LOCUS       GU445926               11383 bp    DNA     linear   BCT 20-JUN-2010
DEFINITION  Salmonella enterica serogroup O28 O-antigen gene cluster, complete
            sequence.
ACCESSION   GU445926
VERSION     GU445926.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Hu,B., Perepelov,A.V., Liu,B., Shevelev,S.D., Guo,D.,
            Senchenkova,S.N., Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic evidence for the close relationship between
            Escherichia coli O71 and Salmonella enterica O28 O-antigens
  JOURNAL   FEMS Immunol. Med. Microbiol. 59 (2), 161-169 (2010)
   PUBMED   20482625
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Hu,B., Perepelov,A.V., Liu,B., Shevelev,S.D., Guo,D.,
            Senchenkova,S.N., Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:28901"
                     /note="serogroup: O28"
     gene            106..1191
                     /gene="rmlB"
     CDS             106..1191
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADI77012.1"
                     /translation="MKILITGGAGFIGSAVIRHIIKNTQDTVVNIDKLTYAGNLESLS
                     DISESNRYNFEHADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEVARKYWSALGEDKKNSFRFHHISTDEVYGDLPHPDEVENSVTLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KPLPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNVDVVFTICDL
                     LDEIVPKAASYREQITYVADRPGHDRRYAIDAGKISRELGWKPLETFESGIRKTVEWY
                     LANTQWVNNVKSGAYQSWIEQNYEGRQ"
     misc_feature    106..11223
                     /note="O-antigen gene cluster"
     gene            1191..2090
                     /gene="rmlD"
     CDS             1191..2090
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADI77013.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPVGNLIALDVHSKEFCGDFSNPK
                     GVAETVRKLRPDVIVNAAAHTAVDKAESEPELAQLLNATSVEAIAKAANETGAWVVHY
                     STDYVFPGTGDIPWQETDITSPLNVYGKTKLAGEKALQDNCPKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKERQTLSVINDQYGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAG
                     GTTTWHDYAALVFDEARKAGITLALTELNAVPTSAYPTPANRPGNSRLNTEKFQRNFD
                     LILPQWELGVKRMLTEMFTTTTI"
     gene            2132..3010
                     /gene="rmlA"
     CDS             2132..3010
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADI77014.1"
                     /translation="MEMKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLS
                     TLMLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPNPDGLAQAFIIGEEFI
                     GNDDCALVLGDNIFYGHDLPKLMDVAVNKENGATVFAYHVNDPERYGVAEFDQNGTAV
                     SLEEKPLQPKSNYAVTGLYFYDNSVVAMAKNLKPSARGELEITDINRIYMEQGRLSVA
                     MMGRGYAWLDTGTHQSLIEASNFIATIEERQGLKVACPEEIAYRKGFIDIKELKVLAK
                     PLLKNNYGQYLLKIMK"
     gene            3039..3854
                     /gene="wdaK"
