LOCUS       GU299799                7618 bp    DNA     linear   BCT 30-SEP-2010
DEFINITION  Salmonella enterica strain G1601 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299799
VERSION     GU299799.1
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Liu,B., Perepelov,A.V., Li,D., Senchenkova,S.N., Han,Y.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Structure of the O-antigen of Salmonella O66 and the genetic basis
            for similarity and differences between the closely related
            O-antigens of Escherichia coli O166 and Salmonella O66
  JOURNAL   Microbiology (Reading, Engl.) 156 (PT 6), 1642-1649 (2010)
   PUBMED   20185508
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Liu,B., Perepelov,A.V., Li,D., Senchenkova,S.N., Han,Y.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="G1601"
                     /db_xref="taxon:28901"
                     /note="serogroup: O66"
     misc_feature    1..7618
                     /note="O antigen gene cluster"
     gene            153..1409
                     /gene="wzx"
     CDS             153..1409
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADC54981.1"
                     /translation="MHSKLKMKKLLDNAIVKNILWLISEKLYFVILIFFGEGLISRTL
                     GVNDYGKWIYSLNIIIIISSVALIAGSEVMVPALSKNKKLAGELITSAFIIRFSGALL
                     SFLVLNIYSLVLVNDLVIQHMLLSLSLVLLFNEPFGAITNYFQAKIDMKKVVLARCLS
                     LALRTIFVLVAFKIFESNLIYFSRASESILLALILSLLLYKSKIRLNFSKKVFLIVLK
                     RGCLLWVPLISMMIYLRIDRFFVEKYFSYETLAMYGVAVQFIEQAFLLLVIVIQTISP
                     KYIFQSMPKKTMLKNIKLISLLLIMLVFMMQVFCLFFLKSIITIVYGDSYIQAATLAI
                     SLLPSLLFYAVDTILMQVFYKYRNNKAILTKWISMMFFSCAMYYIWFDLLSKSNVTMV
                     FNINYLTMSVITFVLFRRGLKDCKND"
     gene            1402..2214
                     /gene="weiA"
     CDS             1402..2214
                     /gene="weiA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADC54982.1"
                     /translation="MIKFTIFTPTYNRKNYLERCYRSIVSQNFESLEWLVIDDGSNDG
                     TKELIYKFISEKKIDILYIYQENAGKQAAWNLALEKARGQYFIGLDSDDALNEDCLDA
                     VINDVDIISSNESVIGLRCLSKKIPGSHDSDNNFSSDSDRICSWFEEFASGIYQERID
                     IFKTSMLKNYYYPIEEGMKFIPEIWLYSLISKKYNFFYVNKIVRVFYDDDSHNRLSKS
                     SFRKHYKGHLLARKSMLKNIPLKYLLKNPLGLIKTILRYMQAIYCSFLFRGN"
     gene            3089..4183
                     /gene="weiB"
     CDS             3089..4183
                     /gene="weiB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADC54983.1"
                     /translation="MKKVCFFVGDISRSGGTERVTCIIANYLAQFDFEIHITSIYGTN
                     KSYFPLDLKVKRNTLFETQRRGMVNFPFVIIKLRRFIKQNNIESLISVESMLSLYSIP
                     ATANLNIQNICWEHFNYKINLNKKSRSLARYLAARYCDTVITLTERDKMYWEANLKCR
                     ATIKRIYNPVPFVNNNLDFSSSPRIALAIGRLTHQKGFDSLIKIWAQTYPHNKEWVLK
                     IVGNGEDELYLKQLCQNYGIKNIDILPATKDIDKIYKEASLYLMTSRYEGFPMVLLEA
