LOCUS       AY693427               13403 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Shigella boydii serotype 10 O-antigen gene cluster, partial
            sequence.
ACCESSION   AY693427
VERSION     AY693427.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Senchenkova,S.N., Feng,L., Yang,J., Shashkov,A.S., Cheng,J.,
            Liu,D., Knirel,Y.A., Reeves,P.R., Jin,Q., Ye,Q. and Wang,L.
  TITLE     Structural and genetic characterization of the Shigella boydii type
            10 and type 6 O antigens
  JOURNAL   J. Bacteriol. 187 (7), 2551-2554 (2005)
   PUBMED   15774901
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   Senchenkova,S.N., Feng,L., Yang,J., Shashkov,A.S., Cheng,J.,
            Liu,D., Knirel,Y.A., Reeves,P.R., Jin,Q. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2004) TEDA School of Biology Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="10"
                     /db_xref="taxon:621"
     gene            1..765
                     /gene="galF"
     CDS             1..765
                     /gene="galF"
                     /note="UTP-glucose-1-phosphate uridylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="GalF"
                     /protein_id="AAW29812.1"
                     /translation="MVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLA
                     EVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRY
                     NLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDCEGKVSRIVEFIEKPDQPQ
                     TLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSY
                     DCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1098..2066
                     /gene="wbaS"
     CDS             1098..2066
                     /gene="wbaS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAW29813.1"
                     /translation="MDDSVSVVIPYYNDSPRIEKCLDSICSQTRQAIEVIIVDDCSKD
                     SALLLKIIDKFKNKINIRYLRNDENKNGAYSRNVGMREARGGIVAFLDADDYWATDHL
                     EESVNALLLNGVEFVFSNVIEVDCCGCESRRKVTNPQYLDNKYDIILLSPPQTNSFLF
                     RKKIFDTKEIFFDENLRRHQDYQFLTLILEKNISYQYIDKYTSYYVQSHRPHTERMDY
                     KSVFLFWEKYNSYVSGRLLKKFITGLVLEVVLVYGKNKTKSFIDSHELVKINASKLFL
                     YLVLKTNFGVNANRVIYPFFYYILFSPKSILKKISQRINRKLVHNA"
     gene            2059..3168
                     /gene="wbaT"
     CDS             2059..3168
                     /gene="wbaT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAW29814.1"
                     /translation="MRNKISIVTTNITNNGGTERVIANVANSLSEIYDIRIYSLSTTE
                     GECFYPLNERIHIKHMGMMCYEHEKSVLKKTFKKLINVFYSINEFKKIRSNYIIGVNK
                     NINILLVLSFLLNYKRKRDYKLIGWEHFAHNAPMSLFTRILRELLYKYLDDLIVLTKF
                     DEEYYKAKKIKTTVIENAYTAMDVDNLKTVSKGNVILSIGRHTSQKRFDKLLHIWKEI
                     VKKNDGLKLRIVGDGPLLDENKKLAQKLNLSDSVVFAPPTKKINEEYQNASLFLMTSD
                     YEAFPMVLLEALKNGLPCIAFDCDTGPRDIIVNGVDGFVIPINDSLKFVEKTIDVMNN
                     ENLRYQLSSNAKVNVERFNEEHISKKWLIKLKQLQ"
     gene            3195..4247
                     /gene="wzy"
     CDS             3195..4247
