LOCUS       EU296418               13237 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella boydii type 2 O-antigen gene cluster, complete sequence.
ACCESSION   EU296418
VERSION     EU296418.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="type: 2"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACD37121.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEENYEGRQ"
     misc_feature    1137..12439
                     /note="O-antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACD37122.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGMYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPATAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4063
                     /gene="rmlA"
     CDS             3179..4063
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACD37123.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVDKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKILAEPL
                     KKNAYGQYLLNIINNKIS"
     gene            4106..5353
                     /gene="wzx"
     CDS             4106..5353
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37124.1"
                     /translation="MKLVTSLNKNIFYLAILQGSYFILPLLTFPYLVRVLGPYSFGLL
                     GFCQATMQYLVMVTDYGFNWSATQDVSKSKNDKEKLTTIFWNVFFSKIILALSAIVIF
                     FGITFFIKELYYAQVVLISFIPMVLGNILYPVWFFQGLEKMKWITLCSLSARLLLIPL
                     TFLLVKNPQDIWLAALIQGGVNFIAGLIGFAIIINNNWIGKLVFDYDGIIYSLKSGWH
                     IFISTAAISLYTTTTVVVLGFICGPVSVGYFNAANTIRNAAQSLMNPIYQAVYPRINS
                     LFEENYAKSMNLLKKSFKGVLFISFSGSLLLCILSPIIIRYGVGDKYTESISILRILA
                     FLPFIISLSNFFGIQTMLTHGYKRQFSKILLICGVMHVIIIAPLIFFAGAYGAAMTVL
                     ITETVVASLMYLFLKRKKINLFK"
     gene            5366..6130
                     /gene="wfdA"
     CDS             5366..6130
                     /gene="wfdA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37125.1"
                     /translation="MALFIACHKQTELPNHECYIPIHAGKKISGKMLDQVLGDDTGNN
                     ISCKNKNYCELTVIYWLWKNKLFNDDYIGLVHYRRYFGNVLSNFKFKEVRIYDRANIS
                     NKMKQYDIIIPVKESLKLSVVEHYRKFHNVEDLMLAKKIVDKLYPDYSVAFRELLEQK
                     KISLYNMFIMKAGIFDCYCDWLFSILDELEKEINLRRYDEYQSRVFGFIAERLLNVWI
                     NKNKEVFKVGEEFVINTDECSGRGAQLISQLRAKLK"
     gene            6127..7314
                     /gene="wzy"
     CDS             6127..7314
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD37126.1"
                     /translation="MNMTIFIAIIMLMYSGLLLSINKDIKSPSAILFFIWGGLLFLSG
                     VNGDITNYTLCIILFSCLSFSVGALLAKPYAPIFEHVFSYNINNTKALVLITNIISFV
                     ILIYTLIVFLYYYKGGFTESYINSRTEINYGDKSGLIKIYGYIYYLLYPLVYVWSFLY
                     FKNKYKKIEEGERARLPYNNRTFYFVFFTSLFYALLSTAKIKVLLLIIPIVFLRLFFQ
                     KISLKYLLVTVSGIIFFFYLSMLFLNKIDDSSGAFEALKYGLMNYSLANIFALDSVLQ
                     GKAVVIDCNGDATCGLANFISYKEYKTNVFTIFYSLTKYSDFLYSLIFFFVIGFFHSS
                     LYNVAKKNKKTISVVICSILYFPLLFQFFDQLYMLMFYIYAIAMLYVICFCSRLTLTL
                     KGV"
     gene            7316..7948
                     /gene="wfdB"
     CDS             7316..7948
