LOCUS       JX975328               15667 bp    DNA     linear   BCT 27-AUG-2013
DEFINITION  Salmonella enterica strain G1454 O antigen gene cluster, complete
            sequence.
ACCESSION   JX975328
VERSION     JX975328.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Reeves,P.R. and Wang,L.
  TITLE     Structural diversity in Salmonella O antigens and its genetic basis
  JOURNAL   FEMS Microbiol. Rev. (2013) In press
   PUBMED   23848592
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15667)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Reeves,P.R. and
            Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1454"
                     /db_xref="taxon:28901"
                     /note="serogroup: O48"
     misc_feature    1..15667
                     /note="O antigen gene cluster"
     gene            118..744
                     /gene="nnaD"
     CDS             118..744
                     /gene="nnaD"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaD"
                     /protein_id="AFW04663.1"
                     /translation="MNMKQKLIIIGAGGFAKTVIDSLDHKKYEIKGFIDTFKTGEHQG
                     YPILGENLNIIYEPNQYVYFIAIGDPECRALWMNLLEEKQLSTINVIDRTSIISERSK
                     LGSCIYVGKMAIINCDSELEDGVVINTRALVEHGNYISYCTNVSTNVVLNGDVSVGTK
                     SFIGSCTVVNGQLKIGNSSIIGSGSVVIRDIPDNVVVAGSPTKFIRAR"
     gene            750..1790
                     /gene="nnaB"
     CDS             750..1790
                     /gene="nnaB"
                     /note="NnaB"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuNAc condensing enzyme"
                     /protein_id="AFW04664.1"
                     /translation="MSKVYIVAEIGCNHNGDFQLAQEMVKEAKKAGVDAVKFQTFKAD
                     KLISKYAPKAEYQIKVTGNEESQLEMTRKLELPYDEFINLEEYAKQLGLDVFSTPFDF
                     DSIDFLASRNQKVWKIPSGELLNLPYLEKIAQLPIADKKIVLSTGMATIEEINLSLKV
                     FSDNGISPENITILHCNTEYPTPFEDVNLNTICGFKKIFEQYNIGFSDHSPGYFAGIA
                     SVPYGITFIEKHFTLDKNLEGPDHKASVTPDELKLLCEGIRAVEVSLGSYVKLVTDSE
                     RKNKIVARKSIVAKCAIKKGEIFTIDNITTKRPGNGISPMHWYGVLGEKAEKDFEEDQ
                     LIVHSCFSEQEV"
     gene            1794..3059
                     /gene="nnaC"
     CDS             1794..3059
                     /gene="nnaC"
                     /note="NnaC"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-NeuNAc synthetase"
                     /protein_id="AFW04665.1"
                     /translation="MSLKKIAIIPARSGSKGLPNKNILMLLDRPLIAYTIEAAINSDV
                     FDRVVVSTDSLEYKSIAEKYGAEVIMRPEALSSDSATSFMVIEDVLNKCKGYHYFVLL
                     QPTSPFRNYKHVMSAVEQFEYADNAKFLVSVVESNKSSALIKPIDDSLSLRNFNCDFS
                     NYRRQNRKEYYPNGAIFIGYVADYLRRKHFFGEGSIAYIMNKEDSIDIDDQLDFEIAI
                     LIQNKKNKKTMLDNSIIKRISDKKDIFNNAAPITLIGHSIFDYWDIDNLCGLKVNNLG
                     IAGIDSEKYYRHILEKKLITSVGDYAVLISGTNDIVLEGWKAEDTITWTKKLIDGIKS
                     INPNVTIFLLAVPPVLGRIDRNNCIIKELNLLMQNYFSSLEYVVWVPLSTSFYDGFGN
                     LNKEYTYDGLHFTPQAYKQLENDISSILK"
     gene            3056..4222
                     /gene="nnaA"
     CDS             3056..4222
                     /gene="nnaA"
                     /note="NnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-2-epimerase"
                     /protein_id="AFW04666.1"
                     /translation="MKKKLLYVTGSRAEYGIMKRLLKSLNDDPNIELSIIATGMHCDP
                     EYGCTYKTIEQDGFSIEKLVDLKLENKTNAGILKSMSICQEAFGEYFQEHKYDAVIIL
                     GDRYEIFSVAVAAAMHNLPIIHLHGGEKTLGNYDEFIRHSITKMSRLHLVSTDEYRRR
                     VIQLGEEPEFVFNVGALGAENSFLLDLPDKVELEKKLGKLDRAYFVVVFHPETLSTLP
                     VNEQYLELLGALSDFSQEYDYIFIGSNADTGSEKIKEMTLDFCREYGCRYIVSMRTEE
                     YLALVKCSCGLIGNSSSGLIEVPSLKVPTINIGDRQKGRVRGYSVIDTICKKENIISA
                     IVSSRKECFINRLQCETNPYYNKDVNKNITKIIKTFLNIDCDKLGYKDFYDIYI"
     gene            4402..5646
                     /gene="wzx"
     CDS             4402..5646
