LOCUS       AY369140               14504 bp    DNA     linear   BCT 02-JAN-2004
DEFINITION  Shigella boydii type 13 O antigen gene cluster, partial sequence.
ACCESSION   AY369140
VERSION     AY369140.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Feng,L., Senchenkova,S.N., Yang,J., Shashkov,A.S., Tao,J., Guo,H.,
            Zhao,G., Knirel,Y.A., Reeves,P. and Wang,L.
  TITLE     Structural and genetic characterization of the Shigella boydii type
            13 O antigen
  JOURNAL   J. Bacteriol. 186 (2), 383-392 (2004)
   PUBMED   14702307
REFERENCE   2  (bases 1 to 14504)
  AUTHORS   Feng,L., Senchenkova,S.N., Yang,J., Shashkov,A.S., Tao,J., Guo,H.,
            Zhao,G., Knirel,Y.A., Reeves,P. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2003) Microbiology, College of Life Sciences,
            Weijin Street, Tianjin 300071, P.R.C.
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /strain="M1350"
                     /serotype="13"
                     /db_xref="taxon:621"
     gene            <1..765
                     /gene="galF"
     CDS             <1..765
                     /gene="galF"
                     /note="GalF"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="AAR24268.1"
                     /translation="IVAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLA
                     EVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRY
                     NLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQ
                     TLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSY
                     DCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLLE"
     misc_feature    942..1319
                     /note="non-functional transposase protein due to mutation"
     mobile_element  942..1319
                     /note="remnant"
                     /mobile_element_type=insertion sequence:IS1004
     gene            1713..3032
                     /gene="wzx"
     CDS             1713..3032
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAR24269.1"
                     /translation="MKSAIRSGKEQFLMGVIYKALFMLITSINALVIVKLLTPEELGI
                     WYIFMALQTLLFILNSALTPNISRQYSIAFTDIGEGFDPRLFHTKVIIIYNKIILVAT
                     VMGVFFTLTYLNYVLDIFRLANVSILLSWVIVFASILMEIYFTSYECALNGFGEFQKV
                     NKVNFISRLILTLSIFTLLLSDVHSNGLLIFCGLYFFSNIIKRVMVINLFNHTFKDKG
                     LKESRKTIDFFDRISCKLQKLSFLSLLSSIGGVLIVRSGMFILPFYVPMDQVASYGLT
                     YQLFELGFNVIFTISIIATPAWISAYKNNPEGLKKSFTWVKYISLLLMLICGLLITFL
                     GNIILEFADVETRVQSVILCFYLLVIFVLQINHSISGQLLTIQNKIPFAYASFLSGIF
                     TLVLSVVLIKYYSVLGAIYAILISQLIYNNWKWPYEAYKFLRVHGNV"
     gene            3019..4197
                     /gene="wzy"
     CDS             3019..4197
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAR24270.1"
                     /translation="MEMYEGKLKNKILSLFLLCETFCFIYIIYNKELVSDYLGRAITL
                     SEFTISCIFFLLLLFYFLFHTLWVISEKIIKSRKKIGSVGNLNLFVLVLIFLYFILNL
                     KTGIGKVGQEDNITLTGLDKLLFAAPIFFKINFLMYIYAAGNRNKNRLYYFNLFFFAF
                     AELTRGVSFTILLLLIIEYNKIRRFISLRYFLFSLLFGILLINVIYNVKFYVRMGAKY
                     EYIDIYTSLIMLMGRLSILSNLLFIHDNLSDIRLYFYGSGYQGVVHEFLESLTPVPSL
                     FGINEKVLEFGKILFSYSNVTFTSATAASLLGIIYIYPNSIFNAITIICLSWIYIHII
                     TGYLEFSRMQRVVAYFFVLLALYQGFMAILSNYIYALTIYFLIACARNLLLSKKYETI
                     "
     gene            4184..4969
                     /gene="wbwE"
     CDS             4184..4969
                     /gene="wbwE"
                     /note="WbwE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAR24271.1"