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAW29815.1"
                     /translation="MIIITSFMVLLFLFTDNLKLKNFRIILFYFFSLCFSVLVYFRPE
                     YMLNTDYKEYYNYFKWINFDNIYNLSDTIGFEYGFGYIVTLIKYFFESERACFSGIAF
                     LSVFISYKAILRLNPKTTAFSFSLFLLSFCVYIFLGQIRQGISLSLGLLAISYLLEDK
                     RKQFLLTVIIASSIHVTALILILAPFVKYFRIKYIFYSLLLSFAFVFIDVIKPLIITL
                     AQFIPFGSFITGKIIAYGNSEFSTKVGFSFIQVYYVLLSTVLYILVKKYEYKNNYILT
                     LCKIFMVGVILNFTFNSFSVLLRLTYYYLALDCILMGYLLNMAKNGYTKILIYCPTLM
                     LFILRFYMQWSEHING"
     gene            4240..5331
                     /gene="wbaX"
     CDS             4240..5331
                     /gene="wbaX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAW29816.1"
                     /translation="MVEHCSKNKKILVDCRMINASGIGVYTREIIKELKKYRYLQIEL
                     LLQEEQQLPIDVEDIGFKIHYINFSRYSIKNIGGMKHLLNGFDVYFMPSLSIPPLLKK
                     GCLIITTVHDLCPVALRKLFGSKKAVAYWALLLIQLVCSNKIISISRFTAKEINKYYF
                     GLFKSKLYVIGNGIRNIFNLELNSETENSHEEYGICVGNIKPHKNVVPLIDYLKTNII
                     NKKIYFIGEINGFSTKINKSLLNDLPENIVFTGRVSDQELHNYYRNASFFIFPSLYEG
                     FGLPLLEAMNYRLPIFASNIDVFKEVAGETINYFDPYTFDALADRINKLKKNKKSIES
                     YARVLQLYSWDNNVASLLRIMNEEDIARK"
     gene            5306..6424
                     /gene="wbaY"
     CDS             5306..6424
                     /gene="wbaY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAW29817.1"
                     /translation="MKKILLVNKYYHPDIGGVETVVQQHAQLLKYEYSVTVLCISKEF
                     SWYTKNEFINGVKVIRCSPLGTYFSMPLSITFFLHYFINYLKSDVIINHIPFPLMDLA
                     FFIIDRLVKRKNILFWHSDIVKQKALKKILRPFIINTINRSTQILTTSPNLKANSEDI
                     GEYQKKTDVLPLFINSNVINGYIKNLNAEKKYDFIFFGRLCYYKGVDILLDAVKILKN
                     KGAEPEIFMAGDGDLVNYIENEIIENKIKNITFIKRFLTEEEKYHYLSISKCFLFPSV
                     AASEAFGITQLEAMSLGLPVINTNLNTGVPFVSLNGVTGITVTAGNPDDLANAMLSLL
                     RDDEKYESFKTNCKVRVSELFEENVVKNKLFKVIEELN"
     gene            6484..7920
                     /gene="ManC"
     CDS             6484..7920
                     /gene="ManC"
                     /note="mannose-1-phosphate guanylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="AAW29818.1"
                     /translation="MSNNIICPVIMAGGSGSRLWPLSRLFYPKQFLKLVDDSSMLQET
                     ISRLGDLPCSLPVVICNEQHRFIVAEQLRMINKLTNNIILEPVGRNTAPAIALAAMIS
                     ESFSSLEDPLLLVLAADHDIKNHKSFRDAVRKAIPFAEQGKLVTFGIVPSMAETGYGY
                     IKRGHEISLENDEVAYTVSKFYEKPNISDAQKFLASGKFYWNSGMFLFRASRYLSELN
                     KFRPDIYLACASSTKNINTDLDFIRVDENSFSNCPDESIDYAVMEQTSDAVVVPMNAG
                     WSDVGSWSSLWEISKKNEQGNVSHGDVINHNTENSYVYAESGLVTTIGVKDLVVVQTK
                     DAVLVADRNSVQDVKKVVEQIKAHGRSEHQMHREVYRPWGKYDSIDAGERYQVKRITV
                     KPGEGLSVQMHYHRSEHWVVVAGTAKVTIDGVIKLLGENESLYIPQGAKHCLENPGKI
                     PLELIEVRSGSYLEEDDVVRFADRYGRV"
     gene            8034..9404
                     /gene="manB"
     CDS             8034..9404