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFW04667.1"
                     /translation="MFNTMLKYYTSVVLRGFTLLTKFIFIILLARFLQASDLGLYGLI
                     SAAVGYGIFVVGFEFYTYSTREIINSHKETLFFILKNQVLFYLISYTLCIPVFIILVY
                     LNILPRGSEYWFIALLFFEHLSQEINRVLITIESQTIASFILFVRQGLWCWLAIATMV
                     VYPDLRNITVVFIYWLIGTVFACVLGILYILNKKTGNNTIKWDWAWLKKGIRLSAPML
                     IAALALRGFFTFDRFAIEKISGLEILGGYTLFVSMTSAIQSFLDTILISFSFPKLARL
                     YSEQKYIEFKSELKKFALKLILLLSILSICCFFTGYILLKWLDNLNYIKLFPVFILLI
                     LATFVYCISLIPHIALYAMREDHCILMSQCISFLAFISFLIMSIYLSEIYCVIIGMVV
                     SFILLLLLKTLPLYNILNKVKC"
     gene            5648..6841
                     /gene="wzy"
     CDS             5648..6841
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFW04668.1"
                     /translation="MIIKKNKFVYSVLKVWLIVSSLYYLNAIFSGVDALKYNEDLTQK
                     FIKYAICFIISFFILFNSKRVKFLGASLFFIILSVASVVIGNVVTVYATAMLIIATMV
                     GFSQIILYFSNDMSKINMVLLWTGIIIGAISVLELTVFYDYMVSYWVSTGGVRSISSL
                     LNPTNSGAYSAIIILIALGTNIRSKFKRTLFALMPMITLISSGSRTAWLSLALTLLLT
                     VLLNDKASIRLRKKILAIAGIGAICGVLYVAFYMSSISGIQSQHRGLDTYTASIRVEN
                     FITYMNSIDFDMLLPDFLDKNINLISDNFYLVLLNYFGIIGGYIVFFISVLLFYYNMQ
                     TKDFDKNIDESISVWRVIFIYFLISGLSNSFINSFPVNQLFFISCGYYVYKYNLIKKN
                     AGKQI"
     gene            6838..8157
                     /gene="wbuW"
     CDS             6838..8157
                     /gene="wbuW"
                     /note="WbuW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04669.1"
                     /translation="MKLSMMQLLNCYLLATKNEIAIDEYLSKGISKKIPPLPRTSKWV
                     GEKWSSLTLLFIYVTLVVVFIFGGFFIYIILFLTRFYLCKLKQLTSKSIIFNDEDDRI
                     YYFAFTELGTNQVCHFFKEKNNREYSSNKITIVKLPWLNFEPINNNCDSVALYGFISF
                     FDVTKAFLLTIFSFVCFFKPSCIKWILHLYTAPSWFLIALAMNNVKGSFASSEHYDRW
                     AVLTDIVCKIKRKRYILIQHGSLLGLKAKNFEYFNLPYKLKSVSEIVVFNKIEFDLFS
                     DYILSKNKNDGLIVHYFQQPFIVSSIIEKKELSVLVVGHSLCERAQLKIGERLSTLSD
                     EIVVYYKEHPRARASEKVKRAAWNFITDDNYFPDVDLVISYPSTLALQYQDLNKTVLL
                     HGLDNINQDEINEIITTVIKSKDIYEKDNYQICRHCIMDTNRSEHHF"
     gene            8087..9229
                     /gene="wbuX"
     CDS             8087..9229
                     /gene="wbuX"
                     /note="WbuX"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="AFW04670.1"
                     /translation="MKKIIIRSVGIVLWIQTDPNIIFDDKGKCDYCTNYENNIFPAWS
                     KGSSSGCFLSEMANKIKTDAKNSDFDCIIGLSGGLDSSYAAYIAKEKMGLRPLLFHVD
                     AGWNTDQAVGNIEKLIEGLGLDLYTEVVNWEEMKDLQLSFLKSGIPDQDLVQDASFFS
                     ALYKFARQHRIKHVITGSNYSTECCREPEEWGGYLGIDTLLFNDIHKKFGTRQLKSFP
                     LVDILVYKLFYQKILGMKVHHPLNLVPFNKKNAENELKEKFGWQPFQHKHHESRFTRF
                     YEDYWLPRRFGFEKRRAHFSSLIMTGQMTREEALERISKPEMDEHFLKQEFEYVAHKL
                     GITVDELQQLFDMPKKTYRDYKNKRWLIGLGANVLRTLGLEKRYFR"
     gene            9226..9840
                     /gene="wbuY"
     CDS             9226..9840
                     /gene="wbuY"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuY"
                     /protein_id="AFW04671.1"
                     /translation="MIKIIDYGIGNIQAFLNVYKRLGISADVARSVNDIESATHLVLP
                     GVGAFDQAMALFNDSGLREGIEKRVFEERIPIIGICVGMQMLATSSEEGNMSGLGWIP
                     GQVRTFSSNIASKNLPMPHMGWNSINKRTNTLLLKDFEETPSFYFLHSFYYECDDVDD
                     VLATAEYGHNFHCIVGRNNIFGIQCHPEKSHSSGYQLLKNFAEI"
     gene            9845..10633
                     /gene="wbuZ"
     CDS             9845..10633
                     /gene="wbuZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuZ"
                     /protein_id="AFW04672.1"
                     /translation="MLRPRIIPCLLIHDSGLVKTINFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSKGREPDYDLIKKLAAECRMPLCYGGGVTTAAQAAKIISLGVEKVSI
