LOCUS       PWQRRMP                 6915 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Plasmid pWQ799 RNAII and RNAI genes, complete sequence; RNAI
            modulator protein (Rom), mobilization proteins (mbeC, mbeA, mbeB,
            and mbeD), N-acetylmannosamine transferase (wbbE), wbbF, and
            UDP-N-acetylglucosamine 2-epimerase (wecB) genes, complete cds.
ACCESSION   L39794
VERSION     L39794.1
KEYWORDS    .
SOURCE      Plasmid pWQ799
  ORGANISM  Plasmid pWQ799
            other sequences; plasmids.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Keenleyside,W.J. and Whitfield,C.
  TITLE     Lateral transfer of rfb genes: a mobilizable ColE1-type plasmid
            carries the rfbO:54 (O:54 antigen biosynthesis) gene cluster from
            Salmonella enterica serovar Borreze
  JOURNAL   J. Bacteriol. 177 (18), 5247-5253 (1995)
   PUBMED   7545154
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Keenleyside,W.J. and Whitfield,C.
  TITLE     A novel pathway for O-polysaccharide biosynthesis in Salmonella
            enterica serovar Borreze
  JOURNAL   J. Biol. Chem. 271 (45), 28581-28592 (1996)
   PUBMED   8910488
REFERENCE   3  (bases 1 to 6915)
  AUTHORS   Reeves,P.R., Hobbs,M., Valvano,M.A., Skurnik,M., Whitfield,C.,
            Coplin,D., Kido,N., Klena,J., Maskell,D., Raetz,C.R. and Rick,P.D.
  TITLE     Bacterial polysaccharide synthesis and gene nomenclature
  JOURNAL   Trends Microbiol. 4 (12), 495-503 (1996)
   PUBMED   9004408
REFERENCE   4  (bases 1 to 6915)
  AUTHORS   Keenleyside,W.J. and Whitfield,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-1995) Microbiology, University of Guelph, Guelph,
            ON N1G 2W1, Canada
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Plasmid pWQ799"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:38174"
                     /plasmid="pWQ799"
                     /note="isolated from Salmonella enterica serovar Borreze;
                     polysaccharide genes named in accordance with the
                     Bacterial Polysaccharide Gene Nomenclature scheme"
     repeat_region   35..42
                     /rpt_family="repeat A"
                     /rpt_type=inverted
     gene            91..664
                     /gene="RNAII"
     regulatory      91..96
                     /regulatory_class=minus_35_signal
                     /gene="RNAII"
                     /note="promoter region"
     regulatory      115..119
                     /regulatory_class=minus_10_signal
                     /gene="RNAII"
                     /note="promoter region"
     misc_RNA        127..>664
                     /gene="RNAII"
                     /product="regulatory RNA"
                     /function="regulation of replication of ColE1-related
                     plasmids"
     gene            complement(132..274)
                     /gene="RNAI"
     misc_RNA        complement(132..238)
                     /gene="RNAI"
                     /product="regulatory RNA"
                     /function="regulation of replication of ColE1-related
                     plasmids"
     repeat_region   complement(133..146)
                     /gene="RNAI"
                     /rpt_family="repeat B"
                     /rpt_type=inverted
     repeat_region   151..164
                     /gene="RNAII"
