LOCUS       EU296412               10813 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella boydii type 15 O-antigen gene cluster, complete sequence.
ACCESSION   EU296412
VERSION     EU296412.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="type: 15"
     gene            1042..2433
                     /gene="wzx"
     CDS             1042..2433
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37077.1"
                     /translation="MNLLKGTIIYTLSNLTIKFGAVLLLPILTHLLNPEEYGMVGLYV
                     TLTSFLTIILGLGFYTPLMKTQSEQKSNIATSSHVNFCAIIFLIAVYLLLLLFLYFIL
                     KSSLVIDLLGEVKLDSKLIYLAVIVSMFSAINIIMNTSFRMDENYILVAILSILSFVL
                     FYSSAIIFIKQFNYGGLGYIYGNLLSSIIIFFISFTCYYRKLSIAFSWHNVKFLCGNG
                     IPMVFVELSDKIIEASDRFILVRYISLSSLGVYTLALTGCKVLNVVFNSYISAILPSI
                     YKSVESREDSNYIKVKLENAYVLVVMMVFLGQLISKEIIDIIFPASYSNLFFVFILAL
                     PAISLQYYYFLDFYFHRSEDSRFILCFTIATSIINIILNLIFVPKYGVYASLLSTYIS
                     YLIRTLVEIKFIARRYKLKFSVLKVLMGSFVLYTVPLLYHYGYYTVLLRVTLSIVLFI
                     GIIMFCKKSKIFN"
     misc_feature    1042..9417
                     /note="O-antigen gene cluster"
     gene            2453..3631
                     /gene="wzy"
     CDS             2453..3631
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD37078.1"
                     /translation="MVNAKNFVLIIFDVICLFLNVFVYVPRLIIGVLTVDKTLIITFP
                     LMLSVLAYNFIPPETYDLYRHYENYQNFSNNNEVSFIRDFFLYFLFTVGRLFDLNNGF
                     IAFVSCYILYFYWLKILYINFDYKANKKNFPLYFFIFFITPPLVIYTGVRFSTGLILT
                     LYGIVNWIQLRNVKLGIVYIICGLTAHFSMALIALIFIFTSYFNNIFSNKYLRVGLVF
                     SALLVISFNEQLVRFLTTVIEFINQLFDWNFISIDTYITGKWGLERAQELNSTGQLVL
                     GIRNYGMLFLILLFCPLISFNKNKYTNFYYVMCAVTILVFPFTAVFDRYGTFLIFFIV
                     YYLSTLQKKTNIEIIYFLMLIVYLIFFRTIDIKDNISVYTESYSNILNLSLIKVLLGI
                     "
     gene            3631..4611
                     /gene="wfeF"
     CDS             3631..4611
                     /gene="wfeF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37079.1"
                     /translation="MRKYENNIVISVIIPTYNNDKTIIEAIQSIVNVEMPVKYEVLVI
                     DDGSVDQTSNLIAEYISQRKDNTIKYFYQKNSGVSAARNVGIKNALGKYVLFLDADDT
                     YSEHVLKEIDEILSTNNDIYVFSYEKLNNKKAIISNKKNIITHYNNKYDFLNAYISGE
                     LFGCVSVCSCIFSKESLISNNIFYDSKIAYGEDQWFLINALKVCNDVEFRLELNLLNY
                     TYNTESATNRFNLRRFDAITMLERINQEAFKVNQDIINARINKELVNIATIYTKFNSL
                     QKSIMFVNNVILPKIKTDSDKRLYMFKDMVFTRLPYLYIILYKLYIQLFK"
     gene            4618..5799
                     /gene="wfeG"
     CDS             4618..5799
                     /gene="wfeG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37080.1"
