LOCUS       AF402313               15223 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Shigella boydii serotype 5 O-antigen gene cluster, partial
            sequence.
ACCESSION   AF402313
VERSION     AF402313.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Wang,L., Qu,W. and Reeves,P.R.
  TITLE     Sequence analysis of four Shigella boydii O-antigen loci:
            implication for Escherichia coli and Shigella relationships
  JOURNAL   Infect. Immun. 69 (11), 6923-6930 (2001)
   PUBMED   11598067
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   Wang,L., Qu,W. and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2001) Department of Microbiology, The University
            of Sydney, Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /serotype="5"
                     /db_xref="taxon:621"
     gene            <1..765
                     /gene="galF"
     CDS             <1..765
                     /gene="galF"
                     /note="GalF"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate uridylyltransferase"
                     /protein_id="AAL27321.1"
                     /translation="IVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLA
                     EVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRY
                     NLAAMIARLNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQ
                     TLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSY
                     DCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AAL27322.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKQQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2223..3122
                     /gene="rmlD"
     CDS             2223..3122
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AAL27323.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKTANEVGAWVIHY
                     STDYVFPGTGEIPWQETDATAPLNIYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEAARNAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3180..4055
                     /gene="rmlA"
     CDS             3180..4055
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AAL27324.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQIKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            4063..4599
                     /gene="rmlC"
     CDS             4063..4599
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAL27325.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSTTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFITLSNIAEFIYKTTQFYNPSFERVIAWDDPVLNINWNIK
                     QLPFVSKKDMKGLPFRNI"
     gene            4614..5375
                     /gene="wbdT"
     CDS             4614..5375
                     /gene="wbdT"
                     /note="WbdT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27326.1"
                     /translation="MGNKVSLLVNCLNEVNNLKNNIIPLAHHFDEVVIVDMQSNDGTA
                     ELIKKLDNSRFIEIEKMGYVEPARKIGINSCKYDYVFILDADEILSEKIILEINRFRK
                     GEKTEFIGLVDIKGLMIPRYNSMFSQDITWGKFAPENDKQLRFFKKGTVEISSKIHLG
                     IKFKCNMEVVTLPFSSGYYIYHFHSATALEFIGRVVRYAEYENTKQPGKNSFNVTNAI
                     KAFVKEYILSGGYKHGKTGFALAYILSLREFLKGK"
     gene            5379..6470
                     /gene="wzy"
     CDS             5379..6470
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAL27327.1"
                     /translation="MRVFLYVLILYLSVWITNEYSILSFPIEFNFLYLLPQILLLSFV