                     /rpt_family="repeat B"
                     /rpt_type=inverted
     repeat_region   165..176
                     /gene="RNAII"
                     /rpt_family="repeat C"
                     /rpt_type=inverted
     repeat_region   187..198
                     /gene="RNAII"
                     /rpt_family="repeat C"
                     /rpt_type=inverted
     repeat_region   200..211
                     /gene="RNAII"
                     /rpt_family="repeat D"
                     /rpt_type=inverted
     repeat_region   219..231
                     /gene="RNAII"
                     /rpt_family="repeat D"
                     /rpt_type=inverted
     regulatory      complement(245..250)
                     /regulatory_class=minus_10_signal
                     /gene="RNAI"
                     /note="promoter region"
     regulatory      complement(269..274)
                     /regulatory_class=minus_35_signal
                     /gene="RNAI"
                     /note="promoter region"
     rep_origin      664..666
                     /standard_name="oriV"
     misc_feature    921..922
                     /standard_name="nic site, relaxation site"
                     /function="site in ColE-related plasmids where a single
                     stranded cut is made to initiate plasmid transfer during
                     conjugation"
     gene            complement(1068..1259)
                     /gene="Rom"
     CDS             complement(1068..1259)
                     /gene="Rom"
                     /codon_start=1
                     /transl_table=11
                     /product="RNAI modulator protein"
                     /protein_id="AAC98396.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDCENL"
     gene            1324..1647
                     /gene="mbeC"
     CDS             1324..1647
                     /gene="mbeC"
                     /standard_name="MbeC mobilization protein"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="AAC98397.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHG
                     ARDDS"
     gene            1625..3190
                     /gene="mbeA"
     CDS             1625..3190
                     /gene="mbeA"
                     /standard_name="MbeA mobilization protein"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="AAC98398.1"
                     /translation="MERGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGDPEEV
                     RELIDATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQ
                     DKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRI
                     LTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISI
                     ADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQQARRICKQGCDL
                     KRDENQRRYSPVHSLDREIAGKTQGRGERGDDAAQEGRVKAGREYGNDVTGDSLSPVY
                     REWRDALVSWRADTGEPGRNQDTGRNIAETEREDMGRGVCAGREKEISGPSVREIGNG
                     DSLSGEGLGTTDGVTQSDGAGKTFAERIRATATGLYAAAERMGERLRGIAENVFAYAA
                     GQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQEREHELSLERERQPE
                     IQERTLDGPSLGW"
     gene            2325..2843
                     /gene="mbeB"
     CDS             2325..2843
                     /gene="mbeB"