                     /translation="MNILIIIPKLCNGGIEKIASNFSQYLPERYNQFVYSIMSQDSSY
                     DFAVKPIILNKSLGKTFIGKLITFFYRLHLARKIIRENNIDICISFGERCNIINILSM
                     GKTKKIITIHSQLSIENKTKGLYGKVTTLFSKLLYKNADATVAVSEIVKKDACGLLNL
                     DANNVEIIYNGHDIGYIKDKSTDYKEFDTPVIDFVSVGRITYAKGHYHLLRSPAIVKE
                     TYPNVILYIVGTYEKDNLKSIIDHLIEKYDLYDNVIFTGFSDNPYPYIKSAKALILSS
                     IFEGFPGVVIESIALGTPVIATDCGGASEVLRSPDAKIKNNTGDVEITKLGVLLPKFD
                     GIYDINLDFSPTEKKMAQVMNDIISGKLKFNEDFLITKANEYDIDSMMKKYTDLIEKI
                     K"
     gene            5799..6770
                     /gene="gae"
     CDS             5799..6770
                     /gene="gae"
                     /note="C5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gae"
                     /protein_id="ACD37081.1"
                     /translation="MKEATTSYKSIHTILEYFGWSHEDYWHINYKVNYDFSKFNYYWD
                     LTKKYNWFGGPFDQNNIPLYLGTDSKLHYSPIFLGHYALGAYQEYLLSNDKKAYDDFI
                     RIADWLVENTTTYKKCSGIWINTYPMSTFNLKGKWQSCLAQAKGISVLCRAYYLTQNL
                     KYLKVALDAFESFKIYKEEGGVKVRHKNVFFWEEYPTITNSIVLNGHIFAVWALYDLS
                     FVLKDKSEFKQQWADIYSYYKTSLIILKENYKLWDTHYWTRYDIWDKHFNVSSLFYHN
                     LHIKQFYILAVLGDDDDFKKIANKWAGKLKNPLFRMYALIKKVVFRL"
     gene            6767..7648
                     /gene="wfeH"
     CDS             6767..7648
                     /gene="wfeH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37082.1"
                     /translation="MIMNVIDKIIMGLKSIYLSFLYTIYNLFLNENRFEETNEVHVVV
                     SLTCFPGRIKKVYLTLESIMAQQYNNFKVVLYLSHEEFPKGLEDLPRSLIRLHKRGVD
                     INFTCENIRSYKKLHYALSDFPELPVITADDDVLYPSRWVNDFMESHKLFHDDILFAR
                     GHQITFDRNGNVKKYISFGKPAGYSASSLYIPTGVSGILYPPGCFFQDVQNKDIFMKL
                     APNADDIWYKVMTLLNGRKSRLIYKKPIHYPPILGTQKISLRKQNLSKQHLNNDTQLL
                     NLIDYYSIELADFKKKE"
     gene            7654..8394
                     /gene="wfeI"
     CDS             7654..8394
                     /gene="wfeI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37083.1"
                     /translation="MELVSIIMPCFNNEATIKESINSVLSQSYKYWELIIINDNSTDD
                     SQNIINDYVNSDERIKLITNEKNIGAGGSRNKGIEIANGRFIAFLDADDVWLPEKLAK
                     QIKFMRDGNYALTYSYYQKFDSTGLGAIVRSPATTTYRKLLYSNVIGCLTGMYDTDFL
                     GKCYMPLIRKRQDMGLWLSILKKCNKAYCLPEVLAYYRIDSGMTKNKLDAAKYQWRFY
                     RDVTKLNLIQSSWYFVWYSVLGLVKYKK"
     gene            8407..9417
                     /gene="gne"
     CDS             8407..9417
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACD37084.1"
                     /translation="MTILVTGGAGYIGSHTVIELQKNGFDVVIVDNFCNSSPSVLKRI
                     ELITGKEIKCYDIDLLDFNSLQKVFVTNNINSVIHFASLKSVSESLIKPTEYYHNNLS