     CDS             3039..3854
                     /gene="wdaK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaK"
                     /protein_id="ADI77015.1"
                     /translation="MLFSVLMSLYIREKPEYLHQCLQSLYSQTLPADEIVLVYDGPIS
                     EELDRVVNYWIDILPINIIKLPENVGLGQALNYGLAHCKYDIVARMDTDDICHPDRFE
                     KQIDFLLNNNNVAILGSYIEEFETSPDNVIGKRVVPISHNSIIKRIRKQNPFSHMSVI
                     FKKNKIIQVGGYQHHYLMEDYNLWLRLLTSDVKAANLPSVLVYVRAGKDMINRRSGWR
                     YISSEYKLAKMKHSLGYQNIFISSIYFILRSGPRLFPIFLLKMTYQIIRKKST"
     gene            3885..4436
                     /gene="rmlC"
     CDS             3885..4436
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADI77016.1"
                     /translation="MKKHKTKIHEALIIEPIIYSDARGFFFESYNQLIFEDLIERKIN
                     FCQDNQSLSHKGVLRGLHFQVNPKAQGKLVRCLHGKIYDVIVDLRVDSPTYKKWEGFV
                     LSASNKKQLWIPEGCAHGFLSLEDNTELAYKATEYYSLQHERCIRWNDPSLKIEWPLS
                     DYIISEKDKNGISFSEWENISND"
     gene            4429..4830
                     /gene="qdtA"
     CDS             4429..4830
                     /gene="qdtA"
                     /note="dTDP-4-oxo-6-deoxy-D-glucose-3,4-oxoisomerase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtA"
                     /protein_id="ADI77017.1"
                     /translation="MIDIKFIPLQTHGDDRGSLVALEEGHNIPFKIKRVYYIFDTKKG
                     VHRGFHAHKNLKQVVIAVRGNCKFRLDNGTDSIDVLLDDPTKGLLIESFIWREMYDFS
                     EDCVLMVLADSYYDEQDYIRNYDVFLNLTNN"
     gene            4860..5963
                     /gene="qdtB"
     CDS             4860..5963
                     /gene="qdtB"
                     /note="transaminase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtB"
                     /protein_id="ADI77018.1"
                     /translation="MVDFLSLKKINSRFETDLKEACSRVIDSGWYIMGNELETFEKDF
                     SKYCGVNDTVGVANGLDALILVLRAWIEMGKISPGDEVLVPANTYIASIIAITKNKLV
                     PVFIEPDISTFNIDSSKIEAAITKKTKVILPVHLYGRICPMDEINALAKKYKLLVLED
                     CAQAHGASINNIRVGSWGDAGAFSFYPGKNLGALGDAGAITSNDQEFLSVIKALRNYG
                     SHKKYENQYVGVNSRLDEIQAAMLRVKLKYLDEDNKKRQHIANYYFNSINNPLVSLPA
                     RCRNEENVWHLFVIRTKHRENLQKHLLNHNIQTLIHYPCPPHKQFAYKEFNRLTLPIT
                     EKIHNEVLSLPMDPTLSDEELLEVVNAVNGFNL"
     gene            5960..7225
                     /gene="wzx"
     CDS             5960..7225
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADI77019.1"
                     /translation="MSSFLKVSFFSACLTLFKMLMGFVIAKVIAIYTGPSGMALLGQL
                     QSFVTGVNGIVNAPVGNGIVKYTAEHCDKGSDICSQWWKPAIAFSFSFSIILSIIAIP
                     FSNEISYLLLNSTEYNYLIIITLINLPFTAFGTLIMSITNGQRNYKLYILYGFISTLI
                     ASAIMLVMLIRFGIIGALLSTSLQYGLIGIVLIGLSYRKKWFQRKYFFQRFHKAEVKD
                     ILAYVSMALTSALSLPFTLIIIRSILINYTDISVAGQWQAVWKISEAYIGVVTIALST