                     YAFITRAKKKSHQVDILEWVIPITKLSIVLSLIVLIITLPANGRDAKLEIISNNIVLY
                     YLNIFNSSMLIVILSSMFQPCRSIVNKKVFFYIIFAIIAFSLTNLSRSMAFYFIIAIC
                     MSGLKFDIEKKKIISWGMVLIVVMILMPIFQGRTDNVEGALVRSILNIVFYVSYSFGL
                     GEYLIKETHLSGISWGYFGYVLSKILNEPLTSNIFFDNKYLYEFIQLGKSDIYGLLNA
                     NVMYPLWATVYADFGLFSFIPYLFTFFIIVTSYFMRAYILFSWLFFRFAILGFLVSPV
                     LFRDIIFEIVVVLAFEIFIRKNKRICADE"
     gene            6463..7263
                     /gene="wbdU"
     CDS             6463..7263
                     /gene="wbdU"
                     /note="WbdU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27328.1"
                     /translation="MNNFVVIYVCYGDNFNYEIINAKIRRFLAPLDIKAIVIANTKEE
                     YIYFYDENGRAIKKYSGLNSEMEFSGYFYGVAEYFERRNIKNMRQSYIFMNDTLFSHG
                     KLRKIERLGLTEWKLRSLFFKIKNKEIHGFWHKSIYLRETELKKGYFNSKFFILHNWN
                     FTEIKSILNLNGVAVTINDASHYENNIFMSDKYSRFLQRWLHESDGWYKAQGLNSENK
                     KKFVKKATSIVHEHYITKFIEENRIKKHCLINNSRLAKFVMKFIRLEY"
     gene            7263..8411
                     /gene="wzx"
     CDS             7263..8411
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAL27329.1"
                     /translation="MRAGAIVFLRNLLIAVSGFILVSKGVKNLSEADTAFLLLVMLVI
                     NAQNALYEGLFLVHTMSFKDKCARTILKRQNKIQVLIPVLAIIFVLVYSDIVLDISIG
                     YGYVALLYFCFYGNILSISVVNVFCSIRGFTWCFILDIVFTTAFIILSVTYSSRIILI
                     FALLLRVGGSAFAGLIFQVRYKKSNDKKLLSLPLYSMGYFSGTLLSLFRDTLSPLLIG
                     WFVGPSILIAIRIFNTCYSAPGLFASALSKIIVRYARKKQSAKKVFITYLIGLYVLSI
                     GYLLIWYIGGERLYLLLFGKTQYFPQTIFLYSISLFCLFWPLGQTAITGMIFTGSSRL
                     FFIVSIIWTFVSAICIFILFIYGIEFYMLAIAFSQILNILIVYLVRKK"
     gene            8408..9010
                     /gene="wbdV"
     CDS             8408..9010
                     /gene="wbdV"
                     /note="WbdV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27330.1"
                     /translation="MKLLFNFLYRNLKRCIFRLLIRHYFPCDLSIISSNCIGTRLYQS
                     ADKKYLSPTINLWMPPADFLKFSCNLEFYIQQELLITNNKQFQYPCGRLGDITLHFQH
                     YKNFEVAKKKWDERKDRINWDKILFIFTDRDGATIDSLVKMADVSKNVFVFCSSKKRN
                     SIKRHENIIFIRSKENSIGDLYTNYDELLFKFPFIRFSKI"
     gene            9007..10089
                     /gene="wbdW"
     CDS             9007..10089
                     /gene="wbdW"
                     /note="WbdW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27331.1"
                     /translation="MNKKYFVTDLYAHASEHSLFSKAFIQYFKNKDTIFLLNSIHQKF
                     VDDKSISRTFPFKDSNVKNKWLRLFNREIIKLFTLIAYIPYIVITRRKLCILGASNLQ
                     FFILSFLFFLKPKIIVHGQAESLMTNKNDNSLAARLFINGFQRLAKHKAKLLFLSYHI
                     KNNIKEKGSFYFIKHPLPNSGILGRELSDKKDSKLRVAMVGLLRNDKKNSNLIYSLNV
                     NDNVELWAIGRAHHDFIINHHSNIKFKIWDSVYTDEEFEEAIKDIDAFLYLFNNEQYK
                     MTASATALDAVIYQKIVFTLENDAVQSLLENYKNVYVANSIDELSNLINEFKWENKQA
                     ISKEIINDYILCDNNKDIVTIEEWLK"
     gene            10086..10847
                     /gene="wbdX"
     CDS             10086..10847
                     /gene="wbdX"
                     /note="WbdX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL27332.1"
                     /translation="MNEKPLVSIVTPTFNSMKHIEEAYHSLLSQTLDNWEWLITDDCS
                     TDSTYEYLLDLSKHDTRVKPEKLSKNSGAAVARNCSLDRVSGQYIAFLDSDDIWEREK