                     GCLNVLKIMKEHNVNNFIFSSSATVYGSNNNNPVSESSSVGTATNPYGKSKIFLEQIL
                     SDCCKSNPNLNVTCLRYFNPVGAHSSGLIGESPNGVPANLVPYLTQVALGKLPVLHIY
                     GNDYDTKDGTGVRDFIHVTDLANGHIAALKKINGHNGFKVYNLGTGRGYSVLEVVRCF
                     EAITGKSIPIEFSPRREGDIAESWANVSAANYELEWFAKKTLTDMLRDAWKWQTLNPN
                     GL"
BASE COUNT     3567 a   1590 c   2047 g   3609 t
ORIGIN
        1 ctcctggtaa ctcatgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgaa
       61 ttagaatctc tccttgaaca gcgcgtgaag cgtcaactgc tggcggaagt acagtctatc
      121 tgtccgccgg gcgtgaccat tatgaacgtg cgtcagggcg aacctttagg tttgggccac
      181 tccattttgt gtgcacgacc cgccattggt gacaacccat ttgtcgtggt actgccggac
      241 gttgtgatcg acgacgccag tgccgacccg ctgcgctaca accttgctgc catgattgcg
      301 cgtttcaatg aaacgggccg tagccaggtg ctggcaaaac gtatgccggg tgacctttct
      361 gaatactccg ttattcagac caaagagccg ctggatcgtg aaggcaaagt cagccgcatt
      421 gttgaattta tcgaaaaacc ggatcagccg cagacgctgg actcagacat catggccgtt
      481 ggtcgctatg tgctttctgc cgatatttgg ccggaacttg aacgcactca acctggtgca
      541 tgggggcgta ttcagctgac tgatgccatt gctgaactag cgaaaaaaca gtccgttgat
      601 gcaatgctga tgactggtga cagctacgac tgcggtaaaa aaatgggcta tatgcaggcg
      661 tttgtgaagt atggcttacg caacctgaaa gaaggggcga agttccgtaa aggtattgag
      721 aaactgttaa gcgaataatg aaaatctgac cggatgtaac ggttgataag aaaattataa
      781 cggcggtgaa gattcctgtg gagagtaatt tgttgcgatt accactgtcg ttattcaact
      841 ataagcataa aaaagttaaa tataatttgg ggattttaag tatatggaaa cgcctttgtc
      901 tactgaataa gtaaggtatt aactgtaaga aaagcgtgaa tggtgttccc tacataattt
      961 aaagatgttc agtctactgg tagctgttaa gccaggggcg gtagcgtgga taattttgaa
     1021 aaaaaaagat tagagcataa aatgaattta cttaaaggga caataatata tacattgtca
     1081 aatttgacaa ttaaatttgg tgcagtttta ctgcttccta tattaactca tttacttaat
     1141 ccagaagagt atgggatggt aggattgtat gttacactta cttcctttct tacaataatt
     1201 ttagggcttg gtttttatac acccttaatg aaaactcagt cggagcagaa aagcaatatt
     1261 gcaacttcta gccatgtgaa tttttgtgca ataatatttc ttatcgcagt ttatttatta
     1321 ttgcttttat ttctttactt tatattgaag tcgtctttag ttattgattt gttgggagag
     1381 gtgaaactag atagcaaatt aatttatttg gcagttattg tatcaatgtt ttctgcaatt
     1441 aatattatta tgaacacgtc gtttagaatg gacgagaatt atatattagt tgcaatattg
     1501 tcaattttat cttttgtgtt attttattcc tctgctataa tattcattaa acagttcaat
     1561 tatggaggcc ttggctatat atatggcaat ttattaagtt caataatcat tttttttata
     1621 agtttcactt gttactatag aaaattatct attgcatttt catggcacaa cgtgaaattt
     1681 ttgtgtggga atggaattcc aatggttttt gtagagttat cggataaaat aattgaagcc
     1741 agcgatagat ttattttagt cagatatatt tcactatcct ctttaggcgt atatacacta
     1801 gcactgacag gatgtaaagt attaaacgta gttttcaatt cttatattag cgctatcctt
     1861 ccctctatct ataaaagcgt tgaaagccgt gaagattcaa attatataaa agttaagctt
