LOCUS       AF402312               12564 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Shigella boydii serotype 4 O-antigen gene cluster, partial
            sequence.
ACCESSION   AF402312
VERSION     AF402312.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Wang,L., Qu,W. and Reeves,P.R.
  TITLE     Sequence analysis of four Shigella boydii O-antigen loci:
            implication for Escherichia coli and Shigella relationships
  JOURNAL   Infect. Immun. 69 (11), 6923-6930 (2001)
   PUBMED   11598067
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Wang,L., Qu,W. and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2001) Department of Microbiology, The University
            of Sydney, Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="4"
                     /db_xref="taxon:621"
     gene            <1..765
                     /gene="galF"
     CDS             <1..765
                     /gene="galF"
                     /note="GalF"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glucose-1-phosphate
                     uridylyltransferase"
                     /protein_id="AAL27309.1"
                     /translation="IVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLA
                     EVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRY
                     NLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQ
                     TLDSDIMAVGRYVLSADIWPELEHTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSY
                     DCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AAL27310.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEENYEGRQ"
     gene            2223..3122
                     /gene="rmlD"
     CDS             2223..3122
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AAL27311.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNSE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWQIGVKRMLNELFTTTAI"
     gene            3180..4058
                     /gene="rmlA"
     CDS             3180..4058
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AAL27312.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSSRGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4063..4632
                     /gene="rmlC"
     CDS             4063..4632
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAL27313.1"
                     /translation="MNVIKTEISDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCAVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGLAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDPDIDVKWPLS
                     IHKPILSIKDEKQKMFKEMIALEKWNGEY"
     gene            4619..5461
                     /gene="wbdE"
     CDS             4619..5461
                     /gene="wbdE"
                     /note="WbdE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27314.1"
                     /translation="MENTNYYKLAILIVLYGKEVEQCETFTHIDRVLKKNRMSKDDII
                     LCLWNNGPREIAFNLTTDYYTLKVCQTINNESLSYIYNLFINEVESEKYILLDHDSIL
                     KYDYVLECLNSTSDCIIPNIIVGNNIQSPKTYSIQSFNKGNTVLAIGSGICISKKLCE
                     KLRQVFGDVFDSRFYFYGVDSTFFLRLNKIGESEFIKISECVVLHSLSRLENEAIKVK
                     QFRIKERSYDQGLTLRYYFCKFTFFTVAKKLLAKLIFNKSDISLIYFAKAYFSGKHYK
