LOCUS       JX975339               12856 bp    DNA     linear   BCT 27-AUG-2013
DEFINITION  Salmonella enterica strain G1466 O antigen gene cluster, complete
            sequence.
ACCESSION   JX975339
VERSION     JX975339.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Reeves,P.R. and Wang,L.
  TITLE     Structural diversity in Salmonella O antigens and its genetic basis
  JOURNAL   FEMS Microbiol. Rev. (2013) In press
   PUBMED   23848592
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Reeves,P.R. and
            Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1466"
                     /db_xref="taxon:28901"
                     /note="serogroup: O43"
     misc_feature    1..12856
                     /note="O antigen gene cluster"
     gene            61..1080
                     /gene="gne"
     CDS             61..1080
                     /gene="gne"
                     /note="Gne"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-GlcNAc-4-epimerase"
                     /protein_id="AFW04793.1"
                     /translation="MMILVTGGAGYIGSHTCLALIAKGHDIIVIDNLSNSSYESIRRV
                     ENLTNKKIKFIKSDIKERHCLDEVFNNYSIEAVIHFAALKSVSESVEKPLVYYDNNIT
                     GTLTLLAAMQSANVNNLIFSSSATVYGSSSIVPNKENDTIGGTTNPYGTSKYFSEIIL
                     QDFCKSNELFSAVSLRYFNPTGAHKSGMIGEDPNGIPNNLIPYISQVATGKLKYLNVY
                     GGDYDTIDGTGMRDYIHVDDLAEGHVKTLEHMLSTKVGYDVFNLGSGRAYSVLQIIKA
                     FERVTKKEIPYNIVARRNGDLDKSWADTEKARNLLGWEAKYGIEDMLQDVWNWQSHNP
                     NGYRV"
     gene            1117..2241
                     /gene="wfbG"
     CDS             1117..2241
                     /gene="wfbG"
                     /note="WfbG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04794.1"
                     /translation="MKILMIGTTVQSLLGFRYELLKDLVSAGHEVYALSVDYDHKSKQ
                     TLIDIGVVPIDYTISRSGINPFKDFVNFIFLYKLIKKITPDIVFSYFTKPCIWGSLAA
                     YYAGVHKIIAMLEGLGFLFTEQPSGIPQTTKILKRIQILLYRIAFKKINTLIVLNEDD
                     KNDLLNAGCIEDRKVYVLGGIGINLEHYKYSPAPLEPISFIFIGRLLREKGIYEFIAA
                     AKNIKERFEGVTFNVLGNIDPANPGSLKKNELDTIIKEEIINYVGYVNDVSHWLSKSS
                     VFVLPSYREGFPRSTQEAMAIGRAVITTDVPGCRDTVVNNSNGFLIQRWSSDALAEAM
                     EKFILSKELIRDCGKESARIAKEKFDTRITNKKIITILLS"
     gene            2289..3407
                     /gene="gmd"
     CDS             2289..3407
                     /gene="gmd"
                     /note="Gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AFW04795.1"
                     /translation="MNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHSCNPKFHLHYGDLTDASNLTRILQEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWMMLQQEQPEDFVIATGVQYSVRQFVELAAAQLGIKLRF
                     EGEGINEKGIVVSVTGHDAPGVKPGDVIVAVDPRYFRPAEVETLLGDPSKAHEKLGWK
                     PEITLSEMVSEMVANDLEAAKKHSLLKSHGFDVNLSLE"
     gene            3410..4375
                     /gene="fcl"
     CDS             3410..4375
                     /gene="fcl"
                     /note="Fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-fucose synthetase"
                     /protein_id="AFW04796.1"
