LOCUS       AF498405               13413 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 014 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498405 AC104724
VERSION     AF498405.1  GI:20559850
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 13413)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975253.
FEATURES             Location/Qualifiers
     source          1..13413
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="014"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27622.1"
                     /db_xref="GI:20559851"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             241..525
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27623.1"
                     /db_xref="GI:20559852"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEPE"
     CDS             597..815
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27624.1"
                     /db_xref="GI:20559853"
                     /translation="MVALTVIIFVLENQSGVNLSFLGWRTPEGPLSLFVSLAFVLGGL
                     GGVLSGMWLRLIGSVSKGHLPVRREAMK"
     CDS             1167..2216
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27625.1"
                     /db_xref="GI:20559854"
                     /translation="MTEETFFQRVAKNEEFDLIGLLRGLWAEKWLIACITILTTSLVG
                     TYAFLSKPVYEARIAVLPPSWSSIAGFNLGRAENGLTTFSVNDVYSVFLRNLLAEETR
                     RNFFREVYLPSLSENERSGSKDRLYSNLSEEMEIQRPSKDERERYTVIFEKHDPGQAA
                     DWGRRYIKLAAEGSMKEMQDAVQREFEVKSRNIQQQIDILRDAAKARREDRINRLKEA
                     YQIAESLKLENPPLIGGQMDQQLSSIMEGALMYMRGTKALRAEIRALEERTSDDPFIP
                     ALRNLQEKQAMYSTLKLDVNKVAVFRQDGVAEVPDDPVRPKKGILLVLGALSGLVWGV
                     MIAFIVMAVRRSRAK"
     CDS             2303..3568
                     /note="ORF_5; similar to UDP-glucose/GDP-mannose
                     dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpA"
                     /protein_id="AAM27626.1"
                     /db_xref="GI:20559855"
                     /translation="MDIKAAVVGLGYVGLPLAVEFGKKRSVTGFDINHSRIRDLQKGY
                     DSTLEVEKEELESAVHLKFTSTLSELQECNFFIVTVPTPIDEHKQPDLTPLVKASESI
                     AKVLKKNDIVVYESTVYPGATEEVCVPVLERESGLRFNSDFFVGYSPERINPGDKEHR
                     VSTIKKVTSGSTPEVAEIVDSLYREIITAGTHKAESIKVAEAAKVIENTQRDLNIALI
                     NELAIIFNKLEIDTESVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQSIGYHP
                     EIILAGRRLNDGMGAYVVSQLVKAMLRKRIHVDGARVLVMGLTFKENCPDLRNTKVVD
                     IVRELAEYNIAVDVYDPWVSVEEAQHEYGLTPISAPVEGNYDAVVLAVAHNEFKELGA
                     DKIRAFGKLESVIYDLKFILDKSDSDLRL"
     CDS             3578..4603
                     /note="ORF_6; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /protein_id="AAM27627.1"
                     /db_xref="GI:20559856"
                     /translation="MNAYAKALSRLETTTSSWLVTGVAGFIGSNLLELLLKFNQKVVG
                     LDDFSTGHMYNLDEVRGLVGEERWRNFKLIQGDIRDIGDCRRAVEGVDYVLHQAALGS
                     VPRSIADPITTNSVNISGFLNILVASKEAGVKSLTYAASSSTYGDHPGLPKVEEVIGK
                     PLSPYAVTKYVNELYADVFSRCYGFNLIGLRYFNVFGKRQDPNGAYAAVIPKWISAMI
                     KGEPVYINGDGETSRDFCFINNVIKANLLAAFCETEEAKNQVYNVAYGGRTTLGQLFD
