LOCUS       DQ875941               10432 bp    DNA     linear   BCT 17-SEP-2006
DEFINITION  Shigella boydii serotype 17 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ875941
VERSION     DQ875941.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Senchenkova,S.N., Feng,L., Wang,Q., Perepelov,A.V., Qin,D.,
            Shevelev,S.D., Ren,Y., Shashkov,A.S., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic characterization of Shigella boydii type 17
            O antigen and confirmation of two new genes involved in the
            synthesis of glucolactilic acid
  JOURNAL   Biochem. Biophys. Res. Commun. 349 (1), 289-295 (2006)
   PUBMED   16930547
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Senchenkova,S.N., Feng,L., Wang,Q., Perepelov,A.V., Qin,D.,
            Shevelev,S.D., Ren,Y., Shashkov,A.S., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2006) Nankai University, TEDA School of
            Biological Sciences and Biotechnology, 23 HongDa Street, TEDA,
            Tianjin 300457, China
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="17"
                     /db_xref="taxon:621"
     misc_feature    1..10432
                     /note="O antigen gene cluster"
     gene            926..2191
                     /gene="wzx"
     CDS             926..2191
                     /gene="wzx"
                     /note="O unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ABI55339.1"
                     /translation="MKKKKAASHLIILTVGTLLTAFLNFATQIILARHMIPSDFGSFS
                     SAFYLVMTLAPLCGFGIPQFWLKVFGSEGWAGTRFIVPSLIFTAITTSLIIMLLSGWF
                     YYTEYNKHEFLYLLSFVVLSQVIIELLGAVYQLEENYIAVSLIQLSVISLRFISLIFL
                     FLIFKHLTTDSVSLIYALDSLIIIIFTYGVFRRIIKKEIKLKGHIKNDNIQSLTINIK
                     DMIKESAVFGIAGVFYMIYFQVNIILVKYFISPIDAGYYSVAFLFISAAILLPTVIYQ
                     KYLLPKLHRWAYQDLKKLKNVYKYGNISMFLFGVVVSILIWFLCPMIVYSLFGEKYMS
                     SIKLIRFLSLSIPIIYLASNSGAILVTKNNMQAKVKIMFVASFISLLVFFVTFRNMGV
                     YAGVLATILSNLFICIAYNYKSIVFFRGE"
     gene            2191..3555
                     /gene="wfaW"
     CDS             2191..3555
                     /gene="wfaW"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaW"
                     /protein_id="ABI55340.1"
                     /translation="MSIQKVIKKNYCIGCGACSYNNSDVTIEWNELGLLIARGDIEAL
                     DDDICPFASSRDETFIAKLLYGNIPGLEFNEKIGYYHGLFAGYANEGTYRDNGSSGGL
                     TTWLLVELLKNNMIDGVIHVGKSKSSEQIYSYTISESIKEVQDNTRSQYYPTKFDCAL
                     ENIDINKKYAIVGIPCYIKAVRLLCDKNDQLKKSIKYFIGIFCGHLKSKAFAELLSWQ
                     QGIDPEYLKNINFRVKNSTGTASRYNISVTSFTGVTRMAQTGHLYGTDWGLGLFKPQG
                     CEWCDDIAAELADVVFGDAWLPNYVSDPKGTNIVISRNKEISSLMNKALREQRLHLDN
