LOCUS       EU294163               15124 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella boydii O antigen gene cluster, complete sequence.
ACCESSION   EU294163
VERSION     EU294163.1
KEYWORDS    .
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:621"
                     /note="type: 8"
     gene            1574..2659
                     /gene="wfdP"
     CDS             1574..2659
                     /gene="wfdP"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdP"
                     /protein_id="ACA24747.1"
                     /translation="MKPYKLASHAIWESENKETILKLDWNESTIEPPERVKNALMNSL
                     VNNLIRYYPDVSNKDLIIKISNYVGVPKEYIQYFCSSDSAHESIIRTLINENDKILML
                     APTYDNFRTTGEAQGATICYSIFEDNYYWSLKKFESDLNKFQPQMAYLCNPNNPTGTL
                     IPKGDIHYLVEKYKGIYFIIDEAYYEFAKLTCADFVNSFNNIIITRTFSKAFSLAGMR
                     MGYIIASQDLLGAINKVRNAKNIPQLSQIAALNALDEVEYMNNYVKEVNQAKRFFYDK
                     LKNINIGQKNIVYGEGNFLVIEFKSMNDKKAFIERLKSNLIFVRDLSHMKEVELCVRI
                     TIGTKKQMLYVIRILEQMYEVKKDSII"
     misc_feature    1574..12934
                     /note="O antigen gene cluster"
     gene            2631..3314
                     /gene="wfdQ"
     CDS             2631..3314
                     /gene="wfdQ"
                     /note="unknown"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdQ"
                     /protein_id="ACA24748.1"
                     /translation="MKLKKIALFDLCETLVDFQTGDGFIYYVIDRLGICNNIKIQTLN
                     RVASSLSIFKIEQIINKIRLGESLRKRILLYMLKNIERDILNVMARDYVNEIILRRVN
                     PAIIKILNKHLKDGDEVIIISGGYDIYIQYLVKALEINSFVCTKIKFDNRGRCLGRIS
                     GKNCMGYNKVLMLKGVLSLDVSEYHITTYSDSISDLPILKMSNVGVVVSKGQAREWVR
                     QHKLFEILI"
     gene            3311..4228
                     /gene="wfdR"
     CDS             3311..4228
                     /gene="wfdR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24749.1"
                     /translation="MNRKNLSMKLMYILYNIKYLLKCFVVLITPDSLIIKKQFLEYQG
                     YPLGLNNPKTLNQKLQWLKLNDRTPLHTICADKIRVREYVENRLGTRDNIIDLLKVYD
                     TPFDITFESLPQGKFVIKSNHYCGDYFIVRDKNLLNIDEVKWKFIRTLNCNIYHHGRE
                     WQYKNIDRRIIVERLLEDEAGKVPNDIKIHCFNGEPKFIYVSLDREGGNYRSIYDTSW
                     NMLDFCWARRGKDLSKFNVKNISKPSQLENALNISKKLSQGFKYLRVDLYLVQEKIYV
                     GELTFHQGSGYDKITPFEWDERLGKELKL"
     gene            4225..4671
                     /gene="wfdS"
     CDS             4225..4671
                     /gene="wfdS"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdS"
                     /protein_id="ACA24750.1"
                     /translation="MSIFSRIQLIWYKLCFICEFGVKRYKQSHIYGPYKIVEPSNLKF
                     GNSFSINDFVYINASGGVILGHNVVLSAGSKILSTGLVFEKKIFKYKKDKVVIKDNVQ
                     LGAGAIVLPGVEICSNIIVGAGAIVTKNLEEPGVYVGQPARKIKDV"
     gene            4664..5869
                     /gene="wzx"
     CDS             4664..5869
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24751.1"
                     /translation="MSSFLRSIISVSALKLVDYIVPIIIMPLVLTRFNLEGYGVYSYV
                     LTISTFISFIYDACLNTVGVQQLNKLKGNEIAQQSFVISAIFFKLVFFFVCAIVFIII