                     STAAVENPNLVRELAEAVGKQSVVVVLDIIKRKGLFSKGYELSTRNNTRTHKIDPISF
                     AQEMAGLGAGEIVINYIDNDGVMNGYDIGYSSTIKSHVNVPVTFLGGAGSYEHLTELI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTPKQKDMICNGSMKNF"
     gene            10643..11680
                     /gene="fnlA"
     CDS             10643..11680
                     /gene="fnlA"
                     /note="FnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="4,6-dehydratase, 3- and 5-epimerase"
                     /protein_id="AFW04673.1"
                     /translation="MMFKDKTLLITGGTGSFGNAVLNRFLSTDIKEIRIFSRDEKKQD
                     DMRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNV
                     LGTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVNSNKTVI
                     CGTRYGNVMASRCSVIPLFVDLIKAGRPLTITDPNMTRFMMTLEDAVDLVLYAFEHGN
                     NGDIFVQKAPAATIDTLAIALKELLNVERHPVNVIGTRHGEKLYEALLSREEMIAAID
                     MGDYYRVPPDLRDLNYGKYVEQGDSRISVVEDYNSHNTQRLDVEGMKTLLLKLPFIRA
                     LCAGENYDLEA"
     gene            11682..12785
                     /gene="fnlB"
     CDS             11682..12785
                     /gene="fnlB"
                     /note="FnlB"
                     /codon_start=1
                     /transl_table=11
                     /product="reductase"
                     /protein_id="AFW04674.1"
                     /translation="MKILITGADGFIGRNLCLRLQEKGDVDLIKIDRDSSTIELEIGL
                     QEADFIYHLAGINRPKNIEEFIEGNRNLTQQIVDFLLVNQKKTPIMISSSIQAGLDNA
                     YGQSKAAAEKQIERYAAKSGAAYFIYRYPNVFGKWCKPNYNSFVATFCHNIANDVDIT
                     INDPAASVSLVYIDDVCTHALKLLTGTVDSGYKDVAPIYSTTVGEVAELIYRFKESRS
                     TLITEAVGTGFTRALYSTWLSYLPTDKFTYTVPFNGDSRGIFCEMLKTPSSGQFSFFT
                     AHPGITRGGHYHHTKNEKFLVIRGQARFKFEHVITGERYELNVSFDEFKIVETVPGWT
                     HDITNIGNDELVVMLWANEIFNRDEPDTIARAL"
     gene            12785..13915
                     /gene="fnlC"
     CDS             12785..13915
                     /gene="fnlC"
                     /note="FnlC"
                     /codon_start=1
                     /transl_table=11
                     /product="C-2 epimerase"
                     /protein_id="AFW04675.1"
                     /translation="MKKLKIMSVVGTRPEIIRLSRVLAKLDEHCEHILVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAATIGQVIIKVDEVLESEKPEAMLVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     AADRIIKTGSPMFEVLSYYMPQIDGSDVLSRLNLRAGEFFVVSAHREENVDSPKQLVK
                     LAKILNTIAEKYNLPVIVSTHPRTRNRINEQGIEFHPNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALEILATQ
                     PRGATRLLRQVSDYSMPNVSDKVVRIVHSYIDYVKRVVWKEY"
     gene            13915..15126
                     /gene="wbuB"
     CDS             13915..15126
                     /gene="wbuB"
                     /note="WbuB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04676.1"
                     /translation="MKLALIIDDYLPDSTRVGAKMFHELAQEFIQRGHDVTVIYPDSS
                     LQEKILFDSFQGVKTWRFKSGSLKDVSKIQRAINETLLSYRAWNAIKNQIKEETFDGV
                     VYYSPSIFWGHLVKKIKTRCQCPAYLILRDMFPQWVIDAGMLKAGSPIELYFRFFERS
                     SYRQANRIGLMSEKNLDIFRITHRGYPCEVLRNWASLTPTVLSRDYVPLRKRLGLEDK
                     VIFFYGGNIGHAQDMANLMRLTRSMAEHPQAHFLFIGQGDEVELINSLASEWGLSNFT
                     YLASVDQDEFKLILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHINGEDDKLYHSALLLLQDINVRRKLGMGSNTLLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            15113..15511
                     /gene="wbuC"
     CDS             15113..15511
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="AFW04677.1"
                     /translation="MRLIDNDQLESLYKQAAQSDRLRAHLLLHRSHQERVQRLLIALV
                     KGSYVEPHYHELPHQWEMFIVMAGQLQVCLHGKDGKVIKQFVVGDDSGISVVEFSPGD
                     IHSVLCMSQRALMLEVKEGPFDPSFAKAFI"