                     SLKVELSRNGINYEQGPIYREFRAGDVRHSQAEIKKAQAYLGYNPEYDIQAGIAEAMP
                     WYISFVK"
     CDS             4665..5954
                     /note="ORF_7; similar to Polysaccharide biosynthesis
                     protein; potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AAM27628.1"
                     /db_xref="GI:20559857"
                     /translation="MNPKNIFWNLLGLGLPLIIAALTVPHLIATVGVERFGFLALSWG
                     LIGYAGILDLGIGRAVTQKLSSIRGSDEEMNSYTIMSTAIRITAIVGGLGFSLIIIFG
                     LLGGGEFFSHKDVSSLELEFSVLLLAFALPLQAISATYRGVNEAYLNFKGINLLRIFL
                     GATNFGGPFLVSFYAKDLHYLVTTLVLSRALAFFIFRRLAHGVLKETLVERSCKYDRR
                     QAVELFRFGGWVTVSSIINPFLVQSDRFFIGVLLSAAAVTSYVIPYEITIQSMILVGA
                     VSTVAFPSISNLIRTSFAEALECFNKWLLRVLLIMGGGMLCLAFLLPFILKLWVGDYI
                     GDDSISVGRILCLGVFFNALGAMFYSFLHANAKVKETAILHSIELPIFILILIILIPR
                     HGIVGAAVAWSLRTLADTIVLAVLSYFSGWRVKFANC"
     CDS             6001..7175
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy(pseudo)"
                     /product="polymerase"
     CDS             7156..8091
                     /note="ORF_9; similar to Glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27629.1"
                     /gene="gtr1"
                     /product="glycosyltransferase"
                     /db_xref="GI:20559858"
                     /translation="MKSPIIDVVVVNWNAGLQLRSCVDSIFKNSFGLIRKVIVIDNGS
                     TDNSCALLGERENLEVVLTGENLGFATACNLGASKGEAEYILFLNPDAELYEETIGSV
                     VRFMNRSENSKVGVCGVQLIDEQGQVSRSCARFPSSARMLMHTMGVDRVFPALGYAMT
                     DWDHLSSRPVDHVIGAFYFVRRHVFKLVGGFDERFFVYLEDLDLSYRISCSGWSSFYL
                     STAQAFHAGGGASRQIKARRLFYSLRSRLIYIFKNLPVVSAFLILLSSLFFEPVIRSM
                     FSMVKLSLPALRETWSAYLMLYRWLPKWIFFNATR"
     CDS             8097..9167
                     /note="ORF_10; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27630.1"
                     /gene="gtr2"
                     /product="glycosyltransferase"
                     /db_xref="GI:20559859"
                     /translation="MKILLLSKYPRLGASSRLRTLQYIDYLESKGGRVDVRSFFDDGY
                     LSGLYSSGRRSFCNVLKSYISRFFVLFTVYRYDLIWIEKEIFPYFPSVAERFLRFFGK
                     PYVVDYDDAIFHNYDLSRNWLVRKFLGKKIDSVMRHSSCVIAGNDYLAERARVAGSSR
                     VEIIPTVIDLKRYEVRDESCFKKTVIGWIGSPSTQKYLLDIAPELVKLCVEYQASMLL
                     VGATPEISSYFSGVYVDVKAWKEEDEVGYIRQMTIGIMPLPDGPWEKGKCGYKLIQYM
                     ACGVPVVASPVGVNIEIVENNESGLLAEGSRGWRESLQKLLQSSELRRECGRNGRSSV
                     EKIYSLQVQSRRLHQTFIEVLS"
     CDS             9610..10377
                     /note="ORF_11; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27631.1"
                     /gene="gtr3"
                     /product="glycosyltransferase"
                     /db_xref="GI:20559860"
                     /translation="MAKGSKASVFRSGIAWLYRRALGKKRLRVIFQNPDDRDALIGLG
                     AITFEKSVLIRGSGVDLSLCHPVPESPGAPVVTLAARLLRDKGVVEFVEAANILRQRG
                     VSAHFQLVGDLDPGNPSSIEEFDLERWRSEGTVECLGYRQDIASVFAHSHIVVLPSYR
                     EGLPKVLVEAAACGRVVVTTDVPGCRDAIEADRTGLLVPVRDAVALADAIQRLVESPE