                     /translation="MTKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDG
                     RAVQAFFAGAGIDQVYLAAAKVGGIVANNTYPADFIYENMMIESNIIHAAHLHNVNKL
                     LFLGSSCIYPKLARQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMA
                     AASIYVMELAREVWQENTAPMLSHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLAGTYQWFLENQQRFRG"
     gene            4378..4878
                     /gene="gmm"
     CDS             4378..4878
                     /gene="gmm"
                     /note="Gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosylhydrolase"
                     /protein_id="AFW04797.1"
                     /translation="MFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGY
                     WFVPGGRVCKDETLEAAFARLTQAELGVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHY
                     IVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLAGENVHENSRAYFQNEPHSVIGLDK
                     KDVKYV"
     gene            4871..6319
                     /gene="manC"
     CDS             4871..6319
                     /gene="manC"
                     /note="ManC"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphorylase"
                     /protein_id="AFW04798.1"
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSRELYPKQFLQLSGKNSLLQTT
                     LLRLSALACNEPLVITNEQHRFIVAEQLRQIDQLHNNIILEPYGRNTAPAIALAALSA
                     LRRKEQEDPLLLVLAADHIIAKEEVFCNAVKKAVPIAENGNIVTFGIIPEYAETGYGY
                     IERGVPFSVNANSTETDFYHVKKFVEKPDRLKAEKYISTGDYFWNSGIFMFKATVYLN
                     ELKKFRPDIYSVCEQVLSSSYRDLDFIRLPETLFKDCPAESIDFAVMEKTERCIVCPI
                     DIGWSDVGSWQSLWDISEKTDEGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKSDVQDVKKIVEMLKAQERTEYIAHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLDDKTMLVTANESIYIPLGATYSLENP
                     GVIPLNLIEVSSGDYLGEDDIVRQKERYRKDD"
     gene            6323..7699
                     /gene="manB"
     CDS             6323..7699
                     /gene="manB"
                     /note="ManB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AFW04799.1"
                     /translation="MNKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDARGDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNEAARGSYRQISLRDAYIDHLL
                     GYISVNNLTPLKLVVNSGNGAAGPVIDAIEARLKALGAPVEFTKIHNIPDGTFPNGIP
                     NPLLPECRDDTRKAVIEHGVDMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KHPGAKIIHDPRLTWNTEAVVTAAGGTPVMSKTGHAFIKERMRTEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGQSLGELVRDRMAAFPASGEINSRLAEPAAAIAR
                     VEAHFAEEAQAVDRTDGLSMSFAGWRFNLRSSNTEPVVRLNVESRGDRLLLNTKKELI
                     FEILNNKN"
     gene            7731..8948
                     /gene="wzx"
     CDS             7731..8948
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFW04800.1"
                     /translation="MIKLLLNSSLLKTIVILFNLLIPAVVIRSYSAYDFGIYSYLLVY
                     AIVVSILQNGVTASFRNIISNLENKEILSCLLFTIKRVTFPTLTILFISGVFVSLLLG
                     VDTIYGKIAIFVSVSLINIFYPLVASYFDARGKTVKFVLYEIIFSVLSLALIYILGLF
                     KVNILFVCIITANYRGVYAVFLLVYKYIGTSSRYSDMYNKNKKYVIFCFEDILFVSIQ
