LOCUS       EU296406               15278 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella boydii type 12 O-antigen gene cluster, complete sequence.
ACCESSION   EU296406
VERSION     EU296406.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 15278)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="type: 12"
     gene            558..1643
                     /gene="rmlB"
     CDS             558..1643
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACD37020.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHTDICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALED
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRK"
     misc_feature    558..14294
                     /note="O-antigen gene cluster"
     gene            1643..2542
                     /gene="rmlD"
     CDS             1643..2542
                     /gene="rmlD"
                     /note="dTDP-4-dehydrorhamnose reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACD37021.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVGETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQESDATAPLNVYGETKLAGEKALQEHCVKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEACKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3755..4633
                     /gene="rmlA"
     CDS             3755..4633
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACD37022.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGR
                     DDCALILGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMERGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKVLADPL
                     KKNAYGQYLLKMIKGY"
     gene            4638..5177
                     /gene="rmlC"
     CDS             4638..5177
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACD37023.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTQYYHPECERGIRWDDENIGVDWPLS
                     TDLILSFKDTKHPKFNGIL"
     gene            5179..6270
                     /gene="wfdW"
     CDS             5179..6270
                     /gene="wfdW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37024.1"
                     /translation="MKNYSELLIIHEYGEPSHYQGIIEIAQERRIKVRFLEFSILHNI
                     YAAIKKKKINAVIKAIKDYLILMIYSFFPRMNNGKLVVIGMAPLDKYVFLINKLLKGQ
                     DYIYHTSWLYWDGSDYPKKTYKNPEVLIDEWKTFLNNAKAVACVTPDSAHSLISNFNM
                     KDKTFIVYHSYDSNIFYPSPRQERKKTLTICYLGRLEKNKGIENLIYIIENNDNVNFK
                     IMGSGSYSSKITELSRSKTNVEYLGYLSDKKLIAEQLRQTDIILLPSIKINGWEELFG
                     IALIESMACGVVPITTDHKGPRTILKNKLLAELIFSENEFVESAQKIITDLNNDEFLL
                     QKYKDCAIAEAKFYEKKNISNKWREVIHK"
     gene            6246..7580
                     /gene="wzx"
     CDS             6246..7580
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37025.1"
                     /translation="MEGSYSQIIYDNIFNVYLYELKIMAIINGVFKNIALLISGTAVA
                     QLINFCFIPLITRLYGPESYGMMGSLMAFINIMIPIASLSFPIAIVLPKSDKTAIYIA
                     YLSICIGLVGAGVLLIVIGGLEYLDGSFSQYPEWYILLLPFIMTFLPLQQSGQQWLIR
                     KKQFKEIAKVSVSQALIMNGLKVIGGGILPTQIMIIYTTGAGVIFQCLQFFYRSYKKG
                     LGIPLSKIKKNYFIRVARGYYDFPLYRTPQVLINALSQNLPIFLLGYYFDSKIVGFYV
                     LAQTLLTAPVTLLSTSISNVYYPTIAEKFTQKKPIYNFLRKTIWGLFIVSLFIYLPCI
                     LLSGIVFKIIFGGGWITSSIFFQWMAIYCIFWLSARPAMDTIPPLKIQNYFLLYEVIS
