LOCUS       AY948196               12110 bp    DNA     linear   BCT 20-AUG-2005
DEFINITION  Shigella boydii serogroup 18 O antigen gene cluster, complete
            sequence.
ACCESSION   AY948196
VERSION     AY948196.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Feng,L., Senchenkova,S.N., Wang,W., Shashkov,A.S., Liu,B.,
            Shevelev,S.D., Liu,D., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic characterization of the Shigella boydii type
            18 O antigen
  JOURNAL   Gene 355C, 79-86 (2005)
   PUBMED   16055280
REFERENCE   2  (bases 1 to 12110)
  AUTHORS   Feng,L., Senchenkova,S.N., Wang,W., Shashkov,A.S., Liu,B.,
            Shevelev,S.D., Liu,D., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="serogroup: 18"
     misc_feature    1..12110
                     /note="O antigen gene cluster"
     gene            1150..2235
                     /gene="rmlB"
     CDS             1150..2235
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="AAY23333.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2235..3134
                     /gene="rmlD"
     CDS             2235..3134
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="AAY23334.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            3192..4070
                     /gene="rmlA"
     CDS             3192..4070
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="AAY23335.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGGQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCLEEIAYRKGFIDAEQVKVLAQPL
                     KKNAYGQYLLKMIEGY"
     gene            4075..4632
                     /gene="rmlC"
     CDS             4075..4632
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="AAY23336.1"
                     /translation="MKVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSNKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSSENMRQLWIPEGFAHGFLVLSDTAEFLYKTTKYYHPESDSGIIWNDKNIGICWPLR
                     EKPIISEKDSNLLPFAHLTRKQINL"
     gene            5978..7195
                     /gene="wzx"
     CDS             5978..7195
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAY23337.1"
                     /translation="MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAG
                     IQYVNIIVDFGFNFTATKLISIYKNNKNEKNKIFTATIIIKFILFCLCLSVLLLLSLV
                     LEQEKYIQYIIIGSIAVIGNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKS
                     SDDVGLAIASYSLGLLFTGLLSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNIT
                     VSFYTTLNTILVGAIAGPATAGYFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYK
                     LNDVLKTFGYKMIGFGFFLSLSIAIIGVPLSEWYFGNNNAISSKILLILSPLPFVVSI
                     GIVFGQWWLITTNRTRIIRKVYFNLSMLHVFIAYFLIKHIPNYGVAISIVFTETIISL
                     LLTIFAIKNSVNH"
     gene            7313..8296
                     /gene="wbwX"
     CDS             7313..8296
                     /gene="wbwX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAY23338.1"