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="AAW29819.1"
                     /translation="MKKLNCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     SYINAKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESKANVELLESRVSII
                     LNILNM"
     gene            9468..10424
                     /gene="wbaM"
     CDS             9468..10424
                     /gene="wbaM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbaM"
                     /protein_id="AAW29820.1"
                     /translation="MNDIRDSINLLSKHNFDLVVGIPRSGMIPAYLISLYLNIDVTDV
                     NSFILNTPIQRGSTRTSGKRIYNPHDAQRILLVDDSFSTGKSMRNILDSIPVDLKKNI
                     KTMVAYTSDVNGAGLDIYIRHVSHPRLFEWNILNHSIISNSCFDIDGVLCVDPNELQN
                     DDGENYIKFICEAEPKFIPKFKIKYLVTNRLEKYRDITEAWLLKNNIQYEKLIMLNMA
                     TKEERQSAGIHSRHKAEFYKRSGCDLFVESDINQAIEIMKYTGKYVYCIDNNRMYSPS
                     AIQYLSKRPLDFMNDIILYLPRILYRRLPLETKMAIKKRLKP"
     gene            complement(10458..11870)
                     /gene="wzx"
     CDS             complement(10458..11870)
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAW29821.1"
                     /translation="MSIFNNLKWNAISQLFKIATQLISMIYLARLIAPEDYGIMAMAT
                     VVTNFAILFRDLGTSAAIVQRDSIDESLKSAIFWLNLLLGIIVGTAILFSSSYFSELY
                     DVPKLKPVLSLLSINFFLLGATSVHLSLLERESKFETISKIEIFSFGSALIIAIAMAY
                     LNFGVYSLVTQSIVSTLLSSGLFWYLSSWSPRIKIENIFKNTKSIFSFSYQLSLFNFI
                     NYFARNLDSFLIGKYMSATILGAYNLAYRIMLFPLTSITFIVNRSLFPILSKNKHKHA
                     EVEKIYLNTVYTIWFITIPLIISLLGLNEMVINIIFGEKWHLSSSILVWLAPTAIIQS
                     ILSTTGIIFMSQGRTGILVLLGVQGAILTGIAFVIGVNDNIISLAKYYFIANLVHFFP
                     CMALTMYVIKSNFYKLILKTYPLFIAGFISYYIIYILKKHLDHSFLSLVILTIICFVV
                     FIVSLLPTKEVKNFINRLIK"
     gene            12064..>13403
                     /gene="gnd"
     CDS             12064..>13403
                     /gene="gnd"
                     /note="6-phosphogluconate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="AAW29822.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRD"
BASE COUNT     4348 a   2224 c   2647 g   4184 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtgca gtctatctgt ccgcctggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cgcgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgcta
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagacaaa agaaccactg
      421 gattgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcaacc tggtgcatgg gggcgtattc agctgactga tgccattgct
      601 gaactggcga aaaaacagtc cgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactgcgcaa cctgaaagaa
      721 ggggctaaat tccgtaaagg gattgagaag ctgttaagtg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggtgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaatgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtctgaa ttttatgaat tactttggct ggtgccagag ttgacaaaga catgtagaat
     1021 aaaaagtgca ctggtagctt taagccaggg gcggtagcgt gtcaaatata tattttatta
     1081 ttgagtggct tacatatatg gatgattcgg tttctgtcgt tataccttat tacaatgatt