     1921 gagaatgcat acgtgcttgt agtaatgatg gtttttttgg gacagttgat atcaaaagaa
     1981 ataattgaca taatttttcc tgcatcatat tcaaatttat tttttgtatt tatattagct
     2041 ttgcctgcaa tatctctaca atattattat tttttagatt tctattttca tagaagtgaa
     2101 gatagccgat ttattttatg ttttacaata gcaacctcga taataaatat tattctaaac
     2161 ttaatttttg ttcccaaata tggagtttat gcatctcttt tatcaacgta tatatcctat
     2221 ttaatcagaa cgctagtaga aataaaattt atagcgagac ggtataaatt gaagtttagt
     2281 gtgcttaaag tccttatggg aagctttgtt ctgtatactg ttcctttatt atatcattat
     2341 ggttattata cagtgttgtt gagagtaact ttgtcaattg ttttatttat aggtattatt
     2401 atgttttgta aaaaaagtaa aatattcaat taaaagtgag tgctaagtga tcatggttaa
     2461 tgctaaaaat tttgtattaa ttatttttga tgtaatatgt ctttttctta atgtttttgt
     2521 ttatgtccca cgtctaatta ttggtgtttt aacagtagat aaaacattga ttattacatt
     2581 tcccttgatg ttatcagtgt tggcatataa ttttattcca ccagaaacat atgatcttta
     2641 cagacattat gaaaactatc aaaatttttc gaataataat gaagtaagtt ttatcaggga
     2701 tttttttctt tattttttat ttaccgtagg gaggcttttt gatttaaata atggatttat
     2761 agcatttgta tcttgttata tcctctattt ttattggtta aaaatattat acattaactt
     2821 tgattataaa gccaacaaga aaaatttccc gttatacttt tttatatttt ttattactcc
     2881 acctcttgtt atatatacag gcgttcgatt ttccacaggg cttatattaa cactatatgg
     2941 gattgtaaac tggattcaat tacggaatgt aaaacttggt atagtatata ttatttgcgg
     3001 gttaacagcc catttttcaa tggcattgat tgctttaata tttattttca catcatattt
     3061 caataatatc ttttcaaata aatatttaag ggttgggctc gtattttctg cattacttgt
     3121 aatctcattt aatgaacagt tagtaaggtt tttgacaaca gtaattgagt tcattaatca
     3181 gttatttgat tggaacttta ttagtatcga tacatatatt actggtaaat gggggctgga
     3241 gagggcgcag gaattgaatt caaccggaca attagtatta ggtatacgta attatggtat
     3301 gctctttcta atattgctat tttgcccact aatatctttt aataaaaata aatatacgaa
     3361 cttttattat gtaatgtgtg ctgttacaat attagttttc ccttttacag cagtatttga
     3421 tagatatgga acatttttaa ttttttttat tgtatactat cttagtactc ttcagaaaaa
     3481 aactaacatt gaaataatat attttttaat gttgattgta tatttgattt tctttagaac
     3541 aatagacatc aaagataata tttccgtata cactgaaagc tatagtaata tattgaatct
     3601 ttctttaatt aaagtactgt taggaatata atgcgtaaat atgaaaataa tattgttata
     3661 agtgtgatta ttccaacata taacaatgat aaaacaatca tagaggctat tcaatctata
     3721 gtaaatgttg agatgcctgt gaaatatgag gtactagtaa tagacgatgg ttcggtagat
     3781 cagacatcaa atttaattgc agaatatata agtcaaagaa aagataatac tattaaatat
     3841 ttttatcaaa aaaacagtgg tgtaagcgct gcgagaaacg tgggaataaa aaatgcatta
     3901 gggaaatatg ttctattttt agacgcagat gatacatata gtgaacatgt gttaaaagaa
     3961 atagatgaaa tattaagtac taacaatgat atatatgtgt tttcatatga aaaactgaat
     4021 aataaaaagg ctataatatc aaataaaaaa aatattataa ctcattataa taataaatat
     4081 gattttttaa acgcctatat ctcaggtgaa cttttcgggt gcgttagtgt ttgctcttgc