                     YFLPKLSSLNNVIDIKCEIRMVLKHILPFVIFFAVVIFFMRDFIIALLFTSEFSFARD
                     LFLVQLLGDVIKIISWVYAFTMLSTKATLWYIGTEVFFCLSWIFASYIFIHTYGAQGV
                     NIAYCLSYFIYFLMIYFNFDRIVKPSSRI"
     gene            7232..8638
                     /gene="wdaN"
     CDS             7232..8638
                     /gene="wdaN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADI77020.1"
                     /translation="MRFIQTLQSVIYNDKDVHDKIRLIKELGSMLWANNIGIYSLNDV
                     ENKIINEVSKKYCPSKIVRSKKNVFVITIGYLSGGHTRLMENLASMLNNECDLIITGN
                     VELEVKNRLNNYFNEIFEIATYEKNDLNEIYKLSDLLSNYFNIILNIHPDDIQSVIAA
                     GLAKKNANSCIHFINHADHVFSFGATVADIWYQISEFGARLDELRELSGKTSFLGIPI
                     AKINKKNAQLSHLKESEVQFIITSGSANKFKPQKTYSLVKKVPDLLIQFPNAKLIIVG
                     TNIIFGYWWWWVYLKFRSRMKFYQTLAYADYLALMDKADIFLDSYPIPGGTAFVEQYL
                     SGKRCAGYISPQQGYTPLERIKCEITCGKIIFNDVKGIDEKIEYIHSYEKVKNRFLTA
                     INSKEITKINWRDYYNWSGDTTFFIDDNIREIPSVILRECIKSRRLAIEMIKNNKLLL
                     LKSLVLKIARISLGKRKK"
     gene            8638..9933
                     /gene="wzy"
     CDS             8638..9933
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADI77021.1"
                     /translation="MEYLIILIFFTLLFFTTKSTWPFGGVNAHPIYFVFLTQIFILTL
                     PGVLILTFFRNCVGSITCEIVTNATKINVMLDYFTILSSIVLAILFSYLLMRGRKDFT
                     PSALSQKRFLNFCFFITILIIIIKIIFVKHVPLFMVLFGNKEAAEIQKAAILRGEDGF
                     SFPVLNFLIKYFPLYFYYSVLITFFKRKISFFYLIIAFATTCLAMLYDLQKAPVVIMI
                     IGSFWLYWAFFGKSKMIIIGALLSLGLVSLLFYISYDFGGNTNYFIDAILNRTFVAQD
                     DGMYWVYQYYDSLPNKELYAYWGIPFAQQFGLPQIDPLSDIIGVVFPNASDNWVNTTT
                     FLLGEAKAIFGSYSLILATFVVFINVIIIMIVSSVLVRISKDLFYPAIFVMLQTIPFA
                     NNITDLIYGRFIFGFLTFMLFPTLFCLLCNGKMSKYDAK"
     gene            9923..10750
                     /gene="wdaM"
     CDS             9923..10750
                     /gene="wdaM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADI77022.1"
                     /translation="MRSDVLIIIVTFNPDWDVVLKRIVSLSKSAVVFVSDNSLDNSVY
                     MMNYHNVIYHFNKGNIGIAKAQNVGIEYALNNKFDFVVFVDDDSNLTAEKINQLLANY
                     EVLGKRGNNIAAFCAYPSERGGLDQKRNSILGDSLLLTNNLMSSGSLTRTEVFKHIGL
                     FDEKLFIDFVDYEWGWRALAKKYLIVIDSSVQFEHSLGQGRLGNGLGVPSPVRHFYQT
                     RNLLWILRLQYVPTLWKLKQLFLFPIRFVYFGFIHEQSKERRAYFLKGLAAGFKNKF"
     gene            complement(10753..11223)
                     /gene="qdtC"
     CDS             complement(10753..11223)
                     /gene="qdtC"
                     /note="transacetylase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtC"
                     /protein_id="ADI77023.1"
                     /translation="MKIHPSSDVKSKKIGSDTTIWQYCVIFEDAVIGNHCNICAHTLI
                     ENKVIIGDNVTIKSGVYIWDGINIEDNVFIGPNVTFTNDIYPRSKKYLERYPTTRVKK
