LOCUS       DQ371800               11616 bp    DNA     linear   BCT 06-SEP-2006
DEFINITION  Shigella boydii serotype 16 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ371800
VERSION     DQ371800.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Liu,B., Senchenkova,S.N., Feng,L., Perepelov,A.V., Xu,T.,
            Shevelev,S.D., Zhu,Y., Shashkov,A.S., Zou,M., Knirel,Y.A. and
            Wang,L.
  TITLE     Structural and molecular characterization of Shigella boydii type
            16 O antigen
  JOURNAL   Gene 380 (1), 46-53 (2006)
   PUBMED   16859842
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Liu,B., Senchenkova,S.N., Feng,L., Perepelov,A.V., Xu,T.,
            Shevelev,S.D., Shashkov,A.S., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R.China
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="16"
                     /db_xref="taxon:621"
     gene            372..1325
                     /gene="wfaS"
     CDS             372..1325
                     /gene="wfaX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABD19776.1"
                     /translation="MLFPVVLFVYNRADHTKQTIDALKNNILAPLTDLYIYSDFPKAN
                     SDIQAVKEVRCLLHNINGFKSVTIIERDRNYGLGKSVIQGVTEILKRHDAVIVLEDDL
                     VTHKDFLTYMNKMLELFHDDPEVFSISGYSLPNMRSIGTEDYYFVPRISSWGWGCWKD
                     RWEDLDWDIKKYKQLISDKRACQLFNCAGNDMLDMLIHQVEGEAESWAIRFDYNRFIK
                     GNLLTIYPRVSFIKNIGMDGSGVHGENTCKFDNEFDCKRFINVQGIEKKYNHDINPLV
                     ISEFKNVYKRKFKSIILIYLRRYAFYNYHKKIYKNVMGLLK"
     misc_feature    372..10083
                     /note="O antigen gene cluster"
     gene            1322..2395
                     /gene="wfaT"
     CDS             1322..2395
                     /gene="wfaT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABD19777.1"
                     /translation="MIKKKIAIAVPDITFVGGAEKVAINLANKLCDIYDVTVISLFQW
                     QNQNNFFLGNDNVKNISLGLEKCCSVFSRLLLNIRNRNKLKGIFNCYDYVIGNNFFRY
                     YVYPFKTNCKLCEIQHLRFEEEKGGDSFIRRLLYRKLDKLIVLTENDSAKFRRSGYVN
                     IETIPNFISDIKENISYNNKSKRIVAIGRLAYQKNYNDMLDIMFILKKKHPDFILDIL
                     GEGDEKTQIEKKIIDLKLSENVVLHGAVENVDDYLSNAALLISTSRYEGFPLSFLEAL
                     NFELPIITYDFDSGARDIVFHSENGILVNIGEKSVFANYLAEFLENENIRIKFSKKAR
                     YLKKLYSSEVVVKQWQTRIFNDA"
     gene            2385..3785
                     /gene="wzx"
     CDS             2385..3785
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ABD19778.1"
                     /translation="MMLRSGSLQFVFSIFLAIIQLIMLRLMHEYLNAFELGLYSLAMM
                     LIFLLQSFCDMGISSFSINSNNDNYYFNNKLHKLSLLLGFFSFVLGVLGTFILANIYN
                     ADVFIFHGFLLSLSFPFLTLTGYYQSIMVRKKKLVAIAIYDFLSRALSLILLYFMFVI
