LOCUS       JX975337               13727 bp    DNA     linear   BCT 27-AUG-2013
DEFINITION  Salmonella enterica strain G1604 O antigen gene cluster, complete
            sequence.
ACCESSION   JX975337
VERSION     JX975337.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Reeves,P.R. and Wang,L.
  TITLE     Structural diversity in Salmonella O antigens and its genetic basis
  JOURNAL   FEMS Microbiol. Rev. (2013) In press
   PUBMED   23848592
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Reeves,P.R. and
            Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1604"
                     /db_xref="taxon:28901"
                     /note="serogroup: O62"
     misc_feature    1..13727
                     /note="O antigen gene cluster"
     gene            1139..2224
                     /gene="rmlB"
     CDS             1139..2224
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFW04775.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIKDTQDTVVNVDKLTYAGNLESLT
                     EVSESHRYHFEHADICDKAAMERIFVTHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARSYWNALDVEKKAAFRFHHISTDEVYGDLPHPDEVSAGSVLPLFTEKTA
                     YAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTQGVVGETYNIGGHNEKKNLDVVQTICDL
                     LDEIVPKEGAYRDQLIYVTDRPGHDRRYAIDAAKISDELGWKPQETFESGIRKTVEWY
                     LNNTQWVENVKSGTYKTWIEQNYGERQ"
     gene            2224..3123
                     /gene="rmlD"
     CDS             2224..3123
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AFW04776.1"
                     /translation="MNILLFGKNGQVGWELQRSLAPLGKIIALDVHSKDYCGDFSNPE
                     GVAETIRRIRPDVIVNAAAHTAVDKAESEVEFATLLNARSVEAIAKAASETGAWVVHY
                     STDYVFPGTGETPWCEDDATAPLNVYGETKLTGEKALQDNCARHLIFRTSWVYAGKGN
                     NFAKTMLRFAKEREEMSVINDQYGAPTGAELLADCTAHAIRVALKQPEVAGLYHLVAG
                     GATTWYEYAALVFDEARKAGIGLAINKLNAVPTSAYPTPARRPNNSRLNTDKFQKKFD
                     LVLPEWEIGVRRMLAELFTTTTV"
     gene            3177..4055
                     /gene="rmlA"
     CDS             3177..4055
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFW04777.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSML
                     MLAGIRDILIISTPQDTPRFEQLLGEGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGN
                     DDCALVLGDNIFYGHDLPRLMETAVNKESGATVFAYHVNDPERYGVVEFDKDGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKKLAIPL
                     SKNAYGQYLIRMVKGY"
     gene            4057..4602
                     /gene="rmlC"
     CDS             4057..4602
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /protein_id="AFW04778.1"
                     /translation="MKVIKTDIPDVLIFEPKVFGDKRGFFMESFNQRVFDEALGHKVE
                     FVQDNHSKSSKGVLRGLHYQLPPYAQGKLVRCVVGEVFDVAVDIRKDSETFGKWVGVN
                     LSADNKKQLWIPEGFAHGFYVLSETAELLYKTTNYYNLLSDRGIIWNDRSININWPIS
                     GDILLSEKDMNHGTLTETSNI"
     gene            4633..5907
                     /gene="gnaA"
     CDS             4633..5907
                     /gene="gnaA"
                     /note="GnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="AFW04779.1"
                     /translation="MKLENINIGIVGLGYVGLPLAVEFGKKFETIGFDIKSTRVEELK
                     NNIDTTLECSSDELQSAKLLQFTNNIEDIKKCNVYIVTVPTPIDKFKRPDLSPLVKAS