                     SGKS"
     gene            5458..6570
                     /gene="wbdF"
     CDS             5458..6570
                     /gene="wbdF"
                     /note="WbdF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27315.1"
                     /translation="MKFIIIGYRDAYFWNEYGTSVRDLQIAEILSRKYHVTFINRPVS
                     IYERLKNKKKAKSEYKAYSNRITFIDHTSYDLIGPLFGRIWTKYCYKKILNRISSLFT
                     DDSIIIDFTPIAELEFKNNKRITYWYDLIDNFSIHNRFSEKEKKAVRDKYAYVDENAD
                     IITAVSDKAIKNFLNPNKYVMPNGVYLENQNHECFIDGTPNKVYQLGFVGFVTDKLDI
                     DFLNKLSKKFSIIIWGKFFDDSIRDKLNENIKIAGAFKYSDLPSIMKSFKVGLLPYLP
                     EKSHDESPLKMYEYFKYGIPCLSSMNFEIKSRWFVNYLEYSKRDLLYEKINELIVSNN
                     SESIKASIQKEWLFEIKVNKIIEDIIRRKECGKKNS"
     gene            6551..7780
                     /gene="wzx"
     CDS             6551..7780
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAL27316.1"
                     /translation="MVKKIVNQFIYQFGNYILPFLIIPFLINKLSIAVYKDFVVIQSL
                     VLFFAVLINYGLDLFGVRYISNNVKHLRKCKSFLYISILSRLCIFIFCSFFLLVSLMA
                     LTKPDLLYWLLCFFWMLSFVFQSNWYLQGCGDFKLITFYGLLPKLIVYPFVFILVNND
                     NDAWVYLFLCAIANYASNILMMIHIRRKLNVVAFKRTNLLRNVWIFLVKGWYIFLSQA
                     SVTLISYANIFFLPLVLEPKSFVVFSTAERIVKVLSITTTPILNVIFPHISSLIENNK
                     QRVFIMISRISLFSLIVFLLCYTLYVFIGYYLIQLIFPTISQELYSVLLLLLFNVFFV
                     FQNNLFGTQIGLNYGKDKSFTKIIAINGILNIIAMPVFSKLYHLHGTILTSLCIQLSI
                     LICMYMLAKKCGYKFKV"
     gene            7781..8875
                     /gene="wbdG"
     CDS             7781..8875
                     /gene="wbdG"
                     /note="WbdG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27317.1"
                     /translation="MKKIAILLPGPRREPVGGYKVLYQYGNYLASVGWQIDFIYLTNR
                     IVCYNRFSGLVGKIKAKLFSLLKFYKWFDFTSSNINHYVIKSLKKRDIENFNIVICSS
                     VETFIYANSSCRVNHKKLVYFVQDYENWNIPENILNYTLSHKDVDYITISDDLVKKIT
                     KMGGDVSLTLYNGINKNDFFVNIPWNERDRASFLFLYHPSERKGCDLLLRAIQKIHEV
                     DSSIKFSCFSAYTKPENFPSFIHYVYQPTVKQLNNLYNENRYFICSSIYEGFGLTAAE
                     ASFSGAIVVTTRNGGVEQFIKNEVTGFVLKERTLNSFMVAIMKLSQKDEELNLFAIES
                     SKKVRIQLDLDINFKKIDSYFKARYNNKRN"
     gene            8879..10171
                     /gene="wzy"
     CDS             8879..10171
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAL27318.1"
                     /translation="MLAWAPKLLLLFFLLSGLIKAISLYININLNYVFIPLLILASLF
                     YLLINRTIKMKAQGACFIFFFSISAILFLVNNIVVNNVYTQLQLMLAVTIQYIFPIII
                     ICLAGSGKLKLDIFDSLINIITKLTFLSLLIFGLCVMYDSKMVEEFYSQLYNHGLIIN
                     PFQTSEEGITLRFSGIFSSGFILASYCCFAIIYYYFNKSVNRIKFAFLFVGFVIMVVL
                     TYNRNGMLAFAVTSLFIMIHTYLRRYYLTLANLYYVFLIGILFIAPSFILYFGGDIQN
                     SYSSIDYSAFTKLSTLFSRIDAWLQVLNINDVSRLLFGSGLVQGLGEDDMNFYVDNGY
                     LYLLHQSGLIALVLYIATWSYMFISLSKNLKKRRNHNFVSVDEINICLLLILVSFTLA
                     LLNNLFFEPVFLILVLMKCVTVNNKSSSIYEKNEQTYE"
     gene            10164..11219
                     /gene="wbdS"
