LOCUS       EU294178               15253 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae O antigen gene cluster, complete sequence.
ACCESSION   EU294178
VERSION     EU294178.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 15253)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 10"
     gene            143..1252
                     /gene="mnaA"
     CDS             143..1252
                     /gene="mnaA"
                     /note="UDP-N-acetylglucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="MnaA"
                     /protein_id="ACA24905.1"
                     /translation="MVVFGTRPEAIKMAPLVHEIKKNPELELKVCVTAQHREMLDQVL
                     DLFNIKPDYDLNLMKTGQTLNDITSSILIHIKSILKNYNPDIVLVHGDTATTFAVSLA
                     AYYEQIVIGHIEAGLRTGNIYSPWPEEANRKLTSVLAEYHFAPTETAKINLLREGCPE
                     YKITVTGNTVIDALHMVKDKIDKNLSLRAKLDNQFSFLKNDKKIILVTGHRRESFGRG
                     FENICEALALIAKNHDDVQIVYPVHMNPNVQEPVNRILSGIDNIHLIEPQQYLQFIYL
                     MDRSYLIITDSGGIQEEAPSLGKPVLVMRETTERPEAIKAGTVKLVGANKENIVREVN
                     ELLDCKEAYNAMSFAHNPYGNGTSSKIIISKLISS"
     misc_feature    143..13764
                     /note="O antigen gene cluster"
     gene            1256..2341
                     /gene="rmlB"
     CDS             1256..2341
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24906.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIKNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVLEHADICDAAAMARIFAQHQADAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTERTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2341..3240
                     /gene="rmlD"
     CDS             2341..3240
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24907.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVSAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWYEYASLIFSEARKAGIQLAIKKLNAIPTTCYPTPALRPLNSRLNTEKFQQCFD
                     LTLPMWNIGVKRMINELFTIEKI"
     gene            3293..4171
                     /gene="rmlA"
     CDS             3293..4171
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24908.1"
                     /translation="MTIRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFEQLLGDGSQWGLNLQYKVQPSPNGLAQAFIIGEEFIDG
                     DSCALILGDNIFYGHDLPKQLENAVAKDTGATVFAYHVTDPERYGVVEFDKSGTAISL
                     EEKPLIPKSNYAVTGLYFYDRGVVEMAKSLKPSVRGEYEITDLNRIYLEQGRLSVTMM
                     GRGYAWLDTGTHQSLIEASNFISTIEERQGLKVSCPEEIAYRKGFIDAEQVKLLAKPL
                     KKNTYGKYLLNMVKEL"
     gene            4174..4722
                     /gene="rmlC"
     CDS             4174..4722
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24909.1"
                     /translation="MNVIKTEIPEVLIIEPQIFSDERGYFFESFNQKLFDDVVNRHVE
                     FVQDNHSRSSKGVLRGLHYQLPPFAQAKLVRCIVGEVFDVAVDIRTMSPTFGHWVGVR
                     LSAENKRQLWIPEGFAHGFLTLSENAEFAYKTTNFYCKEAEAGIIWNDPQINIKWPED
                     NMEFLLSTKDVILPCLTDARHF"
     gene            4706..5866
                     /gene="wzx"
     CDS             4706..5866
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24910.1"
                     /translation="MLAISNIKMNKSILITFAGGLLSAVLTFFVNIIAAKYLIVREFT
                     QFAVFINSTVILSAVFEFGLSSSLVVLYKKKGRHYCEVLSSHAIFLMCILFIVISPIL
                     YAYWSIGGVICLLASFLITVHKILNVFNQIDELWGRYSFNNILCNFVKLNFVLFAVIL
