LOCUS       JX975343               13117 bp    DNA     linear   BCT 27-AUG-2013
DEFINITION  Salmonella enterica strain G2470 O antigen gene cluster, complete
            sequence.
ACCESSION   JX975343
VERSION     JX975343.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Reeves,P.R. and Wang,L.
  TITLE     Structural diversity in Salmonella O antigens and its genetic basis
  JOURNAL   FEMS Microbiol. Rev. (2013) In press
   PUBMED   23848592
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Reeves,P.R. and
            Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G2470"
                     /db_xref="taxon:28901"
                     /note="serogroup: O53"
     misc_feature    1..13117
                     /note="O antigen gene cluster"
     gene            106..1191
                     /gene="rmlB"
     CDS             106..1191
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFW04832.1"
                     /translation="MKILITGGAGFIGSAVVRHIIKNTQDTVVNIDKLTYAGNLESLS
                     DISESNRYNFEHADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEVARKYWSALGEDKKNSFRFHHISTDEVYGDLPHPDEVGNSVTLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KSLPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNLDVVFTICDL
                     LDEIVPKANSYREQITYVADRPGHDRRYAIDADKISRELGWKPLENFESGIRKTVEWY
                     LANTQWVNNVKSGAYQSWIEQNYEGRQ"
     gene            1191..2090
                     /gene="rmlD"
     CDS             1191..2090
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AFW04833.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPVGNLIALDVHSKEFCGDFSNPK
                     GVAETVRKLRPDVIVNAAAHTAVDKAESEPELAQLLNATSVEAIAKAANETGAWVVHY
                     STDYVFPGTGDIPWQETDITSPLNVYGKTKLAGEKALQCNCPKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKERQTLSVINDQYGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAG
                     GTTTWHDYAALVFDEARKAGITLALTELNAVPTSAYPTPASRPGNSRLNTEKFQRNFG
                     LILPQWELGVKRMLTEMFTMTTI"
     gene            2132..3007
                     /gene="rmlA"
     CDS             2132..3007
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFW04834.1"
                     /translation="MEMKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLS
                     TLMLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQLSPDGLAQAFIIGEEFI
                     GNDDCALVLGDNIFYGHDLPKLMDAAVNKEDGATVFAYHVNDPERYGVVEFDKNGTAV
                     SLEEKPLQPKSNYAVTGLYFYDNSVVAMAKNLKPSARGELEITDINRIYMEQGRLSVA
                     MMGRGYAWLDTGTHQSLIEASNFIATIEERQGLKVACLEEIAYRKNYISKEKIQNAIL
                     KFGKSAYGAYLKSLF"
     gene            3154..4389
                     /gene="wzx"
     CDS             3154..4389
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFW04835.1"
                     /translation="MLRKNIFYLFIVQGGNYLIPLLTFPYLLRVLGPTSFGLLGFFQA
                     TIQYCILLIEYGFNWTATEAIAKNKNSKEKVTIIFWGVFWAKVFLASTSILLLLILIM
                     VFDIYAQHFLVILAFIPLIVSNIIYPVWFFQGIEEMKWITICTLLAKLIIVPTTFIFV
                     NNPNDIWVAAFIQSCSMMLSGLISVYIIMRKKWVSKIIFDYVYIRKCLSDGWHLFIST
