LOCUS       HQ388392               12901 bp    DNA     linear   BCT 05-APR-2011
DEFINITION  Salmonella enterica strain G1459 serogroup O16 O-antigen gene
            cluster, complete sequence.
ACCESSION   HQ388392
VERSION     HQ388392.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Li,Y., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Liu,B., Wang,L. and Knirel,Y.A.
  TITLE     Structural and genetic relationships of two pairs of closely
            related O-antigens of Escherichia coli and Salmonella enterica : E.
            coli O11/S. enterica O16 and E. coli O21/S. enterica O38
  JOURNAL   FEMS Immunol. Med. Microbiol. 61 (3), 258-268 (2011)
   PUBMED   21205000
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   Li,Y., Perepelov,A.V., Guo,D., Shevelev,S.D., Senchenkova,S.N.,
            Shahskov,A.S., Liu,B., Wang,L. and Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin,
            Tianjin 300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1459"
                     /db_xref="taxon:28901"
                     /note="serogroup: O16"
     gene            105..1340
                     /gene="wzx"
     CDS             105..1340
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADV17636.1"
                     /translation="MLKNIIYLLAVQGGNYIFPLITLPYLVRVLEPTGYGIYGYSFAL
                     IQYFILLVDYGFNYSAPKAISLNRYNNDNISSVFWNIVAIKIIIASIGFIVLSFIFMV
                     NFINYPSSIVFLAYLTVLGNIAYPVWLFQGLEKMAGIAISMLISRIISVFLTFLLVKD
                     VNDLAMAVLIQSSITITAGVISIFFLISLRKINWIMPSFTKMKELIIDGWHYFISSAA
                     ISLYTTSATIILGIFTGPATVGYFIAADKIRLALQGIIGPVTQAVFPRVSYIIKDNPE
                     NGITIAKKVFKWQFTIMFFLSALLYFLSPYIIHILYGETYHMSLDILKILAFTPAVVT
                     VSNYIAILVMIPSDMKKEFMRIITTSGVVGLICITILTYLYSATGTAVGIISTEIIIM
                     VRFYLVVKRNKKINSFQLL"
     misc_feature    105..12690
                     /note="O-antigen gene cluster"
     gene            1350..2075
                     /gene="wdaD"
     CDS             1350..2075
                     /gene="wdaD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaD"
                     /protein_id="ADV17637.1"
                     /translation="MKKAILSVITVVYNGEKYIEDTIKSVLSQPCINDIEYIIIDGAS
                     TDNTLNIINKYKQHLALVISEKDNGIYDAMNKGIEKATGKYIGFINADDFYNEGALCH
                     AIEIMKANEYDIIYGNMFMVDVETLEVKGVRRPKHWKLNIDMNLSHPATFVKSSVHKI
                     NLFDLNYKIAADYDLMLKLKKNKCTFHYIDKNISSMRNGGVSVRNIKLANKEAKIIRI
                     KRNGILFSLVASMYLAMRRIIKA"
     gene            2072..3304
                     /gene="wzy"
     CDS             2072..3304
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADV17638.1"
                     /translation="MNNQDLKIGTAFISMIYFPLGFLVSLIEVINGYRWGNFLFAFIL
                     GLLAFSLIPYSDWDLTRHYETYLSYNNTSFSEVWEQSDNRYLVINTIIYLFNTFAIKK
                     EFLPFFAVFISYLFYLNVFSKFIREKKPNILIRSIYLYILLTVIPFFAIASSLRQYLA
                     FSIILYIIITYCSKQDRRTFLISFPLVVMAIGIHPSVILLIALFLFSRFIRLNRIVVL
                     LIFFILVLNFNGYISIQLFKLFKPLLMNTGFYYPEYMDAEVIHMNNQLLSTNEFILNK
                     LILPSIFYLLIPVFLIFYKKSNSKQEKQLKNYCALLLLTICLLSLSPDLFYRFSIFWT
                     LFYSYIYFTYDSERMSLPAKQCYLVIIIVASCVINLAQANMGKKIIYNSWGSFFYQPS
