LOCUS       AF498420               16349 bp    DNA     linear   BCT 13-JUN-2002
DEFINITION  Pseudomonas aeruginosa serotype 09 putative O-antigen biosynthesis
            gene cluster, partial sequence.
ACCESSION   AF498420 AC104739
VERSION     AF498420.1  GI:20560134
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V.
  TITLE     Genetic variation at the O-antigen biosynthetic locus in
            Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 184 (13), 3614-3622 (2002)
   PUBMED   12057956
REFERENCE   2  (bases 1 to 16349)
  AUTHORS   Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V.,
            Ivey,R.G., Zhou,Y., Kaul,R., Clendinning,J.B. and Olson,M.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2002) Genome Center, University of Washington,
            Box 352145, Seattle, WA 98105-2145, USA
COMMENT     On May 13, 2002 this sequence version replaced gi:17975268.
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /serotype="09"
                     /db_xref="taxon:287"
     CDS             <1..104
                     /note="ORF_1; C-terminal coding region of rpsA"
                     /codon_start=3
                     /transl_table=11
                     /product="RpsA"
                     /protein_id="AAM27867.1"
                     /db_xref="GI:20560135"
                     /translation="EKDAMKELRKQEVESAGPTTIGDLIRAQMENQG"
     CDS             242..526
                     /note="ORF_2; himD/ihfB"
                     /codon_start=1
                     /transl_table=11
                     /product="HimD"
                     /protein_id="AAM27868.1"
                     /db_xref="GI:20560136"
                     /translation="MTKSELIERIVTHQGQLSAKDVELAIKTMLEQMSQALATGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNEPE"
     CDS             581..862
                     /note="ORF_3; hypothetical orfA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27869.1"
                     /db_xref="GI:20560137"
                     /translation="MAIGVLLVALFMIVAVLENQQTTHLELFGLSSPEFPVVLYLALG
                     FILGGVIGLLVSVPILARLHIQLRSTRDDLSRLRNGMDSRQLASGQPEK"
     CDS             1062..2108
                     /note="ORF_4; wzz; similar to chain length determinant
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzz"
                     /product="Wzz"
                     /protein_id="AAM27870.1"
                     /db_xref="GI:20560138"
                     /translation="MTENRYSRQDADGEIDLLALVQGLWAGKWIIVGTAAVVTLLAVV
                     YAVLKEPVYESKITLLPPPLSAVAGFNIGRTRSEGLAPFTVSDVYRVFTRNLEADENR
                     RAFFRLYYLPTLDESDRKGPQDGLYRRFNEIFTIKVPDKQQPGYTLIIERKNPAEAAD
                     WVQKYVQMVAKQSIDEMLQNARRELDVKARNMRQQVDILKESAKERRADRITQLKEAL
                     AIAKALNLVKPPLVEGQSVKQLSSIMDGSLTYMRGTEAISAEIKALESRVSDDPFIPQ
                     LRGLEERYKLYENMSPEVQRIAVFRQDGAVETPDVPVKPNKVMIVFLGGVLGFVLGMM
                     IALFLHVLRKSRVS"
     CDS             2235..3485
                     /note="ORF_5; potential multiple membrane spanning
                     domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AAM27871.1"
                     /db_xref="GI:20560139"
                     /translation="MILARLSRLLNVGLRAVTLISKFALLFFLARYLTPTELGLYGLL
                     VAVVAYALYFVGFDFYAYSTREMLKSPEAKRGRMLRDQLVLTAILYLIFIPCLLSIFI
                     FNLLPWSFVWWFLALLVLEHWNQEVMRLLIALSAPTLAGWTLFFRSAAWALIVVFVMA
                     FDPSLRNLHFVLLGWVMGDALALVISLRSLRRMIPLDWHMPVDWLWIWKGIRVALPLL
                     ISTLAVRGLLTLDRYWVQQISGLEVLGAYVLFMGMAAALLAFLDAGVFTFIYPGMISR
                     FQCGEADSFKTLGRRLGLQTIVVCLVFSGFALFLVGPVIAWLGKDFYELQLRMFPWVL
                     AAIVLYALSMIAHYALYAQGHDRPIIFSHILGFLAFFPVVFWGGEILQILTVPVGLCV
                     AFSLILMWKLMAYWRLTPRQYRFF"
     CDS             3521..4519
                     /note="ORF_6; similar to Polysaccharide biosynthesis
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /protein_id="AAM27872.1"
                     /db_xref="GI:20560140"
                     /translation="MFKDKSILVTGGTGSFGNTFVPMTLAKYNPRRLIIYSRDEMKQW
                     EMAKKFQNDPRVRFFIGDVRDRERLYRALDGVDYVVHAAATKIVPTAEYNPFECVKTN
                     INGAMNLIDACIDKGVKRVVALSTDKASSPVNLYGATKLASDKLFVAGNSYAGGHDTR