                     /translation="MKQFSIKILSLADAIERRRKVSREFELKSVLPFSFFNGIYGKTL
                     SKEKLDTIYSSELAQKVLHRQLTAGEIGATYSHYLIFREAYERGDEFVVVLEDDCYID
                     RNFDTVLTSILDKKNPTDDEIIFIQRHTSENSHIIRSLGSEKINKNYTLHRMLGSAQY
                     FVGAYGYIVTRAAMKKMIDTYLPIYCVCDHWYFIKKKCQIEKFSVLSPAIVFTNDEDV
                     RKIDSFVDIERREIAVFKSVGKIAKLKILIKKIILPILNKDWE"
     gene            4972..6042
                     /gene="wbwF"
     CDS             4972..6042
                     /gene="wbwF"
                     /note="WbwF; D-Glc-1-P transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAR24272.1"
                     /translation="MLRYYKVLTVLLRLIIAFILYPIAYVFLNHKKYFIIGTRNGVRG
                     HDNAEYFLSYCTRHNINTKVIYNDAYQKDELCKNSIKSILYILCANNVYITHSESDVL
                     DYFWRFIPGIKYIFIQHGVIGIKKLPDYEKKRYHRYVSSSEYETEIFRNFFHLNSDKI
                     IKSGLPRFDFYDYNRSKNDEIKSCLIMFTWRKSDSGKAKLIKKYTEMVTCLSNEALNK
                     IYICVHEANLSTFKVLLEESLELDDRFSFIENDKLSCIIQSTELLITDYSSVAWDYLY
                     QNKYILFYTPDIDDYKNTTGLYCDFSDFFGAHLMNFSRINYPVLAWIMQENEIKNKNF
                     LARYKFYNMHAGIHSEYIVKNS"
     gene            6138..7172
                     /gene="wbwG"
     CDS             6138..7172
                     /gene="wbwG"
                     /note="WbwG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAR24273.1"
                     /translation="MSNLKKYKFIIFIPDSINLPKAVNIRYIPVPKKNWLSRIYWDSY
                     GLRKYIRVNRLQYQAVISLQNTSVNVEGKQIIYLHQSIPFIDFDIPLTSLFNLKLWLY
                     KRFYSYFIFLFVNKNTSFIVQAKWLKELLSDKYHIDAKKISIIKPKARYVPLATNSIH
                     NVANLSKQFCNIIYPATPIFYKNHCVIIDALRILREQRNIDNLCFNVTFSKGEYAKFD
                     DLVRRYNLGKNIRYLGYLTRDELYQHYDNSAFMVYPSYVETCGLPLLEAASKQLPIIA
                     SDLPYACEMLEAYTGVVYVKFNVSTEWAREIDTMARAGETRIIPPLLKAENDSDWNKL
                     DNIIEGKDDV"
     gene            7162..8196
                     /gene="fnlA"
     CDS             7162..8196
                     /gene="fnlA"
                     /note="FnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="L-QuiNAc synthase"
                     /protein_id="AAR24274.1"
                     /translation="MMFKNKTLLITGGTGSFGNAVLRRFLSTDIGEIRIFSRDEKKQD
                     DMRKKYSDPKLKFYIGDVRDYNSILSATRGVDYIYHAAALKQVPSCEFYPMEAVKTNV
                     IGTDNVLEAAIANKVQRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPEGITIC
                     ATRYGNVMASRGSVIPLFINQIKNGNPITITDPDMTRFMMTLDDAVDLVLHAFEYGNN
                     GDIFVQKAPAATIETLTKAIQQVTNALDHQINIIGTRHGEKLYEVLCSREEMAVAEDQ
                     GNYYRIPADNRDLNYEKYFEKGNKDVSLLEDYNSHNTIRLDVDGMVALLRKLEFIRKV
                     EAGYDANPDE"
     gene            8200..9075
                     /gene="qnlA"
     CDS             8200..9075
                     /gene="qnlA"
                     /note="QnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="L-QuiNAc synthase"
                     /protein_id="AAR24275.1"
                     /translation="MKILVIGATGMLGGSLLRYFADKTEHDVYGTVRDSNAESRLVSK
                     ANANIICGIDVHNVNKIRSIIEEVRPDYVINCVGVVKQLKESKYPIHSITINSLLPHR
                     LAEICSHNNSKLIHFSTDCVFSGNRGNYSVNDVPDAFDLYGRSKLLGEVSYAPHLTLR
                     TSIIGHEQGSQHSLIDWFLNQSGEVKGFTKAVFSGVPTVYMAELLNNYVFTNPDITGL
                     YQVSVEPIDKYSLLSLVKDIYGKDIDINADDSLVIDRSLNSTEFKIRTGMNNPSWKDL
                     IEKMYNEYRTYFQKA"
     gene            9044..10189
                     /gene="qnlB"
     CDS             9044..10189
                     /gene="qnlB"