                     SAISLYTTSTTIILGAIAGPIAVGYYNTANTIRGAMQGLLNPIAQAIYPRISYLITED
                     YQSALFLIKRMLKYFCCIALLSSLSLFIYAAPIIKIVAGTGYDGAVIVLRWMSFIPFF
                     VAMSNIFGVQTMLTHGYKKEFSFVLIISGVLNILLILPLVYFESQAGAAMALLLTEVF
                     VTAMMYIFLRKKKIFLFKR"
     gene            4396..5499
                     /gene="glf"
     CDS             4396..5499
                     /gene="glf"
                     /note="Glf"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="AFW04836.1"
                     /translation="MYDYLIVGSGFYGAVCAHELYKSGKKVLVVEKREHLGGNAFTEN
                     VKGIQVHKYGAHIFHTNDKLIWDYINKFSEFNRFTNSPIAIYKDKIYNLPFNMNTFYQ
                     LWGAKTPQDALEIINAQRADYLHQTPMNLEEKAISLVGKDIYEILIKGYTEKQWGRNA
                     TELPSFIINRLPVRFSFDNNYFSDRYQGIPIGGYTKIFEKLLSGIDIEFGVDYLRNKV
                     KYNSLAKKVIYTGAIDEYYDYIYGELEYRSLKFEHIILDLENYQGNAVVNYTDIAIPY
                     TRIVEHKHFDFIESSHTVITREYPSAWKKGDDAYYPINDKKNMDLFKRYRALATEEKN
                     IHFGGRLAEYKYYDMHQVIKAALNFVENSFRES"
     gene            5504..6622
                     /gene="wzy"
     CDS             5504..6622
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFW04837.1"
                     /translation="MSYLYLLFSGISLTSKLGVIVFWLSIPVIFFYCFGVSLLYNRKV
                     HKKYFIIVIWSVFFILMAGLSGYFLDDFNSRVISNVYLSLVGLFMALVFYNRGNSSND
                     KSFLTAIKITISINLCAFYIQFVSFYLFHYIIDYNLLSGGLGGRFYWGDLFRAAGFFD
                     EPAVYSLHMVALTVMLYMQERKFSILIIVTLLSLLLSFSFIAIFQAMILSLLFIKNKR
                     GLLVPLVVCLVVALLFKDNIYERYLQFVSGGDGSNNTKLDTIYNLFQGVKWLYGYGLV
                     GYSQSFPLYYQGLYDLTFWGANISLYGIVLGLIINVTVLFFLLKFTMRDFLLINVVLL
                     KLSTPTYGIYFCFLFFLLWYKQNMVIRDAKNISFNGDK"
     gene            6588..7388
                     /gene="wdbQ"
     CDS             6588..7388
                     /gene="wdbQ"
                     /note="WdbQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04838.1"
                     /translation="MPKISVLMAINKKTPYLDKAINSILNQTFKEFEFIIIANGCDDD
                     LYYLLNAYSRKDSRIILHRTELGGFAFALNYGLNLATTEYIARMDGDDLCTIDRLQKQ
                     YDIIKNNKSLSLLGTACNFIGPNDEEILSKKFKILTDNGAIRRALPYRNPIIHASIIC
                     RKDVLLSAGGYRYGHMSEDHELFIRLARDKNVIFMNTIEPLYSYRRHHMQVTDLSKSK
                     ESYAQISAFLFSEFLLTHNYKYIIGILRVHPYIRKMHNFFKRKFHNAK"
     gene            7378..8541
                     /gene="wdbR"
     CDS             7378..8541
                     /gene="wdbR"
                     /note="WdbR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04839.1"
                     /translation="MQNKIYHVCSGIRPGGGPAGYLYNLNEASKATKHNFIVDASNES
                     KIRSNTSYRKIKKLPNFIIKNALCMKYILALMGFTKISNEKNIFNFKYNLGHSVPVAA
                     RLLKLNKNKTVKIGLMPHGPVSYTTETLDDVESKYGKIYFRGFYFRIMSLLENNIFNK
                     VDFITVAAREGMESYQDINIQYEYKLFEVTSGLPPLICNTNKSDARKLLGIPDDKFIV
                     GFFGRYNRHKGYDFFYDEVISSANEKVFFISAGIGPIIKKEVHNYKNFGWRSDINNLI
                     TACDIIVIPNRHTYFDLLPLECFSLSRPVAVSLIGGNKKLCSLTQAAIPFKLNCGELN