                     /translation="MPLFSGHNQPRIPLNNNYYNLLDKNTLIWQAGLANQYKIGAMCF
                     YHYWFLGKQLLEKPAEILLENKEINMPFLFSWANEPWTRAWDGSHRNVLMPQSYGTEA
                     DWEKHFNYLKPFFEDERYLKHDGKPIFIIYRTANFALCNEWISCWRELSKNTSFKDIH
                     FVSSYTSFENDPRKLDFDAHLYFEPMCTVGHNLGYFNKKIKKINARLKRVINPKVWTT
                     YVENTLQYDYLWEKILKKKINKTQYPGAFVDWDNSARKKSRALVIHGGSPKKFGLYLD
                     KLYKRSIENNCPFLFINAWNEWAEGTYLEPDEKNKYSYLEELKKVIEKYEV"
     gene            8380..9489
                     /gene="wzy"
     CDS             8380..9489
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAY23339.1"
                     /translation="MYIFILLALWVCQTSINYKINYGFIYVLLFYSFFIISLFFFPSR
                     VKKEPKAYLNGGANKIKKIVKILSYINLIILAYLLYQFYNILQSGIAISVYRSSLFGD
                     SSIKDYYGNAAAYMFFYCTIVSLLTISFGIYWKIYTAENSILLIGLLFLIMKEIVLVS
                     RFYLFPTFLSLMLILYCFDKKITRKRISIYMFLLLMPIIFAFLLKSNGSLDTVFYNAR
                     NYFVVGFSLFTNAIDSELTDQLHYMGSPFAFLGVFSTFFYNKNLFFEKIQEFVSLGDA
                     GYFNAFYTSFLLPYLYFGFIGTIICSIFFGLLISYNLYRLTITQSFFNFNTLFASLPI
                     FFSQQFTPVQLTFFWDYFYLSIVAQFVRRLCLRKI"
     gene            9500..10396
                     /gene="wbwY"
     CDS             9500..10396
                     /gene="wbwY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAY23340.1"
                     /translation="MKNATILVLYNPNIKYVINNINIISELTDLLILIDNTPEHEKRE
                     INKKILLQSISGFDEVYYIGMNNEGLSKAYNEGVKKLNSCCHKIGGVLFLDQDSDVSH
                     ATLKQLWDTYNKLEDICDLGVLGAYPIRVDGIGYRYNVKKENTNILPTNVIEVKRVIS
                     SFSIVPLRVLNELNGFYDDFFIDHIDNDFSRRCIKKNKIVAMDLNAKFIQRIGEGSIT
                     FLGKHITPISAPFRHYYQARNILLSAKRCGDGIIYALPYLVKRFIMINLQGVYGHNLI
                     TRYKFYLLGILHGINNRSGKLK"
     gene            10393..11502
                     /gene="wbwZ"
     CDS             10393..11502
                     /gene="wbwZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAY23341.1"
                     /translation="MKICYVITKADEIGGAQIHVRDLSIQLAKDNNEVFVIVGENGAL
                     VDELVNNNISVYVLPTLVRQINPIKDFQSILKLPKIIKKNKPDGWSLHSSKAGIVGRL
                     AAIGTDIPVIFTAHGWAFANGVGKRQQKLYCAIEKIMSPFAKKIITVSQQDKDLAIKY
                     RVVSAEKQVVIHNGMPEIEMVCDNKKIESYPTIKLISVARFSEQKDHKTLFLALKELK
                     DYDWSLSLVGKGPLIDHYKKYAIELGIFERIKFLGERSDVADLLSESNVFLLISNWEG
                     FPRSILEAMRAGLPVIASDVGGTSESVLNNKTGFLISRNDVHTLKEKIEILIKNEALR
                     NEMGRYAREIYLANFTFDSMYNKTVALYKSLVNIS"
BASE COUNT     3823 a   1968 c   2484 g   3835 t
ORIGIN
        1 atatactata gtattgtggc tgcagggatc aaagaaatcc tcctggtaac tcacgcgtcc
       61 aagaacgcgg tcgaaaacca cttcgacacc tcttatgaat tagaatctct ccttgaacag
      121 cgcgtgaagc gtcaactgct ggcggaagta caatctatct gtccgccggg cgtgaccatt
      181 atgaacgtgc gtcagggtga acctttaggt ttaggccact ccattttgtg tgcacgcccc
      241 gccattggcg acaacccatt tgtcgtggtg ctgccggacg ttgttatcga tgatgccagc