                     LRMQVIYMEQNNINFSFTMYKCQDENGVLNGKIIDSSHSQSSFSYQDMLKKKATLGCS
                     TVMLRRCLIDEHRMPLIRTGQDYAFWLMLLKENDAFLLKKCLTRYRVTPGSLSRNKFK
                     KARRQWQIYRHYEKINVFRAFYFFCHYGFHAIFRK"
     gene            10828..12255
                     /gene="manC"
     CDS             10828..12255
                     /gene="manC"
                     /note="ManC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AAL27333.1"
                     /translation="MLYFVNRYNMIKPIILAGGNGTRLWPLSRSQFPKQFLPLLDNKY
                     SLFQQTLQRINCLQKLSPLIICNETHRFLVSQQLKDIDCNDANVLLEPIGKNTAPAIA
                     LAAFQYLKDSEDPLLLVLAADHYISDTGIFLESIKKAKKVAEQGNLITFGIVPDYPHT
                     GYGYIERAKEVYDTVYEIRSFKEKPPYDRACEYIETGDYYWNSGMFMFRASVFLNELK
                     KYEPSIYENCKHAIDSATVDSTFIRPEFHSFNKSPNISIDYAVMERTSIGMVISLDAG
                     WSDVGSWSALCDINSKYHSSDNNNNNNVISIDSSNNFILSEDNLVATVGIKDLIIINT
                     KDALLVADKNNDQNVKLVVEQIKKLGRNEHVEHPVNYRKWGRFEQIDEGVGFQVNRIT
                     IDVGKYISKQYHKYRAEHWIIVSGIAEITLDKVTKIYKENESVYVPTNCIHSIKNLGN
                     TILEIIEVQSGTYFGEDDIIRLSTE"
     gene            12265..13686
                     /gene="manB"
     CDS             12265..13686
                     /gene="manB"
                     /note="ManB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AAL27334.1"
                     /translation="MRSSSVVISSNVSFGTSGARGLVRDFTHDVCAAFTHAFLSVIQN
                     NFNFRIVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPCI
                     MVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYYL
                     ERYVSLFNPEILKGKRIGVYEHSSAGRDLYALLFNQLGAEVISLGRSDEFVPIDTEAV
                     SDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKAL
                     AIPVSCNTAIEQSHKFASIQRTKIGSPYVIAAFADLAKQFNSVAGFEANGGFLLASDL
                     QINGKELKSLPTRDAVLPALILLIASRNSTISQLINNLPQRFTWSDRLKNFPSDSSQQ
                     IIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYAE
                     ASDENQARQYVTNVLGNITSLIS"
     gene            13883..15223
                     /gene="gnd"
     CDS             13883..15223
                     /gene="gnd"
                     /note="Gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="AAL27335.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRD"
BASE COUNT     4721 a   2527 c   3163 g   4812 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg acgcgagtgc cgacccgctg
      301 cgttacaacc ttgctgccat gattgcgcgc ttgaacgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactcggtca tccagaccaa agaaccactg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgctattgct
      601 gaactggcaa aaaaacagtc tgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag taggtaatgc tcgtcacatc gtaggcatgc
     1021 acgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg
     1141 aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata
     1201 aataatactc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcacttgctg atgtttctga ctctgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gctgctgcaa tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacttggct
     1381 gctgaaagcc atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt
     1441 gttggtactt atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac
     1501 aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtttatgg cgatctgcct
     1561 catcctgacg aagtaaataa taaagaagaa ttacccttat ttactgagac aacagcttac