                     LFVFVKEIKTTDYINVSQ"
     gene            3306..4373
                     /gene="wdaE"
     CDS             3306..4373
                     /gene="wdaE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaE"
                     /protein_id="ADV17639.1"
                     /translation="MTIAIFKLYANKIGGTEKAVQSLVSILNQNRYANECYAAVDVKE
                     VQNSDDKCITLYNYESFFKKNKLKFYINYYLFLFHNIQKKQLIISTDIATSSLLMLAN
                     LMRFTRLNIICWEHYPFEKNSRIWQKIFKYLIFLNKKSVIVCNSTEEKKKFSFHNKIE
                     VIPNSIKRTLYNQLYIEKEKLDLIYIGRIVKDKGVERLIDAIISFPGDKKHTLHIVGG
                     GDELEYLKKKYAQIKEIIFYGPIINIEKFLEKNDVFVSGSYFECFPVTILEAMSHGLP
                     IISMDTSGGTRSVISDSGGGYVCENKIEFHEKLSIFENSTVRAEHGKAGRSYVNKNYS
                     DSIIGSKWIRLIENELTMKSE"
     gene            4398..5420
                     /gene="gne"
     CDS             4398..5420
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADV17640.1"
                     /translation="MTILVTGGAGYIGSHTVLKLLQHRYKVISLDNYTNSSPISLDRV
                     ENISSGELIRYQGDVRDRIILKEIFYKHDIDAVIHLAGLKSVNESIRRPLEYYDNNTL
                     GTLILLEEMHQAGVYNLIFSSSATVYGNPQIVPISETHNTGDVINPYGRSKYMAENIL
                     KDYYHANNTANITILRYFNPVGAHESGLIGEDPNDIPNNLFPYITQVAIGKLTSLKIF
                     GNDYETKDGTGVRDYIHVVDLAEGHLSALKGLTNKGDLQIYNLGTGNGHSVMEVINAF
                     QEVTGTKIPFTFSSRRQGDVAECWSDASLAREKLQWAAKYSLKKMVTDAWRWQKMNPN
                     GYKNIE"
     gene            5489..6490
                     /gene="wdaF"
     CDS             5489..6490
                     /gene="wdaF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaF"
                     /protein_id="ADV17641.1"
                     /translation="MKILYDGIVRNLQSYGGITVYFEEITRRLEMHEYKFVNFSSLRN
                     DIYCDTNRRARLFERYRCFKTRSTDQECNIFHSTYYRFPKDNNFPVVTTVHDFTYELY
                     MRGIRKTIHSLQKNRAIQNSDSIICVSQNTAKDLLNFCDVDDKKIHVIYNGVSEAYKY
                     LNLEEEEFILFVGARAGYKNFELAVKAVAKLSKMKLKIVGGGTLSNSEIKLLTEYLDD
                     KYEYLGKITNEHLNILYNTAYCLIYPSEYEGFGIPLLEAMRTGCPVIAVNRSSIPEVV
                     GDSAILVDRVSIDSLFSALLRVPLNRNELRYKGLLQSQKFSWDKCFSETYDLYKSLC"
     gene            6509..7630
                     /gene="gmd"
     CDS             6509..7630
                     /gene="gmd"
                     /note="GDP-mannose-4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ADV17642.1"
                     /translation="MTKTALITGITGQDGSYLAEFLLEKGYTVHGIKRRSSSFNTERV
                     DHIYQDPHVNNPKFHLHYGDLTDTSNLTRILSEVKPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLEKKTKFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWMMLQQEYPEDFVIATGKQISVREFVRMAALEAGIELEF
                     SGIGVDEVGTVVKVTGDSEKYVRVGDEIVRVDSRYFRPAEVETLLGDPSKAKDKLGWV
                     PEISVEEMCREMVNSDLQKAKQYALLRENGFDVSISLEN"
     gene            7634..8596
                     /gene="fcl"
     CDS             7634..8596
                     /gene="fcl"
                     /note="GDP-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ADV17643.1"
                     /translation="MTKRVFVAGHNGMVGSALVRMLQQEQDIEIITRKRNELDLLSQQ
                     AVSSFFKSERIDQVYLAAAKVGGIYANNTYPADFIYQNLMIECNIINAAFHNGVQKLL