     1141 cccctcgtat tgaaaagtgt ttggatagta tatgttcaca aaccagacaa gctattgaag
     1201 ttataatagt tgatgattgt tcaaaagata gtgcattact actaaaaatc attgataaat
     1261 ttaaaaataa aataaatata cgatatttga gaaatgatga aaataaaaat ggggcatatt
     1321 ccagaaacgt agggatgaga gaagcaaggg gaggaattgt tgcttttctc gatgctgacg
     1381 actattgggc cacagatcat ctcgaggaaa gtgtaaatgc tttgttgtta aatggggtgg
     1441 aatttgtttt ttcaaatgta atagaggttg attgttgtgg ttgtgagagt cgaagaaaag
     1501 ttactaatcc ccaatatttg gataataaat acgatataat actgctttct cctcctcaaa
     1561 ctaattcatt cctatttagg aaaaaaatat ttgacactaa agagattttt tttgatgaaa
     1621 acttacgtag acatcaagat tatcaatttc ttactttaat attagaaaaa aatatttcat
     1681 atcaatatat tgataaatat acttcatatt atgtgcaatc acatcgtcct catactgaaa
     1741 gaatggatta taaatctgtt tttttatttt gggaaaaata taattcttat gtttctggga
     1801 gattactaaa aaaatttatt accggtttag ttcttgaggt ggttcttgtg tatgggaaaa
     1861 ataaaacaaa gagttttatt gattcgcatg aacttgtcaa gataaacgca tcgaaactat
     1921 ttctttatct tgtgttaaaa acaaattttg gtgttaatgc taatagggtg atttatcctt
     1981 ttttttatta tattttattc agccccaaaa gcatattaaa aaagatatca caaaggatta
     2041 atagaaagtt ggtacataat gcgtaataag atatctatag ttactacaaa tattactaat
     2101 aatggtggta ctgagcgagt aatagcaaat gttgctaatt ctctttcaga aatttatgat
     2161 attcgtattt atagcctatc tactactgaa ggcgaatgtt tttatcccct aaacgaacgt
     2221 atacatataa aacatatggg gatgatgtgt tatgagcatg aaaaatcagt tttaaaaaaa
     2281 acattcaaaa agttaataaa tgttttctat tcaataaatg aatttaaaaa aataaggtca
     2341 aattatataa ttggagtcaa caagaacata aatatacttc tagttctatc gtttttgtta
     2401 aattataaaa gaaagagaga ttataaactg attgggtggg aacattttgc acataatgct
     2461 cctatgtcac tatttaccag aattttacgt gagctattat ataaatactt agatgattta
     2521 attgttctaa ctaaatttga tgaggagtat tataaagcta agaaaataaa aacaacagtg
     2581 attgaaaatg cgtatacagc aatggatgta gataatctca agactgttag taaaggaaat
     2641 gttattctat caattggaag acatacgtct caaaagcggt ttgataagtt gttacatatc
     2701 tggaaagaaa tagttaaaaa gaatgatggg ttaaaactgc ggattgttgg ggatggtcct
     2761 ctattggatg agaacaaaaa attagcccaa aaattgaatc tgtccgactc agtagtgttt
     2821 gctccgccaa ctaaaaaaat taatgaggaa tatcaaaatg catctttatt tttaatgaca
     2881 tctgactatg aagcatttcc aatggttttg ttggaagctt tgaaaaatgg tcttccttgc
     2941 attgcgtttg actgtgatac tggacctaga gatattatcg ttaatggagt tgatggattt
     3001 gttataccaa taaatgatag tttgaaattt gtagaaaaaa cgatagatgt catgaataat
     3061 gaaaacttaa gataccaact aagctcgaat gcaaaggtta atgtcgaaag gtttaatgaa
     3121 gaacatattt caaaaaaatg gttgattaag ttaaaacaat tgcaataaca tcgacgttaa
     3181 aatcaaggta taagatgatt ataataacat cattcatggt tctgttgttt ttatttacag
     3241 ataatttaaa attaaaaaac tttagaataa tattgtttta ttttttttct ctttgtttca
     3301 gtgtgttagt ttattttaga cctgaataca tgttgaatac tgattacaaa gaatattata
     3361 actattttaa atggattaat tttgataata tttataactt atcggacaca attggtttcg
     3421 aatacggctt cggctatata gtcacgttaa taaaatattt ttttgaatct gaacgtgctt
     3481 gtttttcagg tattgctttt ttatcggtgt ttattagtta taaagctata ttaagattaa
     3541 accccaaaac gaccgcattt tctttttcat tatttttatt atcattttgt gtttatattt