                     KLLGSILNKGDVVIYESTVYPGATEEDCVPVLEQQSGMVFNKDFFVGYSPERINPGDK
                     EHRVTSIRKVTSGSTTEIADFVDSIYASIIKAGTYKASSIKVAEAAKVIENTQRDLNI
                     ALINELAIIFNKLNIDTEEVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQSIG
                     YNPEVILSGRRINDAMGEYVASQLVKKMIKTKIQIDYADVLVMGFAFKENCPDLRNTK
                     VIDIISSLKDYNINADVYDPWISPDEAVREYGVNIHNNIPEKKYDAILFAVAHDEFKK
                     MTMGEILSLTKSNYFIYDLKYIIDSDLVIERL"
     gene            5937..6959
                     /gene="gnaB"
     CDS             5937..6959
                     /gene="gnaB"
                     /note="GnaB"
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="AFW04780.1"
                     /translation="MNYDELQDYLLNNQRTWLITGVAGFIGSNLLEKLLKLNQNVIGL
                     DNFSTGFQSNLNEVKDNVSDGSWRRFKFIEGDIRDLDVCKKSVSGVDYVLHQGALGSV
                     PRSIENPILTNASNVSGFLNILDCARRENVKSFTYAASSSTYGDHTGLPKVEDIIGNP
                     LSPYAVTKYVNELYAGVYALNYNFKSIGLRYFNVFGRRQNPNGAYAAVIPKWILSISN
                     GDDLYINGDGSTSRDFCYIDNVVQANLLAALANDNIKNNVFNIAYGQQTSLNKLFEYI
                     AGALDNEGIEYDKQPVFKDFRKGDVRHSLANIDKAKKFLGYEPCYDINEGIKLAIKWY
                     INNLSK"
     gene            7073..8278
                     /gene="wzx"
     CDS             7073..8278
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFW04781.1"
                     /translation="MYLKILIDNFSLVIQYFFGGIAVFYVTPLIVKSVGIHSYGNLAI
                     MFAIVTYISLIIQYSFNLIGPKLIAEGNCKKTFNIIFSAKLVLFILSLAIFLLYFFCF
                     QSKYDNVFLLFFILPLSWVLNNSWFLQSKGYFILSSICSIIGSLVTFVIAYFFINATV
                     GLLVPILCLILSSFINGSLTFFFALKKIGYVKIINPLPILKTGFPLFASQIVSSVYTM
                     SGVIIITHFYGVTSAGTYAVVERFMNLLISLGILTHVAAYPKLARLFNKDRLEYRKTL
                     LFVVALYTFFSCCVALGVFNFHQDIVNYMFGSENGNANELIYSACIYIFVSIYGPVVT
                     GYLALKMEGKLIIIINIAIALLSLLAGVGMLKIMGNSGWLIGLSLAQLLYIIIFFKIF
                     IWGRKECVV"
     gene            8266..10113
                     /gene="wbpS"
     CDS             8266..10113
                     /gene="wbpS"
                     /note="WbpS"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="AFW04782.1"
                     /translation="MCGLVGYFSRSNCDANIIHNMLHKIRHRGPDSFGVWESLECNIH
                     FGHVRLSIVELSSAGHQPMSTSCGRFTIIFNGEIYNHLDIRKELGNEIKWTGTSDTET
                     LLKSISTWGVSGTLNKAVGMFSFALWDSVERSLYLARDRIGEKPLYYGWCNGSFLFGS
                     ELKALKSHPDFDAEIDWEAVNGYLHNNYISSPLTIYSKLKQLRPGHYIKMSHDDLLSG
                     DTPVLYKYWSLSFPVSENKHRYVDSESKLEALLSESVSLQSIADVKVGAFLSGGIDST
                     TIVAMLKKSGRDVSTFSIGMPNKQYDESRHAEDIAKYIGTQHYTHMITPQEALEVIDK
                     IPAIWDEPFADSSQIPTYLVSKFAKEHVTVALSGDGGDELFMGYNQYPLLRKIWDTRY
                     LSNLHLDTMAKIITKMGFKHSSIIARKILNLSQGWRCKTPFLLNDFWMDKYRNTEFPL
                     LQPVPYKRNLDLNYLDGISAITQHDLNYYLCDDILTKVDRASMAVSLETRAPFLDHRI
                     VEFAFGLPTSFKMDKYNQKKILKSVLYKHVDSKLLERPKQGFSLPMNQWLRAELKGWA
                     RDRLDSLPEGVFDKIVVDNLWKEHIDDVKDNSERIWALSNLANFLESHE"
     gene            10106..10855
                     /gene="wcnX"
     CDS             10106..10855
                     /gene="wcnX"
                     /note="WcnX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04783.1"