                     GGRFDAFIYSYLVYAVIRCCLYFVTSNNLVTFISFRVYPSEDGHDENISYRNFFSFMI
                     FQFLGQFVNSISVKVDEMVLGRVLGLELLGIYTLVKNYVVQVGYLVIPLVRRISLSIL
                     VEKSDQNNNYIKLSYFFSYLLVAYFLGGAIVSKLMLNIVFGNNISEYLPVFIIMLITW
                     AVRVAGGSVLSSYFVVKGKPNYDFYWNLLQGSIIACVAIFTEYKNLQDLAVHIFIAYS
                     LYVIIAAPVFCYLLKSEWSKSFFTNFVVISICGIIVINRNLLISLTITESMGVIFIYG
                     LLFCLLSYFFVRKHII"
     gene            3796..4953
                     /gene="wzy"
     CDS             3796..4953
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ABD19779.1"
                     /translation="MTIVTNRMLLFFIFLIINVPNVEVEYGGSILKIYNAIFFLLLLL
                     YIKKPQWKLHMISSYLLFIVLFFTAYLIINGRDEHLIAIANSYIPNEWQVLYGKQVFD
                     DFYRSTTVSLFQLANFLILVFYFNFFHNVNRSLFLFDSMKYVLAISILTQISISIIQL
                     VAFNNDRPSGSFGNAQSLGFFYLLCICYYSVFLPSWKKLISIIVLLLAIIITGTRSAL
                     FIAIIITALNMLNISVFMRKLILFLLFISVVILCSLFSSSDVFRGVILEIIGLFTSPH
                     SMYVRGLMWNSFYQTLTESPLFGTKGITVYFADNFFWFFILSYGICGAVFIGVFFLSI
                     IRSANSKFVFFLGVCTILQSVSYYGFFIENMGVVLTIFLAISLNHKVKIGY"
     gene            4956..6065
                     /gene="wfaU"
     CDS             4956..6065
                     /gene="wfaU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABD19780.1"
                     /translation="MRVTLLSINSLEENTGGGIYLRCLQKLYLKNGANVNVVCKSHNN
                     RYRKNVLTDLLSRIIFLSPSFLACYLFQVIRQCKDSDIIAIHSSRLGFYAWILKKIYP
                     HKKIYVHTDNVEFNLLKDNLLHKNIFRRILYNIDFFLIPMSEKMCVRWGDKVTFITRE
                     DAIDYKRMYALTYNERHILPIYLEPNDITKKNHSQRNKIIFTGSFDFYPNQEVVSRII
                     KIAEQTPTLQYIVAGRRLDIFIKNAGIQAPDNVTFESDIEPERLEQLYLESRLFLCPV
                     RHGSGMKTKIAEALSYGLYVIANTRSTCGYETAIEAGVISCVNDTTFIIDATSEIVDC
                     FSREDKDIIYKSLHVFNENYNLNNGVKYFSEILYK"
     gene            6062..7249
                     /gene="wfaV"
     CDS             6062..7249
                     /gene="wfaV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABD19781.1"
                     /translation="MKKILILTPRFPFPVIGGDRLRIYKICENLSKKYDLTLLSLCES
                     EKEMNMQIEDNVFKKVYRVKLSPLYSYLNVLLSIPTNIPLQIAYYKSTKFRKLIDELL
                     PEHDACIAHLIRVGDYIKDNKGIRILEMTDAISLNYKRVKINTNTNLRSIIYSIEQKR
                     LEHYERKMASHFDLVSFVSHIDKDFLYKDKNKGNVKVYSNGVDTTLLKFKRRHLKHDR
                     PIEIIFIGNMYSLQNMDAVIYFAHQILPYLNREGNYFNLKVIGKIREVDRRKLQLIKN
                     VTVTGIVNSVSEAAEDGHIGICPMRIGAGLQNKILEYMALGLPCITSTVGFEGTGATR
                     DREIFVADTLTEYKEIFEQLLSNHGLYENVALNARQFVETKFSWNAQLGSMIGDIDNL
                     LGE"
     gene            7251..8660
                     /gene="manC"
     CDS             7251..8660