                     FLGSSCIYPRDTIQPIREDALLTGILESTNEPYAVAKIAGIKLCESYNRQYGCDYRSV
                     MPTNLYGPNDNFHAENAHVIPALLRRFHEASLDESESVIVWGSGNPMREFLYVDDMAA
                     ASVHVMNLPKEIYDKNTAPMLSHINVGTGVDCTIRELAETIASVVKFRGDIIFDESKP
                     DGTPRKLLDVSRLANMGWRWSTSLEEGLQLTYAWFLKNQEKFRK"
     gene            8606..9052
                     /gene="gmm"
     CDS             8606..9052
                     /gene="gmm"
                     /note="GDP-mannose mannosylhydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="ADV17644.1"
                     /translation="MLDYALFKTIVDNTPLISIDLIIYNAKSEVLLGKRNNPPAKAHY
                     FVPGGRIYKNEKITEAFQRICKAEIGVDIYLQSARFLGVFEHFYEDAYVGEDISTHYV
                     VLAYEIHMDLELNMLPLAQHNQYVFMPSELIASTSEVHFYTQRYFC"
     gene            9062..10456
                     /gene="manC"
     CDS             9062..10456
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADV17645.1"
                     /translation="MSNIILPVVMAGGAGSRLWPLSRALYPKQFLSLTSTNSMLQETI
                     IRLEGIEHEYPLFICNEEHRFIVAEQLRQLSIDHSGIILEPDGRNTGPAIALAAFHAL
                     NNGYDPVMLVLAADHEIQERKNFIDSIEIAKQFAENDKLVTFGIVPASPETGYGYIKV
                     GEKIGPDGYKVAAFVEKPHRLLAEEYINSGKYLWNSGMFMFKASVFLNELKKFRPDIY
                     SICETSLLKSESDLDFIRVNGKVFNCCPSESIDYAVMENTMQAVVVPLDAKWSDVGSW
                     TALWDISKKNENGNIIRGDVIAKDTDNCYLHSESRLIGTVGVKDLVIVETKDAVLVAH
                     KNNVQDVKNIVEQLKIQNRQEYLHHREVYRPWGSHDAVADGERFRVKHVTVLPGHAIS
                     KQVHYHRAEHWVIVSGTARVYIGDENFLLGENESTFIPVGTVHSIENPGKIPLEIIEV
                     QSGTYLHEDDVERS"
     gene            10559..11323
                     /gene="wdaG"
     CDS             10559..11323
                     /gene="wdaG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaG"
                     /protein_id="ADV17646.1"
                     /translation="MKVSIITATYNSQDTLKDTLLSVEQQDYPDIEYIIIDGGSSDQT
                     LQLIKKYSTRVKCLISEKDNGIYDALNKGVKLATGDIVGFIHSDDILASPQVISNIVN
                     KFKGNTADIVYGDLLFVDREKPDIIHRFWHSGEFKKSKLRYGWAPPHPAFYIRRELYK
                     KYGCFDLIFKIAADYDQMLRLLLVPYLQIIYVPEVFVKMRLGGESTKLNNALLSTKEI
                     IAIMRKHNINWQVAILTRKLSKLWQIFSKFKRSNIS"
     gene            11320..12690
                     /gene="manB"
     CDS             11320..12690
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADV17647.1"
                     /translation="MKKLPCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLG
                     GDVRLTSEALKMALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNEAARGSYRQISLRNAYIDHLL
                     GYISVNNLTPLKLVFNAGNGAAGPVIDAIEARLKALGASVEFIKIHNTPDGTFPNGIP
                     NPLLPECRDDTRKAVIEHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KHPGAKIIHDPRLTWNTEAVVTAAGGTPVMSKTGHAFIKERMRTEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKRQSLGELVRDRMAAFPASGEINSRLAEPAAAIAR
                     VESHFAEEAQAVDRTDGLSMSFPDWRFNLRSSNTEPVVRLNVESKANTELMEEKTQAI
                     LTLLRK"
BASE COUNT     4176 a   2007 c   2606 g   4112 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgtttatggt atagtaactg ttaacttaaa
       61 ataattaata tattgattgc atttttgcca aataaaggcc ttgtatgtta aaaaatataa