                     LINVINSLLFMNMLAGYYGVISFGIFALYYRFVSFPQQIVGFSASLIWIRFRQIYFDN
                     KNMSREFLKNLLFVFALLLISWFFIVHFLMETIIRIYSSKILIYPGDLILLNIMICLV
                     LVKDFTSIILNAINVYKAQIILNFFLLFINLLFWKFNYLPQFDTVYLVILSVLTLICI
                     LVNSFFIKNKVFR"
     gene            8948..9652
                     /gene="wcmB"
     CDS             8948..9652
                     /gene="wcmB"
                     /note="WcmB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04801.1"
                     /translation="MTINILYICTGEYRNFFDKFYSSCEGYFIPEYKKKYYVFTDSHS
                     DKFSKYSNVTVVPVENNCWPLNTLLRFSYFSKIVSDLQPNTYTFFFNANALIVKTIPF
                     DIFKNANLVGVVHPGYKNKMSIFYPWERKKSAVCYLSYFKKGIYFQGCFNGGRTEYFC
                     DLIKTCNDMTIKDLKRNIIAKVHDESYLNYYFYFKEPLCLSELYSWPEKYGENTEAKI
                     IMRDKEREDWYANIKS"
     gene            9633..10976
                     /gene="wzy"
     CDS             9633..10976
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFW04802.1"
                     /translation="MQILKAKESNKQSVLFICMLVFFYLLTFVLFLLDEGLFSFLFFI
                     EALLVLLCCLFVVFSEKKNGFLNPLSFFIISTFFFIIVRPIFYSLSIAENVNEVITAG
                     YSIDKNYLFYSLFVVNICSALTILSCLLLRPLCKSITVNIYDLYFYNGNVSRLFMLSG
                     VIFTGVFLIKSYSKFILLGDVSVFEVDKYGLHDDLYWFTFAKYSYIVSLLFAKRKDFI
                     FYSLLIFLASIGYIMVGLRGYTISYAFLLLFFLDLRYKLKFRYLLVFAILVAIVSSLI
                     LNYRIGIEVNRGFWEMIFNPLLQQGASFETVYGVLKYHSSVLNCISYYDYFFSIKDIG
                     SCIDSARGIYFAEGGSFATSFYSELIYFGWFIGGIALLLFSFSLSFIQSCYDCIVKNK
                     RISENYTYKLILFLLLPNLIYFARSSLFDFSIKLVFVLFFIILLWFMKKTLVNVK"
     gene            10998..11735
                     /gene="wcmC"
     CDS             10998..11735
                     /gene="wcmC"
                     /note="WcmC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04803.1"
                     /translation="MISVIMAVNRLDKYVHISIESILKQTYQDFELIIVANGIDCARI
                     VRELSTFSESDYRVKIYSSPIGQLAFALNFAISKCNYSIVARMDSDDISFPERLEKQY
                     KYLQCNKLDLVGTAICLIDENDSFIKKITYPGGPAIKKNLPFKNCFAHPSVMFRKDFF
                     LKYRGYCGGFNSEDYDLWFRMLSDSPRWDNMLEPLLSYRVHANSTQKSKLAYYECASY
                     SLREFLKNRTVINFFSLIYHCSKALIK"
     gene            11756..12661
                     /gene="wcmD"
     CDS             11756..12661
                     /gene="wcmD"
                     /note="WcmD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04804.1"
                     /translation="MFSCLSGGLGNQMFQYSAAYILKKNICHAQLIIDDSYFYCQPQK
                     DTPRNFEINQFNIVFDRVTTDEEKRAISKLRKFKKIPLPLFKSNVITEFLFGKSLLTD
                     EDFYKVLKKNQFTVKMNACLFSLYQDSSLINKYRDLILPLFTINDELLQVCQQLDSYG
                     FICEHTNTTSLHIRRGDYVTNPHAAKFHGTLSMNYYSQAMNYVDHKLGKQLFIIFSDD
                     VQWAAEKFGGRSDCYIVNNVNCQFSAIDMYLMSLCNNNIIANSTYSWWGAWLNKSEEK
                     LVIAPRKWFAEDKESLLAVNDWISI"
BASE COUNT     3668 a   2294 c   2873 g   4021 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgtgtttcaa tttttaattg aaaggtgcgg
       61 atgatgatat tagtcactgg tggagctggt tatataggat cgcatacttg tttagcgctt
      121 attgccaaag ggcacgatat tattgttatt gataatttaa gtaattcctc ttatgagtcg