                     NLVNNLSNNKTDYKKLESLAKKSYDELFSDKVFVTNHNSLLSSLNDFFCKEVE"
     gene            8538..9074
                     /gene="rmlC"
     CDS             8538..9074
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /protein_id="AFW04840.1"
                     /translation="MKFIKCTLNDVKIIYPTVYHDKRGFFLEGFNEKKYQELLGVSGF
                     VQDNISFSMKNVLRGMHFQKRFPQGKLVQVLYGSIYDVIVDLRKDSSTYLMWEGFHLD
                     AREHAQLWVPPGFAHGFLTLSDSVYFTYKCTEYYRPLDECCLLWSDPEINIDWPIPNG
                     ELIVSEKDANGLFIKDFM"
     gene            9092..9868
                     /gene="wdaC"
     CDS             9092..9868
                     /gene="wdaC"
                     /note="WdaC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04841.1"
                     /translation="MAIYVSIISHGHEDMLANTPLLNELSQLATVIIKNNKKSSKGTL
                     YNLKVNDNIIILDENDYGLGFGHNNNYVFKYAIENLGLNGNDYFCTINPDIIVDKLTV
                     SNVIEEMHSRNYELSTLFLYLDDNKKIKDESLRNFPVFIDFLKSYFLGKRDKVKLEKI
                     DINAYAVDWAAGSFLIFKASLFMKLKGFDQRYFMYCEDIDICYRAKKMGIRLICLTNY
                     SAIHLAQRASRTIFSRNFLWHLKSIIIYTLATNKKVKSIL"
     gene            10962..11990
                     /gene="gne"
     CDS             10962..11990
                     /gene="gne"
                     /note="Gne"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-GlcNAc-4-epimerase"
                     /protein_id="AFW04842.1"
                     /translation="MTILVTGGAGYIGSHTVLCLLENNYNVVVLDNFINSSEQSLKRV
                     SSLANRDVKIYNGDIRNRILLNNIFSEQNITHVIHFAGLKSVSESVNEPINYYDNNLN
                     GSLILVDMMLKYKIYNLLFSSSATVYGTPTSVPVNEKSHVGGTTNPYGTSKLMIEKVL
                     GDIATVEPKFRVTTLRYFNPVGAHCSGLIGEDPNGVPNNLMPYICQVAIGKQKQLLIY
                     GNDYPTKDGTGIRDFVHVMDLAEGHLAALRHSDEGPNYKVYNLGTGIGYSVLEILETF
                     QRITSKYVPRVFVDRRHGDIAECWSDPSRANKELCWKAKRGLEEMVRDAWNWQQRNPN
                     GYTKSNNN"
BASE COUNT     4191 a   1917 c   2562 g   4447 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgtgtaacga cttgagtact taatttttat
       61 tggcaaatta aataccacat taaatacgcc ttatggaata gaaaagtgaa gatacttatt
      121 actggcgggg caggttttat tggatcagct gttgttcgcc atattattaa gaatacacag
      181 gacactgtag ttaatattga taaattaacc tacgccggta atcttgaatc cctttctgat
      241 atttctgaaa gtaatcgcta caattttgaa cacgcggata tttgtgattc cgctgaaata
      301 acgcgtattt ttgagcagta ccagccggac gcggtgatgc atttggctgc ggaaagtcat
      361 gtggaccgtt cgattaccgg gccggcagct tttattgaaa ccaatatcgt cggtacttat
      421 gttcttcttg aagttgcgcg taaatattgg tctgcgcttg gtgaagataa aaaaaatagt
      481 ttccgctttc atcatatttc aactgatgaa gtttacggcg atttaccaca tcctgatgaa
      541 gttgggaata gcgttacgct gccgttattt actgaaacga cggcatatgc gccaagtagc
      601 ccctactctg cttcaaaagc atccagcgat catttagttc gtgcctggag gcgtacttat
      661 ggcctgccaa caatcgttac caattgttct aataactatg gtccttatca tttccccgaa
      721 aaactgattc cgttggtcat tttgaatgca ctggaaggaa aatctttgcc aatttatggc
      781 aaaggggatc agattcgcga ctggctatat gtagaagatc acgctcgcgc gctttatatt
      841 gtagtgactg aaggtaaggc aggggagact tataacattg gtggacacaa tgagaagaaa