     3601 ttttaggcca aataagacaa ggcatatcac tttcgttagg cttactggct atatcttatt
     3661 tgttggagga taagcgaaaa cagtttttat taaccgttat catagcttca agcattcatg
     3721 tgacggccct aatactaata ttagctccat tcgttaaata ttttaggata aagtatattt
     3781 tttattcctt actactatcg tttgcttttg tttttataga tgtaataaaa ccactgatta
     3841 taaccctagc gcagtttatt ccgtttggta gttttataac tggaaaaata atagcttatg
     3901 gaaatagcga attttcaacc aaagtgggtt tttcttttat tcaagtgtat tatgttttac
     3961 tttctacagt tttatatatc ctagtaaaaa aatatgaata taaaaataat tatatcctca
     4021 cattatgtaa gatttttatg gtcggagtga ttcttaattt tacatttaat tcattttctg
     4081 ttttacttag acttacttat tactatttag cattagattg cattttgatg ggctatctct
     4141 tgaatatggc aaaaaatgga tatacgaaaa tattaatata ttgcccaaca ctaatgctat
     4201 ttatattaag gttttatatg caatggagcg aacatatcaa tggttgaaca ttgtagtaaa
     4261 aataaaaaaa tactcgttga ttgtagaatg attaatgcct ctggaatagg tgtttatact
     4321 cgtgaaatta tcaaagagtt aaaaaaatat agatacttgc agatcgagtt attgcttcaa
     4381 gaagaacaac agttaccaat tgatgttgaa gatattggat ttaaaatcca ctatataaat
     4441 ttttctcgct atagtattaa aaacatcggt ggtatgaagc atttactgaa tggctttgat
     4501 gtctatttta tgccatcatt atcgatacca cctctattaa aaaaaggctg tttgataatc
     4561 acaacagtac atgatctgtg tcctgttgca ttacggaaat tatttggatc caaaaaagct
     4621 gtagcttatt gggctttgct attaatacaa cttgtttgtt ctaataagat aatttccatt
     4681 tcaagattca ctgctaaaga aataaataag tattactttg gtttatttaa aagtaaatta
     4741 tatgtcattg gcaatggtat aagaaatatt tttaatttgg aattaaatag tgaaacagaa
     4801 aattcccatg aagaatatgg tatttgcgtg ggcaatatta agcctcataa aaatgttgta
     4861 ccactaattg attaccttaa gacaaacatc attaacaaaa aaatatattt tataggtgaa
     4921 ataaatgggt tttcaacgaa aataaataaa agtttgctta acgatttgcc tgaaaatata
     4981 gtttttacag ggagagtaag tgatcaagaa ttacataatt attaccgtaa tgccagtttt
     5041 tttatattcc cctcattata cgaaggcttt ggtttgcctt tgctagaggc tatgaattat
     5101 aggttgccta tttttgcatc aaatattgat gtatttaagg aagttgctgg tgaaacaatt
     5161 aattattttg atccatatac atttgatgct cttgcagata gaattaacaa attaaaaaag
     5221 aacaagaaat ctattgaatc ttatgcacga gttttgcagt tatatagttg ggataataat
     5281 gttgcaagtc tattaaggat tatgaatgaa gaagatattg ctcgtaaata aatactatca
     5341 tccagacatt ggtggcgtag aaactgttgt acaacagcat gctcaattat tgaagtatga
     5401 atatagtgta acagtgttgt gtattagcaa agaattctca tggtatacaa aaaatgaatt
     5461 cattaatgga gttaaagtaa tacgctgttc tccattggga acttattttt ctatgccgtt
     5521 atcaattact ttttttttgc attacttcat taattattta aaatcagacg tgattattaa
     5581 tcacattccg tttccattaa tggatttagc attttttatc attgatagat tagtcaaaag
     5641 aaaaaatata ttattttggc acagtgatat agtaaagcaa aaagcattaa aaaaaatatt
     5701 acgtccgttc attataaata ctattaatcg aagcactcag attttaacta catctccaaa
     5761 tctcaaagct aattcagaag acatcggtga atatcagaaa aaaacagatg tgttaccatt
     5821 atttattaat tcaaatgtta taaatggtta tattaaaaat ttaaacgcag aaaaaaaata
     5881 tgactttata ttttttggaa gattatgtta ttataaagga gttgatattc ttttagatgc
     5941 agtaaaaatt ttgaaaaata aaggagctga acctgaaatt tttatggcag gtgatggtga
     6001 tttagttaat tatatcgaaa atgaaattat tgaaaataaa attaaaaata taacttttat
