LOCUS       AY529126               13367 bp    DNA     linear   BCT 04-MAY-2004
DEFINITION  Shigella boydii serotype 11 O-antigen gene cluster, complete
            sequence.
ACCESSION   AY529126
VERSION     AY529126.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Tao,J., Feng,L., Guo,H., Li,Y. and Wang,L.
  TITLE     The O-antigen gene cluster of Shigella boydii O11 and functional
            identification of its wzy gene
  JOURNAL   FEMS Microbiol. Lett. 234 (1), 125-132 (2004)
   PUBMED   15109730
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   Tao,J., Feng,L., Guo,H. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2004) College of Life Sciences, Nankai
            University, Tianjin 300071, P. R. China
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="11"
                     /db_xref="taxon:621"
     gene            1111..2196
                     /gene="rmlB"
     CDS             1111..2196
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="AAS98026.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGYLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIARELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2196..3095
                     /gene="rmlD"
     CDS             2196..3095
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="AAS98027.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKTEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLVLNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3153..4031
                     /gene="rmlA"
     CDS             3153..4031
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="AAS98028.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKVLAEPL
                     KKNAYGQYLLKMIEGY"
     gene            4036..4569
                     /gene="rmlC"
     CDS             4036..4569
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="AAS98029.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDSFINIQWPYA
                     ECIILSDKDKHQPRLSR"
     gene            4667..5410
                     /gene="wbsV"
     CDS             4667..5410
                     /gene="wbsV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS98030.1"
                     /translation="MCKIVVYTAITGNYDNIKPLSYVNTNFDYLCFTDYEYTGVIPEP
                     WKQIRMPPAKWCNKDLARYIKMNVHEILPNYEASVWIDGNIDIINNIEGLVFDALKKG
                     GASSYQHWGRNNINEEMIECAKIGYDSIFILLKQMKQYGNEGFISNELYETNVLIRDH
                     TNSSISEFSKIWWEQYMQYGKRDQYAFTYAAWKSGLNIHNLGRHDPRFIKKYFSYFPH
                     KNGKSIKVYRIKQLINKITNRITLRIIKI"
     gene            5432..6460
                     /gene="wzy"
     CDS             5432..6460
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAS98031.1"
                     /translation="MVYGFLWCLCFLLSLFNNNSYFKYYITLCIIMVLWFLASFRGAG
                     VDGDYANYIEYINDAQSIGYSYRGGYFFDWIVNFFVYIGLPATYVFAVYALAIPIKIK
                     LFQKFSLLSSSILLGYVGFFIYLHDFTQIRAGLAVGIAFWGIYYYAYQNKIKAVILFF