                     /note="QnlB"
                     /codon_start=1
                     /transl_table=11
                     /product="L-QuiNAc synthase"
                     /protein_id="AAR24276.1"
                     /translation="MNIEHISKKLKVITFVGTRPEIIRLSRIIALLDKYCDHILVHTG
                     QNYDYELNEVFFSDLGIRKPDYFLNAAGKNAAETIGQIIIKSDSILEQVNPQALLILG
                     DTNSALVAISAKRRKIPIFHMEAGNRCFDFRVPEEINRKLVDHISDINLTYSHIARDY
                     LLREGIPADQVIKTGSPMREVLNYYRDDIQKSVVLETLKLSNNNYFVVSSHREENVDS
                     PERLRQLLEILNELAERYNLPVIVSTHPRTRKRFEDLSFNVHPNISFMKPFGFIDYIT
                     LQLHARAVLSDSGTITEESSILNFPALNLREVHERPEGFEEASVMFVGLNKERVLQAL
                     DILNEQPRYESRLLNLVEDYKPDNVSEKVLRIIMSYTDFVNNKVWKK"
     gene            10193..11410
                     /gene="wbwH"
     CDS             10193..11410
                     /gene="wbwH"
                     /note="WbwH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAR24277.1"
                     /translation="MRIALICDDYLPGSTRVSAKMMHELARELLQRGHEPIVVTPDCY
                     IKSIYKVEQLDGVDILRFRNGRIKDVSKIKRAMCETLLSYNGWKAVNSYFSNDVIDAV
                     IYYSPSIFFGPLVNKIKRKWSCPSFLILRDSFPQWVIDEGLIREGSLICRYFRFFEKI
                     NYQAADTIGVMSERNRELFLNMHPNIQSVEVLYNWCDTRADAELDLVNKPDCITKIAD
                     KIIFLYGGNIGKAQDMGNLIRLAIAMKTYSNVHFIFIGQGDEYDFVSDSIKKYSLSNV
                     SLFPSVSQHDFKVILKFVHVGLFSLSKKHTAHNFPGKLLGYMKNSLPILGSVNSGNDL
                     SDVINNNEAGCVCDNGDDNALLRAAIRLATDQDYRYMCGQKANQLLHEKFSVTSAIDN
                     IMKHLSKKTQDYN"
     CDS             11412..11930
                     /note="Orf10"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="AAR24278.1"
                     /translation="MTFKLLDFNLNNELKANAKKSERLRYHLSLHGSYNEPVQRIIIS
                     LMRGTYIPPHYHEFEYQWEYFNVISGNICVIIFDQNGTVTDKFKLGQNTGAYGVEFSS
                     NTIHTILCESEDSIILELKEGPFFPSKAKVIPSWAPDEQYCTYSRAEIVSVLEKIKPG
                     QNLPEALLAQEQ"
     gene            11935..13056
                     /gene="wbwI"
     CDS             11935..13056
                     /gene="wbwI"
                     /note="WbwI"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase"
                     /protein_id="AAR24279.1"
                     /translation="MEKKRFFALDSFRGLCALCVVIFHMKILNAFSEWDFFRNSRLFV
                     EFFFILSGFVLTHSYLKKETNGFYKYAIARTFRIFPLHIFMLCVYVILESFKLVAQQK
                     GFVFNTPPFQGGNSLKEFLPNLLLIQSWSSSFEYLSFNYPSWSISVEYYLYFIFYLVI
                     CPRTIIGRVFAGIVFFIIFLFQEYSSFKIFTSEVIRGGICFGAGILVYYLYSLIHLSH
                     ATRFLSVVFTILESSSLALIWFVVSMTDYESELLVVVVFSSSVFLFAFELGMISFFLK
                     KKPFQFFGKLSYSIYLTHASVLFVLISAMMVLQKITGVAFTQHIDNLRYLNFGSSLIN
                     NFMIFAVLGCVIFFSMLTYKFIELPAQRKGKELYEHYSK"
     gene            13165..>14504
                     /gene="gnd"
     CDS             13165..>14504
                     /gene="gnd"
                     /note="Gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="AAR24280.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSCEKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVHRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPY
                     FKKIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRD"
BASE COUNT     4441 a   2281 c   2942 g   4840 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtgactc atgcgtccaa gaacgcagtc
       61 gaaaaccact tcgacacttc ttatgaacta gaatctctcc ttgaactgcg cgtaaaacgc
      121 cagttgctgg cagaggttca gtccatctgc ccaccgggcg ttaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attctgtgtg ccagacctgc cattggcgac