                     LRKKMGAAGRTLAERDFAIESIVQQHLDIYRALGSGA"
     CDS             10395..11336
                     /note="ORF_12; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /protein_id="AAM27632.1"
                     /db_xref="GI:20559861"
                     /translation="MTGATGFVGAALVESLCSSEKYRVWAGYRRESSPWPEGVTPLFL
                     GELGSSVAWETESVIDTVVHCAARVHVMSETVSDPLAEFRKANVLGTLDLARQATSLG
                     VRRFIYLSSIKVNGEGGEPGQPYVADSLPNPVDPYGVSKLEAEQALLGLAAETGLEVV
                     IIRPVLVYGPGVKANVQTMMRWLKRGVPLPLGAIHNRRSLVALDNLTDLIATCIDHPA
                     AVNQVFLVSDGEDLSTTDLLRRMGNALRTPARLVPVPASWIGLVAKTLNKQAFAQRLC
                     GSLQVDIEKNRRMLGWTPPVGVDEALEKTARYFLDHQ"
     CDS             11333..12373
                     /note="ORF_13; similar to Glycosyl transferase group 4;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr4"
                     /product="glycosyltransferase"
                     /protein_id="AAM27633.1"
                     /db_xref="GI:20559862"
                     /translation="MMSLWLLLPAVAGLSLLLTAGLRRYAIARSLMDVPNARSSHQVP
                     TPRGGGVAIVLSFLLAVLLAAFLGMMDISLAIGILGAGVGTALLGFLDDHGHIAARWR
                     LLGHFAGACWLLYWLGGLPALVFFGFMIDLGWVGHIGAVFYLVWMLNLYNFMDGIDGI
                     ASVEAVCVCIGAVLLVVVSGVGSDDASQGIWLSALLASAVTGFLFWNFPPARIFMGDA
                     GSGFLGIILAGLSLQAAWVSPQLFWGWLILLGVFVVDATLTLLRRLLRGDKVYEAHRS
                     HAYQYASRYYGRHLPVTLAVGCINLVWLLPLALFVASGRIDGMLALLIGYLPLVALAL
                     RFKAGTLEPRVA"
     CDS             12571..>13413
                     /note="ORF_14; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpM"
                     /protein_id="AAM27634.1"
                     /db_xref="GI:20559863"
                     /translation="MLDNLRVRLLGLPRRQKRILQVATDIALVWLSLWLAFLVRLGTE
                     DMISPFGGHAWLFIAAPLVAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSL
                     LVYWYRSPPAVVPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDG
                     LPRVAIYGAGAAGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMI
                     DETGAQEVLLAIPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDI
                     ADLLG"
BASE COUNT     2835 a   2899 c   3737 g   3942 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgta agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga cctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 aaaaaagggc ggcctaggcc gccctttttc gttttccctt tcttggacct gttcaaagac
      181 tgatcagcat gctaaaagag acctgagctg atctagccgc ttgaaaaaga agggaaaacc
      241 atgaccaagt cggagttgat cgaacggatc gttacccatc aggggcaact gtccgcgaag
      301 gatgtcgagt tggcaatcaa gaccatgctg gagcaaatgt cccaggccct ggcgaccggg
      361 gaccggatcg agatccgtgg tttcggcagc ttttccttgc actaccgcgc cccgcgcgtc
      421 ggtcgcaacc ccaagaccgg ggagtcggta cgcctcgacg gcaagttcgt gccgcacttc
      481 aagccgggca aggagttgcg ggatcgggtc aacgagccgg agtgatcttg gcggcgatta
      541 agagggagtt ttccatgcga tggttcagac ggattttgac tgcggtggtt gtactgatgg
      601 ttgcgctgac ggtcatcatt tttgtcttgg aaaatcaaag tggtgtcaat ctatctttcc
      661 ttgggtggcg cacgcctgag gggccattat ccttgtttgt atctttagcc tttgttctcg