     CDS             10164..11219
                     /gene="wbdS"
                     /note="WbdS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27319.1"
                     /translation="MSNTAIIGTVGVPASYGGFETLVENLIETEKVNYIVYCSSKNYP
                     KKLSTYKNAKLVYIPLSANGISSVIYDAFSILHAIVFRKVNTLLILGVSGCFILPIIK
                     KISSVKIITNIDGVEWRRDKWGRFAKKFLKWSEKIAVKNSNIVISDNKEIANYVCSEY
                     MVNSQVIAYGGDHSLVFETKRCNDVENSESKYALALCRIEPENNVHVILESFSKVTKK
                     LKFIGNWGSSDYGRSLKKKYSKYNNIEIIDPIYDVEQLNIIRSGCEFYVHGHSAGGTN
                     PSLVEMMFFAKPIICFDCKYNRYTTFEQACYFRDLKDLIIKIESPKERTQGVKLKSLA
                     EKHYTWNRIREQYEQLL"
     gene            11347..>12564
                     /gene="gnd"
     CDS             11347..>12564
                     /gene="gnd"
                     /note="Gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="AAL27320.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRD"
BASE COUNT     3896 a   2034 c   2595 g   4039 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa caggacgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactctgtca ttcagaccaa agaaccgctg
      421 gatcgcgaag gtaaagtcag tcgcatcgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac acactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga ctggtgacag ctacgattgc
      661 ggtaaaaaaa tgggttatat gcaggcgttc gtgaagtatg ggctgcgcaa cctcaaagaa
      721 ggggcgaagt tccgcaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagga taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg
     1141 aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata
     1201 aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcacttgctg atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gcagctgtaa tggcacggat ttttgctcag catcagccgg atgcagtgat gcacttggct
     1381 gctgaaagcc atgttgaccg ttcaattaca ggccctgcgg catttattga aaccaatatt
     1441 gttggtactt atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac
     1501 aagaaaaata gcttccgtct tcatcatatt tctactgacg aagtctatgg tgatttgcct
     1561 catccagatg aagtaaataa tacagaagaa ttacccttat ttactgagac gacagcttac
     1621 gcgccaagca gtccttattc cgcatccaaa gcatccagcg atcatttagt ccgcgcgtgg
     1681 aaacgtacct atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat
     1741 catttcccgg aaaaattgat tccattggtt attctcaatg ctctggaagg taaagcatta
     1801 cctatttatg gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtgggcac
     1921 aacgaaaaga aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta
     1981 ccgaaagaga aatcttatcg tgagcaaatc acttatattg ccgatcgtcc gggacacgat
     2041 cgccgttatg cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa
     2101 acgtttgaga gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt
     2161 gataatgtga aaagtggtgc ctatcaatcg tggattgaag agaactatga gggccgccag
     2221 taatgaatat cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc
     2281 tggcacctct gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt
     2341 ttagtaattc tgaaggtgta gctgaaactg tcaaaaaaat tcgccctgat gttattgtta
     2401 atgcggctgc tcataccgcg gtagataagg ctgagtcaga acccgaattt gcacaattac
     2461 tcaatgcgac aagtgttgaa gcgattgcaa aagcagccaa tgaggtcggt gcatgggtta