                     SETAGKSDYFCVVIFSFGFGMLNSWYYIAKDNFRFIIIVSLVVKILAVLFVLYYAFEL
                     HMFIFIQAIASALVGLISLIKIKNDNRLGKISFRHIKNYLKNIKHMYIYSGVNALIQP
                     IINSAILASGNASLLGIYNVVMRFVAVSVTFSNSIITVLNKCNSEVFYSVGHKTNYVE
                     VKYRSQLLKLLFYSIVVFICVAAISYVSIYMTKGEISRFFIFLIFSFSLAVIPSILNQ
                     YLTQLIHMIHSSKNIVKYEICSNVLSLISIFLFSSLFPYYYIVYAVVLSPIIITICCL
                     WDLKCKNLL"
     gene            5851..6744
                     /gene="wfdT"
     CDS             5851..6744
                     /gene="wfdT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24752.1"
                     /translation="MQKLAVIMTYYNESYIILKKAVDSTLQDLERLEKKNVKYKLIIG
                     VDRRNEDIPHIESLYLKEVSARNDVCVVFFNENEGLAASLNKLIFGACNGYDFIARMD
                     ADDIVINGRFKKQLEYLNKHSDVSLVGGQAVVIDENDNVVRDYFNMNPVDFSWGNEII
                     HPTWLLRSELYYKLGGYRRLDAAQDYDFLCRAVLSGCIVRNIDDVVLFYRIRHGSIGS
                     CKKFIQIKNKTILSKFFRKNEVAPIHLFNATNFSVEFFLFSVIESIKIKHNIVGKCIG
                     WLSPLHAKNYYLNLRKRMGKK"
     gene            6741..7856
                     /gene="wfdU"
     CDS             6741..7856
                     /gene="wfdU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24753.1"
                     /translation="MRVLVFGPMISGHISNWIHGSKEYIDYRIVTLHYDKSISENVGI
                     GEIYKIPRLTKTKFDFIYAPVFLLFYFFLLKPELVHVHFFSSYGLISCVLPRKVKKIL
                     SVWGSDINNTIKKRGILGKIYVKAMKSFCVVNSPARHITNKIISYFNVNQERIHTFQY
                     GVDVGFFSDEKTSSIRDDKCITITSVRNWDNIYNIESFLEAVIDMKDYDCKGYFFNIN
                     ILGRSSSKTTHDKIIELTKKCEGDNYKVNLIGFIQKEELRSILHKSDFVLSIPSNDGA
                     PLSLMEGVLCNCFPIVSDIDANRELLGNHAMYLNLHDDIDMKSVFSRCVKLIDSNQYR
                     CSIEYNRDTIIKMFDINKNTKRMVDIYYRVVSQKEFL"
     gene            7853..8935
                     /gene="wzy"
     CDS             7853..8935
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24754.1"
                     /translation="MKVFFDVFLLFLFVFAFSIPVFFNSGYLIYIFSLIYLITNIRIR
                     TVFFSLISTRIFFIAFLSLACMLFFSCISLVLNGTGDLSIMGVIFNNLAALFSSVIIA
                     SHLIQHNNDNVLKLFFLIALLQSFFIILMMFFPSINAYIQSIIRTKEEIEFMSNTYGG
                     IRGLGISGSVAFGLAIIMSLLSFLSIVWFNEEDCNIPFFLKLFFIILLLFASISAGRT
                     AVLGFVLGGVYSFICSDYKKKFYRALQILIYVSLFLFIFWRFFLNEHVYDILKKYSDY
                     SFQMLYYYLDSGKLSTTSTDELFNMYFPLTEKQIFIGYGYHTVKGWDILSRDRCWIYA
                     VSIIFGCGTIGYFLFFVFYFFDYLCC"
     gene            10867..11880
                     /gene="gne"
     CDS             10867..11880
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACA24755.1"
                     /translation="MTILVTGGAGYIGSHTVLSLLSYGKNVVVLDNLSNSSSKSLERV
                     EKICGRRPLFYHGDVRDRKKLKHIFQYHDITDVIHFAGLKAVAESVSNPLEYYDHNVN
                     GTIVLLEEMMRAGVISFIFSSSATVYGKPETIPLKEDSSVGGTTNPYGFSKLIVERVL
                     KDIALAEPTMRITILRYFNPVGAHSSGLLGEDPNGVPNNLIPYITQVAIGNLKYLSVF
                     GNDYPTPDGTGIRDYIHVMDLAEGHIAALEHRNFGGNYKVYNLGTGVGYSVLDVINTF
                     KRCTGIDIPYCFKPRRVGDIAECWSDPSLAFLELGWSSRYDLDTMIIDSWRWQKNNPK
                     GYK"
     gene            12131..12934
                     /gene="wfdV"
     CDS             12131..12934