      121 tttatttact cgcagttcaa ggcgggaatt atatatttcc attaattaca ctaccatatc
      181 ttgttagagt tcttgagcca actggctatg gtatctatgg ttatagcttt gccttgatac
      241 aatactttat attgcttgtc gactacggtt tcaattattc tgcgcctaaa gcaatttctt
      301 tgaaccgtta taataacgat aatattagta gtgttttttg gaatatagta gcgataaaaa
      361 ttataattgc atcaatagga tttattgttc tctctttcat ttttatggtt aattttatta
      421 actatccatc ttcaattgtt tttctagctt atttaaccgt tcttggaaat atagcttatc
      481 cggtttggtt gtttcaggga ttagaaaaaa tggcaggtat agctattagt atgcttattt
      541 ctcggattat ctctgtcttt cttacatttc ttttagtaaa agatgtaaac gacttagcta
      601 tggcggtgct aatacaatct tctataacta taacggctgg ggttatatct atattttttc
      661 taatttcgct gcggaaaata aattggatta tgccttcctt tactaaaatg aaagagttaa
      721 ttattgatgg gtggcattat tttatatcat ctgcagctat aagtttatat accactagcg
      781 ccactataat attagggata tttacagggc ctgcgactgt aggctatttt atagctgcag
      841 ataaaattag attagcttta caaggtatta ttggtcctgt cacgcaagct gtattcccta
      901 gagtaagtta catcataaaa gataatcctg aaaatggtat aactatagct aaaaaagtgt
      961 ttaaatggca atttacaata atgttcttct tatcagcctt gctgtatttt ttatctccat
     1021 atattattca tattttatat ggcgaaacat atcatatgtc attagatatt cttaaaattc
     1081 tggcatttac gccagcagtg gttacagtta gtaattatat tgcaatatta gtcatgattc
     1141 cttcagatat gaaaaaggaa tttatgagaa ttattacaac tagtggtgtt gttgggttga
     1201 tatgtatcac gatattaact tatttatatt ctgcgacagg aacagccgta ggtattatat
     1261 ctactgaaat tatcataatg gtacgctttt atctggttgt aaaaagaaat aaaaaaatta
     1321 atagctttca attattgtag gtttgtatta tgaaaaaggc aattttatca gtaatcacag
     1381 ttgtttataa tggagaaaag tatattgagg acactatcaa aagtgtttta tctcagcctt
     1441 gtataaatga tattgaatat ataattattg atggtgcttc tacagataat acattgaata
     1501 ttattaataa atataaacaa cacttagcat tagtaatttc agaaaaggat aatggaatat
     1561 acgatgcaat gaacaagggg atagaaaaag caactggtaa atatattggg ttcataaatg
     1621 ccgatgactt ctataatgaa ggcgcgttat gccacgctat tgagattatg aaagctaatg
     1681 aatatgatat aatatatggg aacatgttta tggtcgatgt ggaaactttg gaggttaagg
     1741 gggtacgcag acctaaacac tggaagttaa atattgacat gaacctaagt caccccgcaa
     1801 cttttgtaaa aagcagtgtc cataaaataa atttatttga tcttaattat aaaattgcag
     1861 ctgattatga tcttatgtta aaactaaaaa agaacaaatg tacatttcat tatatagata
     1921 aaaatatatc atcaatgcga aatggcggag ttagtgtaag aaatattaaa ttagctaaca
     1981 aagaggctaa aataattagg ataaaaagaa atggaatttt attttccttg gtagctagta
     2041 tgtatcttgc tatgcgaagg ataataaaag catgaataac caagatttaa aaattggtac
     2101 tgcatttatt tcaatgattt attttccctt gggatttttg gtatcactta ttgaagtaat
     2161 taatggatat aggtggggaa actttctatt cgcatttatc ttgggtctat tagcattctc
     2221 actaataccg tatagtgatt gggatctaac aagacattat gaaacctatt taagttataa
     2281 taatacctct tttagtgaag tatgggaaca aagtgataat cgatatttag taataaatac
     2341 tataatttat ctatttaata ctttcgctat caaaaaggag tttttaccgt tctttgctgt
     2401 ttttatatct tatttgtttt acttaaatgt attttcgaaa tttattagag aaaaaaagcc
     2461 taatatttta atccgaagca tctatttata tattttatta acggtaatac ctttttttgc
     2521 tatagctagt tcactacgac aatatttagc cttcagtatt atattatata taataattac
     2581 ctactgtagt aagcaagata gacgtacttt ccttatatct ttcccactag ttgttatggc
     2641 aataggaata catccaagcg taattctgtt aatagcactg tttctattta gcagatttat
