LOCUS       EU294172               12449 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Shigella dysenteriae O antigen gene cluster, complete sequence.
ACCESSION   EU294172
VERSION     EU294172.1
KEYWORDS    .
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:622"
                     /note="type: 11"
     gene            50..445
                     /gene="wffW"
     CDS             50..445
                     /gene="wffW"
                     /note="glycerol-3-phosphate cytidyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffW"
                     /protein_id="ACA24835.1"
                     /translation="MKRIITFGTFDVFHVGHVNILERAASLGDYLIVGVSSDKLNFNK
                     KGRYPIYNQEDRCRIINSLRVVNDVFIEESLEQKKEYIIQYEADILVMGDDWAGRFDW
                     VNDICDVIYLPRTPSVSTTEIIEVVKTLR"
     misc_feature    50..12297
                     /note="O antigen gene cluster"
     gene            442..1602
                     /gene="wffX"
     CDS             442..1602
                     /gene="wffX"
                     /note="glycerophosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffX"
                     /protein_id="ACA24836.1"
                     /translation="MTNFKKIARKIISKSISLLNYFIPKKKNRIFFKSKPDYSGNCKA
                     LSDYIIEKKLPYHIVWSVKKEINQKGITVVRAGSLKEFFYYFTSKYVITTHNEMIGPI
                     ATNQKYISLWHGMPFKKICYLGENDHQGMIDYSAIRIATSEVMRSIISASFREKANNV
                     YITGQPRNDFLFKPISLTDIGIKSIKNKKIVMFAPTFRMNNEDIRYSDGAEIIDNNFL
                     RVNDFCMEEIDYYLEQSNLHLILKLHPYEEEYFRGIATLSSNITIISSDELTQKNIDL
                     NQLLSLVDILITDYSSIYLDYLILNKPLIFLVPDVDAYSSARGGFTLEPFDFWTPGDK
                     VSCQRSLLNSINKIITGNDEYAEKRNQINLIINKYSDANNSQRVIELMKSLS"
     gene            1599..2699
                     /gene="wffY"
     CDS             1599..2699
                     /gene="wffY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24837.1"
                     /translation="MKIYIFLGDLSSKGGIERVSVALANGLAKFYDVTIISLYRATRN
                     LSFVPDEKVNVIYLYDEFEKSMYNRNLGAIIGLKFDFLYIIKKLKQLKRLNLELNKND
                     VLISSDIKMSLLLFFYAKKSKIIAIEHFEHDVGNLVLRKIRSALYPKLSAVVSQTGED
                     QIKYLQWLPRKKHKIIPNIISFEATDIPQNKIEQKNVLAVGRLTHQKGFDLLLQAWAD
                     ANTHDWRLKIIGDGEELNHLNSLITELNISNAEIIPFQKDIQRHYSSAGIFVLSSRFE
                     GLGMVLLEALSSGLACISFDCPAGPKSIISSDNGVLVPTGDTIKLSQAISFLINNEEE
                     RKRLQNKAAASVEKFKESNVIAKWRELLNEIS"
     gene            2686..3741
                     /gene="wffZ"
     CDS             2686..3741
                     /gene="wffZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24838.1"
                     /translation="MKFHRKQYDAVFLTNVPAFYKVNLFNEIQKNKDIFVIFVSDKSK
                     IRNEDFYSYDFAFDHFFLNEENFEERSKIKTLFNLLRVLRTISYKKIIYSGWEVKEVT
                     LAALFNKREKNAIVIESSIIETKKTGLTWLLKKIAIGRMSLAYPSGLLQKAILETFSF
                     KGKTVITHGVGISNLKETSFHNKKTCTRNNPLRFIYVGRISSEKNIDFMVKVFKTLPY
                     ELILIGDGPLKKQFDDKTYSNIRFLGYIDNKKLSKELLKSDCFILPSLSEPWGLVVEE
                     ALTLGLPVIVSNHVGCHSDLVNDRNGIIFDVNDTQSFIDALSKMEKNYERFARGASEF