                     /gene="wfdV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24756.1"
                     /translation="MSTDKYLVSVITPTYNSERTIRCTIDSVLSQSYNNIEMIIVDDC
                     SLDNTVSICREYQKKDSRVVLISNEKNYGAGMSRNIAISKAKGRFIAFLDSDDFWHKE
                     KIKKQIDFMLSNNYAFTYTSYQKVSFSGKLLSQINPVSKVNYSELLKTNVIGCLTAVY
                     DTSSLGKMFMPTIRKRQDMALWLSILDKIDYAYCLNEILAYYRVGTDTLSSNKLKIIF
                     SQWAFYRKYLKFGLFKSLYYFSHYAVNAVAKHHINPLCLFFKRHFASWR"
BASE COUNT     4848 a   2110 c   2984 g   5182 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcagtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtaca gtccatttgc ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttatgtg cacgacctgc tattggtgac
      241 aacccatttg tcgcggtgct gccagacgtt gttatcgatg acgccagcgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaagtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agagccgctg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggctgtaggg cgttatgtgc tttctgccga tatttggcct
      541 gaactggagc gtactcaacc tggagcatgg ggacgtattc agttgactga tgccattgct
      601 gagttggcaa aaaaacaagc agttgacgca atgctgatga ctggggacag ttacgactgc
      661 ggaaagaaaa tgggttatat gcaagcgttt gtgaagtatg ggctgcgtaa cctgaaggaa
      721 ggcgctaagt tccgcaaagg cattgagaag ttgttaagcg agtaatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg ctgtgaagat ttgcggcgaa ggtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaaagag ttagcaatag
      901 gattttcgtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcatataag agaaaacatg
     1081 tggcactgat tttttactaa acaattggcg ataaatattc aataatggag attttcctta
     1141 acatcaaacg ctattatttt atatattata aatgtccaaa tactcagtat atatacaaaa
     1201 agagtaagat aaaacttaaa aatttaataa ggtgtctcta tatttctgac tacagacaag
     1261 catggaaagt ataatatatt gaatattctg tttctgatta cttatgctct caatttgcag
     1321 aagttagtga agatattcag tatgtggaat agatatatat tttttttgac acaatatgtc
     1381 gattattctt cacgagatta tattaagaat agaataaaat aaatgtatgg gcttatatac
     1441 gaattatgaa acataggagc aattctgaaa attatctaag ataagatatt gtgttttctt
     1501 gagtatgttt aaattcttta aatgttatgc acagaacgta atggagtaaa aattgaataa
     1561 gttcgtaaaa aaaatgaagc catataaact tgcatcccac gctatatggg agagtgaaaa
     1621 taaagaaaca atattaaaat tagattggaa cgaatctact attgaaccgc cagaaagagt
     1681 aaaaaatgca ttaatgaatt cgttggtaaa taatctaatt aggtattatc cagatgtgag
     1741 caataaagat ttaataataa agatatcaaa ctatgtgggg gtgccaaagg aatatatcca
     1801 gtatttttgt agttcagact cagcacacga gtctatcatt cgtacattaa ttaatgaaaa
     1861 tgataaaata ttgatgttag cacctacgta tgataatttc agaacaacag gtgaagctca
     1921 aggggcaaca atttgttatt caatttttga agataattac tattggtcat taaagaagtt
     1981 tgaaagcgat ctcaataagt ttcaacctca aatggcctat ttatgtaacc caaataatcc
     2041 tacaggtact cttattccta aaggagatat acattatctt gtagaaaaat ataaaggaat
     2101 atattttatt atcgatgaag cctattatga atttgcaaaa ttaacgtgtg ctgattttgt
     2161 aaactccttt aataacataa ttattacgcg aacattttcc aaagcatttt cattggcagg
     2221 gatgcgaatg ggatatataa tagcgtcaca agatttgtta ggtgcaataa ataaagtcag
     2281 aaatgcaaaa aatatacctc agctaagtca gattgccgct ttgaatgcat tagacgaagt
     2341 tgaatatatg aataattatg ttaaggaagt taatcaggct aagcgttttt tttatgataa