                     /gene="manC"
                     /note="mannose-1-phosphate guanylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ABD19782.1"
                     /translation="MILPVIMAGGSGSRLWPLSRTHYPKQFHNLLGKSSLLIDTITRL
                     DNIKHQPPLVICNEDHRFLVAEQFRKHSLSTSGIILEPIGKNTCPAIALAAFQAVKTN
                     EDPYLLVLAADHSIGDRDSFQKAIHQAYETAKTGSLVTFGIIPTSPETGYGYIKKGKS
                     LNKYAYYVDGFKEKPNIILANEYINSGHYLWNSGMFLFKASSFLKELKKFEPKIYRLC
                     EESIDNSTHDLDFIRIDSDAFSNCKSQSVDFAIMEQTKNCAVVPMNADWSDVGSWSAL
                     WELSKKDDRNNYFYGDVVAIDCDSNFIRTDEKLTVTLGVKDLIVINTKDALLVAERSC
                     AQKVKDVVAKLNVDYRNEISHYSNEFRSWGEIEKVDSDNKYIVNRIIIKPGAKISKQI
                     HYHRSEHWIVVSGTASIVIDGKESVLTENESTFIKVGQEHSIMNPGLVPLIIIEIQSG
                     SYVSEDDIIRVKEIYNDEC"
     gene            8647..10083
                     /gene="manB"
     CDS             8647..10083
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ABD19783.1"
                     /translation="MMSANIVCQNIINVGKITFGTSGARGLVIDFSHDVCAAFTHAFL
                     SVIDDKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGYTVEYHGVIPTPALAHYSMQK
                     NIPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQG
                     ADYYLERYVSLFNPEILKGKRIGVYEHSSAGRDLYALLFNQLGAEVISLGRSDEFVPI
                     DTEAVSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIEL
                     NIKALAIPVSCNTAIEQSHKFASIQRTKIGSPYVIAAFADLAKQFNSVAGFEANGGFL
                     LASDLQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRLKNFPS
                     DSSQQIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPEL
                     RCYAEASDENQARQYVTNVLGNITSLIS"
BASE COUNT     3731 a   1757 c   2256 g   3872 t
ORIGIN
        1 cctgaaagaa ggggcgaagt tccgtaaagg tattgagaag ctgttaagcg aataatgaaa
       61 atctgaccga atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa
      121 agtaatttgc tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag
      181 ttagcaatag gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg
      241 taagacaatt agcgtttgaa tttttcgagc taagcgcgag tgggtaacgc tcgtcacatc
      301 gtaggcatgc atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgtgttaata
      361 aaggatcact aatgttattt ccagtagtat tatttgtata taatagagct gatcatacaa
      421 aacaaacaat agacgcgctt aagaataata tattagcacc tttaactgac ttatatatat
      481 atagtgattt tccaaaagca aattctgata ttcaagcagt taaagaagtg cgttgtttgt
      541 tgcataatat aaacgggttc aaaagtgtaa caataattga gcgcgataga aattatggtt
      601 taggtaagtc cgtgatccag ggcgtgaccg aaatactcaa aaggcatgat gctgttattg
      661 tattagaaga tgatttggtt acacataaag atttcctaac atatatgaat aaaatgttag
      721 agctatttca tgatgatcct gaggtgtttt ctatttcagg ttatagctta ccaaatatga