      901 aatctcgatg tggtatttac catctgtgat cttctggatg agattgtacc caaagcgaat
      961 tcttatcgtg aacaaatcac ttatgtcgcg gatcgtccag gccatgatcg tcgttatgcc
     1021 attgatgcag ataaaattag tcgcgaatta ggctggaaac cgctggagaa ttttgaaagc
     1081 ggcattcgta aaacagtgga atggtacctt gcaaatactc aatgggtaaa caatgttaaa
     1141 agtggggcgt atcagagttg gattgaacag aactatgaag gacgccagta atgaatatct
     1201 tactttttgg taagacaggc caggtaggct gggagttgca acgttctcta gcgccagtgg
     1261 ggaatctgat cgccctggat gtccattcaa aagagttttg cggtgatttt agcaatccca
     1321 aaggcgttgc cgaaaccgtt cgtaagcttc gtcccgatgt gattgttaac gcagcagcac
     1381 atactgcagt agataaagca gagtctgaac cagaactagc gcagttactt aacgccacca
     1441 gtgtggaagc catcgctaaa gcggctaacg aaactggcgc atgggtagtg cattattcaa
     1501 ctgattatgt attccctggt accggcgata tcccatggca ggaaacggac atcacgtcgc
     1561 cgctaaatgt ctatggcaag accaaattag cgggagaaaa ggccctgcag tgtaactgcc
     1621 ctaaacacct tatcttccgc accagttggg tttatgcagg taagggcaat aatttcgcaa
     1681 agactatgct tcgtttggcg aaagagcgtc agacactttc ggtcatcaac gatcagtacg
     1741 gtgcgccaac cggtgcggaa ttactggctg actgcacggc tcatgcgatc cgtgtggcgt
     1801 taaagaaacc agaagtagca ggtctttacc atctggtggc cggtggaacc acaacctggc
     1861 atgactacgc tgcgttagtc tttgacgagg cgcgtaaggc agggataacg cttgcgctta
     1921 ctgagcttaa tgccgtgcca accagtgcct atccgacgcc ggcgagcaga cctggaaatt
     1981 cgcgtctcaa tactgaaaag tttcagcgta attttggcct tattcttccg caatgggaat
     2041 taggggttaa gcgtatgctg actgaaatgt ttacgatgac aaccatctga taaatttaaa
     2101 tgcccataag ggcattttct gtgaatgaga aatggaaatg aaaacgcgta agggcattat
     2161 tttagcgggg ggctccggca ctcgtcttta tcctgtgacc atggcggtaa gtaagcaatt
     2221 gctgccaatc tatgataaac cgatgattta ctatcccctt tccacgctta tgctggcagg
     2281 cattcgggat atcttgatca tcagtacgcc acaggatacg ccgcgttttc aacaactgct
     2341 gggagacggc agccagtggg ggttgaatct tcaatataaa gtacaactaa gtccggatgg
     2401 cttagcacag gcgtttatta ttggtgaaga gttcattggt aacgatgact gtgcattagt
     2461 gctgggtgac aatatcttct atggtcacga tttgcctaaa ctaatggacg ctgccgttaa
     2521 taaagaagat ggcgctaccg tctttgctta tcatgtaaac gatccggagc gctacggtgt
     2581 ggttgagttt gacaaaaatg gcacagccgt tagtctggaa gaaaaaccgt tacaaccgaa
     2641 gagcaattat gcggtaacgg gtctttattt ttacgataat agcgtggtgg cgatggcgaa
     2701 aaatcttaag ccttccgctc gcggtgagtt agagatcacg gatattaacc gtatttatat
     2761 ggagcaggga cgcttgtcag ttgcgatgat ggggcgcggc tatgcctggc tggatacggg
     2821 tacacatcag agtttgatag aggccagtaa ttttattgcg acgattgagg agcggcaagg
     2881 tttaaaagtt gcctgtttag aagagatagc atataggaaa aactatattt caaaagaaaa
     2941 aattcagaat gccattttaa aattcgggaa aagcgcatat ggagcctatt taaaatcttt
     3001 attttagtgg cgttattgtc ttactccagg gtatatcaaa gtacgttaaa gtaatgatgc
     3061 gatgataaga gtagataagc atagatattc ttttctcaag ctaatcatac aacttaattt
     3121 atattgaaaa tcaaaccaat atggtagcgc caggtgctta ggaaaaatat tttttacctt
     3181 tttattgttc agggcggaaa ttatcttata ccgctattaa cttttccata tttgcttagg
     3241 gttttggggc cgacttcttt tggtttgctc ggtttttttc aagcaactat tcaatattgt