                     ILVDDIVKAQAGNYRHRREGLKVRIQDADLKNIWHPKKREFLLPKKISDERKQIYRVR
                     NAISNLSHVYFLKAKNKKNLMLFFVNMGAQELKYRFYTKTLLKSTLKFMYYIMKYLKA
                     KGAK"
     gene            3552..4655
                     /gene="wfaX"
     CDS             3552..4655
                     /gene="wfaX"
                     /note="pyruvyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaX"
                     /protein_id="ABI55341.1"
                     /translation="MSKKVGIVTLPLVSNYGGILQCLALNFVLRKLGYKVTLIDIKPR
                     PPVIKGIILNILSRCPFQNIKGIREQYVKEKKNENTISKYINNRTGKITDSSGLEKAF
                     NNLDLDAVIVGSDQVWRYEYINNDMYAAYFLNFVKEKKCKKISYAASFGVDNWNHENK
                     RSVTQKLLSQFDAISVREISGVQICNNEFAVKNVEHVLDPTMLDLTFYDELLDGVNEK
                     KEFDKKYLLKYVLDEKDIADNISLLINKKYGLSNEVNILGKGKNYSIEEWVLAFKKAT
                     YIVTDSFHGMVFSILFNKNFIVIGNKSRGLSRFVSLLGKLDLLDRLFYPEEIINNKRL
                     IEILHKDIDYCNVNELIKNWRGNSIRFLKTNLN"
     gene            4681..5892
                     /gene="wzy"
     CDS             4681..5892
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ABI55342.1"
                     /translation="MINNGWFFNMYVNYISYKLIAILGGAAFLLIPFSPILSFSISIF
                     TLLTMSNYLFGYMRQSLIILAMISIVIIIASREYTDEIEHDLSHYYIVYKALASGQLN
                     ELFGFAAGVEIGWPLLYLMVSKFNGNLSPITLAIINTSVCLFLLYVWINKHAFHNEYL
                     NNSGVIAALIILFASVQTFGYLQRQAISTVILLFSISNTGKKSLVYLLLATLFHLTSL
                     PLGILYLTLRKWHSRIKLHHMLMIFGILIFIKLNLFVILNYLSVVGTGIPGVNKLNYY
                     LLYASEFSFTSKRFAILIFPLALAMILFWNKIPNDNWKLISIFSCLSYIVFLGIPLGP
                     ERLNFLLLFLYGYFVYLYLYKVLPNVTKYFILVYLFMFALEKMNFFGNYADPFWSRYP
                     AFSFEPFYYLY"
     gene            5909..6850
                     /gene="wfaY"
     CDS             5909..6850
                     /gene="wfaY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI55343.1"
                     /translation="MMFLTVVIPLYNKSQSIESTIKSVLNQTHTDFELIVVNDGSTDG
                     SENVIKQFTDERIKLLNITNSGVSVARNTGIRSAKSSYVCLLDADDYWLPNHLEKINN
                     LVERYPDCSMYSSCFYEILEDGSKFQAKNRYPSDYIGILDDFFLEYSRSRSLVHSSCV
                     CLNKKMFAECGGFPVGVKLGEDIYLWLKMAMLGQVAYSGSPQVIIRRNAENRTATRVD
                     NDILYHIKYFHENPSEKNKLPTKMHHSLDVFLKKNIFVNALFTLSIKDEITFNKYLTA
                     MREISCMYYIILGSLSFVLTPYMLKLLRKIRNLLQNS"
     gene            6890..7906
                     /gene="gne"
     CDS             6890..7906
                     /gene="gne"
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ABI55344.1"
                     /translation="MSILVTGGCGYIGIHTVYTLISQGYSVVVLDNLTNSTMEPLRRV
                     EFMTNRSIPFYEGDVGSERLLEEIFIEHHITSIIHFAGLKSVSESILNPVEYYLNNVA
                     QTLSLLNAMIKNNIKDLIFSSSATVYGKPENCPITESENTGGTTNPYGTSKYIMELIL