                     /gene="wfdB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37127.1"
                     /translation="MLEHIVVFDVCGTLYLSNTTFDYVVFVHRKQKKYTKLIKCLLLR
                     SLFGKLLHKLRICSLRKHFLKTLEGYEKSTLEELANLFYDEFLIFKEKNEVMALLERY
                     KHEKIILMSASVDPVINVIAKNLNVEAVSSKLEFIDNKSTGLLEYNLTGIKHLQVNNP
                     VSLIVTDNFSDINLIKLADTAVIISKAKDKNKWRVLLNRFGVSKDKVRFM"
     gene            7945..8754
                     /gene="wfdC"
     CDS             7945..8754
                     /gene="wfdC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37128.1"
                     /translation="MILYFPLSYLFISRLKTWHEKISWFIIYPGFLLLCCFLYNGSFV
                     SLLLSFMMTMSIYEIGYLDNDYRTINTEKAPTIRGGELRDTIKSKLSSIIFIRVVYFF
                     VSFALLIYQSDIIAHHYTVIYFVLCIGFLIISFYLHNTIRDKRNIITYFSLVSCRYII
                     PLIPTVTCSIFFVFVILLLFPLIRTLEHACKEKYGIKRLKDFIIYPDLFRVKYYFFFF
                     DAAIVYAYIYQDWKIVLLSGYFLFYRYITFKVSDKNFVKRTHHGSYKWNKK"
     gene            8751..9623
                     /gene="wfdD"
     CDS             8751..9623
                     /gene="wfdD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37129.1"
                     /translation="MNRVAVIIVLYYPDVSKINKMIDSLGKKQREILLVDNTPELHLE
                     SDVLCNNNIHYYHLGDNKGIAFAQNYGFIKAIGLGKPLFFTFDQDSCISKDYISSMLK
                     EYEYACSLRSNIAALGPTIINERNGKKYDREIKLGSQISETIHDVESIISSGALIPLE
                     AIINIGLNKVSWFIDLIDIEWSFRARKEGWNILVTDKVLISHNIGSKDINIMGIKTFT
                     VCSPFRLYYVYRNWLLALREPVFPLRYKLKKLITMPVRIIIYAFCERGGQRLNYIFKG
                     IKDGLLGRKGSFKG"
     gene            9586..10755
                     /gene="wfdE"
     CDS             9586..10755
                     /gene="wfdE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37130.1"
                     /translation="MDCLDEKDHLRDNHSLKIVLTANSSWYIYNFRRSTIKALINIGF
                     DVLVVVPDYEYKDSILKFGAKFEKVDLKPKSINIFTECTSLISYYKIIKKFNPQVILT
                     FTPKANIYCGLLAYKFDAKVIPNISGLGSAFVNSNTILSQIVKLLYKIALKKAAFVFF
                     QNEDDRQLLINSGCVNYEKTCRIFGSGVDLSKFLPSNKNLKQRSCVKFILVARLLYTK
                     GILHYLKAAEIIKSKYPNCSFSLLGAFEKNEKIITKELIEGFCTRGIVNYYGTSDDVA
                     SIMKHYDAIVLPSFYREGVPKALIEGASSGLAILTTDNVGCRDMVQNEVNGFLCQPND
                     LDSLVDIIEKYILLTDEMKNTMSIKSREFAESHCDENKIIEKYTNTINDVLNRNH"
     gene            10770..11300
                     /gene="rmlC"
     CDS             10770..11300
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACD37131.1"
                     /translation="MKVQDTVLDGVKIIEPVVYGDNRGFFLEVYQKQRYQNLLNIEFD
                     FVQDNYSRSSKNVLRGLHFQKTKPQGKLVRVVRGEVFDVAVDIRKESPNYGQWIGVLL
                     SEMNKKQFWVPPGFAHGFVVLSEMADFEYKCTEYYDPEDECCLLWNDPELNIQWPLSN
                     PILSDKDMKGCLLKEL"
     gene            11333..12439
                     /gene="glf"
     CDS             11333..12439
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37132.1"
                     /translation="MQYDFIIIGSGLFGAVCAYELNKRNKRVLVIEKRDHIGGNIYTE
                     KVDDIHIHKYGAHIFHTNDQYIWNYINKFASFNRFTNSPLAYHKGKLYNLPFNMNTFY
                     QLWGTKTPSEAQDKIDLQRSKYSNITPTNLEEQALSLVGEDVYEKLIKGYTEKQWGRS