     6061 taaacggttt ttaacagagg aggaaaaata tcattatctt tcaatttcaa aatgttttct
     6121 tttcccatcc gttgctgcat ccgaagcatt tggtatcact cagcttgagg caatgtctct
     6181 tgggctccct gtaataaaca caaaccttaa tactggcgtt ccttttgtca gtttaaatgg
     6241 agttacgggg ataacagtaa ctgcaggtaa tcctgatgat ttagctaatg caatgctgtc
     6301 tttgttgcgc gatgatgaaa aatatgaatc gttcaagaca aattgtaaag ttagggtttc
     6361 tgagttgttt gaggaaaatg ttgtgaaaaa taaactattc aaagtaattg aagaattaaa
     6421 ctgagaagtt gaattttcac tcatactata ttcattaatt atacgtattt atatacttaa
     6481 cttatgtcaa ataatattat ctgtcctgtg attatggctg gtggttctgg tagccgtcta
     6541 tggcctcttt ctcgtctttt ttatccaaag caatttctaa aattagtaga tgattcaagt
     6601 atgctacaag aaacaattag tcgccttgga gatcttcctt gtagcttacc tgttgttata
     6661 tgtaatgaac agcatagatt tattgttgct gaacagcttc gaatgattaa taagttaacg
     6721 aataacatta tattagaacc tgtagggcgc aatacagccc cagcaatagc tttagcagca
     6781 atgatctctg agtctttttc ttctttagaa gatcccttac ttttagtatt agctgctgat
     6841 catgatatta aaaatcataa atcatttcgt gatgctgtaa ggaaagctat tccttttgca
     6901 gaacaaggta aattagttac ttttggtatt gttccaagta tggctgaaac aggatatggc
     6961 tatattaagc gtggacatga aatttctttg gaaaatgatg aagtcgcgta tactgtttcg
     7021 aagttttatg aaaaacctaa tataagtgat gcccaaaaat tcctcgcttc tggaaaattt
     7081 tattggaata gtggtatgtt tttatttaga gcttctcgtt atttgagtga gttaaataaa
     7141 tttagaccag atatatactt agcatgcgca tcttctacta aaaatataaa taccgatctt
     7201 gattttattc gtgttgatga aaattcattt tctaactgcc cagatgaatc catagactat
     7261 gcagtcatgg aacaaacttc tgatgcagtg gttgttccta tgaatgcagg ttggagtgat
     7321 gttggatcat ggtcttccct atgggagatt agtaaaaaaa atgaacaagg gaacgttagt
     7381 catggtgatg ttattaatca caatactgag aatagttatg tctatgccga gtctggtttg
     7441 gtgactacca ttggggtaaa agacttggtt gttgtacaaa ccaaagatgc cgttcttgtt
     7501 gcagatcgca attctgtgca ggatgtgaaa aaagtagtgg agcagattaa agcccatggt
     7561 cgttccgaac atcagatgca tagagaagtt tatcgtccat ggggtaaata tgactctatc
     7621 gacgcaggcg agcgctacca ggtgaaacgc ataaccgtga aaccgggcga ggggttgtca
     7681 gtacagatgc attaccaccg ttcggaacac tgggtagtgg tagcgggaac ggcaaaagtc
     7741 actattgatg gggttatcaa acttcttggc gaaaacgagt ccctctatat tccgcaaggg
     7801 gcgaagcatt gcctggaaaa tccggggaaa ataccgcttg agttaataga agttcgctcc
     7861 gggtcttatc tcgaagagga tgatgtggtg cgttttgcag atcgttatgg aagagtctag
     7921 ttttgtacaa ctttcatttt ccctaatgat tctcgacctg aaataattct gccctgttca
     7981 tgtaaattat ttgaatacca ctctaatttg agtgttgaaa atgagtgttt aaaatgaaaa
     8041 aattaaactg ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag
     8101 atattgcctg gcgtattggg cgtgcctatg gcgaatttct caaaccgaaa accattgtat
     8161 taggcggtga tgtccgcctc accagcgaaa ccttaaaact ggcgctggca aaaggtttac
     8221 aggatgcggg cgtcgatgtg ctggatattg gtatgtccgg caccgaagag atctatttcg
     8281 ccacgttcca tctcggcgtg gatggcggca ttgaagttac tgccagccat aatccgatgg
     8341 attacaacgg catgaaactg gtgcgcgaag gggctcgtcc gatcagcggt gataccggac
     8401 tgcgcgatgt ccagcgcctg gcagaagcca acgacttccc tcccgtcgat gaaacaaaac
     8461 gcggtcgcta tcagcaaatc aatctgcgtg acgcttacgt tgatcatctg ttcagttata