                     FAVVRYGNVMGSRGSVIPFFMSIKDKGVLPITDERMTRFMISLEEGVELVWHAFEDME
                     GGEIYVKKIPSMKVTDLARVVAPDAKLEVVGIRPGEKLHEQMISAEDAYYTYEYPEHF
                     KILPSINNWATCEKRIKNGIKVPEGFVYASDNNTEWMSDEMLQDWIEQNREKIGSI"
     CDS             4523..5683
                     /note="ORF_7; similar to DegT/DnrJ/EryC1/StrS family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf4"
                     /protein_id="AAM27873.1"
                     /db_xref="GI:20560141"
                     /translation="MIPYGRQEITQADIDAVVEVLKSDFLTQGPMVPRFEQTVAEHVG
                     ARHAIAVNSATSALHIACLALGLGVGDWLWTSPVTFVASANCGLYCGAKVDFVDIDPA
                     TYNICPKALSEKLEVARKDGCVPKVLVVVHLCGQPCDMKAIRDLADQYGFKIIEDASH
                     AIGGRYLGEYIGNCRYSDITIFSFHPVKIITTAEGGMALTNDDGLASRMVLLRSHGIT
                     RVPEQMTHSPDGPWYYQQVDLGFNYRMTELQAALGLSQMSRLNEYVERRHKLAQRYNE
                     KLSNLPLNTPWQDPRSYSGLHLYVIRLKLSEIEKTHLEVFESLRKQGVGVNLHYIPVH
                     TQPFYERMGFSVGDFPEAEAYYREAISLPMYHTLSLEQQDKVVEALEQALLK"
     CDS             5761..6387
                     /note="ORF_8; similar to Cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /protein_id="AAM27874.1"
                     /db_xref="GI:20560142"
                     /translation="MIAWSIEAAIESGCFDKVMVSTDDAEIAAVARKYGADVPFMRPV
                     ELADDHAGTLPVIRQAIEGYLEKGVFAEQVCCIYATAPFVRPEDLYQGCTRLEESGAA
                     YAFSVTTFAFPIQRAIRLKEDGRVEMFQPAYMASRSQDLEEAYHDAGQFYWGRSEAWL
                     KEIPIFAGNAVPILLPRHRVQDIDTPEDWVRAEWLFKALLSEADVSSS"
     CDS             6384..7487
                     /note="ORF_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /protein_id="AAM27875.1"
                     /db_xref="GI:20560143"
                     /translation="MKVAFRVDASLDIGNGHVMRCLTLASYLRELGGESLFICRDHPG
                     NLISLISDKGFSVNILPVEPFTPTSEDGYESWLGATQERDSEVCKKILREFSPDWLVV
                     DHYGLDARWEISVKPFFKYLFVIDDLANRPHVCDVLLDQNLGRKVCDYLGLVPTGCRL
                     LVGVRYALLRPEFPKLRDFSLQRRRQPCVNKLLVTMGGVDQPNATGLVLHALLGSSLP
                     EDCQVSVIMGANAPWLEKVKLEAARLPWQVEVSVNVDDMAQRMASSDLIIGAAGSTSW
                     ERCCLGVPSILVVLAENQRIAASALRDCGAVKLLDIKNDFEENLRMELRTLLTDLESL
                     KGLSAGSAAVTDGGGGELIAELMKMYSHAGGVA"
     CDS             7484..8038
                     /note="ORF_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /protein_id="AAM27876.1"
                     /db_xref="GI:20560144"
                     /translation="MSHDLGILRAIKAEELEVMLMWRNAPSVRANMYTRHEISWDEHL
                     AWWERTKSRKDQAYFMYEYKGVSSGIVAFTGIDSANRNSSWAFYASTEAPKGTGSRME
                     FLALDYAFGDMGLHKLYCEVLAFNAPVIKLHQKYGFKAEGVFREHHMVDGAYVDIHRL
                     GILATEWNDKRSEIKEKLIALSRG"
     CDS             8042..9097
                     /note="ORF_11; similar to NeuB family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /protein_id="AAM27877.1"
                     /db_xref="GI:20560145"
                     /translation="MTSSIVIAGREIGCGFPPYIIAELSANHNGKIENALDIVSAAKA
                     SGADAVKLQTYKPETITLRSDAEEFKITEGLWRGRTLYDLYEEAHMPWEWHKPIFEHA
                     ARLGITIFSSPFDSTAVDLLEDLNTPAYKIASFEAVDLPLIRYAASTGKPMIISTGMA
                     NAEEIEEAIVAARDAGCASLAILRCVSGYPAPPEDYNLLTIPDMIRRFNLVTGLSDHT
                     IDNTTAITSVALGASIIEKHFTLNRDGGGPDDSFSLEPAELAALCRDSKTAWSALGKI
                     DYGRKSSEIGNVKFRRSLYFVKDMRKGQLITQDSIRSVRPGFGVAPKYLESIVGRTVS
                     DDVRSGTAVSWDLISAE"
     CDS             9121..10068
                     /note="ORF_12"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /protein_id="AAM27878.1"
                     /db_xref="GI:20560146"
                     /translation="MHTYYIQYTTDFHLIAALGAVGDLSAGKIYLIGPRTKVVDRVLG
                     MEGVNLEFHQTDPRSPSISWKRVIKLLKCSRSNDESTIVSPFVFPFYTFLMLLEKGER
                     VGRIIRTDEGVGSYASMRHYYVSLRFERPNSTRLYCAALAFLKKSAAWSVSFLRVCTE
                     SYIFNPDLSINKAKTQAIQAVVERLGRAKGLNGRVIYVSQPGISQCFNSAQGYAEFLR
                     LTGNALGEMPVVKRHPADNFDYSMHGFEVVEGLPLETYQINTATVFGFSSTALLMAKI
                     IGGCEQVYYINSGAAAAYYMGLSDFNKRLFKFNLTALSP"
     CDS             10082..11236
                     /note="ORF_13; potential multiple membrane spanning
                     domains."