                     HASEIAKAQIDAYVGSI"
     gene            3741..5141
                     /gene="wzx"
     CDS             3741..5141
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACA24839.1"
                     /translation="MSNKNKTFKDFINYFLGDLFVKGFMFISLPLLSRIMSPEDYGRM
                     SLINAAVMILYVFISLNLQNAVINAYMKNEVDFPVYLGSVLWGLTAAQVLLVALSIYF
                     AVPLGMLLSISKYDVYWVVAICILLSYIYIYTSYLQGARLSSSFVKLNIFSKISEVVV
                     IFVFAWFLTQDKYLAKVYAQLVISFILLTYVLKKLKKIAVFKFNVRYFISALAFSSPL
                     IIHVLSNALLSQVDRLFIAKMLGEGQAGIYSFAYNIGMCILVVVMAWNSSWQPKLYKL
                     IDSKDNGKIIRIVDVSSLLLLIVSFLSILFSKQMVEVLADNRYRESISVVPVILIGNS
                     LIHIYLSYVNFTFYKKKTIYVSIGTLLAVAINIALNYILIPIYGIHGAAWATVIAYFM
                     LAFFHYLIATIMLKANPLSLFLLLWYSALLLASYFLVIYLDSLSLWISLSIKAMIIFI
                     ILIILMKTKIYNELKE"
     gene            5138..6301
                     /gene="wzy"
     CDS             5138..6301
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACA24840.1"
                     /translation="MTVSIYLLFLAFVFLLAMSDFFIIADARNRFLLYIILFSLLVTF
                     IGLRYQTGLDWLFYNNLFNGEGFSLAIEPGYYFFSYVSSFLMGYWIYQALITAVLIIC
                     LKTFFEKNTKNYLFCIGFFFLYQFIFVTETIRQIIALSIILVAYKKFYDGKKLQFHML
                     TILACSFHISAVIVFILIPFLKRRNIYILKILTIVGLVLAIFSVYPVDYLIQLLSLLP
                     AGGYIEKIRWYSQDDYAGSVLTFSLVFKVFVVLLFDYRFKSIKSHGQSLINARAYDFI
                     YTSVYLMIFMDVYLGRFGTISTRLDVYFIPCFLIALNHLINEHKQGVSRFIFFFVVMV
                     YFTINYLSIMNGYYFEKFYSPYQNYITEFLNPGSYSDRGWDVRYYFSNKELLQ"
     gene            6298..7407
                     /gene="wfgA"
     CDS             6298..7407
                     /gene="wfgA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24841.1"
                     /translation="MNLLINASNLYVGGGVQVAISVLEELSDSSFSFIAVVSPVVYSQ
                     LSDDAASCCIVIESSPSKLLNFKVRRQLDDIVKKNDISVVFTIFGPSYWSPKNVKHAI
                     GFALPWLIYDIEYIFKKLTLKAKLKFCILKLLQPYYFKKNADLIFTETDDVNLRVTKL
                     LNFEKEQVYTVSNTLNGLLKNSNCYDYSILDRLPTKEPNDIWLVTISHNYPHKNLEVI
                     KELVTVLPPCYKFILTVSSDFLQLVPKEHRERVITIGNATLSQCAPLYEVCDGLFMPT
                     LLECFSASYLEAMYMKKIIFTSDLPFAHTVCKDAAFYFAPHDVENIRSTLVNGFQNKE
                     ILNHKLNEGSKIYESFPSAKARALQYIDIIKSNLV"
     gene            7432..8466
                     /gene="fnlA"
     CDS             7432..8466
                     /gene="fnlA"
                     /note="4,6-dehydratase, 3- and 5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ACA24842.1"
                     /translation="MFKDKVLLITGGTGSFGNAVLNRFLETDIKEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYSSILSAARGVDFIYHAAALKQVPSCEFHPLEAVKTNVL
                     GTENVLEAAIANQVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVNSDKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKALTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIETLAIALKELLNVEQHPVNVIGTRHGEKLYEALLSREEMIAAIDM
                     GDYYRVPPDLRDLNYGKYVEQGDSRISAVEDYNSHNTQRLDVEGMKTLLLKLPFIRAL
