     4921 tcaccgccat tgttggtggg ctgggctttt ccctgattat catatttggc ctcctggggg
     4981 gcggagaatt tttttcccat aaagatgtca gttcgcttga gctagaattc tctgttttac
     5041 tattggcttt tgccttacct ttgcaagcta taagtgctac ctatagaggg gtgaatgagg
     5101 cgtatctcaa cttcaaaggg atcaacctct tacgtatctt tcttggggcg actaattttg
     5161 gtggtccttt tctagtttct ttctatgcaa aggatcttca ttatcttgta actacccttg
     5221 tcctgtctcg ggctttggct ttctttatat tccggcgttt agctcatgga gtactcaaag
     5281 aaacactggt ggagagaagt tgcaaatatg atcggcgtca ggctgttgag ttgttccgat
     5341 ttggtggttg ggtcactgta agtagtataa taaatccttt tcttgttcag tccgataggt
     5401 tttttattgg tgttttattg tctgctgcag cggtaacctc ttatgtaatt ccatatgaaa
     5461 tcactattca gtcgatgatc cttgtgggtg ctgtttccac cgttgccttt ccgtctatat
     5521 caaatttaat tcgcacttcc tttgctgaag ctctggagtg ttttaataaa tggctcttgc
     5581 gtgtgctgtt gataatggga ggagggatgc tttgcctggc ttttcttttg ccctttattt
     5641 tgaagttgtg ggtgggggat tatatagggg acgactctat cagtgtagga aggatccttt
     5701 gtcttggagt gtttttcaat gcgttgggtg ctatgttcta ttctttcttg catgccaacg
     5761 ccaaggtgaa agaaactgca attttgcact ccatagagct accaatattt atcctcattt
     5821 tgattattct gattccacga catgggatcg tgggggcggc tgttgcgtgg tcgctgagaa
     5881 cattagtgga taccatagtg cttgctgttt tgagctattt ttcaggctgg agagtgaaat
     5941 ttgctaactg ctagtgcaag agtcgccagc ttttttattg ctactcttct gattttttct
     6001 ttggtttttc ctacggcttt gatcggtatt aaattcattt tactgatctt ggcattattg
     6061 tttacgctta tctctagcct cgctcgggct gatctgaacc tatatgtact attggctgga
     6121 ttgttttata gcggtgtagg gttggtttgg tctatttatg gtgagttgag ggggaatgca
     6181 ggggctctac aggtacttac cgtcatggtg gtatatcctg ttctcttcgt tttgttggca
     6241 gtttcttata gaggacaatc tgaggcgcta ggtaagttgt ttttttacgc ggcacttttg
     6301 atagctgttc ttgatatatt tttcgttttt ggtaattacc ttttttccta caatccagtt
     6361 tatgagtcca tggctgcgat gtatggcgat gctgctgtgg tggataatgc gggaagatat
     6421 ataaagttta cgcttcccaa tgtttcatct ttgatattct tgatccccta tgtggtcagt
     6481 tatcttctgt gccgaaaatt ctctctttcc ggtctatttc ttctggccct tttgctactg
     6541 gtagcatttg tttcaggcag gagagcactg ttgcttgttg cttttacagc acctttcttg
     6601 gcttattttc ttaccatggg tgccgaccgt tttcaatgga aagtcatttc tcgtgtttta
     6661 ctggttttta tagtcttcat gttgggtttt ttgatttttt ataatacgta tcctgagtat
     6721 gtcgaaaacc tttttttatc cataagtaat ttttcagata atgatagcaa tcttgagcga
     6781 cggtatcagt tcgaagcctt gatgaaggga gtctatcagc aaccattctt cggaatgggc
     6841 gcaggagcta ttgctgatta tattcgttcg gaagaaatgc cgtgggcgta tgaacttttc
     6901 tatgtttcac ttgtttttca atatgggatt ctgggtttct tgttctatgc tttgggcgtg
     6961 ctttttttgc ttgtctttct agttttgcgt gtgagaaaaa ttggaaggag aagctttgag
