LOCUS       AF498415               14364 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 04 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498415 AC104734
VERSION     AF498415.1  GI:20560027
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975263.
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="04"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27777.1"
                     /db_xref="GI:20560028"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             241..522
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27778.1"
                     /db_xref="GI:20560029"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEV"
     CDS             535..828
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27779.1"
                     /db_xref="GI:20560030"
                     /translation="MLLGVFMLLRRLAIAGFFFLLALIVIVFVLENLDSSHVTFLGRQ
                     SPEFPLVLLLLSSFVMGGLLVLLMSLPGYMRTRSMLSGLRTEVAKLRTSPLDH"
     CDS             1098..2132
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27780.1"
                     /db_xref="GI:20560031"
                     /translation="MTTERSPRVVPIDGQIDLVELAQGILRQKWLIASFFLLATALAV
                     VYAFYSRPIYEARASVLPPSLNEISGFNLGRKESDLGVFSVKDIYDVFIRNLMAEETR
                     QNFFKEKYLPTLGDKGAESLDALYNKFNDELQIKASVDGSGRYLISAVAHESDTAAYL
                     VDSYIEMVAGISKNEMLKNIKRDLHVKSVNLEQQINIMRAMAKTRREDTILRLREALK
                     IAEALKIENPPSFEGQTERQLSSIMIGELMYMRGAKALRAEISSLEARESDDSFIGGL
                     RALQERYALYTKLSPDESQVSVYRKDGVVETSDKPIKPKKLIIIVLGAVFGIFLGCLV
                     GLLRIVQFRR"
     CDS             2231..3463
                     /note="ORF_5; similar to Polysaccharide biosynthesis
                     protein; potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AAM27781.1"
                     /db_xref="GI:20560032"
                     /translation="MSLARNTFWNFLGVLIPSLAALPAIGYLARVLGVENFGLLTLSF
                     AIIGYASIFDLGLSRAVIREVALSSDKGSEVNKAVGTSSVVVTIAGFTCALLLYFNSE
                     RLASSLGVTAGNWPDVVLGLNYLSFAIVPLLLSTVWFSFLEGRGDFRNLNFLKIISGL
                     AVAIIPAAWVWISGDERFSVAMLGLVASRIFVMLLAYVFCVLPFGLRVHVFDYFTFKR
                     LINYGGWITVSNFISPVMVYFDRFFISSHLGAATVALYSAPSEVIGRVAFVPTSISRV
                     IFQRLSLDSSDSIQTRVAYKMTFVVCGLSSALIFFGSDIILSVWLGDKYLGEASLVLK
                     ILAVGFLFNSLAQIPFAKIQAEGNSRLTAILHIFEVLPYLALLYFLVLHSSIVGAAIA
                     WTVRVSVDFLLLEYFSRK"
     CDS             3558..4757
                     /note="ORF_6; potential multiple membrane spanning
                     domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AAM27782.1"
                     /db_xref="GI:20560033"
                     /translation="MGGSGAWSEKLIGFSFRKLLFVLISLFLILIFLLERKCLSIIFY
                     LSLVSVFFIVWGGVLPYLNDYWGRNFQDASSLFGLTALCIVAVIGSDRVLLSIVSAAF
                     CFSFVLAVLHCILYFGFSAGAIDVSWVNDFFYQYLAGKGEIFIFTKEPSIRVFWASTS
                     FILVGYTIALFGFLKSNGFFRRLLILFVLWIFVFAAYASDTRALLGYMAFVPVSYVFF
                     RVAAYLLYPFSSGVKIALYVFGMVMVELLLLLAVTPSVLKVLGLNKEGEGTERADQIE
                     AIYHTLSENLFWGIGLGGNSEYLIRSEQAPWSYEMSLWALVMKLGLIGSAILLSILYM
                     ALLIPRAGFVPGFEKSKLVRFLCAVGYGYLIVVSTNPFLFSLIGFATIMVFYSWYVYA
                     GRKNVGC"
     CDS             4744..5631
                     /note="ORF_7"
                     /codon_start=1
                     /gene="orf4"
                     /transl_table=11
                     /protein_id="AAM27783.1"
                     /db_xref="GI:20560034"