                     NASIGANATILPGITIGQNSIVGAGSVVTRDVPDNVIVVGNPAKFLRPIDDSIA"
BASE COUNT     3540 a   1810 c   2271 g   3762 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgtgtaacga cttgagtaat taatttttat
       61 tggcaaatta aataccacat taaatactcc ttatggaata gaaaagtgaa gatacttatc
      121 actggcgggg caggttttat tggatcagct gttattcgcc atattattaa gaatacacag
      181 gacactgtag ttaatattga taaattaacc tacgccggta atcttgaatc cctttctgat
      241 atttctgaaa gtaatcgcta caattttgaa catgcggata tttgtgattc cgctgaaata
      301 acgcgtattt ttgagcagta ccagccggat gcggtgatgc atttggctgc ggaaagtcat
      361 gtggaccgtt cgattaccgg gccggcagca tttattgaaa ccaatatcgt cggtacctat
      421 gtacttcttg aagttgcgcg taaatactgg tctgcgcttg gcgaagataa aaaaaatagt
      481 tttcgttttc atcatatttc cactgatgaa gtttacggag atttaccgca tcctgatgaa
      541 gttgaaaaca gcgttacgct gccgttattt actgaaacga cggcatatgc gccaagtagc
      601 ccctattctg cgtcaaaagc atccagcgat catttagtcc gtgcctggcg gcgtacctat
      661 ggtctaccaa cgatcgttac caattgttct aataactatg gcccttatca cttccctgaa
      721 aaactgattc cgttggtcat tttgaacgca ctggaaggaa agcctttgcc aatttatggc
      781 aaaggggatc agattcgcga ctggctatat gtagaggatc acgctcgcgc gctttatatt
      841 gtagtgactg aaggtaaggc aggggagact tataacattg gtggacacaa tgagaagaaa
      901 aatgtcgatg tggtatttac catctgtgat cttctggatg agattgtacc caaagcggct
      961 tcttatcgtg aacaaatcac ttatgtcgcg gatcgtccag gccatgatcg tcgttatgcc
     1021 attgatgcag gtaaaattag tcgcgaatta ggctggaaac cgctggagac ttttgaaagc
     1081 ggcattcgta aaacagtgga atggtacctt gcaaatactc aatgggtaaa caatgttaaa
     1141 agtggggcgt atcagagttg gattgaacag aactatgaag gacgccagta atgaatatct
     1201 tactttttgg taagacaggg caagtaggct gggagttgca acgttctcta gcgccagtgg
     1261 ggaatctgat cgccctggat gtccattcaa aagagttttg cggtgatttt agcaatccaa
     1321 aaggcgttgc cgaaactgtt cgtaagcttc gtcccgatgt gattgttaac gcagcagcac
     1381 atactgcagt agataaagca gagtctgaac cagaactagc gcagttactt aacgccacca
     1441 gtgtggaggc tatcgctaaa gcggctaacg aaactggcgc atgggtagtg cattattcaa
     1501 ccgattatgt atttcctggt accggcgata tcccatggca ggaaacagac atcacgtcgc
     1561 cgctaaatgt ctatggcaag accaaactag cgggagaaaa ggccctgcag gataactgcc
     1621 ctaaacacct tatcttccgc accagttggg tttatgcagg taagggcaat aatttcgcaa
     1681 agactatgct tcgtctggcg aaagagcgtc agacactttc ggtcatcaac gatcagtacg
     1741 gtgcgccaac cggcgcggaa ttactggctg actgcacggc tcatgcgatc cgtgtggcgt
     1801 taaagaaacc agaagtagca ggtctttacc atctggtggc cgggggaacc acaacctggc
     1861 atgactacgc ggccttagtc tttgacgagg cgcgcaaggc agggataacg cttgcgctga
     1921 ctgagcttaa tgctgtgccg accagcgcct acccaacgcc ggcgaacaga cccggaaatt
     1981 cgcgtctcaa tactgaaaag tttcagcgta attttgacct tattttgcct caatgggaat
     2041 taggggttaa gcgtatgcta actgaaatgt ttacgacgac aactatctga taaatttaaa
     2101 tgcctgtgag ggcattttct acgaacgaga aatggaaatg aaaacgcgta agggcattat
     2161 tttagcgggt ggctccggta cccgtcttta tcctgtaacc atggcggtaa gtaaacaatt