     2521 ttcactactc tactgactac gtatttccgg gaaccggtga aataccatgg caggaggcgg
     2581 atgcaaccgc accgctgaac gtttatggtg aaaccaagtt agccggggaa aaagcattac
     2641 aagagcattg tgcgaagcat cttattttcc ggaccagctg ggtctatgca ggtaaaggaa
     2701 ataacttcgc caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gctgttatta
     2761 acgatcagtt tggtgcgcca actggcgcag agttactggc tgattgtacg gcacatgcca
     2821 ttcgtgtggc actgaataaa ccggaagtcg caggcttgta ccatctggta gctagtggta
     2881 ccacaacgtg gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc
     2941 cccttgcact caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc
     3001 gtccgcataa ctctcgcctt gatacagaaa aatttcagca gaactttgcg cttgtcttgc
     3061 ctgactggca gattggcgtg aaacgcatgc tcaacgaatt atttacgact acagctattt
     3121 aatagttttt gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa
     3181 tgaaaacgcg taaaggtatt attttagcgg gcggttctgg tactcgtctt tatcctgtaa
     3241 ctatggctgt cagtaaacag ctattaccga tttatgataa accgatgatc tattatccgc
     3301 tctctacact gatgttagcg ggtattcgcg atattctgat tatcagtacg ccacaggata
     3361 ctcctcgttt tcaacaactg ctgggtgacg ggagccagtg ggggctaaat cttcagtaca
     3421 aagtgcaacc gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg
     3481 gtggtgatga ttgtgctttg gttcttggtg ataatatctt ctacggccac gatctgccga
     3541 agttaatgga cgtagctgtt aacaaagaaa gtggtgcaac agtatttgct tatcacgtaa
     3601 atgatcctga acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg
     3661 aagaaaaacc gttacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgaca
     3721 acgacgttgt cgaaatggcg aaaaacctta agccttcttc ccgtggtgaa ctagaaatta
     3781 ccgatattaa ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggccgtg
     3841 gttatgcatg gctggacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg
     3901 ccaccattga agagcgccag ggactaaagg tttcctgccc agaagaaatt gcttaccgta
     3961 aaggatttat tgatgctgag caggtgaaag tattagctga accgctgaaa aaaaatgctt
     4021 atggtcagta tctgctgaaa atgattaaag gttattaatt aaatgaacgt aattaaaaca
     4081 gaaatttctg atgtgctgat ttttgaacca aaagtttttg gcgatgagcg cggcttcttt
     4141 tttgaaagct ttaaccagaa agtatttgaa gaagctgtag gccgcaaagt tgaatttgtc
     4201 caagataacc attcgaagtc tagtaaaggt gttttacgcg ggctgcatta tcagttagaa
     4261 ccgtatgcgc aagggaaatt ggtacgttgc gctgttggtg aggtttttga cgtagcggtt
     4321 gatattcgta aatcgtcacc aacttttggc aaatgggttg gggtgaattt atctgctgag
     4381 aataagcgcc agttgtggat acctgaagga ttagcacatg ggtttttggt gctgagtgag
     4441 acggcggagt ttttatataa aacgaccaat tattatcatc cagagagtga tagagggatt
     4501 atttgggatg atcctgacat tgacgtaaag tggcctttaa gtatccataa accgatttta
     4561 tctataaaag atgaaaaaca aaagatgttt aaagaaatga tagcgttgga gaaatggaat
     4621 ggagaatact aattattata agttagctat cttaattgta ctttatggaa aagaagtaga
     4681 acagtgtgaa acatttactc atatagatag agttctaaaa aaaaatagga tgtcaaagga
     4741 tgatattatt ctgtgtcttt ggaataatgg cccaagagaa atcgcattta atctgactac
     4801 agattactat accttaaaag tttgtcaaac aataaataat gaatcattaa gttatatata
     4861 taatttattt attaatgagg tggagtcaga aaaatatatt ttactagatc atgattccat
     4921 attgaaatat gattatgttt tggaatgtct taattctaca tctgattgta tcattcctaa