     8521 tcaacgccaa aaacctcacg ccgctcaagc tggtgatcaa ctccgggaac ggcgcagcgg
     8581 gtccggtggt ggacgctatc gaagcccgct ttaaagccct cggcgcacct gtggaattaa
     8641 tcaaagtgca caacacgccg gacggcaatt tccccaacgg tattcctaac ccgctgctgc
     8701 cggaatgccg tgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat atgggcattg
     8761 cctttgacgg cgattttgac cgctgtttcc tgtttgacga aaaagggcag tttatcgagg
     8821 gctactacat tgtcggtctg ctggcagaag cgttcctcga aaaaaatccc ggcgcgaaga
     8881 tcatccacga tccacgtctc tcctggaaca ccgttgatgt ggtgactgcc gcaggcggca
     8941 ccccggtaat gtcgaaaacc ggacacgcct ttattaaaga acgtatgcgc aaggaagacg
     9001 ccatctacgg tggcgaaatg agcgcccacc attacttccg tgatttcgct tactgcgaca
     9061 gcggcatgat cccgtggctg ctggtcgccg aactggtgtg cctgaaagga aaaacgctgg
     9121 gcgaactggt gcgcgaccgg atggcggcgt ttccggcaag cggtgagatc aacagcaaac
     9181 tggcacaacc tgttgaggcg attaaccgcg tcgaacagca ttttagccgt gaggcgctgg
     9241 cggtggatcg caccgatggc atcagcatga cctttgccga ctggcgcttt aacctgcgct
     9301 cctccaacac cgaaccggtg gtgcggttga atgttgaatc aaaagccaac gttgagttac
     9361 tcgaaagcag ggtttctata atattaaata ttctaaatat gtgactaatt taattgtgtg
     9421 cttaaaatta ggagttcaat gtgaattata tttctttaga aaccttgatg aatgatatta
     9481 gagactcaat aaatctattg agtaaacata attttgattt agttgtaggc attccaagaa
     9541 gtggtatgat accagcctat ctgatcagtc tttatttgaa tattgacgta accgatgtta
     9601 attcttttat cctaaataca ccaattcaaa gaggatcgac tagaacatca ggaaaacgca
     9661 tatataaccc acatgatgca caaaggattc tcttagtcga tgatagcttt tcaacaggta
     9721 aatcgatgag gaatatttta gattctatac cagtggattt aaaaaaaaat attaaaacaa
     9781 tggttgccta tacatctgat gtaaatggcg ctggattaga tatttatata cgtcatgttt
     9841 ctcaccccag attgtttgaa tggaatatac tcaatcacag catcattagc aatagctgtt
     9901 tcgatattga tggtgtttta tgtgtcgatc ctaatgagct tcagaatgac gatggtgaaa
     9961 actatataaa atttatatgc gaggcagaac ctaagtttat acctaagttt aagataaaat
    10021 acttggttac aaaccgtctt gaaaaataca gagacataac tgaagcatgg ttgttaaaaa
    10081 ataacataca atatgagaaa ttaatcatgt taaatatggc gacaaaagaa gaaaggcaat
    10141 ctgcaggaat tcattccaga cataaagccg aattttataa acgttccggt tgtgatttat
    10201 ttgttgaaag tgatattaat caggcgattg aaattatgaa atataccggg aagtatgtat
    10261 attgtataga taataataga atgtactctc ctagcgccat acaatattta tctaaaaggc
    10321 cgcttgactt tatgaatgat ataattcttt atttgccacg aattttatat cgtagattac
    10381 ccttagaaac aaagatggct ataaaaaaaa ggttaaaacc ttaaaaagca tttcaacctt
    10441 tttcaattta cttattttta tttaattaac cgattaatga agtttttgac ttctttagtc
    10501 ggtaataatg aaactataaa cacgacgaaa catataatag tcaaaataac tagtgataag
    10561 aaactatggt ccaagtgttt ttttaatata tatattatat aatagctaat gaagcctgct
    10621 ataaataaag gataagtttt caaaataagt ttgtaaaagt ttgattttat cacatacatg
    10681 gttaaagcca tacatggaaa aaaatgaact aaattagcta tgaaataata ttttgcgagt
    10741 gaaatgatat tgtcattaac gccaatcaca aatgcaatac ctgtcaagat ggctccttgt
    10801 accccaagta agacaaggat accagtacgg ccttgggaca taaatattat acctgtggtg
    10861 ctcaaaatgc tttgaatgat agctgtaggc gctaaccata caagaattga tgaagataaa
    10921 tgccattttt ctccaaaaat tatatttatt accatttcat ttaaaccaag tagagatata