     2401 gttgaaaaat ataaatatag gtcaaaaaaa tattgtatat ggagagggaa actttttggt
     2461 tattgagttt aaatcgatga atgataaaaa agcattcatt gaacgtttaa aatctaattt
     2521 aatatttgtt cgtgatttat ctcatatgaa agaggtcgag ttatgtgtca gaattactat
     2581 tggaacgaaa aaacaaatgt tgtatgtaat cagaatttta gagcagatgt atgaagttaa
     2641 aaaagatagc attatttgat ctgtgtgaaa ccttagttga ttttcaaact ggcgatgggt
     2701 ttatatacta tgtaatagat cgtttgggga tatgtaataa catcaaaata cagactttaa
     2761 atagggtggc atcttcatta tccattttca aaatcgaaca aataataaat aaaataagat
     2821 tgggggaaag tttaaggaaa agaattttat tgtatatgtt aaaaaatatt gaaagggata
     2881 ttcttaatgt aatggctcgt gattacgtta atgaaattat attgcgcaga gttaatcctg
     2941 ccataattaa gatattgaat aagcatttaa aagatggaga tgaggttatt attatttctg
     3001 gaggctatga tatttatatt caatatttag ttaaagcatt agagattaat agctttgtat
     3061 gcacaaaaat taaatttgat aatagaggca ggtgtttagg gagaattagt ggcaagaatt
     3121 gcatgggata taataaagta ttaatgttaa aaggtgtttt gtcgttagat gtttctgagt
     3181 atcatataac aacttactct gattctatta gcgatttacc aatattaaaa atgagcaacg
     3241 ttggggtagt agtatcgaaa ggccaggcaa gagaatgggt aagacagcat aaattatttg
     3301 agattctaat atgaatagaa aaaatctttc catgaaattg atgtatatat tgtataatat
     3361 aaagtattta ttgaaatgct ttgtcgttct gattactcca gatagcttga ttataaaaaa
     3421 gcagtttctt gaatatcagg gataccctct cggattgaac aatccgaaga cattaaatca
     3481 aaagttacaa tggttgaaac taaatgacag aacaccatta catacaatat gtgctgataa
     3541 aattagagtg cgagaatatg tggaaaatag gctgggaacg agagataata taattgattt
     3601 gttaaaggtt tatgataccc cttttgatat tacctttgag tctttaccac agggtaagtt
     3661 tgttataaaa tcgaaccact attgtggcga ttattttatt gttagagaca agaatttatt
     3721 gaatattgat gaggttaagt ggaaatttat tagaacactt aattgtaata tttaccatca
     3781 cggaagagaa tggcaatata aaaatattga taggcgtata attgttgaaa ggttattaga
     3841 ggatgaggca gggaaagttc ctaatgatat aaaaattcat tgttttaatg gagaaccgaa
     3901 atttatttat gtgtcgttag atcgtgaggg agggaattat cgttctatat atgatactag
     3961 ttggaatatg ttagattttt gctgggcgag aaggggaaaa gatctttcaa aatttaatgt
     4021 gaagaatatt agtaaaccaa gtcagttaga aaacgctcta aatatatcta aaaaactaag
     4081 tcaaggtttc aaatatctta gggttgattt atatcttgtt caagaaaaaa tatatgtagg
     4141 cgaattaaca tttcatcaag gatcggggta tgataagata actccatttg aatgggatga
     4201 aaggctcgga aaggagttaa agctatgagc atattctcta gaattcaatt aatatggtat
     4261 aaattatgtt ttatctgtga gtttggagta aaacgttata agcaatccca tatttatggt
     4321 ccttataaga tagtagaacc atcgaatctt aaattcggta attccttttc aattaatgat
     4381 tttgtgtata taaatgctag tggaggagtc attttaggcc ataatgtagt actgtctgct
     4441 ggtagtaaaa ttctcagtac aggtttggta tttgaaaaga aaatctttaa atataaaaaa
     4501 gataaggtag taataaaaga taatgttcag ttaggtgctg gagcaattgt tttaccggga
     4561 gtggagatct gtagtaatat tattgttggt gccggagcga tagttacaaa aaacttagag
     4621 gaaccaggtg tatatgttgg gcaaccggct agaaaaataa aagatgtcta gttttttaag
     4681 aagtattatc tctgtaagtg ctctaaagct agtggattat attgtcccaa taataattat
     4741 gccgctggtt ttaactagat ttaatttaga gggatatgga gtttattctt atgtgttaac
     4801 tatatcaact tttatttcat ttatttatga tgcttgtcta aatacagtag gcgtacaaca
     4861 attaaataag cttaagggta atgaaattgc tcagcagtcg tttgttattt cagcgatttt