     7021 ttttgctatc tgtccggcat gttagcattc ctaatggcta cggcaaccaa tccatatttg
     7081 gcaaaatttg attatatgtg ggtaatattt attccggtag cattgcttaa ttacagtgcc
     7141 gtttctaggg ggaaggatgg aaagtccaat tattgatgtt gtcgttgtta actggaatgc
     7201 gggccttcag ttgcgtgcct gtgttgactc catttttaaa aactcttccg gactgataag
     7261 aaaagtcatt gtgatcgata atggctctaa cgataactct tgtgcccttc ttggtgaacg
     7321 ggagaatctt gaggtcgtgt tgacaggaga gaatctcggt ttcgctacag cctgcaatct
     7381 cggagcttcc aaaggaaagg ctgaatatat acttttcctg aatccagatg cggaactcta
     7441 tgaagagacg ataggttctg ttgttcgctt catgaataga tccgaaaact ctaaggtagg
     7501 agtttgtgga gtgcaattaa tcgatgaaca aggacaggtt agtcgtagct gtgctcgatt
     7561 tccctcttcc gcaagaatgt tgatgcatac tatgggcgtt gatcgaatct ttcctgctct
     7621 cggatatgca atgactgatt gggatcactt gtcatccagg cctgtagatc atgttatcgg
     7681 agcgttctat tttgtaaggc ggcatgtttt caagctggtc ggtggctttg atgagcgctt
     7741 cttcgtctat ctagaggacc ttgatctgtc ttaccgtata tcctgttctg gatggagtag
     7801 tttttacctt tccactgccc aggcattcca tgccggtgga ggtgcctcaa ggcagatcaa
     7861 ggcgcggcgg cttttctact ccctgagaag ccgacttatc tatattttca agaaccttcc
     7921 gaccgtttct gcgtttttga ttctgttgtc cagcttgttt tttgagcctg tcattagatc
     7981 tatgttttcc atggttaaac tttcctttcc tgctcttcgg gaaacctggt cagcttattt
     8041 aatgctttac cgctggttgc cgaagtggat tttctttaac gctacgcgat agctattatg
     8101 aaaatattgt tgctttccaa gtacccacgc ctaggagcca gcagccgact gcggactctc
     8161 caatatatcg attatctaga gtcgaaagga ggaagggtag atgttagaag tttttttgat
     8221 gatggctacc tgtctggtct ctattcctcg gggcgtcgtt ctttttataa tgttttaaag
     8281 tcatacatct cgcgtttctt tgtacttttc actgtctgta ggtacgattt aatctggatt
     8341 gaaaaagaga tttttccgta ttttccttct gtggctgaga ggtttttaag attttttgga
     8401 aagccttatg tggttgacta cgatgatgcc atttttcata actatgatct ttctgggaat
     8461 tggttggtca gaaaatttct cggcaaaaaa attgatagcg tcatgcgtca ctcttcatgt
     8521 gtgatcgcag gtaatgacta cttagctgag cgagcccggg ttgctggttc atctcgagtg
     8581 gaaattattc ctacagtgat cgatctcgaa cgttacgagg tcagagatga gtcttgttct
     8641 aagaaaatag tcataggctg gattggttct ccttcgactc agaagtatct actcgatatt
     8701 gcccctgaac tagtgaagct ctgtgtcgaa tatcaggcat ccatgctcct tgtaggggct
     8761 acgcccgaga tatcatccta cttctcaggc gtctatgtgg atgtcaaagc gtggaaggaa
     8821 gaagatgagg taggatatat tcggcagatg acaattggta taatgccgtt gcccgatggc
     8881 ccttgggaaa aaggaaagtg tggatataag ctaattcaat atatggcatg tggcgttccc
     8941 gtcgttgcct cgccagttgg tgttaatatt gaaatcgtcg aaaataatga gtcaggactt
     9001 ctggctgagg gaaatagagg atggcgtgaa tccttgcaga agcttctgca gtcttccgaa
     9061 ctcaggcggg agtgcgggcg gaacggacgt tcctcggtag aaaaaatata ttcccttcaa
     9121 gttcagtccc gccgtcttca tcagacattt actgaagttt tgagttagtc gccggtctat
