LOCUS       JX975332               15261 bp    DNA     linear   BCT 27-AUG-2013
DEFINITION  Salmonella enterica strain G1605 O antigen gene cluster, complete
            sequence.
ACCESSION   JX975332
VERSION     JX975332.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 15261)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Reeves,P.R. and Wang,L.
  TITLE     Structural diversity in Salmonella O antigens and its genetic basis
  JOURNAL   FEMS Microbiol. Rev. (2013) In press
   PUBMED   23848592
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15261)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Reeves,P.R. and
            Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15261
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1605"
                     /db_xref="taxon:28901"
                     /note="serogroup: O45"
     misc_feature    1..15261
                     /note="O antigen gene cluster"
     gene            1145..2449
                     /gene="wzx"
     CDS             1145..2449
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFW04713.1"
                     /translation="MLGRYIKKININVEIKGIISNTGWLLFDKFIRLIFGLLVGVWVA
                     RYLGPDTYGQLAYVITLISFFQVVSLLGMDTILVRDIAHKKDQANEILGTAIIMRFGA
                     GLLCLIVAVSFVGFFNGTYFAILTLLAGGSLIFQCIDTIDIWFQSQSQSKRTVIAKLC
                     SYVVTNIIRIFLILCHAPVWGFALLLSIDALFSALALSCAYTSFKTTDKWRITLSRGK
                     NLLRESWPFLISGISIIMYMRLDQIMIKNLLGEKSLGIYAAVLQFSLLWNFVPITLSV
                     SLAPYIARKKKENEQAYYNAINNVFIIFSSIGWGITLTISILSPFIINFMLGSSYADG
                     AVVLSIHVVTNLFICLGIAQSLWIVNERKGLFILYKSLTGLLVCGFSNLILIPLYGIK
                     GAAIAAVLSQLSASMLFNLVFAKKIFLMQIKSLFLIKTITKI"
     gene            2452..3363
                     /gene="wdbB"
     CDS             2452..3363
                     /gene="wdbB"
                     /note="WdbB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04714.1"
                     /translation="MKKYSPVIAFVYSRPKHAKKMLLALRKNSLAKYTDLIIYSDGAK
                     NKKDEPGVQAVRNYISTLTGFRSVTVVMQEKNIGLANSIIKGVTATVKKYGRVIVLED
                     DIETSPYFLEYMNDALNMYVDVKSVASISASSYPVAMNNSDQTTYLLRIPLCWGWATW
                     EDRWSMFKKDINAIDSIDKRIQNYINFDGAFDFFEQATLNAKGKLNTWFIFWYITLAS
                     KELLTLFPTKVMAKNIGHDGSGENCDDNDIYKNTMISNERVMLTLKETLVEDKDYYLA
                     HVKYFKKIKKNHVLKFFSYVKRKIIGA"
     gene            3365..4045
                     /gene="wdbC"
     CDS             3365..4045
                     /gene="wdbC"
                     /note="WdbC"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AFW04715.1"
                     /translation="MSLLNRIKSHLVKSTLNKNGKGIYIDFLSYVDEKSTFEGYNRIT
                     GKSSIYNSHIGSYTYAVGASIGNATVGKFCSIAMGAKVGGLGSHPTSYVSTHPIFYSA
                     KRQCGISFSEEDYFKEDNKTVIGNDVWIGANAIIMDGVHVGDGAIIAAGAIVTKDVPP
                     YAIVTGIPAVIKRFRCSIEQVQILQHLEWWNWPQHLLKKHTKSFQGPIDDNLEDLAKV
                     KLDPKGKN"
     gene            4046..5209
                     /gene="wzy"
     CDS             4046..5209
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFW04716.1"
                     /translation="MSYKSNNFYRNILLLSFFFSGFSLYTVFIPLLFLTILLSLHKNK
                     ALYFGRLAASANILFIILFILFVFFIGEDRLLLEKPFKYAAYIIFMIFCLTLLLLNNI
                     EYAQKAILFFIFGIFLRAEIIVIYSFLDPSGVYGYGKLLDPFNHEEINSPGISNSLAM
                     VFVFLLVVFEPKTLARKIFLILVYPLLLLSSIFLGGRAFFIIAILGLVYQYKNNTNIK
                     AIIFLLFSALGCLVLFFIMFKDAPVFEKYFNLIFERFGSEGLKSARFELIQDGISKFF
                     MYPFGGFTPYASEYSGSWYHNIYLDSARVAGFLPLIMFLISNIILFSCYFKCKKRNHK
                     NKYFFLSVITLIIMCQDVILEGNMMLLICYALFTTMQLQTGGRSKNEIILQSK"
     gene            5184..6326
                     /gene="wdbD"