                     CSELPPFIIKRIPVRFTFDNNYFTDRFQGVPIGGYTQIIDKMLNGVDLLLNVDYLSEK
                     DKYNSMAERIIYTGPIDAYFDKKLGTLEYRSLKFITKKIEEANYQGNAVINYTEKDIP
                     FTRIIEHKHFDDSYETEYTFITEEYPIEWSEGQEPYYPINDKKNMDIYHKYRELSQME
                     SKVIFGGRLAEYKYYDMHQVIRSALNCVNKILHD"
BASE COUNT     4201 a   2064 c   2671 g   4301 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtaaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgttacaacc ttgctgccat gattgcacgt ttcaatgaaa cgggccgcag tcaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agaaccactg
      421 gatcgcgaag gtaaagtcag ccgcatcgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acgccggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaactggaac gtactcagcc tggtgcatgg ggacgtattc agctgactga tgccattgct
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaagcgttc gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ttgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagga taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtagacatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgac tctgaacgct atgtttttga acatgctgat atttgcgatg
     1321 ctgctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg
     1381 ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagcggctc gcaattactg gtctgctctt gatggcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc
     1561 atcctgacga agtaaataat aaagaacaat tacccctctt tactgagacg acagcttacg
     1621 cgcctagtag tccttattcc gcatcaaaag catccagcga tcatttagtc cgtgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc
     1741 actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatat cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac
     1981 cgaaagagaa atcttaccgt gagcaaatta cttatgttgc cgatcgcccg ggacacgatc
     2041 gccgttatgc gattgatgca gagaagatta gccgcgaatt gggatggaaa ccacaggaaa
     2101 cgtttgagag cgggattcgg aagacagtgg aatggtacct gtccaataca aaatgggttg
     2161 ataatgtgaa aagtggtgcc tatcaatcgt ggattgaaga gaactatgag ggccgccagt
     2221 aatgaatatc ctcctttttg gcaaaacagg gcaggtaggc tgggaactac agcgtgctct
     2281 cgcacctttg ggtaatttga ttgctcttga tgttcactcc actgattatt gtggtgattt
     2341 tagtaatcct gaaggtgtgg ctgaaaccgt caaaaaaatt cgccctgatg taattgttaa
     2401 tgctgcggct cataccgcag tagataaggc tgagtcagaa cccgaatttg cacaattact
     2461 caatgcgacc agcgttgaat caattgccaa agcggctaat gaagttgggg cctgggtaat
     2521 tcattactca actgactacg tatttccggg aaccggtgaa ataccatggc tggagacgga
     2581 tgcaaccgca ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcgttaca
     2641 ggaacattgc gcgaagcatc ttattttccg taccagctgg gtatacgcag gtaaaggaaa
     2701 taacttcgcc aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgattaa
     2761 tgatcaattt ggtgcgccaa caggtgctga gctgctggca gattgtacgg cacatgctat
     2821 tcgtgtggca ctgaataaac cagaagtcgc aggcatgtac catctggtag ccagtggtac
     2881 cacaacctgg cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggtattcc
     2941 ccttgcactc aacaagctca acgcagtacc agcaacagcc tatcctacac cagctcgtcg
     3001 tccacataac tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgttttgcc
     3061 tgactggcag gttggcgtga aacgaatgct caacgaatta tttacgacta cagcaattta