                     /translation="MSAAKNLRLYAVIVAYFPEEHALSSLVRSLVSQGCKGVVVFQNS
                     LLSSAFMDEHSDACTFLGEGENLGLGTAINLSFDHLKSKGADAVFTFDQDSSLHEDFV
                     QHMRAMYQGLTDKAIPFSAVVPRIVDRRSLDFEYILPEIQQKELPEGLKFLRIGLQSG
                     MLINYETWWEHKFDGGLFIEYVDTEWCYRLGYEGRYLCLAEEAIMYHEVSDELPKKFL
                     GFYFLKYSPVRRYYFFRNSIFLLRQSYVPAYFKLRILTAFANRIVSMILFSDDRRRSF
                     SLTVKGILHGLGSVKGPLL"
     CDS             5639..6943
                     /note="ORF_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /protein_id="AAM27784.1"
                     /db_xref="GI:20560035"
                     /translation="MVKKVLIISNNALSFSANNGRTLNSFFADYDGEKLAQLFFNAER
                     PQSKVVKRFFNVRAFEQLRQLFSKEHEAGSDVSPSVSSVVTTAHGLVSSSVKVRFVSF
                     LNTYLESLKLYLRDLGYRCGLQKNKRLMAWVENFSPECIFFVAGNCKFAIDYALFLSA
                     RLNIPLFTYITDDYVLSNCPVGPFGKAYHHGLHKRYAELLQSSRQVFFIGDSMRSAFE
                     SAYGSGGETLINCNLISQPVPAGREFVTPGQVRVVFAGGLHLGRDSSIISFARLMSGV
                     CGQLGLSYSIDLYSGQSFSSAMLSRFEASGISYRGSASWDDLQVEFKKADFLLHVESF
                     SEKYAEKTRYSLSTKLPEYLSSGVCVIGFGPETVASIKLLRDHKIGLALSKNAGDMNV
                     LSDAIQSTFIRSSFGEQGVSFAWANFSPELIKKKLYDSLQEV"
     CDS             6945..7979
                     /note="ORF_9; similar to Polysaccharide biosynthesis
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /protein_id="AAM27785.1"
                     /db_xref="GI:20560036"
                     /translation="MFSSKKLLITGGTGSFGNAVLKRFLDTDIAEIRIFSRDEKKQDD
                     MRKRYGSAKLKFYIGDVRDYQSVLNAMRGVDFVYHAAALKQVPSCEFHPMEAVKTNVI
                     GTDNVLEAAIQSDVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNLETTSTVIC
                     GTRYGNVMASRGSVIPLFIEQMRSGKPLTITDPSMTRFMMTLSDAVDLVLYAFEHGNN
                     GDLFVQKAPAATIEVLATALTGLLGKEGYPVNVIGTRHGEKLYEALLSREEMACAEDM
                     GDYYRVPPDLRDLNYSKFVEQGEERISHTEDYNSHNTDRLDVKGMQELLLKLDFIRAI
                     QRGEQANPEE"
     CDS             7983..9098
                     /note="ORF_10; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27786.1"
                     /gene="orf7"
                     /db_xref="GI:20560037"
                     /translation="MRVLITGANGFVGKNLAAHLAERNDVEVITFTRENSIESLPGIL
                     GSIDFVFHLAGVNRPENPEDFKSGNANLTKALCDAIRRTGRSIPLLYTSSTQAELQNK
                     YGLSKRDAENYIRELSLDTGSPVYIFRLPNVFGKWSRPSYNSAVATFCYNIARDIPIQ
                     INDPTAQITLVYIDDVVKKFLAILDAPASREHETRIEPQYKITVGDLADQIHAFRDSR
                     KSLVTERVGVGLVRALYSTYLSFLPEDRFSYELPMHGDARGVFVEMLKTPDSGQFSFF
                     TAHPGVTRGGHYHHSKTEKFLVIKGSARFKFRNISTGAFYELYTSGEKAEVVETVPGW
                     THDITNVGSEEMVVMLWANEIFDREHPDTYACPVGEK"
     CDS             9099..10250
                     /note="ORF_11; similar to UDP-N-acetylglucosamine
                     2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27787.1"
                     /gene="orf8"
                     /db_xref="GI:20560038"
                     /translation="MTCSEALMKKLKVVTVVGTRPEIIRLSRVMAKLDEYCDHVLVHT
                     GQNYDYELNEIFFQDLGIRKPDHFLNAAGASGAETIGNVIIAVDRVLSVEQPDALLVL
                     GDTNSCMAVLPAKRRKIPTFHMEAGNRCFDMRVPEEINRRIVDHTADINLTYSTIARD
                     YLLREGLPPDMVIKTGSPMFEVLNYYRDGIESSDVLARLGLEENRFFVVSAHREENVD
                     SERNFLSLVETLNEISEKYDFPVIVSTHPRTMKRVEAMGVKFHANVRLLKPLGFKDYN
                     KLQLSAKAVLSDSGTINEESSILNFPALNIRQAHERPEGMEEAAVMMVGLGRERVMQG
                     LEILESQSRGANRLLYLVHDYSCDNVSEKVVRILVSYRDYVMRTVWKKY"