     CDS             5184..6326
                     /gene="wdbD"
                     /note="WdbD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04717.1"
                     /translation="MKLYYRVNDYYPFNIEGSSAASSVRHRVNRVCRCLPDDIELKIY
                     SIVKSSKVSTLKRECYDISQSHQCVTYFVATNVLGGWLKGGISIWLMIYLTLKTLGKK
                     NFSIIYNILPANILIHMLFSFLYPHLLSKTIFQVEEIYSVYDFNFLKRKLIKISEKSV
                     FNRGLNFIIVNENIRKLIRKKEANTIVDYGYDSRKGKSIKINPSDNIVYTGRLDYVGG
                     VEILLQALADLPVSIKDKVIITGSGGLKDKTISYAHEIGVHYKGFLSDIEYESLLSNA
                     KVSINPLRSKCKFSQYSFPSKVLQYLEYGCVVISSGIAQPELEKFFADELLIYMDDDP
                     LKLNHLIRAVDELEFDKSVIINKYQNFMEMKEKKLNTFFYKIFRGK"
     gene            6439..6987
                     /gene="wdbE"
     CDS             6439..6987
                     /gene="wdbE"
                     /note="WdbE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04718.1"
                     /translation="MDAPYILRAINALSSTLFCKDYYRELQIKQLSGLSYEVINKLAA
                     DFVEELNEKRISSTNNLLDEYRRKGYTIVLASATIEPLARAIAKKIGVDYFSSEVEFV
                     NEQCSGKLNRDLLFFKKEALLHLIKKSENIVIVTDNFTDLELIQVSDYCHIVVRNDKD
                     IRKWNKQLKNHLCNIVFIKKNV"
     gene            6987..7784
                     /gene="wdbF"
     CDS             6987..7784
                     /gene="wdbF"
                     /codon_start=1
                     /transl_table=11
                     /product="WdbF"
                     /protein_id="AFW04719.1"
                     /translation="MNKYIIFFPFGYTYISRIKNLSKFISFLLTIPIPCFLFFYYGLV
                     SSENKSCFTGLVFIWSYINLYIFYENGYIENDIKTVKKEENPTLRIVGDLYHFIDNNY
                     YKIISIRVVFFVISILLLFFVSNYILILKLSLLSLATALLYYFHNNIRSVFKVLTFFC
                     LSSSRFIFPLLVCSDLIAPEKFNYILLSIFIYTIPASLIYSAKSFKLIRFLLRGEYKY
                     KILYYTFTSFIFMASHFLYDKNDISLFMFFLLLYMSLLICLKKVINR"
     gene            7786..8598
                     /gene="wdbG"
     CDS             7786..8598
                     /gene="wdbG"
                     /note="WdbG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04720.1"
                     /translation="MEAVMSKVYCRLAGGLGNQIFQYGASLLISKKKHVPFIQLDDSG
                     LSTYKAIRSNELPNYFKLEKAIFKKSNILKLRLPKMKFITPFANNFIGDLNFNRLIDK
                     NLKSKNYYLDGYFQSCLNQSVFDEMLSALKQDYIYSSINVKKNVCAIHIRGGDFLTEK
                     YSDIASVDYYYEKLTKVKELNIAERFIIVTDDRSYASELAKKISLDYSFSEGSMLEDF
                     LLIAQSQIKILSNSTFSIWASALGFQPGCIVFAPQMLTVKDTRNFILPGELI"
     gene            8621..9736
                     /gene="gmd"
     CDS             8621..9736
                     /gene="gmd"
                     /note="Gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AFW04721.1"
                     /translation="MKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVD
                     HIYQDPHSCNPKFHLHYGDLTDASNLTRILQEVQPDEVYNLGAMSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQEQPEDFVIATGVQYSVRQFVELAAAQLGIKLRFE
                     GEGIDEKGIVVSVTGHDAPGVKAGDVIVAVDPRYFRPAEVETLLGDPSKAHEKLGWKP
                     EIALSEMVSEMVANDLEAAKKHSLLKSHGFNVNLSLE"
     gene            9733..10704
                     /gene="fcl"
     CDS             9733..10704
                     /gene="fcl"
                     /note="Fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-fucose synthetase"
                     /protein_id="AFW04722.1"
                     /translation="MTMNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLL
                     DGRAVQAFFAGAGIDQVYLAAAKVGGIVANNTYPADFIYENMMIESNIIHAAHLHNVN
                     KLLFLGSSCIYPKLARQPMAESELLQGALEPTNEPYAIAKIAGIKLCESYNRQYGRDY
                     RSVMPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDD
                     MAAASIHVMELAREVWQENTAPMLSHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDA
                     AKPDGTPRKLLDVTRLHQLGWYHEISLEAGLAGTYQWFLENQQRFRG"
     gene            10707..11207
                     /gene="gmm"
     CDS             10707..11207
                     /gene="gmm"
                     /note="Gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosylhydrolase"
                     /protein_id="AFW04723.1"
                     /translation="MFLRQEDFAAVVRTTPLISLDFIVENSQGKILLGQRLNRPAQGY
                     WFVPGGRVCKDETLEAAFERLTQAELGVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHY
                     IVLGFRLRVAQGDLHLPDAQHGSYRWLTPEQLLAGDNVHENSRAYFQNEPHSVIGLDK
                     KDVKYV"
     gene            11200..12648
                     /gene="manC"
     CDS             11200..12648
                     /gene="manC"
                     /note="ManC"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphorylase"
                     /protein_id="AFW04724.1"
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSRELYPKQFLQLSGKNSLLQTT
                     LLRLSALACNEPLVITNEQHRFIVAEQLRQIDQLHNNIILEPYGRNTAPAIALAALSA
                     LKRKEQEDPLLLVLAADHIIAKEKVFCNAVKKAVPIAENGNIVTFGIIPEYAETGYGY
                     IERGAPFSVNANSTETDFYHVKNFVEKPDRLKAEKYISTGDYFWNSGIFMFKATVYLN
                     ELKKFRPDIYSICEQVLSSSYRDLDFIRLPETLFKDCPAESIDFAVMEKTERCIVCPV
                     DIGWSDVGSWQSLWDISEKTDEGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKSDVQDVKKIVEMLKAQERTEYIAHREVFRPWGKFDSIDQGKRYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLDDKTMLVTANESIYIPLGATYSLENP
                     GVIPLNLIEVSSGDYLGEDDIVRQKERYRKDD"
     gene            12652..14019
                     /gene="manB"
     CDS             12652..14019
                     /gene="manB"
                     /note="ManB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AFW04725.1"
                     /translation="MNKLTCFKAYDIRGRLGEELNEDIAWRIGRACGEYLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNEAARGSYRQITLRDAYIDHLL
                     GYISVKNLTPLKLVVNSGNGAAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPNGIP
                     NPLLPECRDDTRKAVIEHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KHPGAKIIHDPRLTRNTEAVVTAAGGTPVMSKTGHAFIKERMRTEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGQSLGELVRDRMAAFPASGEINSRLAEPAAAIAR
                     VEAHFAEEAQAVDRTDGLSMSFPDWRFNLRSSNTEPVVRLNVESRGNEHLMQSKTVEI
                     IKILN"
     gene            14100..14912
                     /gene="wdbH"
     CDS             14100..14912
                     /gene="wdbH"
                     /note="WdbH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04726.1"
                     /translation="MNHSVAIILSVYKAEKPAYLYQSLSSLFSQTMSADIYLYIDGEI
                     TVSLENVINDFKPRPNFYIIRGKDNKGLAFALNSLIDISLRNGYKYIARMDTDDISRL
                     DRIEQQFRFMEKRTDVDVLGGYCHEFGSEYALELKKVPIDHNSLKKYSIIYCPFIHPT
                     VMFRSSVFKDGIRYPLDTRYTEDIALWLCLLDKGYCFHNLPKVLLDYRINEDTFSRRR
                     GFKKAVSEFSVRFNYMRSLRNFSIKNWCVLIVRFLFHMLPLPFIRYAYKKYR"
BASE COUNT     4479 a   2731 c   3411 g   4640 t
ORIGIN
        1 ggctgcaggg atcaaagaaa tcctcctggt aactcacgcg tctaaaaacg ccgttgagaa
       61 ccacttcgac acctcttatg aacttgaatc acttcttgag cagcgcgtta agcgccagct
      121 tctggcggaa gtgcaatcta tctgcccgcc gggcgtgact atcatgaacg ttcgccaggc
      181 acagccgtta gggctggggc actctattct gtgcgcgcgt ccggtcgtgg gcgataatcc
      241 tttcattgtg gtactcccgg atattattat tgatgatgct accgccgatc cgctgcgcta
      301 taaccttgcg gcgatggtgg cgcgcttcaa tgaaactggt cgcagccaag tgctggcgaa
      361 gcgcatgaaa ggtgatttat cggagtattc tgttatccag acgaaagaac ctctggataa