                     ASCFIHPSLIFLVAFVCFNKFLSNKLLVVTLMISIILCIINANTFIVQKIVDLSGSAD
                     LDLYYRLAVDGQIIKTFGAFPLLNLFISIVSLYYLNNIARENALLSIFIKMLLFSQIS
                     WFLFSPIPVLAARISQIFLFSIVFVLPHLSVKLFNQPITIPALYSFVGFLAFIIIAEL
                     MHPYQLGF"
     gene            6468..7310
                     /gene="wbsW"
     CDS             6468..7310
                     /gene="wbsW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS98032.1"
                     /translation="MKITILVVLYNKKFKESPTLQTILSEKHTLKKMNVELCIHDNSP
                     NSQLDECYISEMKEFVKCYYTHTPENMTLREIYNNIINTLKYNTYLCLMDDDTSLPQS
                     FFNEVISAINKNPDIELIVPRVIVNDKLYSPHKSYSFINRPTFRDIKGIQKSRNMSAI
                     NSGMVIKSNFFKRCGFKYPEYTSYYGTDKAFFDHYLKYNQEYYVLDIDINHDVSNHPH
                     NKDDVRYSQIISSVNFFWMQHLKGNYALKFLYIIYIILYRIKLSILRKNLIFIKSIVS
                     LGNK"
     gene            7310..8446
                     /gene="wbsX"
     CDS             7310..8446
                     /gene="wbsX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS98033.1"
                     /translation="MKNNSYKVFPFYFPQFYATKENDMWWGKGFTDWELVKKAQAVHQ
                     SQSQPRIPLNGYYDQSDPVVIKKQSKLASEYGIDGFNFYHYWFDGTVLLDKPMQNLYN
                     DKSIDIEYFFTWANETWTRQWVGRPQDLLIKQEHKVDINIWNEHYEYLRKFFLDDRYL
                     KINNSPVLCIYRPELLKSLREWVAFFNNKAKCDGFDGIHLIALRAYSIANADSVYKYF
                     DKIVNFQPRFAINTHLKKSSPLKKLIESTLRIAPEWLQLQLVRIIKNKRATYNHYKYS
                     DYIQSMKNDVTEYKGKPIYPVVFPDWDNAPRYKENATFFCESSAFDFEKALNIACDIT
                     RNHDDKLIFINAWNEWSEGAYLEPDEMYKYSNLEIIKKVFQDKR"
     gene            8443..9327
                     /gene="wbsY"
     CDS             8443..9327
                     /gene="wbsY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS98034.1"
                     /translation="MKRIAILLAAYNGARWLEEQISSILQQTNVEVTIYVSIDLSNDE
                     TYLLVSKLALKEPRIIILPYGDIYGGAAKNFYRLICEVDYENYDYIALSDQDDVWRKN
                     KLERACEFLKNNDFYSSNVIAFWENGKQALITKSQDKVAFDHFFESAGPGCTFVFKRE
                     YALQLKRFLMINKHAMDVELHDWLIYAFARENNLKWHIDSYPGMYYRQHKNNQVGANN
                     SLKAIIKRIRLIKSGWYRNEIIKILNLFEQSDIPFRDNLCNKNYFTSLFLLKYIYMIR
                     RKRKDRAFLVLMIIFGLF"
     gene            9344..10327
                     /gene="wbsZ"
     CDS             9344..10327
                     /gene="wbsZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAS98035.1"
                     /translation="MNFVSLENMISDIRGFIPELAIHDFDLIVGIPRSGMIPAYTIGL
                     YLNIDVTDVSSFVKNVPLQRGISREKPTSLKLPQDAKKILLVDDSYTTGKSLANTLGK
                     IPMELRNRIVSFALYTTDRENHNLDMFIRVVKYPRVFEWNILNHNIIGNSCLDIDGVL
                     CMDPTDDDNDDGQKYITFLENAKPKFIPKVKIKYLVTNRLEKYRTHTEKWLSKNNVKY
                     EQLIMLDLKTKEERIRSGLHSTHKAKFYKHSGCSLFIESDVKQAYEIMMQSGLPVYCI
                     DDNTMYKPGMTKYFIKQPSNALKKIILWLPILIYKNIPKPYQKIIRRLIKK"
     gene            complement(10367..11794)