      781 gaagcattgg cactgaggat tattatttcg tgccacgtat ttcgtcatgg ggatgggggt
      841 gttggaaaga tcggtgggaa gatctagatt gggatataaa aaaatataaa caattaattt
      901 cagataaaag agcttgccaa ttgtttaatt gtgctggcaa tgatatgttg gacatgttga
      961 ttcatcaagt cgagggagag gcggaatcat gggctattag atttgattat aatagattta
     1021 taaaaggaaa tttgcttaca atttatccaa gagtgtcatt tataaaaaat attggtatgg
     1081 atggctctgg tgtccacggc gaaaatactt gtaagtttga taatgagttt gattgcaaga
     1141 gatttataaa cgtacaaggt atagaaaaaa aatataatca tgatataaat ccattggtta
     1201 tatctgaatt caaaaatgtc tataaaagaa aatttaaaag cataatcctt atttatctaa
     1261 gaagatatgc tttttataat taccataaaa aaatctataa aaatgtgatg gggcttttaa
     1321 agtgataaaa aagaaaatag caatagctgt tccagacatt acgtttgttg gaggggctga
     1381 aaaagtagca ataaatttgg caaacaaact ttgtgatata tatgacgtaa ccgtaataag
     1441 tttgtttcaa tggcaaaatc aaaataattt ttttcttgga aatgataatg taaaaaatat
     1501 atcattggga ttggagaaat gttgttcagt attttcaaga ctattactta atatacgaaa
     1561 tagaaataag ttgaagggaa tatttaattg ttatgattat gttataggta ataatttctt
     1621 tagatattac gtatatccgt ttaaaacaaa ttgcaaatta tgtgaaatac aacatttaag
     1681 atttgaggag gaaaaagggg gggactcttt tatacggcgt ttgttataca ggaaattaga
     1741 taaattgatt gttttaacag aaaatgattc agcaaagttt cgacgatctg gttatgtaaa
     1801 tatagaaact attccaaatt ttatctcaga tattaaagag aacatttcat acaacaataa
     1861 atcaaaaaga atcgttgcga ttggacgctt agcatatcaa aaaaactata atgatatgct
     1921 tgatatcatg tttattttaa aaaaaaaaca tccggatttt atattagaca tacttggaga
     1981 aggggatgaa aagacgcaaa ttgaaaaaaa aataatagat ctaaaattaa gcgaaaatgt
     2041 cgttttacat ggtgcagttg aaaatgtgga tgattatctt tcaaatgcag ctctattgat
     2101 ttcaacttct cgttatgaag gattcccgtt atctttttta gaggctttga atttcgaact
     2161 accaattata acgtacgact ttgattcagg tgctcgcgat atcgtatttc atagtgaaaa
     2221 tggaatacta gtgaatatag gggaaaagtc agtttttgca aattatttgg ctgaattctt
     2281 agaaaacgaa aatataagaa taaaatttag taagaaagct cgatatttaa aaaaattata
     2341 tagttcggaa gtagttgtga agcaatggca gacaaggatt tttaatgatg cttagatcag
     2401 gaagtttgca atttgttttt tcaatattct tagcaatcat acagttaata atgttaaggt
     2461 tgatgcatga gtacttaaat gcattcgaac ttggcttata ttcattagca atgatgctga
     2521 tatttttact tcagtcattt tgtgatatgg gcatatcatc attttcaatt aatagcaata
     2581 atgataatta ttattttaat aataaacttc ataagttatc attattactc ggtttttttt
     2641 catttgtctt gggagtatta ggaactttta tattagcaaa tatatacaat gccgatgtat
     2701 ttatctttca tggtttttta ttatctttat cctttccatt tttaaccttg acaggttatt
     2761 atcaatcaat tatggttagg aagaagaaat tagtagctat tgcaatttat gacttcttat
     2821 caagagcttt atcattaatt ttactatact ttatgtttgt catagggggg aggtttgatg
     2881 ctttcatata tagctatttg gtttatgcag taattagatg ctgtttatat tttgtgacat