      721 gtggtttagg tggagtactc agcggcatgt ggctccgttt gataggttcc gtatccaaag
      781 gccatctgcc agttcgtcgt gaagctatga aatagctcta ctcctctgcg tttcagttga
      841 tgatatccct tgctattttc acttcgttga gcgagggcgt ttctctatcc actgccggca
      901 tgctgttgac ggtgggaaag gaagaggggc ggcaactaat ttctagctag aatgtctaaa
      961 agggctgcgg tataccctgc ttaggagggt gtgccatgca gttgttgtcg gcatccagtc
     1021 gctagtgctg attgccgacc cagatcggta gcagcggtgt aactgcttgt aatcttggag
     1081 catgctgtca aaatgcagca gcttcagccc atagctttgc tcatgctgct ggtgatttcc
     1141 gccaaccaat caaagacgga ctagttgtga cagaagaaac ctttttccag cgcgtggcca
     1201 aaaatgaaga gttcgatctg atagggcttt tacgaggctt gtgggctgaa aagtggctga
     1261 tagcttgtat aacgatattg actacatcct tggtcgggac atatgccttt ttaagtaagc
     1321 cggtttatga ggcacgtata gcggtactcc ctccatcatg gagcagtatt gcaggattta
     1381 atttggggcg agccgagaat ggattaacca cattctccgt taatgatgtt tattccgttt
     1441 ttttgcgaaa tcttttagct gaagagactc ggagaaattt ttttcgcgaa gtttatctgc
     1501 cttccttgag tgaaaacgag cgctctggtt cgaaagatcg tctttattcg aatctttctg
     1561 aagaaatgga aatacagaga cctagtaagg atgagcgtga gcgctacacc gtgatctttg
     1621 aaaagcacga tcccggtcag gcggctgact gggggcgtag atatataaag ctggctgccg
     1681 aaggctccat gaaggaaatg caagacgccg tgcagcgaga gtttgaggta aaaagtcgga
     1741 atattcagca acagatagat attttgcgcg atgcggcgaa ggcgcgtcgt gaagaccgca
     1801 tcaatcgttt gaaagaagct tatcaaatag ccgagtcgct gaagttggaa aatccccctt
     1861 tgattggcgg gcagatggat cagcagcttt cttctatcat ggagggagca ttgatgtata
     1921 tgagaggtac caaggccttg cgggcagaaa tcagggcttt ggaagagcga acgtcggacg
     1981 atccattcat tcctgctttg cgaaatttgc aagaaaagca agctatgtat tctactttga
     2041 aattggacgt aaataaggtc gcggtattca ggcaggatgg agtagccgaa gtgccggatg
     2101 atccagttcg cccgaagaag ggaatacttc tagtgctggg tgcgctctct ggactcgttt
     2161 ggggagtgat gattgctttc atcgttatgg ccgttagaag gtctcgcgca aaatagtcta
     2221 tatatttact gctatttgga tttttggata ttcaagagag ctttacaatt ctggtttggc
     2281 tttatagttg gaggttcctt tcatggatat taaggccgct gttgtcgggt tggggtatgt
     2341 tgggctacct ctggctgtgg agtttggaaa gaagcgatcc gtcacaggtt tcgatataaa
     2401 tcatagtcgt attcgagatc tgcagaaagg gtatgattct actcttgagg tcgaaaagga
     2461 agagctggaa agtgctgtac atctgaaatt tacttccact ctgagtgagc ttcaagagtg
     2521 taatttcttt attgttactg tgcctacacc cattgatgag cataaacagc ctgatttgac
     2581 gcctctggtg aaggcatctg agagtatcgc caaggttctg aaaaagaatg acatcgtggt
     2641 gtatgagtcc acggtctatc ctggtgcgac tgaggaagtg tgcgtgcccg ttctggaaag
     2701 ggaatccggg ctaagattta attctgattt ttttgtaggc tatagtcctg aacgtataaa
     2761 cccgggggac aaagagcatc gtgtctcgac aatcaagaaa gtaacttcag ggtctacccc
     2821 tgaagtagca gagatagtcg attctcttta tagggaaata attacggctg ggactcataa
     2881 ggctgagagc ataaaagtag cagaagctgc caaggttatt gaaaatactc aacgtgatct
     2941 aaatattgcg cttataaatg agctggcaat tattttcaat aaacttgaga ttgataccga
     3001 gtccgtgctg caggcggcag gaacgaagtg gaatttcctc ccctttaggc cgggtcttgt
     3061 tggtgggcac tgtattggtg tagatcctta ttaccttacc cataaggctc aaagcatagg
     3121 ttatcatccg gaaatcatac tggcagggcg acgtctcaat gatggcatgg gtgcgtatgt
     3181 agtttctcag ctggtcaaag ccatgctaag aaagcgtatt catgtagacg gcgctcgtgt
     3241 tttggtcatg gggttgacct tcaaagagaa ctgtcccgat ttacgaaata caaaggtcgt