      241 aatccattcg ttgtggtgct gccggatgtc gttattgatg acgccagcgc cgatccgctg
      301 cgttacaacc ttgccgccat gattgcgcga ttcaacgaaa cgggtcgtag ccaggttctg
      361 gcaaaacgta tgccgggtga cctttctgaa tactctgtca tccagactaa agaaccgctg
      421 gatcgtgaag gcaaagttag tcgcattgtt gaatttatcg aaaaacccga tcagccgcag
      481 acgctggact cagacattat ggcggttggt cgctatgtgc tttctgcgga catttggcct
      541 gaactggagc ggactcaacc cggtgcatgg ggacgtattc agctaactga tgcaattgct
      601 gaactggcga aaaaacagtc cgttgacgca atgttaatga cgggtgacag ctacgattgc
      661 ggtaagaaaa tgggctacat gcaggcattt gtgaagtacg gactacgcaa cctgaaagaa
      721 ggagcgaagt tccgcaaagg gattgagaag ctgttgttag aataagatat tgcgcgacgg
      781 gatagaatgt tggtgatgta acagaacggc agatgattgt ggttgaggaa gagctcactt
      841 cacagtaact gccgtatttg ttgtccctaa actacctttg gggaggtggt gattaatcct
      901 gtaaggatat attctgaata atgttcactt accacaagta aaaggggaca aaccgacaga
      961 ggaatttaca gaagttcatc aaatatgcat tgcgttgtaa atatcacata gtctggacgc
     1021 caaagtaccg cttcaggagc cagcaggata aattgggtaa agagctgtac agagccacct
     1081 atattttgtg cagaataaaa gattgcgagg ttcaggagtt aaatatacaa ccagatcatg
     1141 tgcatcttgt agcaatagtg cttctaaaaa tctcaatata tactctaatg gggcacctga
     1201 aggtcgcagt gcaatcaggc tctacaatcg gttttcgcat atcaggaaga aactatggaa
     1261 atcatttttg gccatggggc tgtgttgccg atatggtagg cgtatcagac gatatgtgat
     1321 acatcaggaa aagatagaac aaacatatga acagtaaatg gtgttgctag agtagaacgc
     1381 gggatatgac ggttgccccc ttatcggaga gtattctgaa aagctacctc atcaggagat
     1441 gatcttttat tattaaaaac aaccactaaa attagtgatt gcatggcgtt ttttgacaaa
     1501 aatgtcggat ttttccttgt tttataaagg gattgtgctc aaaatagtgg gctgaattgg
     1561 aagggagggt tcctcgcaaa cgcgaaggtt ggcgggagtt ggcttgcgac acacagaatt
     1621 ttgtgcagtg cactggtagc tgttgagcca ggggcggtag cgtttctgat gtgtgtgatt
     1681 tttacccatt taaaatgttc agcggaaaaa ttatgaagag tgccattagg agtggtaaag
     1741 agcaattttt aatgggagtc atctataaag cattatttat gttgataaca tcaataaatg
     1801 ctttagtaat agtaaagcta ctgactcctg aagagttagg tatttggtat atttttatgg
     1861 cgcttcaaac gcttttattt atcttaaata gcgccttgac tccgaatatt tcaagacagt
     1921 acagcattgc atttacggat attggtgagg gatttgatcc ccgtcttttt cacacaaaag
     1981 taattattat ttataataag ataattttag tggcaacagt catgggagtg ttttttactc
     2041 tgacttatct taactatgtg cttgatatat ttaggttggc taatgtttct atattactat
     2101 cttgggtaat tgtttttgct tccattttga tggaaattta ttttacatcg tatgagtgtg
     2161 cattaaatgg gtttggtgaa ttccaaaaag ttaacaaagt aaattttata tcgagattga
     2221 ttctaacgtt aagcattttt acactgttgt tatctgatgt gcatagtaat ggcttgctaa
     2281 tattctgtgg gttatatttt tttagtaata taataaaaag ggttatggtt attaatcttt
     2341 ttaatcatac ctttaaagac aaagggctaa aagaatcgcg gaaaacaata gatttttttg
     2401 atagaatcag ctgtaagcta caaaaactgt cctttttatc actactttca tcaattgggg
     2461 gggtcctcat tgtcagaagc ggaatgttta ttttgccctt ctatgtgcca atggaccaag
     2521 tagcatctta tggattaacc tatcagcttt ttgagctcgg gtttaatgtc atatttacta
     2581 tttctataat agcaactccc gcatggatct cagcctataa aaataatcca gaaggattaa
     2641 aaaaatcctt cacttgggta aagtatatat ctctattgct aatgttaatt tgtgggctgt
     2701 taattacatt tctgggtaat atcatattag agtttgctga tgttgaaacc agagtgcagt
     2761 cagttatcct ttgtttttat ttgctggtaa tttttgtgtt gcaaattaat cacagtattt
     2821 ctgggcaact cctaactatt cagaataaaa ttccttttgc ttatgcatca ttcttgagtg
     2881 ggatttttac attagtgtta tcggttgttt taattaaata ttattcagtt cttggcgcta
     2941 tttatgcgat attaatcagt cagctaatat ataataattg gaagtggcca tatgaagcat
     3001 ataaattctt gagggtgcat ggaaatgtat gagggaaaat taaaaaacaa aatattaagt
     3061 cttttcctct tatgtgaaac cttttgcttt atatatataa tatataataa ggaattagtt