                     HPENVIYALWALAIAYIVSIIYMISGRNIKLRLYKDGVNFRTEFNLLFKFYLITAIVA
                     LLMRVDVLIINYVGLNAADYFIASTLCMLIPLLNSSVTSVLVKVGTNHVRNKHKIRLI
                     IFIILGYLLCSKYVITILYDEQYKDATNIMYILAVAYAIGLYFTDKESELYHQSNNRL
                     LYLKISQLSVLAFGSFPLLFYMGETGMACAVLLSRLSGWVYLITMCKNEQKK"
     gene            5850..6917
                     /gene="wffS"
     CDS             5850..6917
                     /gene="wffS"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffS"
                     /protein_id="ACA24911.1"
                     /translation="MNKKNEITLWGYYGFNNLGDDVMLDVCLKEIKSTKKYETINVYG
                     NYQEIDKIDGVVFHPLAGIKNKVEFLKSILKSDKLIWGGGTCFYKDNNTGYSGLFLLF
                     VISLFCVLFRKKNFFLGIGLGKLNSSLSRVLTITTLSLSTLSIFRDVNSYNKALSLVN
                     PKKVGFGGDLVLLYDILERSNKFYGIERRYISVSGHYAYANDVGYIENFTCAIIKIAK
                     HHNIKDIVFVSMHQMPESNDHIFHKKLKTKLPDNFNLLFSDYVDSVNLLKNSTFHIGM
                     RLHSIVFASINSVPIVPFSYSPKINSFINTMFLDAKVFDGYSNIFNLSIDDLICKEIN
                     NENLNAEKDRALITIKHVLSF"
     gene            6933..8354
                     /gene="wzy"
     CDS             6933..8354
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24912.1"
                     /translation="MTLKDWRYIVFWAVFSFVWITFCADNVLGFIISVLMFVILLWYG
                     VFRKETILQVILLLLFLIPEYPRDILFLYDALDAKEIFYNTINTVKIGPLTLYYWAMI
                     SLPIVIIVKSGLKISYASGVAVSILIGVIIYLFLVTLTSSQSFNLSTAITNVKIYTIL
                     LCTFLLLSNWKEQELKAVLNNFLIFLPVIIGLRVILFITYDFTLGTPSWDYFTQPLIT
                     VIAFIYLLQNNNKTIIEKSLTYRILFYISLFSLSRALIAIVVISFIVAMFLSKKRVLK
                     MKVLIEVVPFITILCFTVLVTNPNLYNFLLWKVSDLNVFESKKEISGSGKVRQYEILN
                     ILDGTDSSDKTILIGFGLSGHFNFDKHKLPIQEALDSKSFSEVERLENRFYFPHSFLA
                     ANLLRGGILLTLIYVLVHLALAIHFYRRRDFYLSMLSIFLIYNSYVRVEYAILLSILI
                     LLALFTHKGRSINIDMIRQQPNE"
     gene            8347..9204
                     /gene="wffT"
     CDS             8347..9204
                     /gene="wffT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24913.1"
                     /translation="MSKVTLISTVFNEAGSIQRFCDSILIQTVIPDEIVIVDGGSTDD
                     TVSLIQNALSCIGKQCSLNVIVDPLCSKKYSSGPIARGRNVAIQNASYNQILVTDAGC
                     IVDKNWVKNMKACFDSGAEIVSGTYKARVTNKFQAYIADVFCPELPEYISIKQFLPSS
                     RSLGFTKDIWKKTGGYPEDSYTAEDTVFILRAFELTDKIKIAYDAFVYWDLPKDFFEL
                     KTKLISYGIGDGFHRLDKKKYFLRFVALVAFPLTILIMLLKRKKLIALPMYYYMLRGY
                     CKGLRGRSL"
     gene            11582..12697
                     /gene="wffU"
     CDS             11582..12697
                     /gene="wffU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24914.1"
                     /translation="MLKVLFVSRPTLFSGPGGDTIQLLKTKQYLENIGVEIDIFDGKD
                     VDIKEYDLVHFFNLRNPQDILHLVRKTKKFNKPMALSTIWGTYYECDQKARIGVQRWV
                     ANTFPESFVEYLKTAARIFKNRNFHAGTIEYILKGHKSSQKEIASIVDVLLPNSPTEL
                     ARVRRDMGLSGKRGISVANAVDLKVFDYNKVDTSKYQHFEGCLLSAARIEIRRCQLDL
                     IRACQGLPYKLVLVGKPSPNSIQYYEQCKKEAGDNVTFIEHVSQEELAALYKVAKAHA
                     LISWMETPGLSTLEAAVMNCNVLVTDRGDTEYYFQNMAVYCEPGDVASIRTGVINVME
                     KEFNDELKNRVVTNFTWEHTAQQTLEGYKLAIQLHQE"
     gene            12706..13764