     1621 gcaccaagca gcccttattc cgcatcaaaa gcttccagcg atcatttagt ccgcgcgtgg
     1681 aaacgaacct atggtttacc gaccattgtg actaactgtt cgaataacta cggtccttat
     1741 cactttccgg aaaaattgat tccactagta attcttaatg ctctggaagg taaggcattg
     1801 cctatttatg gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcacgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac
     1921 aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta
     1981 ccgaaagaga aatcttatcg tgagcaaatc acttatgttg ccgatcgccc gggacacgat
     2041 cgccgttatg cgattgatgc agagaagatt agccgcgaat tgggctggaa acagcaggaa
     2101 acgtttgaga gtgggattcg taaaacggtg gaatggtacc tgtccaatac aaaatgggtt
     2161 gataatgtga aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag
     2221 taatgaatat tctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc
     2281 tggcaccttt gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt
     2341 ttagtaatcc tgaaggtgta gctgaaaccg taagaagcat tcggcctgat attattgtca
     2401 acgcagccgc tcacaccgca gtagacaaag cagaatcaga accggagttt gcacaattac
     2461 ttaacgcgac aagtgtcgaa gcgattgcga aaacagccaa tgaagtcggc gcctgggtta
     2521 ttcactactc tactgactac gtatttccgg ggaccggtga aataccatgg caggagacgg
     2581 atgcaaccgc accgctaaat atttacggtg aaaccaagtt agctggagaa aaagcattac
     2641 aagagcattg tgcgaagcac cttattttcc gtaccagctg ggtatatgca ggtaaaggaa
     2701 acaattttgc caagacaatg ttacgtctgg caaaagagcg cgaagaatta gctgtcatta
     2761 acgatcagtt tggtgcacca acaggtgctg aactgctggc tgattgtacg gcacatgcaa
     2821 ttcgtatggc actgaataaa ccagaagtcg caggtttgta ccatctggta gccagtggta
     2881 ccacaacctg gcacgattat gctgcgctgg tttttgaagc ggcgcgcaat gcaggcattc
     2941 cccttgcact caacaagctc aacgcagtac caacaactgc ctatcctaca ccagctcgtc
     3001 gtccacataa ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc
     3061 ctgactggca ggttggcgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt
     3121 aatagttttt gcatcttgtt cgtgatgatg gagcaagatg aattaaaagg aatgatgaaa
     3181 tgaaaacgcg taaaggtatt attttagcgg gtggatctgg tacacgtctt tatcctgtga
     3241 ctatggctgt cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc
     3301 tctctacact gatgttggcg ggtattcgcg atattctgat tattagtacg ccacaggata
     3361 ctcctcgttt tcaacaactg ctgggtgacg gtagccagtg ggggctaaat cttcagtaca
     3421 aagtgcaacc gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg
     3481 gtggtgatga ttgtgctttg gttcttggtg ataatatctt ctacggtcat gatctgccga
     3541 agttaatgga tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta
     3601 atgatcctga acgctacggt gtcgttgagt ttgataaaaa cggtacggca atcagcctgg
     3661 aagaaaaacc gctacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata
     3721 acgacgttgt cgaaatggcg aaaaacctta agccttctgc ccgtggtgaa ctggaaatta
     3781 ccgatattaa ccgtatttat atggaacagg ggcgtttatc cgttgccatg atggggcgtg
     3841 gttatgcatg gctggatacg gggacacatc agagtcttat tgaagcaagc aacttcattg
     3901 ccaccattga agagcgccag ggactaaagg tttcctgccc agaagaaatt gcttaccgta
     3961 aagggtttat tgatgcagag cagattaagg tattagccga accgctgaag aaaaatgctt
     4021 atggtcagta tttgctgaaa atgattaaag gttagtaata aaatgaacgt tattaaaaca
     4081 gaaattccag acgtactgat ttttgaaccg aaagtatttg gtgatgaacg tggattcttt
     4141 tttgaaagtt ttaaccaaaa gatttttgag gaagcggtag gtcgcaaagt tgagtttgtt
     4201 caggataacc attctaaatc ttccaaaggg gttttacgtg gtctgcatta tcagttagaa
     4261 ccttatgcac aaggaaaact ggtacgctgt gttgtcggtg aagtttttga tgttgcggtt