     3121 atagtttttg catcttgttc gtgatgatgg agcaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac
     3241 tatggctgtc agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct
     3301 ctctacactg atgttggcgg gtattcgcga tattctgatt attagtacgc cacaggatac
     3361 tcctcgtttt caacaactgc tgggtgacgg tagccagtgg gggctaaatc ttcagtacaa
     3421 agtgcaaccg actccagatg ggcttgcgca ggcgtttatt atcggtgaag agtttatcgg
     3481 tggtgatgat tgtgctttgg ttcttggtga taatatcttc tacggccacg atctgccgaa
     3541 gttaatggat accgctgttg acaaagaaag tggtgcaacg gtatttgcct atcacgttaa
     3601 tgatcctgaa cgctacggtg tcgttgagtt tgataaaaac ggtacggcaa taagcctgga
     3661 agaaaaaccg ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa
     3721 cgacgttgtc gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tggaaattac
     3781 cgatattaac cgtatttata tggaacaggg gcgtttatcc gttgccatga tggggcgtgg
     3841 ttatgcgtgg ctggacacgg ggacacatca aagcctgatt gaggcaagca acttcattgc
     3901 tacaattgaa gaacgtcagg ggttgaaagt ttcctgcccg gaagaaattg cttaccgtaa
     3961 agggtttatt gattctgagc aggtgaaaat attagctgaa ccactgaaaa aaaatgctta
     4021 tggtcagtat ctgctaaata tcataaataa taaaatatca taatattaac tagttgcagc
     4081 tatataaata ggctcggaga ttgcagtgaa gcttgttacc tctcttaata aaaatatctt
     4141 ctatttagct atattgcaag gaagttattt tatactgcca ttgttaacat ttccttatct
     4201 tgttagagta ttaggaccat actcttttgg tctattaggg ttttgtcaag caacaatgca
     4261 atatcttgtg atggtgactg attatgggtt taattggtca gcaacacaag atgtttcgaa
     4321 aagtaagaat gacaaggaaa aattaacaac aatattttgg aatgtcttct ttagtaagat
     4381 tatacttgca ctttcggcaa ttgttatatt tttcggaata acatttttta ttaaggagct
     4441 atattatgct caagtggtgt taatatcatt tattccaatg gtgttaggta atatattata
     4501 ccctgtttgg tttttccagg gattagaaaa aatgaaatgg attacattat gtagtttaag
     4561 tgcaagatta ttattaatac cattaacttt tttgttagta aaaaatccac aagatatctg
     4621 gttagccgct ttgattcaag gcggcgttaa ttttatagca ggtttaattg gatttgctat
     4681 tattataaat aataactgga ttgggaaatt ggtatttgat tatgatggta taatttattc
     4741 attaaaaagc ggatggcata ttttcatttc aacagctgct attagcttat atacaacaac
     4801 aactgttgtt gttcttgggt ttatatgtgg acctgtctct gttggctact ttaacgctgc
     4861 taatacaatt agaaatgcag cccagagtct aatgaatcct atttatcaag ctgtttatcc
     4921 aagaattaat tcattgtttg aagaaaatta tgcaaaaagt atgaatcttc tgaaaaaatc
     4981 atttaagggc gtattattca tttcgttttc aggttcatta ctcctgtgca tactttctcc
     5041 aataataata agatatggag tcggagataa atatactgag tcaattagta ttttaagaat
     5101 attagccttt ttgccattta ttatttctct tagtaatttt tttggcattc aaaccatgct
     5161 gacacatggt tataaaagac aatttagtaa aatactgcta atttgtggtg ttatgcatgt
     5221 gataatcatt gctcctttga tcttttttgc tggggcgtat ggggctgcga tgacggtttt
     5281 aattacagaa accgtagttg catcattgat gtatcttttc ttaaaaagaa agaaaataaa
     5341 tttgtttaaa taaagaggaa tattaatggc tttattcatt gcttgccata aacaaactga
     5401 attacctaat catgagtgct atatccctat tcatgcgggt aagaaaatat cagggaaaat
     5461 gcttgatcaa gtgttgggag atgatacagg gaataatata tcatgtaaaa ataaaaatta
     5521 ttgtgagctg accgttatat attggctttg gaaaaataaa ctttttaatg atgattatat
     5581 tggcttggtt cattatagga ggtattttgg aaatgtttta tcgaatttta aatttaaaga
     5641 ggtgaggata tatgacagag ctaatatttc taataaaatg aaacaatatg atataattat