     4921 ctttaagtta gtgttttttt ttgtatgtgc gattgtgttt attataattt cagagactgc
     4981 agggaagagt gattattttt gtgtcgtaat attctcgttt ggatttggca tgcttaatag
     5041 ttggtactat atagcaaagg ataactttag gtttataata atagtgtcct tggtagttaa
     5101 aattttagcg gttttatttg ttttatatta tgcttttgaa ttacacatgt ttatttttat
     5161 tcaggcgata gcatctgctc tggttggact tattagtttg ataaaaataa agaatgataa
     5221 taggttggga aaaatatcat ttagacatat taaaaattat ttgaaaaaca ttaagcatat
     5281 gtacatatat agcggggtaa atgcacttat tcagcctata attaattctg ctatattggc
     5341 atcaggaaat gctagcttgt tagggatata taacgtcgtt atgcgatttg tggcagtatc
     5401 cgtaacattt tccaattcaa ttatcactgt cttaaataaa tgtaattcag aggtttttta
     5461 ttccgtaggg cacaaaacta actatgtgga agtaaaatac agaagtcagc tactcaaatt
     5521 gttgttttac agcatcgttg tttttatatg tgtggctgca atatcatatg tctctattta
     5581 tatgacaaag ggagaaatat ctcgtttttt tatttttcta atattttcat ttagcctagc
     5641 tgttatccca agtatactca atcaatattt aactcaatta attcatatga tacactcatc
     5701 aaaaaatata gtaaaatatg agatttgttc gaatgtacta tcgttaatat ctattttttt
     5761 attttcttca ttattcccat attattatat agtatatgct gtagttctat cgccaattat
     5821 tattacaatt tgctgtctgt gggatttgaa atgcaaaaac ttgctgtaat catgacttac
     5881 tataatgaga gttacataat actaaaaaag gcagtagaca gtactcttca ggatctggaa
     5941 agattggaaa agaagaatgt gaaatataaa ttaataattg gagtcgatag gagaaatgaa
     6001 gatattcctc atattgagag tttatattta aaagaagtta gtgccagaaa tgatgtgtgc
     6061 gttgtttttt ttaatgagaa cgaagggttg gcggcaagtt taaataaatt aatttttggc
     6121 gcgtgtaatg gctatgattt tattgccaga atggatgctg atgatattgt tattaacggg
     6181 cgttttaaaa agcaattaga atatctgaat aagcattctg atgtcagttt agtcggtgga
     6241 caggctgttg ttattgatga gaacgataat gttgttaggg attattttaa tatgaaccca
     6301 gtagattttt cgtgggggaa tgaaataatt catccaactt ggttgttaag gtcagagctt
     6361 tattataaat taggtggtta caggcgttta gatgctgctc aagattatga ttttttgtgt
     6421 agggctgtat tgagtggatg tattgtaagg aatatagatg acgtggtttt attttatagg
     6481 ataaggcatg gctcaattgg cagttgtaag aagtttatcc aaataaaaaa caagactatt
     6541 ttatctaaat ttttcagaaa aaatgaagtt gcacctattc atttatttaa tgcaaccaac
     6601 ttttcagttg agtttttttt attctctgtc attgaatcaa taaaaatcaa gcacaatatt
     6661 gtggggaaat gtattgggtg gttgtctcca ctccatgcta aaaattatta tttaaattta
     6721 agaaaacgaa tggggaaaaa atgagagtac ttgtttttgg ccctatgata tctggtcata
     6781 ttagtaactg gattcatggt tccaaagaat atattgatta tagaattgtc acactacact
     6841 atgacaaaag tatttctgag aatgttggaa taggagagat atataagata ccaagactta
     6901 caaaaactaa atttgatttt atatacgcac ctgtttttct tcttttttat ttttttttat
     6961 taaagcctga gttggttcat gtccattttt ttagttctta tggtttaatt tcatgtgttt
     7021 tgcctagaaa agtaaagaag atactctcag tatggggaag tgacattaac aatactataa
     7081 aaaagagagg gatattagga aaaatatatg taaaagcaat gaaaagtttt tgtgttgtaa
     7141 attcacccgc caggcatata acaaataaaa taatatcgta tttcaatgtg aatcaggagc
     7201 gaatacatac ttttcaatat ggtgtagatg tggggttctt ttcagatgaa aaaacgtcaa
     7261 gcataaggga cgataaatgt ataacaataa cttctgtaag aaattgggat aatatttata
     7321 acatagagtc ctttttagaa gctgttattg atatgaaaga ttatgattgt aaagggtact
     7381 tttttaatat caatatacta ggccgctcat cgtcaaagac cacacatgat aagataatag
     7441 aactcacaaa aaaatgtgag ggagataatt ataaagtaaa tttaataggg tttattcaaa