      421 tgaaggcaaa gtcagccgga ttgtggagtt tatcgaaaaa ccggatcagc cgcagacgct
      481 ggattccgat ttgatggcgg taggccgtta tgtgctttca gccgatatct gggcggaact
      541 ggaaagaacc gaaccgggcg cctggggccg catccagctc acagatgcca ttgctgaact
      601 ggcgaaaaaa cagtctgttg acgcgatgct aatgacgggc gacagctacg actgcggtaa
      661 aaaaatgggc tacatgcagg catttgtgaa atacgggctg cgtaacctga aagaaggggc
      721 gaagttccgt aaaagcatag agcagctatt gcatgagtaa gtattaacaa ccgtgataaa
      781 tggttggtga tagacataat aacggcagtg aacattcgaa gcggcaagtt ggctgaaacg
      841 agtgttgact gccgttttaa ttttgtataa agggcttaag taacaagggg ttatctggag
      901 cattttaatg ctgattttat aagattaatc cttgtttccg gatgcaatta ataagacaat
      961 tagcgtttaa gttttagtga actttgccct gctgggcgag gtttgtaaca agtcgatatg
     1021 tacgcagtgc actggtagct gatgagccag gggcggtggc gttgcaaaaa ttaaacataa
     1081 taaaacctaa agatttttgc taggttttat ttctattaca ttgaataatt ttgcaggggc
     1141 cttgatgcta ggcaggtata taaaaaaaat aaatataaat gttgagatta aaggtataat
     1201 ttcaaataca gggtggttac tatttgataa attcattcgt ttaatatttg gcttgttagt
     1261 tggcgtatgg gttgctcgct atctggggcc ggatacttac ggtcagttag cttatgtgat
     1321 tactcttatt agctttttcc aagttgtttc actactcggt atggacacaa tattagttcg
     1381 cgatattgcg cataaaaaag atcaagctaa tgaaattttg ggtacagcaa tcattatgcg
     1441 ttttggtgct ggattgctct gtctgatagt agcagttagt tttgtaggtt tttttaatgg
     1501 tacctatttt gcaatactca cattgttagc tggcggtagt ttaatatttc aatgtataga
     1561 cactattgat atctggtttc aaagtcaaag ccagagtaag aggactgtta ttgcaaaatt
     1621 atgctcgtat gtagttacga atataatacg gatttttctt attttatgtc atgctccagt
     1681 ttgggggttc gctttattac tcagtattga tgccttattc tcagcactgg ctttgtcatg
     1741 tgcgtacacc tccttcaaaa ctacagacaa atggaggatt acgttatcaa ggggtaaaaa
     1801 tctacttcgt gagtcatggc catttttgat aagtggtatc tccattatta tgtatatgcg
     1861 actcgaccaa ataatgataa aaaatttatt gggagaaaaa tcgctaggta tttatgctgc
     1921 agtgctacaa ttttcattat tatggaattt cgtccctata actttatcag ttagtcttgc
     1981 tccatatatt gcaagaaaga aaaaagagaa tgagcaggca tattataatg caataaataa
     2041 tgtgtttatt attttttcat ctatcgggtg gggaataaca ttaacaattt cgatattgtc
     2101 acctttcatt ataaatttca tgttgggatc tagttacgct gatggagctg tcgtcctctc
     2161 tattcatgtt gtgacaaatc tgttcatatg tttgggaatt gctcaaagcc tttggattgt
     2221 taatgagagg aaaggtttgt tcatacttta taaatcactt acaggccttt tagtttgtgg
     2281 atttagcaat ttaatcctaa taccgctgta tggaataaaa ggagctgcaa ttgctgcagt
     2341 actatcacag ttgtccgcct ctatgctttt caatttagtt tttgctaaaa aaatattttt
     2401 aatgcaaatt aaaagtttat ttcttattaa aacaattacc aagatttgaa aatgaaaaaa
     2461 tattctccgg taatcgcttt tgtttattca agaccaaagc atgcgaaaaa aatgttattg
     2521 gcattaagaa aaaacagtct agcaaaatat acagacttaa ttatatattc agacggtgca
     2581 aaaaataaaa aggatgagcc aggtgtacag gcagtaagga actatattag cactctaacc
     2641 ggttttagaa gcgtcactgt tgtgatgcag gaaaaaaata tcgggcttgc taatagtatc
     2701 attaaaggtg taacagctac ggtaaaaaaa tacggacgtg taatagttct tgaagatgat
     2761 attgagacat ctccgtattt tcttgaatat atgaatgatg ctttaaacat gtatgtcgat
     2821 gttaagtcgg tggcttcaat ctccgcaagc agttaccctg ttgctatgaa taatagcgat
     2881 cagactacct atttattaag aatccctcta tgctggggat gggcaacttg ggaggatagg
     2941 tggtctatgt ttaaaaaaga tataaatgct atagattcaa tcgacaaacg aattcagaat
     3001 tatataaatt ttgacggtgc atttgatttt tttgaacagg caacattaaa cgcaaagggg
     3061 aaacttaata cttggttcat tttctggtac ataactcttg cctctaaaga attattgact
     3121 ctatttccaa caaaggtaat ggcaaaaaat attggacatg atgggtctgg agaaaattgt
     3181 gatgataatg atatatataa aaatacaatg atttctaacg aaagagtaat gttaacttta
     3241 aaagaaactt tggtagaaga taaagattat tatttggctc atgtaaaata ttttaaaaag
     3301 ataaaaaaaa atcatgtgct aaaattcttt tcctatgtta aaagaaaaat tataggagca
     3361 taatgtgagt cttttaaata gaattaaatc acatcttgta aaaagcactt taaataaaaa
