LOCUS       AF498417               14734 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 06 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498417 AC104736
VERSION     AF498417.1  GI:20560066
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 14734)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975265.
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="06"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27812.1"
                     /db_xref="GI:20560067"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             241..525
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27813.1"
                     /db_xref="GI:20560068"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEPE"
     CDS             551..805
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27814.1"
                     /db_xref="GI:20560069"
                     /translation="MQWLGRILTAVFVLVVALVVLVFVLENQNSVSLAFLGWHTPDGP
                     LSLFVSLAFILGGLGGLLSGALLRLRGRRQKSLRSESTSL"
     CDS             1190..2254
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27815.1"
                     /db_xref="GI:20560070"
                     /translation="MTEKNFPSYKAKSGEFDLIEIMSGLWSLRWLIALLAVLPVLLAI
                     VYLFITKPVYEARVSTLPPSLSDIAGFNLGRSENGLPAFSANGVYSIFVRNLQSEETR
                     RQFFRDVYIPSLEEGASVGSRDQLYALLMNSVTIQKPSKEDSERYTVVIERHDPSQAA
                     EWGEKYIGIAVKRSMQEMQEGVRREFQVKNRNIQQQIDILRETAKARREDRISRLKEA
                     LRVAEALKLENPPLIEGQVDQQLSSIMEGGLMYMRGAKAIRAEIKTLQDRVSDDPFIP
                     ALRGLQEKYALLEGLTLNLDKVAVFRQDGTIEVPDDPVRPRKGIVLILSMLAGGFLGV
                     AVALAILMVRGGFRRTVDAA"
     CDS             2364..3632
                     /note="ORF_5; similar to UDP-glucose/GDP-mannose
                     dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpO"
                     /product="wbpO"
                     /protein_id="AAM27816.1"
                     /db_xref="GI:20560071"
                     /translation="MKDLKVAVVGLGYVGLPLAVEFGKKRTVVGFDINQGRIAELRQG
                     IDSTLEVDAAELKEASELSFTFNLQDLQKCNVFIVTVPTPIDEHKQPDLTPLVKASES
                     IGKVLKKGDIVIYESTVYPGATEEDCVPVLEKFSGLRFNEDFFAGYSPERINPGDKEH
                     RVSSIKKVTSGSTPEIAELVDSLYREIITAGTHKASSIKVAEAAKVIENTQRDLNIAL
                     INELAIIFNRMGIDTEAVLKAAGTKWNFMPFRPGLVGGHCIGVDPYYLTHKAQSIGYH
                     PEIILAGRRLNDGMGAYVVSQLVKAMLKRRIHVDGARVLLMGLTFKENCPDLRNTKVV
                     DIVRELAEYNIQVDVFDPWVSAEDAMHEYGITPVGTPSHGAYDGIILAVAHSEFKNMG
                     AENIRKLGKAEHVLYDLKYLLDEDKSDLRL"
     CDS             3670..4695
                     /note="ORF_6; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpP"
                     /product="wbpP"
                     /protein_id="AAM27817.1"
                     /db_xref="GI:20560072"
                     /translation="MMSRYEELRKELPAQPKVWLITGVAGFIGSNLLETLLKLDQKVV
                     GLDNFATGHQRNLDEVRSLVSEKQWSNFKFIQGDIRNLDDCNNACAGVDYVLHQAALG
                     SVPRSINDPITSNATNIDGFLNMLIAARDAKVQSFTYAASSSTYGDHPGLPKVEDTIG
                     KPLSPYAVTKYVNELYADVFSRCYGFSTIGLRYFNVFGRRQDPNGAYAAVIPKWTSSM
                     IQGDDVYINGDGETSRDFCYIENTVQANLLAATAGLDARNQVYNIAVGGRTSLNQLFF
                     ALRDGLAENGVSYHREPVYRDFREGDVRHSLADISKAAKLLGYAPKYDVSAGVALAMP
                     WYIMFLK"
     CDS             4692..4889
                     /note="ORF_7"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27818.1"
                     /db_xref="GI:20560073"
                     /translation="MIFTLVRLDESSVVSMSGAIFAFESRLVLQVLNIRGACDSLFSS
                     RSTFSMIVFLFFTHCFLFFMV"