     3121 tccgattatt tggggagggc gataacttta agtgagttta caataagttg tatttttttt
     3181 ctgctattgt tattttattt tttgttccat actttgtggg tgatttcaga aaaaataatt
     3241 aaatccagga aaaagattgg tagcgtcggg aatttgaatc tttttgttct tgttcttata
     3301 ttcttatatt ttatattaaa tttaaaaaca ggtattggta aagttggtca agaggataat
     3361 attactttaa caggattaga taaattatta tttgcagcac caatattttt taaaataaat
     3421 tttctcatgt atatttacgc tgcggggaat agaaataaga atcgtctgta ttactttaat
     3481 ttgttctttt tcgcatttgc agaattaact cggggagtat cttttacaat attgttgttg
     3541 ttgataatag aatacaataa gattagacgc ttcatttccc ttaggtattt tttattttca
     3601 ttgctttttg gtatattgtt aattaatgtg atttacaatg tgaaatttta cgttaggatg
     3661 ggggcgaaat acgagtatat tgatatctac acgtctttaa ttatgttgat gggacgtttg
     3721 agtatattgt ccaatttact atttattcat gataatctct ctgatataag attatatttt
     3781 tatggctcag ggtaccaagg agttgttcat gagtttttag agtcgttgac acctgtacca
     3841 tcattattcg gtattaacga gaaagtgttg gagtttggta aaatattatt ctcatattcg
     3901 aatgttactt ttaccagcgc aactgcagca tcgctattag gtatcattta tatttatcca
     3961 aattcaattt tcaatgcgat tacaataata tgtcttagtt ggatttatat acatataatt
     4021 acgggttatc ttgagtttag tcgaatgcaa cgagtagtcg cgtacttttt tgtgctgctt
     4081 gcactttacc agggcttcat ggccatatta agtaattata tatatgcatt aacaatatat
     4141 ttccttatag cctgtgccag aaatctttta ttgagtaaga aatatgaaac aatttagtat
     4201 taaaatcttg tcattggcag atgctatcga gcgtaggagg aaagtatcta gagagtttga
     4261 gctgaaatca gtactgccat tctcattttt taacggtatt tatggtaaga cactaagtaa
     4321 agaaaaatta gatacaatat actcttcaga gctggcgcag aaagtactcc atcggcaact
     4381 aactgcgggg gaaattggtg caacctactc acattacttg attttccgtg aggcatatga
     4441 gcgtggggat gagtttgttg ttgttctgga agatgactgt tatatagata gaaattttga
     4501 taccgtgtta acatcaattc ttgataagaa aaatcctaca gatgatgaaa ttatctttat
     4561 tcaaagacat acgagcgaaa actcacatat aataagatct ttaggcagtg agaagataaa
     4621 taaaaattat accttacatc gtatgctagg tagcgcgcag tattttgttg gtgcatacgg
     4681 gtatatcgta actagagctg caatgaaaaa aatgatcgat acatacttgc caatatattg
     4741 tgtatgtgat cattggtatt ttataaagaa gaaatgtcag atagaaaaat ttagtgtatt
     4801 atccccagct attgttttca caaatgatga ggatgttagg aaaatagata gttttgtaga
     4861 cattgaaaga agagaaatcg ctgttttcaa atcagtaggt aaaatagcaa aactaaaaat
     4921 tttaattaag aaaattattt taccaatatt aaataaagac tgggaataaa agtgctgcgc
     4981 tattataaag tattgactgt tctgcttaga ttaattatag cttttatatt gtaccctatt
     5041 gcatatgttt ttttgaatca caaaaaatat ttcatcattg gcacacgcaa tggtgttcga
     5101 gggcatgata atgctgagta ttttttgtct tattgtacaa gacacaatat taatacaaaa
     5161 gtaatttata atgatgctta tcagaaagat gaattatgca aaaactctat aaaaagtata
     5221 ttgtatatct tatgtgcaaa caatgtttac ataacgcact ctgaatcaga tgttttagat
     5281 tatttttggc gttttattcc cggtataaag tatatattta tacagcatgg agttattgga
     5341 ataaaaaaac ttccggatta tgaaaagaaa agatatcata gatatgtttc tagtagtgaa
     5401 tatgaaacag aaatttttag gaatttcttc catttgaaca gtgataaaat tattaaatct
     5461 ggacttccac gttttgattt ctatgactat aaccgttcaa aaaatgatga aattaaatcc
     5521 tgcttaatta tgtttacctg gagaaaatcg gattctggca aggctaaatt aattaaaaaa
     5581 tatactgaga tggttacttg cctgtcaaat gaagctctca ataaaattta catttgtgtt
     5641 catgaagcaa acttaagtac atttaaggtc ttgcttgaag aatcgcttga attggatgat
     5701 aggttttcat ttatagaaaa tgataaatta tcatgtatta ttcaaagtac agaactttta
     5761 atcactgact actcgagtgt tgcatgggat tatttatatc agaataaata tattttattt
     5821 tataccccag atattgatga ttataaaaat acaactggat tgtattgtga tttttccgat
     5881 ttttttggcg cacatcttat gaatttcagt agaatcaatt atccagtttt ggcatggatt