      181 atcaggcgcg ttgagaattt aaccaataaa aaaataaaat ttataaaaag tgatataaaa
      241 gaacgacatt gcctggatga agtgtttaat aattattcga tagaagcagt aatacatttt
      301 gcagcattaa aatcagtaag tgagtctgtt gaaaaaccat tggtttatta tgataataat
      361 atcacaggta ccttgactct actggctgca atgcaaagtg caaatgtaaa taatttgatt
      421 tttagttcgt ctgccaccgt atatggttct tcttcgattg ttccaaataa agagaacgat
      481 acaattgggg ggactacaaa tccctatgga acatcaaaat attttagtga gattattctg
      541 caggattttt gtaaatctaa tgagttattt tccgcagtaa gtcttcgtta ttttaatcct
      601 actggtgctc ataaatcagg tatgattggg gaagatccta atggaatacc aaataacctc
      661 atcccatata tatcacaagt tgccactgga aaacttaaat atttaaatgt atatggtggt
      721 gattatgaca caattgatgg cactggcatg cgagattata ttcatgtaga tgatttagct
      781 gaaggacatg tcaagacatt agaacacatg ttatcaacaa aagtaggtta tgatgttttt
      841 aatttggggt caggtcgagc atatagcgtt ttgcaaatca ttaaagcttt tgagcgcgtc
      901 actaaaaaag agattcccta taatattgtc gcgcgaagaa atggtgattt agataaatct
      961 tgggctgata ctgaaaaagc cagaaacctc ctggggtggg aggcaaaata tggaatagag
     1021 gatatgttgc aagatgtttg gaactggcaa tcacacaatc caaatggtta tagagtttaa
     1081 taatcagtat cgtaaccgga attttataaa aagaacatga aaatattaat gattggtact
     1141 acggtgcaat ctttattagg attcagatat gaattactta aagatttagt tagcgcagga
     1201 catgaagtat atgcgttatc tgttgattat gatcacaaat cgaagcagac attgattgat
     1261 atcggtgtcg taccgataga ttatacaata agtcgctcag gaattaatcc cttcaaggac
     1321 tttgttaatt ttattttttt atataagcta ataaaaaaaa taacaccaga catcgttttt
     1381 tcttatttta ctaagccttg tatttgggga tcgcttgccg catattatgc tggtgttcat
     1441 aaaattatcg cgatgcttga agggttaggt tttttattta ccgaacaacc atctgggatt
     1501 ccccaaacga caaaaatcct aaaaagaata cagattctgc tctatagaat agcttttaag
     1561 aaaattaata cgcttattgt acttaatgaa gatgataaga atgatttact taacgctggt
     1621 tgtatagaag atagaaaagt atatgtattg gggggtattg gaataaattt agagcattac
     1681 aagtattcac cagcaccact tgaaccaatt tcttttattt tcattggccg attgctcaga
     1741 gaaaagggta tttatgaatt tatcgctgct gctaaaaata taaaggaaag atttgaaggg
     1801 gtgacgttta atgtgctggg taatattgat ccagcaaacc ctggtagttt aaaaaaaaat
     1861 gagcttgata caattattaa agaagaaata attaactatg taggatatgt gaatgatgtt
     1921 tctcattggt taagtaagag cagtgttttc gttttacctt cttatagaga agggtttcct
     1981 cgaagtacac aggaggctat ggctatagga agagctgtaa taactaccga tgttcctgga
     2041 tgtagagata ctgttgttaa taattctaat ggttttttaa tacaacggtg gtctagcgat
     2101 gctcttgcag aggcgatgga aaaatttatt ttatctaaag aattgattcg cgactgtggt
     2161 aaagaaagtg ccaggatagc taaagaaaaa tttgacaccc gaattaccaa taagaaaata
     2221 attactatat tattgtctta acttaatata cctactttaa attaaattta tatgtattag
     2281 gaattactat gaataaagtc gctctcatta ctggcgtaac cggacaggat gggtcttacc
     2341 tggcagaatt tctgctggaa aaagggtatg aggtgcatgg tatcaagcgc cgcgcgtcat
     2401 cgtttaatac cgagcgcgtg gaccatattt atcaggaccc gcacagctgc aacccgaaat
     2461 ttcatctgca ttatggcgac ctgaccgacg cctccaacct gacccgcatt ttacaggaag
     2521 tgcagccgga tgaggtctat aacctgggcg caatgagcca cgtggcggtg tcgtttgagt
     2581 cgccggaata taccgccgat gtggatgcga tgggcacgct gcgcctgctg gaggcgatcc
     2641 gcttcctcgg tcttgaaaag aaaacgcggt tctaccaggc ctccacctct gaactgtacg