                     /gene="wffV"
     CDS             12706..13764
                     /gene="wffV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24915.1"
                     /translation="MKKIAIIGTVGIPACYGGFESLVQNLVDYQSSDVSYTVFCSSKS
                     YHLKQNTYKSAQLKYIPLKANGISSIPYDIISLVRCLRIKYDVILILGVSGCMVLPLF
                     RLFSRSRVVTNIDGLEWRRDKWGKVTKAFLKFSERIAVKFSDVIIADNQAISDYVKSE
                     YNKDSIVIAYGGEHAVINDAVISDEKSDYYLSLCRIEPENNVSMILAAFANSRYKLKF
                     IGNWNNSEYGLLLKDKYSKFDNIEIIDSIYDIEQLFILRNMCKGYIHGHSAGGTNPSL
                     VEAMQFGMDIFAYDCSFNRYTTEQNAHYFSSSSELCKIIDEMEIGNIPKNGKIMSGIA
                     QYRYQWSIISRMYEDTYK"
BASE COUNT     4689 a   2578 c   3143 g   4843 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgggtaatta gattgagaca atccgttgaa
       61 tgcgagatta ttaggttctg ttgtaatcct ttaatcgtat tcaactataa ttaaagagat
      121 aacaaaagtg attaaaaaga taatggttgt ttttggtaca agaccagaag caatcaaaat
      181 ggctcctctt gtgcatgaaa taaaaaaaaa tcccgaatta gaactgaaag tatgtgtaac
      241 agcgcagcat cgtgaaatgt tggatcaagt tcttgatttg tttaatatta aaccagatta
      301 cgatctgaat cttatgaaga cgggtcaaac attaaatgat attacttcct ctattttaat
      361 tcacatcaaa tcaattctaa agaactataa tcctgatatt gtgcttgttc acggtgatac
      421 agcaactaca tttgcagtta gcttggctgc atattatgaa caaatagtca ttgggcatat
      481 agaggctggg ttgcggacag gaaatatcta ttcaccttgg ccagaggaag cgaatcgtaa
      541 gttaacatca gtgttagcag aatatcattt cgctccaacg gaaacagcaa aaataaattt
      601 attaagagaa gggtgtcctg agtataaaat tactgttact gggaatactg ttattgatgc
      661 tctacatatg gtaaaagaca aaatagataa aaatttaagt cttagagcaa aattagataa
      721 tcaattttct tttctaaaga acgataaaaa aataattctt gtcacaggtc atcgtcgtga
      781 aagttttggc agaggatttg aaaacatttg tgaagcatta gcattaattg caaaaaatca
      841 tgacgatgta cagatagttt atcctgtaca tatgaatcct aatgtacaag aacctgtgaa
      901 tagaattctt tcgggaattg acaatattca cttgattgaa ccgcagcagt acctgcagtt
      961 tatatactta atggaccgtt cttatctgat tataactgat tcgggaggta ttcaggaaga
     1021 agctccatct ttaggtaaac ctgttttggt tatgcgggaa accacagaac gtcccgaagc
     1081 tattaaggcg ggcacagtta aactggtcgg tgcgaataaa gaaaacatcg ttcgcgaagt
     1141 aaatgagctg ttggattgca aagaagctta taatgcaatg agttttgcac ataacccata
     1201 tggcaatggt acatcatcta aaataatcat ctctaaattg atatcttcat aaattatgaa
     1261 aatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa
     1321 aaatacgcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc
     1381 gcttgctgat gtttctgatt ctaaacgcta tgttcttgaa catgcggata tttgcgatgc
     1441 agctgcaatg gcacggattt ttgctcagca tcaggcggat gcagtgatgc acttggctgc
     1501 tgaaagccat gtggatcgtt ccattacagg ccctgcggca tttattgaaa ccaatatagt
     1561 tggtacttat gtccttttag aagccgctcg caattactgg tctgctcttg atagcgacaa
     1621 gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtatatggtg atttgcctca
     1681 tccagatgaa gtaaataata acgaagaatt acccttattt actgagagga cagcttacgc
     1741 gccaagcagc ccttattccg catcaaaagc atccagcgat catttagtcc gtgcgtggaa
     1801 acgtacctat ggtttaccga ccattgtgac taactgttcg aataactacg gtccttatca
     1861 ctttccggaa aaattgattc cactagtaat tcttaatgct ctggaaggta aggcattgcc
     1921 tatttatggt aaaggggatc aaattcgcga ctggctgtat gttgaagatc atgcacgtgc
     1981 gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa
     2041 cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc
     2101 gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgtccgg gacacgatcg
     2161 ccgttatgcc attgatgctg agaagattgg tcgcgaattg ggatggaaac cacaggaaac
     2221 gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa aatgggttga
     2281 taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta
     2341 atgaatattc tccttttcgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg
     2401 gcacctttgg gtaatttgat tgctcttgat gttcactcca ctgattattg tggtgatttc
     2461 agcaaccccg aaggtgtggc tgaaaccgta aaaaaaattc gtcctgacgt tattgttaat
     2521 gctgctgctc ataccgcggt agataaggct gagtcagaac ccaaatttgc acaattactc
     2581 aatgcgacaa gtgttgaagc gattgcgaaa gcagccaatg aggtcagtgc ctgggttatt
     2641 cactactcta ctgactacgt atttccggga acaggtgaaa taccatggca ggaggcggat
     2701 gcaaccgcac cgctgaacgt ttatggagaa accaagttag ccggagaaaa agcattacaa
     2761 gagcattgtg cgaagcacct tatttttcgg accagctggg tctatgcagg taaaggaaat
     2821 aacttcgcca aaacgatgtt gcgtctggca aaagagcgcg aagaactagc cgttattaat
     2881 gatcagtttg gcgcgccaac gggcgcagag ttgctagctg attgtacggc acatgctatt
     2941 cgtgttgcat taaataaacc ggaagttgca ggcttgtacc atctggttgc tagtggtacg
     3001 acaacttggt atgaatatgc ttctcttatt ttttccgagg caagaaaagc aggtatccaa
     3061 ttggcaatca aaaaattaaa tgctattccg accacctgct atccgacacc agcactgcgt
     3121 cctcttaatt cccgcttaaa tacggaaaaa tttcagcaat gtttcgactt gacattacct
     3181 atgtggaata tcggtgttaa acgaatgatt aacgaattat tcactataga gaagatttaa
     3241 cttactggtg ttttaagatt aaaaaacaat tcaccacata aagagtatat aaatgacaat
     3301 tcgtaaaggt attattcttg ctggtggttc gggtactcgt ctgtatcctg taaccatggc
     3361 ggtaagcaaa cagctcttgc caatatatga taaaccaatg atttattatc cgctatcaac
     3421 attaatgtta gcagggatta gagatatctt aattataagt accccacaag acacgcctcg
     3481 ctttgaacaa ttattaggtg atggtagtca atggggatta aatcttcagt ataaagtaca
     3541 gccaagccca aatggattag ctcaggcgtt tatcataggc gaagagttta ttgatggtga
     3601 cagttgtgca ctgattcttg gcgataatat tttttatggc cacgatttgc caaaacagct
     3661 tgaaaatgct gtagctaaag atacgggggc aacggttttt gcttatcatg tgactgaccc
     3721 agagcgctat ggtgtcgttg agtttgataa atctgggaca gctattagct tagaagaaaa
     3781 accattaata cctaaaagta actatgctgt tactggccta tatttttacg atagaggcgt
     3841 agtagagatg gctaagagtt taaaaccatc agttcgtggt gaatatgaaa taacagatct
     3901 taatcgaatc taccttgagc aaggtcgtct ttctgtaacg atgatgggac gcggttatgc
     3961 atggctggat accgggacac atcaaagttt aattgaagca agtaacttta tttctacaat
     4021 cgaagaacgt caggggttga aagtttcttg cccggaagaa attgcctaca gaaaaggttt
     4081 tattgatgcg gagcaggtta aactccttgc gaagccgcta aaaaaaaata catatgggaa
     4141 atatttattg aatatggtta aagaactatg agcatgaatg ttataaaaac tgaaattcct
     4201 gaagtactca ttattgagcc acagatattt agtgacgagc gtgggtactt ttttgaaagc
     4261 ttcaatcaga agttatttga cgatgttgtg aatcgccacg ttgaatttgt tcaggataat
     4321 cattcaagat cgagtaaggg agtgcttaga ggcttacatt atcaattgcc accgttcgcg
     4381 caagcgaaac tagttcgctg tattgttggc gaagtatttg atgtcgctgt tgatattcga
     4441 actatgtccc ctacgtttgg gcattgggtt ggtgtacggt tgtcagcaga aaataaacgt
     4501 caactttgga tcccagaggg ttttgctcat ggttttctaa ctttaagtga aaatgcagag
     4561 tttgcatata aaacaacgaa cttttattgt aaagaagctg aggctggtat aatctggaat
     4621 gatccacaaa ttaatattaa atggccagag gataacatgg aattccttct ctcgactaag