BASE COUNT     4934 a   2421 c   3156 g   5156 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgttttttag attactcata ttaatatcgt
       61 ttccaacgtt gcgaatagca aatagattaa ttaatataca acaaaccatc gaatggtatg
      121 aatatgaaac aaaaattaat aattattggc gcaggtggtt ttgctaagac tgtaatcgat
      181 agtttggatc ataaaaaata tgaaattaag ggatttatcg atacatttaa gacaggtgag
      241 catcagggtt acccaattct gggtgaaaac ttaaacataa tatatgagcc gaatcaatac
      301 gtctatttta ttgcaattgg agatcctgag tgcagagctt tatggatgaa tttattagaa
      361 gaaaagcaac tttcaaccat taatgtaatt gacagaacat caataatatc agagcgttct
      421 aagcttggca gttgtattta tgtcgggaaa atggccatta ttaattgtga ttcagagcta
      481 gaagatggtg tagttataaa tacaagggcg ctagtagagc atggtaatta tatatcctat
      541 tgtacaaatg tatctaccaa tgttgttcta aatggtgatg tatctgtggg gacaaaatcg
      601 tttattggca gttgtactgt tgttaatggg caacttaaga taggtaattc atcaataatt
      661 ggttccggat ctgtagtcat tcgcgatatt cctgacaatg ttgtagtggc aggatctccc
      721 actaaattta tcagagcaag gtaaagttta tgtcgaaggt ttatattgta gctgaaattg
      781 gatgtaatca taatggggac tttcaattag ctcaagaaat ggttaaagaa gcaaaaaaag
      841 caggtgttga tgctgtaaaa tttcaaacat ttaaagcaga taaacttatt tcaaaatatg
      901 cacccaaagc tgaatatcag attaaagtta caggtaatga agagtcgcaa ttagaaatga
      961 ctcgcaaatt agagttacca tacgatgaat ttataaatct agaagaatat gcaaaacaac
     1021 ttggattaga tgtgttctct acaccatttg attttgattc aatcgatttt cttgcatcaa
     1081 gaaatcaaaa agtatggaag ataccatcag gtgaattatt aaatttgcct tacttagaaa
     1141 aaattgcgca attacctatt gcggataaaa agattgtatt gtcaactggt atggcaacga
     1201 ttgaagagat taatttatcg ttaaaggttt tttctgataa tggtatttct cctgaaaaca
     1261 taacaatatt gcattgtaat acagaatatc caaccccttt tgaagatgta aatttaaata
     1321 caatttgtgg atttaaaaaa atatttgaac agtataatat tgggttttct gatcattcgc
     1381 ctggctattt tgcaggtatt gcatcagtgc cctatgggat cacgtttatc gagaagcact
     1441 tcactttaga taaaaacctt gaaggtccag atcataaagc atcagttact ccagatgaac
     1501 taaaattatt gtgcgagggg attcgtgcag tagaagtttc gcttggcagc tatgttaaac
     1561 tagtaactga ctcagaacgg aaaaataaaa tagtagctcg caaatcaatt gtcgctaaat
     1621 gtgcaataaa gaaaggggaa atttttacaa tagataatat cactacaaaa cggcctggca
     1681 atggaattag cccaatgcat tggtatgggg tcctggggga gaaggctgag aaagattttg
     1741 aagaggatca actaattgta cactcatgct ttagtgaaca agaggtttga gtcatgtcat
     1801 taaaaaaaat tgctattatt ccagccagat ctggctctaa aggattacct aataaaaata
     1861 tattaatgtt attggatcgt cccttaattg cttatactat tgaagcggct attaattctg
     1921 atgtgtttga cagagttgtt gtttcaactg attcattaga atataaaagt attgcagaaa
     1981 aatatggtgc agaagtaatc atgcgcccag aagcattatc atctgactca gcaacatctt
     2041 ttatggtaat tgaggatgta ttgaacaaat gtaaggggta tcattacttt gttcttctac
     2101 aaccaacgtc tccgttccgc aattataagc atgttatgag tgcagttgaa caatttgagt
     2161 acgctgataa tgctaaattt ttagtttctg ttgttgaaag caataaaagt tcagccttaa
     2221 ttaaaccaat tgatgattca ttatcattga gaaattttaa ttgcgatttt agcaactatc
     2281 gcaggcaaaa tagaaaagag tattacccaa atggagcaat ttttattggc tatgttgctg
     2341 attatttaag acgaaaacat ttctttgggg aaggaagtat tgcctacatt atgaataaag
     2401 aggattctat tgatatagat gatcaattag actttgaaat agccattcta attcaaaata
     2461 aaaaaaataa aaaaacaatg ctagataatt ctattattaa acgtatttct gataaaaaag
     2521 atatttttaa taatgctgca ccaataacac tgattggaca ttcaattttt gattattggg
     2581 atattgataa tttgtgtggg ttgaaagtta acaatttagg tatcgcgggt attgatagcg
     2641 aaaagtatta tagacatatc ttggagaaaa aattaattac cagtgttggt gattatgcag
     2701 tattgatttc aggtacaaat gatattgtcc ttgaagggtg gaaagctgaa gatacaataa
     2761 catggacaaa aaagctgata gatggtatta aatccataaa tcctaatgtg actatttttc
     2821 tattagctgt accgccagta cttggtagaa ttgatagaaa taattgtatt ataaaagaat
     2881 taaatttatt aatgcagaac tattttagca gtctagagta tgttgtttgg gttcctttat
     2941 ctacatcttt ttatgatgga ttcggtaatt tgaataaaga atatacttac gatggtttgc
     3001 attttacacc gcaagcatac aaacagctag aaaatgatat ttcgagtatc ttaaaatgaa
     3061 aaaaaaactt ctttatgtta ctggctctcg agctgaatac ggtattatga aaagactttt