     2701 acgcctaaat cgaatagttg tgcttcttat ttttttcata ttagttctaa attttaatgg
     2761 ttatatttca atacaattat tcaagctatt taaaccatta ttaatgaata caggttttta
     2821 ttatccggaa tatatggatg cagaggttat tcatatgaat aaccaactat taagcacgaa
     2881 tgaatttata cttaataaat tgattttacc cagtatattt tatttgttaa tcccagtctt
     2941 tttgattttc tataaaaaat caaacagtaa acaggaaaag caactaaaaa actattgcgc
     3001 actactcctt ttgactatat gcctgttatc tttatcacct gatctttttt atcgattttc
     3061 aatattttgg acattattct atagctatat atatttcact tatgatagtg agagaatgag
     3121 tttgcctgcc aaacagtgct atcttgtaat tataattgtt gcatcatgtg tcataaactt
     3181 agctcaagct aatatgggca agaaaatcat atataattca tggggatcat ttttttacca
     3241 accttcacta ttcgtgtttg ttaaagaaat caaaacgact gactatataa atgtgagcca
     3301 ataagatgac gattgcaatt tttaaattgt atgcaaataa aataggcggt actgaaaaag
     3361 cagttcaaag cttagtaagc atcttaaacc aaaacaggta cgccaacgaa tgttatgcag
     3421 cagtggatgt taaagaagtt caaaacagtg atgataaatg tataacactt tataattacg
     3481 aaagtttttt taaaaaaaac aaattaaaat tttatattaa ctattatttg ttcttgtttc
     3541 ataacattca aaaaaaacag ttgattatat ctactgacat tgcaacatct agcttgctaa
     3601 tgcttgctaa tctaatgcga tttactagat taaatataat ttgctgggag cactatcctt
     3661 ttgaaaaaaa tagtagaata tggcaaaaaa tatttaaata cttaattttc ttgaataaaa
     3721 aatctgttat tgtctgtaac tcaaccgaag aaaaaaagaa attttccttt cataataaaa
     3781 tagaggttat accaaactct ataaaaagaa cattgtataa tcaactctat atagaaaaag
     3841 aaaagcttga tttaatttat ataggtagaa ttgttaaaga taagggagta gaaagactta
     3901 ttgatgccat aataagtttt cctggtgata aaaaacatac gttacatata gttggtggag
     3961 gtgatgagct agaatattta aagaaaaaat atgctcaaat aaaagaaata atattttatg
     4021 gccccattat taatatagag aaattcttag aaaagaatga tgtgttcgtt tcaggatcat
     4081 attttgaatg ctttccggtc acaattttag aggctatgtc tcatggttta ccgatcatat
     4141 cgatggatac atctggggga actagaagtg ttatctctga ctcaggtggc ggctatgtat
     4201 gtgaaaataa gatagagttt catgaaaaat tatccatatt tgaaaatagt acagtcaggg
     4261 cagagcatgg taaagcaggt cgaagttatg taaataagaa ctactccgat agtattattg
     4321 gttctaaatg gataagattg atagaaaatg aattaacaat gaaatctgaa taatcaatta
     4381 cttgataagg aggggatatg acgatattag taaccggagg ggctggttat attggttcac
     4441 atactgtatt aaaattactt cagcacagat ataaagtaat atcattagat aattatacta
     4501 attcctcgcc catatcctta gaccgtgtag aaaatattag ttccggtgag cttataagat
     4561 atcaggggga tgttagagat aggattatat taaaagaaat tttttataaa catgatattg
     4621 atgcagttat acatcttgct ggtttaaaat cagtaaatga atcaattaga cgtcctttag
     4681 aatattatga taacaataca ttgggaacgt taattttact tgaagaaatg catcaagctg
     4741 gcgtgtataa tttaattttt agttcatctg caactgtata tggtaaccct cagattgtgc
     4801 ctatatcaga gacgcataat actggagatg taataaatcc ttatggacgc tcaaaatata
     4861 tggcagagaa tatattaaaa gattactatc atgcaaataa tacagctaac attacaattt
     4921 taagatattt taatccagta ggcgctcacg aatcgggttt aattggtgaa gatccaaatg
     4981 atattcctaa taatcttttc ccatatataa cgcaggttgc aattggaaaa ttaacttcac
     5041 taaaaatttt tggtaatgat tatgaaacaa aagatggtac aggtgtccgt gattatatac
     5101 atgtagtaga tttagctgaa gggcatttat cagctttaaa gggattaact aataaaggag
     5161 atttacaaat atacaatctg ggtaccggga atggtcatag tgtaatggaa gtaataaatg