     4681 gatgtaatcc tgccatgctt gactgatgct cgccatttct aacataaaaa tgaataaatc
     4741 catattgata acatttgccg gtggcttgtt atctgcagta ttaacttttt ttgtaaatat
     4801 tattgcagct aaatatctaa tagtaagaga atttactcag tttgcagttt ttatcaacag
     4861 tacagtcata cttagtgctg tgtttgaatt tggtctttct tcctcattag ttgtgttata
     4921 caagaagaaa ggcagacatt actgcgaagt tctttcctca catgcaatat ttttgatgtg
     4981 tatattattt attgtaatat ctcctatctt atatgcatat tggagcattg gtggtgtaat
     5041 ttgtttactt gcttcatttt tgattacagt ccacaaaata ttaaatgttt ttaatcaaat
     5101 tgatgagctt tgggggcgat attcatttaa caatatactt tgcaatttcg ttaagctaaa
     5161 ttttgttctg tttgctgtca ttcttcatcc cgaaaatgtt atttatgcat tgtgggcttt
     5221 agctattgct tacatagtct ctattattta tatgatttct ggacgaaata ttaagctcag
     5281 actctataag gatggtgtaa attttagaac agagtttaat ttactgttta agttttatct
     5341 cattactgca attgtcgcat tgttaatgcg cgttgatgtg cttattatta attatgtagg
     5401 gctaaacgca gctgattatt ttatagctag tacattatgt atgttaattc ccctactcaa
     5461 ttcatctgtc acgtcagttt tggtaaaagt gggtacgaat catgtacgaa ataaacataa
     5521 gattagattg attattttta ttatattagg ttatctttta tgttcaaaat atgtaattac
     5581 aatactctat gatgagcaat ataaagatgc cacaaacatt atgtacattt tagctgttgc
     5641 gtatgccatt ggcctctact ttaccgacaa agaaagtgag ttatatcatc aatctaataa
     5701 tagattactc tatttaaaaa tatcccaact ttccgttctt gcatttggtt ctttcccact
     5761 tctattttac atgggggaaa caggaatggc atgtgctgtc ctcttgtcaa gactttcagg
     5821 ctgggtttat cttattacga tgtgcaaaaa tgaacaaaaa aaatgaaatt actctatggg
     5881 gttactacgg cttcaataat ctcggtgatg atgtaatgct tgatgtctgt cttaaggaga
     5941 ttaaatcaac taaaaaatat gaaactataa atgtatatgg taactatcaa gaaatagaca
     6001 aaattgatgg tgtagttttt cacccattag cagggataaa gaataaggtg gagtttctga
     6061 aatctattct taaatctgat aaattaatat ggggaggggg tacctgtttt tataaagata
     6121 ataacactgg atattctggt ttgtttcttc tattcgtaat ttctttattt tgtgtgttgt
     6181 tccgtaaaaa gaattttttt ctcggtattg gcttaggaaa attaaattca tccttatcta
     6241 gagtattgac cataacaact ttatcattga gcacattatc aatatttcgt gatgtaaatt
     6301 cttacaataa agcactgagt ttagtaaacc ccaaaaaggt tggttttggt ggtgatctgg
     6361 tattattata tgatatttta gaaagaagta ataagtttta tggaatagaa cggagatata
     6421 tttctgtttc gggtcattat gcctatgcta atgatgttgg atatattgaa aatttcacgt
     6481 gtgcaataat taaaatagct aaacatcata acattaaaga tatagttttt gtttcaatgc
     6541 accaaatgcc tgaaagtaac gatcatattt ttcataagaa attaaagact aagcttcctg
     6601 ataatttcaa tttattattt tctgattatg ttgattcagt taacttgctt aaaaattcta
     6661 cctttcatat tggcatgcgt ttgcactcaa tagtattcgc ttcgatcaat tctgtaccta
     6721 ttgtaccgtt tagctattcg ccaaagataa actctttcat caatactatg tttcttgacg
     6781 ctaaagtatt tgatggttat tccaatatat ttaacttgtc gattgatgat cttatttgta
     6841 aagaaattaa taatgaaaac ctgaatgctg aaaaagacag ggcattaatc actataaagc
     6901 atgtattatc tttttagata aatggatgaa atatgacact gaaagactgg cgttatatag
     6961 ttttttgggc ggtattttcc tttgtatgga ttaccttctg tgctgataat gtattaggat
     7021 ttattatatc agtattaatg tttgttatat tgttatggta cggggttttc aggaaggaaa
     7081 caattttaca agtaatactt ctgttgctat ttcttattcc agagtatcct cgtgatatct
     7141 tatttttata tgatgcgctt gatgcaaaag agatttttta taatacgatt aataccgtta
     7201 agattggtcc tttaactctt tattattggg caatgataag tttaccaata gtaatcattg
     7261 ttaaaagtgg gcttaaaata agttatgctt cgggagtcgc tgtttctata ttaattggtg