                     FLLRILSLMLGYYLYKDAIICIIMFSSTNAVTYAVLIVYILFQAKVNDRKYT"
     gene            7561..8700
                     /gene="wzy"
     CDS             7561..8700
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD37026.1"
                     /translation="MIENIHNSLQELKSDHINKYNGSNFYYYVSVFGLLLISFRLQIP
                     SLIFIIGAIIIRGTTFKQNISGWLWFGSITFFFGIYLFLGLDNYERGADKFLSRTLVI
                     YLYFVFFYGLGCVRTQNRERLLSFFSLFSVIYVYAVCIYSKISGYPGYNGIYDVFNGN
                     DENSPLYALQLILFTILYLHLNAGKINKIWVGLLIITSFYFSVIYLGSRASFLLLIFY
                     FIFVIFKHKTNLLISFIIFIPFIVTLFIYINDFNFLNYLDFGGFENRGLSSPRFSMLE
                     YGLKNFMNYPWGGLIVHGEGYDGIWLHNMLLDIVRVSGYYTMFVWLFILLMAGLVILK
                     REKFYFPIFLILNIALMQDLAFDGFFNIMALEFFLLGVAVPGLRK"
     gene            8717..9538
                     /gene="wfdX"
     CDS             8717..9538
                     /gene="wfdX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37027.1"
                     /translation="MNMYIVVCVYTLPPAYIKKWFKLHLHNTKYKLIIVDNNLRRQIT
                     DPTVIIGTNTLNEFSAYNEGLQLLKKEFEDEYDIILMLNDTLFTRHNAKFFLKHLLKY
                     KNTVARLSIPAIGGRIDPYNNICYRNPWSNDIGYISSFCIIMNKPARDLYLKLLSDIS
                     PTFPFVDSVTELFNWSTHIDRRFKEFVISHLIDTDTATVWYQSKNNIKNIERLNVKGK
                     CVFLEHYVSGNISKHGVLVSIFPTWKQKTQHFIYEQIAKMERKLLSILNFKVGSK"
     gene            9535..10653
                     /gene="wfdY"
     CDS             9535..10653
                     /gene="wfdY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37028.1"
                     /translation="MKKRILINLLSFSEEKYYGVGVYFRDVAIKSINRVIEELECEIC
                     ILYLAHIPIQKMFTFSKNENIKYIPVKKINSKLTRVFYEQIMLPLYHRDYDVIYSPNN
                     INPLILAFSKKSIITIHDLLPFKRKNRFSLLQRQYLKLFTYLSAKFAYKIITVSNYSK
                     MEIMRLLGTSGDKVIVTYNTLPNIHIENPSINCWINCKYFFSVGALQSDKQYDLMIKA
                     FYKFIQMDEQYKDYKLLIAGGDLGAKKSLCNLIDEFNLTGKVILLGYISELEKNSLYS
                     SCTASLLLGKSEGFGIPVIESMFHNKPVIVSDLGALPEIVGDAGIVVNSNLNDLTNAM
                     VNVCNLNLSEKVFKHELDRFESEKQIECLTSVIKKAIS"
     gene            10650..11471
                     /gene="wfdZ"
     CDS             10650..11471
                     /gene="wfdZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37029.1"
                     /translation="MSENSMFFSLLMAVRKKDDPTFLFEALKSIANNSLQPTEVILVE
                     DGPITRELRKVINGWSNVLNIKSIGLERNSGLGAALNEGLKYCKYEVIVRADADDINR
                     PNRFQCQLNYLLQNPDIDILSSWVEEFSVIPGDKTKVRKVPPQQSLDIFSKKRCPFNH
                     PAVCFRKKTILSLGGYGNEHLYEDYSLWMKMLNSGCKGDNIQEILVDMRFSDEALKRR
                     GGIKYALSELRTQWNFYVLNYISLPRFVSNAICRTSVRIIPNKIRVSLYKIFLRS"
     gene            11474..12910
                     /gene="manC"
     CDS             11474..12910
                     /gene="manC"
                     /note="mannose-1-phosphate guanylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ACD37030.1"
                     /translation="MKEKQIIPVILCGGNGTRLWPLSREQYPKQFLSLQGNVSMLQAT
                     ISRLANLKCSEPIVVCNDKHRFIVAEQLKKIGKLSNNIVLEPEGKNTAPAIALSAFIA
                     EKITNISSAILLVLAADHIIENESVFIETIKKTISLAQLGKLVTFGITPRYAETGYGY
                     IHRGNKVNNNDVIAYQVSNFVEKPNLTLARKYLKNGEYYLNSGMFLFRVDIYLKELKK
                     YRPEIYRICLSSVKNIGHDLDFIRINASQFNKSPSESIDCAVMENTSDAVVVPMDVGW
                     SDVGSWESLWDVSSKDEHGNFSKGNVISFDAHDNYIYAESSLVTTLGIENLIVIQTKD