                     /gene="wzx"
     CDS             complement(10367..11794)
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAS98036.1"
                     /translation="MSIFNNLKWNTFSQVFKILTQLISIVYLARIIPPNEYGIMAMAG
                     VVMNFASLFRDLGTSAAIIQKEQLQDETKSAVFWLNFLLGFLVFIIITTLTPFISHLY
                     NQPKLLIVLPLLSFSFIISGSSSLHLAMLERESKFFIISKIEVFSAALSLLLAIILAN
                     NSFGIYSLVFQSLLNSLSSAVLLWIVSPWRPKGKPRHVINHLKEIFRFTSQLSLFNII
                     NFFARNLDSFLIGKYMSASILGSYNLAYRIMLFPLSSLTFIANRSLYPILSQKKNNYK
                     EINDIYLNTIYIIWFITLPLVTVITSLNKLFIQVVFGEQWILSADILVWLAPTAIIQS
                     VLSTTGTIFMSQGKTGILLLLGIQGTILTAVSFMVGIHCGIVDLAKYYFIANAIHFIP
                     CIIFTMKTIKSNILSIFKKTYPLFISAAISFILISNVRVHFASDIKSLFVLSAIGGTI
                     YLLLLLPISESRKLIKTLQTKIKKR"
BASE COUNT     4288 a   2195 c   2728 g   4156 t
ORIGIN
        1 ctcctggtaa ctcacgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgaa
       61 ttagaatctc tccttgaact gcgcgtgaag cgtcaactgc tggccgaagt acagtccatt
      121 tgcccgccgg gcgtgaccat tatgaacgtg cgtcagggcg aacctttagg tttaggccac
      181 tccattttat gtgcacgacc cgccattggt gacaatccat ttgtcgtggt gctgccagac
      241 gttgtgatcg atgacgccag cgccgacccg ctgcgctaca accttgcagc catgattgcg
      301 cgcttcaacg aaacgggccg tagccaggtg ctggcaaaac gtatgccagg tgacctctct
      361 gaatactctg tcatccagac caaagagccg ctggatcgcg aaggcaaagt cagccgcatt
      421 gttgaattta tcgaaaaacc ggatcagccg cagacgctgg actcagacat catggccgta
      481 ggtcgttatg tgctttctgc cgatatttgg ccggaacttg aacgcacgca gccaggggca
      541 tggggacgta ttcagctgac agatgccatc gctgaactgg cgaaaaaaca gtccgttgat
      601 gcaatgctga tgactggtga cagctacgac tgcggtaaaa aaatgggcta tatgcaggcg
      661 tttgtgaagt atggactacg caacctgaaa gaaggggcga agttccgcaa aggtattgag
      721 aagctgttaa gcgaataatg aaaatctgac cggatgtaac ggttgataag aaaattataa
      781 cggcagtgaa gattcgtggc gaaagtaatt tgttgcgaat tttcctgccg ttgttttata
      841 taaacaatca gaataacaac gagttagcaa taggattttt gtcaaagttt tccaggattt
      901 tccttgtttc cagagcggat tggtaagaca attagcgttt gaatttttcg ggtttagcgc
      961 gagtgggtaa cgctcgtcac atcgtaggca tgcatgcagt gctctggtag ctgtaaagcc
     1021 aggggcggta gcgtgcatta atacctctat taatcaaact gagagccgct tatttcacag
     1081 catgctctga agtaatatgg aataaattaa gtgaaaatac ttgttactgg tggcgcagga
     1141 tttattggtt ctgctgtagt tcgtcacatt ataaataata cgcaggatag tgttgttaat
     1201 gtcgataaat taacgtacgc cggaaacctg gaatcacttg ctgatgtttc tgattccgaa
     1261 cgctatgttt ttgaacatgc ggatatttgc gatgcagctg taatggcacg gatttttgct
     1321 cagcatcagc cggatgcagt gatgcacctg gctgctgaaa gccatgtgga ccgttcaatt
     1381 acaggtcctg cggcatttat tgaaaccaat attgttggta catatgtcct tttggaagcc
     1441 gctcgcaatt actggtctgc tcttgatagc gacaagaaaa atagcttccg ttttcatcat
     1501 atttctactg acgaagtcta tggttatttg cctcatcctg acgaggtgaa taatacagaa
     1561 gaattaccct tatttactga gacaacagct tacgcgccaa gcagccctta ttccgcatcc