     5701 tccggtgaaa gagagtttga agctatctgt tgttgaacac tacaggaaat tccataatgt
     5761 cgaagatcta atgttggcaa aaaaaatagt tgataaatta tatcctgatt atagtgttgc
     5821 attcagagaa cttttagaac aaaaaaaaat atcgctttat aatatgttta taatgaaggc
     5881 tgggattttt gattgttatt gtgattggtt gttttccata ttagatgagc tggagaaaga
     5941 aataaattta cgcagatacg atgaatatca atcacgtgtg ttcggcttta ttgctgagcg
     6001 acttctgaat gtatggatta ataaaaataa agaagttttt aaagtcggtg aggaatttgt
     6061 aattaacact gatgaatgca gtggaagagg agctcaatta atttctcaac ttagagctaa
     6121 gcttaaatga acatgaccat ttttatagct ataataatgc ttatgtattc aggattatta
     6181 ctgagtatta ataaagatat taaatcgccg tcggcgattc tattctttat ctgggggggg
     6241 ctgttatttt tgtcgggagt aaatggagac ataactaact atacattatg tataatttta
     6301 ttttcatgtt tatctttttc ggtaggggct ctactggcaa aaccttacgc tcctatattt
     6361 gaacatgtat tttcctataa tataaacaat acaaaagcgt tagtattaat tacaaatatt
     6421 atctcgtttg ttattttaat ctatacttta atagtttttt tatattatta taaggggggc
     6481 ttcactgaat cctatataaa ttcacgtaca gaaattaatt acggagataa atcgggatta
     6541 ataaaaatat atgggtatat atactatctt ttatacccat tggtatatgt atggagcttt
     6601 ttatatttta aaaataaata taaaaagatt gaagaaggag agcgggcccg gttaccatat
     6661 aataatcgaa cattttattt tgtttttttt acttcacttt tttatgcgtt attatcaaca
     6721 gctaaaatta aagttttatt actaattata ccaatagtgt ttttgcgatt attttttcaa
     6781 aaaatatctt taaaatattt acttgtcaca gtgtctggta ttatattttt tttctattta
     6841 tctatgttgt ttttaaataa aattgacgac tcttcaggag cattcgaagc gttaaaatat
     6901 ggattgatga actatagctt ggcaaacatt tttgcattag attcggtttt gcaaggtaaa
     6961 gcagtagtta tagattgtaa tggagatgct acatgtggat tagctaattt tatatcatat
     7021 aaagagtata agacaaatgt ttttacaata ttttattcgc taacaaaata ttctgatttt
     7081 ttgtactcat taattttctt ttttgtcata ggtttctttc attcatcttt gtacaatgta
     7141 gcaaaaaaaa ataagaaaac gatatcagtt gtcatttgct ctattttgta cttccccttg
     7201 ttatttcaat tttttgacca attatatatg ttgatgttct atatttatgc aattgcaatg
     7261 ctttatgtga tatgtttttg ctcaagatta actctaactt tgaagggagt ttaacatgct
     7321 cgaacatatt gttgtatttg atgtatgtgg cactctttat ttgtcgaata caacttttga
     7381 ttatgttgta tttgtacata ggaaacaaaa aaaatatacc aagttaatta aatgcttatt
     7441 attacgctcc ttatttggaa agttgctaca taaattgcgt atatgttcct tacgaaaaca
     7501 ttttttgaag acattagaag gatatgaaaa gtctactttg gaagagttgg cgaatttgtt
     7561 ttatgatgaa tttctcattt ttaaagaaaa gaatgaagtt atggcacttt tagaacgata
     7621 taaacatgag aagatcattt taatgtctgc ttccgttgat cctgtcataa atgtaattgc
     7681 taaaaatttg aatgttgagg cagtatcatc taaattagaa tttatcgaca ataaatcaac
     7741 agggttatta gaatataatc ttactggtat aaagcattta caagttaata accctgtaag
     7801 tttgattgtc actgataatt tcagtgatat taatcttatt aaacttgcag atacagcagt
     7861 gattatttca aaagccaaag ataaaaataa atggagagta ctattaaata gatttggtgt
     7921 ctccaaagat aaagtgaggt ttatgtgata ttgtatttcc cattatcata tttattcatt
     7981 tcaagattga aaacatggca tgaaaaaatt agctggttta ttatatatcc agggtttcta
     8041 ttgctgtgtt gttttttgta taatggtagt tttgtatctt tgcttctgtc atttatgatg
     8101 accatgtcca tttatgaaat aggctactta gacaatgact atagaacaat aaatacagaa
     8161 aaagcaccaa caataagagg tggagagctg cgtgatacaa ttaaaagtaa attatcaagt