     2221 acttccaatc tatgataaac cgatgattta ctatccgctt tctacactta tgcttgcagg
     2281 cattcgggat attctgatca tcagtacacc acaggatacg ccgcgttttc aacaactgct
     2341 gggagacggc agccagtggg ggctaaatct tcagtataaa gtacagccga acccggatgg
     2401 tctggcgcag gcatttatta ttggcgaaga atttattggt aacgatgact gtgcattagt
     2461 gctgggggat aatatcttct atggtcacga tctacctaaa ttgatggacg ttgccgttaa
     2521 taaagaaaat ggcgctacag tatttgctta tcatgtaaac gatccggagc gttacggtgt
     2581 ggctgagttt gaccaaaatg gtacagccgt tagtctggaa gaaaaaccgt tacaaccgaa
     2641 gagtaattac gcggtaacag gtctgtattt ttacgataac agcgtggtgg cgatggcgaa
     2701 aaatcttaaa ccttctgctc gcggtgagct agagatcacg gatattaacc gtatctatat
     2761 ggagcaggga agactgtctg ttgccatgat ggggcgcggt tatgcctggc tggatacggg
     2821 tacgcatcag agtttgatcg aagcgagtaa ttttatcgcg acaattgaag aacgtcaggg
     2881 cctgaaagta gcttgcccgg aagagattgc atatcgtaaa ggattcatag atattaaaga
     2941 attaaaagtt cttgctaagc cccttttaaa aaataattat ggccaatatc ttctaaaaat
     3001 tatgaagtaa tagtcggtta cagtatctga gaaatatcat gctcttcagt gttttaatgt
     3061 ctctttatat tagagagaaa ccagaatatt tacatcaatg cttacaaagt ctgtacagcc
     3121 aaacacttcc cgctgacgag atagtgttgg tttatgatgg gccaataagt gaagaattgg
     3181 atagagttgt aaattattgg atagatattt tgccaataaa cattataaaa ctgccagaaa
     3241 atgtggggct tggccaagct ttaaattatg gcctggccca ctgtaaatat gatattgtag
     3301 cccggatgga caccgatgat atatgccatc cggatagatt tgagaaacag attgattttt
     3361 tattgaataa caataatgtg gcaattctgg gctcatatat tgaagaattc gaaacctctc
     3421 cagataacgt cataggtaag agggtggtgc ctataagcca taatagtatt attaagcgca
     3481 tcaggaaaca aaacccgttc agtcatatga gcgtgatatt taagaaaaat aagataatcc
     3541 aagttggagg ctatcaacac cattacttaa tggaggatta taatttatgg ctacgtctgc
     3601 taaccagtga tgttaaagct gcaaatttac catctgtatt agtttatgtc agagctggga
     3661 aagacatgat caatagaagg tcaggatggc gttacatttc cagtgaatat aaattagcta
     3721 agatgaaaca ctcattgggt tatcaaaata tttttatatc ttcaatatat tttatactga
     3781 ggagcggccc ccggcttttt cctatatttc ttttaaaaat gacttatcaa ataatcagga
     3841 aaaaatctac ataaattaat atatatttat tataaagggt aattatgaag aagcataaaa
     3901 caaaaatcca tgaagcgcta attattgaac ctataattta ttctgatgct agaggttttt
     3961 tctttgaaag ctataatcag ctaatttttg aagatttaat tgaaaggaaa attaattttt
     4021 gtcaagataa tcaatcatta tcgcataaag gcgtcttgcg tggattgcat tttcaggtca
     4081 atcctaaagc acagggtaag cttgtaaggt gcttacatgg gaaaatatat gacgtgatcg
     4141 tagatcttcg tgttgactca cctacataca aaaaatggga aggctttgtt ttatctgctt
     4201 caaataaaaa acagttatgg atacctgaag gatgtgcgca tggattcttg tctttagaag
     4261 acaatactga attagcttat aaagctaccg agtattattc cctacaacat gaacgttgta
     4321 tcagatggaa cgatccgtcg cttaaaattg aatggccttt aagtgactat attatttccg
     4381 aaaaagataa gaacggcata agtttttcag agtgggaaaa tatttcaaat gattgatatc
     4441 aaatttattc cactacaaac tcatggtgat gatagaggat cgctagttgc tcttgaagag
