LOCUS       EU296411               15796 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella boydii type 7 O-antigen gene cluster, complete sequence.
ACCESSION   EU296411
VERSION     EU296411.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 15796)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15796
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="type: 7"
     gene            1088..2092
                     /gene="psb1"
     CDS             1088..2092
                     /gene="psb1"
                     /note="dehydratase/epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb1"
                     /protein_id="ACD37064.1"
                     /translation="MSYFDNKTILITGGTGSFGNKFVRMTLDKYNPKKIIIYSRDEMK
                     QWEMAKHFKDESRIRFFIGDVRDKDRLYRALDGVDFVVHAAATKIVPTAEYNPFECVK
                     TNINGAMNVIDACIDKGIERVVALSTDKASSPANLYGATKLASDKLFVAGNSYSGATK
                     TRFAVVRYGNVMGSRGSVIPFFLSIKEKGELPITDDRMTRFMITLEQGVELVWHAFED
                     MVGGEIYVKKIPSMKVTDIATAVAPNATQKMVGIRPGEKLHEQMISAEDAYYTYEYPE
                     HFKILPAIHNWCNSPERIKDGKKVPEGFVYESDSNAEWMSIEELRQWIEDNREKVGNI
                     "
     misc_feature    1088..14344
                     /note="O-antigen gene cluster"
     gene            2092..3243
                     /gene="psb2"
     CDS             2092..3243
                     /gene="psb2"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb2"
                     /protein_id="ACD37065.1"
                     /translation="MKFIPYGRQDVSDEDIHAVVDVLKSDFLTQGPCVPQFEKAIADY
                     VNAKYAVAVNSATSALHIACLALGMKKGDWLWTSPNTFVASANCALYCGANVSFVDID
                     ARTYNMSVSALEAKLMSARNNGTLPKVVVPVAFAGQSCEMEEIYKLSKEYGFSIIEDA
                     SHAIGGNYQGKKIGNSRFADITILSFHPVKIITTAEGGMALTNNDDLAEKMQLFRSHG
                     ITRDSNHMTKVSEGGWYYQQVDLGLNYRMTELQAALGVSQLKSIDDFVTRRHELVERY
                     KKALEDIPISLPYQAESGHSAFHLYPVTVKNSAQRKTLFDYLRNKNIGVNVHYIPVHT
                     QPYYEKLGHKIGDYPVAEDYYSRTISIPMYSALTNEDQDYVIQCIREFF"
     gene            3244..3948
                     /gene="psb3"
     CDS             3244..3948
                     /gene="psb3"
                     /note="cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb3"
                     /protein_id="ACD37066.1"
                     /translation="MNVAIIPARGGSKRIPRKNIKMFCGKPMIAWSINAARKSGVFDR
                     IIVSTDDMEIAAVARKFGAEVPFMRPEELSNDFTGTIPVIRHAAEWLISQGNKIKYIC
                     CIYATAPFIRDEDLKLGLNLIKAKGGDYCFSVTTYSYPIQRALKINENNEITMFDPTQ
                     FEMRSQDLVDAWHDAGQFYWGNIDAWIKEKPVFKSQAIPLPLPRERVQDIDTPEDWHF
                     AEVLYRINTIKNEDCI"
     gene            3932..5032
                     /gene="psb4"
     CDS             3932..5032
                     /gene="psb4"
                     /note="nucleotidase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb4"
                     /protein_id="ACD37067.1"
                     /translation="MKIVFRVDASLSIGSGHVFRCLNLAGALRKNGFECIFLTKEHAG
                     NLIQYIKTYLFQVYVISKEDNVDDKYIENEKEWLGGSQKEDAEKTCEIIYRNNFAPGI
                     FIIDHYSLDSEWESLIKWKFPSTKIVVIDDLCNRKHYCDILIDSTFERTSEEYRRLVP
                     EYCTILAGTDYALLKDEFLQLRKEAILKRTSILSPQKILITMGGVDIHNVTGKVLKEL
                     NNRADLHYKKITVVLGVNCPHKKNIENIAQSIKYDVDIKVNTNNMPQLMLEHDLSIGA
                     LGSTTWERAVLGLPTVNIAIADNQLVIVEKLRRNGFIVFDSINFTGVELARSLIKVTD
                     FYDEMVSRSLSICDGRGLQRTLKFIISLATNK"
     gene            5039..5638
                     /gene="psb5"
     CDS             5039..5638
                     /gene="psb5"
                     /note="N-(3-hydroxybutanoyl) transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb5"
                     /protein_id="ACD37068.1"