     2941 caaataatct ggtaacgttt atttcattta gagtatatcc ttcagaagat ggacatgatg
     3001 aaaatatctc ctatagaaat tttttttcat tcatgatctt tcaattccta gggcagtttg
     3061 tgaactcaat ttctgtgaaa gttgacgaaa tggttttggg acgtgttctt gggctagaat
     3121 tattagggat ttatacttta gtgaaaaatt atgttgtaca agtcggatat cttgtaattc
     3181 cattggtacg gcgaatatca ttaagtatac ttgtcgagaa aagtgaccaa aataacaatt
     3241 atattaaatt aagttatttc ttttcttatt tacttgttgc atattttttg gggggcgcaa
     3301 ttgtttctaa attaatgctt aatatagtat ttgggaataa tattagtgaa tatctccccg
     3361 tatttataat aatgttgatt acatgggctg taagagtcgc tggtgggagt gtattgtcat
     3421 cttattttgt tgtaaaagga aaaccaaatt atgattttta ttggaattta cttcaaggta
     3481 gtattatagc ctgtgtggca atattcacag aatataaaaa cttacaagat ttagctgtac
     3541 atatttttat cgcttattcc ctctacgtta ttattgccgc acctgtattt tgttacttgt
     3601 taaaaagtga gtggagtaaa tcatttttta ctaacttcgt tgttatatcc atttgtggga
     3661 taattgtcat taatagaaat ttgttaatta gcttaacaat aactgaatct atgggtgtta
     3721 tatttattta tgggctgttg ttctgtctgc tctcctattt ttttgttaga aaacacataa
     3781 tataagagat attccatgac tatcgtcaca aataggatgt tgttattttt tatctttttg
     3841 ataataaatg ttcccaatgt tgaagtggaa tatggaggct caattctaaa aatatataac
     3901 gctatttttt ttcttttgtt actattatat ataaaaaaac cgcagtggaa actgcacatg
     3961 ataagttcat atttgttatt tattgtcctt tttttcaccg catatctaat catcaatgga
     4021 agagatgagc atttaattgc gattgcaaat tcttatatac ccaatgagtg gcaagtgtta
     4081 tatgggaaac aagtttttga tgatttctat agaagtacta cagtatcact atttcagtta
     4141 gctaactttc taattttagt tttctatttc aacttttttc acaatgttaa tcggtcgcta
     4201 tttctttttg attcaatgaa atatgtattg gccataagta ttctgactca gatctccata
     4261 tcaataatac agttagttgc atttaataat gatagacctt cgggtagttt tggaaatgct
     4321 caatcacttg gtttttttta tctgttgtgt atctgttatt attctgtgtt tttgccatcg
     4381 tggaaaaaat taatatctat aatcgttcta ttattggcaa ttattattac aggtacccgt
     4441 tcagcattgt tcatagcaat aattattact gctctaaata tgctgaatat atctgtattt
     4501 atgcgaaagc taatattgtt cttattattt attagtgtgg tgattttatg ctctttattt
     4561 tcatcatctg atgtttttcg aggtgttatt ttagaaataa taggcttgtt cacaagccca
     4621 cattcaatgt atgtccgtgg attgatgtgg aattcatttt atcaaacact aactgaaagt
     4681 ccattatttg gtacaaaggg aataactgtc tattttgccg ataatttttt ttggtttttt
     4741 attctttctt atggtatttg tggtgcggta tttattggtg tgttttttct ttcaataata
     4801 agatctgcaa atagtaaatt tgtgtttttc ttaggtgtct gtactatact tcagtcagta
     4861 agttattatg gtttttttat agaaaatatg ggggttgttc taactatatt tttagctatc
     4921 tcattaaacc ataaagttaa aataggctat taaatatgag agttacatta ctatccatca
     4981 attcattaga agaaaataca ggaggaggta tttatctaag atgccttcaa aagttgtact
     5041 taaaaaatgg tgcaaatgtt aatgtcgttt gtaaatcaca caataatcgt tatcgtaaga