                     ALLVADKKKSQEVKLIVDKLKNENLKEYSLHREVHRPWGKYNYLCEGVGYQVKHILVN
                     PGESLSLQMHYYRVEHWIVISGTAKVQINSEEKIISENESIFIPSGVKHSLENFGEVP
                     LIIIEVRTGEYFGEDDVIRFDNYHSLDK"
     gene            12924..14294
                     /gene="manB"
     CDS             12924..14294
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ACD37031.1"
                     /translation="MNMLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     GYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRGAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELNKVHNTPDGNFPNGIP
                     NPLQPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAISR
                     LEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLSK"
BASE COUNT     4646 a   2630 c   3312 g   4690 t
ORIGIN
        1 atccaactaa ctgatgctat tgccgaactg gcgaaaaaac agtccgttga tgccatgctg
       61 atgaccggcg acagttacga ctgcggtaaa aaaatgggct atatgcaggc gttcgtgaag
      121 tatggattac gcaacctgaa agaaggggcg aagttccgca aaggcattga gaagttgtta
      181 agcgaataat gaaaatctga ccggatgtaa cggttgataa gaaaattata acggcagtga
      241 agattcgtag caaaagtaat ttgttgcgaa tattcctgcc gttgttttat ataaatattc
      301 agaataacaa cgagttagca ataggatttt agtcaaagtt ttccaggatt ttccttgttt
      361 ccagagcgga ttggtaagac aattagcgtt tgaatttttc gagctaagcg cgagtgggga
      421 acgctcgcta catcgtaggc atgcagtgtt ctggtagctg taaagccagg ggcggtagcg
      481 tgcattaata cctctattaa tcaaactgag agccgctaat ttcacagcat gctctgaagt
      541 aatatggaat aaattaagtg aaaatacttg ttactggtgg cgcaggattt attggttctg
      601 ctgtagttcg tcacattata aataatacgc aggatagtgt tgttaatgtc gataaattaa
      661 cgtacgccgg aaacctggaa tcacttgctg atgtttctga ttctgaacgc tatgtttttg
      721 aacatacaga tatttgcgat gcagctgcaa tggcacggat ttttgctcag catcagccgg
      781 atgcagtgat gcacctggct gctgaaagcc atgttgaccg ttcaattaca ggtcctgcgg
      841 catttattga aaccaatatt gttggaactt atgtcctttt agaagccgct cgcaattact
      901 ggtctgctct tgatagcgac aagaaaaata gcttccgttt tcatcatatt tctactgacg
      961 aagtctatgg tgatttgcct catcctgacg aggtaaataa tacagaagaa ctacccttat
     1021 ttactgagac gacagcttac gcgccaagca gcccttattc cgcatccaaa gcatccagcg
     1081 atcatttagt ccgcgcgtgg aaacgtacct atggtttacc gaccattgtg actaattgct
     1141 ctaacaatta tggtccttat catttcccgg aaaagcttat tccattggtt attcttaatg
     1201 ctctggaaga taaagcatta cctatttatg gtaaagggga tcaaattcgc gattggctgt
     1261 atgttgaaga tcatgcgcgt gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa
     1321 cttataacat tggtgggcac aacgaaaaga aaaacataga tgtagtgctc actatttgtg
     1381 atttgctgga tgagattgta ccgaaagaga aatcttatcg tgagcaaatc acttatgttg
     1441 ccgatcgtcc gggacacgat cgccgttatg ccattgatgc tgataagatt ggtcgtgaat
     1501 tgggatggaa accacaggaa acgtttgaga gcggcattcg taaaacggtg gaatggtacc
     1561 tgtccaatac aaaatgggtt gataatgtga aaagtggtgc ctatcaatcg tggattgaac
     1621 agaactatga gggccgcaag taatgaatat cctccttttc ggcaaaacag ggcaggtagg
     1681 ttgggaacta cagcgtgccc tggctcctct gggtaatttg attgctcttg atgttcactc
     1741 cactgattat tgtggtgatt ttagtaatcc tgaaggtgta ggtgaaaccg taagaagcat
     1801 tcggcctgat attattgtca acgcagccgc tcacaccgca gttgacaaag cagaatcaga
     1861 accggagttt gcacaattac ttaacgcgac aagtgtcgaa gcgattgcga aagcagccaa
     1921 tgaagtcggt gcctgggtta ttcactactc tactgactat gtatttccgg gaaccggtga