                     /standard_name="MbeB mobilization protein"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="AAC98399.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFSKLGESVSKAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVI
                     PYQGKDWVQLTE"
     gene            2850..3083
                     /gene="mbeD"
     CDS             2850..3083
                     /gene="mbeD"
                     /standard_name="MbeD mobilization protein"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="AAC98400.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
     misc_feature    3187..>3429
                     /standard_name="par region, partition region"
                     /function="region in ColE1-related plasmids where a
                     site-specific rcombination event occurs to resolve plasmid
                     multimers"
     gene            3252..3363
                     /gene="Rcd"
     regulatory      3252..3258
                     /regulatory_class=minus_35_signal
                     /gene="Rcd"
                     /note="promoter region of the partition region RNA"
     regulatory      3273..3278
                     /regulatory_class=minus_10_signal
                     /gene="Rcd"
                     /note="promoter region of the partition region RNA"
     misc_RNA        3286..3363
                     /gene="Rcd"
                     /product="partition region RNA"
                     /note="putative"
     repeat_region   3347..3350
                     /gene="Rcd"
                     /rpt_family="repeat E"
                     /rpt_type=inverted
     regulatory      3347..3359
                     /regulatory_class=terminator
                     /gene="Rcd"
                     /note="stem-loop structure; putative"
     repeat_region   3356..3359
                     /gene="Rcd"
                     /rpt_family="repeat E"
                     /rpt_type=inverted
     repeat_region   3373..3378
                     /rpt_family="repeat A"
                     /rpt_type=inverted
     gene            3547..4371
                     /gene="wbbE"
                     /note="previously referred to as rfbA"
     CDS             3547..4371
                     /gene="wbbE"
                     /standard_name="ManNAc transferase"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAC98401.1"
                     /translation="MGHQFTVCMVVTRNDAAEHFNVALLSLVCQSMKPSEVLIVDNGG
                     LTDRHYEIIANIGDELSIRLLRIAHLSSVGQARNIALHAARYQVIAVADPDDINEPDR
                     FIKLIPELNDSVRMVGAWVREFSREKNDRGIVRRVPCEFVDIIRYSRFRSPVNNPTIC
                     YFKNDALSVGGYNTSLHFGEDYDLVTRFIRNGKIIRNIPIVMVNFRIGDAKKLYKKRG
                     GLFLLKQEINLHRRFLENRYISLLDFFMIVTIKILVRLLPCWLFNVFYFKVLRKHE"
     gene            4364..5743
                     /gene="wbbF"
                     /note="previously referred to as rfbB"
     CDS             4364..5743
                     /gene="wbbF"
                     /note="WbbF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAC98402.1"
                     /translation="MNDYIIDIVEYVLYVLVTAMTWYLFALSSYSIFLSVFGFAKNKK
                     DYPDCPPEARFLILVAAHNEEAVIGSTLINLKNIQYDKKLFDIVVVNDNSTDRTGLIC
                     DSHEVKHVDTIEGEFEREGVGKPAGIQYALRKLGFETVKENYDLVMVLDADNFVDANI
                     LTELNSQWISKDKPEAIQAYLDCKNSTSLLSFGYCTSYWMMNRFFQLSKYRLGLPNAI