     CDS             10260..11513
                     /note="ORF_12; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpI"
                     /product="glycosyltransferase"
                     /protein_id="AAM27788.1"
                     /db_xref="GI:20560039"
                     /translation="MDKAKLKVLVVTQYFWPENMRINDLVSDFVGRGHEVTVLTGCPN
                     YPEGRVFSGFKENPASYSCYLGASVIRVPMLPRGLRSIQLALNYLSFFMSASFFGAFR
                     LRRRKFDVVFVYAVSPIMVAIPAIIIGRLKRAPVFVWVLDLWPETLRAVGVLNSDRAL
                     SIVGKMVSWIYNRTDYVLVQSKAFMDSVAKYCTRTIPEGRIIYFPSWAEDDFSNSESA
                     NSALLERDDSVFTLLFAGNVGEAQDFPSILDAAERLRQDVPMRWVIVGDGRASAWLKA
                     EVAARGLDNVLLLGRHPLEEMPPLFALADALFVSLKTNEVFEKTIPGKVQAYLASGKP
                     VIAMIDGEASRVISEAGAGYACASGDVEALVDIIRRAAALTGEQREGLGRAGREYYEC
                     NFSKQALLSRLEGLFIDARLRRDEK"
     CDS             11510..12466
                     /note="ORF_13; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27789.1"
                     /gene="wbpK"
                     /db_xref="GI:20560040"
                     /translation="MNKVLLTGASGFVGKALAGELLRQGYALNMAFRQIPSKVLDGAV
                     AIPVGNIEGGTLWSDALAGVDTVIHSAARVHVMNDQALDPLAEFRKVNVEGTLNLARQ
                     AAAAGVKRFVFVSSIKVNGESTTTGSPFKPDDVPAPSDPYGISKLEAETGLRQVCEEA
                     GMEYAIVRPVLVYGPGVKANFLNMMKWLDKGIPLPLGAINNRRSLVSLTNLVDMLVTV
                     ASHPAAANQVFLVSDGEDLSTTGLLRRMAVAMGKRANLLPVPESLLKTAALLLGKKSV
                     GQRLCGSLQVDISKNKQLLDWNPPLSVDQGLQRAAEYYLEYR"
     CDS             12468..13487
                     /note="ORF_14; similar to Glycosyl transferase group 4;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr2"
                     /product="glycosyltrasferase"
                     /protein_id="AAM27790.1"
                     /db_xref="GI:20560041"
                     /translation="MSIWWILFPVVLVSAGLTGLLRFYAISKKVMDVPNARSSHTVPT
                     PRGGGVAIVLTFLLVAFLLPLFSILEWRQALLFLLGGGGVALLGFLDDHGHVAARWRL
                     LGHFMAALVSLFLLAGVPPFKLLGYQWDLGWVGVVLFAFYLVWLLNLYNFMDGIDGLA
                     CLEAIFVCLGGALVYWMNGKTALSLIPLLLAFAVLGFLFWNFPPAKIFMGDAGSGFLG
                     LTLGVLSIQAMWVEPEFFWAWLILLGVFIVDATYTLARRLLRGDKVYEAHRSHAYQYA
                     SRHFGKHAPVTLGVTGLNLVWLLPMALWVASGHLEPALGILIAYTPLVFLAVKFKAGK
                     LESVG"
     CDS             13555..>14364
                     /note="ORF_15; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpM"
                     /protein_id="AAM27791.1"
                     /db_xref="GI:20560042"
                     /translation="MPRRWKRLLQVATDILLVWLSLWLAFVVRLGTDDMIDIFGEHAW
                     LFIAAPLIAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSLVVYWYRGAPAP
                     VPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDGLPRVAIYGAGA
                     AGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMIDETGAQEVLLA
                     IPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDIADLLG"
BASE COUNT     3097 a   3047 c   3952 g   4268 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgta agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga tctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 gaaaaagggc ggcctaggcc gccctttttc gttttcccct tcttggacct gttcaaagac
      181 tgatcagcat gctaaaagag acctgagctg atctagccgc ttgaaaaaga agggaaaacc
      241 atgaccaagt cggagttgat cgaacggatc gttacccatc aggggcaact gtccgcgaag
      301 gatgtcgagt tggcaatcaa gaccatgctg gagcaaatgt cccaggccct ggcgaccggg