     4321 gatattcgta agtcgtcaac tacgtttggc aagtgggttg gtgtgaattt atctgctgag
     4381 aataagcgcc agttgtggat cccagaggga tttgcgcatg gattcataac gttgtcaaat
     4441 atagccgaat ttatttataa aacaacgcaa ttttataacc cgtcatttga aagagtcatc
     4501 gcatgggatg atccagttct taacattaat tggaatataa aacaattacc ttttgtttct
     4561 aaaaaagata tgaaagggtt accattcagg aatatatagt acagggtttc attatgggaa
     4621 ataaagtatc tcttctagtt aattgtctta atgaagttaa caatctgaaa aataatatta
     4681 tacctctagc gcatcatttt gatgaagtag taatcgttga tatgcaaagt aatgatggta
     4741 ccgcagaatt aataaaaaag ttagataatt cgagatttat agaaattgaa aagatgggat
     4801 atgttgagcc ggcacgaaaa ataggtataa actcttgtaa atatgattat gttttcatat
     4861 tggacgcaga cgaaatttta tcagaaaaaa ttattttaga aatcaataga tttcggaaag
     4921 gtgaaaaaac agaatttata ggattagtgg atattaaagg cttaatgatc ccacgttata
     4981 attcaatgtt ttctcaagat attacgtggg gtaagtttgc tccagaaaat gataaacagt
     5041 tgcgcttttt taagaaagga accgtggaaa tatctagtaa gattcattta ggtataaaat
     5101 tcaagtgtaa tatggaagtt gttacactac cattttcttc aggatactat atttatcact
     5161 ttcattcagc aactgcgtta gagtttattg gtagagttgt tcgttatgct gaatatgaaa
     5221 atactaagca acccgggaag aacagtttta atgttacgaa tgcaatcaag gcgtttgtaa
     5281 aagaatatat actaagcgga gggtataaac atggcaaaac tggatttgcg ttggcttata
     5341 ttttatcttt gcgtgagttc ttaaaaggta aataatatat gagagttttt ctatatgtcc
     5401 tcatattata tttatcagta tggataacaa atgaatatag tattttgagt tttccaattg
     5461 aatttaattt tctatatctg ctcccacaaa tactcctttt gtcatttgta tatgccttta
     5521 ttacaagagc gaaaaaaaaa tcacatcaag tagatatact tgaatgggta ataccaatca
     5581 ctaaactaag catagtttta tctttaattg ttttaattat tacattgcct gcaaatggtc
     5641 gtgacgcgaa acttgagata atatcaaata atatagtact atattatttg aatatattta
     5701 acagtagtat gttaattgta atactgtcaa gtatgtttca accatgcaga tcaatagtta
     5761 ataagaaagt gtttttctat atcatatttg ctataattgc tttctcatta acaaatctgt
     5821 cacgcagtat ggctttttat tttataatcg ctatttgcat gtctggatta aaatttgaca
     5881 tagaaaagaa aaaaataatt tcgtggggta tggtattgat tgttgttatg atattaatgc
     5941 caatttttca ggggcgtaca gacaatgttg aaggtgcttt ggtccgtagc atattaaata
     6001 ttgtattcta tgtatcttat tcatttggtc ttggtgagta cttaataaaa gagactcatt
     6061 tatcaggtat tagttgggga tattttggct atgttttgtc taaaatattg aatgaacctc
     6121 tcacatctaa tatatttttc gataataaat atctttatga atttatccag ttgggcaagt
     6181 cagatatata tggtctatta aatgccaatg taatgtaccc gctatgggca actgtatatg
     6241 ctgactttgg tctgtttagc tttattccgt atctttttac tttttttatt attgtgacct
     6301 cttattttat gagagcgtat atattgtttt cttggctgtt ttttagattt gccatacttg
     6361 gttttttagt ttcacctgta ttatttagag atattatatt tgaaattgtc gtggtgttgg
     6421 cttttgaaat atttataaga aaaaataaaa ggatatgtgc cgatgaataa ttttgttgtg
     6481 atatatgttt gctatggcga taattttaat tatgaaataa ttaatgcaaa gattcgaagg
     6541 tttttagcac cattagatat taaggctatc gtaatagcta atactaaaga agagtatata
     6601 tatttttatg atgagaatgg aagagcaatc aaaaaatatt ctggactaaa tagtgaaatg
     6661 gaattttcag gctattttta tggtgttgcg gaatattttg agagaagaaa tataaaaaat
     6721 atgaggcagt cttatatttt tatgaacgat acattattta gccacggaaa attgaggaaa
     6781 attgaacgac ttggccttac agagtggaaa ttacggagtt tattctttaa gattaagaat
     6841 aaagaaattc atggtttttg gcataaatcg atatatctac gtgagactga gttgaaaaaa
     6901 gggtatttta attcaaaatt tttcattctt cataattgga attttactga aattaaaagc
     6961 attttaaact taaatggagt agctgtaacc ataaatgatg cttcacatta tgaaaataat
     7021 atctttatgt ccgataaata ttcccgtttt ctgcagcgct ggttacatga aagtgatggg