      301 gccgacccgt tgcgttacaa ccttgctgcc atgattgcgc gcttcaatga aacgggacgc
      361 agccaggtgc tggcaaaacg tatgccgggt gacctctctg aatactccgt catccagacc
      421 aaagaaccac tggatcgcga aggtaaagtc agccgcattg ttgaatttat cgaaaaaccg
      481 gatcagccgc agacgctgga ctcagacatc atggccgttg gtcgctatgt gctttctgcc
      541 gatatttggt cggaacttga acgcactcaa cctggtgcat gggggcgtat tcagctgact
      601 gatgctattg ccgaactggc gaaaaaacag tccgttgatg ccatgctgat gactggtgac
      661 agctacgact gtggtaaaaa aatgggctat atgcaggcat tcgtgaagta tggattacgc
      721 aacctgaaag aaggggcgaa gttccgtaaa gggattgaga agctgttaag cgaataatga
      781 aaatctgacc ggatgtaacg gttaataaga aaattataac ggcagtgagg attcgtggcg
      841 aaagtaattt gttgcgaata ttcctgccgt tgttttatat aaacaatcag aataacaacg
      901 agttagcaat aggattttag tcaaagtttt ccaggatttt ccttgtttcc agagcggatt
      961 ggtaagacaa ttagcttttg aatttttcgg gtttagcgcg agtgggtaac gctcgtcaca
     1021 tcgtaggcat gcatgcagtg ctctggtagc tgtaaagcca ggggcggtag cgtgcattaa
     1081 tacttctatt aatcaaactg agagccgctt atttcacagc atgctctgaa gcaatatgga
     1141 ataaattagg tgaaaatact tgttactggt ggcgcaggat ttattggttc tgctgtagtt
     1201 cgtcacatta taaataatac gcaggatagt gttgttaatg tcgataaatt aacgtacgcc
     1261 ggaaacctgg aatcacttgc tgatgtttct gattctgaac gctatgtttt tgaacatgcg
     1321 gatatttgcg atgcagctgc aatggcacgg atttttgctc agcatcagcc agatgcagtg
     1381 atgcacctgg ctgctgaaag ccatgttgac cgttcaatta caggtcctgc ggcatttatt
     1441 gaaaccaata ttgttggtac atatgtcctt ttggaagccg ctcgcaatta ttggtctgct
     1501 cttgatagcg acaagaaaac tagcttccgt tttcatcata tttctactga cgaagtctat
     1561 ggtgatttgc ctcatcctga cgaggtaaat aatacagaag aattaccctt atttacagag
     1621 acaacagctt acgcgccaag cagcccttat tccgcttcaa aagcatccag cgatcattta
     1681 gtccgcgcgt ggaaacgtac ctatggttta ccaaccattg tgactaattg ctctaacaat
     1741 tatggtcctt atcatttccc ggaaaaattg attccattgg ttattctcaa tgctctggaa
     1801 ggtaaggcat tacctattta tggcaaaggg gatcaaattc gtgactggct gtatgttgaa
     1861 gatcatgcgc gtgcgttata taccgtcgta accgaaggta aagcgggtga aacttataac
     1921 attggtggac acaacgaaaa gaaaaacatc gatgtagtgc tcactatttg tgatttgctg
     1981 gatgagattg taccgaaaga gaaatcttac cgcgagcaaa ttacttatgt tgccgatcgc
     2041 ccgggacacg atcgtcgtta tgcgattgat gcagagaaga ttagccgcga attgggctgg
     2101 aaaccgcagg aaacgtttga gagcgggatt cggaagacag tggaatggta cctgtccaat
     2161 acaaaatggg ttgataatgt gaaaagtggt gcctatcaat cgtggattga acagaactat
     2221 gagggccgcc agtaatgaat atcctccttt tcggcaaaac agggcaggta ggttgggaac
     2281 tacagcgtgc tttggcacct ttaggtaatt tgattgctct tgatgttcac tccactgatt
     2341 attgtggtga ttttagtaac cctgaaggtg tagctgaaac agtcaaaaga attcgacctg
     2401 atgttattgt taatgctgcg gctcacaccg cagtagataa ggctgagtca gaacccgaat
     2461 ttgcacaatt actcaatgcg actagtgttg aatcaattgc aaaagcggct aatgaagttg
     2521 gggcttgggt aattcattac tcaactgact acgtattccc tggaaatggc gacacgccat
     2581 ggctggagac ggatgcaacc gcaccgctaa atgtttacgg tgaaaccaag ttagccggag