                     /translation="MNKIAIYTCITGGYDFIKAPLKINDDIDYICFSDQKIPASYPWK
                     IINISDLEISEGFDEKTINRAIKICPQKFGLLEQYELTIYIDGSIEIIDDLSLLIDFV
                     AKQDDVIFMYDHFLRNCLYDEAEECLLIGYDWYWNIRKQVRNYRQKGFPVSYGLFECG
                     IIIRKKSQDLNIIMWKWLEEYIKGVKRDQLSLTYILWESNYHLCSLGESDARYKNRHF
                     KLHKHSIKSGYYFRKFRSKLNKVLIRIFGGL"
     gene            10857..11723
                     /gene="wcnY"
     CDS             10857..11723
                     /gene="wcnY"
                     /note="WcnY"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04784.1"
                     /translation="MYEKVCGVVIVFYHPNDENINSAKKLSELYKVTIVDNSEKDINY
                     FIPKAHIIKLNRNVGIAAALNIGINFFIENDYKYVILLDQDSEPDTLLLSSLINYSER
                     CTDDVCLVAPSYYDRAIDKNAEFILCTDKGIIRQPAIGKNAIEASYVITSGSVLRLSS
                     VSNIGLMNDDLFIDFVDIEWCLRANSLGYKILGLPWLKMSHEIGDKPINIFNKKYVNH
                     SPIRHYYYFRNIFLLMRMSHIHPQWKKWELIKLLPRFFVYACFTKNNMKHISYMLTGI
                     YDGLLGRVGKKK"
     gene            11720..12742
                     /gene="wzy"
     CDS             11720..12742
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFW04785.1"
                     /translation="MNGVAKPIIIFLFACCTLVTFLSCNLNLFPDYYSYLNWYELSAS
                     TNMNNDWVFFKDPGFYLLSVISNNFGFGVIGVIFSLFIISLSSKIFFCIKLLNRETLF
                     WVLLLYLSRLFIVHDLVQYRAGASIGLSALFLYFYLEKKKFKSFFFLCLALSIHLSSL
                     LIVSVIPIVWYLNRNIGNNIFNQIIIISLLLSVILLFDPYINLLRLVAEFPMMHERIA
                     PYLDGSYAVNNAPMFNSFVVIKIISYVIFFIWIYKNKKVGLTIENYLIYLCFFLSIVG
                     LFLFWCFRSNDSLSIRFSDFFAIYDIIFFALLLNVFDVFGKFIYRYYLLVMMIAFFIS
                     SFKLIN"
     gene            12764..13549
                     /gene="wcnZ"
     CDS             12764..13549
                     /gene="wcnZ"
                     /note="WcnZ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFW04786.1"
                     /translation="MNVYISVVSHNHAKMIIEFDCLRKLAKRYKVVIKNNSERESVIL
                     NDYCRKNEIYIIDNAYNTGFGKNNNIVFQYCEKELGMTSDDYFILINPDVIIDAININ
                     KLISIIEKDMIDISGISLYKDDALSIRDYSIRNYPSFYTFFMSFFRFYKKYCVLPPND
                     DCSSLDMDWVAGSFMVFKTKAYSSLLGFDEHYFMYCEDLDICFRAKSKGLNLKYIPSI
                     TGIHKAQHNNRRLFSKHFYWHVKSALRFLISKKIKLQVKSILK"
BASE COUNT     4311 a   2106 c   2809 g   4501 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcgtg ctggtgactc acgcgtctaa aaacgccgtt
       61 gagaaccact tcgacacctc ttatgaactt gaatcacttc ttgagcagcg cgttaagcgc
      121 cagcttctgg cggaagtgca atctatttgc ccgccgggcg taactatcat gaacgttcgt
      181 caggcacagc cgttagggct ggggcactct attctgtgcg cgcgtccggt tgtgggcgat
      241 aaccctttcg ttgtggtact cccggatatt attattgatg atgctaccgc cgatccgctg
      301 cgttataacc ttgcggcgat ggtggcgcgc ttcaatgaaa ccgggcgtag ccaggtgctg
      361 gcgaagcgca tgaaaggtga tttatcggaa tattctgtta tccagacgaa agaacctctg
      421 gataatgaag gcaaagtcag ccggattgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acactggact ctgatttgat ggcggttggc cgttatgtgc tttcagccga tatttgggcg
      541 gaactggaaa gaacagaacc gggtgcctgg gggcgtatcc agctcacgga tgccattgct
      601 gaactggcga aaaaacagtc tgttgacgcg atgctgatga cgggcacaag ctacgattgc