      361 gaccggatcg agatccgtgg tttcggcagc ttttccttgc actaccgcgc cccgcgcgtc
      421 ggtcgcaacc ccaagaccgg ggagtcggta cgactcgacg gcaagttcgt gccgcacttc
      481 aagccgggca aggagttgcg ggatcgggtc aacgaggttt gaattggctg aaacatgtta
      541 ttaggagtct ttatgcttct caggcgtctg gctatcgctg gctttttttt cctattggcg
      601 cttatagtca ttgtcttcgt attggagaat cttgacagta gtcatgtgac gtttttgggg
      661 aggcagtctc ctgaatttcc tttggtgctc ttgctgctat cgtcttttgt tatggggggg
      721 ctgcttgttc tactaatgag tcttcctggc tacatgcgca cacgcagcat gctttctggc
      781 ttgcgtacgg aagtggcgaa gctaaggaca agtccgctgg atcactaaag gcggcggaaa
      841 ggctttgccg ggagggtgat tgctcgaggg gtgtaagggg cggaggaaac taaccttctg
      901 tgtggttgtc tatgcatgtt gagctattta tctcgacact gtcggatgaa agggcttgtc
      961 gaaggaggct ttcggttcgt actccttgta ttctcgagtc atgctggcaa aatgcagtag
     1021 cttggagagc atggagctgg agaacgattt ctgcctttga tcggaactcc taaccaaaat
     1081 caagtgatgg atgcattgtg accacagaaa gatcaccccg tgttgtccct attgatgggc
     1141 agatcgacct cgttgaactt gcacaaggta ttctcaggca gaagtggttg attgcctctt
     1201 tttttcttct tgcaacggcc ctcgccgttg tttatgcatt ttatagtcgg ccaatctatg
     1261 aggctcgagc ttctgttttg cccccttcct taaatgaaat atctggtttt aatcttgggc
     1321 gaaaagagag tgacctggga gtcttttctg tcaaggatat atatgacgtc tttatcagga
     1381 atttgatggc tgaagagaca cggcagaatt tcttcaagga aaagtatcta ccgaccttgg
     1441 gagacaaagg ggcggaatct cttgatgcat tatataataa gttcaatgat gaattgcaga
     1501 tcaaagcatc ggtggatggt tcagggcgct atttgatttc tgctgtagct catgagtcgg
     1561 atacagcagc ttatttagtt gactcttata ttgaaatggt cgccggaata tccaagaatg
     1621 aaatgctcaa aaatattaaa agagacttgc atgtaaaaag tgtgaatcta gagcagcaga
     1681 taaatatcat gagggcaatg gccaagactc gcagggagga tactatcttg cgcctgaggg
     1741 aagctctgaa aatagccgaa gccctgaaga ttgaaaatcc accctcgttt gaggggcaaa
     1801 cggagcggca gctatcttcg atcatgatcg gggaacttat gtatatgcgt ggggcgaaag
     1861 ctctgcgggc agagatatct agtttagagg cgcgtgagtc tgatgattct ttcatcggcg
     1921 gattgagagc tttgcaggaa agatatgctc tttatacaaa actatctcct gatgaaagtc
     1981 aagtctctgt ctatagaaaa gatggggtcg ttgaaacttc tgataagcca ataaagccga
     2041 agaaattgat tataattgta ctgggtgctg tatttggtat tttcctggga tgcttggtag
     2101 gcctgctacg cattgtgcag tttaggcgct gaagtagctg atggcataac gcacatcagg
     2161 attatgtcga tctctggagc agattgagct gtgccgattt ttttgaactt atttgcttga
     2221 gggatatggc ttgagcttgg ctaggaatac tttttggaac tttttagggg tactgattcc
     2281 ttccttggcg gcgttgcctg ctatcggtta tctggccaga gttttggggg tcgagaattt
     2341 tgggcttctg accttgtctt ttgccatcat tggatatgcc agtatatttg acttgggttt
     2401 atccagagct gttatcaggg aggtggctct gagttcagat aaaggttcgg aagtcaacaa
     2461 ggctgttggg acttcctctg tagtagttac tattgcgggt ttcacctgtg ccttgctctt
     2521 gtacttcaat agcgaacgct tagcctcgtc gttgggagta acggcgggga actggccaga
     2581 cgtggtattg gggctcaact accttagttt tgcaatagtc cccttgctat tgagtacggt
     2641 ctggttctcc tttttggaag gccgtgggga ttttaggaac ctgaattttc taaaaattat
     2701 ttctggtttg gctgttgcta tcattcctgc cgcttgggtt tggataagtg gggacgaacg
     2761 gttttctgtg gcgatgctgg ggctcgtagc ctctcgtata ttcgtcatgc tgctggcgta
     2821 tgtgttttgt gtgctcccgt tcggacttcg agtgcacgtt tttgattatt ttactttcaa
     2881 gcgtctgatc aactatggcg gctggattac tgttagtaat tttattagcc ctgtaatggt
     2941 ctattttgat aggttcttta tttccagcca tctgggggcg gcaactgttg ccttgtactc
     3001 agccccctcc gaggtgattg gaagggttgc cttcgttccc acgtccatat cccgggtcat
     3061 atttcagcgt ttgagtctgg attcttctga tagtatacaa accagagttg cctataagat
     3121 gaccttcgtt gtatgtggct tatcttcagc tcttatattc tttggtagtg acattattct