     7501 aagaggaatt aaggtcgatt cttcataaat ctgattttgt tcttagtatt cctagcaatg
     7561 atggtgctcc attatctttg atggagggcg ttttatgtaa ttgttttccg atagtatcag
     7621 acatcgatgc aaacagagag ctattaggaa atcatgcaat gtatttgaat ctgcatgatg
     7681 atattgacat gaaatctgtt ttttcaaggt gtgtgaaatt aatagatagt aatcaatata
     7741 ggtgttcgat tgaatataac cgagatacaa taataaaaat gtttgatata aataaaaaca
     7801 caaagcgtat ggttgatatt tattatcggg tcgtgtcaca aaaggaattt ttatgaaggt
     7861 tttttttgat gtatttttgt tgtttttatt tgtgtttgct tttagcatcc ctgttttttt
     7921 taattcaggc tatcttatat atattttttc cttgatttat ttaattacaa atatcaggat
     7981 aaggactgtt ttttttagtc taataagtac aagaatattt tttattgcgt ttttgtcttt
     8041 ggcctgcatg ctcttttttt cttgtatttc attggtatta aatggtacag gagatttatc
     8101 tatcatgggg gttatcttta ataatttagc tgcattattc agctctgtta taattgcgtc
     8161 gcatttaatc cagcataaca atgataatgt gttgaaattg ttttttctaa ttgccctctt
     8221 acaatccttt tttattattc tgatgatgtt ttttcccagt ataaatgctt atattcaaag
     8281 tattattaga acaaaagaag aaatagaatt tatgagtaat acatatggag gcatcagggg
     8341 attaggtata tcaggttctg ttgcctttgg tcttgctata attatgtcat tattatcttt
     8401 tctttcaatt gtttggttta atgaggaaga ctgtaatatt cctttctttt taaaattatt
     8461 ttttatcata ctcttgctgt tcgcgtcaat ttctgctggg cgtacggctg ttttggggtt
     8521 tgtcctcgga ggggtatata gttttatttg ctctgattat aaaaagaagt tttatcgggc
     8581 gcttcaaatt ttgatttatg tatctctgtt tttatttatt ttttggagat tttttttaaa
     8641 tgaacatgtt tatgatatct tgaaaaaata ttcggattat tcttttcaaa tgttatatta
     8701 ttatttagat tcaggaaagt tatcaacaac atctacagat gaattattta atatgtattt
     8761 cccgttaaca gaaaaacaga tcttcattgg ttatgggtac cataccgtta aaggatggga
     8821 catattatct cgggaccgat gctggattta tgcggttagc attatttttg ggtgcggtac
     8881 catcggttat tttttatttt ttgtttttta tttttttgac tatttatgct gctaatagca
     8941 tcaaacctaa aatagttgca ttaatgatta caatactcag tttttctttt cattataaag
     9001 gcgaggttat atttttgaat gttgcatata tgaaaataat atatatatat atattttcat
     9061 ctttttatat ttgtcataaa aaaagattaa taagaagggg ttcggttaat gcatgattat
     9121 tattttaatt gcaggttttt attaacaaac aacagtagaa actcgttaat tgttgacata
     9181 ttatcttatt ctagatatct gtaaaacaaa ttggtttttg cctttgtttt tatgggtatt
     9241 taaagactat agggtaatag cgcaacaatt taatttaagt tttttgagaa ctgtaagaat
     9301 agtgaactac atagatcaca aatctataac tgaaccaggt gataatccca tgaatacaat
     9361 ctataataaa taatgttttg gattagtaag tgttataaat ttacgtggaa attctatttg
     9421 cgtgtttatg ttgctatttc atggaatggt tttattgcca ttgctgtagt gttattttga
     9481 actaatttat ttggtgaaag attactgagt aatcttttga tactaaatcg attgtgtttt
     9541 atatctttga attaaatatt tttgccaaag cactccttca ctcttatata ttcagatact
     9601 ccttgtgtta taagagtaag gaatataaat tggaatatgg aaaaaggcgt gacatatttc
     9661 tgttgtcttt ggttatgtac acgatgggca gagtgtaagt tttcagagat gttgcaattg
     9721 tctccagata agaaaggaga gattgttatg ttgaaccaac atatcggcgt caggtgtttc
     9781 gggcattacc gtgcaacaat ctatgcgatg gaatacaatg ccatgcctga tattgcaaaa
     9841 ttaagaatga aaaccctgca ttttagggaa agacatggaa tcaatgcatc cgcagaaaat
     9901 ttttgtgtat caatacgcgt ttaatatata ggctcggaag tgcttatctc aagtagtaaa
     9961 tctttctagt atgtcttaca aaactatgac atccagaaat tttgaaagaa atatgatgaa