     1981 aataccatgg caggagtcgg atgcaaccgc accgctaaat gtttacggtg aaaccaagtt
     2041 agccggagaa aaagcattac aagagcattg tgtgaagcac cttattttcc ggaccagctg
     2101 ggtctatgca ggtaaaggaa ataacttcgc caaaacgatg ttacgactgg caaaagagcg
     2161 tgaagaattg gccgttatta acgatcagtt tggtgcgcca acaggtgctg agctgctggc
     2221 tgattgtaca gcacatgcca ttcgtgtcgc actgaataaa ccggaagtcg caggcttgta
     2281 ccatttggta gccagtggta ccacaacctg gcacgattat gctgcgctgg tttttgaaga
     2341 ggcgtgcaaa gcaggcattc ccctcgcact caacaagctc aacgctgtac caacaacagc
     2401 ctatcctaca ccagcacgtc gtccacataa ctctcgtctt aatacagaaa aatttcagca
     2461 gaattttgcg cttgtattgc cagactggca ggttggcgtg aaacgcatgc tcaacgaatt
     2521 atttacgact accgcaattt aatagttttt gcatcttgtt cgtgatggtg gagcaagatg
     2581 aattaaatag ctgcgcttaa taccgctaca cttttgccag tccatgtttg cctccgggga
     2641 atgggctgac ggtttccata aaatggcgaa cttttttcaa cagttgccac attgagcggc
     2701 actgatgatt acgcgttatt gtgtcgtgaa gtgcctgcca tagccgttca acatgattca
     2761 cccatggcga gtaaaccggc tgataaatga ccctgaactt cgggttctcc ttcagccagc
     2821 tctgtgtttc ccggtttttg tggataatgt agttgtccac gatcagcgtg atggttttcg
     2881 cccgacggta tgtcgcttta agccgcttca gcaggctgat gaacagcgcc gaacttttgc
     2941 tgttgccgcc cacatagctg actttacctg tcccgctgtg cagcgctccg gccagataat
     3001 atttttcatt ctgtcccggc gtgaccaccc gtttttgctg tccgcgcagt tgccagtctg
     3061 caccgatttt gggattaaga tggatatcca cttcatcttc ataaaagacc ggatgttctg
     3121 cgctgcattc gtccagtgct ttatggattg ctgccatctt ttcatcttta tgcgggtcac
     3181 ggatacgcag agttggcgca gcccttcgcc acacaatccc cgcagacggc aaccagcggc
     3241 gaacggttcc ggcatttaac tggcaaccgg ttatctcatt gatttttatt gccagcagtt
     3301 ctgtactcca gcgtgaacgc tggtagccaa agtcgccggg agaatgtttt accagctcac
     3361 gtaacagtgt gcagatatgc tcaaacggcc agcgacgggc acgcccggca ggtaatgatt
     3421 tcagtccctc aacacccgac tgcgtgaacc agttaatcca gcgtccaaca gaggagcggg
     3481 cgcagcagag cgttctggca acgtcgctga cacggtcgcc ccggtgcagc atcagcatgg
     3541 cagtcagtct gcgggcataa tttctatcgt gtgttttatg gatggctttc tgcatcaggc
     3601 gtcgttcgtc acgggaaatt ggtgctatga tcggcattgc tcagtccggt tggtgatttg
     3661 ttttgatttg gcgattgatc agatcgcaca atccgggcta agttccctca aagtgatcta
     3721 ctattccgcg cagctattta aaaggaatga tgaaatgaaa acgcgtaaag gtattatttt
     3781 agcgggggga tctggtactc gtctttatcc tgtcactatg gctgtcagta aacagctgtt
     3841 accgatttat gataaaccga tgatttatta tccgctatct acactgatgt tagcgggtat
     3901 tcgcgatatt ctgattatca gtacgccaca ggatactcct cgttttcaac aactgctggg
     3961 tgatggtagc cagtggggcc ttaatcttca gtataaagta caacctagtc cagatggttt
     4021 ggcacaagct ttcattattg gagaagattt tattggtagg gatgattgcg cattaattct
     4081 aggtgataat atcttttacg gtcatgacct accaaaatta atggatacag ctgttaatag
     4141 agagagtggt gcaacggtat ttgcctatca cgttaatgat ccagaacgtt acggtgtggt
     4201 ggaatttgat aaaaacggca cggcaataag cctggaagaa aaaccgcttc aaccgaaaag
     4261 caactatgca gttacagggc tttatttcta tgataatgac gtcgtggaaa tggcgaaaaa
     4321 tcttaagcct tctgcccgtg gtgaactgga aattaccgat attaatcgta tctatatgga
     4381 acgggggcgt ttgtctgttg ccatgatggg ccgtggttat gcatggctgg acacggggac
     4441 acatcaaagc ctgattgagg caagcaactt catcgcaaca attgaagagc gtcaggggtt
     4501 aaaagtttcc tgcccggaag aaattgctta ccgtaaaggg tttattgatt ctgagcaggt
     4561 gaaagtatta gctgatccgc tgaaaaaaaa tgcttatggt cagtatctgc ttaaaatgat