     4141 atttttagta aagaatcatt aattagcaat aatatttttt acgatagtaa aattgcttat
     4201 ggggaagatc aatggttttt gataaatgct ctcaaggttt gcaatgatgt agagttcaga
     4261 ttagaactaa atttactgaa ttatacttat aatacggagt cggcgactaa tcgtttcaac
     4321 ctaagacgtt ttgacgcgat tacaatgcta gaaagaatca atcaagaagc atttaaggtt
     4381 aatcaagata taattaatgc aagaattaat aaagaattgg taaatatagc aacaatatat
     4441 acgaaattca attcgttaca gaaatcaata atgtttgtta ataacgttat actcccaaaa
     4501 ataaaaacgg atagtgacaa aagactatat atgttcaaag atatggtttt cactagatta
     4561 ccatatttat atataatact ttataaactc tatattcagc tatttaagta aactaatatg
     4621 aatattctta taattattcc taagttatgt aacggtggga ttgaaaagat tgcatcaaac
     4681 ttttctcaat acttaccaga aagatataat caatttgtat attcaatcat gtcacaagac
     4741 tcttcttatg attttgcggt aaagcccatt atattaaata aaagtttagg taaaaccttt
     4801 attggaaaat tgattacatt tttctacagg cttcatttgg cacgtaaaat tattagagaa
     4861 aataacatag atatatgcat tagttttggg gagagatgta atattataaa tattctatct
     4921 atggggaaaa caaaaaaaat cattactata catagtcagc tatctattga aaataaaacg
     4981 aaaggattat atggaaaagt aacaacacta ttttcaaaat tgctttataa aaatgcggat
     5041 gctactgtag ccgtatcaga aatagtgaag aaggatgcat gcggtttatt aaatttagat
     5101 gcgaataatg tagagataat ttataatgga cacgatattg gttatatcaa agataagtct
     5161 actgattata aagaatttga tacacctgtg atcgacttcg tgtctgtagg gcgaattaca
     5221 tatgctaaag ggcattatca tctattgaga agtcctgcca tagtaaagga gacgtatcct
     5281 aatgtaatac tatatattgt tggcacgtat gaaaaagata atttaaaaag cataattgat
     5341 catttaattg agaaatacga tttatatgat aatgtgattt ttaccggctt tagtgacaat
     5401 ccatatcctt acataaaatc agcaaaggct cttatactta gttcaatttt tgaaggtttt
     5461 ccaggcgttg ttatcgagag tattgcgctt ggaactcctg tcatagcgac agattgtggc
     5521 ggtgcttcag aagttttaag aagtccagat gccaaaataa agaataatac gggagatgtg
     5581 gaaattacta aattaggagt actgttacca aaattcgatg ggatttatga tattaatttg
     5641 gatttttcgc ccacagaaaa aaagatggcg caagttatga atgatattat atcagggaag
     5701 ctgaaattta atgaagactt tttaattaca aaagcaaacg aatatgatat agattctatg
     5761 atgaaaaaat atacggactt aatagagaaa ataaaataat gaaagaagct actacctcat
     5821 ataaatcaat tcatactata ttagaatatt ttggttggtc tcatgaagat tattggcata
     5881 tcaactataa agtaaattat gatttttcaa aatttaatta ttattgggat ttgacaaaaa
     5941 agtataattg gtttggtgga ccttttgatc aaaataatat tcctttatat cttggcactg
     6001 attcaaaact gcattactca ccaatttttt tagggcatta tgctcttgga gcatatcaag
     6061 aatatttatt atcaaatgat aaaaaagcat atgacgattt tataagaata gcggattggc
     6121 tggttgaaaa tacaacgaca tataaaaaat gttctggaat atggataaat acatacccaa
     6181 tgtcaacttt taacttaaaa ggtaaatggc aatcgtgttt agcccaggca aaaggtatat
     6241 ctgtcctatg tagagcttat tatcttacgc aaaatttaaa atatttgaag gtagctcttg
     6301 acgcatttga atctttcaaa atatataaag aagaaggtgg agttaaggta aggcataaaa