     2701 gtctggtgca ggagatcccg cagaaagaga ccacgccgtt ttacccgcgt tccccctatg
     2761 cggtggcgaa actgtacgcc tactggatca ccgtcaacta ccgtgaatcc tacggtattt
     2821 acgcctgtaa cggcattctg tttaaccatg agtccccgcg tcgcggcgaa accttcgtca
     2881 cccgtaagat cacccgcgcc atcgccaata tcgcccaggg gctggagtcc tgcctgtatc
     2941 tcggcaacat ggactcgctg cgcgactggg gtcatgcgaa agattacgtg cggatgcagt
     3001 ggatgatgtt acagcaggag cagccggaag atttcgtgat tgccaccggc gtgcagtatt
     3061 ccgtgcgcca gtttgtggag ctggcggcgg cgcaactggg gataaaactg cgctttgaag
     3121 gcgaaggcat taatgagaaa gggatcgtgg tatccgttac cggacacgat gcgccgggcg
     3181 tgaaaccggg ggatgtgatt gtggccgttg atccgcgtta tttccgtccg gcggaagtgg
     3241 aaaccctgct gggcgacccg tccaaagcgc atgagaaact gggctggaaa ccggaaatca
     3301 ccctgtcgga gatggtctcc gagatggtgg cgaacgatct ggaggccgcg aaaaaacact
     3361 cactgctgaa atctcacgga ttcgatgtaa atttatcttt ggaatgacca tgaccaagca
     3421 acgaattttt gtggcgggcc atcgcgggat ggtgggctcc gccattgtac ggcagcttgc
     3481 gcagcgcggc gacgtggaac tggtgctgcg cacccgcgat gagctggatc tgctcgacgg
     3541 gcgcgcggta caggcgttct ttgccggggc gggtatcgac caggtttatc tggcggcggc
     3601 gaaagtgggc ggcattgtcg ccaacaacac gtatccggcg gattttattt atgaaaacat
     3661 gatgatagag agcaacatta ttcacgccgc gcacctgcac aacgtgaaca aactgctgtt
     3721 tctcggttcg tcctgtatct atccgaaact ggcaaggcag ccgatggcgg aaagcgagct
     3781 gctgcagggg acgctggagc cgaccaatga gccgtacgcc atcgccaaga tcgccgggat
     3841 taaactgtgc gagtcctaca accggcagta cggtcgcgac taccgttcgg tgatgccaac
     3901 caacctgtac ggcccgcatg acaatttcca tccggacaat tcacatgtga tcccggcgct
     3961 gctgcgccgc tttcatgagg ccgcgcagag ccacgcgccg gaggtggtgg tgtggggcag
     4021 cggcacgccg atgcgcgaat ttctgcacgt tgacgatatg gcggcggcca gcatttacgt
     4081 gatggagctg gcgcgcgaag tgtggcagga gaacaccgcc ccgatgctgt cgcacattaa
     4141 cgtcggcact ggcgtggact gcactatccg cgagctggcg cagaccatcg caaaggtggt
     4201 gggttaccag ggccgggtgg tgttcgacgc cgcgaagccg gacggcacgc cgcgtaaatt
     4261 gctcgacgtc acgcgcctgc atcagcttgg ctggtatcac gaaatttcac tggaggcagg
     4321 gcttgccggt acttaccagt ggttccttga gaatcagcaa cggttccggg ggtgacaatg
     4381 tttttacgtc aggaagattt cgccgccgtg gtgcggacca cgcccctcat ctccctcgat
     4441 ttcattgtgg aaaacggcca gggggaaatt ttactgggcc agcgtctcaa ccgtccggcg
     4501 cagggctact ggtttgtgcc gggggggcgg gtgtgcaaag acgaaacgct ggaggccgcc
     4561 tttgcacgcc tgacgcaggc ggaactgggc gtgcgtctgc cgctggcggc agggacgttt
     4621 tatggcgtct ggcagcactt ctatgacgac aacttttccg gtgaggattt ttcaactcac
     4681 tacatcgtgc tcggctttcg tctgcgcgtg gcggagagcg atttacgcct gcctgatgcc
     4741 cagcatggca gttaccgctg gctgacgccg gaacagcttc tggcaggcga gaacgtccac
     4801 gagaatagtc gagcttattt tcaaaatgaa ccccattctg tcattggttt agacaaaaag
     4861 gacgtcaagt atgtctgatg tacccttaat tgctgttgta atggctggtg gtacagggag
     4921 ccgtctttgg cctctatctc gagaacttta cccaaaacaa tttttacaac tttctggaaa
     4981 aaattcctta ttacagacaa ccctgcttcg gttgtcggca cttgcatgta atgaaccatt
     5041 ggttataaca aacgagcagc accgttttat tgttgcggag cagttacggc aaatagatca
     5101 actacataat aatatcattc ttgaaccgta tgggagaaat acagctccag cgattgctct
     5161 tgctgcatta tcagcgctta gacgtaagga gcaggaggat cctttacttt tagttcttgc
     5221 agccgatcat attatcgcta aagaagaagt tttctgtaat gccgttaaga aagcagtccc