     3301 ggatatagtt cgggagttgg ccgaatacaa tatcgccgtg gatgtatatg atccttgggt
     3361 aagtgttgaa gaggctcagc atgagtatgg gcttactcct atttccgcgc ccgtcgaggg
     3421 caactatgat gcggttgtcc tagcggtagc tcataacgag ttcaaggagc ttggtgcgga
     3481 taagatcagg gcattcggga aacttgaaag tgtaatatat gatttgaagt ttatcttgga
     3541 taagtcagat tctgacttgc gcttgtaagg aggggtcatg aacgcttacg ctaaggcttt
     3601 aagtaggcta gagactacta ctagtagttg gctggtcact ggggttgccg gttttattgg
     3661 atcgaatctt ctggagttac ttctgaaatt taatcagaag gttgtggggt tggatgattt
     3721 ttctaccggg catatgtata accttgatga agtgaggggg cttgttggcg aggagagatg
     3781 gaggaacttc aagcttatac agggggatat cagagacatt ggtgattgca ggcgtgcagt
     3841 cgagggcgtc gattatgttc ttcatcaggc cgccttgggt tccgttcctc gctcaattgc
     3901 agatccgatt actacaaact cggtaaatat ctcaggattt ctgaatatcc tcgtggcgtc
     3961 caaagaggcg ggggttaaga gccttaccta tgcagctagt agttcaacct atggtgacca
     4021 cccaggattg cctaaggtag aggaagttat agggaaacca ctgtcgccct atgcagtaac
     4081 caagtacgtc aatgagcttt atgctgatgt cttttcgcgc tgctatggct tcaacttgat
     4141 tggtttgaga tattttaatg tttttggaaa acggcaggat cctaatgggg cctatgctgc
     4201 agtgattcca aaatggattt cagctatgat aaagggtgag cccgtatata ttaatgggga
     4261 tggtgaaact agtcgggatt tttgcttcat taataatgtg ataaaagcga atctgctggc
     4321 tgctttttgt gaaaccgaag aggcaaaaaa tcaggtttat aatgttgctt atggtggtcg
     4381 cacaacattg ggtcagcttt ttgactcgct aaaagttgag ctttcccgga acggtattaa
     4441 ctatgagcaa ggacctattt atagggagtt tcgtgcgggg gatgtacgtc actctcaagc
     4501 ggaaatcaaa aaggctcaag cctatctagg ctataatccc gaatatgata tccaggctgg
     4561 tattgctgag gctatgcctt ggtatatatc ttttgtaaag taggctcttg gaagatgaag
     4621 ctctattcaa gcagtttcct gaatagctct gagagtaagc ttgcatgaat ccaaaaaata
     4681 tattttggaa tctgctggga ttagggcttc ctttaattat tgccgctctg acagtgccac
     4741 atctcattgc cactgtagga gttgaaaggt ttggctttct tgctctgtca tgggggctta
     4801 ttggctatgc cggaatactt gatctgggga taggtcgagc tgttactcag aaactctcct
     4861 ctattcgtgg ctctgatgag gagatgaatt catacacaat aatgagtaca gctattcgga
     4921 tcaccgccat tgttggtggg ctgggctttt ccctgattat catatttggc ctcctggggg
     4981 gcggagaatt tttttcccat aaagatgtca gttcgcttga gctagaattc tctgttttac
     5041 tattggcttt tgccttacct ttgcaagcta taagtgctac ctatagaggg gtgaatgagg
     5101 cgtatctcaa cttcaaaggg atcaacctct tacgtatctt tcttggggcg actaattttg
     5161 gtggtccttt tctagtttct ttctatgcaa aggatcttca ttatcttgta actacccttg
     5221 tcctgtctcg ggctttggct ttctttatat tccggcgttt agctcatgga gtactcaaag
     5281 aaacactggt ggagagaagt tgcaaatatg atcggcgtca ggctgttgag ttgttccgat
     5341 ttggtggttg ggtcactgta agtagtataa taaatccttt tcttgttcag tccgataggt
     5401 tttttattgg tgttttattg tctgctgcag cggtaacctc ttatgtaatt ccatatgaaa
     5461 tcactattca gtcgatgatc cttgtgggtg ctgtttccac cgttgccttt ccgtctatat
     5521 caaatttaat tcgcacttcc tttgctgaag ctctggagtg ttttaataaa tggctcttgc
     5581 gtgtgctgtt gataatggga ggagggatgc tttgcctggc ttttcttttg ccctttattt
     5641 tgaagttgtg ggtgggggat tatatagggg acgactctat cagtgtagga aggatccttt
     5701 gtcttggagt gtttttcaat gcgttgggtg ctatgttcta ttctttcttg catgccaacg
     5761 ccaaggtgaa agaaactgca attttgcact ccatagagct accaatattt atcctcattt