                     GGTGFVVSSNFLINTGGFCFKSLTEDIELEIEIVRKRGRVLWNHNVRVYDEKPDNLRI
                     SLKQRYRWSKGHWYVAFTNFFNLFKLTFVERKWKYVDQLLYLFSMGRAFEVCIIFINI
                     FLLSLLKENYHPEIGNISTAIKDLTVTNMSFADSVSAQFSSINWIRVITNINIVTLIS
                     ICYGMLILPIYGAWMDKGIFLNPFRVFFSGLYFGLSFVFVQFLALFRWKKQHKWVVTP
                     HNKIKEEHK"
     gene            5740..6876
                     /gene="wecB"
                     /note="previously referred to as rfbC"
     CDS             5740..6876
                     /gene="wecB"
                     /EC_number="5.1.3.14"
                     /standard_name="UDP-GlcNAc-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="AAC98403.1"
                     /translation="MSKVLFVFGTRPEAIKMAPLVIEFKNNPAIEVKVCVTGQHREML
                     DQVLDFFEIEPDYDLNIMKQKQSLGSITCSILTRLDEILASFMPAHIFVHGDTTTTFA
                     ASLAAFYQNIKVWHIEAGLRTWNMNSPFPEEGNRQLTSKLAFFHAAPTLQAKDNLLRE
                     SVKEKNIIVTGNTVIDALLIGIKKITGSTGDVREIISLKNKLNLDKKIILVTLHRREN
                     QGELLRTICDDIKQLALEHDDIEIVFPVHMSPRIREVVNEKLSGVVNIKLVEPLAYPG
                     FIWLMNNAHFILSDSGGVQEEAPSLQKPVLVARDTTERPEVIENGAAMLVDPRIPNNI
                     YSSCKKLLSDERLYEKMSQAGNPFGDGKASKKILDYFVSLEDIK"
BASE COUNT     1859 a   1367 c   1816 g   1873 t
ORIGIN
        1 ttctggtcgc agaaaaatct aggtatccgg cacagaaccc cattggcgga ctggtgtcaa
       61 agttgttcag tagaaattaa aaggatcttc ttgagatctt tttttcctac gggtaatctt
      121 tcgctttgta aacgaaaaaa cccgcctgat ggcgggttga ttcgtccagt tagaagtctt
      181 cgacctcttc taactggagt ggtagctggg tatacgagac cgcagatacc aaaactgttc
      241 ttccagtgta gccgaaatta ggcgaaagct tcaagacctc tctgaatatc tctctcgtat
      301 aattccagtt gccagtggct gctgccagtg gcgctttgtc gtgtctttcc ggattggact
      361 caagtcgata gttaccggaa caggcgcagc agtcggactg aacggggggt tcgtgcatac
      421 agtccatcct ggagcgaact gccttcgcgg aactgagtgt caggcgtgga atgagaaacc
      481 gcggccataa cagcggaatg acaccggtaa accgaaaggc gggaatgcgg gggagcacga
      541 gggagccacc agggggaaac gcctggtatc tttaagccgc atcgggtttc gccaccactg
      601 atttgagcac cggaaatcat ccgatttttg tgaagctcat caggggggcg gagcctatgg
      661 aaaaacggca acgccgcggc cttttttcct cgtgttgtgc gggttctttc gcgtcgttct
      721 cctcccctca gctattagga attttcccga tattgtgccc gtactgctcg ccgcagtcga
      781 acgtccgagc ggagcgagtc agtgagcgag gaggcgtcat ttcatccatc cttccatttt
      841 cttctgacgc gcctacgcct cattttactc ctgcaacaga gtgcactttt ttcattaccg
      901 cacccatgcc aatataataa gccagtatac actccgctat cgctacgtga ctgggtcatg
      961 gctgcgcccc gacacccgct aaaacccgct gacgcgccct gacgggcttg tctgctcccg
     1021 gtatccgctc acaggcaagc tgtgaccgtc tccgggagct gtatgtgtca gaggttttca
     1081 cagtcatccc cgaagcgtgc gaggcagctg cggtaaagct cgtcggcgtg gtcgtgtagt
     1141 gattcacaaa tatcggcctg ttcatctgcg tccagttcgt tgagcttctc cagcagcgtt
     1201 aatgtctggc ttcttataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     1261 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgcgag
     1321 aggatgctca cgatacgggt tactgatgat gaacatgcac ggttacttga acgctgtgag
     1381 ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gggagccggt tgcccgttcc
     1441 gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgccat cgggaataac
     1501 ctgaatcaga cggcgaggaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag
     1561 gtggtggccg cactgatggc catcgagaga gagttgcgca gcttacgaca ggtcgtcagg