     1621 aaagcatcca gcgatcattt agtccgcgcg tggaaacgta cctatggttt accgaccatt
     1681 gtgactaatt gttcgaataa ctacggtcct tatcactttc cggaaaaatt gattccacta
     1741 gtaattctta atgctctgga aggtaaggca ttacctattt atggcaaagg ggatcaaatt
     1801 cgcgactggc tgtatgttga agatcatgca cgtgcgttat ataccgtcgt aaccgaaggt
     1861 aaagcgggtg aaacttataa cattggtgga cacaacgaaa agaaaaacat cgatgtagtg
     1921 ctcactattt gtgatttgtt ggatgagatt gtaccgaaag agaaatctta ccgcgagcaa
     1981 attacttatg ttgccgatcg tccgggacac gatcgccgtt atgccattga tgctgagaag
     2041 attgctcgcg aattgggatg gaaaccacag gaaacgtttg agagcgggat tcggaagaca
     2101 gtggaatggt acctgtccaa tacaaaatgg gttgataatg tgaaaagtgg tgcctatcaa
     2161 tcgtggattg aacagaacta tgagggccgc cagtaatgaa tattctcctt ttcggcaaaa
     2221 cagggcaggt aggttgggaa ctacagcgtg ctctggcacc tttgggtaat ttgattgctc
     2281 ttgatgttca ctccactgat tattgtggtg attttagtaa tcctgaaggt gtggctgaaa
     2341 ccgtcaaaaa aattcgccct gatgttattg ttaatgctgc ggctcatacc gcagtagata
     2401 aggctgagtc agaacccgaa tttgcacaat tactcaatgc gaccagcgtt gaatcaattg
     2461 caaaagcggc taatgaagtt ggggcctggg taattcatta ctcaactgac tacgtatttc
     2521 cgggaaccgg tgaaatacca tggctggaga cggatgcaac cgcaccgcta aatgtttacg
     2581 gtgaaaccaa gttagccgga gaaaaagcgt tacaggaaca ttgcgcgaag catcttattt
     2641 tccgtaccag ctgggtatac gcaggtaaag gaaataactt cgccaaaacg atgttgcgtc
     2701 tggcaaaaga gcgtgaagaa ttagcggtta ttaacgatca gtttggtgca ccaacgggtg
     2761 ctgagctgct ggctgattgt acggcacatg ctattcgtgt tgcactgaaa aaaacagaag
     2821 tcgcaggctt gtaccatttg gtagctagtg gtaccacaac ctggtacgat tatgctgcgc
     2881 tggtttttga agaggcgcgc aaagcaggca ttccccttgt actcaacaag ctcaacgcag
     2941 taccaacaac tgcctatcct acaccagctc gtcgtccaca taactctcgc cttaatacag
     3001 aaaaatttca gcagaacttt gcgcttgtct tgcctgactg gcaggttggc gtgaaacgaa
     3061 tgctcaacga attatttacg actacagcaa tttaatagtt tttgcatctt gttcgtgatg
     3121 gtggagcaag atgaattaaa aggaatgatg aaatgaaaac gcgtaaaggt attattttag
     3181 cgggtggttc tggtacacgt ctttatcctg tgacgatggc cgtcagtaaa cagctgttac
     3241 cgatttatga taaaccgatg atctattacc cgctttctac actgatgtta gcgggtattc
     3301 gcgatattct gattatcagt acgccacagg atactcctcg ttttcaacaa ctgctgggtg
     3361 acgggagcca gtgggggcta aatcttcagt acaaagtaca accgagtccg gatggtcttg
     3421 cgcaggcgtt tattatcggt gaagagttta ttggtggtga tgattgtgct ttggttcttg
     3481 gtgacaatat cttctacggt cacgatctgc cgaagttaat ggacgtagct gttaacaaag
     3541 aaagtggtgc aacggtattt gcctatcacg ttaatgatcc tgaacgctac ggtgtcgttg
     3601 agtttgataa aaacggtacg gcgatcagcc tggaagaaaa accgctacaa ccaaaaagta
     3661 attatgcggt aaccgggctt tatttttatg ataacgacgt tgtcgaaatg gcgaaaaatc
     3721 ttaagccttc tgcccgcggt gaactggaaa ttaccgatat taaccgtatt tatatggaac
     3781 aggggcgttt atccgttgcc atgatggggc gtggatatgc atggctggac acgggaacac
     3841 atcaaagtct tattgaagca agcaacttca ttgcaacaat tgaagaacgt caggggctga
     3901 aagtatcctg cccggaagaa attgcttacc gtaaaggatt tattgattct gagcaggtga
     3961 aagtattagc tgaaccgctg aaaaaaaatg cttatggtca gtacctacta aaaatgattg