                     SDVCKAYHDLNITVLRYFNPIGAHESGNIGEHPNGVPNNLFPYLTQVAIGAHPYLNVL
                     GSDYPTPDGTGVRDYIHVMDLAEAHVKALLRNTSCPGLKTYNLGTGKGYSVFDIIREF
                     EVVTGVKIPYKVMPRRNGDVAECWSDSSKANKELQWTAKRDLKDMIRDAWHWQSKNPH
                     GYDS"
     gene            7929..8987
                     /gene="wfaZ"
     CDS             7929..8987
                     /gene="wfaZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI55345.1"
                     /translation="MKTDNRKRLVFIITKSEVGGAQKWVSEQKLLLEDKYDTFLITSC
                     TGWLTDNFSPDKVFFVPALTNIKKISNLFSIAKILRMLKADIVVSNSANAGLYARLAK
                     IIWKHRSIYVSHGWSCIYNGGRAKKILCFIERFLSFFSDAILCVSENDKDNALNIIGI
                     KESKLKLIKNATFPTNKEKKFWHIMPKVLRLVFVGRMTHPKRPDLLAETLSRKKDVEL
                     FLVGGGEYLERLKNIYKNYDNIHFVGEIKDFNNYDDYDAFILVSESEGLPMSAIEAGV
                     TGLPLLLSDVGGCHELIGEYEGKYNGVLFNNNINDISRAIDEVRNNYEQYCKVANKIS
                     CQFNLNSFKEDYIVLYEG"
BASE COUNT     3366 a   1592 c   2059 g   3415 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 cagctgctgg cggaagtgca gtcaatctgt ccaccgggag tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttgtgtg cgcgacctgc cattggtgac
      241 aacccatttg ccgtggtact gccagacgta gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccacaa
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaactagaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgctattgcc
      601 gaactggcaa aaaaacagtc cgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcgttt gtaaagtatg gattgcgcaa tctgaaagaa
      721 ggggcgaagt tcagagaaag tatagtgaaa atgatagagt agataatcaa aacaatatta
      781 gtatcgcaat attcaagcga tattaatatt gttcattttc aaattggctg actaagaata
      841 aaacagacca atcaacgccg aaaattaata aggaatatag tgcactggta gcttgtgagc
      901 caggggcggt agcgtggagt gttaaatgaa aaagaagaag gctgctagtc atttaattat
      961 acttacagtt ggaacgttgt taacagcatt tttaaatttt gctacacaaa ttatacttgc
     1021 acgacatatg attccaagtg attttggctc attttcatcg gcattttatc tggtaatgac
     1081 attggctcca ttatgtggat ttgggattcc acaattttgg ctaaaggtat ttggaagtga
     1141 aggctgggct ggaacaagat ttatagtacc gagtttgata tttacagcta taaccacatc
     1201 acttattata atgttgcttt cggggtggtt ttattacaca gaatataata agcatgaatt
     1261 tttatattta ttatcatttg ttgtgctctc tcaggttata attgagttgt taggtgctgt
     1321 ataccaattg gaggaaaatt atattgcggt ttcgttgatt caattatcag taatcagctt
     1381 gagatttata tcactaattt ttcttttctt gatttttaaa catttaacaa ctgattcggt
     1441 gtctttaatt tatgcgcttg attctcttat cataattata tttacgtatg gtgtatttag
     1501 acgcattatt aagaaagaaa taaaattaaa aggacacatt aaaaacgata atatacaatc
     1561 attaactatc aatataaagg atatgataaa agaatcggca gtctttggta ttgctggagt
     1621 attttacatg atatatttcc aggtaaatat aatacttgtt aaatatttta tctccccaat