                     /translation="MLMTGKNIKKLNLLNIVHSSEDIQEKVRCIRNEPQIRKWMYTDH
                     LISKDEHKNWIERLRNESRNFVYVVMNDYDEPIGVVSVNSIDLKNKKGDWAYYLTANT
                     RTGIGAVLEFFVIDYVFNYLHLEKLNCEVIEHNDTVVRLHEKFGFKQEGFRESNIIKD
                     DKRFGVYYFGLTKEKWMLTRECLKNKYYKLFSQYAVDFH"
     gene            5662..6717
                     /gene="psb6"
     CDS             5662..6717
                     /gene="psb6"
                     /note="condensase"
                     /codon_start=1
                     /transl_table=11
                     /product="Psb6"
                     /protein_id="ACD37069.1"
                     /translation="MKAITIAGRKIGKDFPPYIIAELSANHNGDINRAFKIMEEAKAA
                     GADAVKLQTYRADTITLDCDSEEFQIHGGLWGGQTLYSLYQSAQMPWEWHKPLFDKAK
                     ELGITIFSSPFDFTAVDLLEELGAPAYKIASFEAIDLPLIRYVAQTGKPMIISTGMAD
                     EQEIQEAIHVAKEGGCKELVVLHCVSGYPAPPEDYNLATIPDMAKKFNVITGLSDHTI
                     DNTTAIASVALGASVIEKHVTLDRNGGGPDDSFSLEPSELTVLCRDAKIAWQALGRIN
                     YTRKPSEMGNVQFRRSLYFVKDMKAGDIIDETCVRSVRPGYGLPPKYFDDLIGKRVIS
                     DVMANTRTSLELIADLK"
     gene            6714..7928
                     /gene="wzx"
     CDS             6714..7928
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD37070.1"
                     /translation="MKVIKDSVLYVIGELISKSVPFLLLPYLTRRLGIDGFGEMSFYQ
                     TILALLVIVISLGQDGAVARYFYFYGARSINLIVTVGALYSLVTTSLLFLSFSFFKST
                     ILSIIVLTAGFQSLFNTQLSLRQCQKESVQYIVMQILVALTSGGITFCLLEIYSHDLV
                     EKRFIALLLSNIVCVFIAYLIYMRRRKYNHAFLFRRYYSALLYLIGFGGPLILHQISG
                     FLKGQLDRVLIYSRFSASDLGGYSAAFQIASVLLILIMALNKALVPYYYSELKKKHIV
                     FDDIKKTCVFLFLLIPLPSIISLIIPADFWCFILGNEFVDVKYYIAIFLLGIGFTIPY
                     LLLVNYLFYYGKNKSITICSVISTSIYCIVLYFLSCLGISYVPYSMLIANMLIVPLLY
                     MAAKKTSMLERQ"
     gene            7936..8877
                     /gene="wfdL"
     CDS             7936..8877
                     /gene="wfdL"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37071.1"
                     /translation="MPSLFICVTPLQMLIAEKIIDKTRPVNIEIIVLAYQKNDKYMHY
                     IKRLEKKCTNFTVLAVTPKNKFVTVIAFAKLHTILNKNMSKTYSEVYLSSIDNKYVQL
                     IVSKLNYARLYTFDDGTANIIKSSAYYQEEKKTLKTNILRWIFGINKGLQEIKSEICK
                     HYTIYPSVSNIVSNTELIEMFTQCKKRKQDKKVVRVFIGQPFDELGIPLSLIEEFFFK
                     YKMDYYYPHPREKIINNKFTYIHSHLIFEEYIIEGLQHQDIVYKIYGAVCTSILNLAS
                     SKNDIEICSIYTDELRTKYSDYYALAEKMNITLLKLT"
     gene            8889..10184
                     /gene="wzy"
     CDS             8889..10184
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACD37072.1"
                     /translation="MTNITFRTLRIPGYLLSLFVLLIGFITYFIFNETLAANLLAVGA
                     IGIIILDSLHDKRIFYLTLFPVYVLVGQLISLLLVEHGWILIELGGIKSYPIGSILFM
                     ALTIVLFHFIIFLTVKISIFKFDRTCIDKLSGNSFIYYLPIFYLCLVYIPVFIYGSAL
                     SVTNGNRVVYNQIISPVFLYLFQIKQFILPVAGLYLLKNKKIFTIYLFAILLWNILIG
                     EKATGIWQSLYPMLLPYVLINYDKIKTKNILIVLGFCIVFITSSIVINYIFIEKSGAT
                     FIFDRISMQGQLWWYYFNEHVLLAKTPHELSEEFSSEYSGLLNLMYHSMPAHLFNSYI
                     ERGVVLTSGFPAIFLFYFGQYWLLPTLLSPFLFGLVVYFFSRSLYSGSILSLLISSKL
                     FFAFTVFFARGDIATFLDYKLLIYLLIIIILQYLPRVKV"
     gene            10186..11148
                     /gene="wfdM"
     CDS             10186..11148
                     /gene="wfdM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37073.1"
                     /translation="MAYTSHFIYKVANKISIFIRKIINLFRLFLYGDVVSYDFEMEEN
                     RRIIVSGSLTKRDNIPKIIWIYWHDEQIPLLIEACIKRLKDLNPNYQLNVLNKFTIHE