      661 ggcaaaaaga tgggctatat gcaggcattt gtgaaatatg ggttgcgcaa cctgaaagaa
      721 ggggcgaagt tccgtaagag catagagcag ctactgagcg agtagcgatt tacagatctt
      781 tgtgacggta aaccagaaaa aataacggca gctaacatcc ctatgtctat gctttatgct
      841 gtggggtgtt actgtcgttt tttattttaa aaacaaagaa taacaataag ttaatctact
      901 aattttaatc tgcttttgat gggatttttc cttgtttctg gccgcgtttg gtaagacaat
      961 tagcgtttga atttagcgtg ctttgcgaaa gctggcgggg ctggttacat ctttatccat
     1021 caagttgtgc tctggttgct gtaaggccag gggcggtagc gtgcgtaaat atccaggcgc
     1081 tggcattgta taccagcatt ttaatagtag tcatataaac gatattcgga attaaaacgt
     1141 gaaaatactt gttactggtg gggcaggttt cattggatct gcagttgttc gtcatattat
     1201 taaagatact caggataccg ttgttaatgt cgataaacta acctatgccg gtaaccttga
     1261 gtcccttact gaagtcagcg aaagtcatcg ctatcacttc gagcatgcgg atatctgtga
     1321 taaagcggca atggagcgca tttttgtcac tcatcaacca gatgcggtga tgcacctggc
     1381 ggcagaaagt catgttgatc gctctataac ggggccagca gcttttattg aaacgaatat
     1441 cgtcggtact tatgtattac tggaagccgc tcgtagttac tggaacgctc tggatgtgga
     1501 aaagaaagcg gcgttccgtt tccaccatat atccacggat gaagtatacg gcgacctgcc
     1561 acacccagac gaagtatctg ccggtagcgt gttgcctctg tttacggaaa agacagcata
     1621 tgcgccgagt agtccgtatt ctgcttctaa ggcatccagc gatcacctgg ttcgcgcatg
     1681 gttacgtacc tatggtttcc caacgatagt caccaactgt tccaataact acgggccgta
     1741 tcatttccca gaaaagctga tcccgcttgt gattcttaat gcgctggaag gtaaggcatt
     1801 gccaatctat ggtaaaggcg atcagattcg cgactggctc tatgttgaag atcatgcccg
     1861 agcactgtat accgtggtga cccaaggtgt agtcggtgaa acctacaata ttggtggcca
     1921 taacgagaag aaaaacctgg atgtggtaca aactatttgc gatctactgg atgagattgt
     1981 acctaaagaa ggggcttacc gcgatcaact gatctacgtc actgaccgcc ctggacacga
     2041 tcgtcgctat gcgattgatg cagcaaaaat cagcgatgag ctgggctgga aaccgcagga
     2101 aacatttgaa agtggtatcc gtaaaacagt ggagtggtat ttgaataata cccagtgggt
     2161 tgagaatgtg aagagtggca cttataaaac ctggattgaa cagaattacg gagagcgtca
     2221 gtaatgaata ttctcctctt tggcaaaaat ggccaagtgg gttgggaact tcaacgctca
     2281 ttagcgcctc ttggaaaaat tattgcgctt gatgtccact cgaaagatta ttgcggcgat
     2341 ttcagtaacc cagaaggcgt tgcggaaacc atacgtcgaa tccgcccaga tgtgattgtg
     2401 aatgcggctg cgcacactgc tgtagataaa gctgagtcag aagttgagtt tgcaacactg
     2461 ctaaatgcgc gaagtgtcga agctatcgcc aaagctgctt cagaaacagg ggcgtgggtg
     2521 gtgcactatt cgactgatta tgtctttcct ggtacagggg aaacaccatg gtgtgaagat
     2581 gatgctactg caccgttaaa tgtttatggt gaaacaaaac tgacggggga gaaagctctt
     2641 caggataatt gtgcccggca tctaattttc cgcaccagtt gggtttacgc aggtaaaggt
     2701 aataactttg caaaaaccat gcttcgtttc gcaaaagagc gcgaagaaat gtcggtgatt
     2761 aacgatcagt atggtgcgcc caccggagct gaattgctag cagattgtac ggctcatgct
     2821 atacgtgtag cgcttaaaca gcctgaggtt gcagggctgt atcacctggt ggctggtggc
     2881 gcgaccacat ggtatgaata tgcagcactg gtattcgatg aagcacgtaa agcgggaata
     2941 ggactggcaa ttaacaagct caatgctgtt cctaccagtg catatcctac ccctgcgcga
     3001 cgcccaaata actctcggct aaacactgat aaattccaga aaaaatttga tctggtatta
     3061 cccgagtggg agattggtgt aagacgtatg ctggctgaat tatttacaac tacgacagtt
     3121 taaaattatt ttaaatgccc ttaaatgggc attttgacat ataaagagat tatgaaatga
     3181 aaacgcgtaa agggattata ttagctggtg gctctggaac tcgtctttat ccggtgacaa
     3241 tggctgtcag caaacaactg cttccgatct atgataaacc aatgatctat tatccactgt