     3421 tggtaaagga atttatattg atttcttatc gtatgtcgat gaaaaatcaa cttttgaagg
     3481 gtacaataga attacgggga agtcttctat ttataattca catattggaa gttataccta
     3541 cgctgtaggg gccagcatcg gaaatgctac ggtgggtaaa ttctgtagta ttgctatggg
     3601 agctaaggtt ggtggactag gatctcatcc aacgtcatat gtttctacac atccgatatt
     3661 ttattctgca aaaaggcagt gcggcatctc attttcagaa gaagactatt ttaaagaaga
     3721 taacaaaaca gttattggta acgatgtgtg gattggtgcc aatgctatta ttatggatgg
     3781 ggtacatgtt ggtgatggag caataattgc tgctggtgct attgtaacta aagatgtacc
     3841 accttatgca atagtaacag gaattcctgc tgtaattaaa agatttcgct gttccattga
     3901 acaggtccaa atattacaac atttagagtg gtggaactgg cctcaacatc ttttgaagaa
     3961 acatacgaaa tcgtttcaag ggcctattga tgataattta gaagatttag ctaaagtaaa
     4021 attagaccca aagggaaaaa attaaatgtc gtacaaaagt aataatttct atagaaatat
     4081 acttttattg agttttttct tttctggctt tagcttgtat actgttttta taccgttact
     4141 atttttgact attcttcttt ctttgcacaa aaataaggca ctgtattttg gaaggctagc
     4201 agcaagcgct aatatattat ttattatttt atttatttta tttgtttttt ttatagggga
     4261 agataggcta ttattagaaa aaccatttaa atatgcagca tatattatat ttatgatttt
     4321 ttgtttgaca ttattactat tgaataatat agaatatgca cagaaagcaa tactattttt
     4381 tatttttgga atatttttac gagcagagat tattgttata tatagtttcc ttgacccatc
     4441 aggagtctat ggatatggta aattattaga tccttttaac catgaagaaa ttaactcacc
     4501 aggtatcagt aattcactgg ctatggtttt tgtattttta ttagttgttt tcgaaccgaa
     4561 gacattagcc agaaaaattt ttcttatact agtttacccg ttacttttat tatcctctat
     4621 atttttagga gggcgagcat tttttataat tgcaattctt gggttggttt atcaatacaa
     4681 aaataataca aatataaagg cgattatttt tttattattt tctgctcttg gatgcttagt
     4741 gctatttttt attatgttta aggacgctcc agttttcgaa aaatatttta atttaatttt
     4801 tgagcgtttt ggaagtgaag gattaaaatc agcacgcttt gaactaatac aggacgggat
     4861 tagtaaattc tttatgtatc cgtttggagg gtttactcca tatgcaagtg aatactcagg
     4921 tagttggtat cataatatat acttggatag tgccagagtt gcaggttttt tacctttgat
     4981 aatgtttctt atatcaaata ttattttatt ttcatgctat ttcaagtgta aaaagcgtaa
     5041 tcacaaaaat aaatattttt tcttgtcagt tattacctta attataatgt gccaggacgt
     5101 tattctcgaa ggaaatatga tgttgctgat ttgttatgca ttattcacaa caatgcaact
     5161 tcaaacggga gggagaagta aaaatgaaat tatattacag agtaaatgat tattatccgt
     5221 ttaatatcga aggtagctct gctgctagtt cagtacgaca tagggttaat agagtttgcc
     5281 gatgccttcc tgatgatatc gaactaaaaa tatactctat tgtaaaatca tccaaggtat
     5341 cgaccttgaa acgtgaatgc tacgatattt cgcaatcaca tcaatgcgtt acttactttg
     5401 ttgctacaaa cgttttagga ggatggttaa agggggggat ttctatttgg ttaatgatct
     5461 atttaacact aaaaacgcta ggtaaaaaaa attttagtat catatataat atattgccgg
     5521 caaatatact aattcatatg cttttttcat ttctttatcc ccatttactg tcgaaaacaa
     5581 tatttcaggt agaagagata tatagtgttt acgattttaa tttcctaaaa agaaaattaa
     5641 ttaaaatttc agaaaagtct gtatttaatc gcggtttaaa tttcatcatt gtaaatgaaa
     5701 atatcagaaa acttatcaga aaaaaagagg cgaatacaat tgtagattac ggttacgatt
     5761 caagaaaagg aaaaagtatt aaaataaatc cctcggataa tatcgtttat acaggaagat
     5821 tggattatgt tggtggcgtt gaaattcttt tacaagcttt ggctgatcta cctgtgagta
     5881 ttaaagataa agtaataatt actggcagtg gcggtttaaa agacaaaact atcagttatg
     5941 cccatgaaat tggggtccac tataaaggat ttcttagtga tatcgaatat gagtctttgc
     6001 tatccaatgc aaaagtaagc attaatcctt tacgctcaaa atgtaaattt tctcagtatt
     6061 ctttcccctc aaaagtactt cagtatcttg aatatgggtg tgttgtaatt tcgagtggga
     6121 ttgcacaacc agagctggaa aaattttttg ctgatgagtt attaatctat atggatgatg
     6181 atccgctcaa acttaatcat cttatcaggg cagtcgatga gcttgaattt gataagtcgg
     6241 taatcataaa taagtatcag aactttatgg aaatgaagga aaaaaaatta aatacgttct
     6301 tttataagat atttagaggg aaataacttg aaactggcta tttttgatat ttgtggtact