     3301 atattactca ttgaatatgg ttttaattgg accgcaacag aagctatagc taagaataaa
     3361 aactcgaaag aaaaggtcac tataatattt tggggggtgt tttgggctaa ggtattttta
     3421 gcgtcaactt caatattatt attgcttata ttaattatgg tttttgatat ttatgcgcag
     3481 cattttttag ttatcttagc ttttattcca ctaatagtta gcaatataat ttatccagta
     3541 tggttttttc aaggtataga agaaatgaaa tggataacta tttgtacgtt actggcgaaa
     3601 ctgattattg ttcctacaac atttatattt gtcaataatc ctaatgatat atgggttgcc
     3661 gcttttatcc aaagctgttc tatgatgcta tctggtttaa tctcagtata tattataatg
     3721 agaaagaaat gggtttcaaa aattattttt gattatgttt atataagaaa atgtttaagt
     3781 gatggttggc atctttttat atctacttct gcaataagtt tatatacaac gagtaccaca
     3841 attattctgg gggctattgc tggccctatt gctgtaggtt attataacac ggctaatact
     3901 attagaggag ctatgcaagg gctgctaaat ccaatagctc aagcaattta tcctaggatt
     3961 agttatttga taactgaaga ttaccagagt gctttattct taattaaaag aatgctcaaa
     4021 tatttttgct gcattgcttt attatcttca ctaagtcttt tcatatatgc agcacccatt
     4081 attaaaatag tggctgggac aggttatgat ggtgctgtaa ttgttttgcg ttggatgtca
     4141 tttattcctt tctttgtggc aatgagtaat atatttggtg ttcaaacaat gttaacacat
     4201 gggtataaaa aagagtttag ttttgtactt attattagcg gagtactaaa tattttattg
     4261 atattacctc ttgtttattt cgaaagccag gcaggtgcgg ctatggcatt attattgacg
     4321 gaggtttttg taacggcaat gatgtatatc tttctcagaa agaaaaaaat atttttattt
     4381 aaaaggtgat attaaatgta tgattatctg attgttggtt ccggtttcta tggggctgtt
     4441 tgtgctcatg aattatataa atcaggaaag aaagttcttg ttgttgaaaa aagagaacat
     4501 ttaggcggga atgcctttac tgagaatgtt aaaggcattc aggtgcataa atatggagca
     4561 cacatatttc atactaatga taaattaatt tgggattata taaataaatt ttcagaattc
     4621 aataggttta ctaattcacc aatagcaatt tataaagata aaatatataa cttgccattt
     4681 aatatgaata cattttatca attatgggga gctaaaacgc cgcaggatgc tttagagata
     4741 atcaatgctc aacgtgcaga ctatttacat caaaccccta tgaatttaga agagaaagca
     4801 atatctcttg tagggaaaga tatatatgaa attctaatta aaggctatac agaaaaacaa
     4861 tgggggcgga atgctacgga gctaccgtct ttcattatta atcgcttgcc tgttagattt
     4921 tcattcgaca ataactactt ttcagaccgc tatcaaggaa ttcctatcgg tggatataca
     4981 aaaatatttg aaaaattatt aagcggaatt gatattgaat ttggtgtgga ttatttgcgc
     5041 aataaagtaa aatataatag tcttgctaag aaagtgatat atacaggcgc aattgatgag
     5101 tattatgatt acatatatgg tgaattggag tacagatctt taaagtttga gcatataatt
     5161 ttggacttgg agaactatca gggaaatgca gttgtaaatt atacggatat agctatacct
     5221 tatacacgga ttgttgagca taagcatttt gattttatag agtcttctca tacagtaata
     5281 acaagagaat atccatctgc ttggaaaaaa ggagatgatg cttattatcc gattaatgac
     5341 aaaaaaaaca tggacttatt taaacgttat cgtgcattag ctacggagga aaaaaacatt
     5401 cattttggag ggcgtttggc tgaatataaa tattatgaca tgcaccaggt aattaaagcg
     5461 gcattaaatt tcgttgaaaa ctcttttcgt gaaagttaac tgaatgtctt atttatatct
     5521 attgttttcc ggtatctcct taaccagtaa attgggggtc atagtatttt ggttatcaat
     5581 tcctgtgatt tttttttact gttttggagt atcattgctt tataatagaa aagtgcataa
     5641 aaaatacttc attatagtta tttggagtgt tttttttatt cttatggctg gtttaagcgg
     5701 gtattttctt gatgatttta attcaagggt tatcagtaat gtctatttat ctctagttgg