     1681 agatgctgga tattacagtg tggcttttct atttatttct gctgcaatat tgttgccgac
     1741 agtaatatat caaaaatatt tactcccgaa acttcatagg tgggcatacc aagacttgaa
     1801 aaaattaaaa aatgtttata aatatgggaa tatatctatg ttcttattcg gcgtagttgt
     1861 ttccatatta atttggtttc tttgcccaat gattgtttat tctttgtttg gggaaaagta
     1921 tatgagttct ataaagttga ttcgattttt atcattaagt attcctatta tttatcttgc
     1981 aagtaattca ggggccattt tagtcactaa aaataatatg caagcgaaag ttaaaattat
     2041 gtttgtagct agctttatta gtctcttggt tttttttgta acttttagaa atatgggggt
     2101 ctatgctggt gtgttagcta ctattttgag taatttattt atttgcatcg catataatta
     2161 taaaagtatt gtcttttttc ggggggaata atgagtattc aaaaggtaat aaaaaaaaac
     2221 tattgtattg gatgcggagc atgttcctat aataatagtg atgtaactat cgaatggaat
     2281 gagctaggtt tacttatagc aagaggggat attgaagctt tagacgacga tatctgtcca
     2341 tttgctagtt cgagagatga aacctttata gcaaagttat tatatggcaa tataccaggt
     2401 ttggaattta atgaaaaaat tggatattat cacggattat ttgctggata tgctaatgaa
     2461 gggacttata gagataatgg gagttcggga ggtcttacaa catggcttct tgttgaactt
     2521 ttaaaaaata acatgatcga tggtgttatc catgttggaa agtctaaaag tagcgaacag
     2581 atttatagtt atactatttc tgaatcaata aaagaagtac aagataatac acgttcacaa
     2641 tattacccaa caaagtttga ttgtgcgttg gaaaatatag atataaataa aaaatatgcg
     2701 atagtaggta taccttgtta tatcaaagct gttcggttat tgtgtgataa aaatgatcaa
     2761 ctgaaaaaat cgattaaata ttttattggt attttttgcg gacaccttaa gtctaaggca
     2821 tttgcagaac tactgtcttg gcaacaagga atagatcctg aatatttgaa aaatataaac
     2881 ttccgagtca aaaattctac tggaacagca agtcgatata atataagtgt tacatcattt
     2941 acaggtgtta ctcgaatggc tcaaacaggg catttatatg gtactgactg ggggctaggt
     3001 ttatttaaac cacaagggtg tgagtggtgt gatgatattg cagcagaatt agccgatgtt
     3061 gttttcggag atgcttggtt acctaattat gtttcagatc caaaaggcac aaatattgta
     3121 atatctcgga ataaagagat aagtagttta atgaataaag ccttaagaga gcaaagatta
     3181 catttagata atatacttgt cgatgatatt gttaaagcac aagccggtaa ttataggcat
     3241 cgacgtgaag gattaaaagt tagaatacaa gatgcggacc tgaaaaatat ttggcaccct
     3301 aagaaaaggg aatttttatt acctaaaaaa atatctgatg aaagaaaaca aatatataga
     3361 gttagaaatg caatctcaaa tctctcacat gtttattttt taaaggcaaa aaataaaaaa
     3421 aacttaatgt tattctttgt taatatgggg gcgcaagaat taaaatatcg gttctacaca
     3481 aaaacattat taaaaagtac tcttaaattt atgtattata taatgaagta tttgaaagct
     3541 aaaggtgcaa aatgagtaag aaggttggta ttgtcacttt accattagta tctaactatg
     3601 gtggtatttt acaatgttta gcattgaact ttgtactaag aaaactagga tataaagtta
     3661 ctttaattga tatcaagcct cggccacctg ttataaaagg tattatctta aatatacttt
     3721 caagatgccc ttttcaaaat ataaaaggta ttcgggagca atatgttaaa gaaaagaaaa
     3781 atgaaaacac cattagcaaa tatataaata acaggacagg taaaattaca gattcttctg