     3181 ttctgtatgg cttggtgata agtatctggg ggaggcctct cttgtcctca aaatccttgc
     3241 tgttggcttt ttgttcaatt ctctggcgca aatacctttt gccaaaattc aagctgaagg
     3301 taattccagg ttgacagcta ttttacatat tttcgaggtg ctcccatatc tggctttgtt
     3361 gtattttcta gttctacatt cttccatagt gggggcggca atagcttgga ctgtcagggt
     3421 gtctgtcgac tttctgctgt tggaatattt ctccaggaaa tagagggcta agcgcagtgt
     3481 atataaggct ggatgctctt cgggtcgctg agtttttttt cttgctgttc tctgttgttt
     3541 ttttggcgga aatatcagtg ggcggtagtg gggcttggag tgaaaagctg attggttttt
     3601 cgtttcgtaa actgcttttt gtgttgattt cactttttct aatcctcatt tttcttctag
     3661 agcgaaagtg cctttctatt attttttatc tttctctggt ttcggtgttt tttattgttt
     3721 gggggggcgt tctgccttat cttaacgatt actgggggcg caattttcaa gatgcttcgt
     3781 cgctctttgg tttgacggcg ctttgtattg ttgctgtcat cggttcagat agggttctac
     3841 tgtctatagt ttcggccgct ttctgttttt cctttgttct agcagtactg cactgtattc
     3901 tttattttgg attttctgcc ggggcgatag acgtttcttg ggtaaatgac ttcttctatc
     3961 agtatcttgc tgggaaggga gagattttca tatttacgaa ggagcccagc attagggtct
     4021 tctgggctag cacttccttt atcttggttg gatatacaat cgctcttttc ggttttttga
     4081 aatcgaacgg attttttcgc cgcttgttga ttctttttgt gctctggata tttgtatttg
     4141 cggcttatgc atctgatacg agagcgttac tgggatatat ggcgtttgtt cctgtttcct
     4201 atgttttctt tagggttgct gcttatctgc tttatccgtt ttcctccgga gtaaagatcg
     4261 cgctttatgt ttttggtatg gttatggtgg agttgctttt gctacttgcg gtgacaccct
     4321 cggtactgaa agtgctcggg ttgaacaagg agggggaggg gactgaacgt gctgatcaga
     4381 tcgaagcgat atatcatacc ctgtctgaga atctattttg gggtattggt ctgggtggca
     4441 actccgagta cctgataaga tcggagcagg caccttggtc ttatgagatg tcgctctggg
     4501 cactggtgat gaaactgggg ctcattggca gtgctatatt gctcagtatt ctatatatgg
     4561 ccttgctgat cccgcgggca ggttttgtgc ctgggtttga aaagagcaag cttgtcaggt
     4621 tcctatgtgc ggtcggctat ggctatctca tcgttgtgag tacgaaccct ttcttgttta
     4681 gccttatcgg ctttgcaaca atcatggttt tttattcgtg gtacgtatat gcggggcgaa
     4741 agaatgtcgg ctgctaaaaa cttgaggctt tatgctgtaa tagttgctta ttttccggag
     4801 gagcatgctc tgtccagttt ggtcaggtct ttggtttctc aagggtgcaa aggtgttgtg
     4861 gtttttcaga actcgctgct ttcttctgcc ttcatggatg aacattcgga tgcctgtaca
     4921 tttctgggtg aaggggaaaa cctaggcttg ggaactgcaa taaacctatc atttgaccat
     4981 ctaaagtcaa agggggcgga tgctgttttc acctttgatc aggactccag tcttcatgaa
     5041 gactttgttc agcatatgcg tgccatgtac caaggcctaa ctgataaagc tattcctttt
     5101 tccgctgttg tgcccaggat agtggacaga agatctttag attttgaata tatacttccc
     5161 gagatccagc aaaaagaact tcctgagggc ttgaaatttt tgaggattgg tctgcagtcg
     5221 gggatgttga tcaattatga aacttggtgg gagcacaagt tcgatggtgg actcttcatc
     5281 gagtatgtcg ataccgagtg gtgttatcgt ctcggatatg aagggaggta tctctgtctc
     5341 gccgaagagg ctatcatgta tcatgaggtt tctgatgagt taccaaaaaa gtttcttggg
     5401 ttttatttct tgaaatattc gcctgtgcgt cgttattatt tttttcgaaa ctctatattt
     5461 ctcttgaggc agagttacgt tcctgcttat ttcaagttaa gaatattgac ggcttttgct
     5521 aatcgcatcg tttcaatgat tcttttttct gacgacaggc gtcgctcttt tagtttaacg
     5581 gtgaaaggta ttcttcatgg gttgggctct gtaaaagggc ctttgctcta ggtggggtat
     5641 ggtaaaaaaa gtcttgataa taagtaataa cgccttatct ttttcggcta ataacggaag
     5701 gaccttgaat tctttctttg ccgattatga tggcgagaaa ctggcccagc ttttttttaa
     5761 tgctgagagg cctcaatcca aagtcgtcaa gcgcttcttc aatgtcagag ccttcgaaca
     5821 gctgcgccaa cttttttcca aagagcatga ggcggggagt gatgtttcgc catctgtgag
     5881 cagcgttgtc acaactgcgc acgggctagt ttcaagctcg gtaaaggttc gatttgttag