     5101 atgttctaac tgatttactc agtagaatta tttttcttag cccatctttt ttagcatgct
     5161 atttatttca agtaatacgt caatgtaaag acagtgacat aatcgcaatt catagttcaa
     5221 gactaggatt ttatgcttgg attctgaaaa aaatatatcc acataaaaaa atatatgtcc
     5281 atacggataa tgttgagttt aatctattaa aagataactt attgcataaa aatatatttc
     5341 gacgcatact ttataatatt gattttttct taatccccat gtcagaaaaa atgtgtgttc
     5401 ggtggggaga taaagttaca ttcattactc gagaagatgc aatagattat aaaagaatgt
     5461 atgcgctcac ttataatgag agacatatat tacctatcta tttggagcca aatgacataa
     5521 caaaaaaaaa tcatagtcaa cgaaataaaa taatctttac tggtagtttt gatttttatc
     5581 ccaatcaaga agtagtatct agaattatta aaattgctga gcaaacacca accttgcaat
     5641 atattgttgc gggaagaaga ttagatattt ttattaaaaa tgcaggtatt caggctcctg
     5701 acaatgtcac atttgagagt gacattgagc ctgagcgatt agaacaactg tatctcgagt
     5761 caagattatt tttatgccct gtaagacatg ggagtggaat gaaaactaaa attgctgagg
     5821 ctttgagcta tggtttgtat gtaatcgcaa atactcgcag tacatgtgga tatgaaactg
     5881 caatagaggc cggcgttata tcatgtgtaa atgatacaac ttttattata gacgcaacca
     5941 gtgagattgt tgattgcttt agccgagaag acaaagatat tatttacaaa tcacttcatg
     6001 tttttaatga aaattataat ttaaataatg gcgtgaaata tttttcagag atattatata
     6061 aatgaagaag attttaattt taacacctcg atttcctttt cctgttatag gtggagatcg
     6121 attgagaatt tataaaatat gtgagaattt gtcaaaaaaa tatgacttaa ctctcctgtc
     6181 actttgtgaa agtgaaaagg agatgaatat gcagattgaa gataacgtat ttaaaaaagt
     6241 ctatagagtg aaattatctc ctttatattc atatttgaat gttttattgt caataccaac
     6301 aaatatacca ttgcaaatag cctactataa atctacaaaa tttcgtaagc taatcgatga
     6361 attattacct gagcatgatg catgtattgc acacctcatt agggtcggtg attatataaa
     6421 agataataag ggaattcgaa tattagaaat gacagatgct atttctctta actataagcg
     6481 agtaaaaata aatacaaata ctaatttgcg atctataatt tatagcattg aacaaaaaag
     6541 attagagcac tatgaaagaa aaatggctag tcattttgat ctagtctctt ttgtttccca
     6601 tatagataaa gatttcctat ataaagataa aaataaaggt aatgttaaag tttactctaa
     6661 tggtgttgac acaactctat taaaattcaa acggcgacat ctaaaacatg acagaccaat
     6721 agagataata tttattggta atatgtattc tttacaaaac atggatgctg ttatttattt
     6781 tgcgcatcaa attttacctt atttaaatcg ggaagggaat tactttaatt taaaagtaat
     6841 tggcaagatc cgagaagtag acaggaggaa attacagcta attaagaatg tgactgtgac
     6901 aggtatagtg aattctgtct cagaagcagc tgaggatgga cacataggaa tttgccctat
     6961 gaggattggc gctggtttgc agaataaaat tttagaatat atggcgttag gattgccatg
     7021 tataacgtca accgtaggtt ttgaagggac tggtgcaaca cgagatagag agatatttgt
     7081 tgctgacacg cttacagaat acaaagagat ttttgaacaa ctattatcta atcatgggct
     7141 atatgagaat gttgctctca atgctagaca gtttgttgag actaaatttt catggaatgc
     7201 ccaacttggt tcaatgattg gtgatattga taacttatta ggtgaataaa atgatactcc