                     YLPESLMWRQDLSVQIRSDLIRLGLLYKFGGVWIDASVMFFEDLSWLERLSAENKYDL
                     IGFYRGRLSIDCYNPIVESWMLGAPKYNKFIKRWYDNFYPVSYLGLDEYFNTIKKRSD
                     YKIIKQNINIPKYLSVYLAQQISSREMRDYCAYLRKVEDSAYLYQESYPQRDCVLSEV
                     WCKRRKPQKIPPLIKLTSQNRNTILACSKINPRSIVGEFLDNKN"
     gene            11160..12272
                     /gene="wfdN"
     CDS             11160..12272
                     /gene="wfdN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37074.1"
                     /translation="MRDKIVFVVADITATGGIERVITYLASYLCNNGYSVEILSIHRS
                     NRNLPYPVHADVHISFVDKTIMKARKPGSVSKLLSHFKSSFHLNIKLFKRRKCIIVAN
                     SFPVALFSCFSALFSSKMLVVEHVHYHYYSKCLVNVRRFIYKFFYGVVALTERDSELY
                     INDKLNSLTIPNALSSFPEKIANPSVEKKKIIAAGRLEHQKGFDILIKSFAEIDYSVR
                     KEWIMDIYGDGNERAALQKLIHDLNIEDCVNLLGNSRDLMNTYAEYDFFVLSSRFEGF
                     GMVLLEAMSCGLPCIAIDCPTGPREILDGGKYGILCDNQSNLGESIASLIVNKDLRTM
                     LSGKSILRSHDYNIQIISEKWQGLFESLNEKNIYEK"
     gene            12247..13176
                     /gene="wfdO"
     CDS             12247..13176
                     /gene="wfdO"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD37075.1"
                     /translation="MKKIYMKNNIALVLVTYNRESLLKEVLSSIKHLKDKPKHVYIID
                     NNSSDGTCNIIIDFIQQNSSILSMSYHNTGDNLGGAGGFAYGSRLAYADGFQWIWLAD
                     DDVVFEQTCLTQLMLYSSDADILQPMRINTDGSCAEISGIDYEINNIFRLNPKKLKIT
                     DIYEEKWDIQEIKTIPFEGPLIHRRVFEQIGFPNPDFFIFYDDLDFALRAQRAGFKIK
                     CVKSAHLIRKIRFVQSVALTSWKGYFMYRNFFKVQLTYARIPIGLFRVIAIFFIVIIY
                     SLLFGGAKNISTLISALRDALRKNFPLDQRYKP"
     gene            13193..14344
                     /gene="glf"
     CDS             13193..14344
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37076.1"
                     /translation="MKNKIAIVGAGLSGAVIARELADAGYLVDVFDKRQHIGGNCYTA
                     RDPQTNIMVHTYGPHIFHTNSKFVWAYINKLGVFKPFTNRVKAVTRGQVFSLPINLLT
                     INQFFNKLFTPAEAKKYIESISDKYIKDPNTFEEQALSFIGRELYEAFFKSYTIKQWG
                     ILPTDIPASILKRLPVRFNYDDNYFSHQYQGMPEDGYTPLFENLLNHSNINVILSTEF
                     SAIDCDKYIHTFYSGTIDGFFDYDLGRLLYRTLDFKTEVFWGDYQGCAVMNYCDNSKP
                     YTRITEHKYFSPWEQHDKTIIYKEYSRECNDSDIPYYPVRLVSDKEILKKYVERASKL
                     ENVTFVGRLGTYRYLDMDVTITEALNTAEQFLELHQNNQRIPAFFVDVL"
BASE COUNT     4969 a   2452 c   3214 g   5161 t
ORIGIN
        1 ctcctggtga ctcatgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcatatgaa
       61 ttagaatcgc tccttgaaca gcgtgtaaaa cgccagttgc tggcggaagt tcagtccatc
      121 tgcccaccgg gcgtcaccat tatgaatgtg cgtcagagcg aacctttagg tttgggccac
      181 tccattttgt gcgccagacc cgccattggc gacaatccat tcgttgtggt gctgcctgat
      241 gtcgttattg atgatgccag cgccgatccg ctgcgttaca accttgccgc tatgattgcg
      301 cgattcaacg aaacgggtcg cagtcaggtt ctggcaaaac gtatgccggg tgacctctct
      361 gaatactctg tcatccagac taaagaaccg ctggatcgtg aaggcaaagt cagtcgcatt
      421 gttgaattta tcgaaaaacc cgatcagccg cagacactgg attcagacat tatggcggtt
      481 ggtcgctatg tgctttctgc agacatttgg cctgaactgg agcggactca acccggtgca
      541 tggggacgta ttcagctaac tgacgcaatt gccgaactgg cgaaaaaaca gtccgttgat
      601 gcaatgttaa tgacgggtga cagctacgac tgcggtaaaa aaatgggcta catgcaggca
      661 tttgtgaagt acggactacg caacctgaaa gaaggagtga agttccgcaa agggattgag
      721 cagctgttaa gcgaataaaa atcttaccgg atgtaacggt taataagaaa atcataacgg
      781 cagtgaagat tcgtggtgaa agtaaattgc aacgaatgtt cctgccgtta ttgctttata
      841 aaacactata ataacaatga gttagcaata agattttagt caaatttttc caggattttc
      901 cttgtttcca gagcggattg gtaagacaat tagcgtttga atttttcggg tttagcgcga
      961 gtgggtaatg ctcgtcacat cgtaggtatg catgcagtgc tctggtagct gttaagccag