     4021 aaggttatta ataaaatgaa cgtaattaaa actgaaattc ctgatgtgtt aatttttgag
     4081 ccgaaagttt ttggtgatga gcgtggtttc tttatggaaa gctttaatca gaaagttttc
     4141 gaagaggctg taggacgtaa ggttgaattt gttcaggata accattcgaa gtctagtaaa
     4201 ggtgttttac gcgggctgca ttatcagtta gaaccttatg cgcaagggaa attggtacgt
     4261 tgcgttgttg gtgaggtttt tgatgtagct gttgatattc gtaaatcgtc gcctaccttt
     4321 ggtaaatggg ttggggtgaa tttatctgct gagaataagc ggcaattgtg gatacctgaa
     4381 ggatttgcac atggtttttt ggtgctgagt gaaacggcgg agtttttgta taagacgact
     4441 aattattatc atcctgaaag tgatagggga attatttggg atgattcctt cattaatatc
     4501 caatggcctt atgctgagtg cattatttta tctgacaaag ataaacatca gcctagatta
     4561 tctagataat taatataacc taggtttttt attgttattc atgagctatc tttatgaatg
     4621 gttgagtttc ccaatttaaa acagattaat ttgtcaaggt caaaatatgt gtaaaattgt
     4681 ggtgtataca gctattacag ggaattatga taatattaag cccctgtcat acgttaatac
     4741 caactttgat tatttgtgtt ttacagacta tgaatataca ggtgttattc ctgagccttg
     4801 gaaacagatt cgaatgccac cagcgaagtg gtgtaataag gacttggcac gatatattaa
     4861 aatgaatgtg catgagattt tacctaatta tgaagctagt gtttggattg atggaaatat
     4921 agacattatc aacaatattg agggtttggt ttttgatgcc ttaaaaaaag ggggggcctc
     4981 atcataccaa cattggggcc gaaataatat taatgaggaa atgattgagt gtgcaaaaat
     5041 tggatacgat agtattttta ttcttttgaa acaaatgaaa caatatggta atgaaggttt
     5101 catctcaaac gaactgtatg aaacaaatgt tttgattaga gatcatacta atagcagtat
     5161 ttctgaattc tctaaaattt ggtgggagca atatatgcag tatggtaaac gagatcaata
     5221 cgcctttact tatgctgcgt ggaaatctgg actaaatatt cataatttag gacgacatga
     5281 ccctagattt attaaaaaat atttttcata ttttccacat aaaaatggga aaagcataaa
     5341 agtatatagg ataaaacaat taattaataa aataaccaat cgtattacgc tcagaattat
     5401 taaaatataa cagtatgaag gtttatttat aatggtttac ggttttctat ggtgtctatg
     5461 ttttctatta agtttgttta ataataacag ttattttaaa tattatatta cactatgtat
     5521 aataatggtt ctttggtttt tggcttcctt taggggggca ggagttgatg gtgattatgc
     5581 aaattatatt gagtacataa atgatgctca aagtattgga tattcttata gaggtggtta
     5641 tttttttgat tggatagtta atttttttgt ttatattggc ttgcctgcca catatgtttt
     5701 tgcagtatat gcacttgcca taccgataaa aataaaatta tttcaaaagt tttcattatt
     5761 atcttcttct atattactcg gttatgttgg tttttttatt tatctgcatg attttactca
     5821 aattagagct ggcttagctg ttggtattgc tttttggggg atttattatt acgcatatca
     5881 gaataaaata aaggcagtaa tactattttt tgcgagttgc tttattcatc catcgctcat
     5941 atttcttgtg gcctttgtat gctttaataa attcttgtct aataaattat tagtggtcac
     6001 actgatgata agtattattt tatgtataat aaatgcaaat acatttatag tacagaaaat
     6061 agttgattta agtggcagtg cagatcttga tttatattat cgcttggcag ttgatggtca
     6121 gattattaaa acatttggag catttccatt actgaattta tttatatcga tagtatcact
     6181 gtattatttg aataatatag caagagaaaa tgctttactt tcaatcttta ttaagatgct
     6241 tttgttttcg caaatttcat ggttcctttt ctctcccata cctgtattag ctgcacgaat
     6301 aagtcagatt tttttattct caattgtctt tgttttgccg catttatctg taaagctatt