    10021 ttagaatagg acttctcaac cttggaaaag aacagatttt tgaccgtctg aatctgtagt
    10081 gtgaacagta gcatttggcc tgcccgagcg tctccactat tggaggaatg agctaggcag
    10141 cacatgataa aatgtggcta atactggtgt aactgggttc ccagacttca agtccaaggt
    10201 actattagcc cgctaaagac cggtgagctt atattgagct cagaatcggc gttatgttat
    10261 taccgtgatt gacgaacgca gcaactacgc tttggcggtg gcggtaacgt cgcttaacag
    10321 tgatgtaatc atgagtttct tctgccatca agctcggttg ttacctgttg gaatcaacca
    10381 gaatataccc gattatgggg aagagttcct gggaaaattt gacaaaaagc tacaggaagc
    10441 cgctatcagg caccaatagg cttatctcga tacgccgaaa atgaacacca tctgtgagcg
    10501 ctttaaccga tcactgagat agccgtttat tgagtttaat gaagaattgc tctttgaaaa
    10561 tctgaatatg tcaatcataa acaggctcaa tattgcatac tgtacaagaa taaaaggcct
    10621 cataaattgc tcgaattact gacaccacta gaacataatt tacacgaaaa aaatgcaata
    10681 tagtgttaga ctcatacaat atgtcgagat gtttagaaaa ataacgtata cttacatatt
    10741 gtatttttat agtaacattg ggcttaacta ttctaaattt ttattctttc acagtttata
    10801 ctatttataa tgtgtttata ttcttgtttt atatcctagg cagatagatg aaatataagg
    10861 agttttatga ctattttggt tactggtggt gcaggttaca ttggttcaca tactgtatta
    10921 tccttattat catacggaaa aaatgtggtg gtgttggata atctatcgaa ctcatcgtct
    10981 aagtcgttag aaagggtcga aaaaatttgt ggacgtaggc ctctatttta tcatggtgat
    11041 gtgcgtgata ggaaaaaact caaacatatt ttccagtatc atgatattac agatgttatt
    11101 cattttgcag gattaaaagc cgtcgctgaa tctgtttcaa atccattgga atattatgat
    11161 cacaatgtta acggaactat tgttttactt gaagaaatga tgcgcgctgg cgttattagt
    11221 tttattttta gctcctccgc aacagtgtat ggtaagcctg aaactattcc tttgaaggaa
    11281 gatagtagcg ttggcggaac aactaatcca tatggctttt caaaacttat agtagagagg
    11341 gttttgaagg atatagcttt ggctgagcca acaatgcgta ttactatttt gcgttatttc
    11401 aatcctgtag gagcccactc ttctggtcta ttaggtgaag atcctaatgg tgttccaaat
    11461 aatttaatcc cttatattac tcaagtggct attggtaatc taaaatattt atcagtattc
    11521 ggaaatgatt atcctacacc tgatggaaca ggcattagag attatattca tgttatggat
    11581 ttggctgaag ggcacatagc tgctttagaa caccgtaatt ttgggggaaa ttataaagta
    11641 tataatcttg gtacaggggt tggttattca gttcttgatg tgataaatac ctttaaacgt
    11701 tgtactggaa ttgatatccc ttattgtttc aaacctcgtc gagttggtga tatagctgaa
    11761 tgttggtctg atccatctct agcattttta gaattagggt ggtcttctcg atatgattta
    11821 gatacaatga taattgattc ttggcgttgg caaaaaaaca atcccaaagg atataaataa
    11881 gacgatttta atgtgctatt ttattcactg tcaatatttt tataaaacca tctttgcttt
    11941 gctttgcttt gctttgcttt gctttgcttt gtagttactc tcttaattaa cggcttttta
    12001 tattagggtg ggatgttaat gaaatacttg ataaatattt aatatgtgaa atttcttaat
    12061 aagctaccaa ggatggatat gataaacatg atattatata atgaaactta tgtaagtttg
    12121 agatgattct atgagtaccg ataaatattt agtttcagtc ataacaccca catataattc
    12181 tgaaagaacg attagatgta caattgattc tgttctatct caatcatata ataatataga
    12241 aatgattatt gtcgatgatt gttcgttaga taatacagtg tctatttgca gggaatatca
    12301 aaaaaaagat tcaagagttg ttctcatttc aaatgagaag aattatgggg ctggtatgtc
    12361 aagaaatata gcgatcagta aagctaaagg tcggtttatc gcttttttag atagtgatga
    12421 tttttggcat aaagagaaaa taaaaaaaca gatagacttt atgctttcaa ataattatgc
    12481 tttcacttat acctcatatc aaaaggttag ttttagcggg aaacttttat cccaaattaa
    12541 tcctgtttct aaagttaatt attctgaatt attaaagact aatgtaatag gttgcttgac