     3841 gtttagaaaa agcatttaat aatcttgatt tagatgcagt tattgtgggg agtgaccaag
     3901 tttggcgata tgagtatata aataatgaca tgtatgcagc ttattttttg aattttgtta
     3961 aagaaaaaaa atgcaaaaaa atatcttatg cagcttcatt tggtgttgat aattggaatc
     4021 atgaaaataa aaggagtgta acacaaaagt tactcagtca attcgatgca atttcagtgc
     4081 gtgaaatttc aggtgtacaa atttgtaata acgagtttgc agtaaaaaat gtagagcatg
     4141 ttttagatcc aacaatgctt gatctaactt tttatgacga acttcttgat ggggttaatg
     4201 aaaaaaaaga gtttgataaa aagtatttgc tcaaatatgt tttagacgaa aaagatatag
     4261 cagataacat tagtcttcta ataaacaaaa aatatggttt atcgaatgaa gtaaatattt
     4321 taggcaaagg caagaactat tcaattgaag aatgggtttt agcatttaag aaagcaacat
     4381 atattgtaac agactccttt catggcatgg tgttttcaat tttatttaat aaaaatttta
     4441 ttgtaatagg gaataaatct cgtggcttga gtcgttttgt atcgttactg ggaaaattag
     4501 acttgttgga caggcttttt tatccggaag aaataattaa taataaaaga cttatagaga
     4561 ttttgcataa ggatattgat tactgtaatg taaatgaact cattaaaaat tggaggggaa
     4621 actctatacg tttcttaaaa acgaacctaa actagataag ttataatata ttcattgata
     4681 atgattaata atgggtggtt ttttaacatg tatgtcaatt atatatctta taaattaata
     4741 gctatattag gaggagcagc atttttatta ataccgttct cgccaatact tagtttttct
     4801 atttcaatat ttactcttct tactatgtct aattatctct ttggatatat gaggcaaagt
     4861 cttatcatat tagctatgat ttctattgtt attataatag catctagaga atatacagat
     4921 gaaatagaac atgatttaag tcactattat attgtatata aagcattggc ttctggacag
     4981 ttgaatgagc tttttggttt tgctgcaggg gtagaaattg gttggccatt attgtacttg
     5041 atggtatcta aatttaatgg taatttatct cctataacct tagctataat taatacatct
     5101 gtttgcttat ttctattata tgtttggatc aataaacatg catttcataa tgaatatctt
     5161 aataactcag gagtgatagc agcattaatt atactatttg cttctgtgca aacttttgga
     5221 tacttacagc gacaggctat atctacagtc atattacttt tttcaatttc aaatactggg
     5281 aaaaaaagtt tagtatactt attgctagca accttattcc acctaacgtc tctaccatta
     5341 ggaatactat atttaacttt aagaaaatgg catagtagaa taaaactaca tcatatgtta
     5401 atgatttttg gtattcttat atttattaag ttaaacttat ttgttatatt aaattatctt
     5461 tcagttgttg gtactggtat tcctggggtt aataagctta actattatct attgtatgct
     5521 tcagaattta gttttacatc gaaacgattt gcaattttga ttttcccttt ggctttagca
     5581 atgatattat tttggaataa gattccaaat gataattgga aactaatttc aatattcagc
     5641 tgcctttcgt acattgtctt tttgggtata cctctcggcc cagaaaggtt gaacttttta
     5701 ttactatttc tttatggtta ctttgtgtat ctgtacttgt ataaagtatt gccaaatgta
     5761 actaaatatt ttattttagt atatttattt atgtttgcat tagaaaagat gaactttttt
     5821 ggaaactatg ctgatccatt ttggtctaga tatccggcat tttcatttga gccattttac
     5881 tatctttact agctaatatt aagggattat gatgttttta acagtagtta ttccgcttta
     5941 taataaaagt caatcaattg agtccacgat taaatctgtt ctaaatcaaa cgcatactga
     6001 tttcgaatta attgtagtaa atgatggttc aacggacgga agtgaaaatg taataaaaca