     4501 ggacacaata tcccttttaa aataaaacga gtatattaca tttttgatac aaagaaaggt
     4561 gttcatcgtg gttttcacgc gcataaaaat ctaaaacagg ttgtaatagc agtacgaggt
     4621 aattgtaaat tcaggcttga taatggcact gatagtatcg atgttttgct tgatgatcct
     4681 actaaaggac ttttgattga gtcatttatc tggagagaaa tgtatgattt ttctgaagac
     4741 tgtgtattaa tggtcttagc cgattcgtat tatgatgagc aagattacat ccgtaattat
     4801 gatgtttttt taaatttaac aaataattaa ttataaatag aaaaggagag ggtgaactaa
     4861 tggttgattt tttgagttta aagaagatta attctcgctt tgaaactgat ttgaaggagg
     4921 cttgttctag agtcatcgat tctgggtggt atataatggg taacgaatta gaaacttttg
     4981 agaaagattt ttcaaaatat tgtggtgtga atgatactgt tggtgtagca aatggacttg
     5041 atgctttaat tctagtttta agagcttgga tcgaaatggg taaaatttcg cctggtgatg
     5101 aggttttagt ccctgcaaat acatatatag catcaataat tgctattact aaaaataaat
     5161 tggttccagt attcattgag cctgatatat caacgtttaa tattgattca tcaaaaattg
     5221 aagcggctat tactaaaaag acgaaggtga ttttaccagt ccatctatat ggacgtattt
     5281 gtccgatgga tgaaattaat gcgctggcta aaaaatataa actccttgtt cttgaggatt
     5341 gtgctcaggc tcacggagca tctattaata acatccgagt tggtagctgg ggagatgcgg
     5401 gggcatttag cttttatcca ggtaaaaatt taggtgcact tggagatgca ggagcaatta
     5461 caagcaatga ccaagaattt ttaagcgtta taaaagcatt gagaaattat ggttcacata
     5521 aaaaatatga gaaccaatac gttggtgtga acagtcgatt ggatgagatc caggcagcga
     5581 tgcttagagt taaacttaaa taccttgatg aggataataa gaaaaggcaa catattgcta
     5641 attactattt taattcaata aataatccct tagtatcttt accagcaaga tgtagaaatg
     5701 aagaaaatgt atggcattta tttgttataa gaactaaaca tagagaaaat cttcagaaac
     5761 atttacttaa ccataatatc caaactttga ttcattatcc atgtcctcct cataaacaat
     5821 ttgcttataa agaatttaat agattaactt tgcctataac agaaaaaatt cataatgaag
     5881 tgctttcttt accaatggat ccaacactga gtgatgagga attgcttgag gttgtaaatg
     5941 cggtgaatgg ttttaattta tgagttcatt cttaaaagta tcctttttca gtgcatgctt
     6001 gacattattt aaaatgttaa tgggattcgt cattgctaaa gttattgcta tttacacggg
     6061 gccatctgga atggccctac tcgggcagct tcaaagcttt gtgactggtg ttaatggaat
     6121 agtaaatgcc cctgtaggga atggtattgt aaaatatacg gctgagcatt gcgataaagg
     6181 ttcggacatt tgttcgcaat ggtggaagcc agctatagca ttttcgtttt cttttagtat
     6241 aattctatca attatcgcaa ttccattttc taatgagata tcttaccttc tactcaacag
     6301 tactgagtat aactatttaa ttattattac actaattaac ctcccattta cagcatttgg
     6361 gacgttgata atgtccatta ctaatgggca aaggaattat aaactttata ttttatatgg
     6421 ttttatttca acattaatag catcggcgat aatgctagta atgttaatta gatttggaat
     6481 tattggtgca ttactatcta cctcactcca atatggactc attggtatag tccttatagg
     6541 gctaagctac cgaaagaaat ggtttcagag aaaatatttc tttcagcgat tccataaagc
     6601 tgaagtgaaa gatattttag cttatgtatc aatggcactt actagcgctt taagcttacc
     6661 atttacatta attattattc gttccatttt aatcaattac actgatatta gcgttgctgg
     6721 tcaatggcaa gctgtatgga aaatatcaga agcatatatc ggagttgtga cgattgcatt