     9181 tgagtctgct tgctggggat ttttgggtga agttaggtat gagtgctaaa agctgtcagg
     9241 agcctgtatg aggccggttt tgctaatggt gataaacgat ccggcgttct ttatatctca
     9301 tcgtctaccc attgcgacag gggctaggag ttccggtttc gatgtgcata ttgcgaccag
     9361 acccggacag gctgtatcaa ggctcatagc ggaaggtttt cggcatcatg aactgccttt
     9421 gtccagaagt ggaaaaaatc ctttttcgga gttttatctc ttgctgtcta tatggcgtct
     9481 gctgtggaag ctacgccccg atattttgca tctggtcacg ataaaaccgg tcttatatgg
     9541 cggtatcgct gcccgtctgg cgccggtgaa gggagttgta gcggctgttt ctggacttgg
     9601 tttcgtcttt atggccaagg gctcgaaggc cagtgtgttt cgctcaggga tcgcttggct
     9661 ttatcggcgg gccttgggca agaaaaggtt gcgtgtcatc tttcagaatc ccgatgatcg
     9721 ggatgcgctg attggcttgg gggcgatcac ttatgaaaag tccgtcctga tccgcggctc
     9781 tggagttgac ctgtcgctgt gccatccggc accggaaagt cctggtacgc cggtggtcac
     9841 tctggcagct cgcttgctca gagataaagg tgtagtggag ttcgtcgaag cggcaaatat
     9901 cctcaggcag cgtggtgtgt cagcgcattt ccagttggtc ggggatttgg atccggggaa
     9961 tcccagcagt atcgaagagt tcgacctgga gcggtggcgg agcgaaggta cggtcgagtg
    10021 tcttggttat cgtcaggata ttgcgtccgt cttcgctcat tcgcatatag ttgtcttgcc
    10081 ttcgtatcgc gaaggacttc ccaaggtcct ggttgaggct gctgcctgcg gtcgcgtagt
    10141 cgtgacgaca gatgtgccgg gttgccggga cgctatcgaa gcggatagaa ccggcctgct
    10201 ggtgccagtt cgtgacgcag tggcgttggc tgacgccatt cagcgtcttg tcgaatctcc
    10261 ggagctgcgg aagaagatgg gggctgctgg gcggacattg gcagagcgtg attttgcgat
    10321 cgaaagtatc gtccaacagc acctcgatat ctatcgggca ctagggagcg gagcttgagt
    10381 cgacacaatg tattggtgac gggcgctacc ggattcgtcg gggctgcgtt ggtcgagagt
    10441 ttttggtctt cggaaaagta cagggtctgg gccggttacc gtcgagagag tagcccctgg
    10501 cccgaggggg ttaccccttt gttcttgggg gagctgggta gttccgtggc ctgggagacg
    10561 gaatcgctca tcgacacggt gatccattgt gccgcgcgtg tgcatgtcat gagcgagacg
    10621 gtttccgatc cgcttgccga gtttcgcaag gccaatgtcc agggtaccct cgatctggcc
    10681 cgtcaggccg catccctggg agttcgtcgt tttatctatc tcagctcgat caaagtcaat
    10741 ggcgagggga gcgagcccgg gcagccctac ttcgccgata gcctgcctaa tccagtcgat
    10801 ccctatggtg tttccaagct ggaggcggag cgggcgctcc tggacctggc ggcggagacc
    10861 ggcctcgagg ttgtgatcat tcgtcctgtc ctggtgtatg gccctggggt caaggccaac
    10921 gtacagacta tgatgcgctg gctgaagaga ggggttccgc ttcccttggg ggcgatacac
    10981 aatcggcgca gtctggtggc gctggataac ttgaccgatt tgatcgctac ctgtatcgat
    11041 cacccggcag cggttaacca ggtctttctg gtaagcgacg gggaggatct ttccactact
    11101 gacctgcttc ggagaatggg gaacgcgtta cgtacgcccg cgcgcctggt gcctgttccc
    11161 gccagttgga ttggcctggt cgccaagacg ctgaacaagc aggcgtttgc tcagcgcctg
    11221 tgcggttcgc tgcaggtcga tatagaaaaa aaccgtcgga tgcttggctg gacgccgcct