     5941 tttcttgaat acctatcttg aatccttgaa actgtacctg cgcgatttag gttaccggtg
     6001 tggcctacaa aagaataagc ggctaatggc ttgggtggaa aatttttctc ctgaatgcat
     6061 tttctttgtg gcggggaact gtaagtttgc tatcgattat gcgctttttc ttagcgctag
     6121 attgaacata ccgttgttta cctacatcac ggacgactat gttctttcga attgtccggt
     6181 tggccctttt ggcaaggctt atcatcacgg gcttcataag cgttacgccg agcttttaca
     6241 gtcttctcga caggttttct tcataggtga ctcaatgagg tccgcttttg agagtgcata
     6301 tggctcgggt ggcgaaacac ttataaattg caatttgatc agtcagcctg tacctgcggg
     6361 tcgggagttc gtgactccag ggcaagtcag agtggttttt gcgggtgggc tccacttggg
     6421 gagagattct agtataatat cctttgccag acttatgtct ggagtctgtg gtcaactggg
     6481 tctttcgtat agcattgatc tttactctgg acaaagtttt tcttctgcta tgctttcacg
     6541 cttcgaggct tcaggcataa gttaccgagg atctgcttcc tgggatgatt tgcaggttga
     6601 gttcaagaaa gcggactttc tacttcatgt ggaaagtttt tcggagaaat atgcggagaa
     6661 gaccaggtat tctctttcca ccaagcttcc ggagtatcta tccagtggtg tctgtgtgat
     6721 tggttttggt ccagagacgg ttgcctccat caagctccta agggaccaca aaatagggtt
     6781 ggcactgtcg aagaatgctg gtgacatgaa tgtgctgagc gacgctatac agagtacgtt
     6841 cataagaagt tcttttgggg aacagggtgt ttcatttgca tgggcaaact tttctcctga
     6901 gctgattaag aagaagctat atgattcact gcaggaggtt taatatgttt tcttcgaaga
     6961 aattgctgat tacgggtggt actggttctt ttgggaatgc tgtcttaaag cgattcttgg
     7021 acacagatat tgccgagatt cgaatcttca gtcgcgatga gaagaagcag gatgatatgc
     7081 gcaagcgcta tggaagcgct aagctgaaat tctatatcgg cgatgttagg gactatcaga
     7141 gcgttctcaa tgccatgcga ggcgtggatt tcgtctatca tgcagcggcc ttgaagcagg
     7201 ttccctcctg tgagttccat cctatggagg ctgtgaagac gaatgttatc ggaaccgata
     7261 atgtcttgga agcggcgata caaagtgatg tgaaaagagt cgtttgtcta agcaccgaca
     7321 aggctgtcta tcccattaac gccatgggta tttccaaggc gatgatggaa aaggtgatgg
     7381 ttgcgaaatc acgtaacctt gaaaccactt ccactgtcat ctgtggtact cgttacggta
     7441 acgtaatggc ttcgaggggg tcagtgattc cgctgtttat cgagcaaatg cgttctggca
     7501 aacctctgac cattactgac ccgagcatga cccgcttcat gatgactctc tccgatgcgg
     7561 tggatctggt gctttacgcg tttgagcatg gtaacaatgg ggacctcttc gtgcagaagg
     7621 ctccagccgc aacgattgag gtgctggcca cggcattgac ggggctgctt ggtaaagaag
     7681 gctatccggt caatgtaatc ggtacgcggc atggcgagaa actctatgaa gcgctcttga
     7741 gtcgtgagga aatggcttgt gccgaagata tgggagacta ttatcgcgta ccgccggatt
     7801 tgcgcgatct gaattatagt aagtttgtag agcaaggtga ggaaaggatt tcccataccg
     7861 aagactataa ttcacacaat acagatcgtc tggatgtcaa gggtatgcag gaattgctgc
     7921 tgaagcttga tttcatacgt gctattcagc gtggcgaaca ggcaaacccc gaggaatagg
     7981 gcatgagggt cttaataacc ggtgcaaatg gctttgtagg aaagaacctt gctgctcatt
     8041 tagctgagcg aaatgatgtc gaggttataa cctttacgcg cgagaatagt attgaaagct
     8101 tgccaggaat tcttggttcg attgattttg tttttcattt ggctggtgtc aatcgccccg
     8161 agaatcctga ggacttcaaa tctggaaatg cgaatctgac caaagcacta tgtgatgcaa
     8221 tccggcgaac tggtcgttct attcctctgc tctatacatc ttcgacgcag gcggagttgc
     8281 agaataaata tgggctgagt aagcgcgatg cagagaatta tattcgagag ctgagtttgg
     8341 atacaggctc tcctgtatat atattccgct tgccgaacgt ctttggcaag tggtcgcgtc
     8401 ccagttataa ctctgcggtg gcgacttttt gctataatat tgccagggat attcccattc
     8461 agataaatga tcctacggct caaattacgc tcgtttatat cgatgatgta gtcaaaaagt
     8521 ttttggcaat tctcgatgct ccggcttcta gggagcatga gactcgaatc gagccgcaat
     8581 acaagatcac cgtcggtgat cttgccgacc agatacatgc gttccgcgac agccggaagt
     8641 cgctcgttac ggagcgcgta ggtgtcggct tggtgcgggc cttgtattcc acctatctca
     8701 gcttcctccc cgaggaccgt ttcagctacg agctgccgat gcatggcgat gcgcggggag