     5941 atgcaagaaa atgaaattaa aaataaaaac tttcttgcaa ggtataaatt ttataacatg
     6001 catgcaggca tacactctga gtatatagtg aagaatagtt aaaattatga gggcgaacat
     6061 tgacaactat tattgtctca gcaactgcat tggctaagag cggtgcatta acaatattaa
     6121 atgagtttat tgagtatgtg tcaaatttaa aaaaatataa gtttataatt tttattcctg
     6181 atagcattaa tttgcctaaa gcagttaata tcagatatat ccctgtgcct aaaaaaaatt
     6241 ggctatctag gatatattgg gatagttatg gcttaagaaa atatattcga gtaaatcggc
     6301 ttcaatatca ggcggtcatt tctcttcaga atacatctgt aaatgtagaa ggaaaacaga
     6361 taatatatct tcaccaatct ataccattta tagattttga tattccactg acaagtttat
     6421 ttaatttaaa attatggcta tacaagcgat tttattctta ttttatattt ttatttgtta
     6481 acaagaatac ttcttttatt gtccaagcca aatggctgaa agagctcctt tctgataagt
     6541 atcatatcga tgcaaaaaaa atatctatca taaagcccaa agcaagatat gttcctctag
     6601 caactaactc tattcataat gtagccaact tatctaagca attctgtaat attatttatc
     6661 cagcaacacc aatattttat aaaaatcatt gtgttattat tgatgctctt aggattttaa
     6721 gagaacagcg caatattgat aacttatgtt ttaatgtcac cttttcaaaa ggtgagtatg
     6781 ctaagtttga cgatttggtt aggcgttata acttgggtaa gaatattcgc tatttgggat
     6841 atttgacccg agatgaatta tatcaacatt atgataatag cgcttttatg gtctacccca
     6901 gttatgtgga aacctgtggg cttccgttgc tggaggctgc ttctaagcag ttgccaataa
     6961 tcgctagtga tctgccttat gcatgtgaaa tgcttgaggc ttatactggc gtcgtttatg
     7021 ttaagttcaa tgtctctacc gaatgggctc gagaaattga tacgatggcc cgagctggtg
     7081 aaactcgaat tattccgccg ttattaaaag cggagaatga ttctgattgg aataagttag
     7141 ataatattat tgaaggaaaa gatgatgttt aaaaataaaa cactactaat tacaggtgga
     7201 actggatcgt ttggtaatgc tgtgctccgt cgttttttat cgacggatat tggcgaaata
     7261 aggatcttta gtcgcgatga aaagaaacaa gatgatatgc gtaaaaaata ttcggatccg
     7321 aagttgaagt tttatattgg cgacgtaagg gattataaca gtattttgtc tgcaactcgc
     7381 ggagttgatt atatctatca tgccgcagca ttgaagcagg ttccttcctg cgagttttat
     7441 ccaatggaag cagtaaaaac taacgttatt ggtacagata atgtgttaga agctgcaata
     7501 gcgaataaag ttcagcgtat agtctgtttg agtacggata aagccgtata tcctatcaat
     7561 gcaatgggaa catcaaaagc aatgatggaa aaagtaattg ttgcaaaatc acgcaatttg
     7621 cctgaaggca taactatttg tgctacccgt tatggaaatg taatggcttc gcgtggatcg
     7681 gtgataccat tatttataaa tcaaattaaa aatggtaacc ctataactat taccgatccc
     7741 gatatgactc gctttatgat gacccttgat gatgccgtcg atttagtatt acatgcgttc
     7801 gaatacggaa ataatggtga tattttcgta caaaaggctc ctgcggcaac aattgaaaca
     7861 cttactaaag caattcaaca ggttacaaac gcacttgatc atcagataaa tattatcggg
     7921 acacgtcatg gtgaaaaatt gtatgaagtt ctctgtagcc gtgaagaaat ggctgtagca
     7981 gaagatcagg gaaattacta ccgtatccct gctgacaatc gagatttaaa ctatgagaaa
     8041 tactttgaga agggtaacaa agatgtttcc ttactcgagg attataattc gcataatact
     8101 attagacttg acgttgatgg tatggttgct ttgctgcgta aactcgaatt tatccgcaaa
     8161 gtggaagctg gttatgatgc aaatccagat gagtaaagca tgaaaatact tgttataggt
     8221 gctacaggaa tgctcggagg aagtttgctt cgttatttcg ctgacaagac cgagcatgat
     8281 gtatatggaa ctgttcgtga tagtaacgct gaaagtaggc tagtctcgaa agctaacgcc
     8341 aatataatct gcggtattga tgttcacaat gtgaataaaa tacgcagcat aatagaggaa
     8401 gtaagacctg actatgtaat taattgtgtt ggtgtagtta agcagcttaa agagtctaaa
     8461 tatcctattc attctattac tataaattca ttgctgcccc atcgattagc tgagatctgt
     8521 tcgcataata attcgaaatt aattcatttc tctacagatt gtgtattctc tggtaataga
     8581 ggtaattatt cggttaatga tgttcctgat gcctttgatc tttatggacg ttctaaatta
     8641 cttggtgaag taagttatgc tccacacctt acgttaagaa catcaataat tgggcatgag
     8701 caggggtcac agcatagttt aattgattgg tttttaaatc aatcaggtga ggtaaaagga
     8761 tttactaaag ctgtattctc cggagttcct actgtctata tggcggagct attaaataat