     6061 gtttactgat gaacgtataa aacttttgaa tattacaaac tctggagtat cagttgctag
     6121 aaataccggt ataagatcag cgaaatcaag ctatgtgtgt ttattagatg cagatgacta
     6181 ttggttacct aatcaccttg agaaaataaa taatttagtt gaaagatatc ctgattgttc
     6241 catgtattca tcgtgttttt atgagatttt agaagatggt tctaaatttc aagctaaaaa
     6301 tagatatcct agtgattaca tcggtatttt ggatgatttt tttcttgagt actctcgttc
     6361 tagatccttg gttcactcat cttgtgtatg tttgaataaa aaaatgtttg ctgaatgtgg
     6421 tggttttcct gtaggagtta aacttggtga agatatttat ttatggttaa aaatggcaat
     6481 gctaggacag gttgcatatt cagggtctcc gcaggttatc attaggcgga atgcagaaaa
     6541 tagaactgca acgagagttg ataatgatat tttatatcat ataaaatatt ttcatgaaaa
     6601 tccaagtgaa aaaaataaac ttccaactaa aatgcatcat tcacttgatg tttttttgaa
     6661 gaaaaatatc tttgttaacg cactctttac attatccatt aaagatgaaa ttacattcaa
     6721 taaatactta acggcaatgc gcgaaattag ttgtatgtat tatattattc ttggctcgtt
     6781 gagctttgtt cttactccat atatgttaaa actattgaga aaaattagga atttacttca
     6841 gaattcatag aagctaaaac tggtgaatta attaataatt ggtgaagtca tgtctatttt
     6901 agttactggt ggttgtgggt atattggaat tcatacggtt tatactctga tttctcaagg
     6961 atattcagtt gttgtcttgg ataacttaac gaattctaca atggaacctc tgcggagggt
     7021 tgagtttatg acaaatagaa gtatcccttt ttacgaggga gatgttggta gtgaaaggtt
     7081 attagaagaa atatttattg aacatcacat aacctccatc attcattttg ctggactgaa
     7141 atcagtaagt gaatctatac ttaatcctgt tgaatattat ttaaataatg tagcacaaac
     7201 attgtcattg ctaaatgcaa tgataaaaaa taatattaaa gatttgattt ttagttcatc
     7261 tgcaactgtc tatggtaaac ctgaaaactg ccctattact gaatcggaaa atactggtgg
     7321 tacaacgaac ccatatggta cttcaaaata tataatggaa ttgatccttt ccgatgtatg
     7381 caaggcatat catgatctca atataactgt tttacgatac tttaatccaa tcggtgcaca
     7441 tgagtcgggg aatattggtg aacacccaaa tggggttcct aataacttat ttccttattt
     7501 gactcaagtt gcaattggtg ctcatccata cttaaatgta ctgggaagcg attatccaac
     7561 tcctgacggt acaggtgttc gtgattatat ccatgtaatg gatctggcag aagctcacgt
     7621 caaagcttta ttgagaaata catcttgtcc tggtttaaaa acgtataatt taggaacggg
     7681 taaaggatat tctgtatttg atattattcg agaatttgaa gttgttacgg gagttaaaat
     7741 accatataaa gttatgccgc gaagaaatgg tgatgttgct gaatgttggt ccgatagttc
     7801 gaaagctaat aaagaattgc aatggactgc caaaagagac ttaaaagata tgattcggga
     7861 tgcttggcat tggcagtcaa aaaatcctca tggttatgat agttaaataa gagattagtg
     7921 gcaattatat gaaaacggat aatagaaaaa gactagtttt tataatcact aaatctgagg
     7981 ttggtggagc tcaaaaatgg gtaagtgagc aaaaattact tcttgaagat aaatatgaca
     8041 catttttgat tacgtcatgt actggatggc tcacagataa tttttcacct gataaagttt
     8101 tttttgttcc agcactgact aatataaaaa aaatatcaaa ccttttctct attgcaaaga
     8161 tactaaggat gcttaaggct gatattgttg taagcaattc agcaaatgcg ggtttatatg
     8221 ctcgtttagc taagataata tggaagcatc gatcgatata tgtctcgcat ggttggtcat