     4981 tataatagta ggaaacaata ttcaaagtcc taaaacatat agcattcaat cgtttaacaa
     5041 gggaaataca gtgcttgcta ttgggagtgg aatttgtata tctaaaaaat tatgtgagaa
     5101 acttcgacaa gtatttggtg atgtttttga ttctaggttt tatttttatg gtgttgattc
     5161 aacttttttt ctacggctga ataaaattgg ggaaagtgag tttataaaaa tcagtgagtg
     5221 cgttgtattg cattcattgt ctagactaga aaatgaagct ataaaagtaa agcaatttag
     5281 aattaaagaa agaagttatg atcaaggatt aacattaaga tattatttct gtaaatttac
     5341 gttttttact gtcgcaaaaa aacttcttgc gaagttgatt tttaataaaa gtgatataag
     5401 tttaatatat tttgcaaaag catattttag cggtaagcat tataagagcg gtaaatcatg
     5461 aagtttatta ttattggcta tagagatgcg tatttttgga atgaatatgg gacatctgtt
     5521 agagatttac aaattgcaga aattctatct agaaagtatc atgttacatt tataaataga
     5581 cctgtgtcga tatatgaaag attaaaaaat aagaagaaag caaaaagtga gtataaagca
     5641 tatagtaata ggataacttt tattgaccat acaagttatg atttaatcgg accgttattt
     5701 ggccgcatat ggacgaaata ttgttacaag aagatattaa atagaatatc atcgttattt
     5761 acagatgatt caatcatcat tgattttacc cctatagccg aattagaatt caagaacaat
     5821 aaacgaataa catattggta tgatttaata gataattttt caattcataa tagattttct
     5881 gaaaaagaaa aaaaagctgt tcgagataaa tatgcttatg tagacgaaaa tgctgatata
     5941 attacagctg tttccgacaa agcgataaaa aattttctaa atcctaataa gtatgtaatg
     6001 ccaaatggtg tttatcttga aaaccaaaat catgaatgtt ttatagatgg aactccaaat
     6061 aaggtatatc aattaggatt tgttggcttc gttactgata aattagatat tgatttttta
     6121 aataaattat caaaaaaatt cagtatcatc atttggggaa aattttttga tgattctata
     6181 cgtgataagc tgaatgaaaa tataaaaata gcgggggcat ttaaatactc tgatcttcct
     6241 tcaataatga aatcttttaa agtcggatta ttgccatatc tgccagaaaa atcgcatgat
     6301 gaatcaccat tgaaaatgta tgagtacttt aaatatggca ttccatgttt atcatctatg
     6361 aattttgaaa ttaagagtcg atggtttgtt aattatctag aatactcaaa aagagatttg
     6421 ttatatgaga aaataaatga attaattgtc tcaaataatt cggaaagcat taaagccagc
     6481 atacaaaaag aatggttatt tgaaataaaa gttaataaaa ttatcgaaga tattattcga
     6541 aggaaagaat gtggtaaaaa aaatagttaa tcaatttatt taccagtttg ggaattatat
     6601 tctacccttt cttataattc ctttcttaat caataaactc tctattgcgg tctataaaga
     6661 ttttgttgtt attcaatcat tagtattatt ttttgctgtt ttaattaatt atgggctgga
     6721 tctgtttggt gttagatata ttagtaataa tgtaaagcat ctaagaaaat gtaagagttt
     6781 tctttatatt tccatattgt cacggctctg tatttttata ttttgctcat tctttttgct
     6841 tgtgtcgttg atggcattga ctaaacccga cctcctttat tggttgctat gttttttttg
     6901 gatgctaagc tttgtatttc aatcaaattg gtatttgcaa ggatgtggtg attttaagtt
     6961 aattacgttc tatggcttac ttcctaagct tattgtttat cccttcgttt ttattttggt
     7021 caataatgat aatgatgctt gggtttatct atttttatgt gcaatagcaa attatgcatc
     7081 aaacatacta atgatgatac atattaggag gaagttgaat gtagtagctt ttaaacgtac
     7141 taatttgctc agaaatgtat ggatattttt agttaaaggg tggtatatat tcctatctca
     7201 agctagtgtg acattgattt cttatgccaa tattttcttt ctacctttgg tgttggaacc
     7261 taaaagtttc gttgtctttt caactgctga aagaatcgtg aaggtgttat cgattacaac
     7321 aacgcctatt ctaaacgtta tttttccaca tatttcatca ttaattgaaa ataataaaca
     7381 aagagtattt ataatgattt ctcgaatatc attatttagt ttaattgttt ttctgttatg
     7441 ttacacgttg tatgttttta taggctatta tctcatacag cttatttttc caacaatatc