     3301 ccatgctgat gttagctggg attcgtgaca ttctgattat tagtactcca caggatactc
     3361 cacgatttga acagttactg ggtgaaggta gtcagtgggg attaaatctg caatataaag
     3421 tacaaccgac gccggatggt ctggctcagg catttattat tggtgaagag tttatcggta
     3481 atgacgactg tgctttagtg ctaggcgaca acatttttta cggccatgat cttccaagat
     3541 taatggaaac agccgtaaat aaagaaagtg gtgcaactgt atttgcttat catgtcaacg
     3601 atcctgaacg ttacggtgtt gtagagttcg acaaagatgg cacagcgatt agcctggaag
     3661 aaaaaccact tgaaccaaaa agtaattatg cggtgacagg gctgtatttc tatgacaatg
     3721 atgttgttga aatggcaaag aacttaaaac cttcagcacg tggcgagttg gaaattacag
     3781 acattaaccg aatttacatg gagcaggggc ggttatctgt tgcaatgatg ggacgtggct
     3841 atgcctggct ggatacaggt actcatcaaa gcttaatcga agcaagcaac tttattgcga
     3901 caattgaaga acgtcaggga ttaaaagttt cttgcccaga agagatagca taccgtaaag
     3961 gctttattga ctcggaacag gtgaaaaaat tagccatccc attgtcaaaa aatgcttatg
     4021 gacaatattt gattagaatg gttaaaggtt attaatatga aagttatcaa gactgatatt
     4081 cctgatgtgc tgatttttga acctaaagta tttggtgata aacgtggctt tttcatggaa
     4141 agttttaatc agcgagtctt cgatgaagct ttagggcaca aagttgagtt tgttcaagat
     4201 aatcattcta agtctagtaa aggcgtgctt cgtggcttgc attatcaact tccaccatat
     4261 gctcaaggaa aattagttcg ctgtgtagta ggagaagtct ttgatgttgc tgtggacatc
     4321 cgtaaagatt ctgaaacttt tggtaaatgg gttggtgtaa atctttctgc tgataataaa
     4381 aaacagttat ggatacctga agggtttgct catggatttt atgtattgag tgaaactgct
     4441 gagcttttat ataaaacgac taattattat aatttactat cagatcgagg gatcatttgg
     4501 aatgatagaa gtataaatat aaactggcca atctccggag atatacttct ttctgaaaaa
     4561 gatatgaatc atggaactct tactgaaaca tccaatatct gatattataa cttacatttc
     4621 gagagatgat taatgaaact agaaaatata aatattggta tagtcggttt aggttacgta
     4681 ggcttaccac ttgcggtaga attcggtaaa aaatttgaaa caattggttt tgatataaag
     4741 agcacaaggg tagaagagct aaaaaataat atcgatacaa ctttagaatg ttcaagcgat
     4801 gaacttcagt cggcgaagtt attgcaattc acaaataata ttgaagatat taaaaaatgc
     4861 aatgtatata ttgtgactgt gccaaccccc atagataaat tcaaacgacc tgatctttca
     4921 ccattagtca aagcatcaaa attattaggt tcaatattga ataaaggtga tgttgttata
     4981 tatgagtcaa cagtatatcc gggagcaaca gaagaagatt gtgttcctgt attagaacaa
     5041 caatcaggaa tggtttttaa taaagatttt tttgttggat atagtcctga gagaattaat
     5101 cctggtgata aagaacaccg tgttacctca ataagaaaag tcacatctgg ctcgaccact
     5161 gaaattgctg attttgtaga ttctatatat gcatctataa tcaaagctgg gacttataaa
     5221 gcaagttcaa taaaagtagc agaggcggct aaagtaattg agaatactca gcgtgactta
     5281 aatattgcgt tgattaatga attagctatt atatttaata aattaaatat tgatactgaa
     5341 gaggtactaa aagctgctgg taccaagtgg aactttttgc catttagacc aggacttgta
     5401 ggtggtcatt gtattggagt tgacccttat tacttaacac ataaagctca gtctatagga
     5461 tataatcctg aagttatatt atcaggaaga agaattaatg atgctatggg tgagtatgtt
     5521 gcgtcacagt tagtaaaaaa aatgataaaa acgaaaattc aaattgatta tgcggatgtc
     5581 ttagttatgg ggtttgcatt taaagaaaat tgtccggact taagaaatac taaagtgata
     5641 gatattataa gctctttaaa agattataat atcaatgcag atgtttatga cccttggatt
     5701 tcgccagatg aagcagtacg agaatatggg gttaatattc ataataatat acctgaaaaa
     5761 aaatacgatg ctattttatt tgccgtagct catgatgaat ttaaaaaaat gacgatgggt
     5821 gagattctat cgttaacaaa aagtaattat tttatatacg atctaaagta tattatagat