                     QEYGLPILSFDCETGPAELIDNNSTGWVIEDQDHFAFEKKLIHIINLFDNDHEFYCKI
                     RENSYRNSEKFCIDIIGPVWIDLLKKKGAQ"
     gene            4180..5280
                     /gene="weiC"
     CDS             4180..5280
                     /gene="weiC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADC54984.1"
                     /translation="MKNICLVTTSLGMGGAEKQVCDLADSFYKEGHKVIILCLNNSVI
                     NNPVNDDINIHVLDLKKNIVSFCRCIFQARKILKEFKPQIVHSHMFHANIFSRILRLL
                     CPFPVLISTAHNTNEGGRLRMCLYRVTDFLTTFSTNVSNEAVESFINKGAVKKGRMVA
                     FYNGIDTNLFKYSLESRINKRRELKLKEEDVLMLAVGRLTEAKNYKNMLNALQRVIAV
                     KVVHLAIIGTGPDEQQLLSYAEQIGVSQYIHWLGIRFDVKEWMSAMDLYVMSSSWEGM
                     PLVICEAMSCEGLVVATDCGGVKEIVGNAGFIVPTNNPDALSETILSALELTKTKRKR
                     LGITARERIETHYSLDTISNKWLEFYETANKK"
     gene            5258..6334
                     /gene="weiD"
     CDS             5258..6334
                     /gene="weiD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADC54985.1"
                     /translation="MKQLTRNDLKLVPKNIVIIISKSEIGGAQTWVLHLKEILESEYN
                     IFLVTSDYGWLTGFFDPKRVVIIPGLASMRRPDTIFKIRSALKKFNAHFVISNSASAG
                     LYSRIAKLIFQHKHIYVSHGWSCLYNGGRLKFVFCFIEKILSVFTDKILCVSNNDQYK
                     AINLIGISPTKTIVIRNGAKPCEKKQFVNDVKRILFLGRMIHPKRPDLLAEVALNFPD
                     VNFYFIGDGPLLSELKNSYKYVKNIFFLGEIRNFINFKEYDLFVLSSDSEGLPMSAVE
                     AATANLPLLLSNVGGCGELLISNSEGYKNGLLFNNELTDLTEKLQIILNDYTNYYKAA
                     KDISEQFEITNNKNLYLNLIQSLR"
     gene            6439..7464
                     /gene="gne"
     CDS             6439..7464
                     /gene="gne"
                     /note="UDP-GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADC54986.1"
                     /translation="MAILVTGGAGYIGSHTTLALLEAGYQVIIVDNLTTSHFDSVLRL
                     QKLAGEVIDFYVGDIRDKHFLHSVFSSNNIESVIHFAGLKSVGESVVLPIKYYDNNVS
                     GTLNLISEMIQHNIHHLIFSSSATVYGNPEKIPLEECSRIGGTTNPYGTTKLMVEQIL
                     DDVTAVYPEFRTTILRYFNPVGAHPSGEIGEDPNGIPNNLMPYICQVAIGKHKQLSVL
                     GSDYPTKDGTGIRDYIHVMDLAEGHVAALERRNEGPNHKVYNLGTGTGYSVLEVLNAF
                     ERVTSYAVPYILSERRPGDIAECWSDPTKAQKELGWKAQRGLEDMIRDAWNWQQKNPN
                     GYKKPDS"
BASE COUNT     2538 a   1018 c   1416 g   2646 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg ttgtttgttt agttaacaat tataatacta
       61 taatgacttt attaaataca ttcgttgaaa gtttcaataa gtatgaaaat ttaaatcgtc
      121 aatggtgctt cttaaattag caagagaaat taatgcatag taaattaaaa atgaaaaaat
      181 tattagacaa tgctattgtt aagaatatct tgtggcttat aagtgagaag ctatatttcg
      241 ttattcttat tttttttggc gaggggctta taagtcgtac acttggagtg aatgattatg
      301 gtaagtggat ttattccttg aacataataa ttattatatc ctcagttgca ctaattgctg
      361 gaagtgaggt catggtgccg gctttgagca agaataaaaa attagcaggg gaattaataa
      421 cttcagcttt cataattaga ttctcaggcg cattactatc atttttggtt ttgaatatat
      481 actctcttgt gttagtaaat gatttagtga tacaacatat gcttctttct ctttcgttag