     8821 tatgttttta ctaatcctga tatcacaggg ttgtatcagg tgagtgttga acctattgat
     8881 aagtattctc ttctttcatt agttaaagat atttatggta aagatataga tattaatgcg
     8941 gatgatagtc ttgttattga cagaagtttg aactcaactg aatttaaaat tcgtacgggg
     9001 atgaataatc cctcttggaa agatttgata gagaagatgt acaatgaata tagaacatat
     9061 ttccaaaaag cttaaagtta ttacttttgt tggtacccgt cctgaaataa ttcgattgtc
     9121 tagaataatt gctttgttgg ataaatattg tgaccatatt ttggttcata ctggacaaaa
     9181 ttatgattat gagttgaatg aggttttttt ctccgattta ggaattagaa agcccgatta
     9241 ctttttgaat gctgcgggta aaaatgcggc agagactatt gggcaaatta ttattaaatc
     9301 tgatagtatt ctggaacaag ttaatccgca ggcactatta attttaggtg ataccaatag
     9361 tgccttggtt gcaatatctg caaaaagaag aaaaatccct attttccata tggaagcagg
     9421 gaatcgttgt tttgatttta gggttcctga agaaatcaat agaaaattgg ttgatcatat
     9481 ttctgatatt aatctgacct atagtcatat agctcgtgat tatttactac gtgaaggcat
     9541 tcccgctgat caggtgatca aaacgggatc tccgatgcgt gaagttttga attactatcg
     9601 tgatgatatc caaaagtcgg ttgtacttga aacgttgaaa cttagtaata acaactattt
     9661 tgttgtcagc tcccatcgcg aagagaatgt tgattcgcca gagagactta gacaactgct
     9721 ggagatcctc aatgagcttg ctgaaagata taatttgcct gtcattgttt caactcatcc
     9781 aagaaccaga aaaagattcg aagacttaag ttttaatgtt caccctaata tctctttcat
     9841 gaagccattc ggatttattg actacatcac tttacagttg catgccagag ctgtactttc
     9901 agatagtggt actattactg aagaatcttc tattcttaac ttcccagcac ttaatttacg
     9961 tgaggttcat gaacgtcctg aaggtttcga agaagcctca gtgatgtttg tcggtctgaa
    10021 taaagagcga gtattacagg ctcttgacat attaaatgag caaccaagat acgaatcacg
    10081 cctcttgaac cttgtagagg actacaaacc cgataacgta tcagagaaag ttctcaggat
    10141 tattatgagc tatactgatt ttgttaataa taaagtatgg aagaaataat cgatgcgcat
    10201 tgcgttaatt tgtgatgact atctccccgg tagtactcga gttagtgcaa aaatgatgca
    10261 tgaattggct cgtgaacttc tgcaaagagg tcatgagcca attgttgtga ctccggattg
    10321 ttacattaag tcaatttaca aagttgagca actggatggc gttgatatct tacgttttcg
    10381 taacggaaga atcaaagatg tgtccaaaat taaacgtgca atgtgtgaaa cactactctc
    10441 atataatggt tggaaggcgg taaatagtta tttttccaat gatgtgattg atgctgtcat
    10501 atattactca ccttctatat tctttggtcc tctggttaat aaaattaaga ggaaatggtc
    10561 atgtccatct tttcttatct tacgtgacag ttttcctcag tgggtaatcg atgaaggatt
    10621 aattagagag ggttctctga tatgtagata ttttagattt tttgagaaaa taaactatca
    10681 agcagcagat actattggag ttatgtcgga gaggaataga gaactttttt taaatatgca
    10741 tccaaatatt cagagtgttg aagtcttata taactggtgc gatactaggg cggatgcaga
    10801 actggacttg gtgaataaac ctgactgtat tactaagatc gcagataaaa ttatttttct
    10861 ctatggtgga aatataggca aggcacagga tatgggaaat ttaatccgtc ttgcaattgc
    10921 tatgaaaacc tactcaaatg ttcatttcat ttttattggg cagggtgatg aatacgattt
    10981 tgtaagtgat agtataaaaa aatattcact gagcaacgtt agcttgtttc catctgtttc
    11041 acaacatgat tttaaagtta ttctcaaatt tgttcatgtt gggttattta gtttgtctaa
    11101 gaaacatacc gcccataatt tccctggcaa gttacttggc tatatgaaaa atagtttacc
    11161 catactaggt agtgtaaata gtggtaatga tctgtcagat gttattaata ataatgaggc
    11221 gggttgtgtt tgtgacaatg gcgatgataa tgcattatta cgtgcggcaa taaggttagc
    11281 aactgatcag gattatcgtt acatgtgtgg acaaaaggct aatcagctac tacatgagaa
    11341 attttctgtt acttctgcta ttgacaatat aatgaagcac ttaagtaaaa aaactcagga
    11401 ctataattaa catgaccttt aaattattgg actttaatct aaataatgag ttgaaagcta
    11461 atgcaaagaa gagtgagcgt ttgcgatatc acttgagctt gcatggctct tataacgaac
    11521 ccgttcagag aattattata tcgctaatgc gtggtacata tattcctcca cactaccatg
    11581 aattcgagta tcaatgggag tattttaatg ttatatcagg taatatatgt gtgattattt