     6361 ttatattttg ataatacaac ttactctttt ttatgttggt tgtcgaataa aggtattata
     6421 aagtataata agaaaataat ggatgctcct tatattttaa gagctattaa tgcattatct
     6481 tctactttat tttgtaaaga ctattataga gaattgcaga ttaagcaact atcgggatta
     6541 tcttatgagg ttattaataa gttagctgca gactttgtag aagagctcaa tgaaaagcgt
     6601 atttctagta caaataattt actcgacgaa tatagaagaa aaggatatac aatagtactt
     6661 gcaagcgcaa ctattgagcc tctcgcaaga gctattgcga aaaaaattgg tgttgattat
     6721 ttttcgtcgg aagtggagtt tgtaaatgag caatgttctg gtaaactgaa tagagatcta
     6781 ctctttttta aaaaagaagc attattacat ctcattaaaa aatccgagaa tatcgttatc
     6841 gttacagata attttactga tttagagtta attcaggtca gtgattactg tcatattgtt
     6901 gtacgcaatg ataaagatat tcgaaaatgg aataaacaac ttaaaaatca tctttgcaac
     6961 attgttttca ttaagaagaa tgtttaatga ataaatacat catttttttt ccattcggat
     7021 atacttatat atcacgaatt aaaaatctct ctaagtttat tagttttttg ctaacaatac
     7081 ctataccatg ctttctcttc ttttactatg gcttggtatc atctgaaaat aagagttgct
     7141 ttactggctt ggtatttatc tggagctata tcaatttata tatattttat gaaaatggat
     7201 atatagaaaa tgatattaaa acggtaaaaa aagaagaaaa cccaacgttg cgtattgttg
     7261 gtgatttata tcattttata gataataact attacaagat aatcagtatc agggtggtgt
     7321 tctttgtaat atctattttg ctattatttt ttgtaagtaa ctatatactg attttaaagc
     7381 tttcattact ttctttggcg acggctctac tttattattt tcataataat attagaagtg
     7441 tgtttaaagt tttaactttt ttttgtttat catcctcaag atttatattt cctttattgg
     7501 tatgttcaga tttaatagca cctgaaaaat ttaattatat actattatct atatttatat
     7561 atacaattcc agcatcactt atttattcgg cgaaatcatt taagctaatt aggtttttac
     7621 tcaggggaga gtacaaatat aaaatattat attatacctt tacttctttt atttttatgg
     7681 cgtcgcactt tttgtacgac aaaaatgata tttcattatt tatgtttttt ttgcttttat
     7741 atatgtcatt gcttatttgt ttaaaaaaag taattaatag gtgaaatgga ggcagtgatg
     7801 tctaaagtat attgtaggtt agctggagga ttaggtaatc aaatattcca gtacggtgct
     7861 tcacttctta ttagcaaaaa aaaacacgtg ccttttatac aactggatga ttctggattg
     7921 agcacttata aagcaattag gagtaatgaa ctacctaatt attttaaatt agaaaaagca
     7981 atatttaaaa aaagtaatat attaaaactg cgcctgccga aaatgaaatt tattacaccg
     8041 ttcgctaata attttatcgg cgatttaaat ttcaatagat tgattgataa aaatttaaag
     8101 tctaagaatt attatcttga tggttatttt caaagctgcc ttaatcagtc agtatttgac
     8161 gagatgctta gtgctttaaa gcaagattat atttattcaa gtattaatgt taagaagaat
     8221 gtatgtgcta tacatatacg aggaggagat tttttgactg agaagtatag tgatattgct
     8281 tccgttgatt actattatga aaaattgacc aaggttaaag aattaaatat agcagagaga
     8341 tttattattg tgacggatga taggagctat gcttcggagc tagcaaaaaa aatatctctc
     8401 gattactctt tttcagaggg gagtatgtta gaggattttt tgttaatagc ccagtctcag
     8461 attaaaatat tatccaatag cacattctca atttgggcat cggcattagg ttttcaacca
     8521 ggttgtatag tatttgcccc tcaaatgtta accgttaaag atactcggaa ttttatttta
     8581 ccaggtgaat tgatttaaaa agataaggag ttatttaata atgaaagtcg ctctcattac
     8641 tggcgtaacc ggacaggatg ggtcttacct ggcagaattt ctgctggaaa aagggtatga
     8701 ggtgcatggt atcaagcgcc gcgcgtcatc atttaatacc gagcgcgtgg accatattta
     8761 tcaggacccg cacagctgca acccgaaatt tcatctgcat tatggtgacc tgaccgacgc
     8821 ctccaacctg acccgtattt tacaggaagt gcagccggat gaggtttaca acctgggcgc
     8881 gatgagccac gtggcggtgt cgtttgagtc gccggaatat accgccgatg tggatgcgat
     8941 gggcacgctg cgcctgctgg aggcgatccg cttcctcggt cttgaaaaga aaacgcggtt
     9001 ctaccaggct tccacctctg aactgtatgg tctggtacag gagatcccgc agaaagagac
     9061 cacgccgttc tacccgcgtt ccccctatgc ggtggcgaaa ctgtacgcct actggatcac
     9121 cgtcaactac cgtgaatcct acggtattta cgcctgtaac ggcattctgt ttaaccacga
     9181 gtccccgcgt cgcggcgaaa ccttcgtcac ccgtaagatc acccgcgcca tcgccaatat
     9241 cgcccaggga ctggagtcct gcctgtatct cggcaacatg gactcgctgc gcgactgggg
     9301 tcatgcgaaa gattacgtgc ggatgcagtg gatgatgtta cagcaggagc agccggaaga