     8221 ataattttca ttagagttgt ttattttttt gtatcattcg cgttattaat ttatcaaagt
     8281 gatatcattg ctcatcacta tactgttatc tattttgtat tatgtatagg gtttttaatt
     8341 atctcctttt atttgcataa tacaatccgg gataagagaa atataatcac ttatttttct
     8401 ttggtgtcat gtcgttacat tattcctttg atacccactg tcacatgcag tatatttttt
     8461 gtttttgtca tattattatt attcccttta atcagaacat tagaacacgc ctgtaaggag
     8521 aaatatggta ttaaaaggtt aaaggatttt attatttacc ctgatttatt tagggttaaa
     8581 tattactttt tcttttttga tgctgctatt gtgtatgcat atatatatca ggactggaag
     8641 attgtattat tgtcgggata ttttttgttt tatcgataca taacctttaa agtatccgat
     8701 aaaaattttg taaaaaggac tcatcatgga tcttataagt ggaataaaaa atgaatagag
     8761 tagcggtgat aattgtacta tattatcctg atgttagtaa aatcaataaa atgattgact
     8821 cactgggaaa aaaacagcgc gaaatattac ttgttgacaa tacacctgag ctccacttag
     8881 aaagtgatgt tttgtgtaat aacaacattc attactatca tttaggagat aacaaaggca
     8941 ttgcgtttgc gcagaactat ggtttcatta aagctatcgg attaggaaag cccttattct
     9001 ttacatttga ccaagattca tgtatatcaa aagattatat atcatcaatg ttaaaagagt
     9061 atgagtacgc ttgctctctc cggagtaata ttgctgcatt aggtccaaca ataataaatg
     9121 aacgtaatgg gaaaaagtat gatcgtgaaa taaagctggg ctcccagata agtgaaacga
     9181 tacatgatgt tgaaagtata atatcttctg gtgcactcat acccctagag gcaattatca
     9241 atattggatt aaataaagta tcttggttta tcgatttaat tgacatagaa tggagtttca
     9301 gagctaggaa agaaggctgg aatatcctag tgacagataa agtccttatt tctcacaata
     9361 taggatctaa agatataaat attatgggga ttaaaacatt tacagtttgc agtccatttc
     9421 gtttgtatta tgtttataga aattggttat tagctctgag agagcctgta tttcctttaa
     9481 gatataagtt aaaaaaattg ataacaatgc ctgtccgaat cattatttac gctttttgtg
     9541 aacgtggagg acaacgatta aattatattt ttaaggggat aaaagatgga ctgcttggac
     9601 gaaaaggatc atttaaggga taatcattcc ttaaaaatag ttttaactgc taatagtagt
     9661 tggtatatat ataattttag acgaagcaca attaaggcac ttattaatat agggtttgat
     9721 gtattagtgg ttgtaccgga ttatgaatat aaggattcta ttttaaaatt cggcgctaaa
     9781 tttgagaagg ttgatcttaa accaaaatct attaatattt ttactgaatg tacatctcta
     9841 atatcatatt ataaaattat aaaaaaattc aatccacagg ttatattaac gtttacaccc
     9901 aaagctaata tatattgtgg tttgcttgct tataaatttg acgcgaaagt gatacctaat
     9961 atttctggcc ttggaagtgc atttgttaat agtaatacga tattaagtca aattgttaag
    10021 ctattatata agatagcatt aaagaaagca gcattcgttt tttttcaaaa cgaggatgat
    10081 cgtcagttat tgattaattc tggctgcgtc aattatgaga aaacatgccg aatatttggg
    10141 tctggtgttg atttatcaaa atttcttccc tcaaataaga atttaaagca aagatcatgt
    10201 gtgaaattca tacttgttgc tagacttcta tataccaaag gcatacttca ctatcttaag
    10261 gccgcagaaa taataaaatc caaatatcct aattgttcat tttcactgtt aggtgcattt
    10321 gaaaaaaatg aaaagataat cacgaaggaa ttgattgaag gtttttgtac gaggggtata
    10381 gttaattact atgggacttc cgatgacgtt gctagtataa tgaagcatta tgatgcaata
    10441 gtattaccat cattttatag agaaggtgta cctaaagcgt tgattgaagg tgcttctagt
    10501 gggttagcta ttttgacaac agataatgtt ggctgtaggg atatggtgca aaatgaagtt
    10561 aatggttttt tgtgtcaacc taatgatctt gatagcttag ttgacattat tgaaaagtat
    10621 attctactga cagacgaaat gaaaaacaca atgtctatta agtcgagaga atttgctgaa
    10681 tcacattgtg atgaaaataa gatcatagag aagtatacta atacaattaa tgacgtttta