     5281 cattgccgaa aacggcaata ttgttacatt tggtatcata cctgaatatg ctgagactgg
     5341 ttatggctat atagagcgtg gtgtgccttt ctctgtaaat gcaaattcaa ctgaaacaga
     5401 tttttatcat gtgaaaaaat ttgttgaaaa gccggataga ttaaaagcag agaaatatat
     5461 ttcaacaggg gattatttct ggaatagcgg catatttatg tttaaagcca ctgtttatct
     5521 gaatgagtta aaaaaattca gaccagatat ttatagcgtt tgtgagcagg tgctttcttc
     5581 ttcatatcgc gatcttgatt ttattcgact tccagaaact ttatttaaag attgtcctgc
     5641 agaatctatt gattttgctg ttatggaaaa aacggagcgt tgcattgtct gcccgattga
     5701 tattggttgg agtgatgttg gttcctggca gtctttatgg gatatcagcg aaaaaacaga
     5761 tgagggagat gtttgtaagg gcgacattct gacatacaac actaaaaata actacatata
     5821 ctcagagtca gcattggttg ctgctgtagg tgttgaagat attgttattg tacaaacaaa
     5881 agatgccata cttgtttcta aaaaatctga tgtccaggat gtaaaaaaga tcgttgagat
     5941 gctaaaggct caagagcgta cagagtatat tgcccatcgt gaggtttttc gtccatgggg
     6001 taaattcgac tcaatagatc agggtgagcg atataaagtt aagaagatca tcgtcaaacc
     6061 aggagaaggg ctttccttgc gtatgcatca tcatcgttca gaacactgga ttgtactttc
     6121 tggtacggct aaagtcaccc tggatgataa aacgatgcta gtgactgcca atgagtcgat
     6181 ttatattcct ttgggggcta cttatagtct ggagaatcca ggagttattc cacttaatct
     6241 catcgaagtt agttcaggag attatttagg tgaagatgat atagtccggc agaaagaacg
     6301 ttacagaaaa gatgattgaa tcatgaataa attaacctgt ttcaaggcct acgacattcg
     6361 cggcaggctg ggcgaagagc tcaatgaaga tatcgcgtgg cggattggcc gcgcctacgg
     6421 cgaatattta aaaccgaaaa ccatcgtgct gggcggcgac gtgcgcctga ccagcgaggc
     6481 gctgaagctg gcgctggcga aagggttaca ggatgcccgc ggcgacgtgc tggatatcgg
     6541 tatgtccggc accgaagaga tttacttcgc caccttccac ctcggcgtgg acggcggtat
     6601 tgaagtgacc gccagccata acccgatgga ctacaacggc atgaagctgg tgcgcgaggg
     6661 cgcccgcccg ataagcggcg acaccggtct gcgcgacgtt cagcgtctgg cggaagcagg
     6721 cgacttcccg ccggtgaacg aggccgcgcg cggcagctac agacaaatca gcctgcgcga
     6781 cgcctacatc gaccacctgc tcggctacat cagtgttaac aatctcaccc cgctgaagct
     6841 ggtggtgaat tccggcaacg gcgcggcggg gccggtcatt gacgccatcg aagcgcgcct
     6901 gaaggcgctg ggcgcgccgg tggagttcac caaaatccac aacatcccgg acggcacctt
     6961 cccgaacggc attcccaacc cgttgctgcc ggagtgtcgt gacgacaccc gcaaggccgt
     7021 tattgaacac ggggtggaca tgggcatcgc ctttgacggc gacttcgacc gctgcttcct
     7081 gtttgatgag aaagggcagt ttatcgaggg gtactacatc gtcgggctgc tggcggaagc
     7141 gtttctggaa aaacacccgg gggcgaagat tatccacgac ccgcgcctga cctggaacac
     7201 ggaggcagtg gtgacggcgg cgggcgggac gccggtgatg tcaaaaaccg ggcacgcctt
     7261 tatcaaggag cggatgcgta cggaggatgc catttatggc ggggaaatga gcgcgcatca
     7321 ctattttcgt gattttgcct actgcgacag cgggatgatc ccgtggctgc tggtggcgga
     7381 gctggtatgc ctgaaggggc agtcgctggg tgaactggtt cgcgaccgga tggcggcgtt
     7441 cccggcgagc ggggaaatca acagcagact ggcggagccg gcggcggcca tcgcgcgggt
     7501 ggaagcgcac ttcgcggaag aggcgcaggc agtggaccgc acggacggtc tcagcatgtc
     7561 gttcgccggc tggcgtttca acctgcgctc gtcaaacacc gagccggtgg tgcgcctgaa
     7621 tgtggaatca cgcggtgata gattgttact aaatacaaaa aaagagctta tctttgagat
     7681 acttaacaat aaaaactaaa ttatattatt actaaggtaa taagatttaa atgataaagt
     7741 tacttttaaa ttcgtcttta ttaaagacga tagttatttt atttaactta ctgatacctg