     4621 taaaggttat taataaaatg aacgtaatta aaacagaaat tccagatgta ctgatttttg
     4681 aaccgaaagt ttttggtgat gagcgtggtt tcttttttga gagctttaac cagaaggttt
     4741 ttgaggaagc tgtaggccgc aaagttgaat ttgtccagga taaccattcg aagtcttgta
     4801 aaggtgtttt acgcggactg cattatcaat tagaacctta tgcgcaaggg aaattggtgc
     4861 gctgcgttgt tggtgaagtt tttgacgtag cggttgatat tcgtaaatcg tcacctactt
     4921 ttggcaagtg ggttggggtg aatttatctg ctgagaataa gcgccagttg tggatcccgg
     4981 aggggtttgc tcatggtttt ttggtgctga gtgagacggc ggagtttgtt tataagacga
     5041 ctcagtatta tcatcctgaa tgtgaacgag ggatcagatg ggatgatgaa aatatagggg
     5101 tagattggcc tctgtctaca gatttaatac tatcttttaa agataccaag catccaaaat
     5161 tcaacggtat tttataaaat gaaaaattat agtgaattat taataatcca tgaatatgga
     5221 gaaccatctc attaccaagg aataatagag attgctcagg agagaagaat taaggtccgt
     5281 tttttagaat tcagtatttt acataatatt tatgctgcaa ttaaaaaaaa gaaaataaat
     5341 gccgttataa aggcgatcaa agattatttg atattgatga tatactcatt ctttccaaga
     5401 atgaataatg gaaagttggt tgttataggc atggccccgt tagataaata tgttttttta
     5461 ataaataaat tattgaaggg acaagattat atttatcaca catcatggct ttattgggat
     5521 ggcagtgact atcctaaaaa aacatataaa aatcctgagg tattaattga tgagtggaag
     5581 acttttttaa ataatgctaa agcggttgca tgtgtaacac ctgattctgc gcatagttta
     5641 atttcaaact ttaatatgaa agataaaaca ttcattgttt atcattcgta tgactcaaat
     5701 attttctatc cttcgccaag gcaagaacga aaaaaaacat taacgatatg ttacttaggg
     5761 cgacttgaaa aaaacaaagg aattgaaaat cttatttata ttattgaaaa taatgataat
     5821 gttaatttta aaataatggg cagcggaagt tactcaagca aaataactga gttaagcagg
     5881 tcaaagacta atgttgaata cttagggtac ctatcggata aaaaattaat agcagaacaa
     5941 ttgcggcaaa ctgatattat attacttcca tcaattaaaa ttaacgggtg ggaagaatta
     6001 tttggaatcg cattaataga gtcaatggct tgtggggtag tacctattac tactgatcac
     6061 aaaggtccta ggaccatact aaagaataaa ttattagcag agctcatttt cagtgagaat
     6121 gaatttgttg agtcggcaca gaagatcatc acagatttaa ataatgatga atttttatta
     6181 caaaagtaca aggattgtgc gattgctgag gcaaagtttt atgaaaagaa aaatatatca
     6241 aataaatgga gggaagttat tcacaaataa tttatgacaa catttttaat gtctatcttt
     6301 atgagttgaa aataatggcc ataattaatg gtgtttttaa aaatattgct ttgcttattt
     6361 ctggaaccgc agtagctcaa ttaattaatt tttgttttat tcctttgatt acaagattgt
     6421 atggtcctga gtcttatgga atgatgggaa gcttaatggc gtttattaac attatgattc
     6481 cgatagcttc attaagtttt ccaattgcaa ttgtgttacc aaagtctgat aaaaccgcta
     6541 tttatattgc ttaccttagc atttgtatag gtttggtggg tgcaggagtt ctattaatcg
     6601 taataggggg attggaatat ttagatggaa gtttcagtca ataccctgaa tggtatatat
     6661 tactattacc atttatcatg acattcctac ctctacagca aagtggacag caatggctaa
     6721 taaggaaaaa acagtttaaa gaaatagcaa aagtatctgt cagtcaagca ttaattatga
     6781 atggtttgaa ggtaatcggg ggagggattt taccaacaca aatcatgata atatatacaa
     6841 caggtgcagg agtaattttt caatgcttac agttctttta tcgatcttat aagaaagggc
     6901 ttggaattcc tttatcaaaa ataaaaaaga attattttat aagggttgcg cgcggctatt
     6961 atgattttcc gctatataga actccgcaag tattaatcaa tgcgttgtct caaaatttgc
     7021 cgatattttt gttggggtat tattttgatt ctaaaatagt aggcttttat gtgcttgctc
     7081 aaacgttact aactgctcca gtgactttgt taagcacttc gataagtaat gtatattatc
     7141 ctacaatcgc agagaaattt acccaaaaaa aacctattta taatttctta agaaaaacaa
     7201 tttggggact gtttattgta tccttgttta tctatttgcc atgtatattg ttatctggca
     7261 ttgtatttaa aattattttt ggaggtgggt ggattacgtc tagcattttt tttcaatgga