     6361 acgtattctt ttgggaggaa tatccaacta taaccaattc tatcgtattg aatggacata
     6421 tttttgcagt ttgggcattg tacgatttat cttttgtatt aaaagataag agcgagttta
     6481 agcaacaatg ggcggatatt tacagttatt ataaaacatc actgattata ttaaaagaga
     6541 attataagtt atgggatacg cactattgga ctagatatga tatatgggat aaacatttta
     6601 atgtttcaag tttgttttat cataatctcc atattaaaca attctatata ttggctgttt
     6661 tgggagatga tgatgatttc aagaaaattg ctaataaatg ggctggtaaa ttaaaaaatc
     6721 cgcttttcag aatgtatgcg cttataaaga aagtagtttt taggttatga ttatgaatgt
     6781 tattgataaa attattatgg gtttgaaaag tatatattta agctttcttt atacaatata
     6841 taatttattt cttaatgaaa atagattcga agagacaaat gaagtacatg ttgttgtaag
     6901 tttgacgtgt tttcctggac gtataaaaaa agtatatctt actttagagt caattatggc
     6961 tcaacaatat aataacttta aagtggtatt gtacttgtct catgaagagt ttccaaaggg
     7021 tttagaggat ttacctcgta gtttaatacg attacataaa agaggtgtcg atattaattt
     7081 tacgtgtgaa aatattcgat cttataagaa gttacattat gcgctgagtg atttcccaga
     7141 actacctgta ataacagctg atgatgatgt cttatatccc tcgcgatggg taaatgattt
     7201 tatggagtca cataagcttt ttcatgatga tatattattt gcgagggggc atcagataac
     7261 atttgaccga aatggaaatg ttaaaaaata tatctctttc ggaaaaccag ctggatattc
     7321 agcatcaagt ctatatattc ctacaggagt aagtggaatt ctatatccgc caggatgttt
     7381 ttttcaagat gtgcagaata aagatatttt tatgaagttg gcacctaacg cagatgatat
     7441 atggtataaa gtaatgactt tgttaaacgg tcgaaaatcc agattgatct ataaaaagcc
     7501 aattcattat ccacctatcc ttggcacgca gaagatctcc ttgcgtaaac aaaacttatc
     7561 gaaacaacat ttaaataatg atacacaatt attaaattta attgattatt attctatcga
     7621 attagctgat tttaagaaaa aggaatagct cctatggaat tagtttcaat tataatgcct
     7681 tgttttaata atgaagcaac tataaaagaa agtattaata gcgtactatc tcaaagttat
     7741 aaatactggg agttaattat tatcaatgac aactcaacag atgattcaca aaatattatt
     7801 aacgactatg tgaatagtga tgaaagaata aaactgatta ctaatgaaaa aaatatcgga
     7861 gctggaggta gtagaaataa aggaattgaa attgcaaatg gaagattcat tgctttttta
     7921 gatgcagatg atgtttggtt gccagaaaaa cttgcaaaac aaataaagtt tatgagagat
     7981 ggtaattatg cactgacata tagttattat caaaagtttg attcaactgg attgggagcg
     8041 atcgtccggt cacctgcgac aacaacttat aggaaattgt tatacagtaa tgttattgga
     8101 tgtctaacag gaatgtatga tactgatttt ttgggtaaat gctatatgcc tttaattcgt
     8161 aaacgacaag atatggggct ttggttgtca atattgaaaa agtgtaataa agcatattgc
     8221 ttacctgaag ttttggcata ttatagaatt gactcaggaa tgacaaaaaa taaacttgat
     8281 gcggcaaaat atcagtggcg attttatagg gatgtaacta aacttaattt gattcaatca
     8341 agttggtatt ttgtttggta ttcagtttta ggtttggtaa agtacaaaaa ataataagga
     8401 ggagtgatga caattttagt cactggtggt gctggatata ttggcagtca cactgttatt
     8461 gaattacaga aaaatggttt tgacgtggta atagtagata atttttgtaa ttcatcgcca
     8521 tcggttttaa aaagaattga attaattaca ggaaaggaaa ttaaatgtta cgatattgat