     CDS             4896..6320
                     /note="ORF_8; similar to Polysaccharide biosynthesis
                     protein; potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /gene="flippase"
                     /protein_id="AAM27819.1"
                     /db_xref="GI:20560074"
                     /translation="MLGKHSLVYFLFKSFPAILTLVGLSVFTRLLSPGEYGVYSLTII
                     VVGFLNTVFLQWVALGVGRYLPECSDDQARARLLGTARAISFLVSLVIIFVTFLLWEW
                     REEIGFSILYYMVGFLCLAQAWHDLNLKIQNAILQPLTYGKMLLIKGAGSFFIGVLLV
                     YFGFGVDGLLLGTLVSLVLATIFFQDAWRGVSWALVDKEQLTRLFAYGAPLTLTFLFA
                     FIVNASDRFFIGAFLGDAAVGVYSVSYDLAQYSVGTVASVVHLAAFPLVMEKLSKSGL
                     PQTQDQLRKTFIFIFAVVSPAACGLAMVAPEISGSIMGEEFREGALKIIPLISLSAFL
                     GALKSFYFDYSFQLASATRVQVVTVAVSAVVDVVFNLILIPEFGIVGAAVSSVMAFSS
                     AILISIFLGRRVFPMPALPGKDAMKIALSVLLMAVSVASFSLESAFFGLVVKVVLGGG
                     VYLAAMIALDVSGMRTFLKSKLIR"
     CDS             6517..7488
                     /note="ORF_9; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27820.1"
                     /gene="gtr1"
                     /product="glycosyltransferase"
                     /db_xref="GI:20560075"
                     /translation="MKALLEGLGTRGIDMHMSEALVSAYPPARRLDRPLWLLRELLHR
                     LPQVIGSYGSDVVILQRELLSTIPTLEFLTKAPRILDVDDAIWLHRRGIAANSIARRV
                     DHIVCGNQYLADYFGQFGRPTTIIPTGVDTLRFLPRRERRENRVIGWSGTSGGYRFLY
                     DIEIPLSHLFRDHPEWKLRIVSDRPPEFKVIPAEKVEFIRWTETNEVETIAGMDIGIM
                     PLADDLWSRGKCSYKMLLYMACGLPVVVSEYGMNRDVLARGFIGYGAVDDEGWYESLA
                     ALVKDPEARVRAGQNGRDIIERHYSLDVVCDLWAMVIGSVAPGKGTR"
     CDS             7493..9376
                     /note="ORF_10; similar to Asparagine synthase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27821.1"
                     /gene="orf7"
                     /db_xref="GI:20560076"
                     /translation="MCGIAGFWNITGTLLGDNARVARQMAAAIHHRGPDESGIWYEAP
                     RAPILVHARLAVLELSPAGSQPMHSDCGRYVLIYNGEIYNHLALRARLSEAGVTHSWR
                     GGSDTETLLACFAQWGVESTLKLTVGMFALALWDRQEKTITLARDRMGEKPLYWGWQN
                     GVLFFASELKALKEHPLFRGDIDRDALALFLRYGYVPAPYSIYKGIGKLRAGSYLVLS
                     ERSLNETCEPAAYWSANAAIEEALSNPFQGTDAEAVDLLESQLRTSISDQMVSDVPLG
                     AFLSGGVDSSTVVALMQQQSSRPIRTFSIGFDEPGYDEAVYAKAVAEHIGTDHTELYV
                     NSKDALDVIPSLPKIYCEPFGDSSQIPTLIVSGLARQQVTVALSGDGGDELFGGYNPY
                     QFTPRVWRMLERFPHSMRRFASAFAQDLPLPEKLGKLRDVFASRTAEELFYRLNSHWR
                     NHEYPVIGAQGHTALLDTPERWPRVDSFQHWMMAMDVQGYMPDDILVKVDRAAMANSL
                     ETRVPLIDHRVFELAWRMPLHMKIRNGKGKWLLREVLYRHVSRELIERPKKGFSVPVS
                     DWLRGPLKEWAESLLDERRLQQEGYLDSRLIRRIWNDHLAGRRDHSRRLWSVLMFQAW
                     LES"
     CDS             9373..10524
                     /note="ORF_11; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr2"
                     /product="glycosyltransferase"
                     /protein_id="AAM27822.1"
                     /db_xref="GI:20560077"
                     /translation="MTTVKVIHVIAGLKKVGGAELMLKRLIETQNGGSEFEHSIISLS
                     DLGEFGQGLIEAGISVDVLGMTSMRDMPRVLLRLIWIFRERRPDIVQTWMYHSDLLGG
                     LAARMAGIGGIIWGVRTTDLQEGGKSTTVLVRKVCAWLSGFLPKYIVCAAEASRRSHI
                     AVGYNASRMLVIPNGFDLTRLQATDEQRSAIRSGAGIEASDIVIGSLGRFHPVKDHAS
                     FVAAAGLLAPRYSRLKFLLVGRELLSSNAELQRLIEATGYAERFILLGERQDVASCLK
                     AMDIFCLHSRTEGFPNVLGEAMAMGLPCITTDVGDAAYLLGNDGVVVPALDPNALGKG