                     RAGEHYDLDA"
     gene            8459..9571
                     /gene="fnlB"
     CDS             8459..9571
                     /gene="fnlB"
                     /note="reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="ACA24843.1"
                     /translation="MPNMKILITGADGFIGRNLCLRLQEAGYCDLVKIDRGSSAADLE
                     TGLQDADFVYHLAGINRPKNVDEFAEGNSNLTQQIVDCLLAKHKSIPIMISSSIQAEL
                     VNAYGQSKAAAEKHIERYAAESGAAYFIYRYPNVFGKWCKPNYNSFVATFCHNIANNI
                     DITINDSFAPVNLVYIDDVCSDAIKLLSGKVESGYKTVKPVYSTTVGEVAELLYRFKE
                     SRSTLVTEAVGTGFIRALYSTWLSYLPADMFAYSVPSYGDARGVFCEMLKTPSAGQFS
                     FFTAHPGITRGGHYHHTKNEKFLVIRGQACFRFEHVITGERYEMNVSSDEFKIVETVP
                     GWTHDVTNIGADELIVMLWANEIFSRDEPDTIARPL"
     gene            9571..10701
                     /gene="fnlC"
     CDS             9571..10701
                     /gene="fnlC"
                     /note="C-2 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="ACA24844.1"
                     /translation="MKKLKIMSVVGTRPEIIRLSRVLAKLDEHCEHILVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLESEKPEAMLVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGL
                     PADRIIKTGSPMFEVLTYYMPQIDNSDVLSRLNLRSGEFFVVSAHREENVDSPKQLVK
                     LATILNTIAEKYDLPVIVSTHPRTRNRINEQGIEFHPNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLDCERVLQALDILATQ
                     PRGETRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            10701..11912
                     /gene="wbuB"
     CDS             10701..11912
                     /gene="wbuB"
                     /note="L-fucosamine transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACA24845.1"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIRRGHDVTVITPDIC
                     LQDDVSFSTFQGVKTWRFKSGPLKDVSKIQRAINETLLSYRAWNSIKSQIKKETFDGV
                     VYYSPSIFWGHLVKKIKSRCQCPAYLILRDMFPQWVIDAGMLKAGSLIERYFRVFERS
                     SYRQANRIGLMSDKNLEVFRVNNKGYPCEVLRNWASLTPTVPPQGYIPLRQRLGLDDK
                     VIFFYGGNIGHAQDMGNLMRLARKMAEHPQAHFLFIGQGDEVELINSLAAEWSLPNFT
                     YLASVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DVVNQNNAGLIHINGEDDKLHESALLMLKDVAARRQFGLGANALLREQFSVESAAQTI
                     EMRLEACNATH"
     gene            11899..12297
                     /gene="wbuC"
     CDS             11899..12297
                     /gene="wbuC"
                     /note="unknown"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ACA24846.1"
                     /translation="MRLIDTHQLEALYEQAGKSARLRAHLLLHNSHREKVQRLLIALV
                     QGSYVDPHFHELPHQWEMFVVMQGQVQVCLYGKDGEIINQFVAGENTAISVVEFSPGD
                     IHSVECLSPRALMMEVKEGPFDPSFAKAFI"
BASE COUNT     3818 a   2096 c   2462 g   4073 t
ORIGIN
        1 attggtagct gtaagccagg gcggtagcgt atttaattgt gagagaacaa tgaagagaat
       61 tattacgttt ggtacatttg atgtttttca tgttggtcat gttaatatcc ttgagcgagc
      121 tgcttcactt ggcgactatc ttattgtagg tgtaagttca gataaattga atttcaataa
      181 aaaaggtcgt taccccattt ataatcaaga agaccgctgt cgtataataa attctcttag