     1621 gaacatggag cgcgggatga tagttaagtt tcatgccagg ggaaaaggtg gtggcagtgg
     1681 tccggttgat tacctgctgg gaagggagcg taaccgggaa ggggcaactg tgcttcaggg
     1741 tgatccggaa gaagttcggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc
     1801 aggtgttctg tcgttcgcgg agaaggagct gccgccggga gggcgtgaaa aagtgatggc
     1861 gagctttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt
     1921 ggagcaccag gacaagggac ggcttgagct taattttgtc attccgaaca tggagctaca
     1981 gagcggaaaa cgcctccagc cgtactacga ccgcgcagac aggcctagaa ttgatgcctg
     2041 gcagacgctg gtgaatcacc attacgggct gcatgacccc aacgccccgg agaaccgccg
     2101 gatactgaca ctccctgata acctcccgga aacgaaacag gcgcttgctg agagcgtcac
     2161 acgtgggata gatgctcttt atcatgtcgg ggagataaaa ggccgtcagg atgtgattca
     2221 ggcgctcaca gaggcggggc ttgaagtggt cagggtgacg cgaagcagta tcagcattgc
     2281 agacccgaac ggcgggaaga atatcaggct gaaaggagca ttttatgagc aatcttttac
     2341 agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc
     2401 tgaacgacga gttcagcaag ctcggcgaat ctgtaagcaa ggctgtgacc tcaaacgaga
     2461 cgaaaatcag agacgctata gccctgttca cagcctcgac agagaaatcg ctggaaaaac
     2521 acagggaagg ggtgaaagag gcgatgatgc agcacaggaa ggacgtgtta aagctggcag
     2581 ggaatacggg aatgatgtta ctggggatag tctttctcct gtttaccgcg agtggcggga
     2641 cgctctggta tcttggcggg cggatacagg cgaacctgga agaaatcagg atacaggaag
     2701 aaacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag gacgggagaa
     2761 ggaaatttct ggtccttccg tacgggaaat cggcaacggt gattccctat caggggaagg
     2821 actgggtaca actgacggag tgacacagag tgacggagct ggaaagacat ttgctgaacg
     2881 cattagagca actgcaacag gactatatgc agcggctgaa cgaatgggag agcgccttcg
     2941 tggaattgca gaaaatgttt tcgcttacgc agcgggacaa cgcgatgctg aacgagcagg
     3001 tcatgcagtt gagtcagcag gtgcagcact tgagcgagcg gacagaacgc ttgagccagt
     3061 tatacagcga gaacaggaga taagggacga acggctgata caggaacgcg aacatgagtt
     3121 atccctggag cgtgagcgcc agccggaaat acaggaacgc acactggatg gtccttcact
     3181 gggatggtga aactatgaaa aatggcaggt taagtggatt aagacgggct aatgtggtct
     3241 gtaccatctg gttgcatagg tattcatgcg gttaaaattt atcaggcgcg atcgcggcag
     3301 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     3361 tttagcggtg cgcccaagga atgttatgtt taatatgaac atcggacaga taaaaaaacc
     3421 gtctatcctg ggccatcgtt gaattggagc tgtagttgac atatcttagt attgtgcttt
     3481 taggtgtaaa tcatttttac ctatctgcat ggcgatgctg tataggaaat taggaaatta
     3541 gggaatatgg ggcatcagtt tacggtttgc atggtggtta cacggaacga tgcagcagaa
     3601 catttcaatg ttgcgctgtt atctcttgtc tgtcagtcca tgaaaccatc ggaggtattg
     3661 attgttgata atggtggatt aacagatcga cattatgaaa taattgctaa tataggtgat
     3721 gaactaagca ttcgcttgtt gagaatagca catctaagct ctgtggggca ggcgagaaac
     3781 atcgcactgc atgctgcaag gtaccaagtt attgctgttg cagatccaga tgatattaat
     3841 gagcctgacc gttttattaa gcttattcct gagctaaacg atagcgtaag aatggtgggg
     3901 gcatgggtgc gagaatttag tcgtgagaaa aacgatagag gcattgtgcg tagagttcca
     3961 tgtgagtttg tggatattat tcgctattca cgatttcgat cacctgttaa taacccaact
     4021 atctgttact ttaaaaatga tgcactgagt gtaggggggt ataacacgtc tttacatttt
     4081 ggagaggact atgatttagt gacgagattt atccggaatg ggaaaattat acgaaacatt
     4141 cccattgtta tggtgaactt ccggattggt gatgctaaaa agctttataa aaaaagaggt
     4201 gggctctttt tactcaagca ggaaataaat ctgcatcgaa gatttctgga aaatagatat