     9361 tttcgtgatt gccaccggcg tgcagtactc cgtgcgccag tttgtggagc tggcggcggc
     9421 gcaactgggg ataaaattac gctttgaagg cgaaggcatt gatgagaaag gtatcgtggt
     9481 atccgttacc ggacacgatg cgccgggcgt gaaagcgggc gacgtgattg tggccgttga
     9541 tccgcgctat ttccgtccgg cggaagtgga aaccctgctg ggcgacccgt ccaaagcgca
     9601 tgagaaactg ggctggaaac cggaaatcgc cctgtcggag atggtctccg agatggtggc
     9661 gaatgatctg gaggccgcga aaaaacactc actgctgaaa tctcacggat tcaatgtaaa
     9721 tttatctttg gaatgaccat gaataagcaa cgaatttttg tggcgggcca tcgcgggatg
     9781 gtgggctccg ccattgtacg gcagcttgcg cagcgcggcg acgtggaact ggtgctgcgc
     9841 acccgcgatg agctggatct gctcgacggg cgcgcggtac aggcgttctt tgccggggcg
     9901 ggtatcgacc aggtttatct ggcggcggcg aaagtgggcg gcattgtcgc caacaacacg
     9961 tatccggcgg attttatcta tgaaaacatg atgatagaga gcaacattat tcacgccgcg
    10021 cacctgcaca acgtgaacaa actgctgttt ctcggttcgt cctgtatcta tccgaaactg
    10081 gcaaggcagc cgatggcgga aagcgagctg ctgcagggag cgctggagcc gaccaacgag
    10141 ccgtatgcca tcgccaagat cgccgggatt aaactgtgcg agtcctacaa ccggcagtac
    10201 ggccgcgact accgttcggt gatgccgacc aacctgtacg gcccgcatga caatttccat
    10261 ccggacaatt cacatgtgat cccggcgctg ctgcgccgct ttcatgaggc tgcgcagagc
    10321 catgcgccgg aggtggtggt gtggggcagc ggaacgccga tgcgtgaatt tctgcacgtt
    10381 gatgatatgg cggcggccag cattcatgtg atggagctgg cgcgcgaagt gtggcaggag
    10441 aataccgccc cgatgctgtc gcacattaac gtcggcaccg gcgtggactg caccatccgc
    10501 gagctggcgc agaccatcgc gaaggtggtg ggttaccagg gcagggtggt gttcgatgcc
    10561 gcgaagccgg acggcacgcc gcgtaaattg ctcgacgtca cgcgcctgca tcagcttggc
    10621 tggtatcacg aaatttcact ggaggcaggg cttgccggta cttaccagtg gttccttgag
    10681 aatcagcagc ggttccgggg gtgacaatgt ttttacgtca ggaagatttc gccgccgtgg
    10741 tacgaaccac gccccttatc tccctcgatt tcattgtgga aaacagccag gggaaaatcc
    10801 tgctgggcca gcgtctcaac cgtccggcgc agggctactg gtttgtgccg ggaggacggg
    10861 tgtgcaaaga cgaaacgctg gaggccgcct tcgaacgcct gacacaggcg gaactgggcg
    10921 tgcgtctgcc actggcggca gggacgtttt atggcgtctg gcagcacttc tatgacgaca
    10981 acttttccgg tgaggatttt tcaactcact atatcgtgct cggctttcgt ctgcgcgtgg
    11041 cacagggcga tttacacctg cctgacgccc agcatggcag ttaccgctgg ctgacgccgg
    11101 aacagcttct ggcaggcgat aatgttcatg agaacagtcg agcttatttt caaaatgaac
    11161 cccattctgt cattggttta gacaaaaagg acgtcaagta tgtctgatgt acccttaatt
    11221 gctgttgtaa tggctggtgg cactgggagc cgtctttggc ctctatctcg agaactttac
    11281 ccaaaacaat ttttacaact ttctggaaaa aattccttat tacagacaac cctgcttcgg
    11341 ctgtcggcac ttgcatgtaa tgaaccattg gttataacaa acgagcagca ccgttttatt
    11401 gttgcggagc agttacggca aatagatcaa ctacataata atatcattct tgaaccgtat
    11461 gggagaaata cagctccagc gattgctctt gctgcattat cagcgcttaa acgtaaggag
    11521 caggaggatc ctttactttt agttcttgca gccgatcata ttatcgctaa agaaaaagtt
    11581 ttctgtaatg ccgttaagaa agcagtcccc attgccgaaa acggcaatat tgttacattt
    11641 ggtatcatac ctgaatatgc tgagactggt tatggctata tagagcgtgg tgcacctttc
    11701 tctgtaaatg caaattcaac tgaaacagat ttttatcatg taaaaaattt tgttgaaaag
    11761 ccggatagat taaaagcaga gaaatatatt tcaacagggg attatttctg gaatagtggc
    11821 atatttatgt ttaaagccac tgtttatcta aatgagttaa aaaaattcag accagatatt
    11881 tatagcattt gtgagcaggt gctttcttct tcatatcgcg atcttgattt tattcgtctt
    11941 ccagaaactt tatttaaaga ttgccctgca gaatctattg attttgctgt tatggaaaaa
    12001 acggagcgtt gcattgtctg cccggttgac attggttgga gtgatgttgg ttcctggcag
    12061 tctttatggg atatcagcga aaaaacagat gagggagatg tttgtaaagg cgacattctg
    12121 acatacaaca ctaaaaataa ctacatatac tcagagtcag cattggttgc tgctgtaggt
    12181 gttgaagata ttgttattgt acaaacaaaa gatgccatac ttgtttctaa aaaatctgat
    12241 gtccaggatg taaaaaagat cgttgagatg ctaaaggcgc aagagcgtac agagtatatt