     1021 gggcggtagc gtgtgttaaa cttaaggggg ttgttgcgag taatcaagat cgcggcaatt
     1081 tcggattgtg tcttattttg ataataaaac tatcctgata actggtggta ctggctcatt
     1141 cggcaataag tttgtgcgca tgacattaga taaatataac cctaaaaaga ttattattta
     1201 ttctcgtgat gaaatgaaac agtgggaaat ggcaaaacat ttcaaggacg aaagccgaat
     1261 tcgattcttt attggggatg ttcgtgataa agatcgtttg tatcgtgcgt tggatggtgt
     1321 tgactttgtt gttcacgccg cagcaacaaa aatcgtacct acagcagagt ataatccctt
     1381 cgaatgtgta aaaacaaata tcaatggtgc gatgaatgtg attgatgctt gtatcgataa
     1441 aggtatcgaa cgtgttgttg cactttctac tgataaagca agcagccctg cgaatctgta
     1501 tggtgcaaca aaattagcat ccgataaact tttcgttgct ggtaactctt attctggtgc
     1561 gacaaaaact cgttttgctg ttgttcgcta cggaaatgta atgggatcac gtggttcggt
     1621 aataccgttc ttcttatcta tcaaagaaaa aggagaattg cctattactg atgatcgtat
     1681 gactcgcttt atgattactc tggagcaggg ggttgagtta gtttggcatg cgtttgaaga
     1741 tatggtcggt ggtgaaattt atgtaaaaaa aataccttcg atgaaagtaa ctgatatagc
     1801 aacagctgtt gcgcctaatg ctacacaaaa aatggtcggt attcgaccgg gcgagaaact
     1861 acacgagcag atgatcagtg ctgaagatgc ttattacact tatgaatatc cagagcattt
     1921 taagattctt ccagccattc acaactggtg taactcacct gaaaggatta aagatggtaa
     1981 aaaagtgcca gaaggcttcg tctatgaaag tgatagtaac gcagaatgga tgagtattga
     2041 agaacttcgt caatggatcg aggataatag agaaaaagta ggtaacattt gatgaaattt
     2101 attccctatg gacggcagga tgtttctgat gaagatatcc atgctgtggt ggatgtactg
     2161 aagtccgatt ttttgacgca aggaccatgt gttccccaat ttgagaaggc aattgctgat
     2221 tatgtgaatg ctaagtatgc agttgcagta aatagtgcta cttcagcact tcacattgct
     2281 tgtctcgcgt tggggatgaa gaaaggcgat tggctttgga catcgccaaa tacatttgtt
     2341 gcttctgcga actgtgctct ttattgcggt gctaatgtca gttttgtcga tattgatgcg
     2401 cggacttata atatgagtgt tagcgcgtta gaagcgaaac ttatgtctgc aaggaacaat
     2461 ggaacattgc ctaaggttgt tgttcctgtc gcatttgcag gacagtcatg tgaaatggag
     2521 gaaatatata aactctcaaa agagtatgga ttttcaatta tcgaagatgc ttcgcatgct
     2581 attggaggaa attaccaggg aaagaaaata ggtaattcgc gctttgcaga tattactata
     2641 ttgagcttcc atcctgttaa aattattaca actgcagaag gtggtatggc tctaaccaat
     2701 aatgatgatt tggcagaaaa aatgcagtta ttccgtagtc atgggatcac tcgtgatagt
     2761 aatcatatga cgaaggtaag tgaaggagga tggtattatc aacaagttga tcttggtctg
     2821 aattaccgaa tgactgaact gcaggctgca ttaggcgtga gtcagctgaa gagtattgat
     2881 gattttgtaa cacgccgcca tgaactggtt gaacgttaca aaaaagctct cgaagatatt
     2941 cctatttccc taccatacca ggcggaaagt ggtcacagtg cctttcacct ttatcctgta
     3001 accgttaaaa attctgcaca gcggaaaact ttgtttgatt atctgcggaa taaaaatatc
     3061 ggggttaatg tccattatat tccagttcat actcaaccgt attatgaaaa gttagggcat
     3121 aaaatcggag attatcctgt cgctgaggat tattactctc gaaccatcag cataccgatg
     3181 tattctgctt tgacaaatga agaccaggat tatgtaatcc aatgcattcg ggagtttttt
     3241 taaatgaatg tggcaatcat tccagctcgt ggtggtagta aacgtatccc tcgaaaaaat
     3301 attaaaatgt tttgcgggaa accaatgatt gcctggtcga ttaatgccgc tcgaaagagc
     3361 ggcgtgtttg acagaatcat tgtttcgacg gatgatatgg agattgcagc tgtagcaaga
     3421 aaattcggcg ccgaagttcc gtttatgcgg ccagaagaac tttcgaatga ttttactgga
     3481 acaatccctg ttatacggca tgcggctgaa tggcttattt ctcagggaaa taaaataaaa
     3541 tatatttgct gcatttatgc aacggccccc tttattagag atgaagattt aaaactaggt
     3601 ttgaacctta tcaaagcaaa ggggggggat tattgttttt ctgttactac ttattcgtac
     3661 cctattcaaa gagcattaaa aattaatgag aataacgaaa taaccatgtt tgatccgact
     3721 cagtttgaaa tgcgatctca agatcttgtg gacgcttggc atgatgccgg gcaattttat
     3781 tgggggaata ttgatgcatg gataaaagaa aaaccggtat ttaagtcaca ggctatacca
     3841 ttacctttgc ctagagaacg agtgcaagat attgatacgc cggaagattg gcatttcgct
     3901 gaggtgttat ataggataaa tacaataaaa aatgaagatt gtatttaggg ttgatgcttc