     3121 aaaaagtctg aatgatgatc caaatattga gctatcaatc attgctacag gaatgcattg
     3181 tgatcctgaa tatggttgta catataaaac tattgaacaa gacggttttt caattgaaaa
     3241 actggttgat ttaaaattag agaataagac aaatgctggt atcttgaagt ctatgtcgat
     3301 atgtcaggag gcattcgggg agtattttca ggagcacaaa tatgacgctg tcattatatt
     3361 aggagaccgc tacgaaatat tttcagtagc agtggcagca gctatgcata atctacctat
     3421 tattcatctc catggcggtg aaaaaacact cggcaattat gatgaattta ttcggcattc
     3481 tatcacgaag atgagtcgcc tacatttagt atcaacagat gaatatcgaa gaagggttat
     3541 tcaacttggg gaagaacctg aattcgtatt taatgttggt gctctgggag cggaaaatag
     3601 ctttctgctt gatttgcctg ataaagtaga gttagagaag aagttaggaa aactggaccg
     3661 ggcgtatttt gttgttgtat tccatcctga aactttatca actcttccgg tcaacgaaca
     3721 gtacttagag ttattagggg ctttgagcga ttttagccaa gaatacgatt atatttttat
     3781 tggatcaaat gctgatactg gttctgagaa aataaaagaa atgacccttg atttttgtag
     3841 agaatatggt tgccgctaca ttgtttctat gagaacagaa gaatatttag cattggttaa
     3901 atgctcatgt ggactgattg gtaattcctc atctggtctc atagaagttc caagtttaaa
     3961 ggttcctaca ataaatatag gcgaccgcca aaaaggaagg gttcgaggat attcagtaat
     4021 agataccatt tgtaaaaagg agaatataat atcagcaatt gtttcctcaa gaaaagaatg
     4081 ctttattaac aggttgcaat gtgaaactaa tccttactat aataaagacg taaataaaaa
     4141 tataacaaaa attataaaaa cttttttgaa tatagattgc gataaattag gatacaaaga
     4201 tttttatgat atatatatat gactgcctgt gataatataa tacaatataa ggtaataatt
     4261 gtttttaaat aattctattt tttttgtttt catagtgctg gttagtattt aaaatgagat
     4321 caaaagcgta aaatatttta gatgatatga gtttaataac ggacgtttgg ggttttttat
     4381 cggtttttat attgactttg gatgtttaat actatgctga agtattacac aagtgttgta
     4441 ttgagaggtt ttacactact tactaaattt attttcataa ttttgttagc ccgatttttg
     4501 caagcttcag atttgggatt gtatggatta attagtgcag cagtaggata cggcattttc
     4561 gtcgtaggtt ttgaatttta tacttactca actcgagaaa ttataaattc tcataaagaa
     4621 accctttttt ttatcctaaa gaatcaagta ttattttatt taatatctta tacactttgt
     4681 attccagtgt ttattatttt ggtttatttg aatatcttac cgcgaggaag cgaatattgg
     4741 tttattgccc tcttgttttt cgagcattta tcacaggaaa taaatagagt attaatcaca
     4801 attgagagtc aaacgattgc aagttttatt ctcttcgtaa ggcagggttt atggtgttgg
     4861 ttagctatcg ctacgatggt agtttatcca gatttgagaa atataacagt tgtattcatt
     4921 tattggttaa tcggtacagt gttcgcttgt gtgctcggaa ttctttatat tttaaataaa
     4981 aaaacaggga ataataccat aaaatgggat tgggcttggc taaaaaaagg tatcagatta
     5041 tctgcgccaa tgttaattgc tgctcttgct ctcagaggct ttttcacttt tgatcgattt
     5101 gcgatagaaa aaatatcagg attagagatt ttaggcggtt acacattatt tgtgagtatg
     5161 acgtcagcaa ttcaatcatt tttagatact attctaattt ctttttcatt ccctaaatta
     5221 gcaagattat attcggaaca aaaatatatt gaatttaaat ctgagttaaa aaaattcgca
     5281 ttgaagttaa ttttattgct atctatactt agtatttgct gtttttttac aggatatatt
     5341 ttacttaaat ggctagataa ccttaattat attaagttgt tcccagtgtt tatcttatta
     5401 atactggcca cttttgtata ttgtataagt cttataccac atattgctct atatgctatg
     5461 cgagaggatc attgtatttt aatgagtcaa tgtatttcat ttttagcttt catatctttt
     5521 cttattatga gtatctatct aagtgagatt tattgtgtaa tcataggcat ggtagttagt
     5581 tttattctac ttttgctgtt aaaaacattg ccattatata acattttaaa taaggttaaa
     5641 tgttaaagtg attataaaga agaataaatt tgtttattcg gtacttaaag tctggttaat
     5701 agtctcttca ttgtattatt taaatgctat tttttccggt gttgatgcgt taaaatataa
     5761 tgaagatttg acgcaaaagt tcatcaaata tgcaatatgt tttataataa gtttttttat
     5821 tctgttcaat agtaaacgag tcaaattttt gggggcttcg ttatttttca taatcctttc
     5881 ggtagcttca gtagttattg gtaatgtagt aacggtatat gctaccgcga tgctgataat
     5941 tgctaccatg gttggtttca gccaaattat tctttatttt tctaatgata tgtcgaagat
     6001 taatatggtt ttattatgga ctggaattat aataggcgcc atttctgtat tagaattaac
     6061 agtattctac gattatatgg tctcatattg ggtttcgact ggtggagtcc gatcaatatc
     6121 ttctcttctt aatcctacaa atagcggggc ttattcagcg attataattt taattgcttt
     6181 agggactaat ataaggagta aatttaagag aactttattt gctttaatgc caatgattac