     2641 aaaaagcgtt acaggaacat tgcgcgaagc atcttatttt ccgtaccagc tgggtatacg
     2701 caggtaaagg aaataacttc gccaaaacga tgttgcgtct ggcaaaagag cgcgaagaac
     2761 tggctgtgat aaatgatcaa tttggtgcgc caacaggtgc tgagctgctg gctgattgta
     2821 cggcacatgc tattcgtgtg gcactgaata aaccggaagt cgcaggtttg taccatctgg
     2881 tagccagtgg taccacaacc tggcacgatt atgctgcgct ggtttttgaa gaggcgcgca
     2941 aagcaggtat tccccttgca ctcaacaagc tcaacgcagt accaacaact gcctatccta
     3001 caccagctcg tcgtccacat aactctcgcc ttaatacaga aaaatttcag cagaactttg
     3061 cgcttgtctt gcctgactgg caggttggcg tgaaacgaat gctcaacgaa ttatttacga
     3121 ctacagtaat ttaatagttt ttgcatcttg ttcgtgatga tggagcaaga tgaattaaaa
     3181 ggaatgatga aatgaaaacg cgtaaaggta ttattttagc gggtggttct ggtactcgtc
     3241 tttatcctgt gactatggct gtcagtaaac agctgttacc tatttatgat aaaccgatga
     3301 tctattaccc gctttctaca ctgatgttag cgggtattcg cgatattctg attatcagta
     3361 caccacagga tactcctcgt tttcaacaac tgctgggtga cgggggccag tgggggctaa
     3421 atcttcagta caaagtgcaa ccgagtccag atggacttgc acaggcattt atcatcggtg
     3481 aagagtttat cggaggtgat gattgtgctt tggttctagg tgataacatc ttttacggtc
     3541 acgacctgcc gaagttaatg gatgtcgctg ttaacaaaga aagtggtgca acggtatttg
     3601 cttatcacgt taatgatcct gaacgctatg gtgtcgttga gtttgataaa aaagggacgg
     3661 caattagtct ggaagaaaaa cctctacagc caaaaagtaa ttatgcggta actgggcttt
     3721 atttttatga taacgacgtt gtcgaaatgg cgaaaaacct taagccttct gcacgtggtg
     3781 aactggaaat taccgatatt aaccgtattt atatggaaca ggggcgttta tccgttgcca
     3841 tgatggggcg tggttatgcg tggctggaca cggggacaca tcaaagcctg attgaggcaa
     3901 gcaacttcat tgctacaatt gaagaacgtc aggggctgaa agtttcctgt ctagaagaaa
     3961 ttgcttaccg taaagggttt attgatgctg agcaagtaaa agtgttagca caacctctga
     4021 aaaaaaatgc ttatggtcag tacctactaa aaatgattga aggttattaa taaaatgaaa
     4081 gtaattaaaa ctgaaattcc tgatgtatta attttcgaac cgaaagtttt tggcgatgag
     4141 cgtggtttct ttatggaaag ctttaatcag aaagttttcg aagaagctgt agggcgtaag
     4201 gttgaatttg ttcaggataa ccattcgaag tctaataaag gcgttttacg cgggctgcat
     4261 tatcagttag aaccttatgc gcaaggaaaa ttagttcgct gtgttgttgg cgaggttttt
     4321 gatgttgctg tggatattcg taagtcatcc cctacgtttg gtaaatgggt tggtgtgaat
     4381 ttatcttctg agaatatgcg acagttatgg atacctgaag gatttgctca tgggtttttg
     4441 gtgctgagtg atactgcgga atttttatat aaaacgacga agtattacca tccagagagt
     4501 gacagtggta ttatttggaa tgataaaaat atcggaattt gttggccatt aagagaaaaa
     4561 ccaataatat cagaaaaaga tagcaatcta ttaccctttg ctcatttaac tcgcaaacaa
     4621 ataaatttat gaatgtttta atactcgatg caaatttaaa aaaacaaagc actatataaa
     4681 taatgatttt gataataggg ttagcattaa ttaaccctat tattacggaa agttttcggt
     4741 gtggcgtagg tacaatgata caaatggcag gaaaagatca cgtaatgctg tttttttgta
     4801 ctaagtaatt cgcattttat gtttaaaaat tgagatactc ctcattacct gaagctgttt
     4861 tattattgct tatacatgat caaatactcc ttacttaatt aaggagagca aaatggaaat
     4921 taaaaaattg atggaacata tttctattat tcccgattaa agacaatcct ggaaagtgga
     4981 acataaattg tcaaatattc cactattgac tatttgtgcc gttatttctg gtgcagaagg