     4261 atttcgctat tggatttctt tatgattgta actataaaga ttctggttag gttattacct
     4321 tgctggcttt ttaatgtgtt ttattttaag gtgttaagaa agcatgaatg attatataat
     4381 tgacatagtg gaatatgttt tatatgtgct tgtaactgcc atgacatggt atttgtttgc
     4441 cctgtcttcc tattccattt ttttgagtgt atttgggttt gctaaaaata aaaaagacta
     4501 tcctgactgt cctcctgaag cccgctttct aattcttgtg gctgcgcata atgaagaggc
     4561 tgtgattggc tcaacactta ttaatcttaa gaatattcaa tatgataaaa aactctttga
     4621 tatagtagtg gttaatgata attccactga tcggacagga cttatctgtg atagtcatga
     4681 agtaaagcat gtggatacta tagaaggcga gtttgaacgt gaaggtgtgg gcaaaccagc
     4741 tggtattcag tacgctctgc gcaagcttgg atttgaaact gttaaagaaa attatgattt
     4801 ggtcatggtg ttggatgccg acaattttgt tgatgcgaat atccttactg agcttaattc
     4861 tcaatggata tcaaaggaca agccggaagc tatacaggcg tatctggatt gtaaaaactc
     4921 aacatctctt ctctcttttg gctactgtac atcatactgg atgatgaatc gatttttcca
     4981 attatcaaaa tatcgcttag gtctacctaa tgcaattggc ggaacaggat ttgtagtgtc
     5041 cagtaacttt ctgataaata ctggaggatt ttgttttaaa tctctgaccg aggacattga
     5101 actggaaatt gaaattgtgc gcaagcgtgg tcgtgtacta tggaatcaca atgtacgagt
     5161 ctatgatgaa aagccagaca atctaagaat aagcctcaaa caacgctatc gctggtcaaa
     5221 gggacactgg tatgtggctt ttactaactt ttttaacttg ttcaagttga cctttgttga
     5281 gcgtaagtgg aaatatgtag atcaattgtt atatctgttc tctatgggcc gtgctttcga
     5341 ggtttgtatt attttcatca atatctttct attaagtctt cttaaagaaa attaccatcc
     5401 agagattgga aatatttcta cggcgataaa agatcttact gtgaccaata tgagttttgc
     5461 cgacagcgtg agtgcgcagt ttagctcaat aaattggatt agagttatta ctaatattaa
     5521 tatcgtcaca ttaatatcca tctgttatgg tatgctgatt ttacctattt atggtgcatg
     5581 gatggataaa ggtattttcc ttaatccatt cagggtattt ttttccggtc tttattttgg
     5641 attgtcgttt gtgtttgttc agtttttggc tctttttcgt tggaaaaaac agcataaatg
     5701 ggtagttacg ccgcataata aaataaagga agagcacaaa tgagcaaggt tttattcgtt
     5761 tttggtacgc gtcctgaggc cattaagatg gctccacttg tcatagagtt taagaataat
     5821 ccagctatcg aggtgaaagt ctgcgttacg ggacagcatc gagaaatgct tgatcaggtg
     5881 ctggattttt ttgaaattga accggactac gatctgaata tcatgaagca gaaacagagt
     5941 cttggttcca ttacatgctc tattttaact cgcctagatg agattctggc gtcgttcatg
     6001 cctgctcata tctttgtcca tggcgatacc actactacat ttgcagcctc tttggctgcc
     6061 ttctaccaaa acattaaggt atggcatatt gaggctggtt tgagaacttg gaatatgaat
     6121 tcacctttcc ctgaagaggg aaatcgacaa ctcacaagta agctcgcttt ttttcatgct
     6181 gcacctactc ttcaagctaa agataatctc cttcgtgaaa gcgtgaagga aaaaaatatc
     6241 attgtcactg gtaatacggt tattgatgca ctgttaatag gtatcaaaaa aataacaggt
     6301 tccactggtg atgtccgaga aattataagt ttgaaaaata agctaaatct ggataagaaa
     6361 attatcttgg ttacattaca ccgccgcgag aatcaggggg agttgctgcg aactatctgt
     6421 gatgatatta aacagttggc actggaacat gatgatattg aaattgtttt tcctgttcat
     6481 atgagtccac gtatccggga agtggttaat gagaaactgt caggagtggt caatatcaaa
     6541 ctagttgagc cattggcata tccgggattt atatggctta tgaacaatgc acatttcatt
     6601 ctgagtgata gtggtggtgt acaagaagaa gctcccagct tacaaaaacc tgtgctggta
     6661 gccagagaca ctacagaaag gccggaagtc attgagaatg gcgcagctat gctggttgat
     6721 ccacgtattc ccaataacat ctattctagt tgcaaaaaac tcctttcaga tgagaggctt
     6781 tatgaaaaaa tgagtcaggc ggggaatcca ttcggcgatg gaaaagcgag caaaaaaatc
     6841 ctcgattatt ttgtatcctt ggaggatatt aagtgatcgt cactcaactg gtcgttttgt
     6901 gacagcgcac gccgt
//