     5881 tctgacttgg ttattgaacg cttgtaacaa tatatatcga ctctgaggac attaccgtga
     5941 actatgatga gttgcaagac tatctattaa ataatcaaag aacttggtta attactggtg
     6001 tggctggatt tataggttca aacttacttg aaaagttatt aaaactaaac caaaatgtta
     6061 ttggcttaga taatttttcc actggttttc aatcaaattt aaatgaagtt aaagataatg
     6121 tatccgacgg ctcctggaga agatttaaat ttattgaagg agatattcgt gatttggacg
     6181 tttgcaaaaa aagcgttagt ggtgtcgatt atgttctgca ccaaggagca cttggatcag
     6241 taccaagatc aattgagaat ccaatattga ctaatgcttc aaatgttagt gggtttttaa
     6301 atattttaga ttgtgcaaga agagaaaatg ttaaaagctt tacttacgct gctagtagtt
     6361 cgacatatgg cgatcataca ggcctgccta aagttgaaga tattatagga aacccacttt
     6421 ctccttatgc tgtgacaaaa tatgtgaatg agctatatgc aggtgtctat gcattaaatt
     6481 ataatttcaa aagtataggg cttagatatt ttaatgtatt tggtagaaga caaaatccta
     6541 atggggcata tgcggctgtt attcctaaat ggatactttc aatatcaaat ggtgatgatt
     6601 tatatattaa tggtgatgga agtactagta gagatttttg ttatatagat aatgtggtcc
     6661 aagctaactt attagcggcg cttgcaaatg ataacataaa gaataatgta tttaatattg
     6721 cttatggaca acaaaccagt cttaataagc tatttgagta tattgcagga gctctagata
     6781 atgaaggaat cgaatacgat aagcaacctg tatttaagga tttccgaaaa ggggatgtaa
     6841 gacattcgct cgctaatatt gataaagcta aaaaattttt aggatatgaa ccatgctatg
     6901 atattaatga aggaataaaa ctagcgatca agtggtatat aaataatctg tccaaataat
     6961 atatggtaat tgtagactcg caaattaatg cgagtctaca gttattataa taaaaaagga
     7021 ttgcaacata ctatataatt tgattagaac ttaatattag taaaaaactc acatgtactt
     7081 aaaaatatta attgataatt tttcgcttgt aatccaatat tttttcggtg gcatagctgt
     7141 tttttatgtt acacctttaa ttgtgaaatc cgttggtatt cattcgtacg gaaatttagc
     7201 aattatgttt gctattgtca cttacatatc actaataata cagtactcct ttaatttgat
     7261 tggaccaaaa ttaatcgctg aagggaattg taaaaaaaca tttaatataa tattttcggc
     7321 taaacttgta ctttttatat tgtcacttgc tattttttta ttatattttt tttgcttcca
     7381 aagcaagtat gataacgtat ttctattatt ctttattttg ccactatctt gggttttaaa
     7441 taactcttgg ttccttcaga gcaaaggcta ttttatctta agtagcatat gttcaataat
     7501 aggatccttg gttacatttg ttattgcata tttttttata aatgcaaccg ttggtttgtt
     7561 agtgccaatc ttatgcctga ttttatcttc ctttataaac ggttccctta cattcttctt
     7621 tgcattaaag aaaattggat atgttaaaat aatcaatcca ctgcctattt tgaaaacagg
     7681 atttcctctt tttgcttctc agatagtttc ttcagtatat accatgtctg gcgttattat
     7741 aataactcat ttttatggtg ttacatctgc tggtacatat gctgttgtag aacgttttat
     7801 gaatttattg atttcgttag gtattcttac acatgtagca gcttatccaa aattggctag
     7861 attattcaat aaggacagat tggaatatag aaagacatta ttatttgttg tcgcattata
     7921 tacttttttt tcttgttgtg tggcattggg tgtttttaat tttcatcaag atattgtgaa
     7981 ctatatgttt ggaagtgaaa atggaaatgc taacgaacta atttattctg cgtgtatata
     8041 tatatttgtt tccatatatg ggcctgtggt tacagggtat ttggcattga aaatggaagg
     8101 gaagttaata ataataatca atatagccat tgcattactt tctttattgg cgggagttgg
     8161 aatgttaaaa ataatgggga atagtgggtg gttaatagga ttgagtcttg ctcagttact
     8221 ttatattatc atattcttta aaatatttat ttggggaaga aaagaatgtg tggtctagtt
     8281 ggctatttca gtagatctaa ttgcgatgcg aatataattc ataatatgtt gcataaaatt
     8341 agacatcgtg gacctgattc atttggggtc tgggaaagct tagaatgcaa tatccatttt
     8401 ggtcatgtta gattatcgat tgttgaattg agtagcgcag gccatcaacc aatgtcaact
     8461 tcctgtggaa ggtttactat catatttaat ggtgaaattt acaaccacct cgatatacgt