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AAM27879.1"
                     /db_xref="GI:20560147"
                     /translation="MAYLFYCFLTLPFALFSVFLRRDFQFLLFLFFVAICVFVFGFSY
                     KVGSDWYNYFSYFDSGCTGFEFDVGFEYLCSGFNWLGIGYWFFSFSIKAFYIVTLAWF
                     LWRLGVFPLLAFFSCILVSFVFLDNMLRQQVAFGFILVSVTCVRDRKILAFVCLCLAS
                     SFHFSAVFFIPFYFLYISFSFRNLFAFVAVLSFFIGLSGNSIVDYLVNFLAGADGGYL
                     GRALAYLEFDGYPVTLGHYFRFLILLMFVFLDLVIRGRQLSEQDSRIWRLISSGVILM
                     LFYEMVFYEFSVFWMRIREYFLVFFMVSPLFLFRVFFWREARIFSALYVVYPLAVFWG
                     VITGLPVYDDLYRDYRNYIVYLAVGDDGFDSIRDLAAQDYWDRWRRGELR"
     CDS             11252..12217
                     /note="ORF_14; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /protein_id="AAM27880.1"
                     /db_xref="GI:20560148"
                     /translation="MNVLVTGGAGFIGSKVVAELLKRGMNVRISSRKMLSIVGVDCVK
                     VDLLNSEVDLDFLVSDCTVILNCLGELQDESKMHALHVEATERLIIAAKRSARQSGLP
                     VHWVQLSSVGAYGPVLEKANSERIVTEETASAPVGVYECTKTIADELVMSSVENGVFS
                     YSILRPSNVYGSGMPNGSLRQWARLIKAHCFFYVGPPGSVSTYVHVDDVVEALLLCAF
                     EERAKNQIFNISNDCTQESLVEAMAGLQGVSPPFLRFPEALARMIAFVFSGIKMFPLT
                     SSRIDSLVARTRYSCNKLNHILGYVPSRDITKEISEVILDKEGRR"
     CDS             12274..13398
                     /note="ORF_15; similar to Glycosyl transferases group 1"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr1"
                     /product="glycosyltransferase"
                     /protein_id="AAM27881.1"
                     /db_xref="GI:20560149"
                     /translation="MTFFVETQLRAQISALAHAGAEITIVASEKELSKEIENCRYASI
                     VIPRKISPLSDLLALYKLWRLFRKERFDIVHSTTPKAGLLCALAGKLAFVPIRVHTFT
                     GQPWVGLKGLKYLLSKGSDKLITLLNTHCYADSISQRKFIIDSGVADGDDISVIGEGS
                     LAGIDLKRFAASRYSLNERVSLKASLGIGGASKTLLFVGRLTQDKGVLELLAAFESLV
                     GNHDVFLMLLGPHEMSEEELLAGFSSDLKERLILLGFSDEPEKFMAIADMLLLPSYRE
                     GFGTVVIEAAAMGVPTIGSDIYGLSDAIVNGETGLLVPVKNSQALAAAIDQLLGDERL
                     CKELGEKARIRVEKEFSSQRISNLLIGEYTRLLEKDRGDS"
     CDS             13395..14354
                     /note="ORF_16; similar to NAD dependent
                     epimerase/dehydratase family"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAM27882.1"
                     /gene="orf13"
                     /db_xref="GI:20560150"
                     /translation="MSRILVTGASGFVGRALSEQLQRLGHEVVAAARSTSSRIPSSVR
                     SVLTGDLCPDTDWSEALQAVDIVIHAAARVHVMYETLADPLQEFRKVNVEATLNLARQ
                     AASKGVRRFIFISSIKVNGEATEDGRPYRADDEPRPIDPYGLSKLEAERGLLDLAART
                     GIEVVIIRPVLVYGPGVKANFLNMMKVINKKIPLPFLLVDNRRSLVALTNLVDLITSC
                     LEHPSAANQVFLVSDDEDLSTSELLKRMGIALGAPARLLPVPVFLMKFIARILGKGAL
                     SQRLLGSLQVDISKTKELLAWHPPLNVDKALKETARHFLDSKE"
     CDS             14351..15376
                     /note="ORF_17; similar to Glycosyl transferase group 4"
                     /codon_start=1
                     /transl_table=11
                     /gene="gtr2"
                     /product="glycosyltransferase"
                     /protein_id="AAM27883.1"
                     /db_xref="GI:20560151"
                     /translation="MNLLIILPVVGALAFLLTAILRRYALANSVIDVPNDRSSHSMPT
                     PRGGGASIVISFLLAILLLGFLGVADWSFVIALFGAGLGIAIIGFLDDHGHIAARWRL
                     LGHFAAASWILYWLGGFPPLLLLGTTLDMGWFGHILAAIYLVWMLNLYNFMDGIDGIA
                     GIEAITVCLGGVLLLFCLGKGLDNEGVWVLAVLASAVLGFLIWNFPPAKIFMGDAGSG
                     FLGVVLGALSVKAAWVAPELFWSWLILLGVFVVDATFTLLRRLLRGDRIYEAHRSHAY
                     QYASRYFGKHLPVTLGVFFINVLWLFPFALAVGLGMVDALLGVFVAYLPLILIAVRFR
                     AGSLETV"
     CDS             15507..>16349
                     /note="ORF_18; similar to N terminal coding region of
                     Polysaccharide biosynthesis protein WpbM"
                     /codon_start=1
                     /gene="wbpM"
                     /transl_table=11
                     /protein_id="AAM27884.1"
                     /db_xref="GI:20560152"
                     /translation="MIDGLRVRLLGLPRRYKRILQVLADVVLVWLSLWLAFLVRLGTE
                     DMISPFGGHAWLFIAAPLVAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSL
                     LVYWYRSPPAVVPRSLVFNYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDG
                     LPRVAIYGAGAAGNQLVAALRLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMI
                     DETGAQEVLLAIPSATRARRREILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDI
                     ADLLG"
BASE COUNT     3545 a   3483 c   4534 g   4783 t
ORIGIN
        1 acgagaagga cgcaatgaaa gaactgcgta agcaggaagt agaaagcgct ggtccgacca
       61 ccatcggtga tctgatccgt gctcagatgg agaatcaggg ctaagtctct gatccatcat
      121 gaaaaagggc ggnctangnc gncctttttt cgttttcccc ttcttggacc tgttcaaaga
      181 ctgatcagca tgctaaaaga gacctgagct gatctagccg cttgaaaaag aagggaaaac
      241 catgaccaag tcggagttga tcgaacggat cgttacccat caggggcaac tgtccgcgaa
      301 ggatgtcgag ttggcaatca agaccatgct ggagcaaatg tcccaggccc tggcgaccgg
      361 ggaccggatc gagatccgtg gtttcggcag cttttccttg cactaccgcg ccccgcgcgt
      421 cggtcgcaac cccaagaccg gggagtcggt acgcctcgac ggcaagttcg tgccgcactt
      481 caagccgggc aaggagttgc gggatcgggt caacgagccg gagtgatgta ccggccatta
      541 tgatactgga gtcttcatgc tttggatcag gcgtgccttg atggcgattg gagtgctgct
      601 tgtagctctt ttcatgatcg tggctgttct ggaaaaccag caaaccactc acctcgaact
      661 ctttggttta agctccccgg aattccctgt tgtcctctat ctcgccttgg gattcatcct
      721 gggtggtgtt atcggtttgc tggtcagtgt ccctattctc gctcgtctcc atatccagct
      781 caggtctacg agagatgatc tgtctcgcct tcgcaatggg atggattccc gacagcttgc
      841 ttctggacag cccgagaagt agagcttgtc caggtctggt caggagctgg catattccgc
      901 gcttgctcga taggcggagg gaactgttgg aagggtgtgc gatctgacat aggtcgtatc
      961 tggcgacttg taatatcgca aagggttggc acaatgtgcc agccctcggg gtcgtagggt
     1021 ttacctatgg cgaggcctga aacagatgaa acggacctgt cgtgactgaa aatcgctatt
     1081 cccgccaaga tgcagatggt gaaattgact tgctggcgtt ggtgcaaggg ctgtgggctg
     1141 gaaaatggat catagtggga actgcagccg tagtgacatt gctagcagtt gtatatgcag
     1201 ttttgaagga gccagtttat gaatccaaaa ttacgcttct tcctcctccg ctaagtgccg
     1261 tggcgggatt caatatcggg cgtacgcgtt ctgaagggtt ggcccctttt accgtttctg
     1321 atgtctatag ggttttcact cgcaacctgg aggctgacga gaatcggcgt gctttctttc
     1381 gcctgtatta tcttcctact cttgatgaaa gcgatcgcaa agggccgcag gatgggcttt
     1441 acagacgttt caacgaaata tttaccatca aagttcccga taagcagcag cctggctata
     1501 cgctgatcat tgaacgaaag aatccggcag aggccgctga ctgggttcag aaatatgtgc
     1561 agatggtggc gaagcagtct atcgatgaaa tgcttcagaa tgcccgacgc gaactagacg
     1621 tgaaagcaag aaacatgcgt cagcaggtag atattctcaa ggagagtgcc aaggaacgtc
     1681 gtgcggaccg aattactcag ctcaaggaag ctctggccat cgccaaggct ttgaatcttg
     1741 ttaagcctcc tcttgtggag ggacagtctg tcaagcagct ctcttctatc atggatggga
     1801 gtctgaccta catgcgcggt accgaagcga tttccgcgga aataaaagcg ttggagtctc
     1861 gtgtttctga tgatccattt attcctcagt tgagagggct ggaggagcga tacaaactct
     1921 atgagaacat gagcccggaa gtgcagcgta tagctgtatt ccgacaagat ggcgctgtgg
     1981 aaacacccga tgtacctgtt aagccaaata aagtaatgat tgtttttttg ggtggcgtgc
     2041 tggggttcgt attggggatg atgattgctc tctttttgca tgtactgagg aaaagtaggg
     2101 tcagttgact ctgttcttga tgtggcatta gggaagaaaa gggcaggaag taaattcact
     2161 ctaatggcct gctgcgatga aggagcgctc atattgagat catttttatt ttgcgggcgt
     2221 gtgggcggta gggcatgatc cttgctcgcc tttcgcgtct gctgaatgtg gggttgaggg
     2281 ctgtaaccct tatctcgaag ttcgctcttt tgtttttcct tgctcgttac ctcacgccta
     2341 cggagctcgg gctgtatgga cttttagtgg cagtcgtcgc gtatgctttg tattttgtcg
     2401 gcttcgattt ttatgcatat agcacgcgtg aaatgctgaa gtcgccagag gcaaagcgtg
     2461 ggcgtatgtt acgagatcag ctggttttga cggctattct ctatctgatt ttcattccat
     2521 gtcttttgtc gattttcatt tttaaccttc taccgtggtc ctttgtctgg tggtttctgg
     2581 cgttgctggt gctggaacat tggaatcaag aggttatgcg attgttgatt gccttgtctg
     2641 cgccaactct tgccggatgg acattgttct tccggtcagc ggcttgggca ttgattgtag
     2701 tttttgtcat ggctttcgat ccgtcgctaa ggaaccttca ctttgttttg ttaggctggg
     2761 tcatgggtga cgctttagcg cttgttatct cattaagaag tttgcggcgc atgattccct
     2821 tggattggca catgccggtg gactggttgt ggatatggaa agggatcagg gtggcactgc
     2881 ctctcttgat ttcgactctt gcagtgcgtg gccttcttac cttggatcga tactgggtgc