     7081 tggtataaag cacaaggatt aaatagtgaa aataaaaaaa agttcgtgaa aaaagcgaca
     7141 tctattgtac atgaacatta tataacaaaa ttcattgagg aaaatagaat aaaaaagcat
     7201 tgtctaatta ataattctag acttgcaaaa tttgttatga aatttattag gttggaatat
     7261 taatgagggc aggtgctata gtatttttac gaaatttgct cattgctgtt tctgggttta
     7321 ttttagtatc aaaaggagta aaaaatttaa gcgaagcaga tactgcattt ttgctattag
     7381 taatgcttgt cataaatgca caaaatgcat tgtatgaagg gctgttttta gttcatacga
     7441 tgtcgttcaa agataagtgt gcacgtacta ttttaaaaag gcaaaataaa atacaagtgc
     7501 taattcctgt gcttgctata atttttgtat tggtctatag cgatatagtt ttggacatct
     7561 ctataggtta tggctatgta gctcttttgt atttttgttt ttatgggaat attctctcaa
     7621 ttagtgttgt taatgtattt tgttcaataa gaggatttac atggtgtttt attcttgaca
     7681 tagtattcac gacagcattt attattcttt ctgttactta ttcaagtaga ataatattga
     7741 tttttgctct gctcctaaga gttggtggaa gcgcttttgc aggtttgata tttcaagtga
     7801 ggtataaaaa aagtaatgat aagaaattgt tatcattacc gctatactct atgggatact
     7861 tttctggcac gttgctttca ttatttagag ataccttatc accattattg attggatggt
     7921 ttgttggccc atcaattctg attgcaatta gaatttttaa tacgtgctat agtgcaccag
     7981 gattgtttgc gagcgcatta agtaaaatta tagtcaggta tgctaggaaa aagcaaagtg
     8041 ccaaaaaagt tttcatcact tatctgatcg gattatatgt tttatcaata ggttatctat
     8101 taatttggta tattggcggg gagcgattgt atctcctttt gtttgggaag acacaatatt
     8161 tcccccaaac gatttttctt tattcaatat ctctcttttg tttgttctgg ccattagggc
     8221 agacggcaat cacaggtatg atttttactg gttcttctcg tttatttttt atcgtttcta
     8281 ttatatggac attcgtatct gcaatatgca tatttatatt atttatatac ggaattgaat
     8341 tttatatgtt agcgattgca ttttctcaga tattaaatat tcttatagta tacttggtga
     8401 gaaaaaaatg aaactattat ttaatttctt gtatagaaac ctaaaaagat gtatttttag
     8461 attgttgatt agacattact ttccctgtga tttaagtatt atatcttcca attgcattgg
     8521 gactcggtta tatcaaagtg cagacaaaaa atacctgtct cctacaatta atttatggat
     8581 gcctcctgca gattttctaa aattttcttg taacctagaa ttttatattc aacaggaact
     8641 tctgattact aacaataaac agtttcagta tccatgcggt cgactaggag atataacgct
     8701 acattttcag cattataaaa attttgaagt tgctaaaaaa aaatgggatg aacggaaaga
     8761 taggataaat tgggataaaa tactttttat ttttacagat agggacggtg ctacaatcga
     8821 tagtttagta aaaatggctg atgtttctaa aaatgtattt gttttctgtt cgtctaaaaa
     8881 gaggaatagc atcaagagac atgaaaatat aatcttcata aggtcaaaag agaatagtat
     8941 tggtgattta tataccaact atgatgagct attatttaaa tttccattta taagattttc
     9001 taaaatatga ataaaaaata ttttgtcaca gatttatatg ctcatgcctc tgagcattct
     9061 ttattctcca aagcatttat acagtatttt aaaaataaag atacgatttt tcttctgaat
     9121 tcaatccatc aaaaatttgt agatgataaa tccatttccc gtacttttcc atttaaagat
     9181 agtaatgtaa aaaacaaatg gctcaggctt ttcaataggg aaataataaa gctttttact
     9241 ctgattgcgt atattcccta tatagttata actagaagaa aattatgcat attgggggct
     9301 agtaatttgc aattttttat attatctttt ttattttttt taaagccaaa gattattgta
     9361 cacggacagg ctgaatcttt gatgacgaat aaaaatgata attcacttgc tgcaagacta
     9421 tttatcaatg gttttcaaag attggcaaag cataaggcaa aattattatt tcttagctat
     9481 catataaaaa acaacataaa ggaaaaagga agtttttatt ttattaagca tccattacca
     9541 aatagtggga tattagggag agagctatca gataaaaaag attctaagct cagagttgca
     9601 atggtgggat tattgagaaa tgataaaaaa aattccaatt taatttattc attaaatgtt
     9661 aatgataatg tagaactatg ggcgataggt cgagctcatc acgacttcat tataaatcat
     9721 cactcaaata ttaagttcaa gatatgggac tctgtatata cggacgaaga attcgaagag