    10741 aatagaaatc actaaaggtt ataataaata tgaaagttca agacactgtt ctggatggag
    10801 ttaaaattat tgaaccggtc gtatatggtg acaacagggg ttttttttta gaagtatatc
    10861 aaaaacaacg ttatcaaaat ttattaaata tagaatttga tttcgtacaa gataactatt
    10921 cacgatcaag caaaaatgtt ttaagaggat tacatttcca aaaaacgaag ccacaaggta
    10981 aacttgtaag agttgtgcgc ggcgaagttt ttgatgttgc cgtcgatatt agaaaagaat
    11041 cacctaatta tggccaatgg attggtgtgt tactttctga aatgaataaa aaacaatttt
    11101 gggtaccacc aggtttcgca catggttttg ttgtattatc tgaaatggcc gattttgaat
    11161 ataaatgcac agaatattat gatcctgagg atgagtgttg tttattatgg aatgatccag
    11221 aactcaatat tcaatggcct ttaagtaatc ctatattatc agataaagat atgaaaggtt
    11281 gcttacttaa agaactttaa ttttaactaa gttagatgac ataggaaccg caatgcaata
    11341 tgatttcata attataggtt caggtttatt tggtgcggtt tgcgcttatg aattaaataa
    11401 aagaaataaa cgtgtgttgg tcattgagaa aagggatcat attggaggga atatctatac
    11461 tgaaaaagtt gacgatattc acatacataa gtatggtgct catatatttc atacaaacga
    11521 tcaatatatc tggaattata taaataaatt cgcttctttt aatagattca ctaattctcc
    11581 attagcatat cataaaggta aattatataa cttacctttc aatatgaata ccttttatca
    11641 gttatggggg acaaaaactc cttctgaagc tcaagataaa atagatttac aaagaagtaa
    11701 atattctaac ataactccca caaatttaga agaacaagct ctttccctgg taggggaaga
    11761 tgtttatgaa aaactaatta aggggtatac ggaaaaacaa tggggacgtt catgttctga
    11821 attaccgcct tttattataa aaagaatccc agttcgattc actttcgata ataattattt
    11881 caccgataga tttcaggggg ttcctatcgg aggatacaca caaataattg ataagatgct
    11941 taatggtgtt gatcttctgc ttaatgttga ctatttatca gaaaaggata agtataattc
    12001 tatggcggaa cggattatat atactgggcc tatagatgct tattttgata aaaagttggg
    12061 aactttagaa tatagatcct taaagtttat aacaaaaaaa attgaggagg ctaattatca
    12121 aggcaatgcc gtaattaatt atactgaaaa agatattcct tttactagga taattgagca
    12181 taagcatttt gatgactctt atgaaactga atataccttt ataactgaag agtacccaat
    12241 agagtggagt gagggtcaag agccttatta tcccattaat gataaaaaaa atatggatat
    12301 atatcataaa tatagagagc tttcgcaaat ggagagtaaa gtaattttcg gaggacgcct
    12361 tgctgaatat aagtattatg atatgcacca agttatccgt tcagcactga attgtgtaaa
    12421 taaaatatta catgattaag ttgttttggc gttattaata ttattaatta aatatttaat
    12481 gtagtaaccc cctaacagga gtaaacaatg tcaaagcaac agatcggcgt cgtcggtatg
    12541 gcagtgatgg ggcgcaacct tgcgctcaac atcgaaagcc gtggttatac cgtctctatt
    12601 ttcaaccgtt cccgtgaaaa gacggaagaa gtgattgccg agaatccagg caagaaactg
    12661 gttccttact atacggtgaa agagtttgtt gaatctctgg aaacgcctcg tcgcatcctg
    12721 ttaatggtga aagcaggtgc aggcacggat gctgctattg attccctcaa accatatctc
    12781 gataaaggcg acatcatcat tgatggtggt aacaccttct tccaggacac cattcgtcgt
    12841 aatcgtgagc tttctgccga aggctttaac ttcattggta ccggtgtttc cggtggtgaa
    12901 gaaggtgcgc tgaaaggtcc ttccattatg cctggtgggc agaaagaagc ctatgaactg
    12961 gttgcaccga tcctcaccaa aatcgccgca gtggctgaag acggtgagcc atgcgttacc
    13021 tatattggtg ccgatggcgc gggtcactat gtaaaaatgg ttcacaacgg tattgaatac
    13081 ggtgatatgc agctgattgc tgaagcctac tctttgctta aaggtggctt gaacctttcc
    13141 aacgaagaac tggcgcagac ctttaccgag tggaataacg gtgaactgag cagctacctg
    13201 atcgacatca ccaaagatat cttcaccaaa aaagatg
//