     6241 tttaatcagc agcggatcac gtacagcatg gttatcatta gcattaacat tattattgac
     6301 ggtattatta aatgataagg ctagtatacg attacggaaa aaaatattag ctattgcagg
     6361 tattggagct atttgtggtg tgttatatgt agcattttat atgagtagta tctctggtat
     6421 tcaatcccaa catcgagggc ttgatacata taccgcatca attcgagttg aaaactttat
     6481 aacatatatg aattcaattg attttgatat gttattacct gatttccttg ataaaaatat
     6541 taaccttata tcagataatt tttatctagt gttattaaat tattttggaa ttattggggg
     6601 ttatattgtt ttttttattt cagtattatt attctattat aatatgcaaa caaaggattt
     6661 tgataaaaat atagatgaaa gtatttctgt ttggagagtg atattcatct attttctgat
     6721 atctggactt tcaaactcat tcataaattc ttttcctgta aaccaactat tttttatctc
     6781 atgcgggtat tatgtgtata aatataactt aataaagaaa aatgctggaa agcaaatatg
     6841 aaactatcta tgatgcaatt attaaattgc tatttactgg caaccaaaaa cgaaattgct
     6901 atcgatgaat atttatcgaa ggggatttca aaaaaaatac caccattgcc aagaaccagc
     6961 aaatgggtcg gtgaaaaatg gagttcatta actttactat ttatttacgt tacgctcgta
     7021 gttgttttca tttttggtgg tttttttatt tatattattt tgttcttaac aagattttat
     7081 ttgtgcaaat tgaagcagtt aacttctaaa tctatcattt ttaatgatga agatgataga
     7141 atttattatt tcgcatttac cgaattagga acaaaccaag tttgccattt ttttaaggaa
     7201 aaaaataatc gtgaatactc atctaacaaa ataactatag ttaaattacc atggttgaat
     7261 tttgagccga ttaataacaa ctgtgatagt gtcgctttat atgggtttat ttcttttttt
     7321 gatgtgacaa aagcattttt gttaactatt tttagctttg tctgcttttt taaaccatcc
     7381 tgtatcaaat ggatacttca tctgtatact gcaccttcat ggtttttgat tgctttggca
     7441 atgaataatg ttaaaggtag tttcgctagt agtgagcact atgatcgttg ggctgtatta
     7501 acagatattg tatgtaagat aaaaagaaaa cgatatatat taattcaaca tggttcttta
     7561 ctaggactga aagcaaaaaa ttttgaatat tttaaccttc cctataaatt aaaatcagta
     7621 tctgaaattg tagtttttaa caagattgaa tttgatttat tttcagatta tattctttca
     7681 aaaaataaaa atgacggatt aatagtccat tattttcagc aaccattcat tgtatcatct
     7741 attattgaaa aaaaagaact atcggtacta gttgttgggc actctttatg tgagcgagca
     7801 caactgaaaa tcggtgagcg gctctcaaca ttatctgatg aaatcgttgt ctattataaa
     7861 gagcacccta gagcaagagc ttccgaaaaa gtaaaaagag cagcttggaa ttttatcact
     7921 gatgataatt attttcctga cgtggatctt gtcatttcat atccttcaac actggcattg
     7981 caatatcaag atttaaataa aacagttctt ctccatggat tagataatat taatcaagat
     8041 gaaatcaatg aaataattac aaccgttatt aagtctaagg atatatatga aaaagataat
     8101 tatcagatct gtaggcattg tattatggat acaaaccgat ccgaacatca tttttgatga
     8161 taaaggaaaa tgtgattatt gcactaatta tgaaaataat atatttcccg catggagcaa
     8221 aggttcttct tcgggatgtt ttttatctga aatggcaaat aaaataaaaa ctgatgcaaa
     8281 aaatagtgat tttgattgca ttattggttt gtcaggaggt ttggacagct cttatgctgc
     8341 atatattgcg aaggaaaaaa tgggattaag gcctctttta ttccatgttg atgcaggatg
     8401 gaatacagat caagctgtgg gtaatattga aaagcttatt gaagggcttg gtttagacct
     8461 ctatacagag gttgttaatt gggaggaaat gaaagattta cagctttcct ttttaaaatc
     8521 tggtattcct gatcaggatt tagttcagga tgcatccttt ttttctgcct tatataaatt
     8581 tgctcgccaa catagaatta aacatgtcat tacaggttct aattattcga cagaatgttg
     8641 tcgtgaacca gaggaatggg gcgggtattt aggcatcgat accttattat ttaatgatat
     8701 tcataaaaaa tttggtacgc gacagttaaa atcatttcca ttagttgata tattagtata
     8761 caaactcttc taccagaaaa ttcttggtat gaaagtgcat cacccactta acttagttcc
     8821 ttttaataaa aagaatgcag aaaatgaact gaaagagaaa tttggttggc aacccttcca
     8881 gcataaacat catgaatctc gttttactcg tttctatgaa gattattggt tgccacgtag
     8941 atttggtttc gagaaacgaa gagcgcattt ttcaagttta attatgaccg gtcaaatgac
     9001 cagggaagaa gcattagaga gaatttcaaa acctgaaatg gatgaacatt ttcttaaaca
     9061 ggaatttgag tatgtagcgc ataaactcgg tattactgta gatgaattgc aacaactttt
     9121 tgatatgcct aaaaaaacct acagagatta caaaaataaa cgttggctaa taggtcttgg
     9181 tgcaaacgtg ctgcgtactc ttggattaga aaaaaggtat tttagatgat taaaatcatt
     9241 gattatggca taggaaatat acaagcgttc ctgaacgtat ataaacgact tggtattagt
     9301 gcggatgttg ctcgttccgt taatgacatt gaaagtgcta ctcatttagt attgcctggc
     9361 gttggggctt ttgatcaggc catggcgctt tttaatgact caggtttgcg tgaaggcatt