     2941 agcaaatttc cgggctggag gttcttggtg cttacgtatt gttcatgggt atggctgctg
     3001 ccttgttggc ttttctagac gcaggggttt ttacattcat atatccgggg atgattagta
     3061 ggtttcagtg tggagaagcg gattccttca agaccttggg gcgacgcctg ggcctccaga
     3121 caatagttgt atgcctggta tttagtggct ttgctctatt tcttgtcggt cccgtcatag
     3181 cttggcttgg caaggatttc tatgagctgc agctgcgaat gtttccttgg gtcttggcag
     3241 ctatagtgct atatgcattg agcatgattg ctcattatgc gctttatgcg caggggcatg
     3301 atcgccctat aattttcagt catattctcg gtttcctggc tttcttccct gttgttttct
     3361 ggggaggaga gatcttgcag atactcaccg tacccgtagg gttgtgtgta gcgttttccc
     3421 ttatactaat gtggaaactg atggcctatt ggcgcctgac gcctaggcag tatcggtttt
     3481 tttgaatcag ccttcatgga gcctagataa aggggatcat atgtttaaag ataagtcgat
     3541 tctagtgacg ggtggaacag ggtcctttgg aaatacattc gttccaatga cattggctaa
     3601 atataacccg cggcgcctga ttatttactc tcgtgatgag atgaaacaat gggagatggc
     3661 caagaaattt cagaatgacc ctcgtgttcg cttctttatt ggtgatgttc gcgatcgcga
     3721 gcggctatat cgtgccttgg atggcgttga ctatgttgtt catgccgctg cgaccaagat
     3781 tgttccgact gctgagtaca atccttttga gtgtgtgaag actaatatca atggggcgat
     3841 gaacctcatt gatgcgtgca tcgacaaggg cgtcaagaga gttgtagcac tgtctacaga
     3901 caaggccagt agccctgtta atctgtatgg ggccaccaaa cttgcctcgg acaagttgtt
     3961 tgtcgcgggc aactcatatg cgggagggca tgatactcgc tttgcggtgg tacgttacgg
     4021 aaacgtcatg ggctctcgcg gctctgtgat tcccttcttt atgtcgatca aggacaaggg
     4081 cgtcttgcct atcactgatg agcggatgac ccggttcatg atttctttgg aggagggcgt
     4141 cgagttagtg tggcatgcct ttgaggacat ggaaggtggt gagatctatg tcaagaaaat
     4201 tccttcgatg aaagtgactg acctcgctcg tgtggtcgct ccggatgcaa agctggaggt
     4261 agtgggaatc cgtccgggtg agaagctgca tgagcagatg attagtgcgg aagatgctta
     4321 ctacacgtat gagtatcctg agcacttcaa gattcttcca tctataaata actgggctac
     4381 gtgcgaaaag cgtataaaga atggcataaa ggttccggaa ggttttgttt acgcgagtga
     4441 caataataca gaatggatga gtgatgaaat gctgcaggat tggatcgaac agaatcgcga
     4501 aaagatcggg agcatctgag tcatgattcc ttatggccgg caggagatta cccaggcgga
     4561 tatcgacgcg gtagtcgagg ttcttaaatc tgatttcctt actcagggtc ctatggttcc
     4621 ccgttttgaa cagaccgttg cggaacacgt aggggcgcga cacgctattg cggtgaatag
     4681 cgcgacttct gctttgcata ttgcttgtct agctttgggc ctgggtgttg gggattggtt
     4741 atggacgtcg ccggtcacct ttgtggcatc ggccaattgc ggcctttatt gtggggcaaa
     4801 agttgatttt gtcgacatcg atccggcaac ttataatatc tgcccaaagg ctctttctga
     4861 aaagcttgaa gttgcaagga aagatgggtg tgtgccgaaa gtcttggttg ttgtccatct
     4921 ctgcgggcaa ccatgtgata tgaaggccat ccgtgacttg gcagaccaat atggcttcaa
     4981 gatcatcgag gatgcttccc atgccattgg tgggcgctat ctaggagagt atattggcaa
     5041 ctgccgctat agcgatataa ccatattcag ttttcatcct gtgaaaatta ttacaaccgc
     5101 agaaggcggg atggctttga ccaatgatga tggtttggcc agtcgcatgg ttttgttgcg
     5161 tagtcatggc attacacgtg tacccgaaca gatgactcat tcccctgacg gcccttggta
     5221 ttatcagcaa gtggacctgg gcttcaacta tcgcatgacg gagttgcaag cggcgttagg
     5281 tttaagtcag atgtctcgtt tgaatgagta tgtcgagcgt cgccataaat tggctcaacg
     5341 ttataacgaa aaattgtcca atttgccgct gaatacacct tggcaggatc cgaggagcta
     5401 ctctggatta catctctatg tcatacgtct caagctaagc gagatagaga aaactcacct
     5461 tgaagtgttc gagtctttga ggaagcaggg tgtcggggtg aatttgcatt acattcctgt
     5521 acatactcag cctttttatg agcgcatggg gttttctgtt ggagattttc cggaagctga
     5581 agcttactat cgcgaagcga ttagtttgcc tatgtaccat accttgtctc tggagcagca
     5641 ggataaggtt gtagaagcct tggaacaggc gcttcttaaa tgaaaatcgc catcatcccg
     5701 gctcgtggag gtagtaagag gatcccgcga aagaatataa gggatttttg cgggaaacca
     5761 atgattgcct ggtccatcga ggcggccatt gagagtggct gctttgacaa ggtaatggtt
     5821 tccactgacg atgctgagat agctgctgtt gcgcgtaaat atggggctga tgtacccttc
     5881 atgcgaccgg tagagttagc tgatgatcat gcaggaacat tgcctgtgat tcgtcaagct
     5941 attgagggct atctggagaa aggtgtcttc gctgagcagg tatgctgtat atacgctacc
     6001 gctccttttg tacgtcctga agacctgtat caaggctgta cccggcttga ggagtcaggc
     6061 gcggcatatg ctttctcggt tactacattc gcctttccca ttcagcgtgc aatacgcttg
     6121 aaagaggatg gtcgtgtaga aatgttccag cctgcttata tggcgtctcg atctcaagat
     6181 ctggaggagg cctatcatga tgctgggcag ttttactggg ggaggagcga ggcatggctg
     6241 aaggaaatcc ctatctttgc cggaaacgcc gttcccattc tccttcctcg tcatagggtc