      241 agttgtgaat gatgtattta ttgaagaatc tttagagcaa aaaaaagagt atataatcca
      301 atacgaagct gatattttag ttatgggtga tgattgggct ggccgatttg attgggtaaa
      361 tgacatttgt gatgttattt atttaccaag aacaccatca gtatctacca cagaaattat
      421 tgaagttgtg aagaccctca gatgacgaat ttcaaaaaaa tagcaagaaa aataattagt
      481 aaaagcattt cattgttaaa ttattttatc cccaagaaaa aaaatagaat ttttttcaaa
      541 agcaaacctg actactcagg taattgtaag gcactaagcg attatattat agagaaaaaa
      601 cttccatatc atatcgtttg gtctgttaaa aaagaaatta atcaaaaagg aattactgta
      661 gtaagagctg gttcattgaa agaatttttc tattacttta caagtaaata tgttattact
      721 acgcataatg aaatgatagg tccaattgca acaaatcaaa aatacataag cttgtggcat
      781 ggaatgcctt ttaagaaaat ttgttatctt ggagagaatg atcatcaagg aatgatagat
      841 tattcagcca ttaggattgc cacttccgaa gtaatgcgtt ctataatttc agcaagcttt
      901 cgtgaaaaag ccaataatgt atatattaca ggccagcctc gtaatgattt tttgttcaaa
      961 ccaattagtc tgacagatat tggtattaaa tctataaaga acaaaaaaat tgtaatgttt
     1021 gctccaacat ttcgtatgaa taatgaagat ataagatatt ctgatggagc ggaaattatt
     1081 gataataatt ttcttcgagt aaatgatttc tgcatggaag agatagatta ttatcttgaa
     1141 caaagcaatt tacatttgat attaaaattg catccatacg aggaggaata tttccgcggg
     1201 atcgcaacac taagttcgaa tataactatt ataagttctg atgaactcac gcaaaaaaat
     1261 atcgatttga atcagttgct ttccttggtc gatattttaa taacagatta ctcatcaatt
     1321 tatttagatt acttaattct aaataaacct ctaatttttt tagttcctga tgtagatgct
     1381 tatagttctg cacgcggtgg gtttacttta gaaccttttg atttttggac gccaggagat
     1441 aaggttagct gtcaaaggtc attattgaat tcaataaata aaataatcac tggaaatgat
     1501 gagtatgctg aaaaacgtaa ccaaataaat ctcataataa ataaatactc tgatgctaat
     1561 aatagccaga gagtcattga attgatgaag agtttatcat gaaaatatac attttcttag
     1621 gagatttaag ttcgaaaggt ggtattgagc gtgtttcagt agcattagcc aatggattgg
     1681 ctaaatttta tgatgtcact atcattagct tatatcgtgc aactagaaat ttatcatttg
     1741 tcccggatga aaaggtcaat gtgatctatt tgtacgatga atttgaaaag agtatgtata
     1801 atcgtaatct gggagctatc atagggttaa agtttgattt cttatatatt ataaaaaagt
     1861 tgaagcaact taaaagatta aatttagaac taaataaaaa tgatgtgctt atttcaagcg
     1921 atattaaaat gtctctgcta ttatttttct atgcaaaaaa aagcaaaatt atagctattg
     1981 agcattttga acatgatgtt ggtaatttag tgttgagaaa aattagaagc gcactatatc
     2041 ctaaattatc agcagttgta tcgcaaactg gcgaagatca aattaagtat ttgcaatggt
     2101 tacctagaaa gaaacataaa atcattccaa atattattag cttcgaagcg accgatatac
     2161 cgcaaaataa aatagaacaa aaaaatgtac ttgctgtggg acgattaact catcaaaagg
     2221 gttttgattt acttctacaa gcttgggcag acgcaaatac tcatgattgg cgcttaaaga
     2281 tcattggaga cggagaagag ctgaaccatt taaattctct aattaccgag ttaaatatct
     2341 ctaacgctga aattatccct tttcaaaagg atattcaaag gcattattct tctgcaggaa
     2401 tattcgtact ttcttctcgc tttgagggtt tgggtatggt gcttttagaa gctcttagca
     2461 gcggcttggc gtgtattagt tttgattgtc cagctggtcc taaaagtata atctcaagtg
     2521 ataatggggt gttagttcca actggtgaca ctataaaatt atcacaagct atttctttct
     2581 tgataaataa cgaagaagaa agaaaacggt tacaaaacaa agctgctgct tctgttgaaa
     2641 aattcaaaga atcaaacgta attgcaaaat ggcgagagtt attgaatgaa atttcataga