     6361 taatcagcca ataactatac cagcactata tagctttgtg ggttttttag cctttattat
     6421 tatagccgaa ttaatgcatc catatcagtt aggattttga tacataaatg aagataacta
     6481 tcttggtagt gctttataat aaaaaattta aagaaagccc aacattacag acaatattga
     6541 gtgaaaaaca taccttgaaa aaaatgaatg ttgaactctg tattcatgac aattcaccca
     6601 attcgcaatt agatgagtgt tatattagtg agatgaagga atttgtaaaa tgttattata
     6661 cacacacacc agaaaatatg acattaagag agatatataa taatataatt aataccttaa
     6721 aatacaatac ttatctttgt ttgatggatg atgatactag tttaccacag tccttcttta
     6781 atgaggtaat aagtgctatc aataaaaatc cagatattga gttaattgta ccacgagtta
     6841 tcgttaatga taagctttat agtccacata aaagttattc atttataaat agaccaacat
     6901 ttagggatat taaaggaata cagaagtcca ggaacatgtc ggctataaat agtgggatgg
     6961 taatcaaatc aaattttttt aaacgttgtg ggtttaaata tcctgaatat acaagttatt
     7021 atgggacgga taaggctttt tttgatcatt atctaaaata taatcaagaa tattatgttt
     7081 tagatattga tataaatcat gatgtaagta accatccaca taataaagat gatgtacgtt
     7141 attcacagat tatttcatct gtgaatttct tttggatgca acatttaaaa ggaaactatg
     7201 cgcttaagtt tttatacata atttatatta tattatatcg tatcaaacta tccatactca
     7261 ggaaaaattt aatttttatt aaatcaatag tatctctagg taataaataa tgaaaaataa
     7321 tagctataaa gtatttcctt tttattttcc tcaattttat gcaacgaaag aaaatgatat
     7381 gtggtggggg aaaggtttta ctgattggga actggtaaaa aaagctcaag cagtacatca
     7441 atctcagtca caacctcgta ttcctctaaa tggctattat gatcagtctg atccagtagt
     7501 aataaaaaaa caatcaaaat tagcaagtga atatggaata gatggtttta atttctatca
     7561 ctactggttt gatggtactg tgttgctaga taaacctatg caaaatttat ataatgataa
     7621 aagtatcgat atagaatatt tctttacatg ggctaatgaa acatggacaa ggcagtgggt
     7681 tggacgcccg caagatctac taataaagca agaacataaa gtggatataa atatatggaa
     7741 tgaacactat gaatatttga ggaaattttt tcttgacgat aggtatttaa aaatcaataa
     7801 ttcacctgtg ctttgcatat atagacctga acttttaaaa tcgttaagag aatgggtggc
     7861 tttttttaat aataaagcaa agtgtgatgg atttgatggc atacatttga tcgcattacg
     7921 agcatacagt attgcaaatg cagattctgt ttataaatat tttgataaaa ttgttaactt
     7981 tcaacctaga tttgcaataa acactcattt aaaaaaatcg agtccactaa agaaattaat
     8041 tgaaagcact ctaaggattg ctccagagtg gcttcagttg cagcttgtaa gaataataaa
     8101 aaataagaga gcaacttata atcattataa atatagtgat tatatacaat caatgaaaaa
     8161 cgacgtaacg gaatataaag gtaagccaat ataccccgtt gtttttcctg actgggataa
     8221 tgccccaaga tataaagaaa atgcaacttt tttttgtgaa tcttcagcat ttgattttga
     8281 aaaagcattg aatatcgctt gtgatattac gagaaatcat gatgacaagc tgatatttat
     8341 taatgcttgg aatgaatggt ctgaaggagc atatttagaa ccagatgaaa tgtacaaata
     8401 ttctaattta gaaattatta aaaaagtatt tcaggataag agatgaaacg cattgctatt
     8461 ttattagcag catataatgg cgctcgatgg ttagaagagc aaattagctc aatcctacaa
     8521 caaacgaatg ttgaggttac tatatatgtt agtattgatc tgtcaaatga tgaaacttat
     8581 ttattagttt caaagttggc tttaaaggaa cctaggatca taattcttcc ctatggtgat
     8641 atatatggag gggctgcaaa gaatttttac cggttaattt gtgaagtcga ctatgaaaat