     6301 caagacatcg atactcctga agattgggta agggcagagt ggttattcaa ggccctgctc
     6361 tctgaggcgg atgtatcttc atcatgaagg tggctttccg ggtagacgca tccttggata
     6421 taggaaatgg tcatgtcatg cgctgcctga cattggccag ctatctgagg gaactgggag
     6481 gggagtctct attcatctgc agagatcatc caggaaacct aattagcctt atcagtgaca
     6541 aaggattttc ggtcaatatc ctccctgttg agccattcac acctacttcc gaagatggat
     6601 atgagagttg gttaggtgct acacaagaac gggactccga ggtttgcaag aaaattctga
     6661 gggagttctc tcctgattgg cttgtcgtgg accattatgg gttggatgct cgctgggaaa
     6721 tttctgtaaa acccttcttc aaatacttat ttgttattga tgatcttgcc aacaggcctc
     6781 atgtttgtga tgtgttgctt gatcagaatc tagggcgaaa ggtctgcgac tatttgggac
     6841 tagtccctac ggggtgtagg ttgctggtcg gtgttcgata tgccttgctt cgtccggagt
     6901 ttcctaaact aagagatttc agccttcagc gccgtaggca accgtgtgta aataagctgt
     6961 tggtgacgat gggcggggtt gatcaaccga atgcaaccgg attggttctt catgcgctac
     7021 taggcagttc tctgcctgaa gattgccaag ttagcgtcat aatgggcgcc aatgctccat
     7081 ggttggaaaa ggtaaagcta gaagctgccc gtcttccatg gcaggttgag gtatctgtca
     7141 atgtcgacga catggcacaa cgtatggcta gtagcgattt gatcattggt gcggccggaa
     7201 gtacctcctg ggagcgctgt tgcctggggg tcccttccat attggtggtt ctggcggaaa
     7261 accaacggat cgccgcgagc gcgcttcgag attgtggagc ggtaaagctc cttgatatca
     7321 aaaatgattt tgaagaaaac ttgaggatgg aactacgtac tctcttgacg gatctggagt
     7381 cgctcaaggg gctgtctgca ggctccgctg cggtaacaga cggtggtggg ggagagttga
     7441 tagctgaact catgaaaatg tactcacatg ctggaggagt cgcatgagcc atgatctcgg
     7501 tatcctaagg gcaatcaagg cagaagagtt ggaagtaatg cttatgtggc gaaatgcccc
     7561 aagtgtaaga gccaacatgt acacgcggca tgaaatcagc tgggatgagc atcttgcctg
     7621 gtgggaaaga accaagtcca gaaaagatca ggcatatttt atgtatgaat acaagggagt
     7681 ttccagcggg atcgtggctt ttaccggcat tgactcggcc aataggaact cttcttgggc
     7741 tttctatgca tcgacagagg ctcccaaggg aacaggaagt cggatggagt ttcttgcgct
     7801 agactacgct tttggcgata tgggacttca taagctgtat tgtgaagttc ttgcatttaa
     7861 tgctcccgtt atcaagctcc atcagaagta tggattcaaa gcggaaggtg tcttcagaga
     7921 gcaccacatg gttgacggtg cgtacgtgga tattcatcgt ttgggcattc ttgcgacaga
     7981 gtggaatgac aagcgctctg aaattaagga aaaactcata gcgctttcga gaggctgagt
     8041 catgacgtct agtattgtta ttgcaggtcg ggaaattggt tgcgggtttc ctccatatat
     8101 tattgcggaa ctttctgcga atcataatgg taaaattgaa aatgcgcttg atatagtgag
     8161 cgcagcaaaa gcgtcagggg ctgatgcggt caagctccaa acctataagc ccgaaaccat
     8221 tactcttcgg tctgatgccg aagaatttaa aatcacggag gggttgtggc gtggtaggac
     8281 tctctatgat ctttatgaag aagcacatat gccttgggag tggcataagc ccatatttga
     8341 gcatgctgca cgactaggga ttaccatatt tagttcaccg ttcgactcca ctgccgtgga
     8401 tttgctcgag gatctaaata cacctgccta caagattgct tcattcgaag ccgttgatct
     8461 tccacttatt cgatatgccg caagtacggg taagccgatg atcatttcaa cagggatggc
     8521 caacgctgaa gaaatagagg aagctatagt cgcagcccgt gatgctggtt gtgcttcatt
     8581 ggctatcctg cgctgcgtga gtggttatcc cgcgccacct gaagattata atcttctcac
     8641 tattccggat atgatacgcc gtttcaactt ggttaccggg ctttccgacc atactatcga
     8701 taacactaca gcaattacca gcgttgccct aggggcgtct atcatcgaaa aacactttac
     8761 tctgaataga gatggtggcg gtcccgatga ttcattctct cttgagccgg ccgaactcgc
     8821 tgctctttgc agagatagca agactgcatg gtctgctttg ggtaagattg actatggcag
     8881 gaagtcaagt gagataggaa acgtcaagtt tcgtcggtct ctttattttg tcaaggatat
     8941 gaggaaggga cagctcatca ctcaggattc gataaggagc gtcaggccag gtttcggtgt
     9001 ggcacctaaa tatttggaat cgattgttgg aagaacagta agtgatgatg ttcgatctgg
     9061 aacagctgta agctgggatc tgatttcagc tgagtaaagg gcggtcttag gcgcttgggt
     9121 atgcacacct attatattca gtatacaacg gattttcatc tgatcgctgc gttgggagcg
     9181 gtgggggacc tgagtgctgg aaagatctat ttaataggtc ctcgaacgaa ggttgttgat
     9241 cgtgtacttg gtatggaggg ggtgaatcta gagtttcatc agacggaccc cagatcaccc
     9301 tctatatcct ggaagagggt catcaaactc ttgaagtgtt ctcggtcaaa tgatgagagt
     9361 actatcgtct ctcccttcgt cttcccgttc tatacctttc ttatgcttct cgagaaaggc
     9421 gagcgggtag gccggatcat aagaaccgat gaaggcgttg gtagctatgc ttccatgagg
     9481 cactattatg tttcgttgcg atttgaaagg ccgaacagta cgcgtctata ctgcgccgct
     9541 ctggccttcc tgaaaaaaag tgcggcttgg agcgtctctt ttctgagagt ttgcacagag
     9601 tcatatattt tcaatcctga tctttccatt aataaggcta aaacacaagc cattcaggca