                     IEDLIALDVEGRRALGEAARQRIYSNFTMASASQRFMSLYRDILVKRKA"
     CDS             10568..11698
                     /note="ORF_12; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr3"
                     /product="glycosyltransferase"
                     /protein_id="AAM27823.1"
                     /db_xref="GI:20560078"
                     /translation="MKPVLLMIVNDPAFFMSHRLPVAVGAQQAGFQVHIATRPGDAVK
                     KIVSQGFLHHELPLSRSGKNPFSELYLLTYVWRLLWRLRPDVLHLVTIKPVIYGGIAA
                     RLAPVKGVVAAVSGLGFVFMAKGLKACAFRACVAWLYRRALGKKKLRVIFQNPDDRDA
                     LIGLGAITFEKSVLIRGSGVDLTQYQPAPESPETPVVTLAARLLRDKGVLEFVEAANI
                     LRQRSVPAHFQLVGDLDPGNPTSIEPSELERWRSEGTIECLGYRQDIASVFARSHIVV
                     LPSYREGLPKVLVEASACGRAVVTTDVPGCRDAIQADVTGLLVPVRDSAALADAIQRL
                     IESPELRKKMGAAGRALAERDFAIESIVQQHLDIYRALGSGA"
     CDS             11839..12657
                     /note="ORF_13; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27824.1"
                     /gene="orf10"
                     /db_xref="GI:20560079"
                     /translation="MLLGELGSSVVWDAESAIDTVVHCAARVHVMSETASDPLVEFRK
                     ANVQGTLDLAREAVSRGVRRFIFISSIKVNGEGTEPGRPYTADSPPNPVDPYGVSKRE
                     AEQALLDLAEETGLEVVIIRPVLVYGPGVKANVQTMMRWLKRGVPLPLGAIHNRRSLV
                     SLDNLVDLIITCIEHPAAVGQVFLVSDGEDLSTTELLRRMGRALGAPARLLPVPASWI
                     GAAAKVLNRQAFARRLCGSLQVDIMKTRQVLGWTPPVGVDQALEKTARSFLDRQ"
     CDS             12654..13694
                     /note="ORF_14; similar to Glycosyl transferase group 4;
                     potential multiple membrane spanning domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr4"
                     /product="glycosyltransferase"
                     /protein_id="AAM27825.1"
                     /db_xref="GI:20560080"
                     /translation="MMNLWLLLPAVAALSLLLTAGLRRYAIARSLIDVPNARSSHQVP
                     TPRGGGVAIVLSFLLAVLLAAILGAVKPDLATGILGAGIGIALLGFLDDHGHIAARWR
                     LLGHFAGACWLLYWLGGLPALAFFGLVVDLGWVGHIAAAFYLVWMLNLYNFMDGIDGI
                     ASVEAVCVCVGAALLVVVSGVGSDEASQGVWLAALLAAAVTGFLFWNFPPARIFMGDA
                     GSGFLGVIIGGLSLQAAWVSPQLFWGWLILLGVFIVDATLTLLRRLLRGDKVYEAHRS
                     HAYQYASRHYGRHLPVTLAVGGINIFWLLPLALLVAAGKIDGMLALLIGYLPLAFLAL
                     RFKAGVLESRAA"
     CDS             13892..>14734
                     /note="ORF_15; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbpM"
                     /protein_id="AAM27826.1"
                     /db_xref="GI:20560081"
                     /translation="MLDNLRGRLLGLPRRQKRILQVATDIGLVWLSLWLAFLVRLGTE
                     DMIDPFGDHAWLFIAAPLTAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSL
                     LVYWYRSPPAVVPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDG
                     LPRVAIYGAGAAGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMI
                     DETGAQEVLLAIPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDI
                     ADLLG"
BASE COUNT     2930 a   3461 c   4363 g   3980 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgta agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga tctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 gaaaaagggc ggcctaggcc gccctttttc gttttcccct tcttggacct gttcaaagac
      181 tgatcagcat gctaaaagag acctgagctg atctagccgc ttgaaaaaga agggaaaacc
      241 atgaccaagt cggagttgat cgaacggatt gttacccatc aggggcaact gtccgcgaag
      301 gatgtcgagt tggcaatcaa gaccatgctg gagcaaatgt cccaggccct ggcgaccggg
      361 gaccggatcg agatccgtgg cttcggcagc ttttccttgc actaccgcgc cccgcgcgtc