     7801 cagtagttat tagatcttac tcagcatatg atttcggtat ttactcatat ttattagtat
     7861 atgctattgt tgtttctatt ctccaaaatg gagttacggc aagctttcga aatataatat
     7921 ccaatcttga gaataaagag atccttagct gtttattgtt tacgataaaa agagtgacct
     7981 ttccaacact tactatactg tttatatccg gagtttttgt ttctcttttg ctgggggtgg
     8041 atactatata tggcaagata gctatatttg tttctgtttc tttaataaat atattttatc
     8101 cactggtggc atcatatttt gatgctagag gaaagacagt aaaatttgtt ttatatgaaa
     8161 ttatattttc tgtattatcg cttgccttaa tttatatttt agggcttttt aaagtaaata
     8221 ttctctttgt ttgtataatt acagctaatt acagaggggt atatgcggta tttttactag
     8281 tatataaata tatcggcacc agcagccgtt actcagatat gtataataaa aacaaaaaat
     8341 atgttatttt ttgttttgag gatatattat ttgtaagtat acaattaatt aacgtaatta
     8401 attcattgct tttcatgaat atgcttgctg gctattatgg tgtaatttca tttggcattt
     8461 ttgcattgta ttatagattt gtatctttcc ctcaacaaat cgtgggtttt tctgcatctc
     8521 ttatttggat aagatttagg cagatatatt tcgataataa gaatatgtcg agagagtttt
     8581 taaaaaattt attatttgta tttgctcttc tattaatctc ttggttcttt attgttcatt
     8641 tcttaatgga gacaataata cgaatatact cctctaaaat tcttatctac ccaggggatt
     8701 tgattcttct aaatataatg atttgccttg ttttagtgaa ggattttact agcataatat
     8761 taaatgccat taatgtatat aaagcacaaa taattttaaa tttttttcta ctctttataa
     8821 atttattgtt ttggaaattt aactacttac ctcaattcga tacagtgtac ttggttattt
     8881 tatcagtgtt gactctaatc tgcatacttg taaattcgtt ttttataaaa aataaggtgt
     8941 ttaggtaatg acaataaata ttttatatat atgcacaggt gaatatagaa atttttttga
     9001 taaattttac tcttcttgcg aagggtactt tattcctgaa tataaaaaaa aatactatgt
     9061 atttacagat tctcatagtg ataaatttag taaatacagt aatgtaactg tagttccggt
     9121 tgaaaataat tgctggcctc ttaatacatt gctacggttt tcatattttt ctaagatagt
     9181 ttctgactta caaccaaata catacacctt tttttttaat gcaaatgcat taatagttaa
     9241 aactatccca tttgatatat ttaagaatgc taatttagtc ggggttgttc atcctggtta
     9301 taaaaataaa atgtctatct tttatccttg ggaacgaaaa aagtcggctg tatgctattt
     9361 aagttacttt aaaaaaggta tatatttcca aggatgcttc aatggaggta ggacggaata
     9421 tttttgtgat ttgattaaga cttgcaatga tatgacaatt aaagatctta aaagaaatat
     9481 tatagcaaaa gtacatgatg aatcttattt aaattattat ttttacttta aagaaccttt
     9541 atgcctctca gaattgtatt cttggcccga aaaatatggt gaaaatacgg aggctaaaat
     9601 tatcatgcgt gataaggaaa gagaagactg gtatgcaaat attaaaagct aaagaaagta
     9661 ataaacaatc tgttctattt atttgcatgc ttgttttttt ttacttactt acttttgttt
     9721 tgtttctttt agatgaaggt ttatttagtt ttcttttttt tattgaagct ttgctggtat
     9781 tattatgttg cttgtttgtt gtgttttcag aaaagaaaaa tggtttttta aatcctttat
     9841 ctttttttat tatatcaaca ttttttttta taatagttag acctattttc tattcactga
     9901 gtatagctga aaatgtgaat gaggttatca ctgctggtta ttctatagat aagaattatt
     9961 tattttattc tttatttgta gtgaatattt gttcagcttt gacgatactt agctgtttat
    10021 tgctaaggcc attatgtaaa tctattactg tcaatatata tgacttatat ttttataatg
    10081 gaaatgtatc tcgtcttttt atgttatcag gagtaatttt tacaggtgtt tttttaataa
    10141 aatcatattc taaatttatc ctgttaggtg atgtttcggt ttttgaggta gataaatatg
    10201 gtttacatga cgacctttac tggttcacat ttgcgaaata ttcatatatt gtgtctttgt
    10261 tgtttgctaa acgtaaagat tttatttttt actcccttct aatctttctt gcatctatag