     5041 atgggaagat atagaggatt ttggggaaac acatctcgat tttttgaagc aatatggtga
     5101 ttttgaaaat ggtattcctg ttcacgatat cattgccaga gttgtatcct gtatcagtcc
     5161 tgcaaaattt cacgagtgct ttattaactg gatgcgtgac tgccactcat caaatgataa
     5221 agacgtcatt gcaattgatg gaaaaacact ccggcactct tatgacaaga gttgtcgcag
     5281 gggagcgatt catgtcactc tagtgcgttc tcaacaatgc acagtctggt catcggacag
     5341 atcaggacgg atgagaaatc caatgaaatt acaactatcc ctgaacttct taacatgctg
     5401 gatattaaag gaaaaatcat cacaactgat gtgatgggtt gccagaaaga tattgcagag
     5461 aagatacaaa aacagggcgg tgattattta ttcgctgtaa aaggaaatca ggggcggctt
     5521 aataaagccc ttgaggaaaa atttccactg aaagaattaa ataatccaga acatgacagt
     5581 tacgcaatca gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgttttcgat
     5641 gtccctgatg aacttattga tttcacgttt gaatggaaag gactgaagaa attatgcgtg
     5701 gcagtctcct ttcggtcaat aatagcagaa caaaagaaag agccagaaat gacggtcaga
     5761 tattatatca gttctgctga tttaaccgca gaaaatttcg ccacagcgtt ctgaaatcac
     5821 tgagtccttg cgggtagcag gctttcataa tctttccctg gcgtagttgc taattatccc
     5881 agaaaattta cagagtaaaa atgttaatct tgggtaaaag agatgtatat taaccatatc
     5941 tctttttcaa atatttttta gagtaaaaaa tcttttcatg aaaaaaaata taatatatct
     6001 tttttttgtg cagggcacaa actatctgtt tccactaata acattgccat atttgattcg
     6061 catgttaggg agtaactctt ttggcatata tgcaatgata cttgctggaa tacagtatgt
     6121 taatataatt gttgatttcg gttttaactt cactgctaca aaactaatct caatatataa
     6181 aaataataaa aatgagaaaa ataaaatatt cactgcaaca attataatta aatttatttt
     6241 attctgtctt tgcctttctg ttctattatt attatctttg gtattagaac aggaaaaata
     6301 tatacaatat ataataatag gttctatcgc tgtaattggg aatatgctct tacctgtttg
     6361 gttttttcag ggaatagaga gaatgagtaa gattgcatta ttttctactt tatctaaact
     6421 ttcatcatta ctatttattt tttttacggt aaaatcatca gatgatgttg gactagctat
     6481 agcaagttac tcattaggct tgctatttac tgggctatta tcagtttatg ttatctttca
     6541 ttataaatat gttaaattta caaaggtgag tttaaagttt ataatcaaat tagctaaaga
     6601 tagtgttgat atgtttatat caaacattac tgttagtttt tatactacac ttaatacaat
     6661 tttagttggt gctattgctg gccctgcaac agcaggttac ttttcggctg cggataaatt
     6721 aaggatagcg gttcaaggac ttttaacacc tttgcagcaa gcaatttttc caagagttac
     6781 aagtttgatt aataatgact ataagctgaa tgatgtatta aaaacatttg gctataaaat
     6841 gattggcttt ggattttttc tatcactaag cattgcgatc attggagttc cattatccga
     6901 gtggtatttt ggtaacaata atgcaatatc atcaaaaata cttttaatac ttagtccact
     6961 tccttttgtc gtgtctattg gtattgtatt cgggcaatgg tggctaatta ccactaatag
     7021 gacaagaata atcagaaaag tatattttaa tttaagcatg ctacatgttt ttattgctta
     7081 tttcttaatt aagcacattc cgaattatgg cgtagcaata tcaatagttt ttactgaaac
     7141 tatcatatca ttattactta caatttttgc tataaaaaac tcagttaatc attgaaaaag
     7201 aggaaacgaa ttgaaagtta tagcatttta tttaccacaa tatcatgtga ttcctgaaaa
     7261 tgacaaatgg tggggagaag gttttacaga atggacaaat gttaaaagag cagtgcctct
     7321 gttttcaggt cataatcagc cacgaatacc acttaataat aattattata acttgttgga