    10981 ataagaggaa tggttataaa ccaaatcgta tatactgtat ttaaatatat tttttctacc
    11041 tcagcatgct tgtgtttatt tttacttaat atagggaata acgaacggtt tacaataaac
    11101 gttattgaag taagtgggaa aagcataatt ctatatgcta gattatatgc acctaatata
    11161 gttgctgaca tgtacttacc tatgaggaaa ctatctaaat tacgagcaaa gtaattaatg
    11221 aaattaaaaa gagatagctg atagctgaaa gaaaatattg atttggtatt tttaaatata
    11281 ttctcaattt tgattctagg actccatgaa cttaaatacc aaaacaaacc tgaagataac
    11341 aacgtgctaa caatagattg agtgactaaa ctatataccc caaagttgag atatgccatt
    11401 gctatagcaa tgattaaagc agagccaaaa gaaaaaattt ctatttttga gatggtttca
    11461 aattttgact ctctttcaag aagtgataag tgaacagatg ttgccccaag taagaaaaaa
    11521 ttaatagata gcaatgataa aacaggtttt aatttaggta cgtcgtataa ttcagaaaaa
    11581 taagatgagc taaataaaat tgcagttcct actattatgc ctaataataa atttagccaa
    11641 aagatagcag atttgagact ttcgtcgatc gaatctctct ggactatagc agctgatgtt
    11701 cctaagtcac gaaaaaggat tgcgaaattg gttaccacgg tggccatagc cataatacca
    11761 taatcttctg gtgcaattaa tctggcaaga taaatcatac ttataagctg tgttgctatt
    11821 ttaaataatt gtgatatagc gttccacttt aagttgttaa aaatgctcat tattagttct
    11881 ctagagtgac ctataaaaag taaaaataaa aatccaacac ccttagtatt cttacataaa
    11941 ataaaaaact agaaaagcta attatactga aaggtaacat gagttatagt ttattatttt
    12001 ttaaaataag taaacttgaa tctcgcatac atcgcgatga acatcccctg acaggagaaa
    12061 acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag taatggggcg caaccttgcg
    12121 ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgctcccg tgaaaagacg
    12181 gaagaagtga ttgccgagaa tccaggcaag aaactggttc cttactatac ggtgaaagag
    12241 tttgttgaat ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc
    12301 acggatgctg ctattgattc cctcaaacca tatctcgata aaggcgacat catcattgat
    12361 ggtggtaaca ccttcttcca ggacaccatt cgtcgtaatc gtgagctttc tgccgaaggc
    12421 tttaacttca ttggtaccgg tgtttccggt ggtgaagaag gtgcgctgaa aggtccttcc
    12481 attatgcctg gtgggcagaa agaagcctat gaactggttg caccgatcct caccaaaatc
    12541 gccgcagtgg ctgaagacgg tgagccatgc gttacctata ttggtgccga tggcgcgggt
    12601 cactatgtaa aaatggttca caacggtatt gaatacggtg atatgcagct gattgctgaa
    12661 gcctactctt tgcttaaagg tggcttgaac ctttccaacg aagaactggc gcagaccttt
    12721 accgagtgga ataacggtga actgagcagc tacctgatcg acatcaccaa agatatcttc
    12781 accaaaaaag atgaagacgg taactacctg gttgatgtga tcctggatga agcggctaac
    12841 aaaggtaccg gtaaatggac cagccagagc gcactggatc tcggcgaacc gctgtcgctg
    12901 attaccgagt ctgtgtttgc acgttacatc tcttctctga aagatcagcg tgttgccgca
    12961 tctaaagttc tctctggccc gcaagcacag ccagcaggcg acaaggctga gttcatcgaa
    13021 aaagttcgtc gtgcgctgta tctgggcaaa atcgtttctt acgctcaggg cttctctcag
    13081 ctgcgtgctg cgtctgaaga gtacaactgg gatctgaact acggcgaaat cgcgaagatt
    13141 ttccgtgctg gttgcatcat ccgtgcgcag ttcctgcaga aaatcaccga tgcatacgcc
    13201 gaaaatccgc agatcgctaa cctgctgctg gctccgtact tcaagcaaat tgccgatgac
    13261 taccagcagg cgctgcgcga tgtcgtcgct tatgcggtac agaacggtat cccggttccg
    13321 accttcgccg ctgcggttgc ctattatgac agctaccgcg ccgcagttct gcctgcgaac
    13381 ctaatccagg ctcagcgcga cta
//