      421 ggtcgcaacc ccaagaccgg ggagtcggta cgcctcgacg gcaagttcgt gccgcacttc
      481 aagccgggca aggagttgcg ggatcgggtc aacgagccgg agtgactctg gcagatacga
      541 ggttttttca atgcaatggc tcgggcggat tttgactgct gtgtttgtgt tggtggttgc
      601 gctggtggtc ctggttttcg tattggaaaa tcagaatagc gtcagccttg ccttccttgg
      661 gtggcacact cctgacggtc cgctttctct cttcgtgtct ctggctttca tcctcggtgg
      721 gctcggtggg ttgttgagtg gggcattgct tcgtttgagg gggcgtcgac aaaaatctct
      781 tcgctctgaa tcaacctctc tataagttcg tcccttccga actaattgcg tgccagctcc
      841 gcagtgggtc tcttcttgct gaaaagggtt gctgccggcg ggtcgtaacc tctcgtggaa
      901 ggtatcctct ggcctgcttg accccatttt tctgctgtgg gcactaatct ctgactgtct
      961 tgtctaacat acgtccgtgc atcctgaatc gcgagctagg ctctgttatc agcgattggt
     1021 gcgctgaagg tgtttgcgtc gtatccctat ctcctggagt cgatggtcgc gaccgtgcgg
     1081 cttgtaatct cgtagcatgc tgtcaaaatg cagcagcttc ctgatgcatc tccacgtcag
     1141 gaggggggct gccgcatgat gactccttaa cccaagaatt ggatccgttg tgacagaaaa
     1201 aaattttccc tcctataaag ccaagagcgg cgagttcgat ctgatagaga ttatgagtgg
     1261 gttgtggtcc ctgagatggc tgattgcttt gctggcagtg ttgccggtgt tgttggctat
     1321 tgtttatcta ttcatcacca agcccgtcta tgaggctcgt gtctcgacgc tgcctccttc
     1381 actcagcgat atagctggtt tcaacttggg aagatcggaa aacgggcttc ccgcgttttc
     1441 ggcaaatggt gtctattcaa tcttcgtgcg aaatctccag tccgaagaga ctcgccgtca
     1501 gttctttcga gatgtatata ttccttcctt ggaggagggg gcttctgttg gttcgcggga
     1561 tcagctttat gcgcttctca tgaattcggt aactattcag aagcctagca aagaggatag
     1621 cgagcgctac actgtcgtca tcgagcggca tgatccttcg caggcggcgg agtggggcga
     1681 gaaatatata ggaattgcag tcaagcgttc catgcaggaa atgcaagaag gagtacgccg
     1741 cgagtttcaa gtgaagaatc gcaatataca gcaacagatc gacatcttgc gtgaaactgc
     1801 gaaggctcgc cgtgaagatc gtatctctcg actgaaagag gcgttgcgcg ttgcggaggc
     1861 cttgaagctg gaaaatcctc cgttgataga agggcaggtt gatcaacagc tttcctccat
     1921 catggaaggc ggactgatgt acatgcgtgg tgccaaggct attcgggcag aaataaaaac
     1981 gctgcaagat cgtgtctccg atgatccttt cattcccgca ttacgaggct tgcaggagaa
     2041 gtatgctctt ctggaggggc tgacgctgaa tctcgataaa gtcgctgttt ttcgtcagga
     2101 tggtacgata gaggtgccag atgatcccgt ccgtccaaga aaaggcatag tgcttatcct
     2161 aagcatgctt gctggtggtt ttttgggggt tgcggtggct ctagccatcc ttatggtgag
     2221 aggtgggttt aggcgcacgg tcgatgctgc atgagttttt tgcttgtagg gtcggcagaa
     2281 gtttatagtc aatgggattt tcagttttca atccatcgga ttttggttga gaactgtttt
     2341 acatgaagtg aggtgcgcgt taaatgaagg atctgaaggt tgcagttgtt gggctaggct
     2401 atgttggtct tccgcttgct gttgaatttg gtaaaaaacg aaccgttgtc ggtttcgata
     2461 tcaatcaggg gcgaatcgct gaactgcggc aaggcattga ctctactctt gaagtagatg
     2521 cggctgagtt gaaagaagcg tccgaactga gctttacttt caatcttcag gacttgcaaa
     2581 agtgcaatgt tttcattgtg acggttccta cacctattga tgaacataag caacccgacc
     2641 tcacccctct tgtaaaggcc tcggagagca tcggaaaggt tctgaagaag ggcgatattg
     2701 taatttatga gtctaccgtt tacccgggag cgacggaaga agactgcgta cctgttctgg
     2761 aaaaattttc cgggctgagg tttaacgagg acttcttcgc cggttatagc cccgagcgca
     2821 tcaatcccgg cgataaggaa catcgggtct cttcgatcaa gaaggtcact tccggctcta
     2881 cgccggagat cgcggagttg gttgactccc tgtatagaga aatcatcact gccggtactc
     2941 acaaggcgag cagcattaaa gtcgctgagg ctgccaaggt gatcgaaaat acgcagcgag
     3001 atctcaatat tgctctgatc aacgaactcg ccatcatttt caatcgaatg ggtatcgata
     3061 ccgaggcggt cctcaaggct gccggcacga aatggaattt catgccgttc cgcccgggcc
     3121 tagtcggcgg tcattgcatc ggagtcgatc catattatct gactcataaa gcccagagta
     3181 ttggctatca tccagagatc atcctggctg gccgccgttt gaatgacggt atgggtgcct
     3241 atgtcgtttc gcaactggtc aaggctatgc tcaagcgtcg tattcacgtg gacggtgctc