     9661 gttgttgaga ggcttggcag ggcgaaaggg ttgaatggtc gtgtcatata tgtaagtcaa
     9721 ccgggtattt ctcagtgttt caatagtgct cagggctatg ctgaatttct gagattgact
     9781 ggaaacgctc ttggcgagat gcccgtggtc aagaggcatc cggctgataa ttttgattat
     9841 tctatgcatg ggtttgaggt agttgaaggc ctgccgttgg agacttatca gataaatacc
     9901 gctacggttt ttggtttttc atctactgca cttcttatgg caaaaatcat cggggggtgc
     9961 gagcaagtgt attatataaa ttccggagct gctgctgctt actatatggg tttgtctgac
    10021 tttaataaaa ggcttttcaa gttcaatttg acggcgttga gtccctaggg cactgattag
    10081 tatggcttac ttgttctatt gtttcttaac tcttccgttt gcactgttca gtgtgttttt
    10141 gagaagagat tttcagtttc tgctgttttt attttttgtg gctatttgtg ttttcgtgtt
    10201 tgggttttct tataaggttg ggtcggattg gtataactat ttttcttatt ttgattctgg
    10261 ttgtacgggt tttgagtttg atgtcggttt tgaatatctt tgctctggct tcaactggtt
    10321 gggtattgga tattggtttt ttagtttttc catcaaggct ttctatatag ttaccttggc
    10381 ttggtttctt tggcggcttg gcgtttttcc cctgctggca ttcttttcat gtattctggt
    10441 ttcttttgtt tttctggata atatgcttcg gcaacaggtt gcctttggat ttatccttgt
    10501 ttctgttact tgtgtcaggg atagaaagat ccttgctttt gtttgtctat gcctggcttc
    10561 tagctttcat ttttcggccg tatttttcat tcctttctat tttctgtaca tcagtttctc
    10621 ttttcgcaac ctgtttgctt ttgtggctgt gctctccttc tttatcggat tgagcgggaa
    10681 tagcattgta gattatctgg ttaatttctt ggctggagca gacggtggat atcttgggcg
    10741 ggcgttggca tatcttgaat ttgacggata cccggtcacg cttggccact attttcgatt
    10801 tctgattttg ttaatgttcg tgtttttaga tctcgttatc agagggcgac aactgagcga
    10861 acaggactct agaatttgga ggctcatatc ttctggtgtg atccttatgc tcttctatga
    10921 aatggtgttt tatgagttta gtgttttctg gatgagaatc agggagtatt ttctcgtctt
    10981 ctttatggtt tcacccctct ttttatttcg tgtcttcttc tggcgtgagg cgcgaatttt
    11041 ctcagctctc tacgtcgtct atcctttggc agtgttctgg ggagtaatca cagggcttcc
    11101 tgtttacgac gatctttata gagattatag aaattatatt gtctatctcg ctgttggcga
    11161 tgatgggttc gactctattc gagacttggc tgcccaggac tattgggata gatggcgccg
    11221 aggcgagttg agatagtcat tgggatatag catgaacgtt ttggttactg gtggtgctgg
    11281 cttcattgga agcaaggtgg ttgcggagct gctgaagcgg ggcatgaatg tccgaatctc
    11341 ctcaaggaag atgctatcca tcgttggggt tgattgtgtg aaagtcgacc tcctcaacag
    11401 tgaggtcgat ctcgacttcc tggtgtccga ctgtacggtt attctcaact gcctcggaga
    11461 gcttcaggat gagagcaaga tgcatgctct tcacgtcgag gcaacagagc gactgatcat
    11521 tgctgcgaaa agaagtgctc gccaatctgg tttgccagtg cattgggtcc aactaagcag
    11581 tgtgggtgca tacggtcctg ttctggaaaa ggccaactcc gagcgtattg tcacggagga
    11641 aaccgcttct gctccggtag gagtttatga atgcaccaag acgattgccg atgagctggt
    11701 gatgtcttcc gtcgaaaatg gtgttttcag ctattctatt ttgcggccgt ccaatgtgta
    11761 tggttcagga atgccaaacg ggtcgttgcg tcaatgggcg cgattgatca aggcgcactg
    11821 ttttttctat gtggggcccc caggatcagt ttctacctat gtacatgttg acgatgttgt
    11881 cgaggcactc ctgctctgcg catttgagga aagagcgaag aatcagatat tcaatatttc
    11941 caatgattgc acacaggaga gtctagtgga ggcgatggca ggattgcagg gagtctcccc
    12001 accttttttg cgcttcccgg aagcgttggc gagaatgatc gccttcgttt tttcggggat
    12061 aaagatgttt cctctgacat catctcgtat agattctctg gtggcgcgca ctcgttactc
    12121 atgcaataaa ttgaatcata tattaggtta tgtgcctagt cgtgatataa caaaagaaat
    12181 atctgaggtt attctcgata aggaaggccg gcgttgagct attttaataa aaaaaagggg
    12241 gttgagggcg tgcgcatcgc gagggtctct actgtgacct tttttgttga gacccaattg
    12301 agagctcaaa tttccgcact tgcccatgcg ggtgccgaga tcacgattgt tgctagtgaa
    12361 aaagagcttt ctaaagagat tgaaaattgc cgttatgctt ccattgtaat tccaagaaaa
    12421 atttctccgc ttagcgatct cctggctttg tataaactct ggcgtctttt tcgaaaagag
    12481 cggtttgaca tcgttcactc taccactcca aaggcaggct tgttatgtgc gctagcaggc
    12541 aagcttgcct tcgtacccat ccgtgtgcat acttttacgg ggcagccttg ggtgggttta
    12601 aaaggtctga aatatcttct ctccaagggc agtgataagc ttataacgct tctcaatact
    12661 cattgctatg cggatagtat ttcgcagcgc aagttcatca tcgatagcgg tgttgcagat
    12721 ggtgatgata tctcggttat tggtgaaggg tcgcttgccg gaattgatct gaaacgtttc
    12781 gcggcttcgc gttatagctt gaatgaaaga gtttctctga aagcttccct gggtatcgga
    12841 ggagcctcga agactctgct ttttgttggg cgtcttactc aagacaaggg ggttttggag
    12901 ttattggcgg cttttgagag cttagtcgga aatcatgatg tttttcttat gctccttgga
    12961 ccgcatgaaa tgagcgaaga agagcttttg gccggttttt ctagtgatct caaggagcga