     6781 aagtacatat tttttaccaa aactgtcatc gctaaataat gtcatagaca tcaagtgtga
     6841 aataagaatg gtattaaagc atattcttcc ttttgttatt ttttttgcgg tagtgatatt
     6901 ttttatgcga gattttatta tcgcattgtt atttacttcc gaattctctt ttgctcgaga
     6961 tttatttttg gtgcagttgc taggcgatgt tattaaaatt attagttggg tttatgcctt
     7021 tacaatgctt tctactaaag caacactatg gtatattggt acggaagtat ttttttgtct
     7081 gtcatggatt tttgcgagct atatttttat ccatacatat ggagcgcagg gggttaatat
     7141 agcatattgc cttagttatt ttatttactt ccttatgatt tactttaatt tcgacagaat
     7201 tgtcaaacca tcaagtagga tataatattt catgaggttt attcaaacgc ttcaatctgt
     7261 aatatataat gataaagatg ttcatgataa aattagatta atcaaagaat taggtagtat
     7321 gttgtgggct aataatatag gtatttacag tctgaatgac gttgaaaata aaataataaa
     7381 tgaagtcagc aaaaaatatt gcccaagtaa aatagttaga agtaagaaaa acgtatttgt
     7441 tattactatt ggctatctat cgggggggca tactcgtttg atggaaaatt tagcgagtat
     7501 gctaaataat gagtgcgatc ttatcataac aggaaatgtt gaactagagg ttaaaaatag
     7561 gcttaataac tattttaatg aaatatttga gatagccaca tatgagaaaa atgatctaaa
     7621 tgagatttat aagttaagtg acctcctctc aaattatttt aatattattt taaatattca
     7681 tccagatgat attcaaagtg tcattgcagc tggcttggca aaaaaaaatg caaatagttg
     7741 tattcatttt atcaatcatg ctgatcatgt ttttagtttc ggtgctactg ttgccgatat
     7801 ttggtatcaa attagtgagt ttggtgcaag gctagacgaa ctccgagagt tgtcaggtaa
     7861 gacctccttt cttggaattc ctatcgcaaa gataaataaa aaaaatgcac aactttcgca
     7921 tttgaaggaa agtgaagtac agtttattat cacttctggt tctgctaata aattcaaacc
     7981 acaaaaaacg tactctttgg taaagaaggt gccagattta ttaatacagt ttcctaatgc
     8041 taaattaatc atagttggta caaatattat atttggctat tggtggtggt gggtttattt
     8101 aaaattcaga tctcgtatga aattttatca aacattggct tatgcagatt atcttgctct
     8161 tatggataaa gcagacatct ttcttgatag ttaccctatt cctggtggta ctgcttttgt
     8221 agaacaatat ctttcaggta aacgttgtgc tggatatatt tctccacagc aaggctatac
     8281 acctctagaa agaataaaat gtgaaattac ctgcgggaaa ataattttta atgatgtcaa
     8341 aggcattgat gaaaaaattg aatatataca ttcttatgaa aaagttaaaa atagatttct
     8401 aacagcaatt aattctaaag aaatcaccaa gattaattgg agagattatt ataattggag
     8461 tggtgacact acttttttta ttgatgataa tatacgagaa attcccagtg ttattcttag
     8521 agaatgtatt aaaagccgaa gattagctat tgagatgatt aaaaataata aacttttact
     8581 tttaaaatcg ttagtattaa aaattgcgcg aatatcatta ggtaagcgga aaaaataatg
     8641 gaatatttaa ttattttgat tttctttact ctcttatttt ttacgactaa aagtacatgg
     8701 ccttttggtg gagtgaatgc tcatcctata tattttgtat ttcttactca aatttttata
     8761 ttaacgctcc ccggtgttct tattttaaca tttttcagga actgtgttgg atctattaca
     8821 tgtgaaattg tcactaatgc cactaaaata aatgtgatgc ttgattattt tacaattctt
     8881 agttctatcg ttttggcaat attattttcc tacttattaa tgcgagggcg taaagatttt
     8941 actccttctg cgttgtctca aaaacgtttt cttaattttt gtttttttat aacaattcta
     9001 attattataa ttaaaattat ctttgtgaag catgttcctc tctttatggt tttatttggc