     8701 tatgattata tagctttgtc tgatcaggac gacgtatggc gaaaaaacaa actagaacgt
     8761 gcatgtgagt ttttaaaaaa taacgatttt tattctagta atgtcatcgc attttgggaa
     8821 aatgggaaac aagcactaat aactaagtca caagataagg ttgcatttga tcattttttt
     8881 gaatcggcag ggccaggttg tacatttgtt tttaagagag aatatgctct tcaactcaag
     8941 cgtttcttga tgataaataa acatgccatg gatgtagaac ttcatgactg gttgatttac
     9001 gcatttgcac gagaaaataa cttaaaatgg catattgatt cgtatccggg aatgtattat
     9061 cgacaacata aaaataacca agtaggagca aataattcac taaaagcaat tattaaaaga
     9121 attcgactta taaagtccgg atggtatagg aatgaaataa taaagatatt aaatttattt
     9181 gaacaaagtg atattccctt tagagataac ctttgtaata aaaattactt tacctcatta
     9241 tttctcttaa aatatattta tatgataaga cgaaagagaa aagatagagc atttttagtt
     9301 ttaatgatta tttttggtct tttttaactt ataggaatat tcaatgaatt ttgtatcgtt
     9361 agaaaacatg attagcgata ttaggggttt tataccagaa cttgcaattc atgattttga
     9421 cctcattgtc ggtattccaa gaagtgggat gattccagca tatacaattg gattgtattt
     9481 aaatattgat gttacagatg tttcttcttt tgtgaaaaat gtaccattgc aaagaggaat
     9541 ttctagggaa aaaccaacta gccttaagct gcctcaagac gcaaaaaaaa tactattagt
     9601 ggatgatagt tatacaacag gaaaatcgtt agctaatact ctaggcaaaa ttcctatgga
     9661 acttaggaat cgcattgtat cttttgcatt gtataccaca gacagagaaa atcataattt
     9721 agatatgttt atacgggtag taaaataccc acgtgtgttc gaatggaata ttctgaatca
     9781 taacattatt ggcaatagtt gcttagatat agacggtgtg ttatgtatgg atcctacaga
     9841 tgatgataat gatgatggac aaaaatatat aactttcctt gaaaatgcga aaccaaaatt
     9901 tattccaaaa gtgaaaataa agtatcttgt tacaaacagg ttggaaaaat atagaactca
     9961 taccgagaaa tggttatcta aaaataatgt taaatatgag caattaataa tgttagatct
    10021 taaaacaaaa gaagaaagaa taagatctgg acttcattcc acacataagg ctaaatttta
    10081 taaacattct ggttgttcac tttttattga aagtgatgtt aaacaagcat atgaaataat
    10141 gatgcaaagt ggtttgccgg tatattgtat tgatgacaat acaatgtata aaccaggtat
    10201 gacaaaatat tttataaagc aacctagcaa tgcattaaaa aaaatcattc tctggttacc
    10261 aatactaata tataaaaata ttcctaagcc atatcagaaa attattcggc gtttgataaa
    10321 aaaataaaaa gaaccaagtc atatggcttg gttctttgct gataaattac ctttttttaa
    10381 tttttgtttg caatgtttta ataagtttcc tgctttctga tatgggcaat agaagcagta
    10441 aataaatggt tccaccgatt gctgataaaa caaaaagaga tttaatgtca gatgcgaaat
    10501 gaactctaac atttgatatt aatatgaagc ttatagcagc agatataaat agtggataag
    10561 tttttttaaa tatgcttaaa atatttgatt ttattgtttt catcgtgaat ataatacatg
    10621 gaataaaatg tattgcattt gcaatgaaat aatattttgc aagatcaaca attccgcaat
    10681 ggatgcccac cataaatgat accgcagtta atatagttcc ctggatgcct aaaagaagca
    10741 aaatacctgt tttcccttga gacatgaaaa tagtgccagt ggtgcttagc accgactgta
    10801 ttattgcagt gggggcaagc caaactaata tatccgcaga cagaatccat tgttctccaa
    10861 aaacaacttg aatgaaaagt ttgtttaagc tagtaattac tgtaactaat ggtaaagtaa
    10921 taaaccaaat aatatagatt gtatttaaat aaatatcatt tatttctttg taattatttt
    10981 tcttctggct taatatagga tataaagaac gattagcaat aaaagtcaaa gatgagagtg