     5761 tctatttatg gcattagtgt tttataatag gggaaatagc tctaatgata aatcctttct
     5821 tacagcgata aagatcacta tttctataaa tttatgtgct ttctatatac aattcgtttc
     5881 gttctatctc tttcattaca tcattgatta taatttatta agtggagggc tggggggacg
     5941 tttttactgg ggcgatcttt ttcgggcagc aggatttttt gacgagcctg ctgtatattc
     6001 attacatatg gttgctttga cagtaatgct atatatgcag gagagaaaat tttcaattct
     6061 aattattgtt acattgctca gtctattgct atctttttcg tttattgcta tttttcaagc
     6121 aatgattttg agcttgcttt ttattaaaaa taaacgaggc ctgcttgtgc ctctcgtagt
     6181 gtgtttggtt gttgcattac tatttaaaga taatatttat gagcgttatt tacaattcgt
     6241 tagtggaggt gatggtagca acaatactaa gttagatact atttataact tatttcaggg
     6301 agtaaagtgg ctttatggct atggtcttgt aggatattca caatcattcc cactatacta
     6361 tcaaggtttg tatgatttaa cattttgggg agcaaatata agtttatatg ggatagtttt
     6421 aggcttaatt ataaatgtaa ctgtattatt ctttctgctt aaatttacta tgagggactt
     6481 tttattaata aacgttgtcc tgttgaaatt atcaactccc acatatggaa tatatttttg
     6541 ttttcttttt tttctactat ggtataaaca gaatatggta attcgagatg ccaaaaatat
     6601 cagttttaat ggcgataaat aaaaaaacgc cctatttaga taaagcaata aatagcatat
     6661 taaatcaaac atttaaagag tttgagttta taatcatagc caatggatgt gatgatgatt
     6721 tatattattt attaaacgct tactctcgga aggactctcg tattattctt catcgtactg
     6781 aattaggtgg gtttgcattc gctctaaatt atggattgaa tttagccacg acagaatata
     6841 tagcacgcat ggatggagat gatctttgta ctattgaccg tctacaaaaa cagtatgata
     6901 tcattaaaaa taataagtca ctgtctttgc taggaaccgc ttgtaatttt attgggccaa
     6961 atgatgaaga aatattgagt aaaaaattta aaatattgac ggataatgga gcaatcaggc
     7021 gagcgttacc atatagaaat cctataatac acgcttctat tatttgtaga aaagatgtat
     7081 tgctgtctgc tggtggatat agatatggac atatgtctga ggatcatgaa ctttttatta
     7141 gactcgcccg agataaaaat gttatcttta tgaatacgat tgaaccactg tattcatatc
     7201 gtcggcatca tatgcaggtt acagacttat ctaaatctaa ggaaagttat gcacaaatta
     7261 gtgcttttct tttttcagag tttcttttaa cgcataacta taaatatata attggcattt
     7321 tgcgtgtcca tccgtatata aggaaaatgc ataacttttt taaaaggaag tttcataatg
     7381 caaaataaaa tatatcatgt ttgttctggt attcgtccag ggggggggcc tgctggttat
     7441 ttgtataatc tcaatgaagc aagtaaagct actaaacata attttattgt agatgcaagt
     7501 aatgaaagta agataagaag taacacttca tatcgaaaaa taaaaaagct tccaaatttt
     7561 attataaaaa atgcattatg catgaaatat attttagcat taatggggtt cactaaaata
     7621 tctaatgaaa agaatatatt taattttaag tacaatttag gtcattcggt tcctgtagct
     7681 gctagattgt taaaacttaa taaaaataaa acagtaaaaa taggattaat gccacatgga
     7741 cctgtaagtt atactaccga aacactggat gatgttgaat ctaaatatgg taaaatttat
     7801 tttaggggat tttattttag aattatgagt ttacttgaaa ataatatttt caataaggtt
     7861 gattttataa cagttgctgc cagagaaggg atggagtcgt atcaagatat aaatatacaa
     7921 tatgaataca agctttttga agttacttct ggattaccgc cattgatttg taatactaat
     7981 aaaagcgatg caaggaaact attaggtatt ccagatgata aatttattgt ggggttcttc
     8041 ggtaggtata ataggcataa gggatacgat tttttctatg atgaagttat atcttctgca
     8101 aatgaaaaag tgttttttat atcagcaggg attgggccaa taataaaaaa agaggtacat
     8161 aattataaaa actttggatg gcgtagtgat ataaataact taataaccgc ctgcgatata