     2701 aaacaatatg atgcagtatt tcttacaaac gtacctgctt tctataaagt aaatttgttc
     2761 aacgaaattc aaaaaaataa agatattttt gttatattcg tttcagataa gtcgaaaatt
     2821 aggaatgaag atttttattc atacgacttt gcgtttgacc acttttttct taatgaagag
     2881 aactttgaag aacgaagtaa aataaaaaca ctattcaatt tacttagagt gttacgaacg
     2941 atttcgtaca aaaaaatcat atattcagga tgggaagtta aagaagtaac tctagctgca
     3001 ttatttaata agcgggaaaa aaatgcaata gtgatcgaaa gtagcattat tgaaacgaag
     3061 aaaactggtt tgacatggct tctaaaaaaa atagctatcg gtcgtatgtc gcttgcttac
     3121 ccatcagggc tattgcaaaa agcaatattg gagacattct cttttaaagg aaaaactgtt
     3181 attacccatg gcgttggtat ctctaattta aaagagacca gttttcataa taaaaaaaca
     3241 tgtacaagga ataatcctct tcgtttcatc tatgttggaa ggatttcatc ggaaaaaaat
     3301 atagatttta tggtgaaagt atttaaaact cttccttacg aattgatact aattggagat
     3361 ggtccattaa aaaaacagtt tgacgataaa acctatagca atatcagatt tttaggctat
     3421 attgacaata aaaaattatc aaaagaacta cttaaaagtg attgttttat tcttccgtca
     3481 ttatctgaac cgtggggatt agtagtagag gaggctttga cattagggct tccggttata
     3541 gtcagtaacc atgtgggctg tcatagtgat ttagtcaatg atagaaatgg cattatattc
     3601 gatgtgaacg atacacaatc ctttattgac gcattgtcga aaatggaaaa aaattatgaa
     3661 cgctttgccc gtggtgccag cgaattccat gctagtgaaa tagcaaaagc acagatcgat
     3721 gcttatgtag gtagcatctg atgagcaaca aaaacaaaac atttaaagat tttataaatt
     3781 attttttagg cgatcttttt gtcaaaggat ttatgtttat ctcgctacct ctactttcga
     3841 gaataatgtc gccagaagat tatgggcgga tgtcccttat aaatgcagct gttatgattt
     3901 tatatgtttt tataagttta aatctccaaa atgctgttat caacgcttac atgaaaaatg
     3961 aagttgattt tccagtatat ctaggtagtg tattatgggg attgaccgcc gcgcaagtat
     4021 tgttagtcgc tttaagtata tattttgctg ttccgcttgg aatgttattg agtattagca
     4081 aatatgatgt ttattgggtt gtcgccattt gtattttgct aagctatata tatatatata
     4141 ctagctatct acagggtgca cggctcagtt ctagctttgt caaactgaat atatttagta
     4201 agatatcaga agttgttgtc atttttgtct ttgcttggtt tttaactcaa gataaatacc
     4261 ttgctaaggt atatgctcag ttagtgatta gctttatttt actgacatat gtgttgaaga
     4321 aactaaaaaa gatagctgta ttcaaattta atgtacgata ttttatatca gcacttgcat
     4381 ttagttctcc attaattata catgttcttt caaatgcttt gctttcacaa gttgaccgat
     4441 tgtttatcgc aaagatgttg ggagaggggc aggctggtat atattctttc gcatataata
     4501 tcggaatgtg tatattagtg gttgttatgg cctggaactc ttcgtggcaa cctaaattat
     4561 ataagcttat tgattcgaag gataatggta aaataattcg aattgtcgat gtaagttcct
     4621 tattattatt aatagtatca tttttatcta ttcttttttc aaaacagatg gttgaagtcc
     4681 ttgcggacaa tcgctatagg gaaagtatat ccgtcgttcc tgtcatattg attggtaact
     4741 ctctgattca tatttattta agctatgtta actttacttt ctataagaaa aaaacaatat
     4801 atgtttcaat tggtacattg cttgcggtag cgataaatat tgctttgaat tatatactca
     4861 taccaatata cggtatccac ggagcagcat gggctacagt aatagcttat tttatgctgg
     4921 catttttcca ttatctcata gcaacaataa tgttaaaggc aaacccactc tcattgtttc
     4981 tgttattatg gtattcagct ttgttattgg cttcgtattt cttagtaata taccttgact
     5041 ctttgtctct ttggatctct ttatcaataa aggcaatgat catttttatt atcctcatca