     3301 gcgttctgct tatggggctt acattcaagg aaaattgtcc agacctccgc aataccaaag
     3361 tcgtcgatat tgtccgtgag ttggcggaat acaacataca ggttgatgtt tttgatcctt
     3421 gggtgagcgc cgaagatgcc atgcatgagt atgggattac tccagtcggt acgccatccc
     3481 atggcgctta tgatggaatt atccttgcgg tcgcgcacag tgaattcaaa aatatgggcg
     3541 cagaaaatat acgaaagtta gggaaggctg agcatgttct ctatgatctg aaatatctac
     3601 tcgatgaaga taagtctgat ctacgcctgt aagatcagtt gtaagctttg tgagtagacg
     3661 agagggaata tgatgagtcg ttatgaagag ctaagaaagg aattgccggc gcagccgaaa
     3721 gtctggctga ttacaggtgt ggcgggcttt attggctcta atcttcttga gactttgcta
     3781 aagcttgatc agaaggttgt cggtctggat aattttgcta ctggtcatca gcggaacctg
     3841 gacgaagtgc ggtccttggt tagcgagaag caatggtcaa attttaaatt tattcaaggt
     3901 gatattcgca atctggatga ttgcaataac gcctgtgcag gtgttgatta cgttttacat
     3961 caagctgcct tgggttcggt accgcgttct attaacgatc cgatcacctc caatgcaacg
     4021 aacatcgatg gtttcttgaa tatgctgatt gcagccagag atgccaaggt gcagagtttc
     4081 acttatgcgg caagtagctc tacctatgga gatcatcctg gtttaccgaa ggtggaggat
     4141 actataggta agcctctttc cccttatgcg gttaccaaat atgtgaatga gctttatgcc
     4201 gatgtgtttt cacgctgcta cggcttttcg accattgggc ttcgttattt caatgtgttc
     4261 ggtcgtcgac aggatcccaa tggtgcctat gcggcagtca taccaaaatg gacctcttcg
     4321 atgatccagg gtgatgacgt ctatattaac ggtgatggcg agaccagtcg ggatttttgt
     4381 tatattgaaa acaccgttca ggccaatctg cttgctgcaa ccgcggggct tgatgctcgt
     4441 aatcaagttt acaatattgc tgttggcggg cggacgagtt tgaatcagtt gttctttgcg
     4501 cttcgcgacg gccttgccga aaacggtgtg tcctatcacc gggaacctgt ttatcgtgat
     4561 tttagggagg gggatgtacg tcactctctc gctgatatca gcaaggctgc caaactgctg
     4621 gggtatgcgc cgaaatatga tgtgtctgca ggtgtggcgt tggccatgcc ttggtacatc
     4681 atgtttttga aatgatattc actctggtgc gtttggatga aagtagcgtc gtttccatga
     4741 gcggcgctat ttttgctttt gagagtcgcc tggtgctcca agtgctcaat attagggggg
     4801 cgtgtgattc tctattttca tcgcggagca ctttttctat gattgttttt ttatttttta
     4861 ctcattgttt tctctttttt atggtttaga aaagtatgct gggaaaacat agtctggttt
     4921 attttttgtt caagtctttt ccggcaatat tgactcttgt cggtctttcc gtttttactc
     4981 gtctgttgag tccgggtgag tatggagtct attcgctgac aattatagtt gttggcttcc
     5041 tcaatacggt tttcttgcaa tgggtggcgc tgggggttgg tcgttatctg cctgagtgta
     5101 gtgatgacca agctcgggcg aggcttcttg gcacggctag ggccattagc ttccttgttt
     5161 cactcgttat catattcgtc acgttcctgc tttgggagtg gcgtgaagag ataggtttct
     5221 ctattcttta ttatatggtc ggttttcttt gcctggcaca ggcttggcat gatttgaatc
     5281 tgaagattca aaatgcgatc ttgcagccgc tgacctacgg gaaaatgttg ttgatcaaag
     5341 gggctggaag cttctttatc ggtgttctcc ttgtttattt cgggtttggc gttgatggtt
     5401 tgctgctggg tacgcttgtg tctctggtac tggcgaccat tttcttccaa gatgcgtggc
     5461 ggggagtcag ttgggcgttg gtagacaagg agcagttaac caggttgttt gcctatggtg
     5521 caccactgac gcttactttc ctttttgcat tcatagtcaa tgcgtctgat cgcttcttta
     5581 ttggggcatt tctgggggat gccgcggtag gtgtctactc ggtgtcatat gacctagcgc
     5641 aatatagtgt tggtacagtg gcttctgtcg ttcacctggc ggcttttccg ctggtaatgg
     5701 agaaactttc caagagcggc ttgccacaga cacaggacca actgcggaag acctttattt
     5761 ttatctttgc agtagtctcg cctgctgcct gtggtctggc catggtggct ccggaaattt
     5821 caggatctat tatgggcgaa gagtttcgtg agggtgccct aaagataatt ccgttgatat
     5881 ccctttcggc ttttctgggg gccttgaaat ctttttactt tgactactct ttccaattgg
     5941 ctagtgctac acgagtgcag gttgtgacag tggcggtgtc ggcagtggtt gatgttgttt
     6001 ttaatttaat ccttattccg gagtttggga tagttggcgc ggcggtatct tctgttatgg
     6061 cgttttcctc tgcaatacta attagtatct tcttggggcg acgtgttttc ccaatgcccg