    12601 agctgtatat gatacttcat ctcttggtaa aatgtttatg ccaacaatca ggaaaagaca
    12661 ggacatggca ctatggctga gcattttaga taaaattgat tatgcatatt gtttgaacga
    12721 aatactggcc tactatcgag taggcaccga tacactctcg tcaaataaat taaaaataat
    12781 attttcacaa tgggcctttt acagaaaata tttaaagttt ggtctgttta aatctcttta
    12841 ttatttctcc cactatgctg ttaatgctgt tgccaaacat catataaatc cgctgtgttt
    12901 gttttttaag cgccactttg cctcttggag ataattcggc aaatcttata aagaaatatt
    12961 ggataatgct tttgatttgc aaatgtattt ttatatttga tgatgtcatg gatgagtttt
    13021 aagtgaattt caatagaata gggaaatgtg ggtctgtgtg gacaagaagt gtgattaatg
    13081 tttttgtatc agattataat ctgcaatcta tttagtaaaa ttatgtttac caagtgcctc
    13141 tcctgaagaa ttatttttat aaagccttta gttatcattg aaatgaattc tgaggttaat
    13201 tggtgcgaat gaacttagta ttatgataaa gattgtcttt attttactta aaagtataga
    13261 atagttcatt gcattatttg tgttgtataa aacttcagag tcaggaacag tcttaagttt
    13321 tattttttct aaattcattg gtggctatca tatggttatt agatgactgt gttaagatat
    13381 atttcattgg ctttaaggtt ttagtacata tgacgaatag aaccaatttg aatattctgg
    13441 aaacaccaac agcctcgttg tttgtcaaaa ttattttgag gttgataatt tgactctcga
    13501 ttattgtaat ttataaaata taagtgagtt gaaagatata tttgaggcat ggcagttaaa
    13561 tctcttatat atcgcggaga ctattaataa tagttagtct gagacactgg aaactagagt
    13621 gtggagagca acgttagttt tgagtatttt ctgtaattaa tttaatataa gtttaattac
    13681 atatacaatt atctgttgtt tgtcttatgc aatattttcc atatgacata tttttatatg
    13741 tgtttcaata cgtttattat tctgctaatt aaataggagt gaaaatgtca aaaaaacaga
    13801 ttggggttat cggtatggcg gtaatggggc gtaatcttgc actaaatatc gaaagtcacg
    13861 gctatactgt ttcaattttt aaccgttctc gtgaaaaaac agaaaaaatt atagctactc
    13921 attcggataa gaagttattt ccttactata caataaaaga gtttgttgaa tctctggaaa
    13981 cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct gctattgatt
    14041 ctctcaagcc atacctcgat aaaggtgaca tcatcattga tggtggtaac accttcttcc
    14101 gagacaccat tcgtcgtaac cgtgagctgt ctgcagaagg ctttaacttt atcggtagcg
    14161 gtgtttctgg tggtgaagaa ggtgcgctga aaggaccttc catcatgcca ggtggccaga
    14221 aagaagccta tgaactggtc gcaccgatcc tgaccaaaat cgccgcagtg gctgaagatg
    14281 gggagccatg tgttacctat attggtgccg atggcgcagg tcactatgtg aagatggttc
    14341 acaacggtat tgaatacgga gatatgcagc tgattgctga agcctattct ctgcttaaag
    14401 gtggcctgaa cctcaccaac gaagaactgg cgcagacctt taccgagtgg aataatggtg
    14461 aactaagcag ctacctgatc gacatcacca aagacaactt cactaaaaaa gatgaagatg
    14521 gtaactacct ggttgatgtg attctggatg aagcggctaa caaaggtacc ggtaaatgga
    14581 ccagccaaag cgcgctagat ctcggtgaac cgctgtcgct cattaccgag tctgtgtttg
    14641 cacgatacat ctcttctctg aaagagcagc gtgttgccgc gtccaaagta ctgtctggtc
    14701 cgcaagcgca gccagcaggc gacaaggctg agttcatcga aaaagttcgt cgtgctctgt
    14761 atctgggcaa aatcgtttct tacgcccagg gcttctctca gttgcgtgct gcgtctgaag
    14821 agtacaactg ggatctgaac tatggcgaaa tcgcgaagat tttccgtgct ggctgcatta
    14881 tccgtgcgca gttcctgcag aaaatcaccg atgcttatgc cgaaaatccg cagatcgcta
    14941 acctgctgct ggctccgtat ttcaagcaaa ttgccgatga ctaccagcag gcgctgcgcg
    15001 atgtcgtcgc ttatgcggta cagaacggta tcccggttcc gaccttcgcc gctgcggttg
    15061 cctattatga cagctaccgc gccgctgttc ttcctgcgaa cctaatccag gcccagcgcg
    15121 acta
//