     8521 aaggaactgg gcaatgagat aaaatggacg ggaacatctg atactgaaac cttgcttaaa
     8581 agcatttcta cttggggggt ttctggtaca ttgaataaag cggttgggat gttttccttt
     8641 gcattgtggg atagtgtaga acggagttta tatctcgcga gggatagaat tggtgaaaaa
     8701 cctctctact acggctggtg taatggttct ttcttatttg gctcagaatt aaaagcttta
     8761 aaatcacatc ctgattttga tgctgaaata gactgggaag cagttaatgg atatttgcat
     8821 aataactata tctcttcacc cttaacaatt tatagcaagt taaaacaact gcgaccaggc
     8881 cattacatta aaatgagcca tgatgatctt ctttcaggtg atacacctgt attgtacaag
     8941 tattggtcat tatcttttcc tgtcagtgag aataaacaca ggtatgttga ttctgaatca
     9001 aaattagaag ctttactatc agaatcagtt tctcttcagt cgattgcaga tgtcaaagtt
     9061 ggagcttttt tatcaggtgg aattgattct acaaccattg tagcaatgtt gaaaaaaagt
     9121 ggacgtgatg tatctacatt ttccatcgga atgcctaata aacaatatga tgaatcacgt
     9181 catgccgaag acatcgcaaa atatattggt acgcaacatt atactcatat gataacacct
     9241 caggaagcgc tggaggtaat tgataaaatt cctgcaattt gggatgaacc tttcgcagat
     9301 agttctcaga taccaacata tttagttagt aagtttgcta aagagcacgt aacagtcgct
     9361 ttgtctgggg atggcggtga tgaactattt atgggataca atcaataccc tctgttgaga
     9421 aaaatttggg atacaaggta tttatcaaat ttacatttag atactatggc caagataata
     9481 acaaaaatgg gatttaagca ttccagtata attgctagaa agattttaaa tttaagccaa
     9541 ggttggcgtt gtaagacccc ttttttatta aatgattttt ggatggataa gtatcgtaat
     9601 acagaatttc ctttattaca gcctgttcct tataaacgga atttagactt aaattattta
     9661 gatggaattt cagcaataac tcagcatgat ttaaattatt atctttgtga tgatatatta
     9721 actaaagtgg atagagcttc tatggcggtt agtttagaaa cacgagcgcc atttttagat
     9781 caccgtattg ttgagtttgc atttggtcta ccgacatcat tcaaaatgga taaatacaat
     9841 cagaaaaaaa tactcaagtc agtactatat aagcatgtag atagtaagtt attagaacgt
     9901 cctaagcaag gtttttcatt accaatgaat caatggttaa gagccgagtt aaaaggatgg
     9961 gcacgagatc gtcttgattc tttaccagaa ggtgttttcg ataaaattgt tgttgataat
    10021 ctttggaaag aacatattga tgatgtcaag gataatagcg agcgaatttg ggcgctaagt
    10081 aatttagcta attttttgga gtcccatgaa taagattgct atatatactt gtattacagg
    10141 cggctatgat tttattaagg ctccacttaa aattaatgat gatatagact atatatgttt
    10201 cagtgaccaa aaaatccctg catcctaccc ttggaaaatt ataaatatat cagatttaga
    10261 aatatcggag ggatttgacg agaaaacaat aaatcgtgct ataaaaatat gtcctcaaaa
    10321 atttggttta ttagaacaat atgaactcac tatatatata gatggttcaa tcgaaattat
    10381 tgatgatctc tctttactaa ttgattttgt agctaaacaa gatgacgtca ttttcatgta
    10441 tgaccatttt ttaagaaatt gtttgtatga tgaagctgaa gagtgtcttt taattggata
    10501 cgactggtat tggaatattc gaaaacaagt tagaaattat aggcaaaaag gttttccagt
    10561 ttcatatggc ctctttgagt gtggaattat cataaggaaa aaatctcaag acttaaatat
    10621 cataatgtgg aaatggcttg aagaatacat aaaaggagtt aaacgtgatc aactttctct
    10681 tacttacata ttatgggaga gtaactatca tttgtgttct ttgggggaaa gtgacgctag
    10741 atataaaaat agacacttta agttacataa gcattcaata aaaagtggtt attattttag
    10801 gaaatttaga tctaagctca ataaggtttt aataagaatt tttggaggtt tataaagtgt
    10861 acgaaaaagt ttgtggtgtt gttattgtat tttatcatcc aaatgatgaa aatattaata
    10921 gtgcgaaaaa attaagtgag ctttataaag taactatcgt tgacaatagt gaaaaagata
    10981 taaattattt tatacccaag gctcatatta taaaattgaa tagaaacgta ggtatcgctg
    11041 ctgcattaaa cattgggata aattttttta tcgaaaatga ctataaatat gttattttat