     3961 gctttcaatt ggttcaggac atgtttttcg ttgtctaaat cttgctggtg cattgagaaa
     4021 gaatggtttt gaatgcattt ttcttacaaa ggaacatgca ggaaacttaa ttcaatatat
     4081 aaagacatat ttgttccaag tatatgttat ttcaaaggaa gataatgttg atgataaata
     4141 tattgaaaat gaaaaagagt ggttaggagg gagtcaaaaa gaagacgctg aaaaaacttg
     4201 tgaaattatt tacagaaata attttgcacc aggcatattt attattgatc attattcatt
     4261 agattctgaa tgggaaagtt taattaaatg gaaatttcca agtacaaaaa ttgttgttat
     4321 tgacgacttg tgtaatagaa aacattattg tgatatcctg attgattcaa catttgagcg
     4381 tacttctgag gaatatagga gattagttcc tgaatattgt actattttgg ccggtactga
     4441 ctatgcttta ctgaaagatg aatttttgca attgcgcaaa gaagccatat taaaacgtac
     4501 atctatatta agccctcaga aaattttaat tactatggga ggggttgata tacataacgt
     4561 gactgggaaa gttttaaaag aattaaataa tagagctgat cttcattata aaaagatcac
     4621 tgttgtatta ggggtgaatt gccctcataa aaaaaacatt gaaaatattg cacaatcgat
     4681 aaaatatgat gtggatataa aagtgaatac caacaatatg ccacaactta tgttagaaca
     4741 tgatttatct attggagctt taggaagtac tacttgggag agagctgtgc ttggattacc
     4801 cacagtaaat attgctattg ctgacaatca actggttatt gtagagaaac taaggcgaaa
     4861 tggttttatt gtctttgata gcattaattt tactggggtg gaattagctc gttctttaat
     4921 aaaggtaact gatttctatg atgaaatggt gtctcgctcg ctctctatat gtgatggcag
     4981 gggattacaa aggacactaa aatttataat atctttagct acaaataaat aatgagacat
     5041 gcttatgact ggcaaaaata taaaaaaatt aaatttgctt aacattgttc attcttcgga
     5101 ggatattcaa gaaaaagttc gatgtataag aaatgaacct caaattagga agtggatgta
     5161 tacagatcat cttatatcaa aagatgagca taaaaattgg attgaaagat taagaaatga
     5221 atcaagaaat tttgtgtatg tcgtaatgaa tgactacgat gagcctattg gtgtcgttag
     5281 tgtaaattcg attgatttga aaaacaaaaa aggagactgg gcatattatt tgacagcaaa
     5341 tacacgtact ggtattggtg ctgtattaga attttttgtg attgattatg tttttaacta
     5401 tttgcatctt gaaaaattaa attgtgaggt aattgaacat aatgataccg tcgttcgatt
     5461 acatgagaaa tttgggttta agcaggaagg atttagagag tctaatatta taaaggatga
     5521 taagcgtttt ggagtttatt attttggttt aacgaaagaa aaatggatgc taacacgaga
     5581 atgtttaaag aataaatatt ataagctttt ttctcaatac gcggttgatt ttcattaata
     5641 ggtttatttt tggggtgata aatgaaagca attactattg caggacgtaa aattggtaaa
     5701 gattttcctc catatatcat tgctgaatta tcggctaatc ataatggaga tataaatcgt
     5761 gcatttaaga taatggaaga ggcaaaagca gctggagctg atgctgtaaa attacagaca
     5821 tatagagcag atactataac gctggattgt gattctgaag agtttcaaat acatggtggt
     5881 ttatggggtg gtcagacatt gtatagctta tatcaaagtg ctcaaatgcc ttgggaatgg
     5941 cataagccat tatttgataa ggcaaaagag ttaggcatta ctatttttag cagtcctttt
     6001 gattttactg ctgttgattt attagaagaa ttaggtgctc cggcttataa aatagcgtca
     6061 ttcgaagcta ttgatttacc gctaattcga tatgtagctc aaactgggaa accgatgatt
     6121 atctcgacag ggatggctga tgaacaggag attcaggagg cgatccatgt agctaaagaa
     6181 ggcggttgta aggagctcgt tgtcttgcat tgcgtaagtg gatatccggc tccaccagag
     6241 gattacaatc ttgccacaat ccctgacatg gctaaaaaat ttaacgttat aacaggtctg
     6301 tcagaccata caatagataa tactacggcg atagcatccg ttgcattagg agcgagtgtg
     6361 atcgaaaagc atgtaacact tgatcgtaat ggaggagggc cagacgatag tttctcactt
     6421 gaaccaagtg agcttacagt tctttgccgt gatgcaaaga tcgcttggca ggctttaggt
     6481 agaataaact ataccagaaa gccaagtgag atgggaaacg ttcaatttag acgttcattg
     6541 tattttgtta aagatatgaa agctggtgat attattgatg aaacatgtgt gcgcagtgtg
     6601 cggccaggct atggattacc acctaaatat tttgatgatt taattggtaa gcgagttatt
     6661 agtgatgtta tggccaatac ccgaacatca ttggaattaa ttgcagattt aaaatgaaag
     6721 ttattaaaga tagtgttctg tatgtaatag gtgagttgat atctaaaagt gttccatttc
     6781 tattgcttcc ctatcttaca aggcggttgg gaattgatgg ttttggagaa atgtcatttt
     6841 atcagacaat acttgcttta ttggttatcg ttataagctt agggcaagat ggggctgttg
     6901 ctagatattt ttatttctac ggagcccgtt ctataaattt gattgtgact gtaggtgctt