     9781 gcaattaagg atattgatgc atttttatat ttatttaata atgaacagta caagatgaca
     9841 gcatctgcaa ctgctttaga tgctgtaata tatcaaaaaa ttgtttttac tcttgaaaat
     9901 gatgctgttc aaagtttgct agagaattat aaaaatgtgt acgtggctaa tagcattgat
     9961 gagctatcta atttaattaa tgagtttaaa tgggaaaata aacaagctat cagtaaagaa
    10021 attattaatg actacatttt atgcgacaat aataaggata tagttactat tgaggaatgg
    10081 cttaagtgaa cgaaaaacct cttgtttcga ttgtaacacc aacctttaat agcatgaagc
    10141 atattgaaga ggcatatcat tctcttcttt cacaaacgct agataattgg gagtggttaa
    10201 ttacggatga ttgttctacg gattctacct acgaatatct tctcgattta tcaaaacacg
    10261 acaccagagt taaacctgaa aaattatcaa agaatagcgg agctgcagtt gctaggaatt
    10321 gctcattgga tagagtctct ggacaatata ttgcatttct tgattctgat gatatttggg
    10381 aaagagaaaa gttaagaatg caagttatat atatggagca gaataatatt aacttttcat
    10441 ttactatgta taaatgccaa gatgaaaatg gagtgctgaa tggaaaaatc atagattcat
    10501 ctcattctca gagttctttt tcataccaag atatgcttaa aaaaaaagct acactgggtt
    10561 gttctacggt gatgttgaga cgttgcttga tagatgagca taggatgcct ttaataagaa
    10621 cgggtcagga ttatgctttt tggttaatgt tacttaaaga aaatgatgct tttcttttga
    10681 aaaaatgttt aacgcgatac agagtaactc ctgggtcact ttctcgaaat aaattcaaaa
    10741 aagcaagaag acaatggcaa atatacaggc attatgaaaa aattaatgtt tttcgcgctt
    10801 tttatttttt ctgccattat ggctttcatg ctatatttcg taaataggta taatatgatt
    10861 aaaccaatta tccttgcagg tggtaatggt acacgccttt ggcctttgtc gcgttcgcaa
    10921 tttccaaaac aatttttacc tttgcttgat aataaatata gtttatttca gcaaacctta
    10981 caaagaataa attgccttca gaagttatca ccactaatca tatgcaatga aactcatcga
    11041 tttttggttt ctcagcagtt aaaagatata gattgtaatg atgcaaatgt acttttagag
    11101 ccaatcggga aaaatacagc gccagcaatt gctcttgcag cattccagta tctgaaagac
    11161 agtgaagatc ctttattgct cgttttagct gcggatcatt atatttctga tactgggatt
    11221 tttcttgagt ctattaaaaa agcgaagaaa gttgcagaac aaggaaattt aattacattt
    11281 ggtattgttc cagactatcc acacactggt tatggatata tagaaagagc taaagaggta
    11341 tatgatacag tttatgaaat tcgctcattc aaagaaaaac caccttatga tcgagcttgt
    11401 gaatatatag agacaggaga ttattattgg aatagcggga tgttcatgtt tcgtgcatct
    11461 gtttttttga acgaattaaa gaaatatgag cctagtatat atgaaaattg taagcatgct
    11521 attgattctg ccactgtaga ttctacgttt attcgtcctg aatttcattc atttaacaaa
    11581 agccccaata tatcaataga ttatgctgta atggaaagaa cgagtattgg gatggttatt
    11641 tcattagatg ctggttggag cgacgtcggc tcttggtctg cactttgtga tataaactca
    11701 aagtatcatt cttcagataa taacaacaat aataacgtta tatcaattga tagctcgaat
    11761 aattttattc tttcagagga taatcttgta gcaacagttg gtattaagga tcttattatt
    11821 attaacacga aagatgctct tcttgtggcc gataaaaata atgatcaaaa cgtaaaatta
    11881 gttgtggaac aaataaaaaa gttaggtaga aatgaacatg ttgaacatcc tgtcaattat
    11941 aggaagtggg gaaggtttga acaaattgat gaaggagtgg gatttcaagt aaatcgtata
    12001 acaattgatg tgggaaaata tatttcaaaa caatatcata aatatcgcgc tgaacattgg
    12061 ataattgttt ctggtattgc tgagataaca ttagataagg taacgaaaat ttataaggaa
    12121 aatgaatctg tatatgttcc gacaaattgc attcactcca ttaaaaacct tggtaataca
    12181 atattagaga ttattgaagt gcaatcagga acatattttg gtgaagacga tataattaga
    12241 ttaagtacag agtgagaaaa tatcatgcgt tctagtagtg tagtaataag ctctaatgtt
    12301 tcatttggca ctagcggggc acgcggttta gtgcgcgatt ttactcatga tgtttgtgca
    12361 gcgttcaccc acgctttcct ttcagtgata cagaataact ttaacttccg tattgttgcc
    12421 ttagccattg ataaccgccc aagtagttac gaaattgctc aggcatgcgc gtatgctata