     8761 tcttcgttga gatgctgaaa acgcctgact ccggtcagtt ctcgttcttc actgcacatc
     8821 ctggcgtcac tcgtggtggt cactaccacc attccaagac tgagaaattc ctcgtaatca
     8881 agggtagcgc tcgcttcaag ttcaggaata tttccactgg ggctttttat gaattatata
     8941 ccagtggcga aaaggccgaa gttgtcgaaa cagtcccggg ctggacgcat gatataacga
     9001 atgttggttc cgaggagatg gtcgtcatgc tctgggcgaa cgaaatattt gatcgtgagc
     9061 atccggatac ctatgcttgc ccggttggcg aaaaataaat gacttgtagt gaggctctga
     9121 tgaagaagtt aaaagttgtc acggtagtag gtacgcgccc cgagatcatt cgcctctctc
     9181 gtgtaatggc gaagctcgac gagtattgtg accatgttct cgtccatacc gggcagaatt
     9241 atgactatga gctcaacgag atattttttc aagacctggg cattcgaaag ccagatcatt
     9301 tcctgaatgc tgctggtgca tccggagcgg aaaccattgg taatgtaatt attgctgtcg
     9361 atcgagtgct ctcggtagag cagccagacg ccttgctggt actgggagat acgaatagct
     9421 gtatggcggt actacccgct aagcgtcgaa agatcccaac tttccatatg gaagctggca
     9481 atcgttgctt tgatatgcgt gttcccgaag aaataaatcg acgaattgtg gatcacactg
     9541 ctgatatcaa cctcacttat agcaccatcg ctcgggatta cttgctgcgg gaaggtttac
     9601 cgcccgacat ggttatcaaa accggtagtc ctatgtttga ggttttgaac tactataggg
     9661 acggtattga gagttctgat gtgctggcgc ggctgggcct ggaagagaat aggttctttg
     9721 tggtgagtgc gcatcgggaa gagaatgtcg actctgaaag aaacttcctt tcgttggtcg
     9781 agaccctgaa tgaaatttcg gaaaagtatg actttcctgt aattgtttcc acgcatccga
     9841 gaacgatgaa gcgtgtggaa gcaatgggtg tcaagtttca tgccaatgtg cgcttgctaa
     9901 agccgttggg tttcaaggac tacaataagc tgcaattgtc ggccaaggcg gttctttccg
     9961 acagcggtac gatcaacgaa gagtcctcga tcctgaactt ccccgccttg aatatacgtc
    10021 aggctcatga gcggccggaa ggcatggagg aagcagcggt gatgatggtc gggctcggtc
    10081 gcgagcgcgt gatgcagggg ctagagattc tcgagagcca gtctcgtgga gctaaccggc
    10141 tgctttatct tgtgcatgac tatagctgtg ataacgtttc ggagaaggta gtgcggattc
    10201 tcgtaagcta tcgagactac gttatgcgta ccgtttggaa aaagtactaa gttctcgtta
    10261 tggataaggc gaagctcaag gttctagttg ttacgcagta tttttggcca gaaaatatgc
    10321 gtattaacga cttggtctcc gactttgtcg ggagagggca cgaagttacg gttttgacgg
    10381 ggtgcccaaa ctatcccgag ggtcgggtct tttccggttt caaagagaac ccggccagct
    10441 attcctgcta tcttggcgct tcggtgatca gagttcccat gctcccacgc gggctgcgta
    10501 gtatacagct tgccttgaac tacttgtcgt tcttcatgag tgcgtctttt ttcggggcgt
    10561 tcaggctaag aagaaggaag tttgatgtag tctttgttta cgctgtttca ccaataatgg
    10621 tagccatacc tgcgattatc attggacgtc taaaaagagc gccagtattt gtctgggtgc
    10681 tggacctgtg gccagagaca ttaagggccg tgggcgtcct caacagtgat cgggctcttt
    10741 ctatagtagg taagatggta tcttggatct acaacagaac ggactatgta ttggtacagt
    10801 caaaagcctt catggatagt gtggcgaagt attgtaccag gaccataccc gaaggcagga
    10861 ttatctactt cccgagttgg gccgaggatg atttctccaa tagtgaatcg gcgaactctg
    10921 cccttttgga aagagatgat tccgttttta cccttctgtt tgcgggaaat gttggagagg
    10981 cgcaggactt tccctccatt ctagatgccg cggagcgtct acgccaagat gttcccatgc
    11041 gttgggtgat cgttggcgat ggccgcgcaa gtgcatggct taaggccgag gttgcagcca
    11101 ggggattgga taatgtcctg ctgctgggcc ggcatcctct tgaggagatg ccgccgctct
    11161 ttgccttggc ggacgctcta tttgtgtcgc tgaagactaa tgaagtattc gaaaagacca
    11221 tccctgggaa ggttcaagcc tacctggctt ccggcaaacc ggtgatcgcg atgatagatg
    11281 gagaggcatc cagggtgatc tccgaggctg gtgctggcta cgcatgcgct tccggcgacg
    11341 tggaggctct ggtggacatc attcgtcgag ccgcggcgct aactggggag cagcgagaag
    11401 ggcttggtag agccgggcga gaatactatg aatgcaattt ttcgaagcaa gcacttttga
    11461 gtcgcctgga ggggctcttc attgacgcac gcttgagaag ggatgaaaaa tgaacaaggt
    11521 tttattgacc ggtgcctcag ggttcgtggg taaagcattg gcaggtgagc ttcttcggca