     5221 cttttcagga ggtaactggt actaagatac cttttacttt ttcttcaagg cgacagggag
     5281 acgtcgctga atgttggtct gatgcctcct tagcaaggga aaaactacag tgggctgcaa
     5341 aatattcctt aaaaaaaatg gttacagatg cgtggcgctg gcaaaaaatg aatcctaatg
     5401 gttataagaa tatagagtga gtatcattta gaattccttt cttcttacgt attccgaatt
     5461 aaggagaaga acctgagcat atatttatat gaagatatta tacgatggta ttgttagaaa
     5521 tttgcagtct tacggcggta taacggttta ttttgaagaa ataacacgta gattagaaat
     5581 gcatgaatat aaatttgtga atttttcctc tctacgaaat gatatttatt gcgatactaa
     5641 ccgtagggca agactatttg agcgctacag atgcttcaag acacgaagca ctgatcagga
     5701 atgcaatatt tttcattcta cttattatcg ctttcctaaa gataataatt ttcccgtcgt
     5761 tacaacagtc catgatttta catatgaact atacatgagg gggattcgta agactattca
     5821 tagtctgcaa aagaaccggg ctatacaaaa tagcgattca atcatttgtg tttcacaaaa
     5881 tactgctaaa gatttattga atttttgcga tgtagatgat aaaaaaattc atgttatata
     5941 caatggagtt tcagaagctt ataaatattt aaacttagag gaggaggagt ttatactttt
     6001 cgtcggagct agggcggggt ataaaaattt cgaactagct gtgaaagctg ttgctaagct
     6061 tagtaaaatg aaattaaaaa tagtaggtgg aggaacgtta agcaatagcg aaataaaatt
     6121 acttacagaa tatcttgatg ataaatatga gtatttagga aagattacga atgaacattt
     6181 gaatatacta tataatacag catattgctt aatatatccc tcagaatatg aaggatttgg
     6241 aataccgctg cttgaagcaa tgcgcacggg ttgtccagtt attgcagtaa atagatcatc
     6301 tatacctgaa gtagttggtg atagtgcgat acttgttgat agggtatcta ttgatagttt
     6361 attttcggcc ttgctaaggg tgcctttaaa cagaaacgag ctaagatata aaggattact
     6421 tcaatctcaa aaattttctt gggataagtg cttttccgaa acatatgatt tatataaatc
     6481 actttgttaa ttttatgagg gttttctagt gacaaaaaca gctttaatta caggtattac
     6541 tgggcaggac ggttcctatc tcgcggagtt tctattagaa aaaggttata ccgtacatgg
     6601 tattaaacgt cgatcatcct cttttaatac agagcgtgtt gatcatatct atcaggatcc
     6661 ccatgttaat aatccaaaat ttcatcttca ttatggagat ttgactgata cttctaactt
     6721 gacgcgtatc ttgtctgaag ttaaacctga tgaggtttat aatctcggtg caatgagcca
     6781 tgttgctgta tcttttgagt caccagaata tacagctgat gtagatgcta taggtacatt
     6841 acgcttgtta gaggctattc gtttcttagg attggaaaag aaaaccaaat tttatcaggc
     6901 ttcaacttct gaattgtatg gtttagttca agagatacct caaaaagagt ccacaccttt
     6961 ttatcctcgt tctccgtatg ctgttgctaa gctttacgca tattggatca ctgtaaatta
     7021 tcgtgaatca tatggtatgt atgcttgtaa tggtatttta tttaaccatg aatctccccg
     7081 ccgtggagaa acctttgtta ccagaaagat cactcgtgca attgcgaata tttctcaagg
     7141 tttagagtca tgtctatatc ttggaaatat ggactcatta cgggattggg gacacgctaa
     7201 agattacgta aggatgcaat ggatgatgct ccaacaggag tatccagaag attttgttat
     7261 cgcaacagga aaacaaatat ctgtacgcga gtttgttcgc atggctgctt tagaagcagg
     7321 catagagttg gagttcagtg gtataggtgt tgatgaagtt ggaacggtag tgaaggttac
     7381 gggagatagt gagaaatatg ttcgtgtagg cgatgagatt gttcgtgttg attctcgtta
     7441 tttcaggcct gctgaagtag aaactcttct tggagatcca tctaaagcga aagataagct
     7501 aggttgggtt ccagagattt ctgttgagga aatgtgtcga gaaatggtta atagtgatct
     7561 tcagaaagct aaacagtatg ctttgctgcg agaaaatggt tttgatgttt ctatttcatt
     7621 agagaattaa tttatgacga aaagagtttt cgttgctggc cataatggta tggttggttc
     7681 tgctttagta agaatgcttc aacaagagca agatattgag attatcacta gaaaacgtaa
     7741 tgagttagat cttctttctc aacaagctgt aagctctttt tttaaaagtg aaagaataga