     6961 tatattcact tgtgacgaca tccctattgt ttttatcgtt ttcatttttt aaatccacga
     7021 tattatcaat tattgtgttg actgctggat ttcaatcact tttcaatacg cagctaagtc
     7081 ttaggcagtg ccaaaaagag tcagttcagt atattgtcat gcaaatatta gttgcgttga
     7141 cttctggtgg tataaccttt tgtttactgg aaatatatag tcatgattta gttgaaaaga
     7201 gatttattgc attgcttttg agtaatatag tttgtgtttt tattgcttat cttatttata
     7261 tgaggagacg gaaatataat cacgcatttt tatttcgacg atattatagt gcattgctat
     7321 acttgattgg ttttggaggc ccattgattt tgcatcaaat aagtggcttt cttaaaggac
     7381 aattagatcg agttttgata tatagccgtt tttctgcttc agatttaggg ggctattctg
     7441 ctgcatttca aatagcctca gttctcttga tactgataat ggcgttgaat aaagcactag
     7501 ttccatatta ttatagtgag ctcaaaaaaa agcatatagt ctttgatgat ataaaaaaaa
     7561 cttgcgtttt tttatttcta ttgattccac ttccatcaat catatcgttg attattcctg
     7621 ctgatttttg gtgttttatt ttaggaaatg aatttgttga tgttaaatat tacatcgcta
     7681 tattcctatt aggtataggt tttacaatac cttatctttt actagtgaat tatctttttt
     7741 attatggtaa aaataaaagt ataactatct gttcagtcat ttcaacatct atttattgca
     7801 ttgttcttta ttttttatca tgtttaggta tttcttatgt tccatattct atgttgatag
     7861 caaatatgct aatcgttcct ttactttata tggctgccaa aaaaacatca atgcttgaac
     7921 gccagtgaga ttaatatgcc ctcgttattt atatgtgtta cgccactgca aatgttgatt
     7981 gctgaaaaaa taattgataa aaccaggcca gtaaacatag aaattatagt gcttgcatat
     8041 cagaaaaatg ataaatatat gcattacata aagcggttgg agaaaaaatg tactaatttt
     8101 acagttttgg ctgtaacgcc aaaaaataaa tttgtaaccg tgatcgcctt tgcaaaatta
     8161 cataccatct taaataaaaa tatgagtaaa acttactctg aggtttatct gtctagtatt
     8221 gataacaagt atgtacaatt aattgtttct aagctcaatt atgcaaggtt atatacattt
     8281 gacgatggaa cagcgaatat aattaaatct agtgcatatt atcaagagga aaaaaaaact
     8341 ctcaagacta acattttgag atggattttt ggaattaata aaggattgca agaaattaag
     8401 agtgaaatat gcaagcacta tacaatttat ccaagtgtta gtaatattgt ctctaataca
     8461 gaattgatag agatgtttac acaatgcaaa aaaaggaagc aagataaaaa agttgtgaga
     8521 gtctttatag gacaaccttt tgatgaatta ggaataccat tatcactaat agaagagttt
     8581 ttctttaaat acaaaatgga ctattattat ccacacccaa gagagaaaat aattaataat
     8641 aagttcactt atatacactc acatttgatt tttgaagaat atataattga aggcttacaa
     8701 catcaagata tcgtatacaa aatatatggt gctgtgtgta caagtatatt aaatttagcg
     8761 tcctcgaaaa atgatatcga gatatgttcc atttatacgg atgagctgag aactaaatat
     8821 agtgattatt atgcactggc tgaaaaaatg aatattacct tgttaaaact tacttgatat
     8881 gataaaatat gactaatata acgttcagaa ctctgagaat tccaggatat ttactctcat
     8941 tatttgttct tttaatcgga tttataacat atttcatctt taatgaaact cttgcagcga
     9001 atttattagc agtgggggct attggcatta tcattcttga tagtttacat gataagcgga
     9061 ttttttattt aacacttttt cccgtgtatg tcctcgtagg tcaactcatt tcattattat
     9121 tagttgagca cggatggatt ctaatcgaac ttggaggtat taaaagctat cccattggtt
     9181 caattctttt tatggcgtta acaatagttt tattccattt tatcatattt ttaactgtaa
     9241 aaatttcaat attcaaattt gatcgaacat gtattgataa gttaagtggt aattctttta
     9301 tttattactt gccgattttt tatctatgct tagtatatat tcctgttttt atttatggct
     9361 ctgcattatc tgtaacaaat ggtaatcgag ttgtatacaa tcaaataata tcacctgttt
     9421 ttttatattt atttcaaatt aaacaattta tacttcctgt tgcaggattg tatttgctta
     9481 aaaataaaaa aatatttact atatatctgt ttgcaatttt actatggaat atattgattg
     9541 gagagaaggc aacgggcatt tggcagtcat tgtatccaat gctgttacct tatgttttga
     9601 taaattatga taaaataaaa acaaaaaata tattaatagt attaggtttt tgtattgtat
     9661 ttataaccag tagcatagta attaattata tatttattga aaagtcaggt gctacgttta
     9721 tatttgatag aatatcaatg caaggacaat tgtggtggta ttattttaat gagcacgttt
     9781 tgttagctaa gacccctcat gaattatctg aagagttctc gtcagaatat agtgggcttc
     9841 ttaatctgat gtatcattca atgcctgccc atcttttcaa tagctatatc gagcgcggtg