     6121 cccttccggg gaaagatgct atgaaaattg cgttgtctgt gctgttgatg gcggtgtcag
     6181 ttgcatcctt cagtttggag agtgcttttt ttggtctggt tgtgaaagtt gtcttggggg
     6241 gaggggtcta tttggcggca atgattgctc ttgatgtatc tggcatgcgt actttcctga
     6301 agagcaagct aattcgatga taagcagtat gccgacttgc tttctgtttg gaggggatgg
     6361 ggcccggttt ggtggggagt gcccagagca ctgcgatctc ccgttggaca ggcgtgtcca
     6421 ggaatcaagg aggggtaaat catgatagcg gagctcccag cagtgcgggt tcgggctttt
     6481 acccaaggta gatccgttcc cagttcgaga tttcgcgtga aggctctgct cgaagggctt
     6541 ggcacgcgag gtatcgacat gcatatgtcg gaagcgctgg tctctgccta tcctccggct
     6601 cgtcgactgg atcgcccgct ttggttgctg agagaactgc ttcaccgcct tcctcaggta
     6661 atcggctctt acggaagtga cgttgtgatc cttcaacgag agcttctctc gacaattcca
     6721 actcttgagt ttcttaccaa ggcgcccaga attctggatg tcgatgatgc aatatggctg
     6781 catcgtaggg ggatcgccgc gaattcgatt gctcgtcgtg tcgaccatat cgtgtgtggc
     6841 aatcaatacc tggccgatta cttcggtcag ttcggtaggc cgactacgat cattccgacg
     6901 ggcgtcgata ctcttcgttt tttacccaga agggaacgca gggagaatag agtgatcggc
     6961 tggtccggca cgtcgggtgg ctaccgcttt ctttatgaca tagaaatccc gctgtcccac
     7021 ctgttcagag atcaccccga atggaaattg aggatcgttt cggaccgtcc tcccgagttc
     7081 aaggtcattc cagccgaaaa ggttgagttc attcgctgga cagagaccaa tgaggtcgaa
     7141 acgattgccg gaatggatat cggtataatg cctcttgccg atgatctatg gtcgaggggg
     7201 aagtgtagct acaaaatgtt gctttatatg gcctgtggct tgccggtagt ggtttccgag
     7261 tatggtatga accgcgatgt tctagcccga ggcttcatcg gatatggtgc ggtggacgac
     7321 gagggctggt atgagtcact tgcggctttg gtcaaagatc cggaagcccg tgtgcgtgca
     7381 gggcaaaatg gaagagatat tatcgaaagg cactattctc tcgatgtcgt ttgtgacctg
     7441 tgggcaatgg tcattggctc ggtagcgcct ggaaagggaa cgaggtgatt tgatgtgtgg
     7501 tatcgctggc ttctggaata ttacgggaac tctcctaggg gataatgcac gtgtggcccg
     7561 gcagatggct gccgccatac atcatcgtgg accggatgag tcggggattt ggtacgaggc
     7621 tccgcgcgcc ccgatacttg tccatgcgcg tcttgcggtt ctggagcttt cccccgcagg
     7681 cagccagccc atgcactccg actgtggtcg gtatgtgctg atttacaatg gcgagatata
     7741 caaccatctg gctcttaggg ctcgcctgag cgaggcaggt gtaacacatt cctggcgtgg
     7801 cggatcggac accgaaacct tattggcatg ctttgcccaa tggggcgtgg agagtaccct
     7861 caagctcacc gtgggtatgt tcgctctggc cttgtgggat cgacaggaga aaacaattac
     7921 tctggctcgt gatcgaatgg gcgaaaagcc tctctattgg ggctggcaga atggcgtgct
     7981 tttttttgca tccgaactga aggccctgaa agaacatccg ctttttaggg gggatattga
     8041 tagagatgcc ttggccctat ttctacgata tggatatgtc ccggctccct atagtattta
     8101 taaaggtatc gggaagctaa gggcagggag ctatttggtc ctatcggaac gttcattgaa
     8161 cgagacctgc gaacctgctg cctattggag tgcgaacgcc gcgatagaag aggctctctc
     8221 caatccattc cagggaacgg atgccgaggc agttgatctt ctggaaagtc agttgagaac
     8281 tagcatatcg gatcagatgg tctccgatgt tcctttggga gcttttctga gcggtggggt
     8341 ggatagtagt acggtcgtgg cgctgatgca gcagcagtcc tcacgcccta tacggacatt
     8401 ctctattggc tttgatgagc ctgggtatga tgaggctgtt tacgcaaagg cggttgcaga
     8461 acatatcggt accgatcata cagagctcta tgtcaattca aaagatgctc tagatgtaat
     8521 tccatctttg ccaaagatct actgtgagcc atttggtgac agttcgcaga taccaacctt
     8581 gattgtcagt ggattggcca ggcagcaagt tactgttgcg ctgagtggag atggtggtga
     8641 tgaactgttt ggcggctaca atccctatca gtttactccc cgtgtctggc gcatgctgga
     8701 gcgatttcct cattccatgc ggcgtttcgc atcggctttt gctcaggact tacctttacc
     8761 tgaaaagttg gggaagctgc gtgacgtatt tgcttcgagg acggccgagg agcttttcta
     8821 tcgattgaac agtcactgga gaaatcacga atacccggtc ataggtgcac agggtcacac
     8881 ggcactattg gatactccag aaagatggcc aagggtggac tcgttccagc actggatgat
     8941 ggccatggat gttcagggct acatgcctga cgacattctg gtgaaggtag acagagcagc