     5101 tccttatgaa aacaaaaatc tataatgaat taaaggaatg acagtgtcga tatatcttct
     5161 gtttctagct ttcgtatttc tgcttgcaat gtcagatttt tttatcattg ctgatgccag
     5221 aaatcgtttt ttactttata taatactatt ttcgctatta gtaaccttta taggtttaag
     5281 atatcaaact ggacttgatt ggttatttta taacaatcta tttaatggag agggtttttc
     5341 attagccatt gagcctggat attatttttt ctcgtatgtt tcatcttttt taatggggta
     5401 ttggatttat caggcattaa taaccgccgt tctaataata tgtttaaaaa cattctttga
     5461 aaaaaacact aaaaactatc tgttttgcat aggttttttc ttcttatatc aatttatttt
     5521 cgtgacggaa acaatacggc aaataatcgc tctgtcaatt attttggttg catataaaaa
     5581 gttttatgat ggtaagaaat tgcaatttca catgcttacc attttggctt gttcattcca
     5641 tatttctgct gtaattgtct ttattttgat tccgttttta aaacgcagaa atatatatat
     5701 attaaaaata ctgacaatcg ttggtttagt tttggcaatt tttagtgttt atcctgttga
     5761 ctacttaata cagttactgt cattgctccc tgctggtggt tatatagaaa agataagatg
     5821 gtatagtcag gatgattatg ctggatcagt acttacgttt tcattagtat tcaaagtttt
     5881 tgttgtgctt ttattcgatt acagatttaa atcaataaaa tcacatggtc aaagtcttat
     5941 taatgcgagg gcatatgatt ttatttatac ttcggtttat ttaatgatat tcatggacgt
     6001 ttatcttggt aggtttggta ctattagcac caggcttgac gtatacttta taccatgttt
     6061 tttaatagct cttaatcatt taataaatga acacaaacaa ggtgtgagtc gtttcatatt
     6121 cttcttcgtt gtcatggttt actttactat aaattatctc agtattatga acggatatta
     6181 cttcgagaaa ttttacagtc cttatcaaaa ttatataact gaatttttaa atccgggcag
     6241 ttatagtgat agagggtggg atgttagata ttatttcagc aataaggaat tattgcagtg
     6301 aatcttttga ttaatgctag taatctgtat gttggtggag gagtccaggt agcaatttct
     6361 gtactggaag agttatctga ttcatcattt tcttttattg cggttgtttc accagttgtt
     6421 tattcgcagt taagcgatga tgcagcatca tgttgtatag tgattgaatc ttctccatca
     6481 aaattgttga attttaaagt tagaaggcaa ctcgacgata tagttaaaaa aaatgatatt
     6541 tctgtagttt tcacaatctt cggtcctagc tattggtctc cgaaaaatgt taaacatgct
     6601 attggttttg cgctcccttg gttgatttat gatattgaat atatatttaa aaaattgact
     6661 ttaaaagcta aattgaaatt ttgcatttta aaattattac agccatatta cttcaagaaa
     6721 aatgccgact taatttttac tgaaacagat gatgtcaact tgcgggtaac aaaacttctt
     6781 aactttgaaa aagaacaagt ctacactgtt tcaaacacac ttaatggttt attaaaaaat
     6841 tcaaactgtt atgattacag cattttggat agattaccta cgaaagaacc aaatgatatt
     6901 tggttggtaa ctatttctca taattaccct cataagaact tagaagtcat taaagaatta
     6961 gtaacagtat tgccaccttg ttataagttt attttaacag tttcaagtga ttttttgcag
     7021 ctagtcccaa aagagcatag agagcgagtc atcaccatag gtaatgcaac acttagtcaa
     7081 tgtgctccgt tgtatgaagt ttgtgatgga ttattcatgc caacactttt agaatgcttt
     7141 agtgcttctt acttagaagc aatgtacatg aaaaaaataa tcttcacttc tgatctgcct
     7201 tttgctcaca ctgtttgcaa agatgcagca ttttactttg ctcctcatga tgttgaaaat
     7261 attaggagta cacttgttaa tggctttcaa aacaaagaaa ttcttaacca taaattaaat
     7321 gaaggttcga aaatttatga aagttttcct tctgcgaaag ctcgagcatt gcagtatata
     7381 gatataataa aatccaactt ggtataaatt gtagattttg aggtgttaaa aatgtttaaa
     7441 gataaggttt tacttattac tggcggaaca gggtctttcg gtaacgctgt acttaatcgt
     7501 tttcttgaaa ctgatattaa agaaataagg attttttctc gcgatgaaaa aaaacaagac