     5821 tgattattct gattccacgg catgggatcg tgggggcggc tgttgcgtgg tcgctgagaa
     5881 cattggcgga taccatagtg cttgctgttt tgagctattt ttcaggctgg agagtgaaat
     5941 ttgctaactg ctagtgcaag agtcgccagc ttttttattg ctactcttct gattttttct
     6001 ttggcttttc ctacggcttt gatcggtatt aaatttattt tactgatctt agcattattc
     6061 tttacgctta tctctagcct cgctcgggct gatctgaatc tatatgtgct attggctgga
     6121 ttgttttata gcggtgtagg gttggtttgg tctatttatg gtgagttgag ggggaatgca
     6181 ggggctctac aggtacttac cgtcatggtg gtatatcctg ttctcttcgt tttgctgaca
     6241 atttcttata gagggcaatc tgaggctctg ggtaagttat ttttttacac ggcatttttg
     6301 atagctgttc ttgatatatt tttcgttttt ggtaattacc tttttcccta caatccagtc
     6361 tatgagtcca tggctgctat gtatggcgat gcagctgtag tggataacgc gggaagatat
     6421 ataaagttta cgcttcccaa tgtttcatcc ttgatattct tgattcccta tgtggtcagc
     6481 tatcttctgt gccggaaatt ctctctttcc ggtctatttc ttttggccct tttgctactg
     6541 gtagcatttg tttcgggcag gagagcactg ttgcttgttg cttttacggc acctttcttg
     6601 gcttattctc ttaccatggg cgccgaccgt tttcaatgga aagtaatttc tcgtgtttta
     6661 ctggttttta tagttttcac gttgggtttt tgatttttta taatacgtat cctgactatg
     6721 tcgaaaacct ttttttatcc ataagtaatt tttcagataa cgatagcaat cttgagcggc
     6781 ggtatcagtt cgaagccttg atgaagggag tctatcagca accattcttc ggaatgggcg
     6841 caggagctat tgctgattat attcgttcgg aagaaatgcc gtgggcgtat gagcttttct
     6901 atgtttcact tgtttttcaa tatgggatgc taggtttctt gttctatgct ttgggcgtga
     6961 tttttttgct tgcctttcta gttttgcgtg tgagaaaaat tggaaggaga agctttgagt
     7021 tttgctatct atccggcatg ttagcattcc taatggctac ggcaaccaat ccatatttag
     7081 caaaatttga ttatatgtgg gtgatattta ttccggtagc attgcttaat tacagtgccg
     7141 tttctagggg gcagggtgaa aagtccaatt attgatgttg tcgttgttaa ctggaatgcg
     7201 ggccttcagt tgcgttcctg tgttgactcc atttttaaaa actctttcgg actcataaga
     7261 aaagtcattg tgatcgataa tggctctacc gataactctt gtgcccttct tggtgagcgg
     7321 gagaatcttg aggtcgtgtt gacaggagag aatctcggtt tcgctacagc ctgcaatctc
     7381 ggagcttcca aaggagaggc tgaatatata cttttcctga atccagatgc ggaactctat
     7441 gaagagacga taggttctgt tgttcgcttc atgaacagat ccgaaaactc taaggtagga
     7501 gtttgtggag tgcagttaat cgatgaacaa ggacaggtta gtcgtagctg tgctcgattt
     7561 ccctcttccg caagaatgtt gatgcatact atgggcgttg atcgagtctt tcctgctctc
     7621 ggatatgcaa tgactgattg ggatcacttg tcatccaggc ctgtagatca tgttatcgga
     7681 gccttctact ttgtaaggcg gcatgttttc aagctggtcg gtggctttga tgagcgcttc
     7741 ttcgtctatc tagaggacct tgatctgtct taccgtatat cctgttctgg atggagtagt
     7801 ttttaccttt ccactgctca ggcattccat gccggtggag gcgcctcaag gcagatcaag
     7861 gcgcggcggc ttttctattc cctgagaagc cgacttatct atatttttaa gaaccttccg
     7921 gtcgtttctg cgtttttgat tctgttgtcc agcttgtttt ttgagcctgt cattagatct
     7981 atgttttcca tggttaaact ttcccttcct gctcttcggg aaacctggtc ggcttattta
     8041 atgctttacc gctggttgcc gaagtggatt ttctttaacg ctacgcgata gctattatga
     8101 aaatattgtt gctttccaag tatccgcgcc taggagccag cagccgactg cgaactctcc
     8161 aatatatcga ttatctagag tcgaaaggag gaagggtgga tgttagaagt ttttttgatg
     8221 atggctacct gtctggcctc tattcctcgg ggcgtcgttc tttttgtaat gttttaaaat
     8281 catacatttc acgttttttt gtacttttca ctgtctatag atacgattta atctggattg