      541 tgcttttatt caatgagcct ttcggtgcga taactaacta ttttcaagca aaaatagaca
      601 tgaaaaaagt agttctggct cgctgcttat cactagcatt gcgtacaata tttgttttgg
      661 tcgcatttaa aattttcgaa agtaatctca tttatttttc aagagccagt gaatctattc
      721 ttttggctct aatactatca ttgcttttat ataaaagtaa aataaggctg aatttttcaa
      781 aaaaagtatt tttaatagta ttaaaaagag ggtgtttatt atgggtccct ctaattagta
      841 tgatgattta tcttagaatt gacaggtttt ttgttgaaaa gtacttctct tatgagacgt
      901 tagctatgta tggcgttgct gttcagttta ttgaacaggc atttttatta ttagttatag
      961 tgattcagac aatttcccca aaatatattt ttcaatcaat gccaaaaaaa acaatgttga
     1021 aaaatataaa gcttatatct ttattgctca ttatgcttgt ttttatgatg caggtctttt
     1081 gcttgttttt tttgaaaagt attattacca tagtatatgg ggattcatat attcaagcag
     1141 ctacgttggc tatctcgtta ttaccttcat tattatttta tgcagtagat accattctta
     1201 tgcaggtgtt ttacaagtat aggaataata aagcgatact aactaaatgg atttcaatga
     1261 tgtttttctc ctgtgcgatg tattacattt ggttcgactt gctttcaaaa tctaatgtaa
     1321 ctatggtttt taatattaat tatttgacta tgtcagtaat tacatttgtt ttatttagac
     1381 gtggattaaa agattgcaaa aatgattaaa tttacaatat ttacacctac atataatagg
     1441 aagaattacc ttgaaagatg ttatagaagt attgtaagtc aaaacttcga gtcactagaa
     1501 tggctggtta tagatgatgg ttctaacgat ggaactaaag aattaatata taaattcatt
     1561 agcgaaaaga aaattgatat tttgtacata tatcaagaga atgcaggaaa gcaggctgcc
     1621 tggaatttag cgcttgagaa agccagaggg caatatttta tagggcttga ttcagatgac
     1681 gcattaaatg aagattgcct cgatgctgtt attaacgatg tggatataat atcatcaaat
     1741 gaaagcgtta tcggtctaag atgtctatca aaaaaaattc ccggttcaca tgatagcgat
     1801 aataattttt catcagattc tgacagaata tgttcatggt tcgaggagtt tgctagtggt
     1861 atatatcaag agcgaattga catttttaaa acatcaatgt taaaaaatta ttattatcct
     1921 attgaagaag ggatgaaatt tattcctgaa atatggcttt atagcttaat ttctaaaaaa
     1981 tacaatttct tttatgtaaa taaaattgtc agggtttttt atgatgacga ttctcataat
     2041 cgacttagca aaagttcttt caggaaacat tataagggcc atcttttggc aagaaagagt
     2101 atgttaaaaa atattccgtt aaagtattta cttaaaaatc cattgggatt gatcaagact
     2161 attttacgct atatgcaagc tatatattgt agtttcctgt ttaggggaaa ttaatattgt
     2221 tatagttgag tcttgctatt aagttatttt atggttataa aaatttaatt atttataagg
     2281 cattaaaaat gccatgtcaa aaatataaaa tattatatca aaccttcaaa agaagatagg
     2341 caattttata atcttgcgat ctttcttcgt agtaattata agttatttgt aataagagac
     2401 taagcaaagc agatttttca gtccagccct tatcctcacg ataaagacta tagtttctct
     2461 ctatgtaata agcgtttacg gggatagata tgtttaggtg actgtatata atgagttatt
     2521 aagattaaac aagacggggt aatacattcc tcaaatttat gattttttat tctcatgaag
     2581 attcctattt gttttagttt taatttattg tttcagtctt ttaagcgata gttttatgtt
     2641 ttattgattt tataaagagc acatgtaata ttaactttat tcttattgac ttattggata
     2701 taaaatgttt ttattttgct tagagaagag gctttacata aataatattt ttatttggtt
     2761 gttaaaagat tttaaattca aactattata gattaaaaaa gaacaatgcc cattaataac
     2821 ctttttgata agtggtgttc tggtatataa cacaatcttg gtcataacct tcatgaacgt