    11101 tagatcaaga cagcgaaccg gatacattgc ttttgagttc acttatcaat tattctgaaa
    11161 gatgtacgga tgatgtgtgt ttagttgcac catcctatta tgatagagct atagataaaa
    11221 atgctgaatt tatattatgt actgataaag gtattattag gcagccagct attggaaaaa
    11281 atgcgattga agcatcctat gttataactt ctggatctgt cttgagactt tcatctgttt
    11341 ctaatattgg actaatgaac gatgatttgt ttatagattt tgttgatatt gagtggtgtt
    11401 taagagcaaa ttctttggga tataaaatct taggtttacc atggctaaaa atgtcacatg
    11461 agatcggcga taaacctatt aatatattta ataaaaaata tgttaaccat tccccaataa
    11521 ggcactacta ctactttaga aatatatttt tattgatgcg catgagtcat atccaccctc
    11581 aatggaaaaa atgggaactg ataaaactat tacctagatt ttttgtttat gcatgtttta
    11641 caaaaaataa tatgaaacat atttcctata tgttaactgg tatatatgat gggttacttg
    11701 ggcgagtggg gaaaaagaaa tgaatggagt agccaaaccc attataattt ttttgttcgc
    11761 ctgttgtact cttgtgactt ttctttcatg taatttaaat ttatttcctg attattattc
    11821 atatctaaat tggtatgagt tatcagcatc cacaaatatg aataatgatt gggtcttttt
    11881 taaagatcct ggattttatc tgttgtcagt tatatcaaat aattttggct ttggtgtcat
    11941 tggagtgatt ttttcgcttt ttataatatc actttcgtct aagatttttt tttgtattaa
    12001 gttacttaat agagaaactc tattctgggt gctactttta tatctgtcta ggctctttat
    12061 tgttcatgat cttgttcaat atcgagccgg tgcgtcaatt ggtttgtcag cattattttt
    12121 gtatttttat ttagagaaaa aaaagtttaa gtcttttttt ttcttatgcc tcgcactttc
    12181 tatccatctt tctagtttgc tgattgtgtc agttatccct atagtgtggt atcttaatag
    12241 gaatataggt aacaatatat tcaaccagat aataataata agtttactat tgtctgtgat
    12301 tttacttttt gacccttaca ttaatttgct aaggttggtc gcagagtttc ctatgatgca
    12361 tgaaagaatt gctccatatt tggatggttc atatgctgtt aataatgcac caatgtttaa
    12421 tagctttgta gtaattaaaa ttatatcata tgtaattttt tttatatgga tctataaaaa
    12481 taaaaaagtt ggtttaacca tagaaaacta cttaatttat ctatgtttct ttctgtctat
    12541 tgtaggattg tttctttttt ggtgtttcag aagtaatgac tcgctatcca ttagattttc
    12601 tgactttttc gccatatacg atattatttt ttttgctcta ttattaaatg tttttgatgt
    12661 gtttgggaaa tttatctata gatactatct tttagttatg atgattgctt tttttatatc
    12721 ttcatttaag ttgataaatt agtttctgat atgtgtgata attatgaatg tttatatatc
    12781 tgttgtctct cataatcatg ctaaaatgat tattgagttt gattgtttac gtaagcttgc
    12841 taaaagatat aaagtagtta ttaaaaacaa ttctgaacgc gaaagcgtta ttttgaatga
    12901 ttattgtagg aagaatgaaa tatatataat tgataatgcc tataatactg gctttgggaa
    12961 aaacaataac atagtattcc aatactgtga aaaagagtta gggatgacaa gtgatgacta
    13021 ttttatcctt attaaccccg atgttataat tgatgcgatt aatatcaata agcttatttc
    13081 cataatcgaa aaggatatga ttgacatctc cggaatatct ttatataaag atgatgcctt
    13141 gtcgataaga gattactcaa tacgtaatta cccttctttt tacacttttt ttatgtcgtt
    13201 cttccgattt tataaaaaat attgtgtatt accgcctaat gacgattgct ctagtttaga
    13261 tatggattgg gtcgcgggtt cttttatggt ttttaaaaca aaagcatact caagtctatt
    13321 aggcttcgat gagcattact tcatgtattg tgaggattta gatatttgtt ttagagctaa
    13381 gagtaagggc ttaaatttaa agtatatacc tagtatcact ggaattcaca aggcccagca
    13441 taataataga agactattta gcaagcattt ttattggcat gtcaaaagtg cgctaaggtt
    13501 tttaataagt aaaaaaatta aactgcaagt gaaatccata ttgaaatgat accttaatat
    13561 tatcggagtc ttaaaagatg tcatcgatag gcatcgttca ctattcatta atatcaattt
    13621 taggttagaa ttgtcactag atagccgcgc ataccctgcg cggtgaccac acctgacagg
    13681 agtatgtaat ggccaagcaa cagatcggcg tcgtcggtat ggcagtg
//