     9421 gaaaaacgcg tctttgagga gcgaataccc attattggta tttgtgtagg catgcagatg
     9481 ctagccacct caagtgaaga gggaaacatg tctggtttag gctggattcc tggtcaggtt
     9541 cgcacattca gcagcaatat cgcttcaaaa aatcttccaa tgccacatat gggatggaat
     9601 agtataaata aaagaacaaa tacactattg ctgaaagatt ttgaagaaac accttccttt
     9661 tatttcttac attcattcta ttacgaatgt gacgatgtag atgatgtatt ggcaactgct
     9721 gaatatggtc ataattttca ttgcatcgtt ggaaggaata atatttttgg catccagtgc
     9781 catccagaaa aaagtcattc ttcaggttac caactattga aaaattttgc agagatctaa
     9841 taatatgctt aggccaagaa ttataccctg tttgcttatc cacgattctg gtttagtcaa
     9901 aacaattaat tttaagtccc ctaagtatgt aggcgaccca attaatgccg tcaaaatttt
     9961 taatgaaaaa gaagctgacg aattgatggt ccttgatata gatgcaacat ccaaggggcg
    10021 tgagcctgat tatgatctaa ttaaaaaact tgcggctgaa tgccgtatgc ctttgtgtta
    10081 tggcggaggg gttaccactg ctgcccaggc agctaaaatt atctctcttg gcgttgaaaa
    10141 ggtatcaata agtactgcgg cggttgaaaa tccgaacctt gtccgtgagt tggcagaagc
    10201 agttggtaaa caaagcgtag ttgtagttct ggatattatc aaacgaaaag gtcttttttc
    10261 aaagggatat gagctttcaa cgaggaataa cactcgtacg cataaaatcg atcctatctc
    10321 atttgctcaa gaaatggctg gcttgggggc aggggaaatt gttatcaatt atatagataa
    10381 tgatggtgtc atgaatggtt atgatattgg atactcttct actataaaat ctcatgtgaa
    10441 cgttcctgtt acgtttctcg gtggtgcagg tagttatgag caccttaccg aattgattga
    10501 ccaatgcggt atcgtcggag cagcagctgg aagtcttttt gtatttaaag gtaagtatag
    10561 agcggtcttg ataagctatc ctacgcctaa acaaaaagat atgatttgta atggctcgat
    10621 gaagaatttt taataaggat ttatgatgtt taaagataaa acactgctga ttacgggtgg
    10681 aacagggtct tttggtaatg ctgtattgaa tcgctttctt tctacagata taaaagaaat
    10741 ccgcattttc tctcgcgatg aaaagaaaca agatgatatg cggaaaaaat ataataatca
    10801 gaagttaaag ttttatatcg gtgatgtcag agattatcgt agcattttga atgcaactcg
    10861 tggtgttgat tttatatacc atgcagcggc gcttaagcag gtgccatcat gtgaattcca
    10921 tccaatggaa gctgttaaaa ctaatgtgct cggcacagag aacgtactcg aagctgctat
    10981 cgctaatgaa gtaaaacgag ttgtttgctt aagtactgac aaagcagttt atcctatcaa
    11041 tgccatggga atttcaaagg caatgatgga gaaagtgatg gtcgcaaaat cacgtaacgt
    11101 aaatagtaat aaaacggtga tctgtggtac acgctatggc aacgtgatgg cttctcgttg
    11161 ttcggtgatt cctttatttg ttgatctcat aaaagcgggt aggccactga caattaccga
    11221 tccaaacatg actcgcttta tgatgactct tgaagatgca gttgatcttg tactttatgc
    11281 atttgaacat ggtaataatg gtgatatctt cgtacagaag gcccctgctg ctacgattga
    11341 tacattagca attgcactga aagaactctt gaacgttgag cgtcatccag ttaatgttat
    11401 cggtactcgc catggcgaga agttatatga agctttactt agtcgcgaag agatgattgc
    11461 agctattgat atgggggatt attaccgtgt tccaccagat ctgcgtgatc ttaattacgg
    11521 gaaatatgta gaacagggtg atagtcgtat ctcggttgtt gaagattata actcccataa
    11581 tacacagcga ctggatgtag aaggaatgaa aactctgctt cttaaattac cgtttattcg
    11641 cgcactgtgt gcaggtgaaa attacgatct ggaagcctga tatgaaaatc ctgattactg
    11701 gtgctgatgg ttttattgga cgtaacttgt gcttgcgtct tcaggaaaag ggggatgttg
    11761 accttattaa gattgatcgt gattcaagta ctattgagct tgaaataggc ctgcaagaag
    11821 ccgattttat ttatcatctc gctggtatca atcgacctaa aaatattgaa gagtttattg
    11881 aaggtaacag aaatctgact caacagattg ttgattttct gttagtgaat cagaaaaaaa
    11941 ctcctattat gattagttcc tctatccagg cagggctaga taatgcatat ggtcagagta
    12001 aagcagcagc tgaaaaacag attgaacgtt atgcggcaaa aagtggagct gcatatttta
    12061 tctaccggta tccaaatgtt ttcggaaaat ggtgcaaacc aaactataac tcgttcgtag
    12121 ctactttttg tcataacatt gccaatgatg ttgacattac catcaatgac ccagcagcat
    12181 cggtaagctt ggtttacatc gatgacgttt gtactcatgc gttaaagctt cttacaggaa
    12241 cggttgatag cggatataaa gacgttgctc cgatttattc aacaacagtt ggggaggtag
    12301 cagagttaat ttatcgcttc aaagaaagtc gttctactct tatcactgaa gctgttggca
    12361 ctggattcac tcgtgcattg tattcgacgt ggttaagcta tttgcctacc gacaagttta
    12421 cttatacagt gccattcaat ggtgatagcc gtggtatctt ttgtgaaatg ttaaaaacac
    12481 cgtcatcggg gcagttttcc tttttcacag cacatccagg gatcacgcga ggtgggcatt