     7381 caagaataca ttgatttggc aagcaggtct ggccaatcag tataaaattg gtgcaatgtg
     7441 tttttatcat tattggtttt taggaaaaca actgcttgaa aaaccagcag agatattatt
     7501 agaaaataaa gaaattaata tgcctttttt attttcttgg gctaatgaac cttggacacg
     7561 tgcttgggat ggtagtcata gaaatgtgct tatgcctcag agttatggta cagaagctga
     7621 ttgggaaaaa cattttaatt atttaaagcc cttttttgaa gatgaacgtt acttaaagca
     7681 tgatggaaag ccgattttca ttatttatcg tactgctaac tttgcacttt gtaatgagtg
     7741 gatctcatgt tggagagagt tatcgaagaa tacgagtttc aaagatatac attttgttag
     7801 tagttataca tcgtttgaaa atgacccaag aaagctggat tttgatgctc atttatattt
     7861 tgaacctatg tgtaccgttg gacataactt aggttacttt aataagaaaa taaaaaaaat
     7921 aaatgcaaga ttaaagagag taattaaccc aaaagtttgg acgacatatg ttgaaaatac
     7981 tttgcaatat gattacttat gggaaaagat tttgaagaaa aaaatcaata aaacacaata
     8041 tcctggagca tttgtagact gggataatag tgcccgaaaa aaatctagag cattagttat
     8101 tcatggtggt tcacctaaaa aatttggact ttatttagat aagttatata aaagaagcat
     8161 tgaaaataat tgtccgtttc ttttcattaa tgcctggaat gaatgggctg aagggacata
     8221 tctggagcca gatgaaaaaa ataaatactc gtatttagaa gaattaaaaa aagtcattga
     8281 aaaatatgag gtatagtagg ttataaaaat gacggaattg gttgaattag gtaggagaat
     8341 aatttgaaat tatatcgccc gataaaaaaa tatttggtca tgtatatttt tatactattg
     8401 gctttatggg tatgccaaac aagtataaat tataaaataa attatgggtt tatttatgtg
     8461 ctgttatttt atagtttctt tattatctcc ttgttttttt ttccatcgag agtaaaaaaa
     8521 gaacctaaag cttatttaaa tggcggcgct aacaaaatta aaaaaattgt taaaatatta
     8581 tcttatataa atttaataat attagcatac ttactctacc aattttacaa tatattacaa
     8641 tctggtattg caatatcagt atatagaagt agtttatttg gagatagttc tataaaagac
     8701 tactatggaa atgcagcagc atatatgttt ttctattgta caattgtttc attactaaca
     8761 ataagctttg gcatttattg gaagatatat acagcggaaa attcaatatt attgataggg
     8821 ttattatttc ttatcatgaa ggaaatagtg cttgtatcta ggttttattt attcccaacc
     8881 ttcttatcat taatgcttat actttattgc tttgataaaa agattacgcg aaagcgtata
     8941 tcaatctaca tgtttttatt attaatgcca attatatttg cttttttact aaaaagcaat
     9001 ggttcattag atacagtatt ttataatgcc agaaattact ttgtagtggg gttttcatta
     9061 tttacaaatg caattgattc agaacttacg gatcagctac attatatggg aagccctttt
     9121 gcttttttgg gcgttttcag cacatttttt tataataaaa atttgttctt tgaaaaaatt
     9181 caagaatttg tctctctagg tgatgctggt tattttaatg ctttttacac atcgtttttg
     9241 ctgccatact tatattttgg atttattgga actataatat gtagcatatt tttcggactg
     9301 ctaatttctt ataatttata tcggttaacc ataactcaat cgttttttaa ttttaatact
     9361 ctatttgcat cccttcccat ttttttcagc caacaattta ccccagtaca attgaccttt
     9421 ttttgggatt atttttattt atcaatagtt gctcagttcg tcagacgatt gtgtttacgt
     9481 aaaatctgag gtttatacag tgaaaaatgc tactatatta gttttataca accctaatat
     9541 taaatatgtt attaataaca taaatatcat atctgagtta actgacttac ttattttaat
     9601 cgataatact cctgagcatg aaaagagaga aattaacaaa aaaatactac tccagtctat
     9661 aagtggtttt gacgaagttt attatatagg aatgaataat gaggggttgt ctaaagctta