    11581 gggatatgca ttgaacatgg ctttccgaca gattccttca aaggtgctag atggggctgt
    11641 cgctattccg gtagggaata tcgagggtgg caccctttgg agtgacgctc ttgcaggcgt
    11701 cgataccgtc attcatagcg ctgctcgtgt tcatgtgatg aatgaccagg ctctcgatcc
    11761 tttggctgag ttcagaaaag tgaatgtcga gggaacactg aatctcgctc ggcaggcggc
    11821 agcagcgggg gtcaaacgtt tcgttttcgt cagctctatc aaggtgaacg gcgagtctac
    11881 gaccacgggt agtccattca agcccgacga tgttccagct ccttccgatc cctatgggat
    11941 ctccaagctc gaagctgaaa ccggtctgcg gcaggtgtgt gaagaggcag ggatggaata
    12001 tgctattgtt cgtcctgttc tggtctacgg ccctggagtg aaagccaatt tcctcaatat
    12061 gatgaagtgg ttggacaaag gtattccttt gcctctgggg gccataaaca atcgccgtag
    12121 tctggtgtcg ctgacaaact tggtggatat gctggttacc gtagcgagcc atcctgctgc
    12181 tgctaatcag gtatttcttg taagtgatgg agaggacctg tctaccactg gtcttttgcg
    12241 aagaatggcg gtagcaatgg gaaaaagagc gaatctgctg ccagtccctg aatcgttgct
    12301 gaagactgct gctctcttgt tagggaagaa gtctgtaggt cagagactct gcgggtcttt
    12361 gcaggtcgac atctctaaga ataagcagtt gttggattgg aatccgccgc tttctgtgga
    12421 ccaaggactg caacgggccg ctgaatatta tttggaatat cgctgaaatg tcgatctggt
    12481 ggattttatt tcctgtggtt ttagtatcgg caggacttac aggattattg agattctacg
    12541 ccatctcaaa aaaagtcatg gatgttccta acgcccgcag ttctcacacc gtgcctacgc
    12601 cgagaggcgg tggggtggct atcgttctga cttttctcct agtggctttt cttctacccc
    12661 tattttccat tctggagtgg cgccaggcgt tacttttcct actgggagga ggcggtgtcg
    12721 ctctgcttgg tttccttgac gaccatggtc acgtggctgc tcgttggcgc ctgcttgggc
    12781 atttcatggc tgcactggtt tcactctttc ttctggctgg tgtgccgccg ttcaagctac
    12841 tgggttatca atgggatttg ggctgggttg gggtcgtact ttttgctttc tatctcgtct
    12901 ggttgctcaa tctttataat ttcatggatg gcatagacgg ccttgcatgt cttgaagcta
    12961 tctttgtctg tttgggagga gcactggtgt attggatgaa tggcaagaca gcgctttcct
    13021 tgatccccct gttgctggct tttgctgttc tcggtttctt gttttggaac tttcctcccg
    13081 cgaagatctt catgggtgat gctggtagtg ggttccttgg actgactctg ggagtcctat
    13141 cgattcaggc aatgtgggtc gagccagagt tcttctgggc atggctgatt ctgctggggg
    13201 tcttcatcgt ggatgcgacc tacacgctgg cacgacgctt gttgcgtggc gacaaggtgt
    13261 atgaagccca tcggagccat gcctatcagt atgcctccag gcatttcggc aagcatgctc
    13321 ctgttacact cggcgtaacc ggactgaatt tggtttggct tctacccatg gcgctttggg
    13381 tggcaagtgg ccatcttgag cctgctctgg gtatcctcat agcttatacc cccctcgttt
    13441 tcctggctgt gaagttcaag gctggtaagc tagagtcggt tggatagagc tcttcagcta
    13501 tagctattga gggagtaggg aatgctagat cgctttagag taaaactgtt gtcgatgcct
    13561 cgtcggtgga agcgcttgct tcaagtggcg acagatatcc tgctggtgtg gttatcgctg
    13621 tggttggcat ttgtcgtgcg tcttggtacg gacgacatga ttgatatttt cggcgagcat
    13681 gcgtggctct tcattgccgc tccgcttatc gccattccat tgttcattcg cttcggcatg
    13741 tatcgcgcgg tgatgcgcta tctcggtaac gacgcattga tcgccatcgc caaggcggtg
    13801 accatctcgg ctctggtgct gtcgctggtg gtgtactggt atcgtggcgc gccggcgccg
    13861 gtgccgcgtt ccctggtgtt caactactgg tggttgagca tgctgctgat cggcggcttg
    13921 cgtctggcca tgcgccagta tttcatgggc gactggtact ctgctgtgca gtcggtacca
    13981 tttctcaatc gccaggatgg cctgcccagg gtggccatct atggcgcggg ggcggccggc
    14041 aaccagttgg ttgcggcatt gcgtctcggt cgggcgatgc gtccggtggc gttcatcgat
    14101 gacgacaagc agatcgccaa ccgggtcatc gccggtctgc gggtctatac cgccaagcat
    14161 atccgccaga tgatcgacga gacgggcgcg caggaggttc tcctggcgat tccttccgcc
    14221 actcgggccc ggcgccgaga gattctcgag tccctggagc cgttcccgct gcacgtgcgt
    14281 agcatgcctg gcttcatgga cctggccagc ggtcgggtca aggtggacga cctgcaggag
    14341 gtggacatcg ctgacctgct gggg
//