     7261 cagtaattat ggcaggtggt tctggtagtc gtctgtggcc attatctcga actcattatc
     7321 caaaacaatt tcataattta cttggcaaat ctagtttgct aattgatacc attactaggc
     7381 ttgataacat caaacatcag ccccctttag taatctgcaa tgaagaccat cgttttttag
     7441 tagcagaaca gtttagaaag cattcattat caacaagtgg tataatatta gagcctatag
     7501 gtaaaaatac ttgtccagcg attgccttgg ctgcttttca ggcagtgaaa actaacgaag
     7561 acccgtatct tttagtgtta gcagctgatc attcaatagg cgatagagat agctttcaaa
     7621 aggctataca tcaggcatat gaaacggcta aaacgggctc attagttacc tttggaataa
     7681 taccgacgag cccagaaaca ggatatggtt atataaaaaa gggtaaaagc ctcaataaat
     7741 atgcttatta cgtggatggt tttaaagaaa aaccaaatat aatccttgcg aatgaataca
     7801 ttaactcagg gcattatctt tggaatagtg gtatgttcct ttttaaagca tcttcatttc
     7861 taaaagagct taaaaaattt gagccgaaaa tctatcgatt atgtgaagaa tcaattgata
     7921 actctacgca tgatttggac ttcatcagaa ttgattctga tgcattcagt aattgcaaga
     7981 gtcagtctgt agatttcgca ataatggaac aaactaaaaa ttgtgcagtg gttcctatga
     8041 atgctgactg gagcgatgtc ggttcttggt ctgcattatg ggaattatca aaaaaagatg
     8101 atagaaataa ttatttttat ggtgatgtag ttgcaattga ctgtgatagt aactttatcc
     8161 gtacggatga aaagttgact gttactttag gtgttaaaga tcttattgta attaatacaa
     8221 aagatgcact attagtcgct gagcgttctt gcgcgcaaaa agtaaaagat gttgttgcta
     8281 agttaaacgt ggattataga aatgaaatat ctcattacag caatgaattc cgttcttggg
     8341 gagaaataga aaaagttgat agtgataata aatacattgt taatagaata ataattaaac
     8401 ctggtgcaaa aatttcaaag caaattcatt atcatagaag tgagcattgg attgtggtat
     8461 caggtactgc ttctatagta attgatggta aggaatctgt tttgacagaa aatgagtcta
     8521 catttattaa agtaggtcaa gagcactcta ttatgaatcc aggtttggtt ccactaatta
     8581 taattgagat tcaatcaggt agctatgttt ctgaagatga tattataaga gtcaaggaga
     8641 tttacaatga tgagtgctaa tatcgtttgt caaaatatta taaatgtagg aaaaataact
     8701 tttggcacaa gtggtgctcg cggtttagtg atcgattttt cccatgatgt ttgtgctgcg
     8761 ttcactcatg cgtttctttc tgttattgat gacaaataca attttaataa agttgcctta
     8821 gcaattgata accgcccaag cagttacgaa attgctcagg catgcgcctt cgctatcaaa
     8881 caacacgggt acactgtcga atatcatggt gtaattccga ctcctgcatt agctcattat
     8941 tcgatgcaga aaaacattcc ctgcatcatg gtcacaggga gtcatatccc ttttgatcgt
     9001 aatggcttaa aattctacag acctgatggt gaaatcacta aagaggatga gctcgcaatt
     9061 ataaatagtg agtatacatt ttcgcctgta ggtgtattac ctcatcttga aacaagcact
     9121 caaggtgcag actactacct tgagcgttat gtttctcttt ttaatcccga aattttaaag
     9181 gggaaaagaa taggggtata tgaacactct agtgcaggac gcgatttata tgctcttctt
     9241 tttaatcaat tgggtgcaga ggtcatttcc ttgggtagaa gtgatgagtt cgttccgatt
     9301 gatacggaag cagtaagtga tgaagatcgt atacttgcaa gagagtggtc taaaaaatat
     9361 aatcttgatg ctattttctc aacagatggc gatggtgatc gtcctttagt tgccgatgaa
     9421 aatggtgaat ggctaagagg cgatattctg gggttactta ccgctattga acttaatatc