     7321 tgattatata tttatttctt gtaacactta ccagttcgca atcattcaat ttatcaactg
     7381 caattacaaa tgtaaaaata tatactatat tattatgtac attcttactt ctgtcgaatt
     7441 ggaaagaaca ggagttaaaa gctgtcttaa ataactttct aatttttttg ccagttatta
     7501 ttggtcttcg tgtgatattg tttatcacat atgactttac gcttggaaca ccttcttggg
     7561 attattttac gcaaccactt attaccgtta ttgcctttat atatttgcta cagaacaata
     7621 ataagaccat tatcgaaaaa tcattaacat accgtattct attctacatt tctctgtttt
     7681 cattaagccg tgcattaatt gctattgtcg taatttcttt cattgtggca atgtttcttt
     7741 caaaaaagag ggtgctaaaa atgaaagtgc tcattgaagt cgttcctttt attacgatac
     7801 tatgctttac agttctagtt actaatccta atttatataa ttttttactc tggaaagttt
     7861 cagatttgaa tgtttttgaa agtaaaaaag aaatttcagg ttctggaaaa gtaaggcaat
     7921 acgaaatact taatatactc gatggcactg atagtagcga taaaactatt ttgatcgggt
     7981 ttgggttatc tggtcacttc aattttgata aacacaaatt accaatacaa gaagctttag
     8041 acagtaaaag cttctctgaa gttgaacgat tagaaaatag attttatttc ccacattcat
     8101 ttttggctgc aaatctttta cgtggaggaa ttctcctgac tttaatttat gtcttggtgc
     8161 atctagccct tgcaattcat ttttatcgaa ggcgtgattt ctatttatca atgctttcta
     8221 tattcttaat ttataattct tatgtccgtg ttgaatatgc gatactatta tcaatactga
     8281 tattacttgc cttatttaca cacaagggac gttctataaa tattgatatg ataaggcaac
     8341 aaccaaatga gtaaagtgac attaatttca actgttttta atgaggctgg ttcaatccaa
     8401 cgtttttgtg attctatatt gattcaaact gttattcccg acgaaatagt cattgttgat
     8461 ggtggctcga ctgatgacac ggtatctcta attcagaatg ctttaagctg cattggaaaa
     8521 caatgtagtc taaatgtaat tgttgatccg ctttgttcca aaaaatactc aagtgggcct
     8581 atagctcgcg gcagaaatgt tgctatacaa aatgcatcct ataaccaaat tcttgtcact
     8641 gatgctggct gcatagttga caaaaattgg gttaaaaata tgaaagcgtg ttttgattca
     8701 ggagctgaga tagtttcagg aacgtataaa gctcgagtta cgaataaatt ccaagcatat
     8761 attgctgatg ttttttgtcc agagcttccc gaatatatat ccataaaaca gtttctccca
     8821 agctcccgtt ctttaggctt tacaaaggat atatggaaaa agacgggggg atatccagaa
     8881 gattcatata cagcagaaga taccgtattt attcttcgcg catttgaatt gactgacaaa
     8941 attaaaatag cctatgatgc atttgtttat tgggatctac cgaaagattt ttttgagcta
     9001 aaaacaaaac taattagtta tggtattggc gatggatttc atcggttgga taaaaaaaaa
     9061 tattttttaa gatttgtagc acttgttgca ttcccattaa cgatcctaat tatgcttctg
     9121 aaaagaaaaa aattgatagc actaccaatg tattattata tgttaagagg ttattgcaag
     9181 ggcctcaggg gtagaagctt atgaacatga ttcatgaaga aaactgtatt attccagtta
     9241 ttatggcggg tggctcagga gctcgttagt ggccctttca aggtctgctt atcccaagca
     9301 atttttgaaa ttgatgagtg ataaaattta ttgcaagata cactaagtag actagttgat
     9361 ataacgtccc gtaaaattat agttatgagt aatgagggct atcgttttat tgttaccggg
     9421 caattataat atattaacta taataatgtc gatattttac tcgaacctga agctaaaaat
     9481 acagcgctag caattgcata atccgctttt catgctttaa gttttgatgc tgatgcaatt
     9541 ctaatcgttt gggccgcaga ccattatatt agtgatacga ataaatttgc ttatatattt
     9601 aataagggta ttaaagtcgt atctgaagga tatattgtaa cattcggtgt attatctgat
     9661 aaagctgaaa ccggttacgg ttatattcgt tcgggagata aaataggcag taatctctat
     9721 aagattgatt catttgttga aaaaaactct attgagaaag ctacccatta tcttttgaag
     9781 gggcttatct atgtaacagt gtaatgtttg tatgcaaagc atctacttat cttaatgagc
     9841 taaagcgata tgctccggac atatattttg catgtgaaca atcaattgct agttcagcca
     9901 tggatatgga ttttaaccgg gttgatggca aggcgttttc acaatcgcca caaaattcta