     7321 tggctattta ttgcattttt tggttaagtg ctagacccgc gatggatact ataccgccat
     7381 tgaaaattca aaactacttt cttctatatg aagttatttc ttttttattg aggattttaa
     7441 gcctaatgtt aggctattat ttatataaag atgcgataat ttgtattatt atgttttcat
     7501 caaccaatgc agtcacatat gctgtcttga tagtttatat tttatttcag gcaaaagtaa
     7561 atgatagaaa atatacataa ttcactacaa gagttaaaga gtgatcatat taataaatat
     7621 aatggctcaa acttttatta ctatgtctca gtatttggtc tattacttat ctcatttcga
     7681 cttcagatac cctcacttat atttattata ggtgcgatta ttataagagg tactactttt
     7741 aagcaaaata tatctggttg gctttggttc ggaagtatta cttttttttt tggaatatat
     7801 ctttttttgg ggttggataa ttatgaaaga ggtgctgata agtttttatc acgaacttta
     7861 gtaatatatc tttatttcgt ttttttttac gggttgggat gtgttagaac tcaaaataga
     7921 gaacgactct tatctttttt ttcattattt agcgtgattt atgtctatgc tgtctgtatc
     7981 tatagtaaga ttagcggtta tccaggatat aatggaatat atgatgtgtt caatgggaat
     8041 gatgaaaatt ctccattata tgcattacag ttgattctat ttacaatttt atatcttcac
     8101 ctcaatgcag ggaaaataaa taaaatatgg gttgggttgt taattatcac atctttttat
     8161 tttagtgtta tttatcttgg ctctcgagca tccttcttat tattaatttt ttatttcatt
     8221 tttgtgatct ttaaacataa aactaacttg ttaatatctt tcattatttt tatcccgttc
     8281 atagttacat tatttattta tataaatgac tttaattttt taaattattt agattttgga
     8341 gggtttgaaa atcgaggact aagcagtcct cgatttagta tgcttgaata tggtttaaag
     8401 aatttcatga attatccatg gggaggttta attgttcatg gtgaaggtta tgatggtatc
     8461 tggctccata atatgttatt agatatagtt agggtatcag gttactatac tatgtttgta
     8521 tggctgttca ttttattaat ggcaggttta gttattctga aacgtgaaaa attttatttt
     8581 ccaatatttc taattctaaa tatagcttta atgcaagatt tagcatttga tggttttttt
     8641 aacataatgg ctctggagtt ttttttattg ggtgtagctg tacctggttt aagaaaataa
     8701 aggaaaataa aatataatga atatgtatat tgtggtttgt gtatataccc tgcccccagc
     8761 gtatataaaa aaatggttta agttacattt gcataatact aaatataaac ttattattgt
     8821 cgataacaac ttaagaagac aaataacaga tccaactgtt attattggca caaatactct
     8881 taatgaattc tcagcataca atgaagggct tcagctacta aaaaaagaat ttgaagatga
     8941 atatgacata atattgatgt tgaatgatac tttgttcact aggcataatg cgaaattttt
     9001 cttaaaacat ttattaaagt ataaaaatac agttgctcgc ttatcaatcc cggctatcgg
     9061 tggaagaata gatccatata ataatatctg ttatagaaac ccatggagca atgacattgg
     9121 ttatatatct tcattttgta taataatgaa taaaccagca agagatctat atttgaagtt
     9181 actatctgat atatccccaa cttttccatt tgttgattca gttactgagc ttttcaactg
     9241 gagtactcat atagacagaa gatttaaaga atttgtgatc agtcatctga tagatacaga
     9301 cacagctact gtgtggtacc aatccaagaa taatattaaa aatatagaaa gattgaatgt
     9361 taaaggtaag tgtgtttttc ttgaacatta tgtatcagga aatatttcta aacatggagt
     9421 attggtttca atctttccca cgtggaagca gaaaactcag cattttattt atgagcaaat
     9481 cgcaaaaatg gaaagaaaat tattatcgat actaaatttt aaagttggtt caaaatgaaa
     9541 aaaagaattt taataaatct attgagtttt tctgaagaaa aatattacgg tgtgggagtt
     9601 tatttcagag atgttgctat taaaagtatt aacagggtta ttgaagaact agaatgcgaa
     9661 atatgtattt tatatttggc acatattccg atacaaaaga tgtttacttt ttcaaaaaat
     9721 gaaaacataa aatatatacc tgtaaagaaa attaatagca aactcacaag agttttctac
     9781 gaacaaataa tgttaccttt gtatcataga gattatgatg tgatttatag tcctaataat
     9841 ataaatccat taatattagc tttttcaaaa aaatctataa taactatcca cgatttgcta
     9901 ccattcaaga gaaagaatag atttagttta ttgcagagac aatatttgaa actatttact