     8341 aaaaagagat ttttccgtat tttccttctg tggctgagag gtttttaaga ttttttggaa
     8401 agccttatgt ggttgactat gatgatgcca tttttcataa ctatgatctt tctaggaatt
     8461 ggttggtcag aaaatttctc gggaaaaaaa ttgatagcgt catgcgccac tcctcatgtg
     8521 tgatcgcagg taatgactac ttagctgagc gagcccgggt tgctggttca tctcgagtgg
     8581 aaattattcc tacagtgatc gacctcaagc gttacgaggt cagagatgag tcttgtttta
     8641 agaaaacagt cataggctgg attggttctc cttcgactca gaagtatcta ctcgatattg
     8701 cccccgagct agtgaagctc tgtgtcgaat atcaggcatc catgctcctt gtaggggcta
     8761 cacccgagat atcatcctac ttctcaggcg tctatgtgga tgtcaaggcg tggaaggaag
     8821 aagatgaggt gggatatatt cggcagatga caattggtat aatgccgttg cccgatggcc
     8881 cttgggaaaa aggaaagtgc ggatataagc taattcaata tatggcatgt ggcgttcccg
     8941 tcgttgcctc gccagttggt gttaatattg aaatcgtcga aaataatgaa tcaggacttc
     9001 tggctgaggg gagtagagga tggcgtgaat ccttgcagaa gcttctgcag tcttccgaac
     9061 tcaggcggga gtgcgggcgg aacggacgtt cctcggtaga aaaaatatat tcccttcaag
     9121 ttcagtcccg ccgtcttcat cagacattta ttgaagtttt gagttagtca ccggtctatt
     9181 gagtctgctt gatggggatt ttggggtaaa gttaggtatg agcgctaaaa gctgtcagga
     9241 gcctgtatga agccggtttt gctaatggtg ataaacgatc cggcgttctt tatatctcat
     9301 cgtctaccta ttgcgacagg agctaggagt tccggtttcg atgtgcatat tgcgaccagg
     9361 cccggacagg ctgtatcaag gctcatagcg gaaggttttc ggcatcatga actgcctttg
     9421 tccagaagtg gaaaaaatcc tttttcggag ttttatctct tgctgtctat atggcgtctg
     9481 ctatggaagc tacgccccga tattttgcac ctggtcacga taaaaccggt cttatatggc
     9541 ggtatcgctg cccgtctggc tccggtgaag ggagttgtag cggccgtttc tggacttggt
     9601 ttcgtcttta tggccaaggg ctcgaaggcc agtgtgtttc gctcagggat cgcttggctt
     9661 tatcggcggg ccttgggcaa gaaaaggttg cgcgtcatct ttcagaatcc cgatgatcgg
     9721 gatgcgctga ttggcttggg ggcgatcact tttgaaaagt ccgtcctgat tcgcggctct
     9781 ggagttgacc tgtcgctgtg ccatccggta ccggaaagtc ctggtgcgcc ggtggtcact
     9841 ctggcagctc gcttgctcag agataaaggt gtagtggagt tcgtcgaagc ggcaaatatc
     9901 ctcaggcagc gtggtgtgtc agcgcatttc cagttggtcg gggatttgga tccggggaat
     9961 cccagcagta tcgaagagtt cgacctggag cggtggcgta gcgaaggtac ggtcgagtgt
    10021 cttggttatc gtcaggatat tgcgtctgtc ttcgctcatt cgcatatagt tgtcttgcct
    10081 tcgtatcgcg aaggacttcc caaggtcctg gttgaggctg ctgcctgcgg tcgcgtagtc
    10141 gtgacgacag atgtgccggg ttgccgggac gccatcgaag cggatagaac cggcctgctg
    10201 gtgccagttc gtgacgcagt ggcgctagct gacgccattc agcgtcttgt cgaatctccg
    10261 gagctgcgga agaagatggg ggctgctggg cggacattgg cagagcgtga ttttgcgatc
    10321 gaaagtatcg ttcaacaaca cctcgatatc tatcgggcac tagggagcgg agcttgagtc
    10381 gacacaatgt attggtgacg ggcgctaccg gattcgtcgg ggctgcgttg gtcgagagtc
    10441 tttgctcttc ggaaaagtac agggtctggg ccggttaccg tcgagagagt agtccctggc
    10501 ccgagggggt tacccccttg ttcttggggg agctgggtag ttccgtggcc tgggagacgg
    10561 aatcggttat cgacacggtg gtccattgtg ccgcgcgtgt gcatgtcatg agcgagacgg
    10621 tttccgatcc gctcgccgag tttcgcaagg ccaatgtcct gggtaccctc gatttggccc
    10681 gtcaggccac atccctggga gttcgccgtt ttatctatct cagctcgatc aaagtcaatg
    10741 gcgagggggg cgagcccggg cagccctacg tcgccgatag cctgcctaat ccagtcgatc
    10801 cctatggtgt ttccaagctg gaggcggagc aggcgctcct gggcctggcg gcggagaccg