     8221 atcgtaatac caaatagaca tacttacttc gacttactgc cgcttgaatg tttctcttta
     8281 tctcggcctg tagctgtatc tttaataggt ggcaataaaa aattatgtag ccttactcaa
     8341 gcggcaatac cttttaaatt aaactgtgga gagttgaata atttagtgaa taatttaagt
     8401 aataataaaa ctgattataa aaaattagaa tctttggcta aaaaatcgta cgatgagttg
     8461 ttttctgata aggtatttgt tactaatcat aattctttac ttagttcttt aaatgacttt
     8521 ttttgtaagg aagtggaatg aaatttataa aatgcactct caatgatgtg aaaattattt
     8581 atcccacagt atatcatgac aaaaggggtt tttttttaga agggtttaat gagaaaaaat
     8641 atcaagagtt attaggagta tctggatttg ttcaggacaa tatatcattt tcaatgaaaa
     8701 atgttttgag aggaatgcat tttcaaaaaa gatttccgca aggaaaatta gtgcaggttt
     8761 tgtatggttc aatttatgat gtgattgttg atttaagaaa agattctagt acttatttga
     8821 tgtgggaagg attccatttg gatgcaagag agcatgccca gctttgggtt cctcctggat
     8881 ttgctcatgg ttttttgact ttgtcagact ccgtttattt tacatataaa tgtaccgaat
     8941 attatcgacc attagatgag tgctgtttat tatggagcga tcctgaaatc aatattgatt
     9001 ggcctattcc taatggtgag ctgattgtgt cagaaaagga cgctaatgga ttgtttataa
     9061 aagattttat gtaataaggt agaataaatt aatggctata tatgtttcaa ttatttctca
     9121 tggacatgag gacatgctag caaatactcc acttctaaat gaattatcac agctagctac
     9181 agtgattata aagaataata aaaaaagctc gaaaggtact ctatataatt tgaaagttaa
     9241 tgataatatt attattttgg atgaaaatga ttatgggtta gggtttggtc ataataataa
     9301 ctatgttttt aaatacgcta tagaaaattt agggttaaat ggtaatgatt atttttgtac
     9361 tattaatcct gatattatcg ttgataaact aacagtatct aatgtaattg aggaaatgca
     9421 ctctaggaac tatgagttaa gtacgctatt tttatattta gatgacaata aaaaaataaa
     9481 agatgaatca ttaaggaatt ttccggtttt tattgatttt ttaaaatcat acttcttggg
     9541 gaaaagagat aaagttaagt tagagaaaat tgatataaac gcatatgctg tagattgggc
     9601 cgcgggttca tttttaatat ttaaagcttc cctttttatg aagcttaaag gttttgatca
     9661 gagatatttc atgtattgtg aggatataga tatatgttat agagctaaaa aaatgggtat
     9721 tagattaatc tgcttaacta attatagcgc catccattta gcacaacgtg caagcagaac
     9781 aatttttagt agaaatttcc tgtggcattt aaaaagtata attatttata ctttagcaac
     9841 aaataagaaa gttaaatcga tattgtgatg aatctgtcaa ttatttaact tactgactgt
     9901 taggataagt atattttgtt ttatcataac taacaataag ttaggtgtta gctatttttt
     9961 tgaaaaaatt gaaaactagg actgttttaa gttaacagtt agatactttt gcaaagagga
    10021 gggcgtttaa agaaaattat gatctgtttt cagggacatg tacatactac ataacatctg
    10081 aacatcacta atatagtcaa aatattcata tcgttaattc atctgatggc aaatgggtaa
    10141 attggctcat ttcacgcatc cggacgtgct tcactatgac caggtgctat cgttttaaat
    10201 aattatggct gtgcttatat tggagctcgg tattgctgag ctgtatcatc actctgattt
    10261 aagcttaatg ttgcatagct actttttaca cggatactta aattggtgga tgtgactatt
    10321 gcctcgcgta aagcaatatc aactttgaca atcaatgatt tattgtgtaa agaaagaaaa
    10381 attactattg agcatatctt ggctcagcgt ccagtagaat atcttgtgtt ttatcaggat
    10441 aaagttaata ttcatctaaa tttaaaattg gaatagactg gatgccaaag aggaagcgga
    10501 aatacatagc cataactaat atagaatcag agaccttatc taaactaatg caatattagt
    10561 atatagatca atgccgttag taataatttc tagcttattt atcagtttca tgttaatgct
    10621 taatagttac gtcatttata aaatttggaa agtaggctag taatatatag ttattctatc