    11281 gtaggcgtgg acgaggcttt ggaaaaaacc gcccgctatt tcctggatca ccaatgatga
    11341 atctgtggtt gttgttgccg gcggttgccg gcctttccct cttgctgacc gcggggctgc
    11401 gtcgttatgc gattgctcgc agtctgatgg acgtacccaa tgcccgaagt tcccaccagg
    11461 ttccgacacc acgaggcggc ggtgtcgcta tcgtgctttc cttcctgctg gcagtcctgt
    11521 tggcggcctt cctgggcatg atggatatga ggctggccat cggcatcctc ggcgcaggcg
    11581 tggggaccgc attgctgggt tttctggatg accacggtca tatcgctgcc cgttggcgtc
    11641 tgctcgggca tttcgccggc gcctgctggc tgctgtactg gctgggaggc ctgccggcgc
    11701 tggtcttttt cggttttatg gtcgacctag ggtgggtcgg acatataggt gcggtgttct
    11761 acttggtctg gatgctcaat ctctacaatt tcatggatgg tatcgatggg attgccagcg
    11821 tcgaggctgt gtgcgtgtgc attggtgctg ttctgcttgt cgtcgtcagt ggcgtcggtt
    11881 ccaataatgc atcccaaggc atctggttgt cggcactgct ggcgtccgcg gtgacgggtt
    11941 tcctcttctg gaacttcccc ccggcgcgga tcttcatggg agacgccggt agcggttttt
    12001 tgggaatcat ccttgcaggc ctttccctgc aggctgcctg ggtctcgtcg caactcttct
    12061 ggggatggct gatcctgctg ggggttttcg tcgtcgatgc cactctgacc ctgctgcgtc
    12121 gattgctgcg gggagacaag gtatatgagg cacatcgcag tcatgcatat cagtacgcct
    12181 ctcgttatta cggacgtcac ctgcctgtca ccctggcagt tgggtgtatc aatcttgtct
    12241 ggttgctacc attggcgcta tttgttgcgt cgggtcgaat tgacggcatg cttgcgttgt
    12301 tgatcggcta cctgcctttg gtagccctgg cccttcggtt caaggccgga accctggaac
    12361 cacgcgttgc ctgaatgccc cttcggctag aggagcgggc tggttctgca gaaacgtata
    12421 ttatgaaggg aatgtgcagg cgattatcta gtgtgtgaat accgctaata tcgcgacttg
    12481 gattccagaa tggcatgtgc cagatcgaat aaatggattg gccgggtacg gttgtattga
    12541 agggggctga ttaataggat gttggataac ttgcgtgtac gcctcttggg attacctcgc
    12601 cgccaaaagc ggattctcca ggtcgcgacg gacatcgctc tggtatggct ctcattgtgg
    12661 ctggcctttc tggtgcgtct tggcacggaa gacatgatca gccccttcgg cggccatgcc
    12721 tggctgttca tcgccgcccc gttggtggcc attcccctgt tcatccgctt cggcatgtac
    12781 cgggcggtga tgcgctacct gggcaacgac gcccttatcg cgatcgccaa ggccgtcacc
    12841 atttccgctc tggtcctgtc gttgctggtc tattggtacc gctccccgcc ggcggtggtg
    12901 ccgcgttccc tggtgttcaa ctactggtgg ctgagcatgc tgctgatcgg cggcttgcgt
    12961 ctggccatgc gccagtattt catgggcgac tggtactctg ctgtgcagtc ggtaccattt
    13021 ctcaatcgcc aggacggcct gcccagggtg gctatctatg gcgcgggggc ggccggcaac
    13081 cagttggttg cggcattgcg tctcggtcgg gcgatgcgtc cggtggcgtt catcgatgat
    13141 gacaagcaga tcgccaaccg ggtcatcgcc ggtctgcggg tctataccgc caagcatatc
    13201 cgccagatga tcgacgagac gggcgcgcag gaggttctcc tggcgattcc ttccgccact
    13261 cgggcccggc gccgagagat tctcgagtcc ctggagccgt tcccgctgca cgtgcgcagc
    13321 atgcccggct tcatggacct ggccagcggc cgggtcaagg tggacgacct gcaggaggtg
    13381 gacatcgctg acctgctggg g
//