    12541 atcaccacac taaaaatgaa aagtttctcg tcattcgtgg gcaggccaga ttcaaatttg
    12601 agcacgttat taccggtgag cgttatgaac taaacgtttc attcgatgaa tttaaaattg
    12661 ttgaaacagt tcctggttgg acacatgata tcacgaatat tggcaatgat gaattggtag
    12721 ttatgctctg ggcaaacgaa attttcaatc gtgatgagcc tgatactatt gcgagagctc
    12781 tataatgaaa aaattaaaaa ttatgtctgt ggttgggact cgtccggaaa ttattcggtt
    12841 atcacgcgtt ttggctaaac ttgatgagca ttgcgagcat atacttgttc atactggtca
    12901 aaactacgat tatgaattaa atgaagtatt ctttaacgac cttggtgtcc gcaagccaga
    12961 ttatttttta aacgccgcag ggaaaaatgc agctgcgacc attggccagg ttatcattaa
    13021 agttgatgag gtattagaat ccgaaaagcc tgaagcaatg ttggtgctgg gcgatacaaa
    13081 ttcctgtatt tcagctatcc ctgccaagcg tcgtaaggtg cctattttcc atatggaagc
    13141 tgggaaccgt tgcttcgatc agcgcgtacc tgaggaaact aaccgacgca ttgtcgacca
    13201 tactgccgac atcaatatga cctatagcga tattgcacgt gaatatctat tggctgaagg
    13261 gatcgcagcg gaccggatca ttaaaaccgg tagtcctatg tttgaggtac tttcttacta
    13321 catgccccaa atcgatggtt cagatgttct ctctcgcttg aatctacgtg ccggtgaatt
    13381 ttttgttgtg agtgcgcatc gagaagaaaa tgtcgattct ccgaagcaac tcgtcaagct
    13441 tgcaaagatt ctaaacacta ttgctgaaaa atataatctt ccggttatcg tttctacaca
    13501 tccgcggacg cgtaatcgta ttaatgagca agggattgaa ttccacccaa atattaatct
    13561 actgaagccg ttgggtttcc acgattacaa ccacttgcag aagaattctc gtgctgtatt
    13621 gtcagacagc ggtacgatca cagaagaatc atccatcatg aattttccag cggtaaacat
    13681 tcgggaagct catgagcgcc cagagggctt tgaggaagca tctgtcatga tggtggggct
    13741 agaatgcgaa cgtgtacttc aggctttgga gattctggca acgcagcctc gtggagcgac
    13801 ccgtctttta cgacaagtca gtgactacag tatgccgaat gtatcggata aagttgttag
    13861 aatcgttcac tcttatatag attacgtcaa gagagttgtc tggaaagaat attgatgaaa
    13921 cttgctctaa tcatagatga ttacctacca gatagtaccc gtgttggcgc aaaaatgttt
    13981 catgaactgg cccaagaatt tattcagcgt gggcatgatg ttacggtgat ttatcctgat
    14041 agtagtctgc aagaaaagat tttgtttgac tcgtttcagg gggttaaaac gtggcgcttc
    14101 aaaagcgggt ccctcaagga tgtgagcaaa attcagagag ccatcaatga gacgcttttg
    14161 tcgtatcgag catggaatgc tatcaaaaac cagataaaag aagaaacttt tgatggcgtg
    14221 gtttattatt caccttccat attttggggg catctggtta agaaaattaa aacccgttgc
    14281 caatgcccgg cttatctcat tttgagggac atgttccctc aatgggtgat tgatgcagga
    14341 atgctgaaag cgggttcccc aattgagctt tattttcgtt tcttcgaaag atcatcctat
    14401 cgtcaggcaa accgtattgg gctcatgtct gaaaagaatc ttgatatttt tcggataact
    14461 cacagaggtt atccttgcga ggttctacgt aactgggcat cacttacgcc gacggtattg
    14521 tcccgggatt atgtgccatt gcgtaagcgt cttggccttg aagataaagt tattttcttc
    14581 tatggcggaa atataggtca tgcccaggat atggcgaatc tgatgcgtct tacgcgaagt
    14641 atggcagaac atccgcaagc tcattttctg tttattggtc agggggacga agtcgaatta
    14701 atcaactctt tagcttctga gtgggggcta tcgaatttta cgtatttggc ctctgtggat
    14761 caggatgaat ttaagctaat tctgtcggaa atggatatag gcctgttttc actttccgcc
    14821 agacactctt cccacaattt ccctggtaag ttattaggct acatggttca gtcgctacct
    14881 atattaggta gcgtgaatgc cggaaatgat ttgcttgata tcgttaatca aaataatgca
    14941 gggctaatac atattaatgg tgaagatgat aagctttatc attccgcact gttattgctt
    15001 caagatatta atgtacgacg taagcttggg atgggctcga atacattatt gaaagaacag
    15061 ttctctgttg aatctgctgc acagacgata gaaatgaggc tggaggcatg taatgcgatt
    15121 aattgataat gaccagctcg agtcattata taaacaggca gcgcaatcag atcgcctgcg
    15181 tgctcacctt ttgttgcatc gttctcatca ggagagggta caacgactgc ttattgcttt
    15241 ggtcaagggg agctatgtag aacctcacta ccatgaacta cctcatcagt gggaaatgtt
    15301 tatcgtcatg gcagggcagc ttcaggtctg cttgcatgga aaagatggaa aggtcattaa
    15361 gcaatttgtt gtcggagacg atagcggaat aagtgtagtg gagttttctc caggggacat
    15421 acatagcgtt ttatgcatgt ctcaacgggc acttatgttg gaagtgaaag agggaccttt
    15481 tgacccatct tttgctaaag cgtttattta atgccggttt gaaacgcaat cttccgctat
    15541 ctaccgaggt caattctgag ttaacatcta agccacattt caagctgcgc cctcgtcgcg
    15601 cggtgaccac acctgacagg agtatgtaat gtccaagcaa cagatcggcg tcgtcggtat
    15661 ggcagtg
//