     9001 gatggctaac agcctggaaa cccgggttcc actgatagac catcgcgtgt tcgaactggc
     9061 ttggcgcatg ccgttgcata tgaagattcg caatggaaaa ggaaagtggt tgcttcgtga
     9121 agtcttgtat aggcatgttt cgcgagagct catagagcgg cccaagaagg ggttttccgt
     9181 tcctgtctcc gattggctga gaggccctct gaaagagtgg gctgaatcgc tgcttgatga
     9241 gcgacgcctt cagcaagaag gttatttgga tagtaggttg atacgcagga tctggaacga
     9301 ccacttggcg ggaaggcggg accactcaag acgcctctgg agtgttctca tgttccaagc
     9361 gtggttggag tcatgaccac agtaaaagtt atccacgtga tagccggcct gaagaaagtc
     9421 ggcggtgctg agctaatgct caagcggttg atcgagactc aaaatggcgg cagtgaattc
     9481 gaacattcga ttatttcact gtctgatctt ggtgagtttg ggcagggtct gattgaggct
     9541 ggtatatctg ttgatgtgct tggcatgacc tcgatgaggg atatgcctcg ggtgctcctt
     9601 cgtctgatat ggatatttcg tgaaaggagg cccgatatag tccaaacatg gatgtatcat
     9661 tccgatcttc ttggtggatt ggctgccaga atggccggta ttggcgggat tatatgggga
     9721 gtccgcacga ccgaccttca agagggcggc aagagtacga ctgttctggt acggaaggtg
     9781 tgtgcctggc tatccggatt cttgccaaaa tatattgttt gcgccgccga ggcctcaagg
     9841 cgctcacaca ttgctgttgg ctacaacgca agcaggatgc tggtcattcc caacggtttc
     9901 gatctcactc gcctgcaggc gaccgacgaa cagcggtccg ctatcaggag tggagctggc
     9961 atagaggcca gcgatatcgt gattggcagt ctaggcagat tccacccggt aaaggatcat
    10021 gcttcctttg tcgcagctgc cggtctgctg gctccacgtt attcgcgctt gaagtttctt
    10081 ttggtgggac gggagctgct gtcttccaat gctgaattgc agcgcctcat cgaagctacc
    10141 gggtatgcgg agcgcttcat cctgcttgga gagcggcaag atgtagcttc gtgtctgaaa
    10201 gccatggata tcttctgtct gcattctcgc acggagggat tccccaatgt tcttggtgaa
    10261 gccatggcca tgggattgcc ttgtattacc actgatgtgg gggatgcggc ttatttgcta
    10321 ggtaacgacg gtgtcgtcgt tccggccttg gatccgaatg cgctcggcaa aggtatcgag
    10381 gatctcattg cgttggatgt cgagggtcgt cgggcacttg gtgaggctgc aagacagagg
    10441 atttactcga acttcacgat ggccagtgcc tctcagcgtt tcatgagtct gtatcgcgac
    10501 attcttgtaa aaagaaaagc ttgacgagct tgaaggttat gtttgtaaaa cgcatcgagg
    10561 agtttgcatg aagccggtat tgttgatgat agtgaacgac ccggctttct tcatgtctca
    10621 ccgtttaccg gtcgctgtcg gagcccagca ggctggtttc caggtacata tcgccacacg
    10681 ccctggtgac gcggtgaaaa agatcgtctc ccaaggtttt ctgcatcatg aattgccact
    10741 gtccagaagc ggaaaaaatc ctttttcaga gctgtatctg ctgacttatg tctggcgcct
    10801 tttgtggaga ttgcggcctg atgttttaca cctggttacc atcaagccgg taatctatgg
    10861 cggtatcgcc gcacgcttgg cgccggtgaa gggggtcgtg gcggccgttt ccggacttgg
    10921 tttcgttttc atggccaagg gtctgaaggc ctgcgcgttt cgtgcctgtg tcgcctggct
    10981 ttatcggcgg gctttgggca agaaaaagtt gcgtgtcatc tttcaaaatc ccgatgatcg
    11041 ggacgcgctg atcggcctgg gtgcgatcac ttttgaaaag tccgtcctga ttcgcggttc
    11101 gggggtcgac ctgacgcagt accagccggc cccggaaagc cccgaaacgc cggtggtcac
    11161 attggcggct cggttgctca gggacaaggg tgtgctggaa ttcgtcgaag cggcaaatat
    11221 ccttaggcag cgcagcgtac cggcacattt tcaactggtc ggagacctgg atcctgggaa
    11281 ccccaccagt atcgagccgt ccgaactaga gcgttggcgt agcgagggca cgatcgagtg
    11341 tcttggttat cgccaggata tcgcgtctgt cttcgcccgt tcgcatatag tcgttctgcc
    11401 ttcttatcgt gaagggcttc ctaaggtcct ggtcgaggcg tcggcgtgcg ggcgtgcggt
    11461 tgtgacgacc gatgtaccgg gttgccggga tgcaatccag gctgatgtta cgggcctgct
    11521 cgtgccggtg cgtgattctg ctgctctggc ggatgccatt cagcgtctta ttgagtcgcc
    11581 agagttgcgc aagaagatgg gagctgctgg gcgagcgttg gcagagcgtg attttgcgat
    11641 cgaaagtatc gtccaacagc acctggatat ttatcgggca ctagggagcg gagcttgacg
    11701 cgacataatg tattggtaac gggggcaacc ggattcatcg gggccgcgct ggtcaatagt
    11761 ctctgttctt ccgggcagta caaggtttgg gccgggtgcc gccgaagagg tggggcctgg
    11821 cctcgagggg ttactccctt gcttctgggt gaactgggca gttctgttgt ctgggatgcg