     7801 tcaagtatat cttgctgcag caaaagtagg agggatttat gccaataata cctatccggc
     7861 tgactttatt taccagaatt taatgattga atgtaatatc attaacgcag cgtttcataa
     7921 tggtgttcag aaattattgt ttctaggatc tagttgtatt tacccgagag atacaataca
     7981 acctatacgt gaggatgcgt tattaactgg tattttggaa agtacaaatg aaccttatgc
     8041 tgtagccaaa attgcgggta ttaagttatg tgaatcatac aacagacaat atggctgtga
     8101 ttatcggagt gtaatgccta ctaatcttta tggaccaaat gataactttc atgctgaaaa
     8161 tgcacatgtt attcctgctt tacttagacg ctttcatgaa gccagtttgg acgaatctga
     8221 aagtgttata gtttggggga gtggtaaccc catgcgtgag tttctttatg ttgatgatat
     8281 ggcagcagca tcggttcatg tgatgaattt accaaaggaa atttatgata agaacacagc
     8341 gcctatgctt tctcatataa atgtcggcac tggagttgat tgtacgattc gtgaattagc
     8401 agaaactatc gcctcggtcg ttaagtttcg aggagatatt atttttgatg agtcgaaacc
     8461 ggatggtaca cctcgaaaat tattagatgt ttcacgctta gctaatatgg gatggaggtg
     8521 gtcgacatcg ttagaagaag ggcttcaatt aacttatgct tggttcttaa aaaaccaaga
     8581 aaaattcaga aaataggcac ttagtatgtt ggattatgcg ttatttaaaa cgatagtaga
     8641 taatactcca ttgatatcaa tagatcttat tatttataat gcaaaatctg aagtattgct
     8701 tggtaagagg aataatcccc cagctaaagc acactatttt gttcctggag gcagaatata
     8761 taaaaatgaa aaaatcacag aggcttttca acgtatttgc aaagcagaaa tcggtgttga
     8821 tatttatttg caatcagcaa gatttttagg tgtttttgaa catttttatg aagatgcata
     8881 tgttggagaa gatattagca cgcattatgt tgtattagct tatgaaatac atatggacct
     8941 agaattaaat atgttacctt tagcgcaaca taatcaatat gtttttatgc cttctgaatt
     9001 gatagcttcg acctctgaag tccattttta tacccagcgt tatttttgct aaaaggtcaa
     9061 aatgtctaat attatactcc ctgtagtcat ggctggtgga gcaggaagcc gactatggcc
     9121 actgtcaaga gctttatatc ccaaacaatt cctctcactt acatctacaa attcaatgct
     9181 tcaagaaaca ataatacgac ttgaaggaat agaacatgaa tatcccttgt ttatctgcaa
     9241 tgaagagcat cgatttatag ttgcagagca actccgtcaa ttgagtatag atcatagtgg
     9301 aattatcctt gaacctgatg ggcgtaatac aggtccggcc attgcgctag ctgcttttca
     9361 tgctcttaat aatggatatg atccagtaat gcttgttctt gcagcagatc atgagatcca
     9421 agaaagaaaa aattttatag attctataga aattgcaaaa caatttgctg aaaatgataa
     9481 gcttgtcact ttcggtatag ttccagcctc tcctgaaaca gggtatggat atataaaagt
     9541 tggtgaaaaa atagggcctg atggatataa agttgctgca tttgttgaaa aacctcatag
     9601 acttttggct gaagaataca taaatagcgg gaagtatctt tggaacagcg gaatgtttat
     9661 gtttaaagca tctgtctttc taaatgagtt aaagaaattt cgtccagata tttatagcat
     9721 ttgtgagact tcacttttaa agtcagagag cgatctcgat tttatacgag ttaatggcaa
     9781 agtattcaat tgttgcccat cagagtcaat agattatgct gttatggaaa atactatgca
     9841 agcggtagtt gttccgcttg atgcaaaatg gagcgatgta ggatcatgga cagctttatg
     9901 ggatattagt aagaaaaatg aaaatggaaa tattatacgc ggtgatgtta tagctaaaga
     9961 tacagataat tgttatttgc attcagaatc acgacttatt ggtaccgttg gagtcaaaga
    10021 tttagttata gttgaaacta aagacgcagt tttagtcgcg cataaaaaca acgtacaaga
    10081 tgttaaaaat atagttgaac aattaaaaat ccaaaatcga caagaatacc ttcatcatag
    10141 agaagtttac cgtccttggg gaagtcatga tgcagtggct gatggtgaac gatttagggt
    10201 taaacatgtt acggtattgc ccggtcatgc catatccaaa caagttcatt accatagagc
    10261 tgaacattgg gtaattgtga gtggaacagc tcgtgtatat ataggtgatg aaaatttttt
    10321 attgggagaa aatgagtcga catttattcc ggtcgggact gtacactcta ttgaaaatcc