     8281 gcatatataa tggtggacga gcaaaaaaaa tactttgttt tatcgaaaga tttttatcct
     8341 ttttctcaga tgcaatattg tgtgtatcag aaaatgacaa ggataatgcc ttaaatatta
     8401 ttggtataaa agaatcaaaa ctgaaactta taaaaaatgc aaccttccct acaaataaag
     8461 aaaaaaaatt ttggcatatt atgccaaaag tgttaaggct agtttttgta ggccgaatga
     8521 ctcacccgaa acgccctgat ttattagctg agactctgag tagaaaaaaa gatgttgagc
     8581 tctttcttgt aggtggtggg gagtatcttg aacgtcttaa aaatatttat aaaaattatg
     8641 ataatattca ttttgtcggt gagattaaag atttcaataa ttatgatgat tacgatgcat
     8701 ttattttggt ttctgaaagc gaaggtttac caatgtctgc gatagaagca ggtgtaacgg
     8761 gtttgccact tttgttaagc gatgttggtg gatgtcatga attaattggc gagtatgaag
     8821 gaaaatataa cggagtttta tttaacaata atatcaatga tatatctaga gccattgatg
     8881 aggtgaggaa taattacgaa caatattgta aggtcgccaa taaaatttca tgtcaattta
     8941 acttaaatag ttttaaagaa gattatattg tattgtatga aggttaggct acttgccatt
     9001 tctattatca cttttttgaa actatataat tgttgttaga tttaaatatt ttcaagccgc
     9061 tacaggcggt aaccccctga caggagtaaa caatgtcaaa gcaacagatc ggcgtagtcg
     9121 gtatggcagt gatgggacgc aacctcgcgc tcaacatcga aagccgtggt tataccgtct
     9181 ctattttcaa ccgttcccgt gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga
     9241 aactggttcc ttactatacg gtgaaagagt tcgttgaatc tctggaaacg cctcgtcgca
     9301 tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattcc ctcaaaccat
     9361 atctcgataa aggcgacatc atcattgatg gtggtaacac cttcttccag gacaccattc
     9421 gtcgtaaccg tgagctttct gccgaaggct ttaacttcat tggtaccggt gtctccggtg
     9481 gtgaagaagg cgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg
     9541 aactggttgc gccgatcctg accaaaatcg ccgcagtggc tgaagacggc gagccatgcg
     9601 ttacctatat tggtgccgat ggtgcaggtc actatgtgaa gatggttcac aacggtattg
     9661 aatacggcga tatgcagctg attgctgaag cctattctct gcttaaaggt ggcctgaatc
     9721 tcaccaacga agaactggcg cagaccttta ccgagtggaa taacggtgaa cttagcagct
     9781 acctgatcga catcaccaaa gatatcttca ccaaaaaaga tgaagagggt aactacctgg
     9841 ttgatgtgat tctggatgaa gcagcaaaca aaggtacggg caaatggacc agccagagcg
     9901 cgctggatct cggtgaacca ctgtcgctta ttaccgagtc tgtgtttgca cgttatatct
     9961 cgtctctgaa agatcaacgc gttgccgcgt ctaaagttct ctctggcccg caaacgcagc
    10021 cagctggcga caaggctgag ttcatcgaaa aagttcgtcg tgcgctgtat ctgggcaaaa
    10081 tcgtttctta cgcccagggc tcctctcagc tacgcgctgc gtctgaagag tgcaactggg
    10141 atctgaacta cggtgaaatc gcgaagattt ttcgtgctgg ctgcatcatc cgtgcgcagt
    10201 tcctgcagaa aatcaccgat gcatacgccg aaaatccgca gatcgctaac ctgctgctgg
    10261 ctccgtactt caagcaaatt gccgatgact accagcaggc gctgcgcgat gtcgtcgctt
    10321 atgcagtaca gaacggtatc ccggttccga ccttcgccgc tgcggttgcc tattatgaca
    10381 gctaccgtgc cgctgttctg cctgcgaact tgattcaggc acagcgcgac ta
//