    10861 gcctcgaggt tgtgatcatt cgtcctgtcc tggtgtatgg ccctggggtc aaggccaacg
    10921 tgcagaccat gatgcgctgg ctgaagagag gggttccgct ccccttgggg gcgatacaca
    10981 atcggcgcag tctggtggcg ctggataact tgaccgattt gatcgctacc tgtatcgatc
    11041 acccggcagc ggttaaccag gtctttctgg taagcgacgg ggaggatctt tccactaccg
    11101 acctgcttcg gagaatgggg aacgcgttac gtacgcccgc gcgcctggtg cctgttcccg
    11161 ccagttggat cggcctggtc gccaagacgc tgaacaagca ggcgtttgct cagcgcctgt
    11221 gcggttcgct gcaggtcgat atagaaaaaa accgtcggat gcttggctgg acgccgcctg
    11281 taggcgtgga cgaggctttg gaaaaaaccg cccgctattt cctggatcac caatgatgag
    11341 tctgtggttg ttgttgccgg cggttgccgg cctttccctc ttgctgaccg cggggctgcg
    11401 tcgttatgcg attgctcgca gtctgatgga cgtacccaat gcccgaagtt cccaccaggt
    11461 tccgacacca cgaggcggcg gtgtcgctat cgtgctttcc ttcctgctgg cagtcctgtt
    11521 ggcggccttc ctgggcatga tggatataag tctggccatc ggcatcctcg gcgcaggcgt
    11581 ggggaccgca ttgctgggtt ttctggatga ccatggtcat atcgctgccc gttggcgtct
    11641 gctcgggcat ttcgccggcg cctgctggct gctgtactgg ttgggaggcc tgccggcgct
    11701 ggtctttttc ggttttatga tcgacctagg gtgggtcgga catataggtg cggtgttcta
    11761 cctggtctgg atgctcaatc tctacaattt catggatggt atcgatggga ttgccagcgt
    11821 cgaggctgtg tgcgtgtgca ttggcgctgt tctgcttgtc gtcgtcagtg gcgtcggttc
    11881 cgatgatgca tcccaaggca tctggttgtc ggcactactg gcgtccgcgg tgacgggatt
    11941 cctcttctgg aacttccccc cggcgcggat cttcatggga gacgccggta gcggtttttt
    12001 gggaatcatc cttgcgggcc tttccctgca ggctgcctgg gtctcgccgc aactcttctg
    12061 gggatggctg atcctgctgg gggttttcgt cgtcgatgcc actctgaccc tgctgcgccg
    12121 attgctgcgg ggagataagg tatatgaggc acatcgcagt catgcatatc agtacgcctc
    12181 tcgttattac ggacgtcacc tgcctgttac cctggcggtt gggtgtatca atcttgtctg
    12241 gttgctgcca ttggcgctat ttgtcgcgtc ggggcgaatt gacggcatgc ttgcgttgtt
    12301 gatcggctat ctgcctttgg tggccctggc ccttcggttc aaggccggaa ccctggaacc
    12361 acgcgttgcc tgaatgcccc ttcggctgga ggagcgggct ggctctgcag aaacgtatat
    12421 tatgaaggaa atgtgcaggc gattatctag tgtgtgaata ccgctaatat cgcgacttgg
    12481 attccggaat ggcctgtgcc aggccgagga aatggattgc ccgggttcgt ttgggtggat
    12541 gcgggtgcat tgaaggggct gattaatagg atgttggata acttgcgtgt acgcctcttg
    12601 ggattgccac gccgccaaaa gcggattctc caggtcgcga cggacatcgc cctggtatgg
    12661 ctttcattgt ggctggcctt tctggtgcgc cttggcaccg aagacatgat cagccccttc
    12721 ggcggccatg cctggctgtt catcgccgcc ccgttggtgg ccattcccct gttcatccgc
    12781 ttcggcatgt accgggcggt gatgcgctac ctaggcaacg acgcccttat cgcgatcgcc
    12841 aaggccgtca ctatttccgc gctggtcctg tcgttgctgg tctactggta ccgctccccg
    12901 ccggcggtgg tgccgcgttc cctggtgttc aactactggt ggttgagcat gctgctgatc
    12961 ggcggcttgc gtctggccat gcgccagtat ttcatgggcg actggtactc tgctgtgcag
    13021 tcggtaccat ttctcaatcg ccaggacggc ctgcccaggg tggctatcta tggcgcgggg
    13081 gcggccggca accagttggt tgcggcgttg cgtctcggtc gggcgatgcg tccggtggcg
    13141 ttcatcgatg acgacaagca gatcgccaac cgggtcatcg ccggtctgcg ggtctatacc
    13201 gccaagcata tccgccagat gatcgacgag acgggcgcgc aggaggttct cctggcgatt
    13261 ccttccgcca ctcgggcccg gcgccgagag attctcgagt ccctggagcc gttcccgctg
    13321 cacgtgcgca gcatgcctgg cttcatggac ctggccagcg gccgggtcaa ggtggacgac
    13381 ctgcaggagg tggacatcgc tgacctgctg ggg
//