     7561 gatatgcgga aaaaatataa taattctaag ttaaaattct acattgggga tgtaagggat
     7621 tattccagca ttctaagcgc cgcacgtggc gtcgacttta tataccatgc tgctgcatta
     7681 aaacaagttc cgtcatgtga atttcatcct ctggaagcag tgaaaactaa cgtattaggt
     7741 acagagaatg tgcttgaagc agcaattgca aaccaggtta aacgtgtcgt ttgcttaagc
     7801 acggataagg cagtatatcc gattaatgcc atgggtattt ccaaagcaat gatggaaaaa
     7861 gtgatggtcg caaaatcacg taatgttaat agcgacaaaa ctgtgatttg cggtactcgc
     7921 tacggcaacg tgatggcttc acgtggttcc gttattccct tattcgttga tctgatcaaa
     7981 gcgggcaaag cgctgacgat cactgaccca aatatgaccc gttttatgat gacgcttgaa
     8041 gatgctgtcg atctggtcct atatgcgttt gaacatggta ataatggcga tatatttgtc
     8101 caaaaagcgc ctgctgcaac gattgaaaca ttagccattg ctctcaaaga acttcttaat
     8161 gttgagcaac atcctgttaa tgttatcgga acgcgccatg gtgagaaact ttatgaagcg
     8221 ctgcttagcc gtgaagagat gattgccgcc attgacatgg gggattacta ccgtgttcca
     8281 ccagacctgc gcgatcttaa ctatggaaaa tacgtagaac aaggtgatag ccgaatctca
     8341 gcagtagagg attataactc tcacaataca cagcgactgg atgttgaagg gatgaaaacg
     8401 cttcttttga aattaccttt tattcgtgca ctgcgtgcgg gtgaacatta cgatctggat
     8461 gcctaatatg aaaatcctga ttactggagc tgatggtttt attggacgta acctgtgctt
     8521 acgccttcag gaagcaggct actgtgacct tgttaagatt gaccgcggtt caagtgcggc
     8581 tgatctggaa actggccttc aagatgctga ttttgtctat catctcgcag gtatcaatcg
     8641 acctaaaaac gttgatgaat ttgccgaggg gaatagcaat ctgactcaac agattgttga
     8701 ttgtctttta gccaagcata aaagcatacc tattatgatc agttcttcca ttcaggctga
     8761 actggttaat gcttatggtc aaagtaaagc tgcagcagaa aaacatattg aacgctatgc
     8821 agctgaaagc ggtgcagctt attttattta tcgttatccg aatgtttttg gtaagtggtg
     8881 taagcctaat tataattcgt tcgtggcgac cttttgccat aatattgcca acaatatcga
     8941 tatcacgatc aatgactcct tcgcgcctgt taatcttgtt tatattgatg atgtctgttc
     9001 tgatgcgata aagctcttgt ctggaaaggt tgaaagcggg tacaaaactg ttaagccagt
     9061 atattcgacg acagtaggtg aggtggcgga attactttat cgcttcaaag aaagccgttc
     9121 cactcttgtc accgaggctg taggaacagg gttcatccgc gcgctgtatt cgacgtggtt
     9181 aagttatctc ccagccgata tgtttgcgta ttcagttccc tcttacggag acgcccgagg
     9241 ggttttttgt gaaatgttaa aaaccccttc agcggggcag ttttcatttt ttacagcgca
     9301 tcccggcatt acacgtgggg gtcattatca tcacaccaaa aatgagaagt tcctggtcat
     9361 tcgcggccag gcatgcttta ggtttgaaca tgtgattacc ggtgagcgat atgagatgaa
     9421 tgtttcctca gatgagttca aaatcgttga gacagtcccc ggctggacac atgatgttac
     9481 aaatattgga gcggatgaat tgatagtcat gctgtgggca aacgaaattt tcagtcgcga
     9541 tgagcctgat actattgcga gacctctgta atgaaaaaac taaaaattat gtctgttgtt
     9601 ggtacgcgtc ccgagattat ccgtctgtca cgcgttctcg ctaagcttga tgaacactgc
     9661 gagcatattc ttgtccatac tggtcaaaac tatgattatg agttaaacga agtattcttt
     9721 aatgaccttg gtgtacgaaa acccgattac tttttaaatg ctgctgggaa aaatgctgca
     9781 gaaaccatcg gtcaggttat catcaaagtt gatgaggtac ttgaatccga aaagcctgaa
     9841 gcaatgttgg tgctgggtga tacaaactcc tgtatttctg caattccagc caaacgccgt
     9901 aaagtgccta tcttccatat ggaagctggc aatcgttgtt tcgatcagcg cgtcccggaa
     9961 gagaccaatc ggcgcattgt agaccatacc gctgacatca atatgaccta cagcgatatt