    10681 tatattttat tacacatatt aatcactcta ttctattacc attttatctt atttagagat
    10741 tgttttttgc taagttaata cttagcgaag agattcagct ccgtatagtg aaatgaaatt
    10801 tatattagtg atcttataac ttaattggtg ttactagatt ctacacctaa ttcaattaag
    10861 cataatctga ttattgatta ttgattattg attattgatt attgattatt gattattgat
    10921 tattgattat tgattattgt taaaactatt aaggtaatta aatgacaatt ttagtgactg
    10981 gtggcgcagg gtatataggt tcacataccg tgctttgtct tcttgaaaat aactataatg
    11041 ttgttgttct tgataacttt attaactcgt cagaacaatc tctaaagcgg gtaagttcac
    11101 ttgctaaccg tgacgttaaa atctataatg gtgatattcg taaccggatc ttattgaata
    11161 atattttttc ggaacaaaat attactcatg ttattcattt tgcaggactc aaatctgtta
    11221 gcgaatcagt taacgaacca ataaattatt atgataataa tttaaacgga agccttatat
    11281 tggttgacat gatgttaaaa tacaaaattt ataatttact atttagctca tccgccacag
    11341 tctatggaac tcccacttcc gtgccagtaa atgagaaatc acatgtgggt ggcacaacta
    11401 acccttatgg aacttccaaa ttaatgattg aaaaagtatt aggtgacatc gcaactgttg
    11461 aaccaaaatt tagagtaaca acgttacgat attttaatcc tgttggagcc cattgttcag
    11521 gattaattgg cgaggatcct aatggggtgc ctaataattt aatgccatat atttgccagg
    11581 ttgcaattgg aaaacaaaaa caattactta tttatgggaa tgattatcca acaaaagacg
    11641 gcaccggcat acgagatttc gttcatgtta tggatcttgc tgaggggcat cttgcagcct
    11701 taagacacag tgatgaagga cctaattata aagtttataa tttaggtact ggtatagggt
    11761 atagtgtgct tgagatttta gaaacttttc aaaggattac ttctaaatat gtgcctcgcg
    11821 tttttgttga tagacgccat ggtgatatag ctgaatgttg gtcagatcct tctagagcaa
    11881 ataaagaact ttgctggaaa gcaaaacgag gtcttgaaga gatggttcgt gatgcttgga
    11941 attggcaaca acgtaatccc aatggctata caaaatctaa taataattaa ttataccttt
    12001 aagttataac gcaagatggt ttgtcacttt gataaaattt tgccttgcat agcacaattt
    12061 gattggtagt aaaatattaa aaatcatcag tcagatctgg cgatatccat cataaaacta
    12121 attattttta tcaggatgat gtctgaatca gacaatgaac tacaagatat gtgtatggtc
    12181 atactgaaag attagctatt atattgctat tgtatagcag gggattatta atgacaatgt
    12241 tgttgtaatt atttatcaga tagggaatta attgctttag gtgaagatct aattccttta
    12301 ccaacggagc gaggttgctg atagtgtaat aaaaatattt aagaattatt atatttgatt
    12361 gcaataatac acaaagtaat tttttcttgc aatttttgta tttttctttc atataaaatt
    12421 gcgaaagttg tggttcttta tcttataagc aataagtatg tatacagctc tgtccgaata
    12481 ttgaattagc tataggctta tatttattaa ataaatgtta atggagtgag ttatctgcct
    12541 tattatatgt atagataaaa tttatattta tttatcaact tggtaaagta tttgtaagtt
    12601 aagtctcgat gggctaaaaa attcacattc acattcacat tcacattcac attcacattc
    12661 acattcacat tcaactagca taaaagccaa aaattttgag tcaattataa atgataaaaa
    12721 cctaactgga gttagaatta ttatcaactg atatattcat cataaataag ttaaacattt
    12781 ttatcatttt ctgaaaattt gatacttctt gaaaaaactc ttttaaggct gacgtgctat
    12841 tgcaaaagga tagcgatact tgttatttat taatacgttg cagtcaagta agagcaacat
    12901 attgcaatct tggcgtagtg ctttttaggg ggagatagca gcatcaatca attgccttgc
    12961 aaggactagg aaaaatatag aactattccg tgcagctatt tagtaaggtt attaaagtta
    13021 acgtagctat tattatctac gttatattct acagatccac acctgacagg agtatgtaat
    13081 ggccaagcaa cagatcggcg tcgtcggtat ggcagtg
//