     9961 ttgattatgc agggatgggg catacttcac taagtgctgt tatacccttt gattctacat
    10021 ggagtgacat aggtttatgg tcagggaaag atcacgtaac gctgcttttt tgtactaaat
    10081 aattcgcatt ttatgtttaa aaattgagat attcctcatt acttaaagct gttttttatt
    10141 acttagacat gatcaaatac tccttatata attaaggaga acaaaatgga acttaaaaaa
    10201 ttgatggagc atatttctat tatccccgat tacagacaag cctggaaagt ggaacataaa
    10261 ttatcggata ttctactgtt gactatttgt gccgttattt ctggtgcaga gggttgggaa
    10321 gatatagagg attttgggga aactcatctc gattttttga agcaatatgg tgattttgaa
    10381 aatggtattc ctgttcacga taccattgcc agagttgtat cctgtatcag tcctgcaaaa
    10441 tttcacgagt gctttattaa ctggatgcgt gactgccatt cttcagatga taaagacgtc
    10501 attgcaattg atggaaaaac gctccggcac tcttatgaca agagtcgccg caggggagcg
    10561 attcatgtca ttagtgcatt ctcaacaatg cacagtctgg tcatcggaca gatcaggacg
    10621 gatgagaaat ctaatgagat tacagctatc ccagaacttc ttaacatgct ggatattaaa
    10681 ggaaaaatca tcacaactga tgcgatgggt tgacagaaag atattgcaga gaagatacaa
    10741 aaacagggcg gtgattattt attcgcggta aaaggaaacc aggggcggct aaataaagcc
    10801 ttcgaggaaa aatttccgct gaaagaatta aataatccag agcatgacag ttacgcaatt
    10861 agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat
    10921 gaacttattg atttcacgtt tgaatggaaa ggactgaaaa aattatgcgt ggcagtctcc
    10981 tttcggtcaa taatagcaga acaaaagaaa gagcccgaaa tgacggtcag atattatatc
    11041 agttctgctg atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg
    11101 gagaataagc tgcactggcg tctggacgtg gtaatgaatg aagacgactg caaaataaga
    11161 agaggaaatg cagcagaatt attttcaggg atacggcaca ttgctattaa tattttgacg
    11221 aatgataagg tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga
    11281 aactatctcg cgtcagtcct tgcggggagc gggctttcgt aatcttgccc tggagaacct
    11341 cataagttga tgaataatct tgatatatca ataaatatgg gtaacagaga ttatactgat
    11401 gatgttagat tgaccttggt tgattcatct attattcaaa ttcgttcatc agtaaatacg
    11461 cctgaattaa gaatttattc agaatcttct agccaagaaa agtcaatgta ttacgtttct
    11521 tcgtgtatgt aacgattaat agattttgtt aagtgctgaa tattatctgg ggtaagaatc
    11581 tatgctgaaa gttttgtttg tttctcgacc tacattgttt agtgggcctg gaggtgacac
    11641 catacaatta ttaaagacaa aacaatatct tgagaatatt ggtgtggaaa ttgatatctt
    11701 cgatggaaag gatgtcgata tcaaggagta tgatttggtt catttcttta atctaagaaa
    11761 tccgcaagat attcttcatc ttgttagaaa aacaaaaaaa tttaataaac ccatggcgct
    11821 gtcaactata tggggaactt actatgaatg tgatcaaaag gcaaggattg gggtgcagcg
    11881 ttgggtcgct aatactttcc ctgaaagttt tgttgaatat ttgaaaactg ctgcgagaat
    11941 ctttaaaaat agaaattttc atgcaggtac tattgaatat atcttaaaag ggcataagtc
    12001 ctcccaaaaa gaaatagcgt ctattgtgga cgtattgctt ccaaatagtc caacggaact
    12061 tgctcgtgtt cgtcgcgata tgggattgtc cggaaaaaga ggaatatctg ttgctaacgc
    12121 tgttgattta aaagtttttg attacaataa ggttgatacc tcaaaatatc aacacttcga
    12181 aggatgcttg ctgagcgctg ctcgaatcga gattagaagg tgtcaacttg atcttatccg
    12241 agcttgtcag ggcttaccct ataaattagt cttagttgga aagcctagtc ctaatagtat
    12301 acagtactat gaacagtgta agaaagaagc cggagataat gttacgttca tagagcatgt
    12361 ttcgcaggaa gagttagcgg ccttatacaa ggtagccaaa gctcatgcat taatttcttg
    12421 gatggaaact ccggggcttt caacacttga agctgccgta atgaattgta atgtgctcgt
    12481 taccgatcgc ggtgacacag aatactattt ccagaatatg gctgtttatt gtgaaccagg
    12541 ggatgtggca tctattcgta caggtgttat aaatgttatg gaaaaggaat ttaatgatga