     2881 accaatagtg atgagaatat aactattatg ctttacatta atactaatat tgccagacat
     2941 aacatccaaa tattgtatta aagcaaatgt tatgaatact gttatggttc gatttggctt
     3001 tgcgattttt ccaactatct actactattc gagattatca tctaaagtag tatagtgagt
     3061 ataagataat attataaaag gattatctgt gaaaaaagtt tgtttctttg taggtgacat
     3121 aagtaggtcg ggaggtacgg agagagtaac ctgcattatt gccaattacc tcgcacaatt
     3181 cgattttgaa atccatataa caagtatata tggtacaaat aaatcttatt ttcctttaga
     3241 tctaaaagta aaaagaaata ctttatttga aacccaacga agaggaatgg taaattttcc
     3301 ttttgttatt attaagttga ggcgatttat aaagcaaaac aatatcgaat cattgatttc
     3361 cgttgaatca atgctctctc tttattccat ccctgctact gctaatttaa atattcaaaa
     3421 tatttgttgg gagcatttta actataagat aaacttaaat aaaaaatcga gaagtttggc
     3481 aagatatcta gctgctcgat attgcgatac ggtaattaca cttaccgaac gagataaaat
     3541 gtattgggaa gctaatttaa agtgcagagc aactataaaa aggatttata atcctgtacc
     3601 atttgttaat aataatctgg atttttcttc atcacctcga attgctttgg ctataggtag
     3661 attaactcat cagaaaggat tcgattcttt aattaaaatc tgggcgcaaa cttatccaca
     3721 taataaagag tgggtgttga aaattgtcgg gaatggagag gatgaactat atcttaaaca
     3781 actatgccaa aattatggca taaaaaatat agatattttg cccgcgacaa aagatatcga
     3841 taagatatat aaagaagcgt cattatattt aatgacttct cgatatgaag gcttcccgat
     3901 ggttctgcta gaagcgcaag agtatggact tcccatatta tcatttgatt gtgagacagg
     3961 gcctgccgaa cttatagata ataatagcac tggatgggtg attgaagatc aagatcattt
     4021 tgcgtttgaa aagaaattaa tacatattat taatttattc gataatgatc atgagttcta
     4081 ctgcaaaatc cgtgaaaatt cttatagaaa ttcagagaaa ttttgtattg atattattgg
     4141 tcctgtatgg attgatttgt taaaaaagaa aggagcacaa tgaaaaatat ttgtctggtg
     4201 actactagtt taggaatggg gggggctgag aaacaagtat gtgacttagc agatagcttt
     4261 tataaagagg gccataaagt tattatattg tgtttgaata attctgttat caataatcct
     4321 gtaaatgatg atataaatat tcatgtacta gatttaaaga aaaatatagt tagcttttgt
     4381 aggtgtattt ttcaggctag aaagatttta aaagagttta aacctcaaat agttcatagc
     4441 catatgtttc atgctaatat tttttcccga atattgcgat tactttgccc ttttcctgtt
     4501 ctgattagca ccgcacataa tactaatgaa ggtggtaggt tgaggatgtg cctgtataga
     4561 gttactgact ttttaaccac tttttcaaca aatgttagca acgaggcggt tgaaagcttt
     4621 ataaataaag gcgcagtgaa aaaaggacgg atggttgcat tttataatgg tattgataca
     4681 aatctgttca aatattctct cgaatcaaga attaataaaa gaagagagtt aaaattaaag
     4741 gaagaagatg tgctaatgct ggctgttggg cgtttaacag aagctaagaa ttataaaaat
     4801 atgttgaatg cattgcagag agtaatcgct gtgaaagtgg tacatcttgc aataattgga
     4861 actggccctg atgagcaaca attattatca tatgctgaac aaataggtgt atcacagtat
     4921 atacattggc tcggcattcg ctttgatgta aaagaatgga tgtcggccat ggatttatat
     4981 gttatgtcat cttcttggga aggaatgcct ctggttatct gtgaagcaat gagttgtgaa
     5041 ggtttagtag tggcaacaga ttgtggaggg gtaaaagaga tagttggtaa tgcggggttt
     5101 attgtgccaa ctaataaccc agatgcgtta tctgaaacga ttttatcagc attagaactt
     5161 acaaaaacta agagaaagcg tttagggata actgcaagag aaagaattga gacacattac
     5221 tctttagata ctatttcaaa taaatggttg gaattttatg aaacagctaa caagaaatga