     9901 tagtcttgac gagtggtttc cccgcaatat ttctttttta ctttggacaa tactggctcc
     9961 ttcctacgtt attatcacct tttctttttg gtttagttgt ctattttttt tcaagaagtt
    10021 tatatagcgg tagtatattg agtttgctta tatcaagtaa actatttttt gcttttactg
    10081 ttttttttgc ccgtggagat atcgcaactt ttttagatta taaattgctt atttatctat
    10141 tgattataat aattttacaa tatttgccaa gggttaaggt ttaatatggc gtatacttct
    10201 cactttatat ataaagtagc taataagatt agcattttta taagaaaaat catcaactta
    10261 tttagattat ttctttacgg tgatgttgtt tcatatgatt ttgaaatgga ggagaataga
    10321 agaattatag tgtcaggaag tctaacaaag agagataaca tacctaaaat aatttggatt
    10381 tattggcatg atgagcaaat tccattatta atagaggctt gtataaaacg ccttaaagat
    10441 ttaaatccta attatcaatt aaatgtgcta aataaattta caattcatga atacttacct
    10501 gagagtctaa tgtggagaca ggatctttct gtccaaataa gatctgatct gattcgcttg
    10561 ggattattat ataaatttgg tggagtatgg atagatgcta gtgtaatgtt ttttgaggat
    10621 ctttcatggt tagaaagact ttcagcagag aataaatatg atttaatcgg tttttatcga
    10681 ggcagactat ctatcgactg ctataatcca atagtagaaa gttggatgct gggggcacca
    10741 aaatacaata agtttataaa gcgatggtat gacaactttt atccagtatc ttatttaggg
    10801 ttagatgaat atttcaacac aattaagaaa agaagtgatt ataaaattat caaacagaat
    10861 ataaatattc ccaaatattt atctgtttat ctggcacaac aaatttcgtc gcgagaaatg
    10921 agagattatt gcgcttattt aagaaaggta gaggatagtg catatttata tcaagagtca
    10981 tatccacagc gtgattgtgt tctctcggag gtttggtgta aaagaagaaa accacagaaa
    11041 attcctccac taatcaaatt aacaagccag aacagaaata caattttagc ctgtagtaaa
    11101 ataaatccac gtagtatcgt aggtgagttt ttagataata agaattagga atagagaaag
    11161 tgcgagataa aattgtattt gtagtcgctg atatcacggc tactggcggg attgaacgag
    11221 ttattactta tcttgccagt tatttatgta ataatgggta tagtgtagag atattatcta
    11281 ttcatagaag taatcgtaat ttaccttatc ctgttcatgc agacgttcat atctcttttg
    11341 ttgataaaac tataatgaag gcaagaaagc caggatctgt cagcaaatta ctttcacatt
    11401 ttaaatcctc atttcattta aatataaaat tatttaagag aagaaaatgt attattgtag
    11461 caaatagttt tcctgttgct ttattttcct gcttctcggc cttattttca tcaaaaatgc
    11521 tcgttgtcga gcatgttcat tatcactatt acagtaagtg cttagttaat gtaaggcgtt
    11581 tcatttataa gtttttttat ggggtagttg cgttaactga gcgtgacagt gagctttata
    11641 ttaatgataa actaaattct ctaacaatac ctaacgctct tagttctttt cctgaaaaaa
    11701 tagcgaatcc atctgtggaa aagaaaaaaa taattgccgc tggaagatta gaacatcaaa
    11761 aagggtttga tattctaata aaatcctttg ctgaaataga ctattctgtg aggaaagaat
    11821 ggatcatgga tatttatggg gatggaaatg aaagagctgc cttacagaaa ttaattcatg
    11881 atttaaatat tgaggattgt gttaatttat tgggtaacag tcgagacctt atgaacactt
    11941 atgctgaata tgattttttt gtattatctt caagatttga aggtttcggg atggttttac
    12001 ttgaagccat gagttgcggt cttccttgta ttgccatcga ttgtcctaca gggcctagag
    12061 aaatacttga tggaggcaag tatgggatac tttgtgacaa ccaatcaaat ctgggggaga
    12121 gtattgcatc tctaattgtt aataaggatc taagaactat gctatccggc aaaagcattt
    12181 tacgtagtca tgattataat attcagatta taagtgaaaa atggcagggt ttatttgaaa
    12241 gcttaaatga aaaaaatata tatgaaaaat aatatcgcac ttgttcttgt tacgtataat
    12301 agagagtctc ttttaaaaga agtcctttca tcgataaagc atttaaaaga taaaccgaaa
    12361 catgtttata taatcgataa taatagctca gatggaacct gtaatataat tatagatttt
    12421 attcaacaga attcatctat tctaagtatg agttatcata atacaggaga taatctcggt
    12481 ggggctggcg gatttgctta cggtagccgg cttgcttatg ccgatggatt ccaatggatt
    12541 tggttagctg atgatgatgt tgtttttgag caaacatgtc tgactcaatt gatgctttat
    12601 tcttcagatg ccgatatatt acagccaatg agaatcaata ctgacggtag ctgtgcagaa
    12661 atttcaggaa tagattacga aataaacaat atcttcaggt taaaccctaa aaaacttaaa
    12721 attactgata tatatgaaga aaagtgggat attcaagaaa tcaaaacgat accttttgaa
    12781 ggaccattaa tacatcgacg tgtatttgag cagattggtt ttcccaaccc cgattttttt