     9721 taatgaagga gtaaaaaagc taaactcatg ttgccataaa attggtgggg tactgtttct
     9781 tgatcaagac agtgatgtat ctcatgcaac attaaagcaa ttgtgggata cttataataa
     9841 gttagaggac atctgtgact taggggtttt gggcgcgtat ccaattaggg ttgatggcat
     9901 aggttataga tataatgtaa aaaaagaaaa cactaatata ctgcctacaa acgttataga
     9961 agtaaaacgt gtaattagta gcttttcaat agttccactg agagtattaa atgagttaaa
    10021 tgggttttat gatgattttt ttatcgacca tattgataac gattttagtc gacgctgtat
    10081 aaagaaaaat aagattgtcg cgatggattt gaatgcgaaa tttatacagc gtataggtga
    10141 aggctcgatt acttttttag gaaagcatat tacaccaata tccgcacctt ttagacatta
    10201 ctatcaggca agaaatattt tattaagcgc taagcgttgt ggtgatggaa taatatatgc
    10261 cttaccatat cttgtgaaaa gatttatcat gatcaatctt caaggtgttt atgggcataa
    10321 tttaattaca agatataagt tttatttgtt aggtatattg catgggatta ataatagatc
    10381 aggaaaattg aaatgaaaat ttgttatgtt ataacaaagg ctgatgagat aggtggagct
    10441 caaattcacg tgcgtgattt gtctatacaa ttagctaaag ataataacga ggtttttgta
    10501 atagtgggtg aaaatggtgc acttgtagat gaattagtaa ataataatat ttctgtctac
    10561 gtattgccta ccttagtcag acaaatcaat cccattaaag atttccaatc aatcctaaaa
    10621 cttcctaaaa tcataaagaa aaataaacca gatggctggt cattacattc ttcaaaggct
    10681 ggaatcgttg gccgtttggc tgcaataggt actgatattc ctgtaatatt tactgctcat
    10741 ggatgggctt ttgcgaatgg tgttggaaaa agacaacaga aattatactg tgctattgaa
    10801 aaaattatgt ctccatttgc aaaaaaaatt atcactgttt ctcagcaaga taaagactta
    10861 gcaattaaat atcgagttgt ttcagctgaa aaacaagttg ttatacataa tggaatgcct
    10921 gaaattgaaa tggtttgtga taataaaaaa attgaatctt atcctacaat caagctcata
    10981 tcagttgctc gtttttcaga acagaaagat cataaaacac tttttttagc gttaaaagaa
    11041 cttaaagatt atgattggtc tcttagcttg gtcggtaaag gaccattaat agaccactat
    11101 aaaaaatatg caattgaact tggtatcttc gaacgtatta aatttcttgg agagagaagc
    11161 gatgttgcgg atttattatc tgagagcaat gtatttttgc ttatttctaa ctgggaaggt
    11221 tttcctcgta gtattcttga agcaatgaga gcaggcttac cagtaattgc atctgatgta
    11281 ggcgggacat cggaatcagt tcttaataat aaaacagggt ttcttatttc tcgaaatgat
    11341 gtccatactt taaaagaaaa gattgaaatc ttgattaaaa atgaagcact tcgtaatgaa
    11401 atggggcggt atgctagaga aatttatctc gcaaacttta cttttgattc tatgtataat
    11461 aagacagtag cattatataa atctttagtt aatatttcat aaatttatct aatattaatt
    11521 ttttaatgtg aagcatgtat gcccatacgt acatcatata tttgataatt cactgacagg
    11581 agtaaacaat gtcaaagcaa cagatcggtg tcgtcggtat ggcagtgatg gggcgcaacc
    11641 ttgcgctcaa catcgaaagc cgtggttata ccgtctctat tttcaaccgt tcccatgaga
    11701 agacggaaga agtgattgcc gaaaatccag gcaagaaact ggttccttac tatacggtga
    11761 aagaatttgt tgaatctttg gaaacgcctc gtcgcatcct gttaatggtg aaagcaggtg
    11821 caggcacgga tgctgctatt gattccctta agccatacct agataaaggt gacatcatta
    11881 ttgatggtgg taataccttc ttccaggaca ccattcgtcg taaccgtgag ctttctgccg
    11941 aaggctttaa cttcattggt accggtgttt ccggaggcga agaaggcgcg ttgaaaggtc
    12001 cttccattat gcctggtggc cagaaagaag cctatgaact ggttgcacca atcctgacca
    12061 aaatcgccgc agtagctgaa gacggtgagc catgcgttac ctatattggg
//