     5281 cttaaagctg gtgccgaaaa atattgtaat tattataagt aaatctgaaa tcggaggagc
     5341 ccaaacttgg gttttacatt taaaagaaat tctagaatca gaatataata tttttttagt
     5401 aacgtcagat tacggatggt tgactggttt ctttgaccca aaaagggttg ttattattcc
     5461 tggtcttgca agtatgagaa ggcctgatac tatctttaaa attcggtctg ccttgaagaa
     5521 attcaatgcc cacttcgtta tttctaattc tgcttctgca ggattatact cacgcattgc
     5581 taagttgata tttcaacata aacatatata tgtttcccat ggatggtctt gtttatataa
     5641 tggagggcgt ctaaaattcg ttttttgttt tatagaaaaa attttatctg tctttacaga
     5701 taaaatatta tgtgtttcta ataatgatca atataaagca attaatttga taggtatcag
     5761 tccaactaaa actatagtta ttcgaaatgg tgctaaacct tgtgaaaaga aacaattcgt
     5821 taatgatgtt aaaaggattt tgtttttagg tcgaatgatt catcctaaac ggccagactt
     5881 attagcagag gtcgcattga actttcctga cgttaatttt tattttattg gtgacgggcc
     5941 tcttttatcc gagttaaaaa atagctataa atatgtaaaa aatatttttt ttcttggtga
     6001 aataagaaat tttattaact ttaaagaata tgatcttttt gttttatcat cagatagtga
     6061 aggattaccg atgtcagcgg ttgaagctgc tacggctaac ttacccttac tgcttagcaa
     6121 tgttggtggt tgtggtgaac tattaatatc gaattcagag ggatataaga atggtctatt
     6181 attcaacaat gagttaacgg atcttacgga aaaactacaa attattttaa atgattatac
     6241 aaattactat aaagctgcaa aagatattag tgaacaattt gaaattacta ataataaaaa
     6301 tctgtatttg aatttaatac aaagtttgcg ttgaaatttt ataaaatcca ttaattattt
     6361 ctccaagttt attgttattt aataatagct agcttgatct cagtgtttaa ttgattttga
     6421 tgaaagttag gggggcttat ggctatttta gttacaggtg gagctggtta tataggttca
     6481 catacgacat tagcgttact cgaagccggg taccaagtga taatagttga taatttaact
     6541 acttctcact ttgattcagt tctgaggttg caaaaattag caggggaagt tatagatttt
     6601 tatgttggtg atataaggga taaacatttt ttacatagtg tttttagcag caataatatt
     6661 gaaagtgtca ttcactttgc tggcttgaaa tctgttggtg aatctgtggt actgcccata
     6721 aaatactatg ataataatgt gtcaggaacg cttaatttaa taagtgaaat gattcagcat
     6781 aatatacatc atttgatctt tagttcttct gctactgttt atggcaatcc agaaaaaatt
     6841 cctcttgaag aatgttctag aataggggga actacaaatc cttatggaac taccaaactt
     6901 atggtcgaac aaatattgga tgatgtaacc gcagtgtatc ctgaattcag aactacgatt
     6961 ttgagatatt ttaatcctgt cggcgcacat ccgtctggag aaattggtga agaccctaat
     7021 ggtattccga acaacttaat gccttatatc tgtcaggttg ctatcggaaa acataagcaa
     7081 ctgtctgttt tgggaagtga ctatccaaca aaagacggaa ctgggattcg tgattatatt
     7141 catgtgatgg atcttgccga aggtcatgtt gcagctttag agcgcagaaa cgaggggcca
     7201 aaccataaag tctataatct cgggacggga acaggatatt cagttctgga ggttttaaat
     7261 gcttttgaac gtgtgacctc ttatgctgtt ccttatattt taagtgaacg gcgcccaggt
     7321 gatattgcag agtgttggtc tgatcctact aaagcgcaaa aagagcttgg atggaaagcg
     7381 cagcgtggat tggaagatat gattcgagat gcatggaatt ggcaacaaaa gaacccaaat
     7441 ggctataaaa agcctgatag ctaaaggagt ttttcgcaat agtataaatt atccatttta
     7501 tgtatcctga gttaacatct agaccacatt tcaagccgtg ccctcgtcgc gcggtgacca
     7561 cacctgacag gagtaaacaa tgtccaagca acagatcggc gtcgtcggta tggcagtg
//