    13021 cttattcttt tggggttttc cgatgagccg gaaaagttca tggctatagc ggatatgctg
    13081 ctcctgccta gctacaggga gggttttggt actgtggtaa tcgaggccgc ggccatgggg
    13141 gtgccgacta tcggttcgga tatctatggt ctgagcgatg ccattgtcaa tggtgagaca
    13201 ggcctgcttg tgccagtgaa aaactctcaa gcgttggctg cggcaatcga tcagctatta
    13261 ggtgatgagc gactctgcaa ggaactggga gaaaaggctc ggattcgagt ggagaaagaa
    13321 ttttccagtc aacgtattag caatcttttg attggtgaat atacacggtt gctggagaaa
    13381 gacaggggag actcatgagc agaatcctgg tgacaggggc ctcaggtttt gttggaaggg
    13441 ctttgtcgga gcagttgcag cgtctggggc atgaagtcgt tgccgctgca cgaagcacat
    13501 cctctcgcat tccttcttct gtacggtctg tattgactgg tgacctgtgt cccgatactg
    13561 actggtccga ggccttgcag gcggtagata tcgttattca tgccgcggca cgcgtccatg
    13621 tgatgtatga aacgcttgcc gatccacttc aggagttccg caaggtgaat gtggaggcaa
    13681 ccttgaactt ggcgcgacag gctgcgagca agggggtacg gcgcttcata tttatcagtt
    13741 caataaaggt caatggagaa gctacagagg atgggcgtcc atacagagcc gatgacgaac
    13801 cccggccgat tgacccctat ggcctgtcta agctagaggc ggagagaggt cttctggatc
    13861 tggcagcaag aactggaatc gaggtggtta taattcgccc ggtcttggtt tatggtccag
    13921 gcgtcaaagc caatttcctc aacatgatga aggtcattaa taagaagatt ccgcttccct
    13981 tccttctggt tgacaatcga agaagcttgg tggcgttgac caatcttgtc gatttgatca
    14041 cctcctgcct tgagcatcct tcggccgcga atcaagtatt tttggtaagc gacgatgaag
    14101 atctttccac gagtgaactt ttaaaacgca tggggattgc attgggtgcg cctgctcggc
    14161 tgcttcctgt gcctgttttt cttatgaagt ttatagcaag aatacttggt aagggtgcgt
    14221 tgtcccaacg attgcttgga tctttgcagg ttgatataag caagacaaaa gaacttttgg
    14281 cttggcatcc acctttgaac gtcgataagg ctttgaagga gacggcgcgt cactttctgg
    14341 attctaaaga atgaatttac taattattct tcctgtagta ggtgccttgg ctttcctgct
    14401 aacggcaatt ttgcggagat atgcgctggc caatagcgtg attgatgtac ccaatgaccg
    14461 tagctcccac tccatgccta ctccacgggg aggaggcgcc tctatagtga taagtttttt
    14521 gctggcaata ttgcttctgg gtttcttagg ggttgccgat tggagttttg tgattgcatt
    14581 attcggggca ggccttggta tcgctattat tggcttcctt gacgatcatg gacatatcgc
    14641 tgcccgctgg aggcttctcg ggcattttgc agctgcgtcc tggatacttt attggctggg
    14701 cggttttcct ccgctgcttc ttctaggtac cacccttgat atgggatggt ttggtcatat
    14761 attggccgcc atatacttgg tttggatgct taacctctat aacttcatgg atggaatcga
    14821 tggaattgcc ggtatagaag ccataaccgt ttgccttggc ggtgtactgc ttcttttctg
    14881 cctgggaaag gggctggata atgaaggggt ttgggttctt gccgtgcttg caagtgccgt
    14941 tctgggcttt ttgatttgga acttccctcc ggcaaagata ttcatgggag atgcgggaag
    15001 cggattccta ggtgtcgttc tgggggcgct ttccgtcaag gcagcatggg tggccccgga
    15061 gcttttttgg agttggctga ttcttctggg tgtttttgtc gtcgatgcaa catttacttt
    15121 attacgaaga cttcttcgtg gggatcgcat ttatgaagcc catcggagcc atgcgtatca
    15181 gtacgcatct cgatacttcg gaaaacattt gcctgtcacc ttgggagttt tcttcataaa
    15241 tgttctctgg ctctttccat ttgctctggc agttgggttg ggaatggtcg atgcgctact
    15301 aggtgtgttt gttgcatact tgcctctgat cttgattgcc gtgaggtttc gtgccggatc
    15361 gctggagacg gtttgactcc tggaaccttt cgcacccctc ttgagcctaa cccaggcctg
    15421 gtgtgacgtt tcagtgcttg tctctatctg gatttgctaa tctggttgcg tttgtgagct
    15481 gtttagaagg gagtggggag agctggatga tagatggttt gcgtgttcga ctgctggggc
    15541 tgccacgtcg ttataaaagg atcttgcagg tccttgccga cgtagtgctg gtctggcttt
    15601 ccctttggtt ggcattcttg gtgcggttgg gtacggaaga catgatcagc cccttcggcg
    15661 gccatgcctg gctgttcatc gccgccccgt tggtggccat tcccctgttc atccgcttcg
    15721 gcatgtaccg ggcggtgatg cgctacctgg gcaacgacgc ccttatcgcg atcgccaagg
    15781 ccgtcaccat ttccgcgctg gtcctgtcgt tgctggtcta ctggtaccgc tccccgccgg
    15841 cggtggtgcc gcgttccctg gtgttcaact actggtggtt gagcatgctg ctgatcggcg
    15901 gcttgcgtct ggccatgcgc cagtatttca tgggagactg gtactctgct gtgcagtcgg
    15961 taccatttct caaccgccag gatggcctgc ccagggtggc tatctatggc gcgggggcgg
    16021 ccggcaacca gttggttgcg gcattgcgtc tcggtcgggc gatgcgtccg gtggcgttca
    16081 tcgatgatga caagcagatc gccaaccggg tcatcgccgg tctgcgggtc tataccgcca
    16141 agcatatccg ccagatgatc gacgagacgg gcgcgcagga ggttctcctg gcgattcctt
    16201 ccgccactcg ggcccggcgc cgagagattc tcgagtccct ggagccgttc ccgctgcacg
    16261 tgcgcagcat gcccggcttc atggacctgg ccagcggccg ggtcaaggtg gacgacctgc
    16321 aggaggtgga catcgctgac ctgctgggg
//