    10381 gggtaaaatc ccactagaga ttattgaagt acaatcagga acgtatttgc atgaagatga
    10441 tgttgaaagg tcttaattac ttaatatatt atatattctg tagataatat tgctgttatt
    10501 aatattgtac attctttata ataactgtaa ttatttttag acagcattat ggattactat
    10561 gaaagtaagt attattacag ctacgtataa tagtcaagat acactaaaag atacattgct
    10621 atctgttgag caacaagatt atcctgatat agaatatata ataattgatg gaggttcctc
    10681 agatcaaact ttgcagctta ttaaaaaata ctccacaaga gttaaatgcc taatatcgga
    10741 gaaagataat ggtatttatg atgctttaaa taagggggtt aagcttgcga ccggtgatat
    10801 tgtcggattt atacattctg atgatatatt agcaagtcca caagttataa gtaatattgt
    10861 aaataagttc aagggaaaca ctgctgatat agtttatggt gatctccttt ttgttgatag
    10921 agaaaaacct gatattatac atagattttg gcatagcggg gaatttaaga aatctaagct
    10981 tagatatggt tgggctcctc cgcatcccgc cttttatata aggcgcgagt tatataaaaa
    11041 atatggctgc tttgatttga tttttaaaat tgcagctgat tatgatcaga tgcttcgttt
    11101 attactagta ccctacttac aaatcatcta tgtaccagaa gtctttgtta aaatgagact
    11161 tggtggagaa agtacgaagt taaataatgc gcttttaagt actaaagaaa taattgcgat
    11221 aatgcgtaag cataatataa attggcaagt agcaatattg acgagaaagt taagtaaact
    11281 ttggcaaatt ttttcgaaat ttaaaaggtc aaatatttca tgaaaaaact accttgtttc
    11341 aaggcctacg acattcgcgg caggctgggc gaagagctca atgaagatat cgcgtggcgg
    11401 attggccgcg cctacggcga atatttaaaa ccgaaaacca tcgtgctggg cggcgacgtg
    11461 cgcctgacca gcgaggcgct gaagatggcg ctggcaaaag ggttacagga cgcgggcgtc
    11521 gacgtgctgg atatcggtat gtccggcacc gaagagattt acttcgccac cttccacctc
    11581 ggcgtggacg gcggtattga ggtgaccgcc agccataacc cgatggacta caacggtatg
    11641 aagctggtgc gcgagggcgc ccgcccgata agcggcgaca ccggtctgcg cgacgttcag
    11701 cgtctggcag aagcgggcga cttcccgccg gtgaacgagg ccgcgcgcgg cagctacaga
    11761 caaatcagcc tgcgcaacgc ctacatcgac cacctgctcg gctatatcag tgttaacaat
    11821 ctcaccccgc tgaagctggt gtttaacgcc ggcaacggcg cggcggggcc ggtcattgac
    11881 gccatcgaag cgcgtctgaa ggcgctgggc gcgtcggtgg agttcatcaa aatccacaac
    11941 accccggacg gcaccttccc gaacggcatt cccaacccgt tgctgccgga gtgtcgcgac
    12001 gacacccgca aggccgttat tgaacacggg gcggacatgg gtatcgcctt tgacggcgac
    12061 ttcgaccgct gcttcctgtt cgatgagaaa gggcagttta tcgaggggta ctacatcgtc
    12121 gggctgctgg cggaagcatt tctggaaaaa cacccggggg cgaagattat ccacgacccg
    12181 cgcctgacct ggaacacgga ggcagtggtg acggcggcgg gcgggacgcc ggtgatgtca
    12241 aaaaccgggc acgcctttat caaggagcgg atgcgtacgg aggatgccat ttatggcggg
    12301 gaaatgagtg cgcatcacta tttccgtgat tttgcctact gcgacagcgg gatgatcccg
    12361 tggctgctgg tggcggagct ggtgtgcctg aagagacagt cgctgggtga gctggttcgc
    12421 gaccggatgg cggcgttccc ggcgagcggg gaaatcaaca gcaggctggc ggagccggcg
    12481 gcggccattg cgcgggtgga atcgcacttt gcggaagagg ctcaggcggt ggatcgcacg
    12541 gacggtctca gcatgtcatt cccggactgg cgtttcaacc tgcgctcgtc gaacaccgaa
    12601 ccggtggtgc gcctgaatgt ggaatccaaa gcaaatacag aattgatgga agaaaaaacg
    12661 caagcaatcc tgacattatt acggaaatag tttttttcgt gtgacataca tatagagtta
    12721 ttccatagtc aattcattta tgcaatcaat gcgatacgaa tccgtaaaag ctatccatat
    12781 tttccaattc tgagttaaca tctaagccac atttcaagcc gcgctctcgt cgcgcggtga
    12841 ccacacctga caggagtatg taatgtccaa gcaacagatc ggcgtcgtcg gtatggcagt
    12901 g
//