     8581 cttctggatt ttaattcttt gcagaaggtt tttgtaacta ataatattaa ctctgtgatt
     8641 cattttgcat ctttaaaatc agtttctgaa tcactaatca aaccaactga atattatcat
     8701 aataatttga gtggttgcct caatgtcttg aaaataatga aagaacataa cgtgaataat
     8761 ttcattttca gttcttcggc aactgtatat ggcagtaata acaacaatcc tgtttcagaa
     8821 tcttcatcag tcggcaccgc aacaaatcct tatggaaaat ccaagatttt tttagagcaa
     8881 atattgtctg attgctgtaa atcaaatcct aatttaaatg taacttgcct gaggtatttc
     8941 aatccggttg gtgcacattc ttcaggtttg ataggagaat ctccgaatgg tgttcctgcc
     9001 aatttagttc catatttaac tcaggttgct ttaggtaaat tgcccgtatt acatatatat
     9061 ggtaatgact atgataccaa ggatgggacg ggagtaagag attttattca tgttacggat
     9121 ttagctaatg gacacatagc tgcgctgaaa aaaataaatg gtcataatgg ttttaaagtt
     9181 tataatcttg gtactgggcg aggttatagt gtgttagaag tcgttagatg ttttgaggct
     9241 attacaggaa aaagtattcc aatcgaattt tcgcctcgca gagaaggtga tatcgctgaa
     9301 agttgggcaa atgtttccgc agcaaattat gagttagagt ggttcgctaa aaagacatta
     9361 actgatatgt tacgagatgc atggaaatgg caaacgttaa atccaaatgg attataaaga
     9421 tatttaattt atattaaacc ttgcttattt aagataatgt aaaccgcacc tttgcggtaa
     9481 ccacccctga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt
     9541 gatggggcgc aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa
     9601 ccgttcccgt gaaaagaccg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc
     9661 ttactatacg gtgaaagagt ttgttgaatc tttggaaacg cctcgtcgca tcctgttaat
     9721 ggtgaaagca ggtgcaggca cggatgctgc tattgattcc cttaagccat atctcgataa
     9781 aggcgacatc atcattgatg gcggtaacac cttcttccag gacaccattc gtcgtaaccg
     9841 tgagctttct gccgaaggct ttaacttcat tggtaccggt gtctccggtg gtgaagaagg
     9901 cgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg aactggttgc
     9961 tccgatcctg accaaaatcg ctgcagtggc tgaagacggc gagccatgcg ttacctatat
    10021 tggtgccgat ggtgcaggtc actatgtgaa gatggttcac aacggtattg aatacggcga
    10081 tatgcagctg attgccgaag cctattctct gctaaaaggt ggcctgaacc ttaccaacga
    10141 agaattggca cagaccttta ccgaatggaa taacggtgaa ctgagcagct acctgatcga
    10201 cattaccaaa gacatcttca ctaaaaaaga tgaagacggt aactacctgg ttgatgtgat
    10261 cctggatgaa gcggcaaaca aaggtacggg caaatggacc agccagagcg cactggatct
    10321 cggcgaaccg ctgtcgctga ttaccgagtc tgtgtttgca cgttatatct cttctctgaa
    10381 agatcagcgc gttgccgctt ctaaagttct ctctggcccg caagcgcagc cagcaggcga
    10441 caaggctgag ttcatcgaaa aagttcgtcg tgcgctgtat ctgggcaaaa tcgtttctta
    10501 cgctcagggc ttctctcagt tgcgtgctgc gtctgaagaa tacaactggg atctgaacta
    10561 cggcgaaatc gcgaagattt tccgtgctgg ctgcattatc cgtgcgcagt tcctgcagaa
    10621 aatcaccgat gcctatgccg aaaatccgca gatcgctaac ctgctgctgg ctccgtactt
    10681 caagcaaatt gccgatgact atcagcaggc gctgcgtgat gtcgttgctt atgcagtaca
    10741 gaacggtatc ccggttccga ccttcgccgc tgcggttgcc tattatgaca gctaccgtgc
    10801 cgctgttctg cct
//