    11041 ggaatagcat aatccgataa gctaaattat aagatccaag tatagatgct gacatatact
    11101 taccgatcag aaaactatca agatttctgg caaaaaaatt aattatatta aaaagagaaa
    11161 gttgtgaggt aaaacgaaat atttctttta aatgatttat cacatgtcta ggtttgcctt
    11221 taggtctcca tggggatact atccataaca gtactgcaga gctcaaagaa ttaagcagag
    11281 attgaaatac cagactatat attccaaagc tattattagc taatataata gctaataaaa
    11341 gagacaaagc agctgaaaat acttcaattt tagaaataat aaaaaatttc gattctcttt
    11401 caagcatagc tagatgcaat gatgaagagc cggaaataat aaaactaaaa gatagcaaag
    11461 gtaaaacaat tagtagttta ggttgattgt acaaatgtga tataaagggg gtaagtgttg
    11521 taataataat aaataccaaa aagcctaaca aaaaattaag ccaaaaaacg gcacttttag
    11581 tttcatcttg caattgttct ttttgtataa ttgctgctga ggtccctaaa tctcgaaata
    11641 aactagcaaa attcattaca acaccagcca ttgccatgat gccgtattca tttggtggta
    11701 ttattcttgc taaataaaca atgcttatta gctgagttag tattttgaaa acttgtgaaa
    11761 aagtattcca ttttaaatta ttgaaaatgc tcatgtttta atctcactgt tccgattttt
    11821 aaattatacc tgaactgtta gaatgctaca atatttttga ttattgatac taatctctat
    11881 attattgata ccacattggc gattagtttg agtatgttac tacaggcgat ataattttat
    11941 atctaccttg gcttggcgat ttattatttg ttgttaacag aatacctttg taatttatat
    12001 taactgtata tatctatttc ttttatcgtt gaatcaaaat ctgacaggag taaacaatgt
    12061 caaagcaaca gatcggcgtc gtcggcatgg cagtgatggg gcgcaacctt gcgctcaaca
    12121 tcgaaagccg tggttatacc gtctctattt tcaaccgttc ccgtgaaaag acggaagaag
    12181 tgattgccga aaatccaggc aagaaactgg ttccttacta tacggtgaaa gagtttgttg
    12241 aatctctgga aacgcctcgt cgcatcctgt tagtggtgaa agcaggtgca ggcacggatg
    12301 ctgctattga ttccctcaaa ccatatctcg ataaaggcga catcatcatt gatggtggta
    12361 acaccttctt ccaggacacc attcgtcgta atcgtgagct ttctgcagaa ggctttaact
    12421 tcattggtac cggtgtttcc ggcggtgaag aaggtgcgct gaaaggtcct tccattatgc
    12481 ctggtgggca gaaagaagcc tatgaactgg ttgcaccgat cctgaccaaa atcgccgcag
    12541 tggctgaaga cggtgagcca tgcgttacct atattggtgc cgatggtgca ggtcattatg
    12601 tgaagatggt tcacaacggt attgaatacg gtgatatgca actgattgcc gaagcctatt
    12661 ctctgctaaa aggtggcctg aaccttacca acgaagaact ggcacagacc tttaccgagt
    12721 ggaataacgg tgaactgagc agctacctga tcgacatcac caaagacatc ttcactaaaa
    12781 aagatgaaga cggtaactac ttggttgatg tgattctgga tgaagcggct aacaaaggta
    12841 ccggtaaatg gaccagccag agtgcgctgg atctcggcga accgctgtcg ctgattaccg
    12901 agtctgtgtt tgcacgttat atctcgtctc tgaaagatca gcgtgttgcc gcgtctaaag
    12961 ttctctctgg tccgcaagcg cagccagcag gcgataaagc tgagtttatc gagaaagttc
    13021 gccgtgcgct gtatctgggc aaaatcgttt cttacgctca gggcttctct cagctgcgtg
    13081 ctgcgtctga agagtacaac tgggatctga actacggtga aatcgcgaag attatccgtg
    13141 ctggctgcat catccgtgcg cagttcctgc agaaaatcac cgatgcttat gccgaaaatc
    13201 cgcagatcgc taacctgctg ctggctcctt acttcaagca aattgccgat gactaccagc
    13261 aggcgctgcg cgatgtcgtc gcttatgcag tacagaacgg tatcccggtt ccgaccttcg
    13321 ccgctgcggt tgcctattac gatagctacc gtgccgctgt tctgcct
//