     9061 aacaaagagg ctgcagaaat tcaaaaggca gctattttac gtggtgagga tgggttttct
     9121 tttcctgttc tcaattttct tattaagtat tttcctctct atttttacta ctcagtatta
     9181 ataacttttt ttaagcgcaa aattagcttt ttttatttaa taatagcttt tgctacaaca
     9241 tgtttagcaa tgctttatga tttacaaaaa gcaccggtag tcattatgat tataggttca
     9301 ttttggctat actgggcttt ttttggtaaa tctaaaatga ttatcattgg ggcattacta
     9361 agcttaggat tggttagttt attattttat atctcgtatg attttggtgg aaatacaaat
     9421 tattttattg acgctattct caataggact tttgttgcgc aagatgatgg tatgtactgg
     9481 gtatatcaat attatgattc attgcctaat aaagaactat acgcctattg gggtatacct
     9541 tttgctcaac agtttggttt accacagata gatcctttaa gtgatattat cggtgtggta
     9601 tttccaaatg ctagtgataa ttgggttaat acaacaacat ttcttctggg ggaggctaaa
     9661 gcaatattcg gaagttatag tttgattctt gctacttttg tagtattcat taatgtgatt
     9721 attataatga tagtttcatc agtattggtt agaatatcaa aagacctttt ttatccagct
     9781 atatttgtta tgttgcaaac aattccattt gctaataata tcacggattt gatttatggc
     9841 cgatttatat ttggttttct gacgtttatg ctatttccga cacttttttg tttgctatgt
     9901 aacggcaaga tgagtaaata cgatgcgaag tgatgtatta ataattattg taactttcaa
     9961 tcccgactgg gatgtcgttt taaagagaat tgtttcctta agtaaatcag cagtagtttt
    10021 cgtttctgac aatagtctcg ataattctgt atatatgatg aattatcata atgtaatcta
    10081 tcacttcaat aagggtaata ttggtatagc aaaagcgcaa aatgttggta ttgaatatgc
    10141 cttaaataac aagtttgatt ttgttgtctt tgttgacgac gacagtaatc ttaccgctga
    10201 aaaaataaat cagctactgg ctaactatga agtacttgga aaacgtggta ataacatagc
    10261 tgcattttgc gcatatccca gtgaaagagg cggtttagac cagaagagaa attcaatact
    10321 tggagatagt ttattgctca cgaataacct aatgagctct ggttcgctta ctaggactga
    10381 ggttttcaag catattggtt tatttgatga aaaattattt attgactttg ttgattatga
    10441 atgggggtgg agggcattag caaaaaaata tctcatagtt attgattcat cagtacaatt
    10501 tgaacattct cttggacaag ggcgtttagg aaatggctta ggtgtacctt ctccagtaag
    10561 acatttttac cagacgcgaa atcttctgtg gattttacgc ttacagtatg ttcctacatt
    10621 atggaaatta aaacaattat tcctttttcc gattcgattt gtttattttg gttttattca
    10681 tgagcaatca aaagagcggc gagcctattt tcttaaaggt ctcgccgcag gttttaaaaa
    10741 taaattttaa agttaagcaa ttgaatcatc aattggacgt agaaatttag caggattacc
    10801 aacgacgatg acattatcgg gtacatctct ggtaacaaca gaccctgcac caacaatgga
    10861 attttgtcca atggttattc caggtaaaat tgtggcattt gctccgatag aagcattttt
    10921 cttaactcgc gtcgttggat acctttctag gtattttttt gatcgtggat aaatatcatt
    10981 ggtaaaggtt acattcggac cgataaaaac attatcttca atgtttatac catcccaaat
    11041 ataaacgccc gactttatcg taacattgtc tcctatgatg actttatttt caatgagcgt
    11101 atgtgcacat atattacaat ggtttcctat cactgcatct tcaaatatga cacaatattg
    11161 ccaaatcgtt gtgtctgagc caattttttt tgacttaaca tcgcttgatg gatgaatttt
    11221 catattgttt atccaatacc tataaataga ttttttgaat gcttttatat tatatagtgt
    11281 gctacgttat ttttatagta atatcttaaa cagaactaac cagtaatctg acaggagtat
    11341 gtaatgtcca agcaacagat cggcgtcgtc ggtatggcag tgt
//