    11881 gagtcggcca tcgatacggt ggttcattgc gctgcccgcg tgcatgtcat gagcgagacg
    11941 gcctccgatc cgctcgtcga gttccgcaag gccaacgttc aggggacact tgatctggcc
    12001 cgggaggccg tatcccgggg agtgcggcgt ttcatcttta tcagctcgat aaaggtcaat
    12061 ggcgagggaa ctgagcccgg tcggccctat actgcggaca gtccgcccaa tcctgtcgat
    12121 ccctatggcg tttccaaacg ggaagcggag caagcgctat tggatctggc ggaagagacc
    12181 ggactggagg ttgtgatcat ccgaccggtc ctggtttatg gtccaggggt caaggccaac
    12241 gtacagacca tgatgcgctg gctgaagcgc ggcgtgcccc tccctctagg ggctattcat
    12301 aatcggcgta gtctggtgtc gctggataat cttgtcgacc tcatcatcac ctgcatcgag
    12361 catccggctg cggtcgggca agttttcctc gtaagcgacg gtgaggatct ttccaccacc
    12421 gagttgctgc ggagaatggg acgcgccttg ggcgctcccg ctcgcctgct gcctgttccc
    12481 gccagttgga tcggtgcagc ggcgaaagtt ctgaacaggc aggcatttgc tcgccgcctg
    12541 tgcggctcat tgcaagtcga tatcatgaaa acccgccaag tgcttggctg gactccgccg
    12601 gtaggggtgg accaggcatt ggagaaaacc gcccgctctt tcctggatcg ccaatgatga
    12661 atctatggtt gttgttgccg gcggttgccg ctctttccct gctgttgacc gccggcttgc
    12721 gccgttacgc gattgcccgc agcttgatcg atgtacccaa tgctcgaagt tctcaccagg
    12781 tgccgacgcc tagggggggc ggtgtcgcta tcgtgctctc cttcctgctg gcagtgctgc
    12841 ttgcggcgat attgggcgcg gtgaaaccgg atctggccac tggtatcctg ggggcgggta
    12901 ttgggatcgc attgctgggc ttcctcgacg atcatggcca tatcgctgcc cgttggcgtt
    12961 tgctcggcca tttcgccggt gcttgctggc tgctgtactg gcttggaggt ttgcctgcat
    13021 tggccttttt cggccttgtg gtcgatctgg ggtgggttgg acacatagct gcggcgttct
    13081 atctggtatg gatgctcaat ctctacaatt tcatggacgg tatcgatggg attgccagcg
    13141 ttgaggctgt gtgtgtgtgc gttggcgctg ccctgcttgt cgtggtcagt ggtgtaggtt
    13201 ccgatgaggc atcccagggc gtttggttgg cggcgctgct ggcagccgcg gtgacggggt
    13261 tccttttctg gaatttccct cctgcgcgga tcttcatggg ggatgctggt agcggtttct
    13321 tgggggttat cattggtggc ctttccctgc aagccgcatg ggtctcgccg cagctcttct
    13381 ggggttggct catcctgctg ggggtattca ttgttgatgc caccctgacc ttgttgcgcc
    13441 ggttgctgcg gggagacaag gtttatgagg cgcatcgcag tcacgcatac cagtatgctt
    13501 ctcgtcatta cgggcgtcat cttcctgtta ccctggcagt gggaggcatc aacatcttct
    13561 ggttgttgcc actggcactg cttgtcgcag cggggaagat cgacggcatg cttgctttgt
    13621 tgattggcta cctgcctctg gcgttcctgg cgctccgttt taaggccgga gtcctggaat
    13681 cacgcgctgc ttgaatgtcc cggttggctg gaggcgcggg acgattttcc gggaacgtat
    13741 aatatgaaag gaatgtgcaa gcgatgatca gtagtgacta ctgccgatag cgtgatttgg
    13801 gttccggaat ggtatttgcc agaccaagga aatggactgc ccggacttgt tcgggtggat
    13861 gcgggtgcag tgaagggggc tgattaatag gatgttggat aacttgcgtg gacgcctcct
    13921 gggattgccg cgccgccaga agcgcattct tcaggttgcg acggacatcg gcctggtgtg
    13981 gctttcattg tggctggctt tcctggtgcg tctcggcacc gaagacatga tcgatccgtt
    14041 cggggatcac gcctggctgt tcatagcggc gcctctaacc gccatcccgc tcttcatccg
    14101 cttcggcatg taccgggcgg tgatgcgcta cctgggcaac gacgccctta tcgcgatcgc
    14161 caaggccgtc accatttccg cgctggtcct gtcgttgctg gtctactggt accgctcccc
    14221 gccggcggtg gtgccccgtt ccctggtgtt caactactgg tggttgagca tgctgctgat
    14281 cggcggcttg cgtctggcca tgcgccagta tttcatgggc gactggtact ctgctgtgca
    14341 gtcggtacca tttctcaacc gccaggatgg cctgcccagg gtggctatct atggcgcggg
    14401 ggcggccggc aaccagttgg ttgcggcatt gcgtctcggt cgggcgatgc gtccggtggc
    14461 gttcatcgat gatgacaagc agatcgccaa ccgggtcatc gccggtctgc gggtctatac
    14521 cgccaagcat atccgccaga tgatcgacga gacgggcgcg caggaggttc tcctggcgat
    14581 tccttccgcc actcgggccc ggcgccgaga gattctcgag tccctggagc cgttcccgct
    14641 gcacgtgcgc agcatgcccg gcttcatgga cctggccagc ggccgggtca aggtggacga
    14701 cctgcaggag gtggacatcg ctgacctgct gggg
//