    12601 actgaaaaat cgagttgtta ccaattttac ttgggagcac acagctcaac aaacgctcga
    12661 aggctataaa ctagcaattc aactccatca ggaatgatta atagtatgaa gaagatagca
    12721 attattggta cagtaggcat tcctgcttgt tatggaggtt tcgaatcttt agttcagaat
    12781 ctcgttgatt atcaatctag tgatgttagc tacacagtat tttgctcatc aaaatcttac
    12841 cacttaaaac aaaatactta taaatcagct caactcaaat acatacctct taaggctaac
    12901 ggtatttcca gtatacctta tgatattatt agtttagtgc gttgccttcg aataaaatat
    12961 gatgtgattc ttatcttggg agtttctgga tgcatggtac tacctttgtt tcgtctgttt
    13021 tcaaggtccc gggtggttac aaatatagat gggctggagt ggcgccgtga taagtggggg
    13081 aaggtcacta aagcctttct caaattttct gaaagaattg ctgttaagtt ctctgatgtt
    13141 ataattgcag ataaccaagc tatcagtgat tatgtgaagt ctgaatacaa caaggatagt
    13201 attgttattg cctatggcgg tgagcatgca gtaattaatg acgctgtcat atcggatgaa
    13261 aaatcggatt attatctttc actttgtcgt attgagcctg aaaataatgt ctctatgatt
    13321 ctagctgcct ttgctaattc gcgttacaaa cttaaattta ttggcaattg gaataatagt
    13381 gagtatggtc ttttgttgaa agataaatat agtaaatttg acaatattga aatcattgat
    13441 tctatatatg acattgaaca gctatttata cttaggaata tgtgtaaagg atatattcat
    13501 gggcatagtg ctgggggaac taatccatct ctagtggaag ctatgcaatt tggcatggat
    13561 attttcgctt atgattgtag ttttaataga tacacaacag aacaaaatgc acactacttt
    13621 tccagttcgt cggagctatg caaaattata gatgaaatgg aaattggtaa tatcccaaaa
    13681 aatggtaaaa tcatgtcggg aattgctcaa tatagatacc agtggtcaat aattagtcgc
    13741 atgtatgaag atacctataa atagtgtctt ttaaaaggct ggcctaccat caatactgca
    13801 tcgttctttc cttaattcgt atcttccgct atccatattt atagatcctg agttaacatc
    13861 agtgaaaaca agcgtgtatt agtgcgcggt gacccccctg acaggagtaa acaatgtcaa
    13921 agcaacagat cggcgtcgtc ggtatggcag tgatggggcg caaccttgcg ctcaacatcg
    13981 aaagccgtgg ttataccgtc tctattttca accgttcccg tgagaagacg gaagaagtga
    14041 ttgccgaaaa tccaggcaag aaactggttc cttactatac ggtgaaagag tttgttgaat
    14101 ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc acggatgctg
    14161 ctattgattc ccttaagcca tacctcgata aaggtgacat catcattgat ggcggtaaca
    14221 ccttcttcca ggacaccatt cgtcgtaacc gtgagctctc tgccgaaggc tttaacttta
    14281 ttggtaccgg tgtttccggt ggtgaagagg gcgcgctgaa aggtccttcc attatgcctg
    14341 gtgggcagaa agaagcctat gagctggttg caccgatcct gaccaaaatc gccgcagtgg
    14401 ctgaagacgg tgagccatgt gttacctata tcggtgccga tggcgcaggt cactatgtga
    14461 aaatggttca caacggtatt gaatacggcg atatgcagct gattgccgaa gcctattctc
    14521 tgctaaaagg tggcctgaac cttaccaacg aagaactggc acagaccttt accgaatgga
    14581 ataacggtga actgagcagc tacctgatcg acatcaccaa agatatcttc accaaaaaag
    14641 atgaagacgg taactacctg gttgatgtga ttctggatga agcagcaaac aaaggtacgg
    14701 gtaaatggac cagccagagt gcgctggatc tcggcgaacc gctgtcgctg attaccgagt
    14761 ctgtgtttgc acgttatatc tcttctctga aagatcagcg tgttgccgca tctaaagtac
    14821 tgtctggtcc gcaagcgcag ccagcaggcg ataaggctga gtttatcgag aaagttcgtc
    14881 gtgctctgta tctgggcaaa atcgtttctt acgctcaggg cttctctcag ctgcgtgcgg
    14941 cgtctgaaga gtacaactgg gatctgaact acggcaaaat cgcgaagatt ttccgtgctg
    15001 gctgcatcat ccgtgcacag ttcctgcaga aaatcaccga tgcctatgcc gaaaatccgc
    15061 agatcgctaa cctgctgctg gctccgtact ttaagcaaat tgccgatgac taccagcagg
    15121 cgctgcgcga tgtcgtcgct tatgcagtac agaacggtat cccggttccg accttcgccg
    15181 ctgcggttgc ctattacgat agctaccgtg ccgctgttct gcctgcgaac ttaatccagg
    15241 cgcagcgcga cta
//