     7501 acaagaatta tactctgtat tattattgtt gttatttaat gtattcttcg tattccaaaa
     7561 caatctcttt ggtacacaaa ttggattgaa ttatgggaaa gacaaaagct tcactaaaat
     7621 tattgcaatc aatggtattt taaatattat agccatgcct gtatttagta aactgtatca
     7681 cctgcatggc acaatattaa catccttatg catacaatta tcaattttga tatgtatgta
     7741 tatgttggct aaaaaatgtg gttataaatt taaggtctaa atgaaaaaaa tagctatttt
     7801 actaccagga ccaagaaggg agcctgttgg tggatataaa gtcttgtatc aatatggaaa
     7861 ctatttggca tctgttggat ggcagattga ttttatttat ttgacgaacc gtattgtttg
     7921 ttataatcga ttttctggcc ttgtgggtaa aataaaagca aaattatttt cattattaaa
     7981 gttctacaaa tggtttgatt ttacatcctc taatataaat cattatgtga ttaaatctct
     8041 aaaaaaaaga gatatcgaaa attttaatat tgtcatttgc tcctctgttg agacgtttat
     8101 atacgccaat tcgtcatgta gagttaacca taaaaaatta gtatattttg tacaggatta
     8161 tgaaaactgg aatattcctg agaatatttt aaactataca ctttcacata aagatgttga
     8221 ttatataact atttctgatg acttggtgaa aaagattaca aaaatgggag gggatgtttc
     8281 tctcaccttg tacaatggaa taaacaaaaa tgattttttt gttaacattc catggaatga
     8341 aagggataga gcttcatttt tatttctata tcatcctagt gaaagaaagg gttgtgatct
     8401 cttattaaga gcaatacaaa aaattcacga agttgatagc tcaattaaat tcagttgctt
     8461 tagtgcttat acaaaaccag aaaatttccc ttcgtttatt cattacgtat atcaacccac
     8521 agttaaacag ctaaataatt tatataatga gaatagatac ttcatttgca gtagtatata
     8581 tgaaggattt ggtttgactg cagcagaggc atcttttagt ggggcaatag tagttacaac
     8641 gagaaatggt ggcgtagaac aatttatcaa aaatgaagtg actggctttg ttctcaagga
     8701 aagaacgcta aattccttca tggttgctat tatgaagctt tcacaaaagg atgaagaact
     8761 aaatcttttt gctattgaaa gtagtaaaaa agtcagaata caactagatc tcgatataaa
     8821 tttcaagaaa attgatagtt actttaaggc tagatataat aacaagagga attaatttat
     8881 gctagcttgg gcaccaaagt tgttattgct gtttttttta ttgtctggat taattaaggc
     8941 tatatcattg tatataaata ttaatcttaa ttatgtgttt attcctctat taatattagc
     9001 ctctctgttt tatctgttaa ttaacagaac aataaaaatg aaagctcaag gggcatgttt
     9061 tatatttttc ttttccatat ctgcaatttt gtttcttgta aataacatcg ttgttaataa
     9121 tgtttataca caattacaat taatgcttgc agtaacgata caatatattt ttccaattat
     9181 aatcatttgt ttagccggtt cgggaaagtt gaaattggat atttttgact ctttgattaa
     9241 tataataaca aagcttacct ttttatcact tttaatattt gggttatgtg tgatgtatga
     9301 ctctaaaatg gttgaagagt tttattcgca attatacaat catggtttaa taattaatcc
     9361 atttcaaaca agtgaagaag gtattactct tcgttttagt gggatatttt catctgggtt
     9421 tatcttagct agttattgtt gctttgcaat aatttattat tattttaata aatctgtcaa
     9481 tcgtataaag tttgcttttc tatttgttgg ttttgtcata atggttgttt tgacatataa
     9541 tagaaacggg atgttggcat ttgcagttac atctttattt ataatgattc atacttacct
     9601 tcgtcgatac tatctaactc ttgcgaattt atattatgtt tttttgattg gaatattgtt
     9661 catagcacca agttttatat tatactttgg tggtgacatt cagaattcat attcttctat
     9721 agattattca gcttttacaa aattgagtac attattctct cgtatagatg cctggcttca
     9781 ggttttgaat attaatgacg tctctcgttt attatttggt tccggtttag tgcaggggtt
     9841 aggggaagat gatatgaatt tttacgtcga taatggttat ttatatcttt tgcatcagag
     9901 tggtcttata gctcttgttt tatatatcgc tacttggagt tacatgttta tttctctctc
     9961 taaaaactta aagaaaagac gaaaccataa ttttgtttca gtagatgaaa taaatatatg