     9961 tatctatctg caaagtttgc atataaaata ataactgttt ctaattattc caagatggag
    10021 attatgcgtc ttcttggtac cagtggtgat aaagttatag tgacttataa tacgttacca
    10081 aatatacata tagagaatcc atcaattaat tgttggatta attgtaaata ctttttttct
    10141 gttggagcat tgcaatctga taaacagtat gatttgatga ttaaagcttt ctataaattt
    10201 attcaaatgg atgagcagta taaagattac aaattgctta ttgctggtgg ggatttagga
    10261 gcaaaaaaat ccttatgtaa cctaattgat gagtttaatt taaccggcaa ggttattctc
    10321 ttagggtata tttcagaact cgaaaaaaat tcactatata gttcatgtac tgctagcctg
    10381 ttactaggaa aaagtgaggg attcgggatt cctgtgatcg aaagtatgtt ccacaacaaa
    10441 cctgtaattg tttcagatct gggggcatta cctgaaatag ttggtgatgc tggaatcgtt
    10501 gttaactcga atctaaatga tttaacaaat gctatggtaa atgtatgtaa tttgaacctg
    10561 tcagagaaag tatttaagca tgagttagat aggtttgaaa gtgaaaagca gattgaatgt
    10621 ctgacttctg taattaaaaa ggctatttca tgagtgaaaa ttcaatgttc ttttctcttt
    10681 tgatggctgt ccgtaagaaa gatgatccaa cctttttgtt tgaggcactt aaatcaattg
    10741 caaataatag tctacaacct acagaggtaa tcttggtcga agatggacct attactcggg
    10801 aattaagaaa agttattaac ggttggtcaa atgttttaaa tattaaaagt attggtttag
    10861 agagaaactc tggattaggt gctgctctta atgaagggct taaatattgt aaatatgaag
    10921 tgattgtgcg agccgatgct gatgatatta accgaccaaa tagatttcag tgccaactaa
    10981 attatttatt gcaaaatcct gatattgata tattaagttc gtgggtcgag gaattctcag
    11041 tgatacctgg tgataaaact aaagtgagaa aagttcctcc tcaacaatca ttagacattt
    11101 tttcaaagaa aagatgtcca tttaatcatc cagctgtatg ttttagaaaa aaaactatac
    11161 tgtcactcgg tggctatggt aatgagcatt tatatgagga ttactcatta tggatgaaaa
    11221 tgcttaattc cggatgcaaa ggggacaata tacaagaaat attagttgat atgcgttttt
    11281 ctgatgaggc attgaaacgc aggggaggga taaaatatgc tttatcagaa ttaagaactc
    11341 aatggaattt ttatgttttg aattatattt ctcttccaag gtttgtttca aatgctatat
    11401 gtcgtaccag tgtcagaatc attccaaata aaattcgtgt gagtttatac aaaatctttc
    11461 ttcggagcta actatgaaag agaaacaaat tatcccagta attttatgtg gtggaaatgg
    11521 tactagactc tggccattgt ctagggaaca ataccctaag caatttcttt ctttgcaagg
    11581 gaatgtgtca atgttacagg ccactatttc aagattagct aatctaaagt gctccgaacc
    11641 gatagtcgtt tgtaatgata agcatcgctt tatcgttgca gaacaactaa aaaaaatcgg
    11701 taaattatcg aataatattg tactggagcc tgaaggtaag aatactgcgc cagcaattgc
    11761 attgtctgcg tttatcgctg agaaaattac aaatatatca tcagctatat tgttagttct
    11821 ggctgcagat catattatag agaatgaaag cgtttttatt gagactataa aaaaaaccat
    11881 ttcgttagct caattaggaa agctggttac tttcggaatt acccctagat atgcagaaac
    11941 gggttacggc tatattcata gaggtaataa agtaaacaat aatgacgtta tcgcttatca
    12001 agtgtctaat tttgttgaaa aaccaaatct aactttggca cgaaaatatt tgaaaaatgg
    12061 agagtattat ttgaatagcg gtatgttttt atttcgcgta gatatttatt taaaggaatt
    12121 gaagaaatat agaccagaaa tatatcggat ttgtttgagt tctgtaaaaa atatagggca
    12181 tgatttggat tttattagga ttaatgcatc tcaatttaat aaaagcccat cggagtctat
    12241 cgattgtgca gtcatggaaa atacaagtga tgcagttgtc gttcctatgg acgttggatg
    12301 gagtgatgtc ggatcatggg aatcattatg ggatgttagc agcaaggatg aacatgggaa
    12361 ttttagcaaa ggaaacgtta ttagttttga tgctcatgat aattatatat atgctgaatc
    12421 atcgctggta acgacactcg gaatcgaaaa cttaattgtt attcaaacga aagacgcttt
    12481 gcttgttgct gataaaaaaa aatctcaaga agtaaagcta attgttgata aacttaaaaa
    12541 tgaaaatctg aaagagtatt ctttacatcg agaagttcat agaccatggg gtaaatataa