    11641 ttgatcagaa tggtactgtg actgataagt ttaaattggg tcaaaatact ggtgcttacg
    11701 gagttgagtt ttcttcaaac acaattcata caattctctg tgagagtgaa gatagtatta
    11761 ttttagagtt aaaagaaggt cctttttttc caagtaaagc taaagttata ccgagttggg
    11821 cgcctgatga gcaatattgc acatactctc gagcagaaat agtaagtgtt ttagaaaaaa
    11881 tcaaaccagg ccaaaatctt cctgaagcat tattggcgca ggaacaataa tgctatggaa
    11941 aaaaaacgat tttttgcact tgatagtttt cgtggtctat gcgctttgtg tgtagtcatt
    12001 tttcacatga aaatattgaa tgctttttcc gagtgggatt tttttcgaaa tagtcgactg
    12061 tttgttgaat ttttctttat tcttagtggg tttgttttaa cccacagcta tttaaaaaaa
    12121 gaaactaatg gtttttacaa atacgctatc gctcgaacgt ttcgaatttt tccacttcac
    12181 atttttatgc tttgtgtgta tgttattctt gaatctttta aattagttgc acaacagaaa
    12241 ggttttgtct ttaatacacc tccttttcag ggaggaaatt cattaaaaga atttcttcct
    12301 aatctgcttt taattcagtc gtggtcatcg agttttgaat acttaagctt caattatcca
    12361 tcatggagta taagtgttga atattattta tattttattt tttatctggt gatatgtcca
    12421 cgaacgataa ttggtagagt ttttgctggc atagtgtttt ttattatatt tctttttcaa
    12481 gaatattcct cttttaagat ttttaccagc gaagtgatta gaggtggaat ttgctttggc
    12541 gctggaattt tagtttatta tctttattca ttaattcatc taagtcatgc aactcgattt
    12601 ttatctgtgg tttttactat actagagtct agttctcttg cattaatttg gtttgttgta
    12661 agtatgactg attatgaaag tgaattattg gtggtggttg tcttttcgtc atcagttttt
    12721 ctatttgcat ttgaattagg gatgatttct ttttttctga aaaaaaaacc atttcagttt
    12781 tttggtaaac tatcttactc tatatatctt actcatgctt cagttctttt tgttcttata
    12841 tcagcaatga tggtattaca aaagataacg ggggtggcat ttacacagca tattgataat
    12901 ttacgttatc ttaattttgg aagttcattg ataaataatt ttatgatttt tgcagtgctt
    12961 ggatgtgtaa tatttttttc gatgctaacc tataaattta ttgagttgcc tgcgcaaaga
    13021 aagggtaaag agttatatga gcattattct aaataattgc tttaaagtta atgtgtcatt
    13081 ttatttctag actatgttac tttaacaaga ctaatttaat atattgtaca cacctaatcg
    13141 catcccccct gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca
    13201 gtgatggggc gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc
    13261 aaccgttcct gtgaaaagac ggaagaagtg attgccgaaa atccaggcaa gaaactggtt
    13321 ccttactata cggtgaaaga gtttgttgaa tctcttgaaa cgcctcgtcg tatcttgtta
    13381 atggtgaaag caggtgcagg cacggatgct gctattgatt cccttaagcc ataccttgaa
    13441 aaaggcgaca tcatcattga tggcggtaac accttcttcc aggacaccat tcgtcgtaac
    13501 cgtgagcttt ctgctgaagg ttttaacttc attggtacgg gggtttccgg tggtgaagag
    13561 ggcgcgctga aaggtccttc tatcatgcct ggtggtcaga aagaagccta tgaactggtt
    13621 gcaccgatcc tgaccaaaat tgctgccgta gctgaagatg gcgaaccgtg cgttacctat
    13681 attggtgcag acggtgctgg tcactatgtg aagatggttc acaacggtat tgaatacggt
    13741 gatatgcagt tgattgctga agcctattca ctgctgaaag gtggtctgaa tctctccaac
    13801 gaagaactgg cacaaacctt tactgagtgg aataacggtg aactgagcag ctacctgatc
    13861 gacatcacta aagacatttt caccaagaaa gatgaagacg gtaattatct ggttgacgtg
    13921 atcctggatg aagcggctaa taaaggcacc ggtaaatgga ccagtcagag tgcgctggat
    13981 ctcggcgagc cgctgtcgtt gattaccgag tctgtgtttg ctcgttatat ctcttctctg
    14041 aaagagcagc gtgttgctgc atctaaagtt ctgtctggtc ctcaggcaca gccagcaggc
    14101 gacaaagcgg agttcatcga gaaggttcac cgtgcgctgt atcttggcaa aatcgtttct
    14161 tatgcacagg gcttctctca actgcgtgcc gcgtctgaag agtacaactg ggatctgaac
    14221 tacggtgaaa tcgcgaagat tttccgtgct ggttgcatta ttcgtgcgca gttcttgcag
    14281 aaaatcaccg atgcctatgc cgaaaatcca aaaatcgcta acctgctgtt agctccgtat
    14341 ttcaagaaaa tcgccgatga ctaccagcag gcactgcgtg atgtcgtcgc ttatgcagtg
    14401 caaaacggta tcccagttcc aaccttctct gcggcggttg cttattacga cagctatcgt
    14461 gccgctgtcc tgcctgcgaa cctaatccag gcccagcgcg acta
//