    10021 cttacttctt attcttgtaa gtttcacctt ggcactcctt aataatctat tttttgaacc
    10081 cgtatttcta attcttgtat taatgaaatg tgtaactgtg aataataaat caagtagtat
    10141 ttatgaaaaa aatgaacaaa catatgagta ataccgccat tattggtact gtaggtgttc
    10201 ctgcttcgta tggagggttt gaaacacttg ttgaaaattt aattgaaact gaaaaggtta
    10261 attatattgt ttattgctca tcaaaaaatt accccaaaaa actaagcaca tataaaaatg
    10321 cgaaattggt ttatattcct cttagcgcta atggaatatc aagtgttatt tatgacgcat
    10381 ttagcatatt gcatgcgatt gtattccgaa aagtaaatac actccttatt ttaggagtct
    10441 ctggttgctt tattcttcct ataataaaaa aaatatcatc tgttaaaata attacaaaca
    10501 ttgatggagt agaatggcgt cgagataaat ggggtaggtt tgctaaaaaa tttttaaaat
    10561 ggtctgaaaa aatagctgtg aaaaattcta atattgtgat ttctgataat aaggaaatcg
    10621 caaactatgt gtgctcagaa tatatggtaa attctcaagt aattgcctac ggcggagatc
    10681 attcactagt ttttgaaaca aaaagatgta acgatgtgga gaattccgaa agtaaatatg
    10741 ctcttgctct ctgtcgtatt gagccagaaa acaatgttca tgttatatta gaatcttttt
    10801 ctaaagtcac caaaaaattg aagtttattg gtaattgggg aagtagcgac tatgggcgta
    10861 gtcttaagaa aaaatattca aagtacaata atatagaaat tattgatcca atttatgatg
    10921 ttgaacaatt aaatattatt agaagcggat gtgagttcta tgtgcatgga cattctgctg
    10981 gaggtactaa tccatcacta gttgaaatga tgttttttgc aaagccgatt atttgttttg
    11041 attgtaagta taatcgttat acaacatttg aacaagcgtg ttatttcaga gacctgaagg
    11101 atcttataat taaaatagaa tcacccaaag agagaacaca aggtgttaag ttgaagtcgc
    11161 tggctgaaaa acattacaca tggaatagaa ttcgtgaaca gtatgagcag cttttgtaaa
    11221 aatggagtaa gaatattaag aacatataga tatttcaggt aggtaaagtt gaggttataa
    11281 ggtactcttc aaacttgatt caagccgcgc aaacccgtgg tgaacacccc ctgacaggag
    11341 taaacaatgt caaagcaaca gatcggagtc gtcggtatgg cagtgatggg gcgcaacctt
    11401 gcgctcaata tcgaaagccg tggttatacc gtctctattt tcaaccgttc ccgtgaaaag
    11461 acggaagaag tgattgccga aaatccaggc aaaaaactgg ttccttacta tacggtgaaa
    11521 gagtttgttg aatctctgga aacgcctcgt cgcatcctgt taatggtgaa agcaggtgca
    11581 ggcacggatg ctgctattga ttccctcaag ccatacctcg ataaaggtga catcatcatt
    11641 gatggtggta acaccttctt ccaggacacc attcgtcgta atcgtgagct ttctgctgaa
    11701 ggctttaact tcattggtac cggtgtttcc ggtggtgaag agggcgcgct gaaagggcct
    11761 tccattatgc ctggtggcca gaaagaagcc tatgaactgg ttgcgccgat cctgactaaa
    11821 atcgccgcag tggctgaaga cggtgagcca tgtgttacct atatcggtgc cgatggtgca
    11881 ggccattatg tgaagatggt tcacaacggt attgaatacg gcgatatgca gctgattgct
    11941 gaagcctatt ctctgcttaa aggtggcctg aacctcacca acgaagaact ggcgcagacc
    12001 tttaccgagt ggaataacgg tgaactgagc agctacctga tcgacatcac caaagatatc
    12061 ttcaccaaaa aagatgaaga cggtaactac ctggttgatg tgatcctgga tgaagcggct
    12121 aacaaaggta cgggtaaatg gaccagccag agtgcgctgg atctcggcga accgctgtcg
    12181 ctgattaccg agtctgtgtt tgcacgttat atctcttctc tgaaagatca gcgtgttgcc
    12241 gcatctaaag ttctctctgg cccgcaagcg cagccagctg gcgacaaggc tgagttcatc
    12301 gaaaaagttc gtcgtgctct gtatctgggc aaaatcgttt cttacgccca gggcttctct
    12361 cagctgcgtg ctgcgtctga agagtacaac tgggatctga actacggcga aatcgcgaag
    12421 attttccgtg ctggctgcat catccgtgcg cagttcctgc aaaaaatcac tgatgcatat
    12481 gccgaaaatc cgcagatcgc taacctgctg ctggctccgt actttaaaca aattgccgat
    12541 gattaccagc aggcgctgcg tgat
//