    12601 ttatctttgt gaaggcgtag gataccaagt taagcatata ttagttaatc cgggagagtc
    12661 actgtcactt caaatgcatt attatagagt agagcattgg attgtcattt ctggaacagc
    12721 taaagtccaa ataaactcag aagaaaaaat tattagtgaa aatgaatcaa tatttattcc
    12781 atcgggagtt aaacatagtt tggaaaattt tggggaagta ccattaataa taatcgaagt
    12841 aagaactggc gagtattttg gagaagatga tgttattaga tttgataatt atcatagttt
    12901 agataaatag tggttactta agaatgaata tgcttacttg ttttaaagcc tatgatattc
    12961 gcggaaaatt aggcgaagaa ctgaatgaag atatcgcctg gcgtattggg cgtgcctatg
    13021 gcgaatttct caaaccgaaa accattgttt taggcggtga tgtccgtctc accagcgaaa
    13081 ccttaaaact ggcgctggcg aaagggttac aggatgcggg cgtcgacgtg ctggatatcg
    13141 gcatgtccgg caccgaagag atctatttcg ccacgttcca tctcggtgtg gatggcggca
    13201 tcgaagttac cgccagccat aacccgatgg gttataacgg catgaagctg gtgcgcgagg
    13261 gggctcgccc gatcagcggt gataccggac tgcgcgacgt ccagcgtctg gcagaagcca
    13321 acgactttcc tcccgttgat gaaaccaaac gcggtcgcta tcaacaaatc aatctgcgtg
    13381 gcgcttacgt tgatcacctg ttcggttata tcaatgtcaa aaacctcacg ccgctcaagc
    13441 tggtgatcaa ctccgggaac ggcgcagcgg gtccggtggt ggacgctatc gaagcccgct
    13501 ttaaagccct cggcgcacct gtggaattga acaaagtgca caacacgccg gacggcaatt
    13561 tccccaacgg tattcctaac ccgctgcagc cggaatgtcg cgacgacacc cgcaatgcgg
    13621 tcatcaaaca cggcgcggat atgggcattg cctttgatgg cgattttgac cgctgtttcc
    13681 tgtttgacga aaaagggcag tttatcgagg gctattacat tgtcggcctg ctggcagaag
    13741 cgttcctcga aaaaaatccc ggcgcgaaga tcatccacga tccgcgtctc tcctggaata
    13801 ccgttgatgt ggtgaccgcc gcgggcggca ctccggtgat gtcgaaaacc ggacacgcct
    13861 ttatcaaaga acgtatgcgc aaggaggacg ccatctacgg tggcgaaatg agcgcccacc
    13921 attacttccg tgatttcgct tactgcgaca gcggcatgat cccgtggctg ctggtcgccg
    13981 aactggtgtg cctgaaagag aaaacgctgg gcgaactggt acgcgaccgg atggcggcgt
    14041 ttccggcaag cggtgagatc aacagcaaac tggcgcaacc cgttgaggcg attagccgct
    14101 tggaacagca ttttagccgt gaggcgctgg cggtggatcg caccgatggc atcagcatga
    14161 cctttgccga ctggcgcttt aacctgcgca cctccaatac cgaaccggtg gtgcggttga
    14221 atgtggaatc gcgcggtgat gtgccgctga tggaagaaaa aacaaaactt atccttgagt
    14281 tactgagtaa gtaatccagt aatttcatat aaatgggttt taaaagacgg aaaagatgag
    14341 atatccagtg tggtatagcc aaggtaatgc tattcagcat ctctatgagt gagttaacat
    14401 ctataccaca tttaagccgc acactcggcg gtgaccaccc cctgacagga gtaaacaatg
    14461 tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct tgcgctcaat
    14521 atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt cccgtgagaa aacggaagaa
    14581 gtgattgccg aaaatccagg caagaaactg gttccttact acacggtgaa agagtttgtt
    14641 gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat
    14701 gctgctattg attctctcaa gccatacctc gataaaggtg acatcatcat tgatggtggt
    14761 aacaccttct tccaggacac cattcgtcgt aaccgtgagc tgtctgccga aggttttaac
    14821 ttcattggta ccggtgtttc cggtggtgag gagggcgcac taaaaggtcc ttccattatg
    14881 cctggtggcc agaaagaagc ctatgaactg gttgctccga tcctaaccaa aatcgccgca
    14941 gtggctgaag acggtgagcc atgcgttacc tatattggtg ccgatggtgc aggtcactat
    15001 gtgaagatgg ttcacaacgg tattgaatac ggcgatatgc agctgattgc tgaagcctat
    15061 tctctgctta aaggtggcct gaacctcacc aacgaagaac tggcgcagac ctttaccgag
    15121 tggaataacg gtgaactgag cagctacctg atcgacatca ccaaagatat cttcaccaaa
    15181 aaagatgaag agggttacta tctggttgat gtgattctgg atgaagcggc taacaaaggt
    15241 accggttaat ggaccagcca gagtgcgctg gatctcgg
//