    10021 gcacgtgaat accttcttgc tgaaggcctc ccagcggacc gaattattaa aaccggtagc
    10081 ccaatgtttg aggtacttac gtattatatg cctcaaatcg ataattcgga tgtactgtcg
    10141 cgtctgaacc tgcgttcagg cgaatttttc gtcgtcagcg cgcatcgtga agagaatgtt
    10201 gattctccca aacagctagt caagcttgcg actattctca atactattgc tgaaaaatat
    10261 gatttgccgg ttattgtatc cactcatccg cggacacgta atcgtattaa cgagcaaggg
    10321 attgaattcc atccaaatat taatctactg aaaccgttag gtttccatga ttacaaccat
    10381 ttgcagaaaa attcacgtgc tgtgctgtct gacagcggta cgataactga agaatcttcc
    10441 attatgaatt tcccggcggt aaatattcgg gaagcacatg agcgtccaga gggctttgaa
    10501 gaagcctctg tcatgatggt gggcctggat tgtgaacgtg ttctacaggc actggatatt
    10561 ctggcaacac agccccgcgg cgaaacccgt cttttacgac aagtaagtga ctacagcatg
    10621 cctaatgtgt cagataaagt cgtcagaatc gttcattctt atactgatta tgtcaagcga
    10681 gtcgtctgga aagaatactg atgaaacttg ctttaatcat agatgattac ctgcccaata
    10741 gtacgcgagt tggtgcaaaa atgtttcatg aattggctca ggaatttatt cgccgtgggc
    10801 atgatgttac ggtaattaca cctgacatct gtcttcagga tgatgtgtcc tttagcacct
    10861 tccagggggt caagacatgg cgtttcaaaa gtgggcctct caaggatgtg agcaaaattc
    10921 aacgagccat caatgaaaca cttctatcct atcgtgcctg gaattccatt aaaagccaga
    10981 taaaaaaaga gacttttgat ggggtagttt attactcacc ctccattttc tgggggcact
    11041 tagtcaagaa aattaaatct cgttgccagt gtccggctta cctgattttg agggatatgt
    11101 tcccgcaatg ggtgatagac gctggcatgt taaaagccgg ttccctcatc gaacgctatt
    11161 tccgtgtttt cgaaagatca tcttatcgcc aggcaaaccg tatcgggctg atgtcagata
    11221 agaatcttga ggtctttcgg gttaacaaca aaggttatcc ttgtgaggtt ttgcgtaact
    11281 gggcttctct cacgccgacg gtaccacccc agggttatat cccattgcgt cagcgtcttg
    11341 gccttgatga taaagttatt ttcttctatg gagggaatat cggtcatgcg caggatatgg
    11401 ggaatctgat gcgtcttgct cgaaaaatgg ctgagcatcc gcaagcccat tttttattta
    11461 tcgggcaggg ggatgaagtt gaattaatca attctttggc tgccgagtgg tcattgccca
    11521 acttcacgta tttggcctct gtcaatcagg atgaatttaa atttatcctc tcggaaatgg
    11581 atataggctt gttttcactt tccgccagac attcttcgca taatttcccg ggtaaattgc
    11641 tagggtatat ggttcagtcc ttacctatac taggcagtgt gaatgctggt aacgatttgc
    11701 ttgatgtcgt caatcaaaat aatgccggat taattcatat caatggtgag gatgacaagc
    11761 ttcatgagtc cgcactgtta atgcttaagg atgttgctgc gcgacgtcaa ttcggcttag
    11821 gcgcgaatgc attattgaga gaacagttct ccgttgagtc tgcggcacag actatagaaa
    11881 tgaggttaga ggcatgtaat gcgactcatt gatactcacc aacttgaagc tttatacgaa
    11941 caagccggaa aatctgcacg cttgcgcgct catctcttat tgcacaattc gcaccgagag
    12001 aaagtgcaac gtctgctcat tgccttggtt cagggcagct atgtcgatcc gcatttccat
    12061 gaacttcctc atcaatggga gatgtttgtc gttatgcagg ggcaggttca agtctgtttg
    12121 tacggcaaag atggcgaaat cattaatcaa tttgtcgctg gagagaatac tgcaataagc
    12181 gtagtcgagt tttctccggg tgatatacac agtgtcgaat gtctctctcc gagagcatta
    12241 atgatggaag tgaaagaagg gccttttgat ccttcctttg ccaaggcgtt catctaacgc
    12301 ccctctgaat cgcatcttcc gctatctact caggctcatc ctgagttaac atctaagcca
    12361 catttcaagc cgcgcacagt cgcggcgacc acacctgaca ggagtatgta atgtccaagc
    12421 aacagatcgg cgtcgtcggt atggcagtg
//