     9481 aaggcgttgg ctattccagt gagttgtaat acagcaattg aacagtctca taaatttgca
     9541 agtatacaac gaacgaaaat aggctctcct tatgtaattg cagcgtttgc agatcttgct
     9601 aagcaattta attcagttgc tggttttgaa gctaatggcg gttttctcct tgcctccgat
     9661 ttacaaatta atggcaagga attaaaatcg ttacctacac gagatgctgt gttaccagca
     9721 ttaatgctct taatagcttc tcgcaatagt actatttctc aactgattaa taatcttcct
     9781 cagcgattca cttggtcaga tagacttaaa aacttccctt cagattcaag tcaacaaatt
     9841 ataaaaaatg ccatatcgtc acccaataat ttctttaata gtttaggtta tgaatcatta
     9901 tcctgctccg ctattgatga aacggatggt gcaagattta ctttaaataa tggtgatatt
     9961 atacatctcc gtccttccgg taatgctcca gaactccgtt gttatgctga ggccagtgat
    10021 gaaaatcagg ctaggcaata tgttacgaat gtgctgggaa atattacctc tttgatttct
    10081 tgatgttata ggtttatcta cgcttatatg tgtgcgtagg tttgattaca cgtagatgcc
    10141 ggtatacaga attgaagaac ggtatttatt acattaatga aattcagcac tacacacatt
    10201 cgtgcaactt gagataacat ctcaatcata ttcaagtcgc gcatacatcg cggtgaacac
    10261 cccctgacag gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta tggcagtgat
    10321 ggggcgcaac cttgcgctca acatcgaaag ccgtggttat accgtatcta ttttcaaccg
    10381 ttcccgtgag aaaacggaag aagtgattgc cgaaaatccg ggcaagaaac tggttcctta
    10441 ctatacggtg aaagagtttg ttgaatctct ggaaacgcct cgtcgcatcc tgttaatggt
    10501 gaaagcaggt gcaggcacgg atgctgctat tgattccctc aagccatacc tcgataaagg
    10561 tgacatcatc attgatggtg gtaatacctt cttccaggac accattcgtc gtaaccgtga
    10621 gctttctgcc gaaggcttta actttatcgg taccggtgtc tccggtggtg aagaaggtgc
    10681 gctgaaaggt ccttccatta tgcctggtgg ccagaaagaa gcctatgaac tggttgcacc
    10741 gatcctgacc aaaatcgccg cagtggctga agacggcgaa ccgtgcgtta cctatattgg
    10801 tgccgatggc gcaggtcact atgtgaagat ggttcacaac ggtatcgaat acggtgatat
    10861 gcaactgatt gctgaagcct attctctgct taaaggtggt ctgaacctct ccaacgaaga
    10921 actggcgcag acctttaccg agtggaataa cggtgaactg agcagctacc tgatcgacat
    10981 caccaaagac atcttcacca aaaaagatga agacggtaac tacctggttg atgtgattct
    11041 ggatgaagca gcaacaaagt accggttaat ggaccagcca gagcgcgctg atctcggcga
    11101 accgctgtcg ctgattaccg agtctgtgtt tgcacgttat atctcttctc tgaaagatca
    11161 gcgtgttgcc gcgtctaaag ttctctctgg cccgcaagcg cagccagctg gcgacaaggc
    11221 tgagtttatc gagaaagttc gtcgtgcgct gtatctgggc aaaatcgttt cttacgctca
    11281 gggcttctct cagctgcgtg cggcgtctga agagtacaac tgggatctga actacggcga
    11341 aatcgcgaag attttccgtg ctggctgcat catccgtgcg cagttcctgc agaaaatcac
    11401 cgatgcctat gccgaaaatc cgcagatcgc taacctgctg ctggctccgt acttcaagca
    11461 aattgccgat gactaccagc aggcgctgcg tgatgtcgtc gcttatgcag tacagaacgg
    11521 tatcccggtt ccgaccttcg ccgctgcggt tgcctattat gacagctacc gcgccgctgt
    11581 tctgcctgcg aacctgatcc aggcccagcg tgacta
//