    12841 attttttatg atgatctaga ttttgcattg agggctcaaa gagcaggctt caagattaaa
    12901 tgtgtaaaat ctgctcatct gattcgtaag attagatttg ttcaaagcgt cgctttgacg
    12961 tcttggaaag gttattttat gtatcgtaac ttttttaagg ttcaacttac gtatgcgaga
    13021 ataccgatag gattatttag agttattgct atatttttta ttgtaataat ttattctcta
    13081 ctatttggag gtgcaaaaaa catttcgact ttgataagtg cattgcgtga tgcattaagg
    13141 aaaaattttc cacttgatca acgttataaa ccctaattta aaagttaaaa tcatgaaaaa
    13201 taaaatagct attgtcggtg caggcttaag tggggcagta atagctcgtg aacttgctga
    13261 tgctggatat ttagttgatg tttttgataa acgtcaacat attggaggta actgctatac
    13321 cgctagggat cctcaaacaa atattatggt tcatacatat ggcccacata tatttcatac
    13381 taacagtaaa tttgtttggg cttatattaa taagctgggg gtatttaaac cgtttactaa
    13441 ccgcgttaaa gctgttacta gaggacaggt tttttctttg cctattaacc tattaactat
    13501 aaatcaattc ttcaataaat tatttacacc agcagaagca aaaaaatata ttgaatctat
    13561 atctgataaa tatattaaag atcctaatac gtttgaagaa caagctttat cctttattgg
    13621 acgtgaatta tatgaggctt ttttcaagtc ttatacaatt aaacagtggg ggattttacc
    13681 taccgatata ccagccagta ttctcaaacg actgcctgtc cgttttaatt atgatgataa
    13741 ttatttttcg catcaatatc agggaatgcc ggaggacggc tatacacctc tattcgaaaa
    13801 tcttctgaat cactccaata tcaatgttat tctttctacg gaattctcgg caattgattg
    13861 tgataaatat attcatactt tttatagtgg cacaatcgat gggttctttg attatgattt
    13921 aggtcgtttg ctatatagaa ctctagactt taaaacggaa gtgttttggg gagattatca
    13981 gggatgtgca gtaatgaatt attgtgataa ttctaaacca tatacacgta ttactgaaca
    14041 taaatatttt tctccttggg aacaacatga caaaacaata atttacaaag aatatagtag
    14101 agaatgtaat gatagtgata ttccctatta ccccgtacga ctggtcagtg ataaagaaat
    14161 attaaaaaaa tatgtggaac gtgccagtaa acttgaaaat gttacttttg ttggccgatt
    14221 gggtacgtat cgatatttag atatggatgt aactatcact gaggccttaa acaccgccga
    14281 acagttttta gaacttcacc aaaataatca aagaatacca gctttttttg ttgatgtttt
    14341 atagcgagtc aacatgatta tattgtagta cataatatta cgttcatcct gataaattta
    14401 atctatattt tgctcatggc tttaaattta aagctattct tcatataata gcactaattg
    14461 cggtaacccc tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc
    14521 ggtgatgggg cgcaaccttg cgctcaatat cgaaagccgt ggttataccg tctctatttt
    14581 caaccgttcc cgtgaaaaga cggaagaagt gattgccgaa aattctggca aaaaactggt
    14641 tccttactat acggtgaaag agttcgttga atctcttgaa acgcctcgtc gcatcctgtt
    14701 aatggttaaa gcaggtgcag gcacagatgc tgctattgat tcccttaagc cataccttga
    14761 aaaaggcgac atcatcattg atggcggtaa caccttcttc ctggacacca ttcgtcgtaa
    14821 ccgtgagctt tctgcggaag gttttaactt cattggtacg ggggtttccg gtggtgaaga
    14881 aggtgcgctg aaaggtcctt ctatcatgcc tggcggtcag aaagaagcct atgaattggt
    14941 tgcgccgatc ctgaccaaaa tcgctgccgt agctgaagat ggcgaaccgt gcgttaccta
    15001 tattggtgca gacggcgcag gtcattatgt gaagatggtt cacaacggta ttgaatacgg
    15061 tgatatgcag ttgattgccg aagcttattc actgcttaaa ggtggcctga atctctccaa
    15121 cgaagaacta gcgcagacct ttaccgagtg gaataacggt gaactgagca gttacctgat
    15181 cgacataacc aaagacatct tcaccaaaaa agatgaagac ggtaactacc tggttgatgt
    15241 gattctggat gaagcggcta acaaaggtac cggtaaatgg accagccaga gtgcgctgga
    15301 tctcggcgaa ccgctgtcgc tgattaccga gtctgtgttt gcacgttata tctcgtctct
    15361 gaaagatcag cgtgttgccg cgtctaaagt tctctctggt ccgcaggcac agccagcagg
    15421 cgacaaagcg gagttcatcg agaaagttcg ccgtgcgctg tatcttggca aaatcgtttc
    15481 ttatgctcag ggcttctctc aattgcgtgc cgcgtctgaa gagtatcact gggatctgaa
    15541 ctacggtgaa atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc agttcctgca
    15601 gaaaatcacc gatgcctatg ccgaaaacct aaaaatcgct aacctgctgt tggctccgta
    15661 ttttaagaaa atcgctgacg actaccaaca agcactgcgt gatgtcgtcg cttatgcagt
    15721 gcaaaacggt attccggttc caaccttctc tgcggcggtt gcttattacg acagctatcg
    15781 tgccgctgtc ctgcct
//