    12481 aaacaacatg gattcgaggt tgaatatcat ggtgtaattc cgactcctgc attagctcat
    12541 tattcgatgc agaaaaacat tccctgcatc atggtcacag ggagtcatat cccttttgat
    12601 cgtaatggct taaaattcta cagacctgat ggtgaaatca ctaaagagga tgagctcgca
    12661 attataaata gtgagtatac attttcgcct gtaggtgtat tacctcatct tgaaacaagc
    12721 actcaaggtg cagactacta ccttgagcgt tatgtttctc tttttaatcc cgaaatttta
    12781 aaggggaaaa gaataggggt atatgaacac tctagtgcag gacgcgattt atatgctctt
    12841 ctttttaatc aattgggtgc agaggtcatt tccttgggta gaagtgatga gttcgttccg
    12901 attgatacgg aagcagtaag tgatgaagat cgtatacttg caagagagtg gtctaaaaaa
    12961 tataatcttg atgctatttt ctcaacagat ggcgatggtg atcgtccttt agttgccgat
    13021 gaaaatggtg aatggctaag aggcgatatt ctggggttac ttaccgctat tgaacttaat
    13081 atcaaggcgt tggctattcc agtgagttgt aatacagcaa ttgaacagtc tcataaattt
    13141 gcaagtatac aacgaacgaa aataggctct ccttatgtaa ttgcagcgtt tgcagatctt
    13201 gctaagcaat ttaattcagt tgctggtttt gaagctaatg gcggttttct ccttgcctcc
    13261 gatttacaaa ttaatggcaa ggaattaaaa tcgttaccta cacgagatgc tgtgttacca
    13321 gcattaatac tcttaatagc ttctcgcaat agtactattt ctcaactgat taataatctt
    13381 cctcagcgat tcacttggtc agatagactt aaaaacttcc cttcagattc aagtcaacaa
    13441 attataaaaa atgccatatc gtcacccaat aatttcttta atagtttagg ttatgaatca
    13501 ttatcctgct ccgctattga tgaaacggat ggtgcaagat ttactttaaa taatggtgat
    13561 attatacatc tccgtccttc cggtaatgct ccagaactcc gttgttatgc tgaggccagt
    13621 gatgaaaatc aggctaggca atatgttacg aatgtgctgg gaaatattac ctctttgatt
    13681 tcttgatgtt ataggtttat ctacgcttat atgtgtgcgt aggtttgatt acacgtagat
    13741 gccggtatac agaattgaag aacggtattt attacattaa tgaaattcag cactacacac
    13801 attcgtgcaa cttgagataa catctcaatc atattcaagt cgcgcataca tcgcggtgaa
    13861 caccccctga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt
    13921 gatggggcgc aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa
    13981 ccgttcccgt gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc
    14041 ttactatacg gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat
    14101 ggtgaaagca ggtgcaggca cggatgctgc tattgattct ctcaagccat acctcgataa
    14161 aggcgacatc atcattgatg gtggtaacac cttcttccag gacaccatcc gtcgtaaccg
    14221 tgagctttct gcagaaggct ttaacttcat cggtaccggt gtctccggcg gtgaagaagg
    14281 cgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg aactggttgc
    14341 tccgatcctg accaaaatcg ccgcagtagc tgaagacggt gagccatgcg ttacctatat
    14401 tggtgccgat ggtgcaggtc attatgtgaa gatggttcac aacggtattg aatacggtga
    14461 tatgcagctg attgccgaag cctattctct gctaaaaggt ggcctgaacc ttaccaacga
    14521 agaactggca cagaccttta ccgaatggaa taacggtgaa ctgagcagct acctgatcga
    14581 catcaccaaa gatatcttca ccaaaaaaga tgaagacggt aactacttgg ttgatgtcat
    14641 tctggatgaa gcagcaaaca aaggtacggg taaatggacc agccagagtg cgctggatct
    14701 cggcgaaccg ctgtcgctga ttaccgagtc tgtgtttgca cgttatatct cttctctgaa
    14761 agatcagcgc gttgctgcgt ctaaagttct cactggcccg aaagcgcagc cagcaggcga
    14821 taaggctgag ttcattgaga aagttcgtcg tgctctgtat ctgggcaaaa tcgtttctta
    14881 cgctcagggc ttctctcagc tgcgtgcggc gtctgaagag tacaactggg atctgaacta
    14941 cggcgaaatc gcgaagattt tccgtgctgg ttgcatcatc cgtgcgcagt tcctgcagaa
    15001 aatcaccgat gcatatgccg aaaatccgca gatcgctaac ctgctgctgg ccccgtactt
    15061 caagcaaatc gccgatgact accagcaggc gctgcgcgat gtcgttgctt atgcagtaca
    15121 gaacggtatc ccggttccga ccttcgccgc tgcggttgcc tattatgaca gctaccgtgc
    15181 cgctgttctg cctgcgaact taatccaggc acagcgtgac taa
//