    10321 gctatattat ggtaggtcta agagggtata ctatatctta tgcttttcta ttgttattct
    10381 ttttagatct tagatataaa ttaaagttta ggtatttact agtttttgct attctggtag
    10441 ctatagtttc atctcttatt cttaattaca ggataggtat cgaggtaaat cgtggctttt
    10501 gggaaatgat atttaatccc ttattacagc aaggggcctc ttttgaaacc gtatatggag
    10561 ttttaaaata tcatagtagc gtacttaatt gtatttctta ttatgactat tttttttcaa
    10621 ttaaggatat tggcagttgt attgatagcg cgcgtggtat atattttgct gaaggtggaa
    10681 gttttgcaac aagcttttat agtgagttga tatactttgg ttggtttatt ggaggcatag
    10741 ccttgcttct gttttctttc tcattatcct ttattcagtc ctgttatgat tgtattgtaa
    10801 aaaacaaaag aatatcagaa aattacacat ataaattaat tttattccta ttattaccaa
    10861 accttatata ctttgctcgt tcttctctat ttgattttag tattaagctg gtttttgtat
    10921 tgttttttat tattcttttg tggtttatga aaaaaacatt agtgaatgtt aaataaagtg
    10981 atttaggaag tgctataatg atatcggtga taatggcagt taatagactt gataaatatg
    11041 ttcatatatc aatagaaagt attcttaaac aaacctatca agattttgaa ttaataattg
    11101 ttgctaacgg aattgattgt gcaagaatcg taagagagtt aagtacgttt tcagaatcag
    11161 actatagggt gaagatatat agttcaccta tcggacagtt agcatttgca ttgaatttcg
    11221 ctatatcaaa gtgtaattat tcaattgtgg caagaatgga ttctgacgac atttcatttc
    11281 cagaacgcct tgaaaaacag tataaatatc tgcaatgtaa taagcttgac ttagttggaa
    11341 cagcaatttg cttaattgat gaaaatgatt cttttattaa aaaaatcacg tatcctggcg
    11401 gacctgcaat aaaaaaaaat ttaccattta aaaactgctt tgctcatcca tcggtaatgt
    11461 ttagaaagga tttttttcta aaatatagag gctattgtgg cgggtttaat tctgaagatt
    11521 atgatctgtg gttcagaatg ctgagtgatt cgccgcgctg ggacaatatg cttgaacccc
    11581 tgctaagcta tcgtgtacat gccaattcta cgcagaagtc aaaacttgcc tattatgagt
    11641 gtgctagcta ttcattgcgc gagtttttaa aaaacaggac agttattaac tttttctcat
    11701 tgatttatca ttgttctaag gctctaataa aataaattac taaaaggcgg tgactatgtt
    11761 tagttgttta tctggtgggc tagggaatca aatgttccaa tattctgctg catatatttt
    11821 aaagaaaaat atatgtcatg ctcaattgat aatagatgat agttattttt attgtcagcc
    11881 acaaaaagat actccaagaa attttgaaat taatcaattt aatattgttt ttgatcgggt
    11941 tactactgat gaagaaaaaa gagcaatatc taaactaaga aaatttaaaa aaatacctct
    12001 tcctttattt aaatctaacg tcataaccga gtttttattt ggcaaaagtt tattgacaga
    12061 tgaggatttt tataaagtct taaagaaaaa tcaatttaca gttaaaatga acgcttgttt
    12121 attttcactt tatcaagatt cttccctcat aaataaatat cgggatttga tattaccatt
    12181 gtttactatt aatgatgaat tgttacaggt atgtcagcag ttagatagtt atggttttat
    12241 ttgtgaacat accaatacaa cgtctctgca tatccgacgt ggtgattatg taacaaatcc
    12301 tcatgctgct aaatttcatg gtacattatc tatgaactat tatagtcaag ctatgaatta
    12361 tgtagatcat aaattaggaa aacaattatt cattatattt tctgatgatg tgcaatgggc
    12421 tgcagaaaaa tttggaggtc gttctgattg ttatatagtt aataatgtga attgtcagtt
    12481 tagtgctata gatatgtatt tgatgtcttt gtgtaataat aatattattg caaatagtac
    12541 atatagttgg tggggggctt ggctaaataa gtcagaagaa aaattagtta ttgccccccg
    12601 gaaatggttt gcagaagata aagaatcatt gttggctgta aatgattgga ttagcattta
    12661 aaaacttatg atggtataca ttgctctaat tctatctaag gcaatgtatt gctaactttc
    12721 tatagtctat tcgttaggtt aagtttcagt aaacatctaa gccacatttc aagccgcgct
    12781 ctcgtcgcgc ggtaaccaca cctgacagga gtatgtaatg tccaagcaac agatcggcgt
    12841 cgtcggtatg gcagtg
//