    12301 gcccatcgtg aggtttttcg tccatggggt aaattcgact caatagacca gggtaagcga
    12361 tataaagtta aaaagatcat cgtcaaacca ggagaagggc tttccttgcg tatgcatcat
    12421 catcgttcag aacactggat tgtgctttct ggtacggcta aagttacgct ggatgataaa
    12481 acgatgctag ttactgcaaa tgaatcaatt tatattcctt tgggggctac ttatagtctg
    12541 gagaatcctg gagttattcc acttaatctc atcgaagtta gttcaggaga ttatttaggt
    12601 gaagatgata tagtccggca gaaagaacgt tatagaaaag atgattgaat catgaataaa
    12661 ttaacctgtt tcaaggccta cgacattcgc ggcaggctgg gcgaagagct caatgaagat
    12721 atcgcgtggc ggattggccg cgcctgcggc gaatatttaa aaccgaaaac catcgtgctg
    12781 ggcggcgacg tgcgcctgac cagcgaggcg ctgaagctgg cgctggcgaa agggttacag
    12841 gacgcgggcg tcgacgtgct ggatatcggt atgtccggca ccgaagagat ttacttcgcc
    12901 accttccacc tcggcgtgga cggcggtatt gaagtgaccg ccagccataa tccgatggac
    12961 tacaacggta tgaagctggt gcgcgagggc gcccgcccga taagcggcga caccggtctg
    13021 cgcgacgttc agcgtctggc ggaagcgggc gatttcccgc cggtgaacga ggccgcgcgc
    13081 ggcagctaca gacaaatcac cctgcgcgac gcctatatcg accacctgct cggctacatc
    13141 agtgttaaaa atctcacccc gctgaagctg gtggtgaatt ccggcaatgg ggcggcgggg
    13201 ccggtcattg acgccatcga agcgcgtctg aaggcgctgg gcgcgccggt ggagttcatc
    13261 aaaatccaca acaccccgga cggcaccttc ccgaacggca ttcccaatcc gctgctgccg
    13321 gagtgccgcg acgacacccg caaggccgtt attgaacatg gggcggacat gggcatcgcc
    13381 tttgacggcg acttcgaccg ctgcttcctg ttcgatgaga aagggcagtt tatcgagggg
    13441 tactacatcg tcgggctgct ggcggaagcg tttctggaaa aacacccggg ggcgaagatt
    13501 atccacgacc cgcgcctgac caggaacacg gaggcagtgg tgacggcggc gggcgggacg
    13561 ccggtgatgt caaaaaccgg gcacgccttt atcaaggagc ggatgcgtac ggaggatgcc
    13621 atttatggcg gggaaatgag cgcgcatcac tattttcgtg attttgccta ctgcgacagc
    13681 gggatgatcc cgtggctgct ggtggcggag ctggtgtgcc tgaagggaca gtcgctgggt
    13741 gaactggtcc gcgaccggat ggcggcgttc ccggcgagcg gggaaatcaa cagcaggctg
    13801 gcggagccgg cagcggccat cgcgcgggtg gaagcgcact tcgcggaaga ggcgcaggcg
    13861 gtggatcgca cggacggcct cagcatgtca ttcccggact ggcgtttcaa cctgcgctcg
    13921 tcaaacaccg aaccggtggt acggctgaat gtggaatcgc gcggcaatga acaccttatg
    13981 caaagtaaaa ctgtcgaaat aataaaaatt ttaaattaac tttctaagtt tagggctatg
    14041 ccaagtacat agccttaact ttttcattta agattttatc agatggattg ttttttagaa
    14101 tgaaccatag tgtcgctata atactgagtg tttataaagc agaaaaaccg gcttatctgt
    14161 atcaaagtct ctccagcctg ttttctcaaa ctatgagtgc tgatatttat ctctatattg
    14221 acggagagat aactgtttca ctagaaaacg taattaacga ttttaaacct cgccctaatt
    14281 tttatattat tcgtggaaaa gataataaag ggctagcttt tgctctgaat agcttgatag
    14341 atataagttt acgtaatggc tacaaataca tagccagaat ggatactgac gatatatcac
    14401 gtttagatcg tattgaacag caatttagat ttatggagaa acggactgac gttgacgtgc
    14461 tgggtggata ttgtcatgaa tttggcagtg aatacgcgct tgaattaaag aaagtaccaa
    14521 ttgatcataa tagccttaaa aagtatagta taatatattg cccttttata catccaacgg
    14581 taatgttcag aagttcagtc tttaaggacg ggattcgcta tcctttagat acaaggtata
    14641 ctgaagatat agccttgtgg ctttgcctcc ttgataaggg atattgcttc cataaccttc
    14701 ccaaagtatt attagactat agaataaatg aggatacgtt ctcacgaaga agaggtttta
    14761 aaaaagcggt tagtgagttt tctgttagat ttaactatat gcggagcctc agaaatttta
    14821 gcattaagaa ttggtgtgtc ttaattgtaa gatttttatt ccacatgcta cctttgccat
    14881 tcattcgata tgcttataaa aaatatcgtt aaagcataat gtttataatt ttttatttgt
    14941 aagtttacca agaaatcgta agtagctaga ctggtgtctg tatatggatt taagaattag
    15001 tactaaatat tatcgtttcc ttgtaataat attttttgaa ttttaataaa tctagaatga
    15061 ataaaatgtg ttgaaatatc aactttttag gcaagatgat taaaaccatt gaacacttgg
    15121 tgtattattt ctatcattgt agctctgatc cgagctaata tctaagtcac atttcaagcc
    15181 gtgccctcgt cgcgcggtga ccacacctga caggagtaaa caatgtccaa gcaacagatc
    15241 ggcgtcgtcg gtatggcagt g
//
